JP6243446B2 - 共振センサを使用して流体を分析するための方法 - Google Patents
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Description
Claims (25)
- 試料を分析するための方法であって、
複数の共振回路を有する検知領域と複数の調整要素とを有するセンサアセンブリを提供するステップと、
前記センサアセンブリを、試料を含む環境に曝露するステップと、
前記センサアセンブリを用いて生成される1以上の周波数を使用して試料を探査するステップと、
前記複数の共振回路の間での切替えを行うステップと、
前記センサアセンブリの測定されたスペクトル周波数範囲にわたって、センサ応答のインピーダンスを求めるステップと、
前記センサアセンブリのインピーダンスの測定値を、前記試料の少なくとも1つの環境特性に関係付けるステップと
を含む、方法。 - 複数の共振回路と、共振回路を有しない複数の調整要素とを使用してセンサ信号を測定するステップを含む、請求項1記載の方法。
- 回路共振の周波数範囲にわたって前記センサ応答を測定するステップを含む、請求項1記載の方法。
- 前記回路共振の周波数範囲の外部の周波数範囲にわたって前記センサ応答を測定するステップを含む、請求項1記載の方法。
- 前記方法は、前記試料に外部刺激を与えるステップを含み、
前記外部刺激は、電場、磁場、放射、音場、機械場、温度場、電離放射、薬理学的刺激、又はそれらの組合せを含む、請求項1記載の方法。 - 前記センサアセンブリは複数の電極対を備え、
前記方法は、時間的に変化する電磁信号によって前記複数の電極対のうちの少なくとも一方の電極対を電気的に励起するステップを含む、請求項1記載の方法。 - 前記時間的に変化する電磁信号は、電力、周波数、振幅、又はそれらの組合せによって変調される、請求項6記載の方法。
- 前記センサアセンブリは複数の電極対を備え、
前記方法は、電力が変化する電磁信号によって前記複数の電極対のうちの少なくとも一方の電極対を電気的に励起するステップを含む、請求項1記載の方法。 - 前記センサアセンブリは複数の電極対を備え、
前記複数の電極対の少なくとも一つの電極はグローバル電極として機能するように構成されており、前記複数の電極対の他の電極は、局所電極として機能するように構成されており、前記グローバル電極は、局所電極において応答を誘発するように構成されている、請求項1記載の方法。 - 4電極構造をグローバル電極によって駆動するステップと、局所電極によって読取りを駆動するステップとを含む、請求項9記載の方法。
- 前記センサアセンブリは複数の電極対を備え、
前記複数の電極対は、時分割多重化、符号分割多重化、周波数分割多重化、又はそれらの組合せによってアドレスされる、請求項1記載の方法。 - 前記試料は、タンパク質、ウイルス種、細胞、細菌、又はそれらの組合せを含む、請求項1記載の方法。
- 外部刺激の存在下又は不在下の前記試料の材料の周波数依存誘電特性に基づいて、前記1以上の周波数を選択するステップを含む、請求項1記載の方法。
- 外部薬理学的刺激の存在下又は不在下の前記試料の材料の周波数依存誘電特性に基づいて、前記1以上の周波数を選択するステップを含む、請求項1記載の方法。
- 前記センサアセンブリを提供するステップは、
ウェルプレートを提供するステップと、
前記ウェルプレートのウェルごとに単一の検知領域を堆積するステップと
を含む、請求項1記載の方法。 - 前記センサアセンブリを提供するステップは、
ウェルプレートを提供するステップと、
ウェルプレートのウェルごとに少なくとも2つの検知領域を堆積するステップと
を含む、請求項1記載の方法。 - 前記複数の共振回路のうちの1以上の共振回路に対応する共振周波数を調整するために前記調整要素の1以上の電気的パラメータを調整するステップを含む、請求項1記載の方法。
- 主成分分析、部分最小二乗法、等価回路モデル及び/又は非線形重回帰を使用してセンサ応答をモデル化するステップを含む、請求項1記載の方法。
- 等価回路モデルは、遺伝的アルゴリズムを通じて自動的に構成される、請求項1記載の方法。
- 前記等価回路モデル回帰推量係数は、遺伝的アルゴリズム又は複数のパスを通じて自動的に推定される、請求項19記載の方法。
- 試料を分析するための方法であって、
複数の共振回路を有する検知領域と複数の調整要素とを有するセンサアセンブリを提供するステップと、
前記センサアセンブリを、前記試料を含む環境に曝露するステップと、
前記センサアセンブリによって生成される少なくとも2つの共振を用いて前記試料を探査するステップと、
前記複数の共振回路の間での切替えを行うステップと、
前記センサアセンブリの測定されたスペクトル周波数範囲にわたって、センサ応答の共振インピーダンススペクトルを求めるステップと、
多変量応答係数を得るために、センサ応答の前記共振インピーダンススペクトルに多変量統計分析を適用するステップと、
前記多変量応答係数を、前記試料の少なくとも1つの環境特性に関係付けるステップと
を含む、方法。 - 前記センサアセンブリによって生成される前記少なくとも2つの共振は、基本共振である、請求項21記載の方法。
- 異なる電力レベルのセンサ励起を適用することによって、前記センサアセンブリによって生成される少なくとも前記2つの共振を得るステップを含む、請求項22記載の方法。
- 前記センサアセンブリによって生成される前記少なくとも2つの共振は、基本共振と、前記共振回路の少なくとも1つの高調波共振とを含む、請求項22記載の方法。
- 前記センサアセンブリによって生成される前記少なくとも2つの共振は、前記共振回路の高調波共振を含む、請求項22記載の方法。
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PCT/SE2013/051590 WO2014104965A1 (en) | 2012-12-28 | 2013-12-20 | Methods for analysis of fluids using resonant sensors |
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