JP6186806B2 - Acicular body manufacturing method and acicular body - Google Patents

Description

  The present invention relates to a method for manufacturing an acicular body and an acicular body.

  The percutaneous absorption method, which is a method of infiltrating a drug from the skin and administering the drug into the body, is used as a method for easily administering the drug without causing pain to the human body. Some drugs are difficult to administer by transdermal absorption. As a method for efficiently absorbing these drugs into the body, attention has been drawn to a method of perforating the skin using microscopic needle-like bodies and directly administering the drug into the skin. According to this method, it is possible to easily administer a drug subcutaneously without using a special medication device (see Patent Document 1).

  The shape of the fine needle-like body used at this time is required to have a sufficient fineness and tip angle for piercing the skin, and a sufficient length for penetrating the drug solution under the skin, The diameter is about several μm to several hundred μm (specifically, for example, about 1 μm to 300 μm), the length is a length that penetrates the stratum corneum that is the outermost layer of the skin and does not reach the nerve layer, specifically Is preferably about several tens of μm to several hundreds of μm (specifically, for example, about 10 μm to 1000 μm).

  It is known to use various materials as the material for the needle-shaped body described above. For example, a method for producing a needle-shaped body by filling a mold with an aqueous solution of a water-soluble polysaccharide such as glycogen or dextrin and drying the mold. It is disclosed (see Patent Document 2).

  Furthermore, as a method for producing a needle-like body, it has been proposed to prepare an original plate using an etching method, form a mold from the original plate, and perform transfer processing molding using the template (see Patent Document 3). .

  Moreover, as a material used for the mold of the needle-like body, a silicone resin excellent in heat resistance and solvent resistance is known (see Patent Document 4).

JP-A-48-93192 International Publication No. 2006/080508 Pamphlet International Publication No. 2008/004597 Pamphlet JP 2009-254876 A

  When the needle-like body is used by puncturing the skin, the needle-like body directly touches the living body, so it is necessary to ensure hygiene. One way to ensure hygiene is to make it disposable. By making the mold disposable, the risk of contamination due to repeated use of the mold can be eliminated. However, in the mold made of the silicone resin disclosed in Patent Document 4, since the silicone resin is an expensive resin, it is not practical to make it disposable because an increase in the cost of manufacturing the needle-like body is a problem.

  Then, this invention makes it a subject to provide the manufacturing method of the acicular body using the casting_mold | template which is cheap and disposable, and a hygienic and cheap acicular body.

In order to solve the above-described problem, the invention according to claim 1 is a method for manufacturing a needle-like body having a protrusion on at least one surface of a base, and the needle-like body having a protrusion on one surface of the base. A step of preparing an original plate, transferring a projection / recess inversion pattern of the protrusions of the acicular body original plate to prepare a mold, a step of preparing a needle-like body forming liquid containing a needle-like body material, and the needle-like body a step of filling the needles forming liquid containing material into the mold, drying the needles forming solution containing the needle-like material in a state of being filled in the mold, solidifying the needle-shaped material And a step of peeling the solidified needle-shaped material from the mold to form a needle-shaped body, and the mold contains an ethylene / vinyl acetate copolymer resin, and the needle-shaped reversal pattern of the needle-shaped body It was set as the manufacturing method of the acicular body characterized by providing.

  According to a second aspect of the present invention, there is provided the needle-shaped body manufacturing method according to the first aspect, wherein the needle-shaped body material includes a biocompatible material.

  According to a third aspect of the present invention, at least one selected from the group consisting of chitin, chitosan, chitosan derivatives, collagen, gelatin, carboxymethylcellulose, hydroxypropylcellulose, hydroxypropylcellulose derivatives The needle-shaped body manufacturing method according to claim 1, wherein the needle-shaped body manufacturing method is provided.

  In the method for producing a needle-like body of the present invention, a mold containing an ethylene / vinyl acetate copolymer resin is used as a mold material. Since ethylene / vinyl acetate copolymer is about one-tenth the price of silicone resin, it can be an inexpensive method for producing needle-like bodies. Furthermore, since it is inexpensive, it can be used as a disposable mold and can be excellent in hygiene. The needle-like body obtained from the manufacturing method using the disposable mold can be a hygienic and inexpensive needle-like body. In addition, since the ethylene / vinyl acetate copolymer has a low glass transition point and high flexibility even at low temperatures, the needle-like body can be produced even at low temperatures by using the mold of the present invention.

FIG. 1 is a perspective view of the needle-shaped body of the present invention. FIG. 2 is a schematic view of the needle-shaped body of the present invention. FIG. 3 is an explanatory view of the method for producing a needle-shaped body of the present invention. FIG. 4 is a schematic view of an embodiment of a mold used in the method for producing a needle-like body of the present invention. FIG. 5 is an explanatory diagram of the needle-shaped body manufacturing apparatus of the present invention. FIG. 6 is an explanatory diagram of the needle-shaped body manufacturing apparatus of the present invention. FIG. 7 is an explanatory view of the needle-shaped body manufacturing apparatus of the present invention. FIG. 8 is an explanatory diagram of the needle-shaped body manufacturing apparatus of the present invention.

<Needle body>
In FIG. 1, the perspective view of the needlelike object of the present invention was shown. The needle-like body 101 of the present invention includes a base 103 and a protrusion 105 protruding from the first surface of the base 103. The needle-like body of the present invention includes a biocompatible material.

  The biocompatible material in the present invention means a material having a property capable of performing a target function without causing harm to the living body. Water-soluble polymers, water-insoluble polymers, biopolymers, and the like that do not harm the living body are equivalent.

  A known biocompatible material can be used as the biocompatible material contained in the needle-shaped body material of the present invention. Specific examples include alginate, curdlan, chitin, chitosan, glucomannan, polymalic acid, collagen, collagen peptide, hydroxypropylcellulose, gelatin and the like. Among them, the biocompatible material used in the needle-shaped body of the present invention is selected from the group consisting of chitin, chitosan, chitosan derivatives, collagen, gelatin, carboxymethylcellulose, hydroxypropylcellulose, hydroxypropylcellulose derivatives. preferable.

  The chitin, chitosan and chitosan derivatives used as the needle-shaped body material of the present invention have biocompatibility. Although there is no clear boundary line between chitin and chitosan, generally, chitin having a deacetylation of 70% or more is called chitosan. Deacetylation can be appropriately performed by a known method. As chitin, chitosan and chitosan derivatives having biocompatibility, those derived from crustaceans such as salmon and shrimp, those derived from plants produced by mycelia and microorganisms, and those starting from those can be used. Chitosan, chitin / chitosan and chitin / chitosan derivatives have a cosmetic effect on the skin and have a bactericidal and antibacterial effect, and therefore can be preferably used as a needle-shaped material used in the needle-shaped body of the present invention.

  Moreover, the acicular body of this invention may contain the delivery thing inside. Examples of deliverables include physiologically active substances and cosmetic compositions. In addition, when a material having a fragrance is used as a delivery product, an odor can be imparted during use, which is preferable for use as a cosmetic product. In addition, a delivery thing shall also contain a biologic. Here, the biologic refers to the classification of drugs using raw materials or materials derived from human or animal cells or cell tissues. Further, in the needle-shaped body of the present invention, the delivery product may be applied and held on the surface of the needle-shaped body.

  In addition, when the delivery product is administered with a needle-like body, the delivery product may be applied onto the target skin before or after the needle-like body is punctured into the skin. At this time, the delivery material may be disposed on or inside the needle-like body, and the delivery material may be applied to the target skin surface.

  Further, the needle-shaped body of the present invention may be used in a technique of being used by being buried in the body.

  The shape and arrangement of the needles according to the present invention will be described with reference to FIG.

  2A and 2B are schematic views of the needle-shaped body of the present invention, where FIG. 2A is a top view of the needle-shaped body, and FIG. 2B is a cross-sectional view of the needle-shaped body. FIG. 2B is a cross-sectional view of FIG. 2A taken along line II ′. As shown in FIGS. 2A and 2B, the needle-like body 101 includes a base 103 and a protrusion 105 protruding from the first surface of the base 103.

  The needle-shaped body according to the present invention may include one protrusion on the base, or may include a plurality of protrusions on the base. When a plurality of protrusions are provided, the protrusions may be provided in an array.

  In particular, it is preferable that a plurality of protrusions are arranged on the base and arranged in an array. Here, the “array shape” indicates a state in which the protrusions are arranged, and includes, for example, a pattern such as a lattice arrangement, a close-packed arrangement, a concentric arrangement, and a random arrangement.

  The shape of the protrusion of the present invention is not limited to the form shown in FIGS. 1, 2 (a) and 2 (b), and the shape and dimensions may be freely designed depending on the application. For example, it is selected depending on the case where the molded needle-shaped body is used for the purpose of promoting percutaneous absorption of a delivery product or for the purpose of percutaneously extracting a substance in a living body to the outside of the living body. Moreover, it selects from a viewpoint of skin puncture performance. Specifically, a cone shape such as a cone or a pyramid, a columnar shape such as a cylinder or a prism, or a pencil shape combining the cone shape and the columnar shape (the body portion is a columnar shape and the tip portion is a cone shape), etc. The shape can be selected. Note that a constriction or a step may be formed on the side wall of the protrusion.

  In particular, when the length of the perforations by the protrusions is set to “depth that penetrates the stratum corneum and does not reach the nerve layer”, the delivery product or the cosmetic composition can be delivered to a deeper position than the stratum corneum. Since the perforations formed in the stratum corneum are closed over time, the delivered product and the cosmetic composition delivered under the stratum corneum are retained in the living body in a state of being barriered to the stratum corneum from the outside. For this reason, the delivery product and the cosmetic composition can reduce exfoliation due to the metabolism of the stratum corneum and washing such as skin care, and can maintain the makeup state for a long time.

  In particular, when the depth of perforation by the protrusions is kept “in the stratum corneum”, the delivery product and the cosmetic composition can stay in the stratum corneum. Since the stratum corneum is constantly generated newly by metabolism, the deliverables and cosmetic compositions in the stratum corneum are discharged out of the body with time. For this reason, for example, the makeup state and the applied state can be easily released by washing the skin or peeling the skin.

In particular, when the depth of perforation by the protrusion is kept at “the length that penetrates the stratum corneum and does not reach the nerve layer”, the length H of the protrusion is specifically 200 μm or more and 700 μm or less, more preferably. Is in the range of 200 μm to 300 μm, more preferably 200 μm to 300 μm.

  In particular, when the perforations are held in “in the stratum corneum”, the length H of the protrusion is specifically 30 μm or more and 300 μm or less, more preferably 30 μm or more and 250 μm or less, and further preferably 30 μm or more and 40 μm or less. It is preferable to be within the following range.

  Further, when the protrusions according to the present invention are arranged in an array on the substrate, the lengths of the protrusions may or may not be the same. When the lengths of the protrusions are different, for example, (1) the protrusions only on the array-shaped outer periphery can be suitably brought into contact with the curved surface, and (2) the protrusions on the array-shaped outer periphery are short. By doing, there are advantages such as the mechanical strength of the needle-like body at the outer peripheral portion that is easily damaged can be reinforced.

  The width D of the protrusion in the needle-like body of the present invention is preferably in the range of 1 μm to 300 μm. The width D of the protrusion is preferably determined in consideration of the depth of the puncture hole formed when the needle-like body is punctured within the above range.

  The width D of the protrusion is the maximum length of the protrusions in contact with the base when the protrusion is projected in parallel with the base surface. For example, when the projection is conical, the diameter of the circle on the surface in contact with the projection and the base is the width D. When the protrusion is a regular quadrangular pyramid, the square diagonal line of the surface in contact with the protrusion and the base is the width D. When the protrusion is a cylinder, the diameter of the circle on the surface in contact with the protrusion and the base is the width D. When the protruding portion is a regular quadrangular prism, the square diagonal line of the surface in contact with the protruding portion and the base is the width D.

  In the needle-like body according to the embodiment in which the protrusion has a tip angle like a cone shape, the tip angle θ of the protrusion is 5 ° or more and 30 ° when the protrusion of the needle-like body penetrates the stratum corneum during perforation. In the following, it is more preferable that the angle be in the range of 10 ° to 20 °. In addition, when the protrusion of the needle-like body is held in the stratum corneum at the time of drilling, the tip angle is preferably 30 ° or more, more preferably 45 ° or more. The tip angle θ indicates the largest angle (vertical angle) when the protrusion is projected in parallel with the base surface.

  Further, the base body and the protrusions constituting the needle-like body may be formed of the same composition material or different composition materials. By forming the base body and the projecting portion with the composition having the same composition, the base body and the projecting portion can be suitably formed by integral molding. In addition, when producing the acicular body of this invention with a different material, it is necessary for the protrusion part of an acicular body to contain biocompatible material and glycerin at least.

  Next, the manufacturing method of the acicular body of this invention is demonstrated. FIG. 3 shows an explanatory view of the method for producing the needle-shaped body of the present invention.

  The method for producing a needle-like body of the present invention is a method for producing a needle-like body having a protrusion on at least one surface of a substrate, and a step of preparing a needle-like body forming liquid containing a needle-like body material; Filling the mold with a needle-shaped body forming liquid containing the needle-shaped body material; and drying the needle-shaped body material with the needle-shaped body forming liquid containing the needle-shaped body material filled in the mold. A step of solidifying, and a step of peeling the solidified needle-shaped material from the mold to form a needle-shaped body, and the mold includes an ethylene / vinyl acetate copolymer resin, and the needle-shaped irregularities A reverse pattern is provided.

<Mold manufacturing process>
FIG. 3A and FIG. 4 show examples of a mold used for manufacturing the needle-like body in the needle-like body manufacturing method of the present invention. The mold 201 includes a concave portion 203 having a concave / convex pattern of protrusions and a concave region 205 formed of a region having the concave portion 203 formed on the first surface. Further, as shown in FIG. 4, a mold substrate 209 may be provided.

  The shape of the mold is not particularly limited as long as it has a shape capable of holding the needle-shaped body material. Specifically, for example, it may be a polygon such as a quadrangle, a triangle, a circle, an ellipse, or the like, and may be any assumed shape.

  The first surface of the mold is not particularly limited as long as it has a recess. Specifically, for example, a flat surface, a curved surface, a step structure, or the like may be used. Further, the first surface of the mold may be subjected to surface processing suitable for the needle-shaped body material to be used and the manufacturing process state.

  As the material of the mold, an ethylene / vinyl acetate copolymer is used for the recessed region 205 in contact with the acicular material. The ethylene / vinyl acetate copolymer is a general-purpose resin having biocompatibility, and is a material excellent in transferability of a fine structure. The price is as low as about 1/10 compared with a silicone resin excellent in transferability. Therefore, it can be set as a disposable and inexpensive mold, and a mold excellent in hygiene can be obtained. Furthermore, since it has flexibility even at a low temperature as compared with a general-purpose thermoplastic resin such as polypropylene and polystyrene, a needle-like body can be suitably produced even at about room temperature.

  As another embodiment of the mold, a mold substrate 209 shown in FIG. 4 may be provided. The material of the mold substrate is not particularly limited as long as it has heat resistance, heat conduction, mechanical strength and the like as the substrate. For example, metals and resins, for example, (1) silicon, (2) metal materials such as nickel, aluminum, stainless steel and titanium, (3) ceramics such as alumina, aluminum nitride, machinable ceramics and glass, (4) Synthetic resins such as polyatar, polycarbonate, polystyrene, acrylic resin, and fluororesin may be used. By using a mold substrate as shown in FIG. 4, the deformation of the mold can be suppressed, and further, the operability of the mold can be improved, so that the stability of the shape of the needle-like portion of the obtained needle-like body is improved. Can be improved.

  In the present invention, by using an ethylene / vinyl acetate copolymer as a material of a mold, it was possible to obtain a needle-like production method excellent in hygiene and inexpensive. Furthermore, the needle-like body obtained from the manufacturing method using a disposable mold can be a hygienic and inexpensive needle-like body. In addition, since the ethylene / vinyl acetate copolymer has a low glass transition point and high flexibility even at low temperatures, the needle-like body can be produced even at low temperatures by using the mold of the present invention. In order to remove the solvent, the method for producing a needle-shaped body according to the present invention produces a needle-shaped body using a liquid needle-shaped body forming liquid in which a needle-shaped body forming material is dissolved and / or dispersed in a solvent. A drying process is provided. When the mold-like acicular body forming liquid is dried by the drying process, the mold may be thermally deformed. From this point, the mold is preferably disposable.

  As a means for forming the recess 203 in the mold, a known microfabrication technique may be appropriately selected and used. Examples of such a microfabrication technique include a mold taking method, a lithography method, a wet etching method, and a dry process. Includes etching, sandblasting, laser processing and precision machining. For example, a silicon needle-like body made of silicon using a lithography method, a wet etching method, a dry etching method, a cutting method or the like from a silicon substrate and having a protrusion on one surface of the base is manufactured. It is also possible to produce a mold by a mold making method using as a master.

<Needle formation liquid preparation process>
Next, as shown in FIG. 3A, the acicular body material liquid 207 is prepared. In the needle-shaped body manufacturing method of the present invention, the order of the “mold manufacturing process” and the “needle body forming liquid preparation process” may be performed in either order.

  A biocompatible material can be used for the needle-shaped body material of the present invention. More preferably, the biocompatible material contains at least one selected from the group consisting of chitin, chitosan, chitosan derivatives, collagen, gelatin, carboxymethylcellulose, hydroxypropylcellulose, and hydroxypropylcellulose derivatives.

  The needle-shaped body material made of a biocompatible material is dissolved and / or dispersed in a solvent to form a moldable liquid needle-shaped body forming liquid. The solvent is selected depending on the chemical properties of the biocompatible material. Specifically, water, polyhydric alcohols such as ethylene glycol and glycerin, methyl alcohol, ethyl alcohol, propyl alcohol, and the like can be used. A mixture of these solvents can also be used. In addition to the biocompatible material and the solvent, the needle-like body forming liquid may contain other materials in order to make the needle-like body material into a liquid that can be filled in the mold. For example, when chitosan is used as the biocompatible material, it is necessary to contain an organic acid such as formic acid or acetic acid in order to dissolve chitosan in water. In addition, in the needle-like body forming liquid adjusting step, after each material is mixed, deaeration or the like may be performed.

  In addition, there is no restriction | limiting in the density | concentration of the biocompatible material in an acicular body formation liquid, You may select arbitrarily in consideration of the characteristic of the biocompatible material to be used. When the concentration is in the range of 1% by weight or more and 20% by weight with respect to the needle-like body forming liquid, the needle-like body can be more suitably manufactured.

<Filling process>
Next, as shown in FIG. 3B, the mold 201 is filled with the needle-like body forming liquid 207.

  There is no restriction | limiting in particular about the filling method of the said acicular body formation liquid. For example, as a filling method, an injection molding method, an extrusion molding method, an imprinting method, a casting method, an ink jet method, a dispenser method, a spin coating method, a filling method using centrifugal force, or the like can be used. Moreover, when supplying the needle-shaped body forming liquid to the intaglio, the environment around the intaglio may be reduced or reduced in vacuum.

<Drying process>
Next, as shown in FIG. 3C, the needle-like body forming liquid 207 filled in the mold 201 is dried. There is no restriction | limiting in particular about the drying method of the acicular body formation liquid. For example, heat drying, ventilation drying, vacuum drying, etc. can be used. By drying, the needle-shaped body material is solidified, and the needle-shaped body 101 is obtained.

<Peeling process>
Next, as shown in FIG. 3 (d), the mold 201 is peeled from the needle-like body 101. There is no restriction | limiting in particular about the peeling method of the said acicular body, A well-known physical means can be used.

  As described above, the needle-like body 101 including the base body 103 and the protrusion 105 shown in FIG.

  According to the manufacturing method described above, a hygienic and inexpensive needle-like body can be obtained.

  Specifically, a manufacturing process of a mold having a concave portion formed of a concave-convex inversion pattern of the shape of the convex portion of the needle-like body using an ethylene / vinyl acetate copolymer, and a needle-like body forming liquid containing a biocompatible material Preparation process for preparing needle-shaped body forming liquid, filling process for filling the mold with the needle-shaped body forming liquid, drying process for drying the needle-shaped body material forming liquid filled in the mold, and peeling the needle-shaped body material from the mold What is necessary is just to provide the peeling process of obtaining a needle-like body.

  As mentioned above, the manufacturing method of the acicular body of this invention can be implemented.

  The method for producing a needle-like body of the present invention has an effect that the mold contains an ethylene / vinyl acetate copolymer resin and the needle-like body can be produced at low cost. The needle-shaped body manufacturing method of the present invention may be manufactured by a roll-to-roll method. By producing a needle-like body by a roll-to-roll method, the needle-like body can be further manufactured at low cost. FIG. 5 to FIG. 8 show explanatory views of the needle-shaped body manufacturing apparatus of the present invention by the roll-to-roll method.

  In the acicular body manufacturing apparatus of the present invention shown in FIG. 5, the mold 301 is in the form of a film, and the mold is conveyed from the unwinding roll 302 to the winding roll 303. The film-shaped mold passes through the needle-like body forming liquid supply mechanism 305, the drying mechanism 306, and the peeling mechanism 307 when being conveyed to the take-up roll 303. The acicular body forming liquid supply mechanism 305 intermittently applies the acicular body forming liquid to the mold 301. The drying mechanism 306 dries and solidifies the needle-like body forming liquid applied to the film-like mold. The peeling mechanism 307 peels the dried and solidified needle-like body from the film-like mold. Thus, a needle-like body is manufactured.

  In the acicular body manufacturing apparatus of the present invention shown in FIG. 6, the film-shaped mold 301 is conveyed from the unwinding roll 302 to the winding roll 303. The film-shaped mold passes through the needle-like body forming liquid supply mechanism 305 and the drying mechanism 306 when being conveyed to the take-up roll 303. The acicular body forming liquid supply mechanism 305 intermittently applies the acicular body forming liquid to the mold 301. The drying mechanism 306 dries and solidifies the needle-like body forming liquid applied to the film-like mold. Thereafter, the dried and solidified needle-like body is wound around the film-shaped mold by a winding roll while being fixed to the film-shaped mold. Then, it peels from a film-form casting_mold | template and a needle-shaped object is manufactured.

  In the acicular body manufacturing apparatus of the present invention shown in FIG. 7, the film-shaped mold 301 is conveyed from the unwinding roll 302 to the winding roll 303. The film-shaped mold passes through the needle-like body forming liquid supply mechanism 305, the drying mechanism 306, and the peeling mechanism 307 when being conveyed to the take-up roll 303. The acicular body forming liquid supply mechanism 305 continuously applies the acicular body forming liquid to the mold 301. The drying mechanism 306 dries and solidifies the needle-like body forming liquid applied to the film-like mold. The peeling mechanism 307 peels the film-like needle-like body from the film-like mold. Thereafter, the multifaceted film-like acicular body is cut.

  In the acicular body manufacturing apparatus of the present invention shown in FIG. 8, the film-shaped mold 301 is conveyed from the unwinding roll 302 to the winding roll 303. The film-shaped mold passes through the needle-like body forming liquid supply mechanism 305 and the drying mechanism 306 when being conveyed to the take-up roll 303. The acicular body forming liquid supply mechanism 305 continuously applies the acicular body forming liquid to the mold 301. The drying mechanism 306 dries and solidifies the needle-like body forming liquid applied to the film-like mold. Thereafter, the dried and solidified needle-like body is wound around the film-shaped mold by a winding roll while being fixed to the film-shaped mold. Thereafter, the film-like needle-like body that has been multifaceted is peeled off from the film-like mold and cut.

  In the needle-like body manufacturing apparatus of the present invention according to the roll-to-roll method described in FIGS. 5 to 8, a mold forming mechanism may be provided before the needle-like body forming liquid supply mechanism 305. From the wound ethylene-vinyl acetate copolymer film, a mold having a needle-like shape inversion pattern is formed by a mold forming mechanism, and the needle-shaped body forming liquid supply mechanism 305 forms a needle-like shape on the formed mold. A body forming liquid can be supplied. In the needle-like body manufacturing apparatus of the present invention according to the roll-to-roll method shown in FIGS. 5 to 8, the film-like mold can be used repeatedly and can be disposable. From the viewpoint of shape stability of the surface or needle, the mold is preferably disposable.

  The needle-shaped body and the method for manufacturing the needle-shaped body of the present invention are not limited to the above-described embodiment, and the needle-shaped body obtained by manufacturing from a manufacturing method including other steps, modifications, and changes that can be inferred by those skilled in the art. The body also falls within the scope of the present invention.

<Example 1>
Hereinafter, an example of the manufacturing method of the needlelike object of the present invention is explained in an example.

1. Production of mold First, using precision machining, a silicon substrate was formed with 100 square pyramids (height: 120 μm, bottom: 38 μm × 38 μm) arranged in a grid of 10 columns and 10 rows at 1 mm intervals. Formed. The 100 protrusions were arranged in a square region having a side of about 9 mm.

  Next, the projections and depressions of the protrusions were transferred to the needle-like body formed of the silicon substrate with an ethylene / vinyl acetate copolymer resin to prepare a mold made of the ethylene / vinyl acetate copolymer resin.

2. Preparation of needle-shaped body forming liquid Next, the needle-shaped body forming liquid was adjusted.
Specifically, after dissolving chitosan and acetic acid in water, deaeration was performed under vacuum to prepare a needle-like body forming liquid. At this time, 5% by weight of chitosan and 2.5% by weight of acetic acid were prepared with respect to the needle-shaped body material.

3. Needle-shaped transfer molding (filling process, drying process, peeling process)
Next, an aqueous chitosan solution was supplied onto the mold by an ink jet method to fill the recesses. This was left to stand in a normal temperature and humidity environment and dried, and then peeled off from the mold to obtain a needle-like body. When the obtained needle-like body was observed with a stereomicroscope, regular tetragonal pyramidal protrusions were confirmed, and it was confirmed that a mold made of an ethylene / vinyl acetate copolymer resin was excellent in transferability.

<Example 2>
1. Production of mold First, using precision machining, a silicon substrate was formed with 100 square pyramids (height: 120 μm, bottom: 38 μm × 38 μm) arranged in a grid of 10 columns and 10 rows at 1 mm intervals. Formed. The 100 protrusions were arranged in a square region having a side of about 9 mm.

  Next, the projections and depressions of the protrusions were transferred to the needle-like body formed of the silicon substrate with an ethylene / vinyl acetate copolymer resin to prepare a mold made of the ethylene / vinyl acetate copolymer resin.

2. Preparation of needle-shaped body forming liquid Next, the needle-shaped body forming liquid was adjusted.
Specifically, after dissolving hydroxypropyl cellulose in water, deaeration was performed under vacuum to prepare an acicular body forming liquid. At this time, it prepared as 5 weight% of hydroxypropyl cellulose with respect to the acicular material.

3. Needle-shaped transfer molding (filling process, drying process, peeling process)
Next, an aqueous hydroxypropylcellulose solution was supplied onto the mold by an ink jet method to fill the recesses. This was left to stand on a hot plate at 40 ° C. for several hours and dried by heating, and then peeled off from the mold to obtain a needle-like body. When the obtained needle-like body was observed with a stereomicroscope, regular tetragonal pyramidal protrusions were confirmed, and it was confirmed that a mold made of an ethylene / vinyl acetate copolymer resin was excellent in transferability.

  From the above, it was possible to produce a needle-like body made of the sanitary and inexpensive biocompatible material of the present invention.

The acicular body of the present invention can be used in various fields that require fine acicular bodies. For example, application can be expected as a needle-like body used for MEMS devices, optical members, sample jigs, drug discovery, cosmetics, medical uses, cosmetic uses, and the like.

101 ... acicular body 103 ... base 105 ... projection 201 ... mold 203 ... recess 205 ... recess area 207 ... acicular body forming liquid 209 ... mold substrate 301 ... Mold 302 ... Unwinding roll 303 ... Winding roll 305 ... Needle-like body forming liquid supply mechanism 306 ... Drying mechanism 307 ... Peeling mechanism

Claims (3)

A method for producing a needle-like body having a protrusion on at least one surface of a substrate,
Producing a needle-shaped body original plate provided with a protrusion on one surface of the substrate, transferring the unevenness inversion pattern of the protrusion of the needle-shaped body original plate, and preparing a mold;
Preparing a needle-like body forming liquid containing a needle-like body material;
A step of filling the needles forming solution containing the acicular material into the mold,
Drying the needle-like body forming liquid containing the needle-like body material in a state filled in the mold, and solidifying the needle-like body material;
Peeling the solidified needle-like material from the mold into a needle-like body, and
The mold includes an ethylene / vinyl acetate copolymer resin, and includes a needle-like uneven inversion pattern.   The method for producing a needle-shaped body according to claim 1, wherein the needle-shaped body material includes a biocompatible material.   The needle-shaped body material contains at least one selected from the group consisting of chitin, chitosan, chitosan derivatives, collagen, gelatin, carboxymethylcellulose, hydroxypropylcellulose, and hydroxypropylcellulose derivatives. Item 3. A method for producing an acicular body according to Item 2.

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