JP6150108B2 - ヒト多能性幹細胞から肝前駆細胞への分化誘導方法 - Google Patents

ヒト多能性幹細胞から肝前駆細胞への分化誘導方法 Download PDF

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JP6150108B2
JP6150108B2 JP2013058148A JP2013058148A JP6150108B2 JP 6150108 B2 JP6150108 B2 JP 6150108B2 JP 2013058148 A JP2013058148 A JP 2013058148A JP 2013058148 A JP2013058148 A JP 2013058148A JP 6150108 B2 JP6150108 B2 JP 6150108B2
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cells
human
pluripotent stem
differentiation
hepatic progenitor
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JP2013226127A (ja
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稔 富澤
稔 富澤
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Chiba University NUC
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • A—HUMAN NECESSITIES
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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    • C12N2510/00—Genetically modified cells

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  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2013058148A 2012-03-30 2013-03-21 ヒト多能性幹細胞から肝前駆細胞への分化誘導方法 Expired - Fee Related JP6150108B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2013058148A JP6150108B2 (ja) 2012-03-30 2013-03-21 ヒト多能性幹細胞から肝前駆細胞への分化誘導方法
US13/852,627 US20130260458A1 (en) 2012-03-30 2013-03-28 Method of inducing differentiation of human pluripotent stem cell into hepatic progenitor cell

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2012080708 2012-03-30
JP2012080708 2012-03-30
JP2013058148A JP6150108B2 (ja) 2012-03-30 2013-03-21 ヒト多能性幹細胞から肝前駆細胞への分化誘導方法

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JP2013226127A JP2013226127A (ja) 2013-11-07
JP2013226127A5 JP2013226127A5 (https=) 2016-05-12
JP6150108B2 true JP6150108B2 (ja) 2017-06-21

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104830906B (zh) * 2014-02-12 2018-09-04 北京维通达生物技术有限公司 一种重编程获得功能性人肝脏实质细胞的方法
US10006005B2 (en) * 2014-06-27 2018-06-26 National University Corporation Chiba University Culture medium and method for inducing differentiation of pluripotent stem cells to hepatoblasts
CN105779624A (zh) * 2016-04-22 2016-07-20 同济大学苏州研究院 一种用于检测人源性chop基因表达水平的引物组合及其应用技术领域

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US20130260458A1 (en) 2013-10-03

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