JP6135141B2 - UV sterilization method - Google Patents
UV sterilization method Download PDFInfo
- Publication number
- JP6135141B2 JP6135141B2 JP2013007561A JP2013007561A JP6135141B2 JP 6135141 B2 JP6135141 B2 JP 6135141B2 JP 2013007561 A JP2013007561 A JP 2013007561A JP 2013007561 A JP2013007561 A JP 2013007561A JP 6135141 B2 JP6135141 B2 JP 6135141B2
- Authority
- JP
- Japan
- Prior art keywords
- ultraviolet
- sterilization
- intensity
- seconds
- sterilization method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Description
この発明は、紫外線ランプにより室内面に紫外線を照射して殺菌を行うための紫外線殺菌方法に関するものである。 The present invention relates to an ultraviolet sterilization method for sterilizing indoor surfaces by irradiating ultraviolet rays with an ultraviolet lamp.
従来、紫外線による殺菌は、薬品による殺菌の場合とは異なり、薬品が残らないため、食品や医薬品の製造工場における室内空気の殺菌や、各種容器の表面殺菌等に広く使用されている。特に、製造工場のクリーンルーム等のクリーンエリアでは、天井部分に紫外線ランプを設置して夜間に紫外線を照射することにより、空気の自然対流を利用した空気殺菌が行われている。この場合、紫外線照射装置の設計は、必要と思われる紫外線強度と殺菌に必要な照射時間を求め、その値に基づいて紫外線ランプの設置場所や本数を決定していた。 Conventionally, sterilization by ultraviolet rays is widely used for sterilization of indoor air in food and pharmaceutical manufacturing factories, surface sterilization of various containers, and the like, unlike the case of sterilization by chemicals. In particular, in a clean area such as a clean room in a manufacturing factory, air sterilization using natural convection of air is performed by installing an ultraviolet lamp on the ceiling and irradiating ultraviolet rays at night. In this case, the design of the ultraviolet irradiation apparatus obtains the ultraviolet intensity and the irradiation time necessary for sterilization, which are considered necessary, and determines the location and number of the ultraviolet lamps based on the values.
一方、紫外線強度は、紫外線ランプからの照射距離が離れるほど小さくなる。しかしながら、紫外線ランプから大きく離れた地点での殺菌効果、すなわち、ごく低い紫外線強度における殺菌効果については、従来から評価データが存在しなかった。よって、前記紫外線照射装置の設計に際しては、紫外線強度が低いほど、殺菌に要する照射時間が長くなることから、照射時間を短くするために、紫外線ランプを殺菌したい箇所にできる限り近付けるとともに、紫外線ランプの設置本数を多くして、紫外線強度を高く設定する傾向にあった。 On the other hand, the ultraviolet intensity decreases as the irradiation distance from the ultraviolet lamp increases. However, there has been no evaluation data for the sterilization effect at a point far away from the ultraviolet lamp, that is, the sterilization effect at a very low ultraviolet intensity. Therefore, in designing of the ultraviolet irradiation apparatus, both as UV intensity is low, because the irradiation time is longer required for sterilization, in order to shorten the irradiation time, the close as possible to where you want to sterilize the ultraviolet lamp, ultraviolet There was a tendency to increase the UV intensity by increasing the number of lamps installed.
また、この種の紫外線による殺菌方法としては、例えば特許文献１及び特許文献２に開示されるような方法が従来から提案されている。特許文献１に記載の従来方法では、光源から１ｍ以上離れた被照射対象に４００〜４１０ｎｍに光強度の極大を有する近紫外光を、被照射対象の表面における近紫外光の強度を０．８２ｍＷ／ｃｍ２ 以上に保って、少なくとも２時間照射するようにしている。 As this type of sterilization method using ultraviolet rays, for example, methods disclosed in Patent Document 1 and Patent Document 2 have been proposed. In the conventional method described in Patent Document 1, near-ultraviolet light having a maximum light intensity at 400 to 410 nm is applied to an irradiation target 1 m or more away from the light source, and the intensity of the near-ultraviolet light on the surface of the irradiation target is 0.82 mW. / Cm 2 or more, and irradiation is performed for at least 2 hours.
さらに、特許文献２に記載の従来方法では、建物の壁面及び天井面における微生物の存在、もしくは微生物の発生雰囲気をセンサにより検出し、その検出信号に基づいて微生物の種類に応じた最小の殺菌照射時間をマイクロプロセッシングユニット（ＭＰＵ）で算定し、その算定された時間だけ紫外線照射装置を動作させて、紫外線を微生物の存在部位に照射して殺菌するようにしている。この場合、紫外線の照射条件は、ランプ出力１５Ｗ、照射距離１ｍ、紫外線強度０．０４ｍＷ／ｃｍ２として、例えばコウジカビでは照射時間が４０分、アオカビでは照射時間が１５分となるように設定している。ただし、この特許文献２においては、前記紫外線強度の測定位置は記載されていない。 Furthermore, in the conventional method described in Patent Document 2, the presence of microorganisms on the wall surface and ceiling surface of a building or the generation atmosphere of microorganisms is detected by a sensor, and the minimum sterilization irradiation corresponding to the type of microorganisms is performed based on the detection signal. The time is calculated by a microprocessing unit (MPU), and the ultraviolet irradiation device is operated only for the calculated time, and ultraviolet rays are irradiated to the site where the microorganism is present to sterilize. In this case, the ultraviolet irradiation conditions are set such that the lamp output is 15 W, the irradiation distance is 1 m, and the ultraviolet intensity is 0.04 mW / cm 2. For example, the irradiation time is 40 minutes for Aspergillus and 15 minutes for Blue Mold. Yes. However, in this patent document 2, the measurement position of the said ultraviolet intensity is not described.
ところが、これらの従来方法においては、殺菌に要する紫外線強度が高く設定されるとともに、紫外線の照射時間が長く設定されている。このため、紫外線ランプの設置本数が多くなって殺菌設備の導入時のコストが高くなるとともに、消費電力のアップやランプ寿命の低下を招いて、運用コストも高くなるという問題があった。 However, in these conventional methods, the ultraviolet intensity required for sterilization is set high, and the ultraviolet irradiation time is set long. For this reason, there are problems that the number of installed ultraviolet lamps increases and the cost at the time of introduction of the sterilization equipment increases, and the operation cost also increases due to an increase in power consumption and a decrease in lamp life.
この発明は、このような従来の技術に存在する問題点に着目してなされたものであって、ごく低い紫外線強度について殺菌効果を評価することにより判明したものである。その目的は、紫外線ランプの設置本数を減らし、ごく低い紫外線強度で細菌やカビの殺菌を行うことができて、殺菌設備の導入時における設備コスト低減を図ることができるとともに、ごく低い紫外線強度を用いた場合でも照射時間を短縮することができ、消費電力の削減及びランプ寿命の延長を図ることができ、運用コストも低減することができる紫外線殺菌方法を提供することにある。 The present invention has been made by paying attention to such problems existing in the prior art, and has been found by evaluating the bactericidal effect for very low ultraviolet intensity. The purpose is to reduce the number of UV lamps installed and sterilize bacteria and mold with extremely low UV intensity, and to reduce equipment costs when introducing sterilization equipment. It is an object of the present invention to provide an ultraviolet sterilization method capable of shortening the irradiation time even when used, reducing power consumption, extending the lamp life, and reducing the operation cost.
上記の目的を達成するために、この紫外線殺菌方法では、紫外線ランプによって室内面に紫外線を照射する紫外線殺菌方法において、殺菌対象が大腸菌であって、紫外線強度を０．００８〜０．１７０ｍＷ／ｃｍ２ で、照射時間を３分１３秒〜３７秒とすることを特徴としている。
更に、紫外線ランプによって室内面に紫外線を照射する紫外線殺菌方法において、殺菌対象がサルモネラ菌であって、紫外線強度を０．００８〜０．１７０ｍＷ／ｃｍ ２ で、照射時間を３分１２秒〜２１秒とすることを特徴としている。
In order to achieve the above object, in this ultraviolet sterilization method, in the ultraviolet sterilization method of irradiating the indoor surface with an ultraviolet lamp, the sterilization target is Escherichia coli , and the ultraviolet intensity is 0.008 to 0.170 mW / cm. 2 and the irradiation time is 3 minutes 13 seconds to 37 seconds.
Furthermore, in the ultraviolet sterilization method of irradiating the interior surface with ultraviolet rays by an ultraviolet lamp, the sterilization target is Salmonella, the ultraviolet intensity is 0.008 to 0.170 mW / cm 2 , and the irradiation time is 3 minutes 12 seconds to 21 seconds. It is characterized by that.
従って、この紫外線殺菌方法によれば、紫外線ランプの設置本数を多くすることなく、ごく低い紫外線強度で、殺菌対象である細菌の殺菌を行うことができて、殺菌設備の導入時におけるコストの低減を図ることができる。また、殺菌効果の評価により、同じ紫外線照射量（紫外線強度×照射時間）においては、（紫外線強度：大×照射時間：短）の場合よりも、（紫外線強度：小×照射時間：長）の場合の方が、殺菌効果が高いことが判明した。よって、ごく低い紫外線強度を用いた場合でも照射時間を短縮することができて、消費電力の削減及びランプ寿命の延長を図ることができ、運用コストも低減することができる。 Therefore, according to this ultraviolet sterilization method, the bacteria to be sterilized can be sterilized with a very low ultraviolet intensity without increasing the number of installed ultraviolet lamps, and the cost when introducing the sterilization equipment can be reduced. Can be achieved. Moreover, by the evaluation of the bactericidal effect, at the same ultraviolet ray irradiation amount (ultraviolet ray intensity × irradiation time), (ultraviolet ray intensity: small × irradiation time: long) than in the case of (ultraviolet ray intensity: large × irradiation time: short). The case was found to have a higher bactericidal effect. Therefore, even when a very low ultraviolet intensity is used, the irradiation time can be shortened, the power consumption can be reduced, the lamp life can be extended, and the operation cost can be reduced.
また、別の紫外線殺菌方法では、紫外線ランプによって室内面に紫外線を照射する紫外線殺菌方法において、殺菌対象が芽胞セレウス菌であって、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ２ で、照射時間を４分３５秒〜１分２９秒とすることを特徴としている。
更に、紫外線ランプによって室内面に紫外線を照射する紫外線殺菌方法において、殺菌対象が芽胞枯草菌であって、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ ２ で、照射時間を５分１０秒〜３分２６秒とすることを特徴としている。
Moreover, in another ultraviolet sterilization method, in the ultraviolet sterilization method of irradiating the interior surface with ultraviolet rays using an ultraviolet lamp, the sterilization target is spores of Bacillus cereus , and the ultraviolet intensity is 0.030 to 0.170 mW / cm 2 . The time is 4 minutes 35 seconds to 1 minute 29 seconds.
Furthermore, in the ultraviolet sterilization method of irradiating the indoor surface with ultraviolet rays by an ultraviolet lamp, the sterilization target is spores Bacillus subtilis, the ultraviolet intensity is 0.030 to 0.170 mW / cm 2 , and the irradiation time is 5 minutes 10 seconds to It is characterized by 3 minutes 26 seconds .
さらに、別の紫外線殺菌方法では、紫外線ランプによって室内面に紫外線を照射する紫外線殺菌方法において、殺菌対象がコウジカビであって、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ２ で、照射時間を９分４３秒〜２８０秒とすることを特徴とすることを特徴としている。
更に、紫外線ランプによって室内面に紫外線を照射する紫外線殺菌方法において、殺菌対象がアオカビであって、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ ２ で、照射時
間を８分１０秒〜２８０秒とすることを特徴としている。
また、紫外線ランプによって室内面に紫外線を照射する紫外線殺菌方法において、殺菌対象がクラドスポリウム属の菌であって、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ ２ で、照射時間を２９分２０秒〜５分１１秒とすることを特徴としている。
Further, in another ultraviolet sterilization method, in the ultraviolet sterilization method of irradiating ultraviolet rays to the interior surface by an ultraviolet lamp, sterilized it is an Aspergillus, a UV intensity 0.030~0.170mW / cm 2, the irradiation time It is characterized by being 9 minutes 43 seconds- 280 seconds .
Furthermore, in the ultraviolet sterilization method of irradiating the indoor surface with ultraviolet rays by an ultraviolet lamp, the sterilization target is blue mold, the ultraviolet intensity is 0.030 to 0.170 mW / cm 2 ,
The interval is set to 8 minutes 10 seconds to 280 seconds.
Moreover, in the ultraviolet sterilization method of irradiating an indoor surface with ultraviolet rays by an ultraviolet lamp, the sterilization target is bacteria of the genus Cladosporium, the ultraviolet intensity is 0.030 to 0.170 mW / cm 2 , and the irradiation time is 29 minutes. It is characterized by being 20 seconds to 5 minutes and 11 seconds .
前記の紫外線殺菌方法によれば、紫外線ランプの設置本数を減らし、ごく低い紫外線強度で細菌やカビの殺菌を行うことができて、殺菌設備の導入時におけるコスト低減を図ることができる。さらに、ごく低い紫外線強度を用いた場合でも照射時間を短縮することができ、消費電力の削減及びランプ寿命の延長を図ることができ、運用コストも低減することができるという効果を発揮する。 According to the ultraviolet sterilization method, the number of ultraviolet lamps can be reduced, bacteria and molds can be sterilized with a very low ultraviolet intensity, and the cost can be reduced when the sterilization equipment is introduced. Furthermore, even when a very low UV intensity is used, the irradiation time can be shortened, the power consumption and the lamp life can be extended, and the operational cost can be reduced.
（第１実施形態）
以下、本発明の紫外線殺菌方法を具体化した第１実施形態について説明する。
第１実施形態における紫外線殺菌方法は、紫外線ランプによって室内の壁面，床面，天井面、あるいは各種装置の表面、空調装置の送風ダクト内面等の各種部位に紫外線を照射する方法である。この場合、殺菌対象は細菌であって、紫外線強度は０．００８〜０．１７０ｍＷ／ｃｍ２に設定される。殺菌対象としての室内面は、壁面、床面、天井面等である。細菌としては、例えば大腸菌、サルモネラ菌等が挙げられる。紫外線は、波長が１０〜４００ｎｍの範囲の電磁波であり、殺菌消毒作用等の化学的作用を発揮する。この紫外線は、紫外線ランプにより殺菌対象から所定の距離をおいて照射される。
(First embodiment)
Hereinafter, a first embodiment embodying the ultraviolet sterilization method of the present invention will be described.
The ultraviolet sterilization method in 1st Embodiment is a method of irradiating an ultraviolet-ray to various parts, such as an indoor wall surface, a floor surface, a ceiling surface, the surface of various apparatuses, the air blower duct inner surface of an air conditioner, with an ultraviolet lamp. In this case, the sterilization target is bacteria, and the ultraviolet intensity is set to 0.008 to 0.170 mW / cm 2 . The indoor surface as a sterilization target is a wall surface, a floor surface, a ceiling surface, or the like. Examples of bacteria include Escherichia coli and Salmonella. Ultraviolet rays are electromagnetic waves having a wavelength in the range of 10 to 400 nm, and exhibit chemical actions such as sterilization and disinfection. This ultraviolet ray is irradiated at a predetermined distance from the object to be sterilized by an ultraviolet lamp.
紫外線強度は前記の範囲に設定されるが、その紫外線強度が０．００８ｍＷ／ｃｍ２を下回る場合には、紫外線強度が不足し、長時間に亘って紫外線を照射しなければならなくなる。その一方、紫外線強度が０．１７０ｍＷ／ｃｍ２を上回る場合には、合成樹脂等の劣化を促進したり、人の目に悪影響を及ぼしたりするおそれがある。 The ultraviolet intensity is set in the above range, but when the ultraviolet intensity is less than 0.008 mW / cm 2 , the ultraviolet intensity is insufficient, and it is necessary to irradiate ultraviolet rays for a long time. On the other hand, when the ultraviolet intensity exceeds 0.170 mW / cm 2 , deterioration of the synthetic resin or the like may be promoted or the human eyes may be adversely affected.
前記殺菌対象が細菌の芽胞形成菌である場合には、紫外線強度が０．０３０〜０．１７０ｍＷ／ｃｍ２に設定される。芽胞形成菌としては、芽胞セレウス菌、芽胞枯草菌、ウェルシュ菌等が挙げられる。この芽胞形成菌は細菌の一種ではあるが、前記大腸菌、サルモネラ菌等に比べて耐紫外線性が若干高くなっている。このため、紫外線強度の範囲の下限を０．０３０ｍＷ／ｃｍ２とする必要がある。紫外線強度が０．０３０ｍＷ／ｃｍ２に満たない場合には、芽胞形成菌を例えば９９％殺菌することが難しくなる。 When the sterilization target is a spore-forming bacterium, the ultraviolet intensity is set to 0.030 to 0.170 mW / cm 2 . Examples of spore-forming bacteria include spores of Bacillus cereus, Bacillus subtilis, and Clostridium perfringens. Although this spore-forming bacterium is a kind of bacteria, it has slightly higher ultraviolet resistance than the aforementioned Escherichia coli, Salmonella, and the like. For this reason, the lower limit of the range of ultraviolet intensity needs to be 0.030 mW / cm 2 . When the ultraviolet intensity is less than 0.030 mW / cm 2 , it becomes difficult to sterilize, for example, 99% of the spore-forming bacteria.
次に、第１実施形態の紫外線殺菌方法について作用を説明する。
さて、室内において大腸菌等の細菌や芽胞セレウス菌等の芽胞形成菌を殺菌する場合には、室内面に対して紫外線を照射する。このとき、殺菌対象が大腸菌等の細菌である場合には紫外線強度を０．００８〜０．１７０ｍＷ／ｃｍ２に設定し、殺菌対象が芽胞セレウス菌等の芽胞形成菌である場合には紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ２に設定する。すると、前記範囲の紫外線強度を有する紫外線が細菌の細胞内に吸収されて化学変化を起こし、その細菌は新陳代謝が阻害されるとともに、増殖能力を失って死滅するものと推測される。また、その紫外線は細菌の細胞に直接働いて殺菌作用を示すことから、耐性菌の発生が抑制される。
Next, an effect | action is demonstrated about the ultraviolet sterilization method of 1st Embodiment.
Now, in order to sterilize bacteria such as Escherichia coli and spore-forming bacteria such as Bacillus cereus in the room, the interior surface is irradiated with ultraviolet rays. At this time, when the sterilization target is a bacterium such as Escherichia coli, the ultraviolet intensity is set to 0.008 to 0.170 mW / cm 2 , and when the sterilization target is a spore-forming bacterium such as spore Bacillus cereus, the ultraviolet intensity is set. Is set to 0.030 to 0.170 mW / cm 2 . Then, it is presumed that ultraviolet rays having ultraviolet intensity in the above-mentioned range are absorbed into bacterial cells to cause a chemical change, and the bacteria are inhibited from metabolism and lose their growth ability and die. Moreover, since the ultraviolet rays work directly on bacterial cells and exhibit a bactericidal action, the generation of resistant bacteria is suppressed.
従って、この第１実施形態によれば、以下のような効果を得ることができる。
（１）この紫外線殺菌方法においては、紫外線ランプによって室内面に紫外線を照射する殺菌方法において、殺菌対象が大腸菌、サルモネラ菌等の細菌である場合には、紫外線強度を０．００８〜０．１７０ｍＷ／ｃｍ２としている。また、殺菌対象が芽胞セレウス菌、芽胞枯草菌等の芽胞形成菌である場合には、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ２としている。
Therefore, according to the first embodiment, the following effects can be obtained.
(1) In this ultraviolet sterilization method, when the object to be sterilized is bacteria such as Escherichia coli and Salmonella in the sterilization method of irradiating the indoor surface with an ultraviolet lamp, the ultraviolet intensity is 0.008 to 0.170 mW / cm 2 . Moreover, when the sterilization target is a spore-forming bacterium such as a spore Bacillus cereus or a spore Bacillus subtilis, the ultraviolet intensity is set to 0.030 to 0.170 mW / cm 2 .
よって、この紫外線殺菌方法によれば、紫外線ランプの設置本数を多くすることなく、ごく低い紫外線強度で、殺菌対象である細菌の殺菌を行うことができて、殺菌設備の導入時におけるコストの低減を図ることができる。また、ごく低い紫外線強度を用いた場合でも照射時間を短縮することができて、消費電力の削減及びランプ寿命の延長を図ることができ、運用コストも低減することができる。
（第２実施形態）
次に、本発明の紫外線殺菌方法の第２実施形態を、前記第１実施形態と異なる部分を中心に説明する。
Therefore, according to this ultraviolet sterilization method, it is possible to sterilize the bacteria to be sterilized with a very low ultraviolet intensity without increasing the number of installed ultraviolet lamps, and to reduce the cost when introducing the sterilization equipment. Can be achieved. Further, even when an extremely low ultraviolet intensity is used, the irradiation time can be shortened, power consumption and lamp life can be extended, and the operation cost can be reduced.
(Second Embodiment)
Next, a second embodiment of the ultraviolet sterilization method of the present invention will be described with a focus on differences from the first embodiment.
さて、この第２実施形態の紫外線殺菌方法は、紫外線ランプによって室内面に紫外線を照射する紫外線殺菌方法であって、殺菌対象がカビであり、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ２とするとともに、紫外線照射時間を３０分〜２８０秒とするものである。殺菌対象のカビとしては、コウジカビ、アオカビ、クロカビ等が挙げられる。 The ultraviolet sterilization method according to the second embodiment is an ultraviolet sterilization method in which the interior surface is irradiated with ultraviolet rays by an ultraviolet lamp, the sterilization target is mold, and the ultraviolet intensity is 0.030 to 0.170 mW / cm 2. In addition, the ultraviolet irradiation time is set to 30 minutes to 280 seconds. Examples of mold to be sterilized include Aspergillus, mold, black mold and the like.
紫外線強度が０．０３０ｍＷ／ｃｍ２に満たない場合には、紫外線強度が不十分で、紫外線の照射時間を必要以上に長くしなければならなくなる。一方、紫外線強度が０．１７０ｍＷ／ｃｍ２を超える場合には、室内面を構成する材料の劣化を促進したり、人の目に悪影響を及ぼしたりするおそれがある。また、紫外線照射時間が２８０秒より短い場合、紫外線の照射量が不足し、カビに対する所望の殺菌効果が得られなくなる。一方、紫外線照射時間が３０分より長い場合、紫外線照射量が過剰となり、カビに対する殺菌効果以外に紫外線が照射される対象に弊害を及ぼすおそれがある。 When the ultraviolet intensity is less than 0.030 mW / cm 2 , the ultraviolet intensity is insufficient, and the irradiation time of the ultraviolet ray must be made longer than necessary. On the other hand, when the ultraviolet intensity exceeds 0.170 mW / cm 2 , there is a risk of accelerating deterioration of the material constituting the indoor surface or adversely affecting human eyes. Moreover, when the ultraviolet irradiation time is shorter than 280 seconds, the irradiation amount of ultraviolet rays is insufficient, and a desired sterilizing effect against mold cannot be obtained. On the other hand, when the ultraviolet irradiation time is longer than 30 minutes, the ultraviolet irradiation amount becomes excessive, and there is a possibility of adversely affecting an object irradiated with ultraviolet rays in addition to the bactericidal effect against mold.
従って、この第２実施形態によれば、以下のような効果を得ることができる。
（２）この紫外線殺菌方法においては、紫外線ランプによって室内面に紫外線を照射する殺菌方法において、殺菌対象がコウジカビ、アオカビ等のカビである場合に、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ２ とするとともに、紫外線照射時間を３０分〜２８０秒としている。
Therefore, according to the second embodiment, the following effects can be obtained.
(2) In this ultraviolet sterilization method, when the object to be sterilized is mold such as Aspergillus or blue mold in the sterilization method of irradiating the interior surface with an ultraviolet lamp, the ultraviolet intensity is 0.030 to 0.170 mW / cm. 2 and the ultraviolet irradiation time is 30 minutes to 280 seconds.
よって、殺菌対象がカビである場合においても、前述した第１実施形態の殺菌対象が細菌である場合と同様に、紫外線ランプの設置本数を多くすることなく、ごく低い紫外線強度で、殺菌対象である細菌の殺菌を行うことができて、殺菌設備の導入時におけるコストの低減を図ることができる。また、ごく低い紫外線強度を用いた場合でも照射時間を短縮することができて、消費電力の削減及びランプ寿命の延長を図ることができ、運用コストも低減することができる。 Therefore, even when the sterilization target is mold, as in the case where the sterilization target of the first embodiment is bacteria, the sterilization target can be sterilized with a very low UV intensity without increasing the number of UV lamps installed. A certain bacterium can be sterilized, and the cost at the time of introduction of the sterilization equipment can be reduced. Further, even when an extremely low ultraviolet intensity is used, the irradiation time can be shortened, power consumption and lamp life can be extended, and the operation cost can be reduced.
以下に、実施例を挙げて前記実施形態をさらに具体的に説明する。
（実施例１〜４）
殺菌対象が細菌として、実施例１では大腸菌、実施例２ではサルモネラ菌、実施例３では芽胞セレウス菌である場合及び実施例４では芽胞枯草菌である場合について、ごく低い紫外線強度で殺菌を行ったときに、どの程度の殺菌効果を発揮できるかの殺菌試験を行った。この場合、各細菌を液体培地又は斜面培地で培養し、定常状態にまで培養後、遠心分離により細菌を集菌し、生理食塩水で洗浄したものを試験用菌液とした。そして、プレート培地（細菌はＳＣＤ培地、カビはＰＤＡ培地）１枚につき、プレートあたりの菌添加量が１０３〜１０５となるように菌液を２００μｌ塗布した。紫外線照射しないものをコントロールサンプルとし、５段階の照射時間で照射したサンプルを試験サンプルとした。
Hereinafter, the embodiment will be described more specifically with reference to examples.
(Examples 1-4)
The bacteria to be sterilized were E. coli in Example 1, Salmonella in Example 2, Spore Bacillus in Example 3, and Spore Bacillus in Example 4, and sterilized with very low UV intensity. Occasionally, a sterilization test was performed to see how much sterilization effect can be exhibited. In this case, each bacterium was cultured in a liquid medium or a slant medium, cultured to a steady state, collected by centrifugation, and washed with physiological saline to obtain a test bacterial solution. Then, 200 μl of the bacterial solution was applied so that the amount of added bacteria per plate was 10 3 to 10 5 per plate medium (bacteria were SCD medium and mold was PDA medium). A sample that was not irradiated with ultraviolet rays was used as a control sample, and a sample that was irradiated with five irradiation times was used as a test sample.
この試験サンプルに、０．１７０ｍＷ／ｃｍ２、０．０８５ｍＷ／ｃｍ２、０．０３０ｍＷ／ｃｍ２、及び０．００８ｍＷ／ｃｍ２の４種類又は３種類の紫外線強度条件下で、一定時間紫外線を照射した。紫外線照射後、３２℃のインキュベータで２日間培養し、生存菌数（コロニー数）を測定した。そして、紫外線の照射前の菌数と比較評価した。これによって、表３に示すような殺菌試験結果が得られた。なお、表３は殺菌対象が芽胞枯草菌である場合の殺菌試験結果を示すものであるが、他種類の細菌についても同様の殺菌試験結果が得られた。 This test sample, 0.170mW / cm 2, 0.085mW / cm 2, 0.030mW / cm 2, and at four or three UV intensity conditions of 0.008mW / cm 2, the predetermined time UV Irradiated. After ultraviolet irradiation, the cells were cultured in an incubator at 32 ° C. for 2 days, and the number of viable bacteria (colony number) was measured. And it compared with the number of bacteria before irradiation of an ultraviolet-ray, and evaluated. Thereby, the sterilization test results as shown in Table 3 were obtained. Table 3 shows the sterilization test results when the sterilization target is Spore Bacillus subtilis, but similar sterilization test results were obtained for other types of bacteria.
この殺菌試験結果に基づいて、紫外線の照射量０（Ｊ／ｍ２）の培地で培養されたコロニー数に対する、紫外線照射後に生育したコロニー数との比率で、生存率を求めた。１つのプレートにおける紫外線照射量と生存菌数との関係をグラフ化すると、図１に示すようになった。また、同プレートの紫外線照射量と生存率との関係を近似的にグラフ化すると、図２に示すようになった。この図２に示す直線から、初期の菌数を９９．９％殺菌する（１／１０００以下にする）ために必要な紫外線照射量を求めたところ、表１及び表２に示すような評価結果が得られた。 Based on the results of this bactericidal test, the survival rate was determined based on the ratio of the number of colonies grown after ultraviolet irradiation to the number of colonies cultured in a medium with an ultraviolet irradiation amount of 0 (J / m 2 ). A graph showing the relationship between the amount of ultraviolet irradiation and the number of viable bacteria on one plate is shown in FIG. Further, when the relationship between the ultraviolet irradiation amount and the survival rate of the plate is approximately graphed, it is as shown in FIG. From the straight line shown in FIG. 2, when the amount of ultraviolet irradiation necessary for sterilizing the initial number of bacteria by 99.9% (less than 1/1000) was determined, the evaluation results as shown in Table 1 and Table 2 were obtained. was gotten.
ることが明らかになった。さらに、殺菌対象が殺菌しにくい芽胞枯草菌である場合、紫外線強度が０．０３０ｍＷ／ｃｍ２と低い値であっても、５分半以内に初期の菌数の１／１０００以下まで殺菌できることが判明した。
（実施例５，実施例６及び実施例７）
殺菌対象がコウジカビである実施例５，アオカビである実施例６，クロカビである実施例７について、前記実施例１〜４の場合と同様に、０．１７０ｍＷ／ｃｍ２、０．０８５ｍＷ／ｃｍ２ 及び０．０３０ｍＷ／ｃｍ ２ の３種類のごく低い紫外線強度条件下で殺菌試験を行った。なお、この場合、紫外線照射後の培養期間が、２５℃のインキュベータで５日間であることが表１〜３の場合と異なる。それにより、表４に示すような評価結果が得られた。その結果、殺菌対象がコウジカビである場合、紫外線強度が０．０３０ｍＷ／ｃｍ２と低い値であっても、約１０分以内に初期の菌数の１／１０００以下まで殺菌できることが判明した。
(Example 5, Example 6 and Example 7)
Example 5 sterilized is aspergillus, Example 6 is a Penicillium, for Example 7 is Aspergillus niger, as in the case of Examples 1~4, 0.170mW / cm 2, 0.085mW / cm 2 and it was sterilized test in three very low UV intensity conditions of 0.030mW / cm 2. In this case, the culture period after ultraviolet irradiation is 5 days in an incubator at 25 ° C., which is different from the cases shown in Tables 1 to 3. Thereby, the evaluation results as shown in Table 4 were obtained. As a result, it was found that even when the object of sterilization is Aspergillus, even if the ultraviolet intensity is as low as 0.030 mW / cm 2, it can be sterilized to about 1/1000 or less of the initial number of bacteria within about 10 minutes.
また、殺菌対象がアオカビである場合、紫外線強度が０．０３０ｍＷ／ｃｍ２と低い値であっても、８分半以内に初期の菌数の１／１０００以下まで殺菌できることが判明した。さらに、殺菌対象がクロカビ（「クラドスポリウム属の菌」）である場合、紫外線強度が０．０３０ｍＷ／ｃｍ２と低い値であっても、２９分半以内に初期の菌数の１／１０００以下まで殺菌できることが判明した。 In addition, when the object of sterilization is blue mold, it has been found that even if the ultraviolet intensity is as low as 0.030 mW / cm 2, it can be sterilized to 1/1000 or less of the initial number of bacteria within 8 minutes and a half . Furthermore, when the object of sterilization is black mold (“Cladosporum spp.”) , Even if the UV intensity is as low as 0.030 mW / cm 2 , 1/1000 of the initial number of bacteria within 29 minutes and a half. It was found that the following can be sterilized.
このことから、殺菌対象がカビである場合には、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ２ とするとともに、紫外線照射時間を３０分〜２８０秒として殺菌を行えば、十分な殺菌効果が得られることが判明した。 From this, when the object of sterilization is mold, sufficient sterilization effect can be obtained if the sterilization is performed with an ultraviolet intensity of 0.030 to 0.170 mW / cm 2 and an ultraviolet irradiation time of 30 minutes to 280 seconds. Was found to be obtained.
Claims (7)
殺菌対象が大腸菌であって、紫外線強度を０．００８〜０．１７０ｍＷ／ｃｍ２ で、照射時間を３分１３秒〜３７秒とすることを特徴とする紫外線殺菌方法。 In the ultraviolet sterilization method of irradiating the indoor surface with ultraviolet rays by an ultraviolet lamp,
An ultraviolet sterilization method characterized in that the sterilization target is Escherichia coli , the ultraviolet intensity is 0.008 to 0.170 mW / cm 2 , and the irradiation time is 3 minutes 13 seconds to 37 seconds .
殺菌対象がサルモネラ菌であって、紫外線強度を０．００８〜０．１７０ｍＷ／ｃｍ ２ で、照射時間を３分１２秒〜２１秒とすることを特徴とする紫外線殺菌方法。 In the ultraviolet sterilization method of irradiating the indoor surface with ultraviolet rays by an ultraviolet lamp,
An ultraviolet sterilization method, wherein the sterilization target is Salmonella , the ultraviolet intensity is 0.008 to 0.170 mW / cm 2 , and the irradiation time is 3 minutes 12 seconds to 21 seconds .
殺菌対象が芽胞セレウス菌であって、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ２ で、照射時間を４分３５秒〜１分２９秒とすることを特徴とする紫外線殺菌方法。 In the ultraviolet sterilization method of irradiating the indoor surface with ultraviolet rays by an ultraviolet lamp,
An ultraviolet sterilization method characterized in that an object to be sterilized is Spore cerevisiae, an ultraviolet intensity is 0.030 to 0.170 mW / cm 2 , and an irradiation time is 4 minutes 35 seconds to 1 minute 29 seconds .
殺菌対象が芽胞枯草菌であって、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ ２ で、照射時間を５分１０秒〜３分２６秒とすることを特徴とする紫外線殺菌方法。 In the ultraviolet sterilization method of irradiating the indoor surface with ultraviolet rays by an ultraviolet lamp,
An ultraviolet sterilization method characterized in that an object to be sterilized is spores Bacillus subtilis , the ultraviolet intensity is 0.030 to 0.170 mW / cm 2 , and the irradiation time is 5 minutes 10 seconds to 3 minutes 26 seconds .
殺菌対象がコウジカビであって、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ２ で、照射時間を９分４３秒〜２８０秒とすることを特徴とする紫外線殺菌方法。 In the ultraviolet sterilization method of irradiating the indoor surface with ultraviolet rays by an ultraviolet lamp,
Sterilized is an Aspergillus, a UV intensity 0.030~0.170mW / cm 2, UV sterilization method which is characterized in that the irradiation time of 9 minutes 43 seconds to 280 seconds.
殺菌対象がアオカビであって、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ ２ で、照射時間を８分１０秒〜２８０秒とすることを特徴とする紫外線殺菌方法。 In the ultraviolet sterilization method of irradiating the indoor surface with ultraviolet rays by an ultraviolet lamp,
An ultraviolet sterilization method characterized in that the object of sterilization is blue mold , the ultraviolet intensity is 0.030 to 0.170 mW / cm 2 , and the irradiation time is 8 minutes 10 seconds to 280 seconds .
殺菌対象がクラドスポリウム属の菌であって、紫外線強度を０．０３０〜０．１７０ｍＷ／ｃｍ ２ で、照射時間を２９分２０秒〜５分１１秒とすることを特徴とする紫外線殺菌方法。 In the ultraviolet sterilization method of irradiating the indoor surface with ultraviolet rays by an ultraviolet lamp,
The sterilization target is a bacterium belonging to the genus Cladosporium, the ultraviolet intensity is 0.030 to 0.170 mW / cm 2 , and the irradiation time is 29 minutes 20 seconds to 5 minutes 11 seconds, .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2013007561A JP6135141B2 (en) | 2013-01-18 | 2013-01-18 | UV sterilization method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2013007561A JP6135141B2 (en) | 2013-01-18 | 2013-01-18 | UV sterilization method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2014136113A JP2014136113A (en) | 2014-07-28 |
| JP6135141B2 true JP6135141B2 (en) | 2017-05-31 |
Family
ID=51413966
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013007561A Active JP6135141B2 (en) | 2013-01-18 | 2013-01-18 | UV sterilization method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6135141B2 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7261994B2 (en) * | 2016-08-10 | 2023-04-21 | パナソニックＩｐマネジメント株式会社 | Antibacterial method and antibacterial device |
| US10898600B2 (en) | 2016-08-10 | 2021-01-26 | Panasonic Intellectual Property Management Co., Ltd. | Disinfecting method and disinfecting apparatus |
| KR20180075123A (en) | 2016-12-26 | 2018-07-04 | 엘지전자 주식회사 | Control method for sterilization of water purifying apparatus |
| EP4109493A4 (en) * | 2020-02-18 | 2023-11-08 | Ushio Denki Kabushiki Kaisha | Bacteriostatic method |
| JP7099500B2 (en) * | 2020-08-25 | 2022-07-12 | ウシオ電機株式会社 | Inactivating device and inactivating method |
| CN113842481B (en) * | 2021-09-29 | 2023-03-17 | 华南农业大学 | Ultraviolet sterilization synergist lime essential oil and application thereof |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH058819A (en) * | 1991-01-24 | 1993-01-19 | Yokohama Rubber Co Ltd:The | Food conveying device |
| JPH06181969A (en) * | 1992-12-21 | 1994-07-05 | Nishimura Seisakusho:Kk | Slitter |
| JPH078541A (en) * | 1993-06-28 | 1995-01-13 | Fujita Corp | Method for sterilizing building |
| JPH11169440A (en) * | 1997-12-15 | 1999-06-29 | Lion Corp | Method and device for sterilizing storage tank for storing liquid raw material |
| JP2001245960A (en) * | 2000-03-08 | 2001-09-11 | Takenaka Komuten Co Ltd | Accommodation body, sterile room using it, and sterilizing method |
| JP2002080017A (en) * | 2000-06-26 | 2002-03-19 | Hoshin Kagaku Sangyosho:Kk | Sterilization device |
| JP2003225288A (en) * | 2001-11-29 | 2003-08-12 | Iida Kensetsu:Kk | Vessel for sterilization |
| US20030223904A1 (en) * | 2002-06-04 | 2003-12-04 | Lucas Lakhdar Bacha | UV bulk mail irradiation system |
| JP2005168858A (en) * | 2003-12-12 | 2005-06-30 | Keishin Aoki | Portable mildew-proof device |
| JP2007327192A (en) * | 2006-06-06 | 2007-12-20 | Paruka:Kk | Mildewproof structure |
| KR101685456B1 (en) * | 2011-04-15 | 2016-12-12 | 사무엘 리차드 트라파니 | Room sterilization method and system |
-
2013
- 2013-01-18 JP JP2013007561A patent/JP6135141B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2014136113A (en) | 2014-07-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6135141B2 (en) | UV sterilization method | |
| Nerandzic et al. | Sorting through the wealth of options: comparative evaluation of two ultraviolet disinfection systems | |
| Menetrez et al. | The effectiveness of UV irradiation on vegetative bacteria and fungi surface contamination | |
| JP5435619B2 (en) | Surface sterilizer | |
| WO2010087831A8 (en) | Improved method and apparatus for producing a high level of disinfection in air and surfaces | |
| US20130202479A1 (en) | Plasma sterilizer, plasma sterilization system, and plasma sterilization method | |
| JP6042369B2 (en) | Ultraviolet laser sterilization system | |
| CN203861628U (en) | All-intelligent dynamic air sterilization and purification machine | |
| CN105465978A (en) | Domestic bathroom intelligent purification system | |
| FI20175639A1 (en) | System and method for controlling growth of microorganisms | |
| KR101990846B1 (en) | System and Method for Monitoring Air Contamination by Using Ultraviolet Air Sterilizer | |
| WO2019061035A1 (en) | Ultraviolet system for disinfection and ultraviolet disinfection method | |
| Holtkamp et al. | Ultraviolet C (UVC) Standards and Best Practices for the Swine Industry | |
| WO2020052249A1 (en) | Cleaning operation area microorganism control system and use method therefor | |
| CN104503273A (en) | Toilet disinfection control system | |
| Shintani et al. | Sterilization efficiency of the photocatalyst against environmental microorganisms in a health care facility | |
| JPH078541A (en) | Method for sterilizing building | |
| CN106277114A (en) | Circulating water disinfection equipment | |
| Mally et al. | Simulation based design of an UVC-LED emitter for disinfection of high-touch environmental surfaces | |
| US20210275705A1 (en) | Combination ultra-violet a (uva) and ultra-violet c (uvc) system for reduction and inhibition of growth of pathogens | |
| Arkusz et al. | Shedding light on the problem: Influence of the radiator power, source-sample distance, and exposure time on the performance of UV-C lamps in laboratory and real-world conditions | |
| Kulkarni et al. | Optical filter enabled continuous disinfection of hospital rooms using multi-sensor feedback aided light source | |
| RU124567U1 (en) | FOOD PROCESSING DEVICE | |
| CN208617813U (en) | Disinfector is used in a kind of production of bamboo wine | |
| KR102558645B1 (en) | Management control system including IoT-based air sterilizers |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20151209 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20161124 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20161206 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20170206 |
|
| A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20170220 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20170328 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20170410 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6135141 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |