JP6092726B2 - Anaerobic culture device - Google Patents
Anaerobic culture device Download PDFInfo
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- JP6092726B2 JP6092726B2 JP2013143081A JP2013143081A JP6092726B2 JP 6092726 B2 JP6092726 B2 JP 6092726B2 JP 2013143081 A JP2013143081 A JP 2013143081A JP 2013143081 A JP2013143081 A JP 2013143081A JP 6092726 B2 JP6092726 B2 JP 6092726B2
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- 239000001257 hydrogen Substances 0.000 claims description 28
- 229910052739 hydrogen Inorganic materials 0.000 claims description 28
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 27
- 238000001514 detection method Methods 0.000 claims description 18
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 239000003054 catalyst Substances 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 230000007423 decrease Effects 0.000 claims description 7
- 230000007613 environmental effect Effects 0.000 claims description 2
- 238000000638 solvent extraction Methods 0.000 claims 1
- 238000005192 partition Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
本発明は、嫌気培養装置に関する。 The present invention relates to an anaerobic culture apparatus.
微生物などを嫌気環境で培養するための嫌気培養装置がある。嫌気培養装置の1つとして、内部空間を外部空間から仕切る嫌気性チャンバーと、内部空間に水素を供給するユニットとを備えるものがある。内部空間には、触媒が配置されていて、この触媒が水素と酸素との反応を促進させることによって内部空間における酸素の濃度が低下する。 There is an anaerobic culture apparatus for culturing microorganisms in an anaerobic environment. One anaerobic culture apparatus includes an anaerobic chamber that partitions an internal space from an external space, and a unit that supplies hydrogen to the internal space. A catalyst is disposed in the internal space, and this catalyst promotes the reaction between hydrogen and oxygen, so that the concentration of oxygen in the internal space decreases.
上記のような嫌気培養装置において、嫌気性チャンバーが破損し、内部空間から外部空間に水素が漏れ続けると、危険である。そこで、嫌気培養装置が配置された部屋に水素センサを配置して、これによって水素を検知することが考えられる。しかしながら、例えば、当該部屋に空気の流れがあると、嫌気性チャンバーからの水素の漏れを確実に検知することが難しい。 In the anaerobic culture apparatus as described above, it is dangerous if the anaerobic chamber is damaged and hydrogen continues to leak from the internal space to the external space. Therefore, it is conceivable that a hydrogen sensor is arranged in a room where an anaerobic culture apparatus is arranged to detect hydrogen. However, for example, if there is an air flow in the room, it is difficult to reliably detect hydrogen leakage from the anaerobic chamber.
本発明は、嫌気性チャンバーからの水素の漏れをより確実に検知することを目的とする。 An object of the present invention is to more reliably detect hydrogen leakage from an anaerobic chamber.
本発明は、嫌気培養装置に係り、前記嫌気培養装置は、可撓性部材によって構成され内部空間を外部空間から仕切る嫌気性チャンバーと、前記内部空間に配置された触媒が水素と酸素との反応を促進させることによって前記内部空間における酸素の濃度が低下するように、前記内部空間に水素を供給する環境制御部と、前記内部空間から前記外部空間への水素の漏れを前記内部空間の圧力の低下による前記嫌気性チャンバーの変形に基づいて検知する検知部と、前記検知部による水素の漏れの検知に応じて警報を発する警報部と、を備える。 The present invention relates to an anaerobic culture apparatus, wherein the anaerobic culture apparatus is constituted by a flexible member and partitions an internal space from an external space, and a catalyst disposed in the internal space reacts with hydrogen and oxygen. The environmental control unit for supplying hydrogen to the internal space and the leakage of hydrogen from the internal space to the external space to reduce the pressure of the internal space. A detection unit that detects based on deformation of the anaerobic chamber due to a decrease, and an alarm unit that issues an alarm in response to detection of hydrogen leakage by the detection unit.
本発明によれば、嫌気性チャンバーからの水素の漏れをより確実に検知する。 According to the present invention, leakage of hydrogen from the anaerobic chamber is more reliably detected.
以下、添付図面を参照しながら本発明をその例示的な実施形態の嫌気培養装置100を通して説明する。 Hereinafter, the present invention will be described through an anaerobic culture apparatus 100 of an exemplary embodiment with reference to the accompanying drawings.
嫌気培養装置100は、嫌気性チャンバー10と、環境制御部30と、検知部70と、警報部80とを備えている。嫌気性チャンバー10は、可撓性部材によって構成され、内部空間ISを外部空間OSから仕切る。可撓性部材は、例えば、塩化ビニールで形成されうる。内部空間ISには、水素と酸素との反応を促進させるための触媒20が配置される。触媒20は、例えば、内部空間ISに配置された棚60に置かれうる。 The anaerobic culture apparatus 100 includes an anaerobic chamber 10, an environment control unit 30, a detection unit 70, and an alarm unit 80. The anaerobic chamber 10 is configured by a flexible member, and partitions the internal space IS from the external space OS. The flexible member can be formed of, for example, vinyl chloride. A catalyst 20 for accelerating the reaction between hydrogen and oxygen is disposed in the internal space IS. The catalyst 20 can be placed on, for example, a shelf 60 disposed in the internal space IS.
環境制御部30は、内部空間ISに配置された触媒20が水素と酸素との反応を促進させることによって内部空間ISにおける酸素の濃度が低下するように、内部空間ISに水素を供給する。一例において、環境制御部30は、まず、内部空間ISの空気を窒素ガスで置換し、その後に、触媒20の作用により内部空間ISにおける酸素の濃度が低下するように、内部空間ISに水素を供給する。 The environment control unit 30 supplies hydrogen to the internal space IS such that the catalyst 20 disposed in the internal space IS promotes the reaction between hydrogen and oxygen, so that the oxygen concentration in the internal space IS decreases. In one example, the environment control unit 30 first replaces the air in the internal space IS with nitrogen gas, and then supplies hydrogen to the internal space IS so that the concentration of oxygen in the internal space IS decreases due to the action of the catalyst 20. Supply.
嫌気性チャンバー10が損傷を受けると、嫌気性チャンバー10から水素が漏れうる。典型的な例としては、嫌気性チャンバー10には操作用のグローブが設けられていて、そのグローブの先端等が摩耗等によって薄くなって、そこに穴が開きうる。このような穴を通して比較的多くの水素が内部空間ISから外部空間OSに漏れる可能性がある。 If the anaerobic chamber 10 is damaged, hydrogen may leak from the anaerobic chamber 10. As a typical example, the anaerobic chamber 10 is provided with an operation glove, and the tip of the glove becomes thin due to wear or the like, and a hole can be opened there. A relatively large amount of hydrogen may leak from the internal space IS to the external space OS through such holes.
検知部70は、内部空間ISから外部空間OSへの水素の漏れを内部空間ISの圧力の低下による嫌気性チャンバー10の変形に基づいて検知する。より具体的には、検知部70は、例えば、嫌気性チャンバー10の天井部12の高さの変化を計測するように構成されうる。内部空間ISから外部空間OSに水素が漏れると内部空間ISの圧力が低下し、これによって、図2に例示されているように、嫌気性チャンバー10の天井部12の高さが下がりうる。よって、検知部70は、嫌気性チャンバー10の天井部12の高さの変化を計測することによって、内部空間ISから外部空間OSへの水素の漏れを検知することができる。検知部70は、例えば、嫌気性チャンバー10の天井部12の高さが予め設定された閾値を下回ったときに内部空間ISから外部空間OSへの水素の漏れがあったと判断するように構成されうる。 The detection unit 70 detects the leakage of hydrogen from the internal space IS to the external space OS based on the deformation of the anaerobic chamber 10 due to a decrease in the pressure of the internal space IS. More specifically, the detection unit 70 can be configured to measure a change in the height of the ceiling portion 12 of the anaerobic chamber 10, for example. When hydrogen leaks from the internal space IS to the external space OS, the pressure of the internal space IS decreases, and as a result, the height of the ceiling portion 12 of the anaerobic chamber 10 can be decreased as illustrated in FIG. Therefore, the detection unit 70 can detect the leakage of hydrogen from the internal space IS to the external space OS by measuring the change in the height of the ceiling portion 12 of the anaerobic chamber 10. For example, the detection unit 70 is configured to determine that hydrogen has leaked from the internal space IS to the external space OS when the height of the ceiling 12 of the anaerobic chamber 10 falls below a preset threshold value. sell.
検知部70は、例えば、嫌気性チャンバー10の周囲に設けられたフレーム50の上部に取り付けられうる。検知部70は、例えば、超音波を用いて距離を計測する超音波センサを含みうる。 The detection part 70 can be attached to the upper part of the flame | frame 50 provided around the anaerobic chamber 10, for example. The detection unit 70 can include, for example, an ultrasonic sensor that measures a distance using ultrasonic waves.
警報部80は、検知部70による水素の漏れの検知に応じて警報を発する。警報は、例えば、音および/または光によってなされうる。あるいは、警報は、他の装置への警報情報の送信であってもよい。 The alarm unit 80 issues an alarm in response to detection of hydrogen leakage by the detection unit 70. The alarm can be made, for example, by sound and / or light. Alternatively, the alarm may be transmission of alarm information to another device.
100 嫌気培養装置
10 嫌気性チャンバー
12 天井部
20 触媒
30 環境制御部
50 フレーム
60 棚
70 検知部
80 警報部
IS 内部空間
OS 外部空間
DESCRIPTION OF SYMBOLS 100 Anaerobic culture apparatus 10 Anaerobic chamber 12 Ceiling part 20 Catalyst 30 Environment control part 50 Frame 60 Shelf 70 Detection part 80 Alarm part IS Internal space OS External space
Claims (3)
前記内部空間に配置された触媒が水素と酸素との反応を促進させることによって前記内部空間における酸素の濃度が低下するように、前記内部空間に水素を供給する環境制御部と、
前記内部空間から前記外部空間への水素の漏れを前記内部空間の圧力の低下による前記嫌気性チャンバーの変形に基づいて検知する検知部と、
前記検知部による水素の漏れの検知に応じて警報を発する警報部と、
を備えることを特徴とする嫌気培養装置。 An anaerobic chamber configured by a flexible member and partitioning the internal space from the external space;
An environmental control unit for supplying hydrogen to the internal space such that the catalyst disposed in the internal space promotes the reaction between hydrogen and oxygen to reduce the concentration of oxygen in the internal space;
A detection unit for detecting hydrogen leakage from the internal space to the external space based on deformation of the anaerobic chamber due to a decrease in pressure in the internal space;
An alarm unit that issues an alarm in response to detection of hydrogen leakage by the detection unit;
An anaerobic culture apparatus comprising:
ことを特徴とする請求項1に記載の嫌気培養装置。 The detection unit is configured to measure a change in height of the ceiling portion of the anaerobic chamber,
The anaerobic culture apparatus according to claim 1.
ことを特徴とする請求項1又は2に記載の嫌気培養装置。 The detection unit includes an ultrasonic sensor that measures a distance using ultrasonic waves.
The anaerobic culture apparatus according to claim 1 or 2.
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US4111753A (en) * | 1976-06-07 | 1978-09-05 | National Appliance Company | Controlled atmosphere apparatus and method of transferring specimens to same |
JPH03150443A (en) * | 1989-11-08 | 1991-06-26 | Nec Corp | Globe box and analyzing method for gas using the same |
US6100083A (en) * | 1999-03-16 | 2000-08-08 | Coy Laboratory Products, Inc. | Anaerobic chamber with collapsible diaphragm |
CN1965220A (en) * | 2004-06-07 | 2007-05-16 | 检测机械(第二)集团有限公司 | An apparatus and method for testing flexible packages for defects |
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