JP2015015904A - Anaerobic culture apparatus - Google Patents

Anaerobic culture apparatus Download PDF

Info

Publication number
JP2015015904A
JP2015015904A JP2013143081A JP2013143081A JP2015015904A JP 2015015904 A JP2015015904 A JP 2015015904A JP 2013143081 A JP2013143081 A JP 2013143081A JP 2013143081 A JP2013143081 A JP 2013143081A JP 2015015904 A JP2015015904 A JP 2015015904A
Authority
JP
Japan
Prior art keywords
internal space
hydrogen
anaerobic
culture apparatus
anaerobic chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2013143081A
Other languages
Japanese (ja)
Other versions
JP6092726B2 (en
Inventor
嘉伸 伊藤
Yoshinobu Ito
嘉伸 伊藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asahi Breweries Ltd
Original Assignee
Asahi Breweries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Breweries Ltd filed Critical Asahi Breweries Ltd
Priority to JP2013143081A priority Critical patent/JP6092726B2/en
Publication of JP2015015904A publication Critical patent/JP2015015904A/en
Application granted granted Critical
Publication of JP6092726B2 publication Critical patent/JP6092726B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

PROBLEM TO BE SOLVED: To detect hydrogen leakage from an anaerobic chamber further securely.SOLUTION: An anaerobic culture apparatus 100 comprises: an anaerobic chamber 10 which is constituted of a flexible member and partitions an internal space IS from an outside space OS; an environmental control part 30 which supplies hydrogen to the internal space IS such that concentration of oxygen in the internal space IS is reduced by promoting reaction between hydrogen and oxygen by a catalyst 20 placed in the internal space IS; a detection part 70 which detects hydrogen leakage from the internal space IS to the outside space OS on the basis of a deformation of the anaerobic chamber 10 caused by pressure reduction of the internal space IS; and an alarm part 80 which issues an alarm correspondingly to the detection of hydrogen leakage by the detection part 70.

Description

本発明は、嫌気培養装置に関する。   The present invention relates to an anaerobic culture apparatus.

微生物などを嫌気環境で培養するための嫌気培養装置がある。嫌気培養装置の1つとして、内部空間を外部空間から仕切る嫌気性チャンバーと、内部空間に水素を供給するユニットとを備えるものがある。内部空間には、触媒が配置されていて、この触媒が水素と酸素との反応を促進させることによって内部空間における酸素の濃度が低下する。   There is an anaerobic culture apparatus for culturing microorganisms in an anaerobic environment. One anaerobic culture apparatus includes an anaerobic chamber that partitions an internal space from an external space, and a unit that supplies hydrogen to the internal space. A catalyst is disposed in the internal space, and this catalyst promotes the reaction between hydrogen and oxygen, so that the concentration of oxygen in the internal space decreases.

特開2006−217806号JP 2006-217806 A 特開2006−149232号JP 2006-149232 A

上記のような嫌気培養装置において、嫌気性チャンバーが破損し、内部空間から外部空間に水素が漏れ続けると、危険である。そこで、嫌気培養装置が配置された部屋に水素センサを配置して、これによって水素を検知することが考えられる。しかしながら、例えば、当該部屋に空気の流れがあると、嫌気性チャンバーからの水素の漏れを確実に検知することが難しい。   In the anaerobic culture apparatus as described above, it is dangerous if the anaerobic chamber is damaged and hydrogen continues to leak from the internal space to the external space. Therefore, it is conceivable that a hydrogen sensor is arranged in a room where an anaerobic culture apparatus is arranged to detect hydrogen. However, for example, if there is an air flow in the room, it is difficult to reliably detect hydrogen leakage from the anaerobic chamber.

本発明は、嫌気性チャンバーからの水素の漏れをより確実に検知することを目的とする。   An object of the present invention is to more reliably detect hydrogen leakage from an anaerobic chamber.

本発明は、嫌気培養装置に係り、前記嫌気培養装置は、可撓性部材によって構成され内部空間を外部空間から仕切る嫌気性チャンバーと、前記内部空間に配置された触媒が水素と酸素との反応を促進させることによって前記内部空間における酸素の濃度が低下するように、前記内部空間に水素を供給する環境制御部と、前記内部空間から前記外部空間への水素の漏れを前記内部空間の圧力の低下による前記嫌気性チャンバーの変形に基づいて検知する検知部と、前記検知部による水素の漏れの検知に応じて警報を発する警報部と、を備える。   The present invention relates to an anaerobic culture apparatus, wherein the anaerobic culture apparatus is constituted by a flexible member and partitions an internal space from an external space, and a catalyst disposed in the internal space reacts with hydrogen and oxygen. The environmental control unit for supplying hydrogen to the internal space and the leakage of hydrogen from the internal space to the external space to reduce the pressure of the internal space. A detection unit that detects based on deformation of the anaerobic chamber due to a decrease, and an alarm unit that issues an alarm in response to detection of hydrogen leakage by the detection unit.

本発明によれば、嫌気性チャンバーからの水素の漏れをより確実に検知する。   According to the present invention, leakage of hydrogen from the anaerobic chamber is more reliably detected.

本発明の1つの実施形態の嫌気培養装置の構成を模式的に示す図。The figure which shows typically the structure of the anaerobic culture apparatus of one embodiment of this invention. 本発明の1つの実施形態の嫌気培養装置において水素が漏れている状態を模式的に示す図。The figure which shows typically the state in which hydrogen is leaking in the anaerobic culture apparatus of one embodiment of this invention.

以下、添付図面を参照しながら本発明をその例示的な実施形態の嫌気培養装置100を通して説明する。   Hereinafter, the present invention will be described through an anaerobic culture apparatus 100 of an exemplary embodiment with reference to the accompanying drawings.

嫌気培養装置100は、嫌気性チャンバー10と、環境制御部30と、検知部70と、警報部80とを備えている。嫌気性チャンバー10は、可撓性部材によって構成され、内部空間ISを外部空間OSから仕切る。可撓性部材は、例えば、塩化ビニールで形成されうる。内部空間ISには、水素と酸素との反応を促進させるための触媒20が配置される。触媒20は、例えば、内部空間ISに配置された棚60に置かれうる。   The anaerobic culture apparatus 100 includes an anaerobic chamber 10, an environment control unit 30, a detection unit 70, and an alarm unit 80. The anaerobic chamber 10 is configured by a flexible member, and partitions the internal space IS from the external space OS. The flexible member can be formed of, for example, vinyl chloride. A catalyst 20 for accelerating the reaction between hydrogen and oxygen is disposed in the internal space IS. The catalyst 20 can be placed on, for example, a shelf 60 disposed in the internal space IS.

環境制御部30は、内部空間ISに配置された触媒20が水素と酸素との反応を促進させることによって内部空間ISにおける酸素の濃度が低下するように、内部空間ISに水素を供給する。一例において、環境制御部30は、まず、内部空間ISの空気を窒素ガスで置換し、その後に、触媒20の作用により内部空間ISにおける酸素の濃度が低下するように、内部空間ISに水素を供給する。   The environment control unit 30 supplies hydrogen to the internal space IS such that the catalyst 20 disposed in the internal space IS promotes the reaction between hydrogen and oxygen, so that the oxygen concentration in the internal space IS decreases. In one example, the environment control unit 30 first replaces the air in the internal space IS with nitrogen gas, and then supplies hydrogen to the internal space IS so that the concentration of oxygen in the internal space IS decreases due to the action of the catalyst 20. Supply.

嫌気性チャンバー10が損傷を受けると、嫌気性チャンバー10から水素が漏れうる。典型的な例としては、嫌気性チャンバー10には操作用のグローブが設けられていて、そのグローブの先端等が摩耗等によって薄くなって、そこに穴が開きうる。このような穴を通して比較的多くの水素が内部空間ISから外部空間OSに漏れる可能性がある。   If the anaerobic chamber 10 is damaged, hydrogen may leak from the anaerobic chamber 10. As a typical example, the anaerobic chamber 10 is provided with an operation glove, and the tip of the glove becomes thin due to wear or the like, and a hole can be opened there. A relatively large amount of hydrogen may leak from the internal space IS to the external space OS through such holes.

検知部70は、内部空間ISから外部空間OSへの水素の漏れを内部空間ISの圧力の低下による嫌気性チャンバー10の変形に基づいて検知する。より具体的には、検知部70は、例えば、嫌気性チャンバー10の天井部12の高さの変化を計測するように構成されうる。内部空間ISから外部空間OSに水素が漏れると内部空間ISの圧力が低下し、これによって、図2に例示されているように、嫌気性チャンバー10の天井部12の高さが下がりうる。よって、検知部70は、嫌気性チャンバー10の天井部12の高さの変化を計測することによって、内部空間ISから外部空間OSへの水素の漏れを検知することができる。検知部70は、例えば、嫌気性チャンバー10の天井部12の高さが予め設定された閾値を下回ったときに内部空間ISから外部空間OSへの水素の漏れがあったと判断するように構成されうる。   The detection unit 70 detects the leakage of hydrogen from the internal space IS to the external space OS based on the deformation of the anaerobic chamber 10 due to a decrease in the pressure of the internal space IS. More specifically, the detection unit 70 can be configured to measure a change in the height of the ceiling portion 12 of the anaerobic chamber 10, for example. When hydrogen leaks from the internal space IS to the external space OS, the pressure of the internal space IS decreases, and as a result, the height of the ceiling portion 12 of the anaerobic chamber 10 can be decreased as illustrated in FIG. Therefore, the detection unit 70 can detect the leakage of hydrogen from the internal space IS to the external space OS by measuring the change in the height of the ceiling portion 12 of the anaerobic chamber 10. For example, the detection unit 70 is configured to determine that hydrogen has leaked from the internal space IS to the external space OS when the height of the ceiling 12 of the anaerobic chamber 10 falls below a preset threshold value. sell.

検知部70は、例えば、嫌気性チャンバー10の周囲に設けられたフレーム50の上部に取り付けられうる。検知部70は、例えば、超音波を用いて距離を計測する超音波センサを含みうる。   The detection part 70 can be attached to the upper part of the flame | frame 50 provided around the anaerobic chamber 10, for example. The detection unit 70 can include, for example, an ultrasonic sensor that measures a distance using ultrasonic waves.

警報部80は、検知部70による水素の漏れの検知に応じて警報を発する。警報は、例えば、音および/または光によってなされうる。あるいは、警報は、他の装置への警報情報の送信であってもよい。   The alarm unit 80 issues an alarm in response to detection of hydrogen leakage by the detection unit 70. The alarm can be made, for example, by sound and / or light. Alternatively, the alarm may be transmission of alarm information to another device.

100 嫌気培養装置
10 嫌気性チャンバー
12 天井部
20 触媒
30 環境制御部
50 フレーム
60 棚
70 検知部
80 警報部
IS 内部空間
OS 外部空間
DESCRIPTION OF SYMBOLS 100 Anaerobic culture apparatus 10 Anaerobic chamber 12 Ceiling part 20 Catalyst 30 Environment control part 50 Frame 60 Shelf 70 Detection part 80 Alarm part IS Internal space OS External space

Claims (3)

可撓性部材によって構成され内部空間を外部空間から仕切る嫌気性チャンバーと、
前記内部空間に配置された触媒が水素と酸素との反応を促進させることによって前記内部空間における酸素の濃度が低下するように、前記内部空間に水素を供給する環境制御部と、
前記内部空間から前記外部空間への水素の漏れを前記内部空間の圧力の低下による前記嫌気性チャンバーの変形に基づいて検知する検知部と、
前記検知部による水素の漏れの検知に応じて警報を発する警報部と、
を備えることを特徴とする嫌気培養装置。
An anaerobic chamber configured by a flexible member and partitioning the internal space from the external space;
An environmental control unit for supplying hydrogen to the internal space such that the catalyst disposed in the internal space promotes the reaction between hydrogen and oxygen to reduce the concentration of oxygen in the internal space;
A detection unit for detecting hydrogen leakage from the internal space to the external space based on deformation of the anaerobic chamber due to a decrease in pressure in the internal space;
An alarm unit that issues an alarm in response to detection of hydrogen leakage by the detection unit;
An anaerobic culture apparatus comprising:
前記検知部は、前記嫌気性チャンバーの天井部の高さの変化を計測するように構成されている、
ことを特徴とする請求項1に記載の嫌気培養装置。
The detection unit is configured to measure a change in height of the ceiling portion of the anaerobic chamber,
The anaerobic culture apparatus according to claim 1.
前記検知部は、超音波を用いて距離を計測する超音波センサを含む、
ことを特徴とする請求項1又は2に記載の嫌気培養装置。
The detection unit includes an ultrasonic sensor that measures a distance using ultrasonic waves.
The anaerobic culture apparatus according to claim 1 or 2.
JP2013143081A 2013-07-08 2013-07-08 Anaerobic culture device Active JP6092726B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2013143081A JP6092726B2 (en) 2013-07-08 2013-07-08 Anaerobic culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2013143081A JP6092726B2 (en) 2013-07-08 2013-07-08 Anaerobic culture device

Publications (2)

Publication Number Publication Date
JP2015015904A true JP2015015904A (en) 2015-01-29
JP6092726B2 JP6092726B2 (en) 2017-03-08

Family

ID=52437590

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013143081A Active JP6092726B2 (en) 2013-07-08 2013-07-08 Anaerobic culture device

Country Status (1)

Country Link
JP (1) JP6092726B2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4111753A (en) * 1976-06-07 1978-09-05 National Appliance Company Controlled atmosphere apparatus and method of transferring specimens to same
JPH03150443A (en) * 1989-11-08 1991-06-26 Nec Corp Globe box and analyzing method for gas using the same
US6100083A (en) * 1999-03-16 2000-08-08 Coy Laboratory Products, Inc. Anaerobic chamber with collapsible diaphragm
US20080127716A1 (en) * 2004-06-07 2008-06-05 Matts Eliasson Apparatus and Method for Testing Flexible Packages for Defects
JP2012073197A (en) * 2010-09-30 2012-04-12 Hitachi High-Technologies Corp Bacteriological examination system

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4111753A (en) * 1976-06-07 1978-09-05 National Appliance Company Controlled atmosphere apparatus and method of transferring specimens to same
JPH03150443A (en) * 1989-11-08 1991-06-26 Nec Corp Globe box and analyzing method for gas using the same
US6100083A (en) * 1999-03-16 2000-08-08 Coy Laboratory Products, Inc. Anaerobic chamber with collapsible diaphragm
US20080127716A1 (en) * 2004-06-07 2008-06-05 Matts Eliasson Apparatus and Method for Testing Flexible Packages for Defects
JP2012073197A (en) * 2010-09-30 2012-04-12 Hitachi High-Technologies Corp Bacteriological examination system

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
金内長司: "嫌気性グローブボックス法", 感染症学雑誌, vol. 47, JPN6017002625, 1973, pages 125 - 130, ISSN: 0003488455 *

Also Published As

Publication number Publication date
JP6092726B2 (en) 2017-03-08

Similar Documents

Publication Publication Date Title
TW200628775A (en) Method and system for detecting leak in electronic devices
RU2015106861A (en) DEVICE (OPTIONS) FOR DETECTING THE LEAK OF A REGULATING DEVICE FOR A FLUID
MX2012005122A (en) Refrigerant leak detection system and method.
EP2677314A3 (en) Chemical sensor in a portable electronic device
MX2012004666A (en) Downhole monitoring with distributed acoustic/vibration, strain and/or density sensing.
MX2019011676A (en) Method and apparatus for acoustically detecting fluid leaks.
MX340267B (en) Pressure based gas leak testing.
EP2423547A3 (en) Cutoff valve control apparatus
MX336147B (en) Sealing systems for turboexpanders for use in organic rankine cycles.
MY167512A (en) Chamber condition
EP2157298A3 (en) A method for detecting overpressure inside a compartment associated with a gas turbine nacelle
CA2854318C (en) Method and system for detecting a leak of fluid from a fluid-carrying duct
GB201115075D0 (en) Closed circuit breathing apparatus and method of operating the same
JP6092726B2 (en) Anaerobic culture device
WO2012027406A3 (en) Vacuum chamber assembly for supporting a workpiece
TR201909377T4 (en) Top blown lance use injector reactor system with a sensing device for determining an operating condition in a melt bath.
WO2008120399A1 (en) Hermetic closure structure
JP2012127882A (en) Leakage detection device and leakage detection method
KR20140124523A (en) Fuel supplying apparatus
GB2489176A (en) Leak detection device
TW200718934A (en) Apparatus for sensing plural gases
CN103364156B (en) Multi-usage air-tightness detection device
GB2509042A (en) Device and system for gas leakage detection and alarm
MY167836A (en) Instrumentation system for determining risk factors
KR20160001590U (en) Apparatus for detecting leakage of double wall pipe by using internal air flow

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160524

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20170119

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20170203

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20170209

R150 Certificate of patent or registration of utility model

Ref document number: 6092726

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250