JP6026822B2 - Absorbent structure and absorbent material - Google Patents
Absorbent structure and absorbent material Download PDFInfo
- Publication number
- JP6026822B2 JP6026822B2 JP2012188131A JP2012188131A JP6026822B2 JP 6026822 B2 JP6026822 B2 JP 6026822B2 JP 2012188131 A JP2012188131 A JP 2012188131A JP 2012188131 A JP2012188131 A JP 2012188131A JP 6026822 B2 JP6026822 B2 JP 6026822B2
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- Prior art keywords
- detection reagent
- absorbent
- activity detection
- urine
- enzyme
- Prior art date
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- 239000002250 absorbent Substances 0.000 title claims description 126
- 230000002745 absorbent Effects 0.000 title claims description 126
- 239000000463 material Substances 0.000 title description 33
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- 230000000694 effects Effects 0.000 claims description 80
- 102000004190 Enzymes Human genes 0.000 claims description 76
- 108090000790 Enzymes Proteins 0.000 claims description 76
- 239000003153 chemical reaction reagent Substances 0.000 claims description 72
- 238000001514 detection method Methods 0.000 claims description 72
- 150000001875 compounds Chemical class 0.000 claims description 59
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 239000000975 dye Substances 0.000 claims description 22
- 238000006911 enzymatic reaction Methods 0.000 claims description 20
- 238000010521 absorption reaction Methods 0.000 claims description 19
- 108060007951 sulfatase Proteins 0.000 claims description 19
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- 108010031186 Glycoside Hydrolases Proteins 0.000 claims description 11
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- 239000002243 precursor Substances 0.000 claims description 8
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- BXFFHSIDQOFMLE-UHFFFAOYSA-N indoxyl sulfate Chemical compound C1=CC=C2C(OS(=O)(=O)O)=CNC2=C1 BXFFHSIDQOFMLE-UHFFFAOYSA-N 0.000 claims description 6
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- GAZVSOQUFLIBJQ-BYNIDDHOSA-N (2s,3s,4s,5r,6s)-6-[(5-bromo-6-chloro-1h-indol-3-yl)oxy]-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound O1[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1OC1=CNC2=CC(Cl)=C(Br)C=C12 GAZVSOQUFLIBJQ-BYNIDDHOSA-N 0.000 claims description 2
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Landscapes
- Absorbent Articles And Supports Therefor (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
本発明は、尿臭発生部を簡便かつ視覚的に特定できる吸収性構造体に関する。 The present invention relates to an absorbent structure capable of easily and visually specifying a urine odor generating part.
近年、消費者の衛生志向の高まりから、見た目の汚ればかりでなく汚れの存在を想起させる臭気についても、これを除去することが強く望まれている。特に尿については、排尿直後の臭気は弱いが、これが例えば使い捨ておむつ、尿取りパッド等の吸収性物品中に吸収された状態では、時間の経過に伴い強い臭気を発するようになることが知られており、この不快度は生活環境悪臭の中でもとりわけ大きい。
このような時間の経過に伴い発生する尿臭気は、臭気成分前駆体となる尿中の種々の有機物が微生物の酵素によって分解されることに起因するものと考えられている。このため、これに対する消臭・防臭技術としては、悪臭成分を物理的又は化学的に除去する消臭剤や、悪臭を感覚的にマスキングする香料の使用だけでなく、悪臭の発生を元から持続的に抑制できる手法として、臭気原因微生物に対する抗菌剤や原因酵素（ウレアーゼ、β-グルクロニダーゼ、アリールサルファターゼ等）に対する酵素阻害剤の使用も提案されている。
In recent years, it has been strongly desired to remove not only the appearance of dirt but also the odor reminiscent of the presence of dirt due to the increase in consumer hygiene. Especially for urine, although the odor immediately after urination is weak, it is known that when it is absorbed in an absorbent article such as a disposable diaper or a urine collection pad, a strong odor will be emitted over time. This discomfort level is particularly great among the odors in the living environment.
The urine odor generated with the passage of time is considered to be caused by the decomposition of various organic substances in urine, which are odor component precursors, by microbial enzymes. For this reason, as a deodorizing and deodorizing technology for this, not only the use of deodorizers that physically or chemically remove malodorous components or fragrances that mask malodorous sensations, but also the generation of malodors from the beginning. As a technique that can be suppressed, the use of antibacterial agents against odor-causing microorganisms and enzyme inhibitors against causative enzymes (urease, β-glucuronidase, arylsulfatase, etc.) has been proposed.
一方、微生物に由来する臭気やこれに関わる微生物を簡便に検出する手法としては、例えば特許文献１では、酸塩基指示薬等の臭気感色性の視覚指示薬（臭気成分と反応することにより色変化を生じる化合物）を含む使い捨ておむつ、失禁パッド等の製品が開示されている。 On the other hand, as a technique for easily detecting odors derived from microorganisms and microorganisms related thereto, for example, in Patent Document 1, an odor-sensitive visual indicator (such as an acid-base indicator) (color change is caused by reacting with an odor component). Products such as disposable diapers and incontinence pads containing the resulting compound) are disclosed.
また、特許文献２では細菌に由来する揮発性成分をＧＣ分析あるいは指示薬で検出することによって微生物の存在を検出する手法が開示されている。この手法によれば、例えば大腸菌の存在は、ジメチルアミノシンナミックアルデヒドで染色された綿を大腸菌の懸濁液に露出させ、菌体の産するインドールによる指示薬の変色によって確認される。 Patent Document 2 discloses a technique for detecting the presence of microorganisms by detecting a volatile component derived from bacteria by GC analysis or an indicator. According to this technique, for example, the presence of E. coli is confirmed by exposing cotton stained with dimethylaminocinnamic aldehyde to a suspension of E. coli and discoloring the indicator by the indole produced by the cells.
また、特許文献３ではβ-グルクロニダーゼ又はアリールサルファターゼの基質型酵素活性検出試薬を含有する培地によって、大腸菌を含む尿路感染症関連細菌を検出する方法が提案されている。この手法では前述の培地に検体を加えて培養し、菌体酵素に由来するコロニー及び／又は培地の着色を目視観察することによって微生物の検出を行う。 Patent Document 3 proposes a method for detecting urinary tract infection-related bacteria including Escherichia coli using a medium containing a reagent for detecting substrate-type enzyme activity of β-glucuronidase or arylsulfatase. In this method, the specimen is added to the above-mentioned medium and cultured, and microorganisms are detected by visually observing the colony derived from the bacterial cell enzyme and / or the color of the medium.
前述のように、尿臭気の消臭・防臭技術としては、消臭剤、マスキング剤、抗菌剤、酵素阻害剤等、様々な剤の使用が考えられるが、これら剤の適用部位を決定する際、尿臭発生部の位置情報は非常に重要である。とりわけ抗菌剤、酵素阻害剤のような防臭剤は、剤の適用部位が臭気発生部から外れると著しく効果が落ちる場合があり、臭気発生部への適用は必須と言える。また、特に吸収性物品のように尿が様々な素材と接触しながら移動していく製品について、消臭・防臭剤の配合部位を決定するためには、尿臭発生部を厳密に特定する必要がある。 As mentioned above, various deodorant, masking agent, antibacterial agent, enzyme inhibitor, etc. can be used as deodorizing / deodorizing technology for urine odor. The position information of the urine odor generating part is very important. In particular, deodorizers such as antibacterial agents and enzyme inhibitors may be significantly less effective when the application site of the agent is removed from the odor generating part, and it can be said that application to the odor generating part is essential. In addition, it is necessary to strictly identify the urine odor generating part in order to determine the deodorant / deodorant blending site for products that move while contacting urine with various materials, such as absorbent articles. There is.
このように、吸収性物品における簡便な尿臭発生部の可視化手法は、消臭・防臭剤の配合部位や配合量の最適化のため強く望まれる技術であるが、一方で、臭気成分には強い拡散性があるため、例えば特許文献１及び２のように臭気成分自体を指示薬あるいはＧＣ分析等によって検出する手法では、高い空間分解能で臭気発生部を特定することは非常に困難である。 As described above, the simple visualization technique of the urine odor generating part in the absorbent article is a technique that is strongly desired for optimizing the blending part and blending amount of the deodorant / deodorant. Due to the strong diffusivity, it is very difficult to identify the odor generating part with high spatial resolution by the technique of detecting the odor component itself by an indicator or GC analysis as in Patent Documents 1 and 2, for example.
また、特許文献３のように培地での培養を前提とする方法は、微生物の増殖部位として臭気発生部の特定は可能であるが、求める空間分解能に応じてサンプルを細かく分割等する必要があることから操作は煩雑となり、吸収性物品の製品と同様の系における尿臭発生部の特定は困難である。 Further, the method based on the culture in the medium as in Patent Document 3 can specify the odor generating part as the growth site of the microorganism, but it is necessary to finely divide the sample according to the required spatial resolution. Therefore, the operation becomes complicated, and it is difficult to specify the urine odor generating part in the same system as the product of the absorbent article.
従って、本発明の課題は、尿臭発生部を簡便且つ視覚的に特定することのできる吸収性構造体を提供することにある。 Therefore, the subject of this invention is providing the absorptive structure which can specify a urine odor generating part simply and visually.
本発明は、臭気成分前駆体を分解して臭気成分を生じる酵素による酵素反応によって水に難溶の色素化合物又は蛍光色素化合物を生じる酵素基質型の酵素活性検出試薬を含有する吸収性構造体を提供するものである。
また、本発明は、臭気成分前駆体を分解して臭気成分を生じる酵素による酵素反応によって水に難溶の色素化合物又は蛍光色素化合物を生じる酵素基質型の酵素活性検出試薬を保持する吸収材を提供するものである。
The present invention provides an absorptive structure containing an enzyme substrate type enzyme activity detection reagent that generates a dye compound or a fluorescent dye compound that is hardly soluble in water by an enzyme reaction by an enzyme that decomposes an odor component precursor to generate an odor component. It is to provide.
Further, the present invention provides an absorbent material for holding an enzyme substrate type enzyme activity detection reagent that generates a dye compound or a fluorescent dye compound that is hardly soluble in water by an enzyme reaction that generates an odor component by decomposing the odor component precursor. It is to provide.
本発明の吸収性構造体によれば、時間の経過に伴い発生する尿臭の発生部位を、簡便かつ客観的に特定することができる。そのため、例えば、種々の消臭剤、芳香剤、抗菌剤、酵素阻害剤の好ましい配合部位やその配合量等の決定が容易となり、また、尿臭生成抑制効果に優れた製品構成や吸収材の選択等が容易となる。
また、本発明の吸収材によれば、尿臭発生部を特定したい対象に分散させることによって、該尿臭発生部を簡便かつ客観的に特定することができる。
According to the absorptive structure of the present invention, it is possible to easily and objectively specify a site where urine odor is generated over time. Therefore, for example, it is easy to determine the preferred blending sites and blending amounts of various deodorants, fragrances, antibacterial agents, enzyme inhibitors, etc. Selection becomes easy.
Moreover, according to the absorbent material of the present invention, the urine odor generating part can be easily and objectively specified by dispersing the urine odor generating part in the target to be specified.
以下、本発明をその好ましい実施形態に基づいて説明する。 Hereinafter, the present invention will be described based on preferred embodiments thereof.
＜酵素活性検出試薬を含ませる対象の吸収性構造体及び吸収材＞
本発明の吸収性構造体は、好ましくは吸収性物品又は該吸収性物品の吸収性コアである。
吸収性物品は、使用時にヒト又は動物由来の体液が吸収される物品であり、好ましくは尿及び汗の一方又は双方を含有する体液、より好ましくは尿を含有する体液、特に尿が吸収される物品である。
吸収性物品としては、軽失禁パッド、尿取りパッド、使い捨ておむつ、パンティーライナー、生理用ナプキン、タンポン等のサニタリー製品、ペット用トイレ、ペット用尿吸収シート等の犬、猫等のペット用排泄物関連製品を挙げることができる。これらは、尿あるいは尿を含む体液が吸収保持されることが想定されるものであり、本発明の吸収性物品として好ましい。同様の観点から、軽失禁パッド、尿取りパッド、使い捨ておむつ、ペット用トイレ、ペット用尿吸収シートがより好ましい。
<Absorptive structure and absorbent material for inclusion of enzyme activity detection reagent>
The absorbent structure of the present invention is preferably an absorbent article or an absorbent core of the absorbent article.
Absorbent articles are articles in which body fluids derived from humans or animals are absorbed during use, preferably body fluids containing one or both of urine and sweat, more preferably body fluids containing urine, especially urine. It is an article.
Absorbent articles include light incontinence pads, urine-absorbing pads, disposable diapers, panty liners, sanitary products such as sanitary napkins, tampons, pet toilets, pet excrement such as dogs and cats such as pet urine absorbent sheets List relevant products. These are supposed to absorb and hold urine or body fluid containing urine, and are preferable as the absorbent article of the present invention. From the same viewpoint, a light incontinence pad, a urine collecting pad, a disposable diaper, a pet toilet, and a pet urine absorbing sheet are more preferable.
図１は、本発明の吸収性構造体の一実施形態である吸収性物品の断面構造の一例を示す模式断面図である。図１に示す吸収性物品１は、使い捨ておむつ、軽失禁パッド又は尿取りパッド等であり、図１に示すように、液透過性の表面シート２、液難透過性の裏面シート３、及びこれら両シート２，３間に介在された吸収性コア４を具備している。液難透過性は、液不透過性と液難透過性を含む概念である。また、図１に示す吸収性コア４は、パルプ繊維等の繊維材料４２及び吸水性ポリマー４３からなり、その上下両面をコアラップシート４４，４５によって被覆されている。吸収性コア４は、例えば、積繊ドラムに向けて飛散状態で供給した繊維材料４２及び吸水性ポリマー４３を、該積繊ドラムの周面に形成した凹部に吸引により堆積させることにより、所定形状に成形した混合積繊体である。コアラップシート４４，４５は、主として吸水性ポリマー４３の漏れ出しを防止するものであり、好ましくは、吸収性物品の幅方向に沿う断面において、吸収性コア４の周囲を全周に亘って被覆している。コアラップシート４４，４５は、例えば、薄葉紙又は液透過性の不織布等からなり、吸収性コア４の表面シート２側を被覆する部分と該コアの裏面シート３側を被覆する部分とが、一枚のシートからなるものでも別体のシートからなるものでも良い。また、本発明における吸収性物品の吸収性コアとしては、各種公知の吸収性物品の吸収性コアの構成を採用でき、例えば、図２に示す吸収性コア４１のように、吸水性ポリマー４３が厚み方向の一部に偏在していても良いし、繊維材料の密度又は種類が異なる複数の繊維層４６，４７を有するもの等であっても良い。 FIG. 1 is a schematic cross-sectional view showing an example of a cross-sectional structure of an absorbent article which is an embodiment of the absorbent structure of the present invention. The absorbent article 1 shown in FIG. 1 is a disposable diaper, a light incontinence pad, a urine picking pad, or the like. As shown in FIG. 1, a liquid-permeable top sheet 2, a liquid-permeable back sheet 3, and these An absorbent core 4 interposed between the two sheets 2 and 3 is provided. The liquid impermeability is a concept including liquid impermeability and liquid impermeability. Moreover, the absorptive core 4 shown in FIG. 1 consists of fiber materials 42, such as a pulp fiber, and the water absorbing polymer 43, and the upper and lower surfaces are coat | covered with the core wrap sheets 44 and 45. As shown in FIG. The absorbent core 4 has a predetermined shape by, for example, depositing the fiber material 42 and the water-absorbing polymer 43 supplied in a scattered state toward the stacking drum by suction in a recess formed on the peripheral surface of the stacking drum. It is a mixed product formed into a fiber. The core wrap sheets 44 and 45 mainly prevent leakage of the water-absorbing polymer 43, and preferably cover the entire periphery of the absorbent core 4 in a cross section along the width direction of the absorbent article. doing. The core wrap sheets 44 and 45 are made of, for example, thin paper or a liquid-permeable nonwoven fabric, and the portion covering the topsheet 2 side of the absorbent core 4 and the portion covering the backsheet 3 side of the core are one. It may be a single sheet or a separate sheet. Moreover, as an absorptive core of the absorptive article in this invention, the structure of the absorptive core of various well-known absorptive articles can be employ | adopted, for example, like the absorptive core 41 shown in FIG. It may be unevenly distributed in a part of the thickness direction, or may have a plurality of fiber layers 46 and 47 having different density or types of fiber materials.
本発明における吸収性構造体には、後述する酵素活性検出試薬が配合されている。例えば、図１に示す構成の吸収性物品にも、後述する酵素活性検出試薬が配合されている。吸収性構造体が、吸収性物品である場合、酵素活性検出試薬は、その吸収性物品のどこに配置されていても良いが、少なくとも吸収性コアに配置されていることが好ましい。
また、酵素活性検出試薬は、尿臭発生部を良好な空間分解能で特定可能とする点から、吸収性物品全体に満遍なく均一に配置されていることが好ましい。また、同様の観点から、酵素活性検出試薬は、吸収性コアの全体に分布していることが好ましく、吸収性コアの全体に均一に配置されていることがより好ましい。吸収性物品全体、又は吸収性コアの全体に均一に配置する方法としては、例えば、後述するように酵素活性検出試薬を溶解した液剤を、吸収性物品全体又は吸収性コアの全体に含浸させた後、乾燥させる方法、あるいは吸収性物品、又は吸収性コアを構成する材料それぞれについて、あらかじめ酵素活性検出試薬を均一に固定化した材料を用いて混合積繊体を構成する方法が挙げられる。あらかじめ吸収性物品又は吸収性コアを構成する材料に酵素活性検出試薬を均一に固定化する方法としては、一般的に行われている方法を用いればよい。例えば酵素活性検出試薬を溶解した液剤を添加し、続いて攪拌を行いながら溶媒を留去することによって酵素活性検出試薬を添着させる方法で固定化することができる。吸収性コアを構成する材料としては、パルプ繊維（フラッフパルプ）等の繊維材料、吸水性ポリマー等が挙げられる。吸収性コアは、繊維材料のみ、又は吸水性ポリマーのみから構成されていても良い。
The absorptive structure in the present invention is mixed with an enzyme activity detection reagent described later. For example, the absorptive article having the configuration shown in FIG. When the absorbent structure is an absorbent article, the enzyme activity detection reagent may be disposed anywhere in the absorbent article, but is preferably disposed at least in the absorbent core.
Moreover, it is preferable that the enzyme activity detection reagent is uniformly and uniformly arranged in the entire absorbent article from the viewpoint that the urine odor generating part can be specified with good spatial resolution. From the same viewpoint, the enzyme activity detection reagent is preferably distributed throughout the absorbent core, and more preferably uniformly disposed throughout the absorbent core. As a method for uniformly disposing the absorbent article as a whole or the absorbent core as a whole, for example, as described later, the liquid absorbent in which the enzyme activity detection reagent is dissolved is impregnated in the absorbent article or absorbent core. Thereafter, a method of drying, or a method of forming a mixed fiber using a material in which an enzyme activity detection reagent is uniformly fixed in advance is used for each of the materials constituting the absorbent article or the absorbent core. As a method for uniformly immobilizing the enzyme activity detection reagent on the material constituting the absorbent article or the absorbent core in advance, a generally performed method may be used. For example, the enzyme activity detection reagent can be immobilized by adding a solution in which the enzyme activity detection reagent is dissolved, and then distilling off the solvent while stirring. Examples of the material constituting the absorbent core include fiber materials such as pulp fibers (fluff pulp), water-absorbing polymers, and the like. The absorptive core may be comprised only from the fiber material or only the water absorbing polymer.
本発明において、吸収材とは、自重の５重量倍以上１０００重量倍以下の体液、特に尿を吸収することができる素材であり、好ましくは、上記したような本発明の吸収性物品の吸収材料として使用される素材である。このような吸収材としては、例えば、吸水性ポリマー、パルプ繊維（フラッフパルプ）、スポンジ等を挙げることができるが、上述の特性を有し、直接的あるいは副次的に尿等の体液を吸収保持する役割を担う素材であれば特に制限されない。
なお、吸水性ポリマーは、自重の２０重量倍以上１０００倍以下の体液、特に尿を吸収して保持できるものである。このような吸水性ポリマーの例としては、各種公知のものを用いることができるが、例えば、アクリル酸又はアクリル酸アルカリ金属塩の重合体又は共重合体の架橋物、ポリアクリル酸及びその塩並びにポリアクリル酸塩グラフト重合体の架橋物、デンプン又はカルボキシメチル化セルロースの架橋物、デンプン-アクリル酸塩グラフト共重合体の加水分解生成物の架橋物、ビニルアルコール-アクリル酸塩共重合体の架橋物、無水マレイン酸グラフトポリビニルアルコールの架橋物、架橋イソブチレン-無水マレイン酸共重合体、酢酸ビニル-アクリル酸エステル共重合体のケン化物から選択される１以上であることが好ましい。
In the present invention, the absorbent material is a material that can absorb body fluid, particularly urine, 5 to 1000 times the weight of its own weight, preferably the absorbent material of the absorbent article of the present invention as described above It is a material used as. Examples of such absorbents include water-absorbing polymers, pulp fibers (fluff pulp), sponges, etc., which have the above-mentioned characteristics and directly or secondaryly absorb body fluids such as urine. The material is not particularly limited as long as the material plays the role of holding.
The water-absorbing polymer is capable of absorbing and holding a body fluid that is 20 to 1000 times its own weight, particularly urine. As examples of such a water-absorbing polymer, various known polymers can be used. For example, a polymer or copolymer cross-linked product of acrylic acid or an alkali metal acrylate, polyacrylic acid and a salt thereof, and the like. Cross-linked product of polyacrylate graft polymer, cross-linked product of starch or carboxymethylated cellulose, cross-linked product of hydrolysis product of starch-acrylate graft copolymer, cross-linked product of vinyl alcohol-acrylate copolymer 1 or more selected from a saponified product of a crosslinked product of maleic anhydride-grafted polyvinyl alcohol, a crosslinked isobutylene-maleic anhydride copolymer, and a vinyl acetate-acrylic ester copolymer.
＜酵素活性検出試薬＞
本発明の吸収性構造体及び吸収材は、尿臭発生部を視覚的に特定可能とする目的で、酵素活性検出試薬を含有する。吸収材は、表面のみに酵素活性検出試薬を保持するものであっても良く、好ましくは、尿臭発生部を特定したい対象に分散させることによって、該対象における尿臭発生部を視覚的に特定可能とする。
<Enzyme activity detection reagent>
The absorbent structure and absorbent material of the present invention contain an enzyme activity detection reagent for the purpose of visually identifying the urine odor generating part. The absorbent material may hold the enzyme activity detection reagent only on the surface. Preferably, the urine odor generating part in the target is visually identified by dispersing the urine odor generating part in the target to be specified. Make it possible.
本発明において酵素活性検出試薬は、尿臭発生部を良好な空間分解能で特定する観点から、拡散の激しい揮発性成分の検出指示薬ではなく、酵素活性を直接的に検出できる酵素基質型の酵素活性検出試薬が用いられる。 In the present invention, the enzyme activity detection reagent is not a detection indicator for a volatile component with high diffusion, but an enzyme substrate type enzyme activity that can directly detect enzyme activity, from the viewpoint of identifying the urine odor generating part with good spatial resolution. A detection reagent is used.
更に本発明において使用する酵素活性検出試薬は、酵素反応により色素化合物又は蛍光色素化合物を生成するものであるが、この色素化合物又は蛍光色素化合物は、上記と同様の観点から、多量の水分を含んだ物品中で拡散性が低いこと、すなわち水に難溶であることを要する。具体的には、上記色素化合物又は蛍光色素化合物は２０℃の水に対する溶解度が１０ｇ／Ｌ未満が好ましく、更には６ｇ／Ｌ未満が好ましく、更には０．５ｇ／Ｌ未満が好ましく、特に０．０５ｇ／Ｌ未満であることが好ましい。 Furthermore, the enzyme activity detection reagent used in the present invention generates a dye compound or a fluorescent dye compound by an enzymatic reaction. This dye compound or fluorescent dye compound contains a large amount of water from the same viewpoint as described above. However, it requires low diffusibility in the article, that is, poorly soluble in water. Specifically, the dye compound or fluorescent dye compound has a solubility in water at 20 ° C. of preferably less than 10 g / L, more preferably less than 6 g / L, even more preferably less than 0.5 g / L, and particularly preferably 0. It is preferably less than 05 g / L.
また、本発明において使用する酵素活性検出試薬は、多量の水分を含んだ物品中で均一に分布していることが好ましい。従って、酵素活性検出試薬は、酵素反応前の時点では水中での拡散性が高いこと、即ち、酵素反応後に生じる色素化合物又は蛍光色素化合物よりも水に対する溶解度が高いことが好ましい。 Moreover, it is preferable that the enzyme activity detection reagent used in the present invention is uniformly distributed in an article containing a large amount of moisture. Therefore, it is preferable that the enzyme activity detection reagent has a high diffusibility in water before the enzyme reaction, that is, has higher solubility in water than a dye compound or a fluorescent dye compound produced after the enzyme reaction.
また、酵素反応により生成される上記化合物は、可視領域に吸収を持つ化合物又は可視領域に蛍光を発する化合物、すなわち色素化合物又は蛍光色素化合物であることを要する。なかでも、検出の容易性から、可視領域に吸収を持つ色素化合物であるものがより好ましい。更に尿の色とのコントラストが明確になることから、特に５００〜８００ｎｍに吸収スペクトルを有する色素化合物が好ましく、青色に呈色するものがより好ましい。 Further, the compound produced by the enzyme reaction needs to be a compound having absorption in the visible region or a compound emitting fluorescence in the visible region, that is, a dye compound or a fluorescent dye compound. Among these, a dye compound having absorption in the visible region is more preferable from the viewpoint of easy detection. Further, since the contrast with the color of urine becomes clear, a dye compound having an absorption spectrum in the range of 500 to 800 nm is particularly preferable, and a blue color is more preferable.
検出の対象となる酵素は、臭気成分前駆体を分解して臭気成分を生じさせる反応を触媒する酵素であり、尿臭発生の原因となる微生物によって産生される酵素であればよく、例えばグリコシダーゼ及びサルファターゼが挙げられる。また、実際に感じる尿臭気の強度、質、尿臭気発生までの時間等を近似的に再現できる観点から、臭気成分としてフェノール化合物の発生部を特定できるものが好ましく、このためβ-グルクロニダーゼあるいはアリールサルファターゼ活性を検出できるものが好ましく、更にはβ-グルクロニダーゼ活性を検出できるものが特に好ましい。
β-グルクロニダーゼは、臭気成分前駆体として尿中に含まれる各種フェノール類のグルクロン酸抱合体を加水分解し、フェノール、p-クレゾール、4-ビニル-2-メトキシフェノール、4-ビニルフェノール、2-メトキシ-1,3-ベンゼンジオール、1,4-ベンゼンジオール、1,3-ベンゼンジオール等のフェノール系臭気成分を生じさせる。また、アリールサルファターゼは臭気成分前駆体として尿中に含まれる各種フェノール類の硫酸抱合体を加水分解し、フェノール、p-クレゾール、4-ビニル-2-メトキシフェノール、4-ビニルフェノール、2-メトキシ-1,3-ベンゼンジオール、1,3-ベンゼンジオール等のフェノール系臭気成分を生じさせる。
The enzyme to be detected is an enzyme that catalyzes a reaction that decomposes the odor component precursor to produce an odor component, and may be an enzyme produced by a microorganism that causes urine odor generation, such as glycosidase and Examples include sulfatase. Further, from the viewpoint of approximating the intensity and quality of urine odor actually felt, the time until urine odor generation, etc., it is preferable to be able to identify the generation part of a phenol compound as an odor component, and therefore β-glucuronidase or aryl Those capable of detecting sulfatase activity are preferred, and those capable of detecting β-glucuronidase activity are particularly preferred.
β-glucuronidase hydrolyzes glucuronic acid conjugates of various phenols contained in urine as an odor component precursor, to produce phenol, p-cresol, 4-vinyl-2-methoxyphenol, 4-vinylphenol, 2-vinylphenol, This produces phenolic odor components such as methoxy-1,3-benzenediol, 1,4-benzenediol and 1,3-benzenediol. Aryl sulfatase hydrolyzes sulfate conjugates of various phenols contained in urine as odor component precursors to produce phenol, p-cresol, 4-vinyl-2-methoxyphenol, 4-vinylphenol, 2-vinylphenol, This produces phenolic odor components such as methoxy-1,3-benzenediol and 1,3-benzenediol.
＜グリコシダーゼ活性検出試薬＞
本発明に用いられるグリコシダーゼ活性検出試薬は、酵素反応後に水に難溶の色素化合物又は蛍光色素化合物を生じる酵素基質型のグリコシダーゼ活性検出試薬であって、グリコシダーゼ活性を視覚的に検出できるものであれば特に限定されない。
<Glycosidase activity detection reagent>
The glycosidase activity detection reagent used in the present invention is an enzyme substrate-type glycosidase activity detection reagent that generates a dye compound or a fluorescent dye compound that is hardly soluble in water after the enzyme reaction, and can detect the glycosidase activity visually. If it does not specifically limit.
酵素反応後に水に難溶の色素化合物又は蛍光色素化合物を生じるグリコシダーゼ活性検出試薬としては、例えば、フェノールフタレイン等のフェノール系酸塩基指示薬、5-ブロモ-4-クロロ-3-ヒドロキシインドール、5-ブロモ-6-クロロ-3-ヒドロキシインドール、6-クロロ-3-ヒドロキシインドール、3-ヒドロキシインドール等のヒドロキシインドール類縁体、4-メチルウンベリフェロン等のウンベリフェロン類縁体をアグリコン（非糖部分）とするグリコシド化合物を挙げることができる。 Examples of the glycosidase activity detection reagent that generates a dye compound or a fluorescent dye compound that is hardly soluble in water after the enzyme reaction include, for example, phenolic acid-base indicators such as phenolphthalein, 5-bromo-4-chloro-3-hydroxyindole, 5 Hydroxylindole analogues such as bromo-6-chloro-3-hydroxyindole, 6-chloro-3-hydroxyindole, 3-hydroxyindole, and umbelliferone analogues such as 4-methylumbelliferone And a glycoside compound as (part).
本発明で使用するグリコシダーゼ活性検出試薬は、上述のアグリコンと糖とがβ-D-グリコシド結合により結合したグリコシド化合物がより好ましい。 The glycosidase activity detection reagent used in the present invention is more preferably a glycoside compound in which the above-mentioned aglycone and sugar are bound by a β-D-glycoside bond.
更に、発色のpH安定性の観点からヒドロキシインドール類縁体のグリコシド化合物、ウンベリフェロン類縁体のグリコシド化合物が好ましく、また、これらのうち、酵素反応により生じる化合物が可視領域に吸収を持つ色素化合物であり、これが水に特に難溶でもある観点から、ヒドロキシインドール類縁体のグリコシド化合物が特に好ましい。 Furthermore, from the viewpoint of pH stability of color development, hydroxyindole analog glycoside compounds and umbelliferone analog glycoside compounds are preferred, and among these compounds, compounds produced by enzymatic reactions are dye compounds that absorb in the visible region. In view of the fact that it is particularly hardly soluble in water, a hydroxyindole analog glycoside compound is particularly preferred.
これらのグリコシダーゼ活性検出試薬は糖部位の違いにより、種々のグリコシダーゼ活性を検出するものであり、例えば、グルコース、グルクロン酸、マンノース、ガラクトース、フルクトース、フコース、ラムノース、アラビノース、キシロースを糖部位として有するものを挙げることができる。この中でも尿臭関連微生物酵素の検出性の観点からグルコース、グルクロン酸、ガラクトースが前述のアグリコンと結合したものが好ましく、更にこれらの中でも尿臭気発生との関わりからグルクロン酸と前述のアグリコンのβ-D-グリコシド結合により成る化合物、即ちβ-グルクロニダーゼ活性検出試薬が特に好ましい。 These glycosidase activity detection reagents detect various glycosidase activities depending on the sugar moiety, such as glucose, glucuronic acid, mannose, galactose, fructose, fucose, rhamnose, arabinose, and xylose as sugar moieties. Can be mentioned. Of these, glucose, glucuronic acid, and galactose combined with the above-mentioned aglycone are preferable from the viewpoint of detectability of urine odor-related microbial enzymes, and among these, β- A compound comprising a D-glycoside bond, that is, a β-glucuronidase activity detection reagent is particularly preferred.
このようなものとして具体的には、ヒドロキシインドール類縁体のグルクロン酸化化合物として5-ブロモ-4-クロロ-3-インドリル-β-D-グルクロニド、5-ブロモ-6-クロロ-3-インドリル-β-D-グルクロニド、6-クロロ-3-インドリル-β-D-グルクロニド、インドキシル-β-D-グルクロニドを、ウンベリフェロン類縁体のグルクロン酸化化合物としては4-メチルウンベリフェリル-β-D-グルクロニドを挙げることができる。 Specific examples of such compounds include 5-bromo-4-chloro-3-indolyl-β-D-glucuronide, 5-bromo-6-chloro-3-indolyl-β as the hydroxyindole analog glucuronidated compounds. -D-glucuronide, 6-chloro-3-indolyl-β-D-glucuronide, indoxyl-β-D-glucuronide, and 4-methylumbelliferyl-β-D as the umbelliferone analog glucuronidation compound -Glucuronide can be mentioned.
また、これらのうち、酵素反応により生じる化合物が可視領域に吸収を持つ色素化合物であり、これが水に特に難溶でもある観点から、ヒドロキシインドール類縁体のグルクロン酸化化合物、即ち、5-ブロモ-4-クロロ-3-インドリル-β-D-グルクロニド、5-ブロモ-6-クロロ-3-インドリル-β-D-グルクロニド、6-クロロ-3-インドリル-β-D-グルクロニド、インドキシル-β-D-グルクロニドがより好ましく、以上の中でも色素化合物の尿色とのコントラスト、発色の安定性及び価格の観点から5-ブロモ-4-クロロ-3-インドリル-β-D-グルクロニドが特に好ましい。 Of these, the compound produced by the enzyme reaction is a dye compound having absorption in the visible region, and from the viewpoint that it is particularly hardly soluble in water, a hydroxyindole analog glucuronidated compound, that is, 5-bromo-4 -Chloro-3-indolyl-β-D-glucuronide, 5-bromo-6-chloro-3-indolyl-β-D-glucuronide, 6-chloro-3-indolyl-β-D-glucuronide, indoxyl-β- D-glucuronide is more preferred, and among these, 5-bromo-4-chloro-3-indolyl-β-D-glucuronide is particularly preferred from the viewpoint of contrast with the urine color of the dye compound, stability of color development and cost.
＜サルファターゼ活性検出試薬＞
本発明に用いられるサルファターゼ活性検出試薬は、酵素反応後に水に難溶の色素化合物又は蛍光色素化合物を生じる酵素基質型のサルファターゼ活性検出試薬であって、サルファターゼ活性を視覚的に検出できるものであれば特に限定されない。また、尿臭気発生との関わりからアリールサルファターゼ活性検出試薬がより好ましい。
<Sulphatase activity detection reagent>
The sulfatase activity detection reagent used in the present invention is an enzyme substrate type sulfatase activity detection reagent that generates a dye compound or a fluorescent dye compound that is hardly soluble in water after an enzyme reaction, and can detect sulfatase activity visually. If it is a thing, it will not specifically limit. In addition, an arylsulfatase activity detection reagent is more preferable in relation to generation of urine odor.
以下、本発明において用いられるアリールサルファターゼ活性検出試薬について詳述するが、本発明において用いられるアリールサルファターゼ活性検出試薬は上述のような性状を有するものであれば特に限定されない。 Hereinafter, the arylsulfatase activity detection reagent used in the present invention will be described in detail, but the arylsulfatase activity detection reagent used in the present invention is not particularly limited as long as it has the above-described properties.
本発明において用いられるアリールサルファターゼ活性検出試薬としてはヒドロキシインドール類縁体の硫酸化化合物、ウンベリフェロン類縁体の硫酸化化合物を挙げることができる。 Examples of the arylsulfatase activity detection reagent used in the present invention include a sulfated compound of a hydroxyindole analog and a sulfated compound of an umbelliferone analog.
この中でも、ヒドロキシインドール類縁体の硫酸化化合物としては5-ブロモ-4-クロロ-3-インドリルサルフェイト、インドキシルサルフェイトを、ウンベリフェロン類縁体の硫酸化化合物としては4-メチルウンベリフェリルサルフェイトを、それぞれ好適に用いることができる。 Among them, 5-bromo-4-chloro-3-indolyl sulfate and indoxyl sulfate are used as sulfated compounds for hydroxyindole analogs, and 4-methylumbellite is used as a sulfated compound for umbelliferone analogs. Ferryl sulfate can be suitably used for each.
また、これらのうち、酵素反応により生じる化合物が可視領域に吸収を持つ色素化合物であって、これが水に特に難溶でもある観点から、ヒドロキシインドール類縁体の硫酸化化合物、即ち、5-ブロモ-4-クロロ-3-インドリルサルフェイト、インドキシルサルフェイトがより好ましく、色素化合物の尿色とのコントラスト、発色の安定性及び価格の観点から5-ブロモ-4-クロロ-3-インドリルサルフェイトが特に好ましい。 Of these, the compound produced by the enzymatic reaction is a dye compound having absorption in the visible region, and from the viewpoint that it is particularly hardly soluble in water, a sulfated compound of a hydroxyindole analog, that is, 5-bromo- 4-Chloro-3-indolylsulfate and indoxylsulfate are more preferable, and 5-bromo-4-chloro-3-indolylsulfate from the viewpoint of contrast with the urine color of the coloring compound, stability of color development and price Fate is particularly preferred.
＜酵素活性検出試薬の量、吸収性構造体及び吸収材への配合方法＞
本発明において吸収性構造体中の酵素活性検出試薬の好ましい含有量は、当該吸収性構造体である吸収性物品、当該吸収性構造体が使用される吸収性物品の吸収容量によって異なる。
具体的には、吸収性物品の吸収容量に対し、対イオンを除いた換算重量として、０．０００５ｗ／ｖ％以上とすることが好ましく、より好ましくは０．００１２５ｗ／ｖ％以上、更に好ましくは０．００２５ｗ／ｖ％以上であり、また、０．２ｗ／ｖ％以下とすることが好ましく、より好ましくは０．０５ｗ／ｖ％以下、更に好ましくは０．０２５ｗ／ｖ％以下であり、例えば、０００５〜０．２ｗ／ｖ％とすることが好ましく、より好ましくは０．００１２５〜０．０５ｗ／ｖ％であり、更に好ましくは０．００２５〜０．０２５ｗ／ｖ％である。
例えば、吸収性物品が吸収容量４００ｍＬの尿取りパッドであれば、１製品当りの酵素活性検出試薬の配合量は対イオンを除いた換算重量として２ｍｇ〜８００ｍｇとするのが良く、より好ましくは５ｍｇ〜２００ｍｇであり、更に好ましくは１０ｍｇ〜１００ｍｇである。
吸収性物品の吸収容量は、市販の吸収性物品の包装や説明に各メーカーが定めたものが記載されている場合等、その製品について定められている値がある場合には、その値を採用することが好ましい。吸収容量が定められていないものであれば、最大吸収量を吸収容量と規定することができる。なお、吸収性物品の最大吸収量は下記のようにして測定される。
<Amount of enzyme activity detection reagent, absorptive structure and blending method into absorbent material>
In the present invention, the preferable content of the enzyme activity detection reagent in the absorbent structure varies depending on the absorbent article that is the absorbent structure and the absorption capacity of the absorbent article in which the absorbent structure is used.
Specifically, the converted weight excluding counter ions is preferably 0.0005 w / v% or more, more preferably 0.00125 w / v% or more, still more preferably, with respect to the absorption capacity of the absorbent article. 0.0025 w / v% or more, preferably 0.2 w / v% or less, more preferably 0.05 w / v% or less, still more preferably 0.025 w / v% or less. 0005 to 0.2 w / v%, more preferably 0.00125 to 0.05 w / v%, and still more preferably 0.0025 to 0.025 w / v%.
For example, if the absorbent article is a urine picking pad with an absorption capacity of 400 mL, the blending amount of the enzyme activity detection reagent per product should be 2 mg to 800 mg as a converted weight excluding counter ions, more preferably 5 mg. It is -200 mg, More preferably, it is 10 mg-100 mg.
Absorption capacity of absorbent products is adopted when there is a value specified for the product, such as when the manufacturer's specifications are described in the packaging and description of commercially available absorbent products. It is preferable to do. If the absorption capacity is not defined, the maximum absorption amount can be defined as the absorption capacity. In addition, the maximum absorption amount of an absorbent article is measured as follows.
吸収性物品を２０℃の生理食塩水に３０分間浸漬し、３０分経過後吸収性物品を引き上げ、水切りをするのに充分な大きさの網目を有する金属製のふるい上で３０分間水切りを行い、水切り後の重量（ｇ）から浸漬前の重量（ｇ）を差し引き、この値を生理食塩水の比重（1.006ｇ/mL：20℃）で除した値を最大吸収量とする。
また、例えば吸水性ポリマーを主たる吸収材とする吸収性物品については、吸水性ポリマー質量の３０倍を吸収容量と仮定して配合量等を決めてもよい。
The absorbent article is soaked in physiological saline at 20 ° C. for 30 minutes, and after 30 minutes, the absorbent article is pulled up and drained for 30 minutes on a metal sieve having a mesh size sufficient for draining. Subtract the weight (g) before soaking from the weight (g) after draining, and divide this value by the specific gravity of physiological saline (1.006 g / mL: 20 ° C.).
For example, for an absorbent article mainly composed of a water-absorbing polymer, the blending amount and the like may be determined on the assumption that the absorbent capacity is 30 times the mass of the water-absorbing polymer.
また、本発明において吸収性構造体は、市販の製品に限らず、市販品を模した試作品、又は製品もしくは試作品の切片であっても良い。この場合、好適な酵素活性検出試薬の量は、元となる製品と対象となる試作品又は切片の吸収容量の比が、元となる製品と対象となる試作品又は切片の面積比と等しいものとして算出されたものであることが好ましい。
また、本発明において吸収材に保持させる酵素活性検出試薬の量は、吸収材の重量に対し、対イオンを除いた換算重量として０．０６〜６ｗ／ｗ％とするのが良く、より好ましくは０．１５〜１．５ｗ／ｗ％であり、更に好ましくは０．３〜０．７５ｗ／ｗ％である。
また、本発明において吸収材に保持させる酵素活性検出試薬の量は、吸収材の吸収容量に対して０００５〜０．２ｗ／ｖ％とすることが好ましく、より好ましくは０．００１２５〜０．０５ｗ／ｖ％であり、更に好ましくは０．００２５〜０．０２５ｗ／ｖ％である。
In the present invention, the absorbent structure is not limited to a commercially available product, but may be a prototype imitating a commercially available product, or a product or a section of a prototype. In this case, the suitable amount of enzyme activity detection reagent is such that the ratio of the absorption capacity of the original product to the target prototype or section is equal to the area ratio of the original product to the target prototype or section. It is preferable that it is calculated as.
In the present invention, the amount of the enzyme activity detection reagent to be retained in the absorbent material is preferably 0.06 to 6 w / w% as a converted weight excluding the counter ion with respect to the weight of the absorbent material, and more preferably. It is 0.15-1.5 w / w%, More preferably, it is 0.3-0.75 w / w%.
In the present invention, the amount of the enzyme activity detection reagent held in the absorbent material is preferably 0005 to 0.2 w / v%, more preferably 0.00125 to 0.05 w, based on the absorption capacity of the absorbent material. / V%, more preferably 0.0025 to 0.025 w / v%.
本発明において酵素活性検出試薬を、吸収性物品又はその吸収性コアに含有させる方法は、結果的に、吸収性物品又はその吸収性コア内に配合される方法であればどのような方法でも構わない。また、本発明において酵素活性検出試薬を、吸収材に保持させる方法は、結果的に、吸収材に保持される方法であればどのような方法でも構わない。
例えば、酵素活性検出試薬を、これを粉体のまま吸収性物品又はその吸収性コアに配合しても良いし、吸収性物品や吸収性コアの全体により均一に配合させる目的で、これを各種溶媒に溶解あるいは分散させた液剤を含浸させた後、乾燥工程により溶媒を揮散させて配合しても良い。
また、吸水性ポリマー等の吸収材にも、溶解あるいは分散させた液剤を接触させた後、乾燥することで、吸収材にも酵素活性検出試薬を効率よく保持させることができる。
In the present invention, the method for incorporating the enzyme activity detection reagent into the absorbent article or the absorbent core thereof may be any method as long as the method is incorporated into the absorbent article or the absorbent core as a result. Absent. In the present invention, the enzyme activity detection reagent may be retained on the absorbent material as a result of any method as long as it is retained on the absorbent material.
For example, the enzyme activity detection reagent may be blended with the absorbent article or the absorbent core in the form of a powder, or for the purpose of uniformly blending with the entire absorbent article or absorbent core. After impregnating with a solution dissolved or dispersed in a solvent, the solvent may be volatilized by a drying process.
In addition, the enzyme activity detection reagent can be efficiently retained in the absorbent material by bringing the dissolved or dispersed liquid into contact with the absorbent material such as a water-absorbing polymer and then drying.
溶媒に酵素活性検出試薬を溶解、分散させた液剤を含浸させた後、乾燥させて酵素活性検出試薬を配合する場合、その液剤は、吸収性物品や吸収性コア自体に含浸させても良いし、これを構成する個々の部材、即ち吸水性ポリマー、不織布、パルプ、台紙等それぞれに液剤を含浸させ、これら部材を用いて物品を作製しても良い。
また、上述のように乾燥工程を必要とする場合、使用する溶媒は、揮発性を有する有機溶剤又は水であることが好ましい。また、揮発性の有機溶剤の中でも水溶性を有するものが好ましく、たとえばメタノール、エタノール、1-プロパノール、2-プロパノール、アセトニトリル等を挙げることができる。これらの有機溶剤および水は単独で用いても良いし、任意の割合で混合して用いても良い。
また、さらには必要に応じて種々の界面活性剤、酸化防止剤、pH調整剤、油剤、有機溶剤、金属塩、金属イオン類等を、本発明の効果を阻害しない範囲で含有し、酵素活性検出試薬と共に吸収性構造体又は吸収材に固定化しても良い。
When the enzyme activity detection reagent is impregnated with a solvent in which the enzyme activity detection reagent is dissolved and dispersed, and then dried and blended with the enzyme activity detection reagent, the solution may be impregnated in the absorbent article or the absorbent core itself. The individual members constituting this, that is, the water-absorbing polymer, the nonwoven fabric, the pulp, the mount, etc., may be impregnated with a liquid agent, and an article may be produced using these members.
Moreover, when a drying process is required as mentioned above, it is preferable that the solvent to be used is a volatile organic solvent or water. Further, among the volatile organic solvents, those having water solubility are preferable, and examples thereof include methanol, ethanol, 1-propanol, 2-propanol, acetonitrile and the like. These organic solvents and water may be used alone, or may be mixed and used at an arbitrary ratio.
Furthermore, if necessary, it contains various surfactants, antioxidants, pH adjusters, oils, organic solvents, metal salts, metal ions, etc. as long as they do not impair the effects of the present invention, and enzyme activity You may fix | immobilize to an absorptive structure or an absorber with a detection reagent.
＜尿臭発生部の可視化及び特定方法＞
本発明の吸収性構造体又は吸収材を用いて、吸収性構造体等における尿臭発生部を特定するには、吸収性構造体としての吸収性物品や吸収性コア、吸収材を含む尿臭発生部を特定したい対象物等に、例えば、下記の成分１〜４より選ばれる何れか一以上を含有する液剤を添加することが好ましい。また、本液剤は必要に応じて水、生理食塩水、各種ｐＨ調整剤を含む緩衝液等の水溶液あるいはこれらの混合液で希釈して用いても良い。
成分１：酵素活性検出試薬に作用し色素化合物又は蛍光色素化合物を生じる酵素
成分２：酵素活性検出試薬に作用し色素化合物又は蛍光色素化合物を生じる酵素を有する細菌その他の微生物
成分３：ヒト又は動物の尿
成分４：酵素あるいは微生物の賦活剤
成分４の酵素あるいは微生物の賦活剤は微生物の増殖を促し、あるいは酵素反応を円滑に進ませる目的で用いられるものである。本賦活剤はこの目的に適うものであればどのようなものでも良いが、具体的には肉エキス、酵母エキス、カゼイン分解物、アミノ酸、糖、多糖、ビタミン、無機塩類及びｐＨ調整剤等より選ばれる成分、あるいはこれら成分を含むＳＣＤ培地、ミュラーヒントン培地、ＬＢ培地等の汎用の栄養培地が好ましい。
<Visualization and identification method of urine odor generating part>
In order to specify the urine odor generating part in the absorbent structure or the like using the absorbent structure or the absorbent material of the present invention, the urine odor containing the absorbent article, the absorbent core, and the absorbent material as the absorbent structure It is preferable to add, for example, a liquid agent containing any one or more selected from the following components 1 to 4 to an object or the like whose generation part is desired to be specified. Moreover, this liquid agent may be used by diluting with water, physiological saline, an aqueous solution such as a buffer solution containing various pH adjusting agents, or a mixture thereof, as necessary.
Component 1: An enzyme that acts on an enzyme activity detection reagent to produce a dye compound or fluorescent dye compound Component 2: A bacterium or other microorganism having an enzyme that acts on an enzyme activity detection reagent to produce a dye compound or fluorescent dye compound Component 3: Human or animal Component 4: Enzyme or microbial activator Component 4 enzyme or microbial activator is used for the purpose of promoting the growth of microorganisms or allowing the enzymatic reaction to proceed smoothly. The activator may be anything as long as it is suitable for this purpose. Specifically, from meat extract, yeast extract, casein degradation product, amino acid, sugar, polysaccharide, vitamin, inorganic salt, pH adjuster, etc. Preferred components or general-purpose nutrient media such as SCD medium, Muller Hinton medium, and LB medium containing these components are preferred.
本発明の吸収性構造体又は吸収材に対し、成分１〜４より選ばれる何れか一以上を含有する液剤を添加し、上記の酵素が働く適宜の温度、好ましくはその酵素の最適温度付近の温度に所定時間維持することにより、酵素活性検出試薬から水に難溶の色素化合物又は蛍光色素化合物が生じて発色する。そのとき、発色した部位、特に発色が強い部位は、臭気成分前駆体を分解して臭気成分を生じる酵素の酵素活性が高い部位であるため、実際に使用した場合においても尿臭が発生し易い尿臭発生部である。 A liquid agent containing any one or more selected from components 1 to 4 is added to the absorbent structure or absorbent material of the present invention, and an appropriate temperature at which the enzyme works, preferably around the optimum temperature of the enzyme. By maintaining the temperature for a predetermined time, a dye compound or fluorescent dye compound hardly soluble in water is generated from the enzyme activity detection reagent, and color is developed. At that time, the colored portion, particularly the strong colored portion, is a portion where the enzyme activity of the enzyme that decomposes the odor component precursor to generate the odor component is high, and thus urine odor is likely to be generated even in actual use. It is a urine odor generating part.
このように、本発明の吸収性構造体又は吸収材によれば、尿臭発生部を簡便且つ容易に視覚的に特定することができる。
本発明の吸収性構造体である吸収性物品やその吸収性コアは、尿臭生成抑制効果に優れた吸収性物品を開発する際、あるいは尿臭生成抑制効果に優れた吸収性物品であることを消費者等に視覚的にアピールする際に有用であり、また、一般消費者が使用する市販品や該市販品に組み込む吸収性コアとすることもできる。例えば、部位が特定された尿臭発生部に、消臭剤、芳香剤、抗菌剤、酵素阻害剤を優先的に配合すれば、尿臭生成抑制効果に優れた吸収性物品とすることができ、また、消臭剤、芳香剤、抗菌剤、酵素阻害剤の量や部位を変更しつつ、視覚的に特定された尿臭発生部の変化を観察すれば、消臭剤、芳香剤、抗菌剤、酵素阻害剤の好ましい配合量や配合部位を容易に決定できる。また、本発明の吸収材も、吸収性物品やその吸収性コア等の尿臭発生部を特定したい対象物に分散配置することにより、当該対象物の尿臭発生部を簡便且つ客観的に特定することができる。
Thus, according to the absorptive structure or absorbent material of the present invention, the urine odor generating part can be visually identified easily and easily.
The absorbent article that is the absorbent structure of the present invention and the absorbent core thereof are absorbent articles that are excellent in the urine odor generation suppression effect or when developing an absorbent article that is excellent in the urine odor generation suppression effect. Is useful for visually appealing to consumers and the like, and can also be a commercial product used by general consumers or an absorbent core incorporated in the commercial product. For example, if a deodorant, a fragrance, an antibacterial agent, and an enzyme inhibitor are preferentially blended into the urine odor generating part whose site is specified, an absorbent article excellent in urine odor generation suppressing effect can be obtained. In addition, if the change in the visually identified urine odor generation part is observed while changing the amount and site of the deodorant, fragrance, antibacterial agent, enzyme inhibitor, the deodorant, fragrance, antibacterial The preferable blending amount and blending site of the agent and the enzyme inhibitor can be easily determined. In addition, the absorbent material of the present invention can be easily and objectively specified by allocating the urine odor generating part of the target object by dispersing and arranging the urine odor generating part such as the absorbent article or the absorbent core on the target object. can do.
また、上述の用途に応じて、成分１〜４の好適な組合せは異なる。
本発明の吸収性構造体又は吸収材は尿の有無に関わらず試薬の呈色によって尿臭発生部を模擬的に示すことができる点にも特徴を有するものであり、たとえば微生物や尿を含むサンプルが好まれない場面においては、成分１を含んで成る液剤を適用することが好ましく、必要に応じてさらに成分４を含んでも良い。
また、製品の実使用調査等においてこれを用いる場合、使用者あるいはペット等の排尿行為によって成分３が適用される。また、このとき酵素反応は製品の使用時に使用者から移行した微生物あるいは環境に常在の微生物等に由来する酵素よって進行させることが可能である。
一方、より再現性良く酵素反応を行いたい場合や、対象が新品の製品、素材等、無菌あるいはほとんどの微生物が除去された状態となっている場合においては、成分１あるいは成分２を含んで成る液剤を適用することが好ましく、より好ましくはさらに成分３及び／あるいは成分４、特には成分３を含んで成る液剤を適用することが好ましい。また、このとき再現性の観点から、成分３は除菌されたものであることが好ましい。
Moreover, the suitable combination of the components 1-4 changes with the above-mentioned uses.
The absorbent structure or absorbent material of the present invention is also characterized in that the urine odor generating part can be simulated by coloration of the reagent regardless of the presence or absence of urine, and includes, for example, microorganisms and urine In the case where the sample is not preferred, it is preferable to apply a liquid preparation containing component 1, and may further include component 4 as necessary.
Moreover, when using this in the actual use survey of a product etc., the component 3 is applied by the urination act of a user or a pet. At this time, the enzyme reaction can be caused to proceed by an enzyme derived from a microorganism transferred from the user at the time of use of the product or a microorganism resident in the environment.
On the other hand, when the enzyme reaction is desired to be performed with higher reproducibility, or when the target is aseptic or most of microorganisms are removed, such as a new product or material, it contains component 1 or component 2. It is preferable to apply a liquid agent, and it is more preferable to apply a liquid agent comprising component 3 and / or component 4, particularly component 3. At this time, from the viewpoint of reproducibility, component 3 is preferably sterilized.
参考例１ 酵素活性検出試薬の選択
図３に示すように、吸水性ポリマー0.03ｇに表１に示す組成の酵素活性検出試薬又は臭気感色性指示薬を含有する各液剤300μLを吸収させ、これを更に、1.5mL容マイクロテストチューブ内において間にパルプ層を挟み３層に積層させた。この中央の層にのみ、臭気発生のイニシエーターとなる酵素、あるいは細菌を添加し、37℃恒温槽中で72時間臭気発生を行い、色の変化を観察した。この結果を表２及び図４に示す。表２には、発色性、尿色に対するコントラスト、尿臭発生部の特定の正確さについて、４名のパネルが以下の基準に基づき、協議によって判定した結果を示す。
Reference Example 1 Selection of Enzyme Activity Detection Reagent As shown in FIG. 3, 300 μL of each solution containing the enzyme activity detection reagent or odor color indicator of the composition shown in Table 1 was absorbed in 0.03 g of a water-absorbing polymer. Further, the pulp layer was sandwiched between three layers in a 1.5 mL micro test tube. Only this central layer was added with an enzyme or bacteria as an initiator of odor generation, and the odor was generated in a 37 ° C. constant temperature bath for 72 hours, and the color change was observed. The results are shown in Table 2 and FIG. Table 2 shows the results of discussion by four panels based on the following criteria for color development, contrast to urine color, and specific accuracy of the urine odor generating part.
＜発色性＞
ａ：強く発色する。
ｂ：やや強く発色する。
ｃ：発色が弱い。
ｄ：発色しない。
<Color development>
a: Strongly colored.
b: Strongly colored.
c: Color development is weak.
d: No color development.
＜尿色に対するコントラスト＞
ａ：尿と色素化合物の色のコントラストが強い。
ｂ：尿と色素化合物の色のコントラストがやや強い。
ｃ：尿と色素化合物の色のコントラストが弱い。
ｄ：尿と色素化合物の色の区別がつかない。
−：発色不良（発色性：ｄ）のため、評価不能。
<Contrast with urine color>
a: The color contrast between urine and the pigment compound is strong.
b: Slightly strong color contrast between urine and pigment compound.
c: Color contrast between urine and pigment compound is weak.
d: The color of urine and pigment compound cannot be distinguished.
−: Evaluation is impossible due to poor color development (color development: d).
＜臭気発生部の特定の正確さ＞
ａ：尿臭の発生した位置が明確にわかる。
ｂ：尿臭が発生した位置がわかる。
ｃ：尿臭が発生した位置がやや不明確である。
ｄ：尿臭が発生した位置が不明確である。
−：発色不良（発色性：ｄ）のため、評価不能。
<Specific accuracy of odor generating part>
a: The position where urine odor is generated can be clearly seen.
b: The position where urine odor is generated is known.
c: The position where the urine odor is generated is somewhat unclear.
d: The position where the urine odor is generated is unclear.
−: Evaluation is impossible due to poor color development (color development: d).
この結果から明らかなように、本発明で用いる水に難溶の色素化合物を生じる酵素基質型の酵素活性検出試薬を含有する液剤を吸収させたサンプル（参考例1-1〜1-7）は酵素、細菌のどちらをイニシエーターとして用いた場合にも中央の層のみで明瞭な色の変化が観察され、臭気発生部の明確な特定に成功した。これに対し臭気感色性指示薬を含有した比較例1-3及び酵素反応後の色素化合物が水溶性（4-ニトロフェノール水溶解度 11.6g/L（20℃））である比較例1-1では色の変化は見られたが、発色部位が拡散してしまい臭気発生部の特定には至らなかった。また比較例1-2、1-4のように、いくつかの臭気感色性指示薬は吸水性ポリマー中では指示薬として機能しなかった。 As is apparent from the results, samples (Reference Examples 1-1 to 1-7) in which a solution containing an enzyme substrate type enzyme activity detection reagent that produces a dye compound hardly soluble in water used in the present invention was absorbed (Reference Examples 1-1 to 1-7) When either enzyme or bacteria was used as the initiator, a clear color change was observed only in the middle layer, and the odor generation part was clearly identified. On the other hand, in Comparative Example 1-3 containing an odor color indicator and Comparative Example 1-1 in which the dye compound after the enzyme reaction is water-soluble (4-nitrophenol water solubility 11.6 g / L (20 ° C.)) Although a color change was observed, the colored portion diffused and the odor generating part could not be specified. Further, as in Comparative Examples 1-2 and 1-4, some odor color sensitive indicators did not function as indicators in the water-absorbing polymer.
実施例１ 尿取りパッドにおける尿臭発生部の可視化
（１）吸収体切片の調製
市販の尿取りパッドの構成を模倣して尿取りパッドを作製し、その中央部に近い位置から5.5cm四方を切り出し、この吸収体切片を以下の評価に供した。なお、このとき参照した市販尿取りパッドの吸収容量に基づいて算出した吸収体切片の吸収容量はおよそ18mLである。
（２）吸収体切片への酵素活性検出試薬の配合
エタノールに対し、5-ブロモ-4-クロロ-3-インドリル-β-D-グルクロニドを200ppm（w/v）溶解させることにより、酵素活性検出試薬を含有する液剤を調製した。得られた液剤12.5mLを前記（１）の吸収体切片に均一に含浸させ、電気乾燥機中で重量が変化しなくなるまで乾燥させた。
Example 1 Visualization of urine odor generating part in urine picking pad (1) Preparation of absorber section A urine picking pad was made by imitating the structure of a commercially available urine picking pad, and a 5.5 cm square was measured from a position close to the central part. It cut out and this absorber slice was used for the following evaluation. In addition, the absorption capacity of the absorber slice calculated based on the absorption capacity of the commercially available urine collection pad referred to at this time is approximately 18 mL.
(2) Mixing of enzyme activity detection reagent into the absorber section Detection of enzyme activity by dissolving 200 ppm (w / v) of 5-bromo-4-chloro-3-indolyl-β-D-glucuronide in ethanol A solution containing the reagent was prepared. 12.5 mL of the obtained liquid agent was uniformly impregnated into the absorber section of (1) above, and dried in an electric dryer until the weight did not change.
＜尿臭発生部の可視化の検証試験＞
（３）β-グルクロニダーゼ活性菌株の採取
使用済み（排尿のみ）の幼児用の使い捨ておむつから、5-ブロモ-4-クロロ-3-インドリル-β-D-グルクロニドを添加したSCDLP寒天平板培地（日本製薬株式会社製）を用いて常法によりβ-グルクロニダーゼ活性菌株の採取、分離を行った。これら菌株を除菌尿中で培養したところ臭気の発生が確認され、この中でも特に強い臭気を発生させた菌株を本実施例におけるβ-グルクロニダーゼ活性菌株として用いた。また本菌株は16S rDNA部分塩基配列解析によって大腸菌に帰属されるものであることを確認した。
（４）菌添加尿の調製
（３）に記載のβ-グルクロニダーゼ活性菌株をSCD寒天平板培地上で一晩培養し、得られたコロニーから一部を滅菌ループによってかき取り、除菌済み生理食塩水中に希釈してCFU/mLで104オーダーとなるように菌液を調製した。採取後すぐにナルゲン社製フィルターユニットにより除菌を行ったヒト除菌尿に、調製した菌液を１v/v％添加混合し、菌添加尿を調製した。
<Verification test for visualization of urine odor generating part>
(3) Collection of β-glucuronidase active strains SCDLP agar plate medium containing 5-bromo-4-chloro-3-indolyl-β-D-glucuronide from used disposable diapers (only for urination) (Japan) Β-glucuronidase active strains were collected and separated by a conventional method using a pharmaceutical company). Occurrence of odor was confirmed when these strains were cultured in sterilized urine. Among them, the strain that generated particularly strong odor was used as the β-glucuronidase active strain in this example. This strain was confirmed to be attributed to E. coli by 16S rDNA partial nucleotide sequence analysis.
(4) Preparation of fungus-added urine The β-glucuronidase active strain described in (3) is cultured overnight on a SCD agar plate medium, and a portion of the obtained colony is scraped off by a sterilization loop, and sterilized physiological saline the bacterial solution was prepared so as to be 10 4 order in CFU / mL was diluted in water. Immediately after collection, 1 v / v% of the prepared bacterial solution was added to and mixed with human sterilized urine sterilized using a filter unit manufactured by Nalgen to prepare urine added urine.
（５）酵素反応及び尿臭発生部の可視化
前記（１）で調製した吸収体切片に上面（図５）から、前記（４）で調製した菌添加尿を12.5mL滴下し、30℃恒温槽中において24時間静置した。
（６）専門パネルによる官能評価
このサンプルについて、初期、及び24時間後の臭気強度評価を行った。臭気強度評価には臭気判定士の資格を有する２名の専門パネルが携わり、０〜５の評価スコアによる６段階臭気強度表示法に準じて行った。即ち評価スコアは、「０」無臭、「１」やっと感知できるニオイ、「２」尿臭であることわかるが弱いニオイ、「３」楽に尿臭であると感じられるニオイ、「４」強い尿臭、「５」強烈な尿臭を示す。臭気強度の判定は0.5刻みで行い、２名の評価の平均値について、小数点以下の数値が、0.25である場合は0.5とし、0.75である場合は整数に切り上げた。
(5) Visualization of enzyme reaction and urine odor generating part 12.5 mL of the bacterium-added urine prepared in (4) above is dropped from the upper surface (FIG. 5) onto the absorbent section prepared in (1) above, and a 30 ° C. constant temperature bath It was allowed to stand for 24 hours.
(6) Sensory evaluation by specialized panel The odor intensity of this sample was evaluated at the initial stage and after 24 hours. Odor intensity evaluation was carried out in accordance with a 6-step odor intensity display method with an evaluation score of 0 to 5 involving two specialized panels with qualifications as odor judgers. That is, the evaluation score is “0” odorless, “1” odor that can be finally detected, “2” urine odor, but weak odor, “3” odor that feels urine easily, “4” strong urine odor "5" shows intense urine odor. The determination of odor intensity was performed in increments of 0.5, and the average value of the evaluation of the two persons was 0.5 when the numerical value after the decimal point was 0.25, and was rounded up to an integer when it was 0.75.
図６には（５）で得られた初期及び24時間後のサンプルの画像及び画像下には（６）で行った臭気強度評価値を示した。この結果は臭気判定士2名が行った官能評価の結果と非常に良く一致した。
本結果により本発明の吸収性物品により尿臭発生部を簡便に可視化できることが示された。
FIG. 6 shows the initial and 24 hour sample images obtained in (5) and the odor intensity evaluation values performed in (6) below the images. This result agrees very well with the results of sensory evaluation conducted by two odor judgeers.
This result shows that the urine odor generating part can be easily visualized by the absorbent article of the present invention.
実施例２ 使い捨ておむつの調製
エタノールに対し、5-ブロモ-4-クロロ-3-インドリル-β-D-グルクロニドを200ppm(w/v)溶解させることにより液剤を調製した。市販大人用使い捨ておむつ（リリーフ モレ安心・肌さらさらパンツ（尿吸収容量750mL））に本液剤450mLを均一に含浸させ、電気乾燥機中で重量が変化しなくなるまで乾燥させた。
実施例３ 吸水性ポリマーの調製
メタノールに対し、5-ブロモ-4-クロロ-3-インドリル-β-D-グルクロニドを2000ppm(w/v)溶解させることにより液剤を調製した。ナスフラスコ中で本液剤24mLと吸水性ポリマー8gを混合し、ロータリーエバポレーターによりメタノールを減圧留去した。
Example 2 Preparation of disposable diaper A solution was prepared by dissolving 200 ppm (w / v) of 5-bromo-4-chloro-3-indolyl-β-D-glucuronide in ethanol. A commercially available disposable diaper (Relief Mole Relief / Skin Smooth Pants (urine absorption capacity 750 mL)) was uniformly impregnated with 450 mL of this solution and dried in an electric dryer until the weight did not change.
Example 3 Preparation of water-absorbing polymer A solution was prepared by dissolving 2000 ppm (w / v) of 5-bromo-4-chloro-3-indolyl-β-D-glucuronide in methanol. In an eggplant flask, 24 mL of this solution and 8 g of a water-absorbing polymer were mixed, and methanol was distilled off under reduced pressure using a rotary evaporator.
実施例４ 軽失禁パッドの調製
実施例３で調製した吸水性ポリマー3gを他の部材（不織布、パルプ、台紙）と組み合わせて市販軽失禁パッド（中量用、尿吸収容量80mL）の構成を模倣した軽失禁パッドを作製した。
Example 4 Preparation of light incontinence pad Combining 3 g of the water-absorbing polymer prepared in Example 3 with other members (nonwoven fabric, pulp, mount) to mimic the structure of a commercially available light incontinence pad (medium amount, urine absorption capacity 80 mL) A light incontinence pad was made.
実施例２で調製した使い捨ておむつ及び実施例４で調製した軽失禁パッドのそれぞれについて、その中央部に近い位置から5.5cm四方を切り出した。これに対して、前記（４）で調製した菌添加尿を12.5mL滴下し、30℃恒温槽中において24時間静置した。
このサンプルについて図５に示した観察面を観察したところ、共に酵素反応に伴う臭気発生源の可視化に成功した。
About each of the disposable diaper prepared in Example 2, and the light incontinence pad prepared in Example 4, 5.5 cm square was cut out from the position near the center part. On the other hand, 12.5 mL of the urine added urine prepared in the above (4) was dropped and left in a constant temperature bath at 30 ° C. for 24 hours.
When the observation surface shown in FIG. 5 was observed for this sample, both succeeded in visualizing the odor generation source accompanying the enzyme reaction.
実施例５
メタノールに対し、5-ブロモ-4-クロロ-3-インドリルサルフェイトを200ppm（w/v）溶解させることにより、アリールサルファターゼ活性検出試薬を含有する液剤を調製した。得られた液剤12.5mLを実施例１の前記（１）と同様にして調製した吸収体切片に均一に含浸させ、ドラフトチャンバー内で重量が変化しなくなるまで乾燥させた。この切片に対して、アリールサルファターゼType VI（シグマ社より購入）を0.1M リン酸カリウム緩衝液（pH7.1）で希釈して0.04 units/mLとした酵素液12.5mLを滴下し、30℃恒温槽中において24時間静置した。
このサンプルについて図５に示した観察面を観察したところ、酵素反応に伴う青色の呈色反応が確認された。この結果から、アリールサルファターゼ活性検出試薬を用いた場合にも尿臭発生部を簡便に可視化できることが示された。
Example 5
A solution containing an arylsulfatase activity detection reagent was prepared by dissolving 200 ppm (w / v) of 5-bromo-4-chloro-3-indolylsulfate in methanol. 12.5 mL of the obtained liquid agent was uniformly impregnated into an absorbent section prepared in the same manner as in (1) of Example 1, and dried in a draft chamber until the weight did not change. To this section, 12.5 mL of an enzyme solution prepared by diluting arylsulfatase Type VI (purchased from Sigma) with 0.1 M potassium phosphate buffer (pH 7.1) to 0.04 units / mL was added dropwise at 30 ° C. It was allowed to stand for 24 hours in a thermostatic bath.
When the observation surface shown in FIG. 5 was observed for this sample, a blue color reaction accompanying the enzyme reaction was confirmed. From this result, it was shown that the urine odor generating part can be easily visualized even when the arylsulfatase activity detection reagent is used.
１ 吸収性物品（吸収性構造体）
２ 表面シート
３ 防漏シート
４ 吸収性コア
４２ パルプ繊維（繊維材料）
４３ 吸水性ポリマー
４４，４５ コアラップシート
1 Absorbent article (absorbent structure)
2 Surface sheet 3 Leak-proof sheet 4 Absorbent core 42 Pulp fiber (fiber material)
43 Water-absorbing polymer 44, 45 Core wrap sheet
Claims (6)
前記吸収性構造体は、吸収性物品又は該吸収性物品の吸収性コアであり、
前記吸収性物品が、軽失禁パッド、尿取りパッド、使い捨ておむつ、パンティーライナー、生理用ナプキン、タンポン、ペット用トイレ、又はペット用尿吸収シートである、吸収性構造体。 An absorptive structure containing an enzyme substrate type enzyme activity detection reagent that generates a dye compound or a fluorescent dye compound that is hardly soluble in water by an enzyme reaction by an enzyme that decomposes an odor component precursor to generate an odor component ,
The absorbent structure is an absorbent article or an absorbent core of the absorbent article,
An absorbent structure, wherein the absorbent article is a light incontinence pad, a urine picking pad, a disposable diaper, a panty liner, a sanitary napkin, a tampon, a pet toilet, or a pet urine absorbent sheet.
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