JP5976486B2 - Target surface treatment method - Google Patents

Target surface treatment method Download PDF

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JP5976486B2
JP5976486B2 JP2012221159A JP2012221159A JP5976486B2 JP 5976486 B2 JP5976486 B2 JP 5976486B2 JP 2012221159 A JP2012221159 A JP 2012221159A JP 2012221159 A JP2012221159 A JP 2012221159A JP 5976486 B2 JP5976486 B2 JP 5976486B2
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target surface
protein
inorganic particles
antibacterial
protamine
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JP2013100263A (en
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英明 成澤
英明 成澤
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MEDICAL LIFE QUALITY MEDICAL CORPORATION
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/28—Materials for coating prostheses
    • A61L27/30—Inorganic materials
    • A61L27/306—Other specific inorganic materials not covered by A61L27/303 - A61L27/32
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
    • C01B33/00—Silicon; Compounds thereof
    • C01B33/113—Silicon oxides; Hydrates thereof
    • C01B33/12—Silica; Hydrates thereof, e.g. lepidoic silicic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/18—Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Inorganic Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Materials For Medical Uses (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Other Surface Treatments For Metallic Materials (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)

Description

本発明は、金属、セラミックス等の耐熱性を有する対象表面に抗菌性を持たせたり、あるいは抗菌性を有する対象表面の抗菌性を一旦除去し再度抗菌性を持たせるための対象表面の処理方法に関するものである。   The present invention relates to a method for treating a target surface for imparting antibacterial properties to a target surface having heat resistance, such as metal or ceramics, or once removing the antibacterial properties of the target surface having antibacterial properties. It is about.

現在、歯ブラシのような日用品を代表として、さまざまな物品の表面に抗菌性を付与する技術が開示されている。   Currently, techniques for imparting antibacterial properties to the surfaces of various articles have been disclosed, with daily goods such as toothbrushes as representatives.

ここで、金属、セラミックス等の耐熱性を有する素材は、そのままでは抗菌性を付与することが困難である。そこで、特許文献１（米国公開２００４／７４５６８号公報）は、まず対象表面（チタン、チタン合金等）にアンカーモジュールを固定し、さらに、このアンカーモジュールを介して、バンコマイシン等の抗生物質を結合させる技術を開示する。   Here, it is difficult to impart antibacterial properties to heat-resistant materials such as metals and ceramics as they are. Therefore, Patent Document 1 (US Publication No. 2004/74568) first fixes an anchor module to a target surface (titanium, titanium alloy, etc.), and further binds an antibiotic such as vancomycin through the anchor module. Disclose technology.

このようにすれば、通常では抗菌性を付与しにくい対象表面に、抗菌性を付与することは可能であろう。   In this way, it would be possible to impart antibacterial properties to a target surface that is usually difficult to impart antibacterial properties.

ところが、従来技術では、いずれも抗菌性を付与することのみに終始しており、一旦付与した抗菌性を取り除いたり、再度付与し直して抗菌作用を再度活性化するための技術は知られていない。このように、一旦抗菌性を取り除いたり、再付与することは、例えば、歯科インプラントに使用される金属部品をメンテナンスする場合や、使用によりやや古くなり抗菌性が弱くなった台所用品、流し台の部品、あるいは冷蔵庫の内面等の技術分野では、高い有用性が見込まれるが、現状では未だ対応ができていない。
米国公開２００４／７４５６８号公報 However, in the prior art, all of them are only provided with antibacterial properties, and there is no known technique for reactivating the antibacterial action by removing the once imparted antibacterial properties or reapplying them. . In this way, once antibacterial properties have been removed or reapplied, for example, when maintaining metal parts used in dental implants, kitchenware and sink parts that have become slightly outdated and weakened in antibacterial properties. In the technical field such as the inner surface of a refrigerator, it is expected to be highly useful, but at present, no response has been made.
US Publication No. 2004/74568

そこで本発明は、対象表面に抗菌性を付与できるだけでなく、一旦付与された抗菌性を除去したり、あるいは除去後再度抗菌性を付与することができる対象表面の処理方法を提供することを目的とする。   Therefore, the present invention has an object to provide a method for treating a target surface that can not only provide antibacterial properties to a target surface, but also remove the antibacterial properties that have been once imparted, or reapply antibacterial properties after removal. And

第１の発明に係る対象表面の抗菌処理方法は、対象表面に無機粒子を結合させる工程と、無機粒子に、抗菌性を有するタンパク又はタンパクから誘導される塩基性ペプチドを結合させる工程と、対象表面を酸性環境とし、タンパク又は塩基性ペプチドを無機粒子から除去する工程とを含む。   An antibacterial treatment method for a target surface according to a first invention includes a step of binding inorganic particles to the target surface, a step of binding a protein having antibacterial properties or a basic peptide derived from a protein to the inorganic particles, and a target And a step of removing the protein or the basic peptide from the inorganic particles by setting the surface to an acidic environment.

第２の発明に係る対象表面の抗菌処理方法では、第１の発明に加え、タンパク又は塩基性ペプチドを除去した対象表面を中性又はアルカリ性環境とし、タンパク又は塩基性ペプチドを無機粒子に結合させる工程とをさらに含む。   In the antibacterial treatment method for the target surface according to the second invention, in addition to the first invention, the target surface from which the protein or basic peptide has been removed is made a neutral or alkaline environment, and the protein or basic peptide is bound to the inorganic particles. A process.

第３の発明に係る対象表面の抗菌処理方法では、第１の発明に加え、無機粒子は、シラノール基が露出するシリカ粒子である。   In the antibacterial treatment method for a target surface according to the third invention, in addition to the first invention, the inorganic particles are silica particles in which silanol groups are exposed.

第４の発明に係る対象表面の抗菌処理方法では、第１の発明に加え、タンパクは、魚類の白子由来のタンパクである。   In the antibacterial treatment method for a target surface according to the fourth invention, in addition to the first invention, the protein is a protein derived from a fish larva.

本発明によれば、対象表面に抗菌性を付与するだけでなく、一旦付与された抗菌性を除去したり、除去後再度抗菌性を付与することができる。その結果、歯科インプラント用の金属部品、台所用品、流し台の部品、あるいは冷蔵庫の内面等の技術分野において、高い有用性を発揮できる。   According to the present invention, not only antibacterial properties can be imparted to the target surface, but also the antibacterial properties once imparted can be removed or antibacterial properties can be imparted again after removal. As a result, high utility can be exhibited in technical fields such as metal parts for dental implants, kitchen utensils, sink parts, or the inner surface of a refrigerator.

次に、図面を参照しながら、本発明の実施の形態を説明する。   Next, embodiments of the present invention will be described with reference to the drawings.

本形態では、無機粒子は、シラノール基が露出するシリカ粒子とし、タンパクは、サケの白子由来のプロタミンを主とするが、プルタミンに属するサルミン及びクルペインも同様に使用できるし、作用効果も同等である。   In this embodiment, the inorganic particles are silica particles from which silanol groups are exposed, and the protein is mainly protamine derived from salmon egg white, but salmine and krupain belonging to plutamine can be used in the same manner and have the same effect. is there.

対象表面は、歯科領域で良く使用されるチタン表面とするが、他の金属あるいはセラミックスにおいても、同様に適用できる。対象表面は、シラコート処理されるものであるが、２０〜３０℃程度の低温で処理できる場合には、金属あるいはセラミックスでなくとも、樹脂等の比較的熱に弱い材料も対象とすることができる。   The target surface is a titanium surface that is often used in the dental field, but can be similarly applied to other metals or ceramics. The target surface is to be treated with silacoat, but if it can be processed at a low temperature of about 20 to 30 ° C., it is possible to target a relatively heat-sensitive material such as a resin, even if it is not metal or ceramic. .

図１は、本発明の一実施の形態における対象表面の拡大写真であり、無加工状態を示す。無加工のチタン表面に欠陥があると、欠陥部分が鋭利にえぐれているのが観察できる。   FIG. 1 is an enlarged photograph of a target surface in one embodiment of the present invention, and shows an unprocessed state. If there is a defect on the unprocessed titanium surface, it can be observed that the defect portion is sharply removed.

図２は、本発明の一実施の形態における対象表面の拡大写真であり、シリカ粒子蒸着加工後の状態を示す。図１の状態から、シリカ粒子を対象表面に蒸着加工により固定する。なお、シリカ粒子を対象表面に固定できさえすれば、蒸着加工ではない他の常法を使用しても良い。例えば、サンドブラスト法によりシリカ粒子を対象表面に固定しても良い。このようにすると、蒸着加工によるよりも多少効率が低下するが、操作がより簡便となるという利点がある。   FIG. 2 is an enlarged photograph of the target surface in one embodiment of the present invention, showing a state after silica particle deposition processing. From the state of FIG. 1, silica particles are fixed to the target surface by vapor deposition. In addition, as long as the silica particles can be fixed to the target surface, other conventional methods other than vapor deposition may be used. For example, silica particles may be fixed to the target surface by sandblasting. In this way, the efficiency is somewhat lower than by vapor deposition, but there is an advantage that the operation is simpler.

図１と比べると明らかなように、対象表面には全体的に細かなシリカ粒子が付着している状態が観察できる。一方、図１と同様に、欠陥部分は鋭利にえぐれている点が観察できる。   As is clear from the comparison with FIG. 1, it is possible to observe a state in which fine silica particles are attached to the entire target surface. On the other hand, as in FIG. 1, it can be observed that the defect portion is sharply removed.

図３は、本発明の一実施の形態における対象表面の拡大写真であり、プロタミン付加後の状態を示す。プロタミンは、シリカ粒子のシラノール基と結合し、シリカ粒子が塩基性ペプチドに包囲されることになるため、欠陥部分の鋭利さがほとんどなくなっている点が観察できる。   FIG. 3 is an enlarged photograph of the target surface in one embodiment of the present invention, showing a state after addition of protamine. Since protamine binds to the silanol groups of the silica particles and the silica particles are surrounded by the basic peptide, it can be observed that the sharpness of the defective portion is almost eliminated.

図４は、本発明の一実施の形態における対象表面の拡大写真であり、プロタミン除去後の状態を示す。図３の状態から、次亜塩素酸溶液を付着させる処理を行うと、塩基性ペプチドが溶解し除去される。その結果、シリカ粒子は塩基性ペプチドに包囲される前の状態に戻り、欠陥部分の輪郭が再度鋭利になっている点が観察できる。言い換えれば、この処理により、付与されていた対象表面の抗菌性が除去されたことが分かる。   FIG. 4 is an enlarged photograph of the target surface in one embodiment of the present invention, showing a state after removal of protamine. If the process which makes a hypochlorous acid solution adhere from the state of FIG. 3, a basic peptide will melt | dissolve and will be removed. As a result, the silica particles return to the state before being surrounded by the basic peptide, and it can be observed that the outline of the defective portion is sharpened again. In other words, it can be seen that the antibacterial property of the applied target surface has been removed by this treatment.

図５は、本発明の一実施の形態における対象表面の拡大写真であり、プロタミン再付加後の状態を示す。図４の状態では、対象表面に結合するシリカ粒子は、図２の状態に戻ったことになり、シリカ粒子は、対象表面に結合したまま除去されない状態にある。   FIG. 5 is an enlarged photograph of the target surface in one embodiment of the present invention, showing a state after re-addition of protamine. In the state of FIG. 4, the silica particles bonded to the target surface have returned to the state of FIG. 2, and the silica particles are in a state where they are not removed while being bonded to the target surface.

そして、適当な処理液を使用して、対象表面を中性あるいはアルカリ性の環境とした上で、再度プロタミンを付加すると、図５の状態となる。図５を図２と比べると共通性が理解されよう。即ち、塩基性ペプチドによりシリカ粒子が再び包囲され、欠陥部分の鋭利さが図２と同様になくなっている。   Then, when protamine is added again after making the target surface neutral or alkaline using an appropriate treatment solution, the state shown in FIG. 5 is obtained. Comparing FIG. 5 with FIG. 2, the commonality will be understood. That is, the silica particles are surrounded again by the basic peptide, and the sharpness of the defective portion disappears as in FIG.

以上の実験例により、本願発明が実施可能であることが理解されよう。ここで、歯科領域における本発明の意義を考慮すると、次のようになる。即ち、半埋め込み式医療材料、歯科金属は一度設置した場合除去は容易ではない。素材そのものに抗菌性を与えた場合、抗菌剤によるアレルギーの疑いにより再手術して除去せざるをえないが、本法によれば表面の抗菌成分を一度除去して確認した後、問題なければ再度抗菌性を与えることができ、実益大である。   It will be understood from the above experimental examples that the present invention can be implemented. Here, the significance of the present invention in the dental field is considered as follows. That is, semi-implantable medical materials and dental metals are not easy to remove once installed. If the material itself is given antibacterial properties, it must be removed by re-operation due to allergy to antibacterial agents, but according to this method, once the surface antibacterial components have been removed and confirmed, there is no problem. Antibacterial properties can be given again, which is a great benefit.

以下、図６を参照しながら、本形態の対象表面の処理方法の各工程を説明する。   Hereafter, each process of the processing method of the target surface of this form is demonstrated, referring FIG.

まず図６（ａ）に示すように、対象表面１を中性又はアルカリ性の状態として、外部に露呈させる。   First, as shown in FIG. 6A, the target surface 1 is exposed to the outside in a neutral or alkaline state.

次に、対象表面１にシラコート処理を施し、シリカ粒子３を対象表面１に固定する。また、各シリカ粒子１の表面には、シラノール基３が現れる。   Next, the target surface 1 is subjected to a silacoat treatment, and the silica particles 3 are fixed to the target surface 1. In addition, silanol groups 3 appear on the surface of each silica particle 1.

図６（ｂ）の状態となったら、対象表面１にプロタミン４を塗布すると、図６（ｃ）に示すように、シラノール基４にプロタミン４が結合し、シリカ粒子２は、プロタミン４に包囲させる。この後、口腔内等に対象表面１を有する部材を入れて使用しても、通常の状態では、プロタミン４は、シラノール基３と結合したままの状態を継続し、プロタミン４による抗菌作用が継続的に奏される。   6B, when protamine 4 is applied to the target surface 1, as shown in FIG. 6C, the protamine 4 is bonded to the silanol group 4, and the silica particle 2 is surrounded by the protamine 4. Let After that, even when a member having the target surface 1 is put in the oral cavity or the like, in a normal state, the protamine 4 continues to be bonded to the silanol group 3, and the antibacterial action by the protamine 4 continues. Played.

次に、長期使用により、対象表面１を有する部材のメンテナンスが必要となる場合のように、対象表面１を再処理すべき事態となったならば、対象表面１を有する部材を口腔から取り出し、酸性溶液５に漬ける。そのようにすると、図６（ｄ）に示す状態となる。酸性溶液５としては、上述したリン酸溶液のように強酸のものが好ましく用いられる。   Next, if it becomes a situation that the target surface 1 should be reprocessed, such as when maintenance of the member having the target surface 1 is required due to long-term use, the member having the target surface 1 is taken out from the oral cavity, Immerse in acidic solution 5. If it does so, it will be in the state shown in Drawing 6 (d). As the acidic solution 5, a strong acid like the phosphoric acid solution described above is preferably used.

酸性環境では、塩基性ペプチドが溶解し除去されることになるから、古いプロタミン４はシラノール基３と結合しない状態となり、図６（ｅ）に示すように、洗い流される。   In an acidic environment, since the basic peptide is dissolved and removed, the old protamine 4 is not bonded to the silanol group 3, and is washed away as shown in FIG. 6 (e).

その後、図６（ｆ）に示すように、対象表面１を中性又はアルカリ性の状態として、再度プロタミン４を塗布すれば、新しいプロタミン４がシラノール基３と結合し、抗菌作用を再度活性化することができる。   Thereafter, as shown in FIG. 6 (f), when the target surface 1 is in a neutral or alkaline state and the protamine 4 is applied again, the new protamine 4 binds to the silanol group 3 and activates the antibacterial action again. be able to.

本発明者が、ＪＩＳ Ｚ２８０１に定義された抗菌性試験を実施したところ、本願発明による抗菌処理方法は、少なくとも大腸菌、ブドウ球菌及びカンジタ菌に対して有効であることが確認された。また、本願発明の抗菌処理方法は、歯科領域に限らず、広く一般の医療領域にも同様に適用できる。例えば、手術あるいは処置により、体内に存置させる部材等（骨盤に打ち込まれて支点として利用される部材、義足の固定具等）にも適用できる。従来、このような部材は、体内に存知させると、感染症の温床となる危険性があったが、本願発明の抗菌処理方法を適用すると、かかる懸念を除去することができる。   When the inventor conducted an antibacterial test defined in JIS Z2801, it was confirmed that the antibacterial treatment method according to the present invention is effective at least against Escherichia coli, staphylococci and candida. In addition, the antibacterial treatment method of the present invention is not limited to the dental field and can be applied to a wide general medical field in the same manner. For example, the present invention can be applied to a member or the like that is left in the body by surgery or treatment (a member that is driven into the pelvis and used as a fulcrum, a prosthetic leg fixing device, or the like). Conventionally, when such a member is known in the body, there is a risk of becoming a hotbed for infectious diseases. However, when the antibacterial treatment method of the present invention is applied, such a concern can be eliminated.

本発明の一実施の形態における対象表面の拡大写真（無加工状態）Magnified photograph of target surface in one embodiment of the present invention (unprocessed state) 本発明の一実施の形態における対象表面の拡大写真（シリカ粒子蒸着加工後）Magnified photograph of target surface in one embodiment of the present invention (after silica particle deposition processing) 本発明の一実施の形態における対象表面の拡大写真（プロタミン付加後）Magnified photograph of target surface in one embodiment of the present invention (after adding protamine) 本発明の一実施の形態における対象表面の拡大写真（プロタミン除去後）Magnified photograph of target surface in one embodiment of the present invention (after removal of protamine) 本発明の一実施の形態における対象表面の拡大写真（プロタミン再付加後）Magnified photograph of target surface in one embodiment of the present invention (after re-addition of protamine) （ａ）〜（ｆ）本発明の一実施の形態における対象表面の処理方法の各工程を示す模式図(A)-(f) The schematic diagram which shows each process of the processing method of the target surface in one embodiment of this invention.

１ 対象表面
２ シリカ粒子
３ シラノール基
４ プロタミン
５ 酸性溶液 1 target surface 2 silica particles 3 silanol groups 4 protamine 5 acidic solution

Claims (5)

セラミックスを含み得る対象表面に無機粒子を結合させる工程と、
前記無機粒子に、抗菌性を有するタンパク又は前記タンパクから誘導される塩基性ペプチドを結合させる工程と、
前記対象表面を酸性環境とし、前記タンパク又は前記塩基性ペプチドを前記無機粒子から除去する工程とを含み、
前記無機粒子は、シラノール基が露出するシリカ粒子であり、
前記タンパクは、プロタミン、サルミン及びクルペインのいずれかを含む対象表面の抗菌処理方法。 Binding inorganic particles to a target surface that may contain ceramics ;
A step of binding an antibacterial protein or a basic peptide derived from the protein to the inorganic particles;
The target surface and acidic environment, look including the step of removing the protein, or the basic peptide from the inorganic particles,
The inorganic particles are silica particles in which silanol groups are exposed,
The method for antibacterial treatment of a target surface , wherein the protein includes any one of protamine, salmine, and curpain . 前記タンパク又は前記塩基性ペプチドを除去した前記対象表面を中性又はアルカリ性環境とし、前記タンパク又は前記塩基性ペプチドを前記無機粒子に結合させる工程とをさらに含む請求項１記載の対象表面の抗菌処理方法。   The antibacterial treatment of the target surface according to claim 1, further comprising the step of setting the target surface from which the protein or the basic peptide has been removed to a neutral or alkaline environment and binding the protein or the basic peptide to the inorganic particles. Method. 前記タンパクは、魚類の白子由来のタンパクである請求項１又は２に記載の対象表面の抗菌処理方法。 The method for antibacterial treatment of a target surface according to claim 1 or 2 , wherein the protein is a protein derived from a fish larva. 前記無機粒子を結合させる工程は、蒸着加工により実施される請求項１又は２記載の対象表面の抗菌処理方法。   The antibacterial treatment method for a target surface according to claim 1 or 2, wherein the step of bonding the inorganic particles is performed by vapor deposition. 前記無機粒子を結合させる工程は、サンドブラスト法により実施される請求項１又は２記載の対象表面の処理方法。   The method for treating a target surface according to claim 1 or 2, wherein the step of bonding the inorganic particles is performed by a sandblast method.
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