JP5976185B1 - Lignocellulosic biomass-derived compound production apparatus and production method - Google Patents

Abstract

An apparatus and a method for producing a lignocellulosic biomass-derived compound having high saccharification reaction efficiency while suppressing the amount of enzyme used are provided. The present invention relates to a first saccharification device containing pretreated lignocellulosic biomass and an enzyme, and a first saccharification solution produced by the first saccharification device and the first saccharification adsorbed by the enzyme. A second saccharification apparatus containing a residue, and a mechanism for maintaining a solid content concentration in the second saccharification apparatus at a higher concentration than a solid content concentration in the first saccharification apparatus. It is a manufacturing apparatus of a lignocellulosic biomass origin compound. [Selection figure] None

Description

  The present invention relates to an apparatus and a method for producing a lignocellulosic biomass-derived compound.

  In recent years, from the viewpoint of global warming countermeasures and effective utilization of waste, the use of biomass using plant resources as a raw material has attracted attention. In general, sugars such as sugar cane and starches such as corn are often used as raw materials for producing a compound such as ethanol from biomass. However, these raw materials are originally used as food or feed, and long-term utilization as industrial resources may cause competition with food or feed use and may cause a rise in raw material prices.

  Therefore, technology development that uses non-edible biomass as an energy resource is being promoted. Non-edible biomass includes the most abundant cellulose on the earth, most of which exists as lignocellulose, which is a complex with the aromatic polymer lignin and hemicellulose.

  As a method for producing a target compound from lignocellulosic biomass, first, pretreatment such as hydrothermal treatment is performed. At this time, if necessary, an acid or an alkali may be mixed as appropriate. Enzyme and water are added to the pretreated lignocellulosic biomass, and cellulose and hemicellulose in the biomass are hydrolyzed (saccharified) into monosaccharides of glucose and xylose, respectively, to obtain a sugar solution.

  The enzymes used in the saccharification reaction are cellulase and hemicellulase. Cellulase is a general term for several enzyme groups, and is generally marketed in a state where β-glucosidase (BGL), endoglucanase (EG), and cellobiohydrolase (CBH) are mixed. Pretreated lignocellulosic biomass consists of hemicellulose, cellulose and lignin with high polymerization degree, and celloose solubilized in the liquid because of the low degree of polymerization consisting of two monosaccharides, monosaccharide xylose, glucose, etc. It is included. The saccharification target of EG and CBH is solid cellulose, and the saccharification target of BGL is cellobiose solubilized in the liquid.

  Moreover, in the saccharification reaction, a phenomenon in which an enzyme adsorbs nonspecifically to lignin often occurs. EG and CBH have a cellulose binding module (CBM) and specifically adsorb also to cellulose as a substrate, but BGL does not have CBM, and therefore only non-specific adsorption to lignin. Most of the enzyme is adsorbed on the saccharification residue. Conventionally, the enzyme adsorbed on the saccharification residue is hardly released, and it is considered difficult to recover the enzyme from the supernatant after the reaction and reuse it (see Non-Patent Document 1).

  Even when BGL is adsorbed to lignin, the active site remains, and the saccharification ability is maintained for cellobiose in the liquid.

Patent Document 1 discloses a method of charging a saccharification residue to which an enzyme is attached from the beginning of the saccharification step in order to reuse the enzyme.
In Patent Document 2, a saccharification enzyme in a saccharified solution is adsorbed and recovered on lignocellulose, separated into an enzyme separated into a supernatant by coarse filtration and an enzyme adsorbed on lignocellulose. A method of reusing in the saccharification step is disclosed.

JP 2010-98951 A Japanese Patent No. 5431499

Yoshinori Kobayashi “A Comprehensive Analysis of Biomass Saccharification Reaction” Biotechnology Vol. 91, No. 10, pp. 556-560 (January 25, 2014)

In Patent Document 1, BGL can be reused, but the lignin concentration in the saccharification tank increases, EG and CBH are adsorbed, and the saccharification reaction efficiency of cellulose, which is a solid component, decreases.
In Patent Document 2, BGL can be reused as in Patent Document 1, but the lignin concentration in the saccharification tank increases, EG and CBH are adsorbed, and the saccharification reaction efficiency of cellulose, which is a solid component, decreases.

  This invention is made | formed in view of the said situation, Comprising: It aims at providing the manufacturing apparatus and manufacturing method of a lignocellulosic biomass origin compound with high saccharification reaction efficiency, suppressing an enzyme usage-amount.

That is, the present invention is as follows.
(1) A first saccharification device containing pretreated lignocellulosic biomass and an enzyme, a first saccharified solution produced by the first saccharification device, and lignin adsorbed with β-glucosidase (BGL) A second saccharification device containing the first saccharification residue, and a mechanism for maintaining the solid content concentration in the second saccharification device at a higher concentration than the solid content concentration in the first saccharification device. An apparatus for producing a lignocellulosic biomass-derived compound.
(2) The apparatus for producing a lignocellulosic biomass-derived compound according to (1), wherein the first saccharified solution contains cellooligosaccharide, and the second saccharification device contains glucose generated from the cellooligosaccharide by the BGL. .
( 3 ) The lignocellulosic biomass-derived compound according to (1) or (2) , wherein the mechanism for maintaining the high concentration is a mechanism for adjusting the discharge amount of the second saccharification residue from the second saccharification device. manufacturing device.
( 4 ) Furthermore, the fermenter containing the 2nd saccharification liquid produced | generated by said 2nd saccharification apparatus, the 2nd saccharification residue which the enzyme adsorb | sucked, and microorganisms, and the fermentation liquid produced | generated with the said fermentor, A mechanism for separating the fermentation residue adsorbed with the enzyme, and a pipe for feeding the separated fermentation residue from the solid-liquid separation device to the second saccharification device, and maintaining the high concentration The apparatus for producing a lignocellulosic biomass-derived compound according to (1) or (2) , which is a pipe that is introduced into the solid-liquid separation device and the second saccharification device.
( 5 ) An enzyme production tank for supplying an enzyme liquid to the first saccharification apparatus and an enzyme production tank containing pretreated lignocellulosic biomass, and the enzyme liquid and enzyme produced in the enzyme production tank A solid-liquid separation device for separating the enzyme production residue adsorbed by the liquid, a pipe for feeding the separated enzyme liquid from the solid-liquid separation device to the first saccharification device, and the separated enzyme production residue for the solid solution. The apparatus for producing a lignocellulosic biomass-derived compound according to any one of (1) to ( 4 ), comprising: a pipe that is supplied from a liquid separation device to the second saccharification device.
( 6 ) In any one of (1) to ( 5 ), the solid content concentration in the second saccharification device is 1.1 times or more and 6 times or less than the solid content concentration in the first saccharification device. The manufacturing apparatus of the lignocellulosic biomass origin compound of description.
(7) pre-Symbol second saccharification residue, any one of which said enzyme is adsorbed in at least one selected from the fermentation residue and the enzyme production residue is β- glucosidase (BGL) (1) ~ (6) An apparatus for producing a lignocellulosic biomass-derived compound as described in 1 above.

( 8 ) A first saccharification step in which pretreated lignocellulosic biomass and an enzyme are mixed and saccharified; a saccharified solution produced in the first saccharification step; and lignin on which β-glucosidase (BGL) is adsorbed A second saccharification step in which the first saccharification residue is mixed and saccharified, and the solid content concentration in the second saccharification step is adjusted to be higher than the solid content concentration in the first saccharification step a solid concentration adjusting step, was perforated, lignocellulose which the solid concentration adjustment step, characterized in that a step of adjusting the discharge amount of the second saccharification residue generated by the second saccharification step Of producing a biomass-derived compound.
(9) A first saccharification step in which pretreated lignocellulosic biomass and an enzyme are mixed and saccharified; a saccharified solution produced in the first saccharification step; and lignin adsorbed with β-glucosidase (BGL) A second saccharification step in which the first saccharification residue is mixed and saccharified, and the solid content concentration in the second saccharification step is adjusted to be higher than the solid content concentration in the first saccharification step The solid content concentration adjustment step, the second saccharification solution produced in the second saccharification step, the second saccharification residue adsorbed with the enzyme, and the fermentation step for mixing and fermenting the yeast, and the production in the fermentation step A solid-liquid separation step for separating the fermented fermentation broth and the fermentation residue adsorbed with the enzyme, wherein the solid content concentration adjusting step is configured to determine the fermentation residue adsorbed by the enzyme separated in the solid-liquid separation step. Put into the second saccharification equipment Method for producing a lignocellulosic biomass derived compounds, which is a degree.
(10) A first saccharification step in which pretreated lignocellulosic biomass and an enzyme are mixed and saccharified; a saccharified solution produced in the first saccharification step; and lignin to which β-glucosidase (BGL) is adsorbed A second saccharification step in which the first saccharification residue is mixed and saccharified, and the solid content concentration in the second saccharification step is adjusted to be higher than the solid content concentration in the first saccharification step A solid content concentration adjusting step, an enzyme producing step of mixing enzyme producing bacteria and pretreated lignocellulosic biomass to produce an enzyme, an enzyme solution produced in the enzyme producing step, and an enzyme production residue adsorbed with the enzyme A solid-liquid separation step, and a step of feeding the enzyme solution separated in the solid-liquid separation step into the first saccharification device , wherein the solid content concentration adjusting step is the solid-liquid separation step In minutes Method for producing a lignocellulosic biomass derived compounds enzyme is characterized in that the step of introducing the enzyme production residue adsorbed to the second saccharification device.
(11) In the second saccharification step, glucose is generated mainly from β-glucosidase (BGL) from the cellooligosaccharides generated in the first saccharification step. (8) to (10) The manufacturing method of the lignocellulosic biomass origin compound of description.
( 12 ) The solid content concentration in the second saccharification step is 1.1 times or more and 6 times or less than the solid content concentration in the first saccharification apparatus, according to any one of ( 8 ) to ( 11 ). Of producing a lignocellulosic biomass-derived compound.
(13) before Symbol second saccharification residue, any one of which said enzyme is adsorbed in at least one selected from the fermentation residue and the enzyme production residue is β- glucosidase (BGL) (8) ~ (12) The manufacturing method of the lignocellulosic biomass origin compound as described in 2.

  According to the apparatus and method for producing a lignocellulosic biomass-derived compound of the present invention, it is not necessary to immobilize an enzyme, and the amount of enzyme input can be reduced to efficiently carry out a saccharification reaction.

It is a figure which shows schematic structure of the manufacturing apparatus of the lignocellulosic biomass origin compound which concerns on 1st embodiment of this invention. It is a figure which shows schematic structure of the manufacturing apparatus of the lignocellulose biomass origin compound which concerns on 2nd embodiment of this invention. It is a figure which shows schematic structure of the manufacturing apparatus of the lignocellulose biomass origin compound which concerns on 3rd embodiment of this invention. It is a figure which shows schematic structure of the manufacturing apparatus of the lignocellulose biomass origin compound which concerns on 4th embodiment of this invention. It is a figure which shows schematic structure of the manufacturing apparatus of the lignocellulosic biomass origin compound which concerns on 5th embodiment of this invention. It is a figure which shows schematic structure of the manufacturing apparatus of the lignocellulosic biomass origin compound which concerns on 6th embodiment of this invention. In Experiment 1, it is the graph which plotted the time-dependent change of the cellobiose density | concentration with respect to the cellobiose density | concentration of the early stage of saccharification reaction of the sample from which the addition amount of a BGL adsorption residue differs. 2 is a graph plotting changes in glucose concentration over time in Example 1 and Comparative Example 1. FIG.

  The lignocellulosic biomass to be treated by the production apparatus and production method of the present invention mainly contains cellulose, hemicellulose, and lignin. , Rice husk, wheat straw, wood chips, wood fiber, chemical pulp, waste paper, plywood and other agricultural and forestry resources, sugarcane bagasse, sugarcane foliage, corn stover, etc. Plant tissue. These lignocellulosic biomasses may be used alone or as a mixture.

In the present invention, hemicellulose is a so-called pentose containing 5 carbons such as xylose and a so-called hexose containing 6 carbons such as mannose, arabinose and galacturonic acid. Furthermore, since it has complex polysaccharides such as glucomannan and glucuronoxylan, when it undergoes hydrolysis, it will be a pentose monosaccharide consisting of five carbons and a pentose oligosaccharide in which multiple monosaccharides are linked. , 6 carbon hexose monosaccharides, hexose saccharide oligosaccharides linked to multiple monosaccharides, pentose saccharide monosaccharides and hexose saccharide monosaccharides linked together Is produced.
Cellulose has 6 carbons as a structural unit, so when hydrolyzed, it produces 6-carbon monosaccharides composed of 6 carbons and hexose oligosaccharides in which a plurality of monosaccharides are linked. In general, the composition ratio and production amount of monosaccharides and / or oligosaccharides vary depending on the pretreatment method and the types of agricultural and forestry product resources, agricultural and forestry product wastes and processed agricultural and forestry products used as raw materials.

  Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. In each of the drawings, portions not related to the description may be omitted.

<First embodiment>
FIG. 1 is a diagram showing a schematic configuration of an apparatus for producing a lignocellulosic biomass-derived compound according to the first embodiment of the present invention.
The production apparatus 10 of the present embodiment includes a first saccharification apparatus 1 including pretreated lignocellulosic biomass and an enzyme, and a first saccharification liquid and an enzyme generated by the first saccharification apparatus 1 that are adsorbed to the first saccharification apparatus 1. The second saccharification device 2 containing the saccharification residue is disposed via a pipe. Furthermore, the second saccharified solution produced by the second saccharification device 2 serves as a mechanism for maintaining the solid content concentration in the second saccharification device 2 higher than the solid content concentration in the first saccharification device 1. And the second saccharification residue adsorbed by the enzyme, and the second saccharification residue adsorbed by the enzyme separated in the solid-liquid separation device 4 is returned to the second saccharification solution. A return pipe 5 is provided.
In FIG. 1, as an example, the fermenter 3 is disposed adjacent to the second saccharification device 2, but the equipment following the second saccharification device 2 should be appropriately selected according to the use of the saccharification solution. Can do.

  The first saccharification device 1 is a device for mixing a pretreated lignocellulosic biomass and an enzyme to perform a saccharification reaction, and there is no particular limitation. Examples thereof include a stirring type, aeration stirring type, a bubble column type, a fluidized bed type, and a packed bed type.

  The pretreated lignocellulosic biomass means lignocellulosic biomass that has been pretreated in order to efficiently perform a saccharification reaction. Examples of the pretreatment method include a steaming method using only steam, a method using an ionic liquid, and a pulverizing method using a mill. Further, in the pretreatment, an acid or an alkali may be appropriately mixed as necessary. The acid is selected from sulfuric acid, hydrochloric acid, nitric acid, phosphoric acid and the like, and these may be used alone or in combination. Among them, sulfuric acid which is inexpensive and easily available is particularly preferable for industrial use. The alkali is selected from sodium hydroxide, potassium hydroxide, and ammonia, and these may be used alone or in combination. Although there is no particular limitation on the reaction vessel used for the hydrothermal treatment reaction, the heat pressure vessel having acid resistance or alkali resistance, or the form in which the vessel having acid resistance or alkali resistance is placed in a heating pressure device such as an autoclave is processed. Can be considered.

The enzyme in the present invention means an enzyme that decomposes lignocellulosic biomass into monosaccharides or oligosaccharide units, and any enzyme that decomposes lignocellulosic biomass into monosaccharides or oligosaccharides can be used. Cellulase and hemicellulase As long as it has each activity of.
Cellulase may be any one that decomposes cellulose into monosaccharides or oligosaccharides such as glucose, and has at least one activity of endoglucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (BGL). It is preferable from a viewpoint of enzyme activity that it is an enzyme mixture which has each of these activity.
Similarly, hemicellulase may be any one that decomposes hemicellulose into monosaccharides or oligosaccharides such as xylose, and has at least one activity of each activity of xylanase, xylosidase, mannanase, pectinase, galactosidase, glucuronidase, and arabinofuranosidase. From the viewpoint of enzyme activity, an enzyme mixture having each of these activities is preferable.
In the present invention, the “enzyme active ingredient” means each of these saccharifying enzymes when an enzyme mixture is used, and means the saccharifying enzyme used when a single saccharifying enzyme is used.
The origin of these cellulases and hemicellulases is not limited, and cellulases and hemicellulases such as filamentous fungi, basidiomycetes, and bacteria can be used.

  The second saccharification device 2 mixes the first saccharification solution produced in the first saccharification device 1 and the first saccharification residue adsorbed with the enzyme, and the solid content concentration in the first saccharification device 1 is more than that. It is a tank for performing a saccharification reaction at a high solid content concentration, and there is no particular limitation. For example, an aeration stirring type, a bubble column type, a fluidized bed type, a packed bed type and the like can be mentioned. In the first saccharified solution, glucose, xylose, cellooligosaccharide and the like produced by the pretreatment reaction and the first saccharification apparatus are solubilized. Cellooligosaccharide means an oligosaccharide composed of 2 to 6 monosaccharides such as cellobiose and serves as a substrate for BGL. The first saccharification residue contains solid components such as unreacted cellulose, hemicellulose, and lignin, and the enzyme is adsorbed on these solid components.

  In the present specification, "mechanism for maintaining a high concentration" means a mechanism for keeping the solid content concentration in the second saccharification device 2 higher than the solid content concentration in the first saccharification device 1, For example, a mechanism for adjusting the input amount of the pretreated lignocellulosic biomass to the first saccharification device 1, a mechanism for adjusting the discharge amount of the first saccharification residue fed from the first saccharification device 1, The mechanism etc. which adjust the discharge | emission amount of the 2nd saccharification residue from the 2nd saccharification apparatus 2 are mentioned. Among these, a mechanism for adjusting the discharge amount of the residue from the second saccharification device 2 is preferable. More specifically, the second saccharification from the second saccharification device 2 to the fermentor 3 as shown in the solid-liquid separation device 4 as shown in FIG. 1 and the embodiments described later (see FIGS. 2 to 5). Examples include a pipe for feeding only the liquid or only the second saccharification residue, a filter, a weir, a fermentation liquid produced in the fermentation tank 3, and a solid-liquid separation device 13 for separating the fermentation residue adsorbed with the enzyme. It is done.

  The solid-liquid separation device 4 is a device for separating the second saccharified solution produced in the second saccharification device 2 and the second saccharification residue adsorbed with the enzyme, and is not particularly limited. A device capable of adjusting the separation strength is preferred. For example, a device that can adjust the separation strength by adjusting the applied pressure, a device that can adjust the separation strength by changing the mesh size of the sieve mesh or filter, and a solid-liquid separation device Examples include an apparatus that can lower the separation strength by discharging a part of the solid after the solid-liquid separation without changing the operating conditions. More specifically, a filter press, a screw press, and a vibrating sieve having a mesh of 100 to 2360 μm can be mentioned. This is because, when the saccharification apparatus is an aeration and stirring type, if the solid content concentration is high, there is a fear that the power used increases. Therefore, first, when the production apparatus is started up, the separation strength of the solid-liquid separation apparatus 4 is increased and the second saccharification residue is accumulated in the second saccharification apparatus 2. Then, after the start-up, the solid content concentration in the second saccharification device 2 is reduced by lowering the separation strength of the solid-liquid separation device 4 and discharging part of the second saccharification liquid and second saccharification residue. Can be maintained at a concentration higher than the solid concentration in the first saccharification apparatus 1.

  The return pipe 5 is used for feeding the second saccharification residue adsorbed by the enzyme separated in the solid-liquid separation device 4 to the second saccharification device 2, and is not particularly limited.

Next, an example of the manufacturing method using the manufacturing apparatus 10 of the lignocellulosic biomass origin compound shown in FIG. 1 is demonstrated.
First, in a steamer, lignocellulosic biomass is mixed with acid or alkali and hydrothermally treated to obtain pretreated lignocellulosic biomass. At this time, only the steaming step may be performed, or after the steaming step in a pressurized and humidified state, an explosion step of pulverizing lignocellulosic biomass may be performed by a method such as releasing the pressure at once. Next, the pretreated lignocellulosic biomass and the enzyme are introduced into the first saccharification apparatus 1 with a pump or the like through a pipe to perform a saccharification reaction. At this time, the temperature of the first saccharification apparatus 1 is preferably 45 ° C to 70 ° C. The first saccharification reaction time is preferably 12 hours to 120 hours.
The first saccharification solution produced in the first saccharification step and the first saccharification residue adsorbed with the enzyme are input to the second saccharification device 2. At this time, the temperature of the second saccharification device 2 is preferably 45 ° C to 70 ° C. The second saccharification reaction time is preferably 12 hours to 120 hours.
Furthermore, the second saccharification liquid produced in the second saccharification step and the second saccharification residue adsorbed with the enzyme are introduced into the solid-liquid separation device 4 through a pipe, and the second saccharification liquid and the second saccharification residue are introduced. And to separate.

  The separated saccharified solution is sent to the fermenter 3. On the other hand, the separated second saccharification residue is introduced into the second saccharification device 2 through the return pipe 5 and circulated, so that the solid content concentration in the second saccharification device 2 is reduced to the first saccharification device 1. It can be kept at a higher concentration than the solid content concentration. In addition, the solid content concentration in the second saccharification device 2 is adjusted, as described above, by adjusting the separation strength in the solid-liquid separation device 4, thereby adjusting the discharge amount of the second saccharification residue. The solid content concentration in 2 can be adjusted.

  In the present specification, the solid content concentration adjusting step means a step of adjusting the solid content concentration in the second saccharification step so as to be kept higher than the solid concentration in the first saccharification step. As a method of adjusting the solid content concentration, for example, a method of adjusting the input amount of pretreated lignocellulosic biomass into the first saccharification device 1 or a first solution fed from the first saccharification device 1 is used. Examples thereof include a method of adjusting the discharge amount of the saccharification residue, a method of adjusting the discharge amount of the second saccharification residue from the second saccharification device 2, and the like. Among these, a method for adjusting the discharge amount of the residue from the second saccharification device 2 is preferable. More specifically, the method using the solid-liquid separation device 4 as shown in FIG. 1 and the second saccharification device 2 to the fermenter 3 as shown in the embodiments described later (see FIGS. 2 to 5). Method using pipe for feeding only second saccharified solution or only second saccharified residue, method using filter 8, method using weir 12, fermented solution and enzyme generated in fermenter 3 The method using the solid-liquid separation apparatus 13 for isolate | separating the fermentation residue which adsorb | sucked is mentioned.

As described above, the enzyme includes cellulase and hemicellulase, and cellulase is an enzyme that produces monosaccharide or oligosaccharide using cellulose as a substrate, and includes endoglucanase (EG), cellobiohydrolase (CBH), and β-glucosidase. (BGL) mixture. EG acts randomly on the cellulose chain to produce cellooligosaccharide, and CBH acts from the end of the cellulose chain to produce cellobiose. Furthermore, BGL produces | generates glucose which is a monosaccharide from the cellooligosaccharide produced | generated by EG and CBH.
BGL is strongly hydrophobic and has the property of being easily adsorbed to lignin having a strong hydrophobicity contained in lignocellulosic biomass. However, the adsorption site of BGL on lignin is often other than the active site, and glucose can be produced by contacting cellooligosaccharides such as cellobiose in the liquid even when adsorbed on lignin.
Conventionally, after the saccharification step, a saccharification residue containing lignin adsorbed with BGL is discharged. However, according to the saccharifying enzyme utilization method of the present invention, the first saccharified solution produced in the first saccharification step, the first saccharification residue containing lignin adsorbed with BGL, and the BGL adsorbed by the enzyme circulation step are adsorbed. Saccharification of BGL and cellooligosaccharide by providing a second saccharification device containing a second saccharification residue and having a solid concentration higher than that of the first saccharification device and performing a second saccharification step Perform the reaction efficiently.
Therefore, in this embodiment, among the enzymes adsorbed on the first saccharification residue and the second saccharification residue, β-glucosidase (BGL) is utilized to efficiently perform the saccharification reaction in the second saccharification device. Can be done.

  Therefore, in the first saccharification step, a saccharification reaction for generating cellooligosaccharide from cellulose by EG and CBH and a saccharification reaction for generating glucose from cellooligosaccharide by BGL are performed. Since cellulose is a solid content, in order to efficiently contact EG and CBH with cellulose, the solid content concentration in the first saccharification apparatus 1 needs to be kept at a low concentration. The following is preferable, and 10% to 15% is more preferable. Next, in the second saccharification step, a saccharification reaction for generating glucose from the cellooligosaccharides produced in the first saccharification step is performed mainly by BGL. As described above, since BGL can be contacted with cellooligosaccharide solubilized in the first saccharification solution while adsorbed on the first saccharification residue, the saccharification reaction can be performed in the second saccharification device 2. The solid content concentration can be made higher than the solid content concentration in the first saccharification apparatus 1, and is preferably 1.1 to 6 times the solid content concentration in the first saccharification apparatus. 2 times or more and 5 times or less are more preferable.

In this specification, the “solid content concentration” means that a certain weight of the solid-liquid mixture (A) is in an absolutely dry state (the material is dried at a temperature of 100 to 110 ° C. until a constant mass is reached, and is evaporated inside the material. It means the ratio of the solid content weight (B) that remains when it is dried until no water is present, and is represented by the following formula [1].
(Solid content concentration) [%] = (B) / (A) × 100 [1]

  About the process after a saccharification process, it can select suitably by the utilization method of a saccharified liquid. For example, when it aims at obtaining ethanol, a fermentation process can be performed using a saccharified liquid, and a distillation process can be provided as a purification process subsequently.

<Second embodiment>
FIG. 2 is a diagram showing a schematic configuration of the apparatus for producing a lignocellulose-based biomass-derived compound according to the second embodiment of the present invention. In the production apparatus 20 of the present embodiment, the first saccharification apparatus 1 and the second saccharification apparatus 2 are arranged via a pipe, and further, the pipe 6 is provided above the second saccharification apparatus 2. In the lower part, a pipe 7 is arranged toward the fermenter 3. The manufacturing apparatus 20 of the present embodiment is not provided with the solid-liquid separation device 4 and the pipe 5 but is provided with the pipe 6 and the pipe 7 in that the manufacturing apparatus 10 shown in FIG. The other configurations are the same as those of the manufacturing apparatus 10.
In FIG. 2, the same components as those shown in FIG. 1 are denoted by the same reference numerals. Moreover, in FIG. 2, the fermenter 3 is arrange | positioned adjacent to the saccharification apparatus 2 as an example, However, The subsequent installation can be suitably selected according to the use of saccharified liquid.

  The pipe 6 is a mechanism for adjusting the discharge amount of the second saccharification residue from the second saccharification device 2, and the second saccharification device 2 generates the second saccharification residue generated by precipitation. The saccharified solution is a pipe for introducing the saccharified solution into the fermenter 3, and is not particularly limited.

  The pipe 7 is a mechanism for adjusting the discharge amount of the second saccharification residue from the second saccharification device 2, and the second saccharification device 2 generates the second saccharification residue generated by precipitation. The saccharification residue is a pipe for introducing the saccharification residue into the fermenter 3, and is not particularly limited.

Next, an example of the manufacturing method using the manufacturing apparatus 20 of the lignocellulosic biomass origin compound shown in FIG. 2 is demonstrated.
The process is the same as in the first embodiment until the first saccharification process and the second saccharification process are performed. After the second saccharification step, the second saccharification liquid and the second saccharification residue are separated in the second saccharification device 2 by leaving for a while. The standing time depends on the solid content concentration contained in the second saccharification apparatus 2, but is preferably 24 hours to 120 hours. After standing, the second saccharified solution is put into the fermenter 3 through the pipe 6. At the start-up of the production apparatus, only the second saccharified solution is introduced from the pipe 6 into the fermenter. However, after the start-up, the first saccharification residue from the first saccharification apparatus 1 accumulates, The solid content concentration in the second saccharification device 2 is adjusted to the first saccharification by adjusting the amount of the second saccharification residue introduced into the fermentation tank 3 from the pipe 7 with respect to the amount charged from the saccharification device 1. The concentration can be kept higher than the solid content concentration in the apparatus 1.
In this embodiment, since it has the mechanism which adjusts the discharge | emission amount of the 2nd saccharification residue from the 2nd saccharification apparatus 2 inside the 2nd saccharification apparatus 2, the point which does not require a special installation is excellent. .
About the process after a saccharification process, it can select suitably with the utilization method of a saccharified liquid similarly to 1st embodiment.

<Third embodiment>
FIG. 3 is a diagram showing a schematic configuration of the apparatus for producing a lignocellulosic biomass-derived compound according to the third embodiment of the present invention. In the production apparatus 30 of the present embodiment, the first saccharification apparatus 1 and the second saccharification apparatus 2 are disposed via a pipe. Further, a filter 8 is disposed in the second saccharification device 2, and a pipe 9 is located closer to the first saccharification device 1 than the filter 8 of the second saccharification device 2, and the fermenter 3 is located more than the filter 8. A pipe 11 is disposed toward the fermenter 3 at a close position.
The manufacturing apparatus 30 of the present embodiment is not shown in FIG. 2 in that the pipe 6 and the pipe 7 are not provided, and the filter 8, the pipe 9, and the pipe 11 are provided. Unlike the apparatus 20, the other configurations are the same as those of the manufacturing apparatus 20.
In FIG. 3, the same reference numerals are used for the same components as those shown in FIG. Moreover, in FIG. 3, the fermenter 3 is arrange | positioned adjacent to the saccharification apparatus 2 as an example, However, The subsequent installation can be suitably selected according to the use of saccharified liquid.

  The filter 8 is a mechanism for adjusting the discharge amount of the second saccharification residue from the second saccharification device 2, and holds the second saccharification residue adsorbed by the enzyme in the second saccharification device 2 so as not to be discharged. Although there is no particular limitation, the apparatus is preferably 10 μm or more, and more preferably 100 μm or more, because it may cause clogging if the mesh of the filter 8 is too fine. Moreover, since the residue cannot be hold | maintained and is discharged | emitted when the fineness of a mesh is too rough, 1 cm or less is preferable and 5 mm or less is more preferable.

  The pipe 9 is a pipe for introducing a part of the second saccharification residue into the fermenter 3 and is not particularly limited.

  The pipe 11 is a pipe for introducing the second saccharified solution generated in the second saccharification apparatus 2 and separated by the filter 8 into the fermenter 3, and there is no particular limitation.

Next, an example of the manufacturing method using the manufacturing apparatus 30 of the lignocellulosic biomass origin compound shown in FIG. 3 is demonstrated.
The process up to the point where the first saccharification process and the second saccharification process are performed is the same as in the second embodiment. Of the second saccharification solution produced in the second saccharification step and the second saccharification residue adsorbed with the enzyme, the second saccharification solution is separated by the filter 8 and is put into the fermenter 5 via the pipe 11. Is done. On the other hand, the second saccharification residue is accumulated in the second saccharification device 2 by the filter 8 when the production apparatus is started up. However, since the first saccharification residue 1 is input from the first saccharification device 1 as needed, the second saccharification residue is added to the fermenter after the start-up with respect to the amount input from the first saccharification device 1. By adjusting the amount charged into 3, the solid content concentration in the second saccharification device 2 can be kept higher than the solid content concentration in the first saccharification device 1.
In this embodiment, since it has the mechanism which adjusts the discharge | emission amount of the 2nd saccharification residue from the 2nd saccharification apparatus 2 inside the 2nd saccharification apparatus 2, the point which does not require a special installation is excellent. .
About the process after a saccharification process, it can select suitably with the utilization method of a saccharified liquid similarly to 1st embodiment.

<Fourth embodiment>
FIG. 4 is a diagram showing a schematic configuration of a lignocellulosic biomass-derived compound producing apparatus according to a fourth embodiment of the present invention. In the production apparatus 40 of the present embodiment, the first saccharification device 1 and the second saccharification device 2 are arranged via a pipe. Furthermore, a weir 12 is disposed in the second saccharification device 2, and a pipe 9 is located closer to the first saccharification device 1 than the weir 12 of the second saccharification device 2. A pipe 11 is disposed toward the fermenter 3 at a close position.
The manufacturing apparatus 40 of this embodiment is different from the manufacturing apparatus 30 shown in FIG. 3 in that the filter 8 is not provided and the weir 12 is provided, and the other configurations are the same as the manufacturing apparatus 30. It is.
In FIG. 4, the same components as those shown in FIG. 3 are denoted by the same reference numerals. Moreover, in FIG. 4, the fermenter 3 is arrange | positioned adjacent to the saccharification apparatus 2 as an example, However, The subsequent installation can be suitably selected according to the use of saccharified liquid.

  The weir 12 is a mechanism for adjusting the discharge amount of the second saccharification residue from the second saccharification device 2 and is held so that the second saccharification residue adsorbed by the enzyme in the second saccharification device 2 is not discharged. There is no special limitation.

Next, an example of the manufacturing method using the manufacturing apparatus 40 of the lignocellulose biomass origin compound shown in FIG. 4 is demonstrated.
The process up to the first saccharification process and the second saccharification process is the same as in the third embodiment. Of the second saccharification liquid produced in the second saccharification step and the second saccharification residue adsorbed with the enzyme, the second saccharification liquid is separated by the weir 12 and is put into the fermenter 5 via the pipe 11. Is done. On the other hand, the second saccharification residue is accumulated in the second saccharification device 2 by the weir 12 when the production apparatus is started up. However, since the first saccharification residue 1 is input from the first saccharification device 1 as needed, the second saccharification residue is added to the fermenter after the start-up with respect to the amount input from the first saccharification device 1. By adjusting the amount charged into 3, the solid content concentration in the second saccharification device 2 can be kept higher than the solid content concentration in the first saccharification device 1.
In this embodiment, since it has the mechanism which adjusts the discharge | emission amount of the 2nd saccharification residue from the 2nd saccharification apparatus 2 inside the 2nd saccharification apparatus 2, the point which does not require a special installation is excellent. .
About the process after a saccharification process, it can select suitably with the utilization method of a saccharified liquid similarly to 1st embodiment.

<Fifth embodiment>
FIG. 5 is a diagram showing a schematic configuration of a lignocellulosic biomass-derived compound producing apparatus according to a fifth embodiment of the present invention. In the production apparatus 50 of the present embodiment, the first saccharification device 1 and the second saccharification device 2 are arranged via a pipe. Furthermore, the fermenter 3 containing the 2nd saccharification liquid and the 2nd saccharification residue which the enzyme produced | generated by the 2nd saccharification apparatus 2 adsorb | sucked via the piping 6 ', and microorganisms is arrange | positioned. Moreover, the solid-liquid separator 13 which isolate | separates the fermentation residue produced | generated by the fermenter 3 and the fermentation residue which the enzyme adsorb | sucked, and piping which throws the isolate | separated fermentation residue into the 2nd saccharification apparatus 2 from the solid-liquid separator 13 14 are arranged.
The manufacturing apparatus 50 of the present embodiment is different from the manufacturing apparatus 20 shown in FIG. 2 in that a pipe 6 ′, a solid-liquid separator 13, and a pipe 14 are provided instead of the pipes 6 and 7. The other configurations are the same as those of the manufacturing apparatus 20.
In FIG. 5, the same components as those shown in FIG.

  In the present embodiment, the solid-liquid separation device 13 and the pipe 14 are used as a mechanism for maintaining the solid content concentration in the second saccharification device 2 at a higher concentration than the solid content concentration in the first saccharification device 1. be able to.

  The fermenter 3 is a tank for performing fermentation containing the second saccharified liquid separated in the solid-liquid separator 4, a part of the second saccharification residue, and microorganisms, and there is no particular limitation. . In the second saccharified solution, glucose, xylose, cellooligosaccharide and the like produced by the pretreatment reaction, the first saccharification device and the second saccharification device are solubilized. The second saccharification residue contains solid components such as cellulose, hemicellulose, and lignin, and the enzyme is adsorbed on these solid components.

  The microorganism used in the fermentation process is not particularly limited as long as it can produce the target lignocellulosic biomass-derived compound. Specific examples include yeasts and bacteria, and genetically modified microorganisms are also preferably used. A genetically modified microorganism refers to a microorganism that does not have an enzyme gene required for conversion to a target lignocellulosic biomass-derived compound, such as alcohol, by introducing these genes using genetic engineering technology. This enables generation of a cellulosic biomass-derived compound. Examples of the genetically modified microorganism include genetically modified Escherichia coli having alcohol fermentability.

  In the present invention, the lignocellulosic biomass-derived compound means a lignocellulosic biomass-derived compound produced by ingesting monosaccharides and oligosaccharides obtained by decomposing lignocellulosic biomass. For example, alcohols such as ethanol, butanol, 1,3-propanediol, 1,4-butanediol, glycerol, organic acids such as pyruvic acid, succinic acid, malic acid, itaconic acid, citric acid, lactic acid, inosine, guanosine, etc. Examples thereof include nucleotides such as nucleoside, inosinic acid and guanylic acid, and diamine compounds such as cadaverine. When the compound obtained by fermentation is a monomer such as lactic acid, it may be converted into a polymer by polymerization.

  The solid-liquid separation device 13 is a mechanism that adjusts the discharge amount of the second saccharification residue from the second saccharification device 2, and separates the fermentation residue generated in the fermenter 3 and the fermentation residue adsorbed by the enzyme. Although there is no particular limitation, it is preferable that the apparatus can adjust the solid-liquid separation strength in the same manner as the solid-liquid separation apparatus 4 described above.

  The pipe 14 is for feeding the fermentation residue adsorbed by the enzyme separated in the solid-liquid separator 13 to the second saccharification apparatus 2 and is not particularly limited.

Next, an example of the manufacturing method using the manufacturing apparatus 50 of the lignocellulosic biomass origin compound shown in FIG. 5 is demonstrated.
The process up to the point where the first saccharification process and the second saccharification process are performed is the same as in the second embodiment. The total amount of the second saccharified solution and the second saccharification residue generated in the second saccharification step is charged into the fermenter 3 and used in the fermentation step. At this time, the temperature of the fermenter 3 is preferably 25 ° C to 50 ° C. The fermentation time is preferably 24 hours to 120 hours. The fermentation liquid and fermentation residue produced | generated in the fermentation process are thrown into the solid-liquid separator 13 via piping, and are isolate | separated into a fermentation liquid and a fermentation residue. The separated fermentation broth contains the lignocellulosic biomass-derived compound and is purified in the subsequent steps. On the other hand, the separated fermentation residue is put into the second saccharification device 2 via the pipe 14 and reused in the second saccharification step. Further, as described above, the solid content concentration in the second saccharification device 2 is kept higher than the solid content concentration in the first saccharification device 1 by adjusting the separation strength in the solid-liquid separation device 13. be able to.

  In the present invention, the fermentation residue means a solid component remaining after the fermentation step, and includes solids such as cellulose, hemicellulose, and lignin, and enzymes adsorbed on these solids.

  In this embodiment, saccharification reaction can be efficiently performed in the second saccharification device 2 by utilizing β-glucosidase (BGL) among the enzymes adsorbed on the fermentation residue.

<Sixth embodiment>
FIG. 6 is a diagram showing a schematic configuration of a lignocellulosic biomass-derived compound producing apparatus according to a sixth embodiment of the present invention. In the manufacturing apparatus 60 of the present embodiment, the first saccharification apparatus 1, the second saccharification apparatus 2, and the fermenter 3 are arranged via a pipe, A pipe 6 is arranged at the lower part of the pipe 7 toward the fermenter 3. The fermenter 3 is provided with a solid-liquid separation device 13 and a pipe 14 for feeding the separated fermentation residue from the solid-liquid separation device 13 to the second saccharification device 2. Furthermore, the enzyme production tank 15 containing the enzyme producing bacteria for supplying the enzyme liquid to the first saccharification apparatus 1 and the pretreated lignocellulosic biomass, and the enzyme liquid and the enzyme produced in the enzyme production tank 15 are adsorbed. A solid-liquid separation device 16 for separating the enzyme production residue, a pipe 17 for feeding the separated enzyme liquid from the solid-liquid separation device 16 to the first saccharification device 1, and a solid-liquid separation device 16 for separating the separated enzyme production residue To the second saccharification device 2 is disposed.
The manufacturing apparatus 60 of the present embodiment is different from the manufacturing apparatus 50 shown in FIG. 5 in that the enzyme production tank 15, the solid-liquid separator 16, the pipe 17, and the pipe 18 are disposed. Other configurations are the same as those of the manufacturing apparatus 50.
In FIG. 6, the same components as those shown in FIG.
The structure of the mechanism for maintaining the solid content concentration in the second saccharification device 2 higher than the solid content concentration in the first saccharification device 1 is the first embodiment, the second embodiment, and the third embodiment. The form and those shown in the fourth embodiment may be used.
Moreover, in FIG. 6, the fermenter 3 is arrange | positioned adjacent to the saccharification apparatus 2 as an example, However, The subsequent installation can be suitably selected according to the use of saccharified liquid.

  The enzyme production tank 15 contains an enzyme-producing bacterium and pretreated lignocellulosic biomass, and is a tank for the enzyme producing bacterium to produce an enzyme using the pretreated lignocellulosic biomass as a substrate, and there is no particular limitation. The temperature of the enzyme production tank 15 is preferably 25 ° C to 55 ° C. The time is preferably 2 to 14 days.

  The enzyme-producing bacterium is a bacterium that produces a saccharification enzyme used in the saccharification reaction in the first saccharification device and the second saccharification device, and there is no particular limitation, and filamentous fungi, basidiomycetes, bacteria, etc. may be used. it can.

  The solid-liquid separation device 16 is a device for separating the enzyme solution generated in the enzyme production tank 15 and the enzyme production residue adsorbed with the enzyme, and is not particularly limited. Similarly, it is preferable that the apparatus can adjust the solid-liquid separation strength.

The piping 17 is for supplying the enzyme solution separated in the solid-liquid separation device 16 to the first saccharification device 1, and is not particularly limited.
The pipe 18 is for feeding the enzyme production residue adsorbed by the enzyme separated in the solid-liquid separation device 16 to the second saccharification device 2, and is not particularly limited.

Next, an example of the manufacturing method using the manufacturing apparatus 60 of the lignocellulosic biomass origin compound shown in FIG. 6 is demonstrated.
First, enzyme producing bacteria and pretreated lignocellulosic biomass are introduced into the enzyme production tank 15, and enzyme production is performed using the pretreated lignocellulose biomass as a substrate. At this time, the substrate is not particularly limited as long as it becomes a substrate for enzyme-producing bacteria, but the residue is reused in the second saccharification apparatus 2 by using pretreated lignocellulosic biomass as a substrate. be able to.

  The enzyme solution and enzyme production residue produced in the enzyme production process are put into the solid-liquid separation device 16 via a pipe and separated into the enzyme solution and the enzyme production residue. The separated enzyme solution is introduced into the first saccharification device 1 via the pipe 17 and used in the first saccharification step. On the other hand, the separated enzyme production residue is put into the second saccharification device 2 via the pipe 18 and reused in the second saccharification step. Further, the solid content concentration in the second saccharification device 2 is maintained at a higher concentration than the solid content concentration in the first saccharification device 1 by adjusting the separation strength in the solid-liquid separation device 16 as described above. be able to.

The enzyme solution means a solution containing an enzyme produced by an enzyme-producing bacterium, and includes cellulase and hemicellulase. As described above, CBH and EG in cellulase are mainly saccharified in the first saccharification apparatus 1. To be used.
The enzyme production residue means a solid component remaining after the enzyme production step, and includes solids such as cellulose, hemicellulose, and lignin, and enzymes adsorbed on these solids.
In this embodiment, saccharification reaction can be efficiently performed in the second saccharification device 2 by utilizing β-glucosidase (BGL) among the enzymes adsorbed on the enzyme production residue.

  Hereinafter, the present invention will be described in more detail with reference to specific examples. However, the present invention is not limited to the following examples.

[Test Example 1] Cellobiose solution saccharification test using BGL adsorption residue <Preparation of BGL adsorption residue>
Using 10 g-dry of pretreated lignocellulosic biomass (10 g-dry means 10 g in a dry state) as an enzyme substrate, it was diluted to 10 mass% in water. The enzyme derived from Trichoderma reesei was added to this 10% by mass substrate solution so as to be 15 FPU (Filter Paper Unit) / g-dryBM. 15 FPU / g-dryBM indicates an enzyme amount such that the filter paper decomposition activity amount is 15 with respect to 1 g of dry biomass. A saccharification reaction is carried out at 50 ° C. for 96 hours to obtain a large residue of particles by solid-liquid separation using a sieve, and a small residue of particles is obtained by solid-liquid separation by centrifugation at 3500 rpm for 20 minutes. It was. Each was washed with 50 mM citrate buffer, and solid-liquid separation was performed again by sieving or centrifugation at 3500 rpm for 20 minutes. A large residue of washed particles and a small residue of particles were combined to obtain a BGL adsorption residue. The solid content concentration in the BGL adsorption residue was 29%, and the water content was 71%.

<Cellobiose solution saccharification test>
An 8.5 mass% cellobiose solution was prepared and added to the 300 ml flask so that the solid content concentration of the BGL adsorption residue was 0%, 5%, 10%, and 15%. It added so that the total amount of an 8.5 mass% cellobiose solution and a BGL adsorption | suction residue might be set to 50 g, and the specific numerical value was shown in following Table 3.

  Each saccharification reaction was carried out for 144 hours while stirring and stirring. After 0 hours, 4 hours, 24 hours, 48 hours, 72 hours, and 144 hours, samples were taken at 1.0 g each, and subjected to high performance liquid chromatography (SHIMADZU, HPLC reducing sugar system) and Asahipak MH2p- Cellobiose concentration was measured using a 50 4E column (manufactured by shodex). The results are shown in FIG.

<Results and discussion>
The vertical axis in FIG. 7 represents the ratio of the cellobiose concentration over time to the initial (after 0 hour) cellobiose concentration, and the horizontal axis represents time. When the BGL adsorption residue is 0%, the BGL adsorption residue is 5%, 10%, and 15%, and the cellobiose concentration decreases with time. It was revealed that cellobiose was decomposed by BGL contained in the BGL adsorption residue and changed to glucose.

[Example 1]
Lignocellulosic biomass 10 g-dry was used as an enzyme substrate, diluted to 10% by mass in water, and 100 g of a substrate solution was prepared in a 300 ml flask. An enzyme derived from Trichoderma reesei was added to the substrate solution so as to be 4FPU / g-dryBM. The first saccharification reaction was performed by shaking and stirring at 50 ° C. for 24 hours. After 24 hours, 27.5 g of BGL adsorption residue (solid content concentration: 23%) prepared in the same manner as in Test Example 1 was added to the saccharification solution. A second saccharification reaction was performed. It added so that the solid content concentration of the BGL adsorption | suction residue in a saccharification solution might be 5%. 1.0 g samples were taken at 0 hours, 24 hours (second saccharification reaction start point), 48 hours and 72 hours after the start of the first saccharification reaction, and subjected to high performance liquid chromatography (manufactured by SHIMADZU, HPLC The glucose concentration was measured using a reducing sugar system) and an Asahipak MH2p-50 4E column (manufactured by shodex). The results are shown in FIG.

[Comparative Example 1]
The first saccharification reaction was performed in the same manner as in Example 1, and the saccharification reaction was further performed as it was without adding the BGL adsorption residue. 1.0 g samples were collected at 0, 24, 48 and 72 hours after the start of the first saccharification reaction, and subjected to high performance liquid chromatography (SHIMADZU, HPLC reducing sugar system) and Asahipak MH2p-50. The glucose concentration was measured using a 4E column (manufactured by shodex). The results are shown in FIG.

  FIG. 8 is a graph plotting changes in glucose concentration over time in Example 1 and Comparative Example 1. Although the plots are almost overlapped up to 24 hours after the addition of the BGL adsorption residue, the glucose concentration in Example 1 is higher than that in Comparative Example 1 after 24 hours. Therefore, it became clear that by adding the BGL adsorption residue, cellooligosaccharides such as cellobiose contained in the liquid were decomposed by BGL to produce glucose.

  From the above, in the apparatus for producing a lignocellulosic biomass-derived compound according to the present invention, it is not necessary to immobilize the enzyme by reusing the enzyme adsorbed on the residue, reducing the input amount of the enzyme, and improving efficiency. It was revealed that the saccharification reaction can be carried out.

  DESCRIPTION OF SYMBOLS 1 ... 1st saccharification apparatus, 2 ... 2nd saccharification apparatus, 3 ... Fermenter, 4, 13, 16 ... Solid-liquid separation apparatus, 5 ... Return pipe, 6, 6 ', 7, 9, 11, 14, 17, 18 ... piping, 8 ... filter, 10, 20, 30, 40, 50, 51, 60 ... lignocellulosic biomass-derived compound production apparatus, 12 ... weir, 15 ... enzyme production tank.

Claims (13)

A first saccharification device comprising pretreated lignocellulosic biomass and enzyme;
A second saccharification apparatus comprising a first saccharification residue comprising a first saccharification solution produced by the first saccharification apparatus and lignin adsorbed with β-glucosidase (BGL) ;
A mechanism for maintaining the solid content concentration in the second saccharification device at a higher concentration than the solid content concentration in the first saccharification device;
An apparatus for producing a lignocellulosic biomass-derived compound, comprising: The first saccharified solution contains cellooligosaccharide,
2. The apparatus for producing a lignocellulosic biomass-derived compound according to claim 1, wherein the second saccharification apparatus contains glucose generated from the cellooligosaccharide by the BGL. The apparatus for producing a lignocellulosic biomass-derived compound according to claim 1 or 2 , wherein the mechanism for maintaining the high concentration is a mechanism for adjusting the discharge amount of the second saccharification residue from the second saccharification apparatus. Furthermore, the second saccharification liquid produced in the second saccharification apparatus and the second saccharification residue adsorbed with the enzyme, and a fermenter containing microorganisms,
A solid-liquid separation device for separating the fermentation liquor produced in the fermenter and the fermentation residue adsorbed with the enzyme;
A pipe for feeding the separated fermentation residue from the solid-liquid separator to the second saccharification apparatus;
With
The apparatus for producing a lignocellulosic biomass-derived compound according to claim 1 or 2 , wherein the mechanism for maintaining the high concentration is a pipe that is introduced into the solid-liquid separation device and the second saccharification device. Furthermore, an enzyme production tank for supplying an enzyme solution to the first saccharification apparatus and an enzyme production tank containing pretreated lignocellulosic biomass,
A solid-liquid separation device for separating the enzyme solution produced in the enzyme production tank and the enzyme production residue adsorbed by the enzyme;
A pipe for feeding the separated enzyme solution from the solid-liquid separation device to the first saccharification device;
A pipe for feeding the separated enzyme production residue from the solid-liquid separation device to the second saccharification device;
The apparatus for producing a lignocellulose-based biomass-derived compound according to any one of claims 1 to 4 . The solid content concentration in the second saccharification device is 1.1 to 6 times the solid content concentration in the first saccharification device, and the lignocellulose system according to any one of claims 1 to 5. Production equipment for biomass-derived compounds. Before Stories second saccharification residue, the fermentation residue and lignocellulose according to any one of the enzymes produced according to claim 1-6 enzyme is β- glucosidase (BGL) at least adsorb one to be selected from the residue For producing biomass-derived compounds. A first saccharification step in which pretreated lignocellulosic biomass and enzyme are mixed and saccharified;
A second saccharification step of mixing and saccharifying a saccharification solution produced in the first saccharification step and a first saccharification residue containing lignin adsorbed with β-glucosidase (BGL) ;
A solid content concentration adjusting step for adjusting the solid content concentration in the second saccharification step so as to maintain a higher concentration than the solid content concentration in the first saccharification step;
I have a,
The method for producing a lignocellulosic biomass-derived compound, wherein the solid content concentration adjusting step is a step of adjusting the discharge amount of the second saccharification residue generated in the second saccharification step . A first saccharification step in which pretreated lignocellulosic biomass and enzyme are mixed and saccharified;
A second saccharification step of mixing and saccharifying a saccharification solution produced in the first saccharification step and a first saccharification residue containing lignin adsorbed with β-glucosidase (BGL);
A solid content concentration adjusting step for adjusting the solid content concentration in the second saccharification step so as to maintain a higher concentration than the solid content concentration in the first saccharification step;
A fermentation process in which the second saccharified solution produced in the second saccharification process and the second saccharification residue adsorbed with the enzyme, and yeast are mixed and fermented;
A solid-liquid separation step of separating the fermentation liquor produced in the fermentation step and the fermentation residue adsorbed with the enzyme,
The method for producing a lignocellulosic biomass-derived compound, wherein the solid content concentration adjusting step is a step of feeding a fermentation residue adsorbed by the enzyme separated in the solid-liquid separation step into a second saccharification apparatus. A first saccharification step in which pretreated lignocellulosic biomass and enzyme are mixed and saccharified;
A second saccharification step of mixing and saccharifying a saccharification solution produced in the first saccharification step and a first saccharification residue containing lignin adsorbed with β-glucosidase (BGL);
A solid content concentration adjusting step for adjusting the solid content concentration in the second saccharification step so as to maintain a higher concentration than the solid content concentration in the first saccharification step;
An enzyme production process for producing enzymes by mixing enzyme-producing bacteria and pretreated lignocellulosic biomass;
A solid-liquid separation step for separating the enzyme solution produced in the enzyme production step and the enzyme production residue adsorbed with the enzyme;
Introducing the enzyme solution separated in the solid-liquid separation step into the first saccharification device;
Have
The method for producing a lignocellulosic biomass-derived compound, wherein the solid content concentration adjusting step is a step of feeding an enzyme production residue adsorbed by the enzyme separated in the solid-liquid separation step into a second saccharification device . The lignocellulosic system according to any one of claims 8 to 10, wherein glucose is produced from the cellooligosaccharide produced in the first saccharification step mainly by β-glucosidase (BGL) in the second saccharification step. A method for producing a biomass-derived compound. The lignocellulosic biomass according to any one of claims 8 to 11 , wherein the solid content concentration in the second saccharification step is 1.1 to 6 times the solid content concentration in the first saccharification apparatus. A method for producing a derived compound. Before Stories second saccharification residue, lignocellulose according to any one of the fermentation residue and the enzyme production residue enzyme adsorbed to at least one selected from the claims 8-12 is β- glucosidase (BGL) Of producing a biomass-derived compound.

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