JP5967527B2 - Appetite increase and weight gain inhibitor - Google Patents

Description

  The present invention relates to an appetite enhancing and weight gain inhibitor.

  Lactobacillus brevis (Lactobacillus brevis) SBC8803 strain (Accession number: FERM BP-10632) has an antiallergic action (Patent Document 1), an alcoholic liver disorder suppressing action (Patent Document 2), and an intestinal tract protecting action. (Patent Document 3) and the like are known.

International Publication No. 2008/023663 International Publication No. 2009/090961 JP 2010-83881 A

  An object of the present invention is to provide a novel use of the Lactobacillus brevis SBC8803 strain.

  The present inventors have found that feeding mice with the SBC8803 strain or treated product thereof increases appetite and suppresses the increase in body weight despite an increase in food intake. .

  That is, the present invention provides an appetite-enhancing and weight gain-inhibiting agent containing the SBC8803 strain belonging to Lactobacillus brevis or a processed product thereof as an active ingredient.

  According to the above-mentioned appetite increase and weight gain inhibitor, an extremely surprising effect of suppressing an increase in body weight is achieved in spite of an increase in appetite. Therefore, for example, when you do not want to increase weight but want to increase appetite (for example, increase appetite when there is no appetite, summer appetite, increase appetite for the elderly, etc.) It is.

  In addition, the Lactobacillus brevis SBC8803 strain was established on June 28, 2006 at the National Institute of Advanced Industrial Science and Technology, Patent Biological Deposit Center (1st, 1st East, 1st Street, Tsukuba City, Ibaraki Prefecture, Japan, 6th postal code 305-8586). ), And the accession number is FERM BP-10632. In the present specification, this strain is also referred to as “SBL88 strain”.

  Lactobacillus brevis is a type of lactic acid bacterium that has been used for fermented foods for a long time, and has been established to be safe for living organisms. Therefore, the appetite promoting and weight gain suppressing agent of the present invention is highly safe to the living body and can be taken continuously for a long period of time. Therefore, it can be used as a pharmaceutical ingredient, a food / beverage product component, a food / beverage product additive, and the like.

  ADVANTAGE OF THE INVENTION According to this invention, the safety improvement with respect to a biological body and the novel appetite increase and weight gain inhibitor which can be used also as a component of food-drinks are provided. Moreover, the pharmaceutical, food / beverage products, food / beverage product additive containing the said appetite increase and weight gain inhibitor are provided.

It is a graph which shows the food intake amount transition of the mouse | mouth in an Example. It is a graph which shows the weight transition of the mouse | mouth in an Example.

  Hereinafter, preferred embodiments of the present invention will be described in detail.

  The appetite promoting and weight gain inhibitor of the present invention contains, as an active ingredient, the bacterial body of SBC8803 strain belonging to Lactobacillus brevis or a processed product thereof.

  For the appetite promoting and body weight gain inhibitor of the present invention, the above bacterial cells and the processed product thereof may be used singly or in combination of two or more.

  The microbial cells may be live cells or dead cells. Bacteria can be produced in large quantities by culturing viable bacteria. The medium may be either a liquid medium or a solid medium, but preferably contains a nitrogen source and a carbon source. Nitrogen sources include meat extract, peptone, gluten, casein, yeast extract, amino acids, etc., and carbon sources include glucose, xylose, fructose, inositol, maltose, water candy, soup, starch, bacus, bran, Molasses, glycerin and the like can be used. Further, ammonium sulfate, potassium phosphate, magnesium chloride, sodium chloride, iron, manganese, molybdenum and the like can be added as inorganic substances, and vitamins and the like can be further added. Suitable media include MRS media, LBS media, Rogosa media, WYP media, GYP media and the like.

  The above strain can be cultured according to a culture method commonly used for culturing strains belonging to Lactobacillus brevis. For example, the culture temperature is usually 20-50 ° C, preferably 25-40 ° C, more preferably 30 ° C. The culture time is usually 6 to 62 hours, preferably 12 to 48 hours, and more preferably 15 to 30 hours. The pH of the medium is usually 3 to 8, preferably 4 to 7, and more preferably 6 to 7. Cultivation may be performed in an incubator, or aeration and shaking may be performed during the cultivation.

  Examples of treated cells include treated products obtained by subjecting the above-mentioned cells (live cells or dead cells) to treatment such as heating, pressurization, drying, crushing, crushing, breaking, or self-dissolution. . Two or more of these treatments may be combined. As the treated product of the microbial cells, for example, a treated product obtained by heating the microbial cells at 100 ° C. or more for several minutes or more (for example, by subjecting the microbial cells to autoclaving at a temperature of 105 to 125 ° C. for 10 minutes or more. Processed product), processed product obtained by freeze-drying, spray-drying, etc. on the bacterial cells, processed product obtained by contacting the bacterial cells with an organic solvent (acetone, ethanol, etc.), A treated product obtained by contacting with an alkaline solution, a treated product obtained by enzymatic disruption of bacterial cells, or a treated product obtained by physically destroying the bacterial cells with ultrasonic waves, a French press, or the like can be used. Such a cell-treated product is preferable in that it is easy to handle as compared with untreated cells (particularly live cells).

  The appetite enhancing and weight gain inhibitor of the present invention may be in any form such as a solid (for example, a powder obtained by freeze-drying), a liquid (a water-soluble or fat-soluble solution or suspension), a paste, or the like. In addition, any dosage form such as powder, pill, granule, tablet, syrup, troche, capsule and the like may be used.

  The above-mentioned various preparations may consist only of the above-mentioned fungus bodies or processed products thereof, which are active ingredients, and can be prepared, for example, by molding the fungus bodies or treated products thereof into the above dosage form. . The above-mentioned various preparations also contain the above active ingredients and pharmaceutically acceptable additives (excipients, binders, lubricants, disintegrants, emulsifiers, surfactants, bases, solubilizers, suspensions). It is also possible to prepare the mixture by mixing with an agent and the like. In this case, the content of the active ingredient is, for example, 0.5 to 50% by mass based on the total amount of the preparation.

  For example, the excipient includes lactose, sucrose, starch, dextrin and the like. Examples of the binder include polyvinyl alcohol, gum arabic, tragacanth, gelatin, hydroxypropylmethylcellulose, hydroxypropylcellulose, sodium carboxymethylcellulose, polyvinylpyrrolidone and the like. Examples of the lubricant include magnesium stearate, calcium stearate, talc and the like. Examples of the disintegrant include crystalline cellulose, agar, gelatin, calcium carbonate, sodium bicarbonate, dextrin and the like. Examples of the emulsifier or surfactant include Tween 60, Tween 80, Span 80, and glyceryl monostearate. Examples of the base include cetostearyl alcohol, lanolin, polyethylene glycol, rice bran oil, fish oil (DHA, EPA, etc.), olive oil and the like. Examples of the solubilizer include polyethylene glycol, propylene glycol, sodium carbonate, sodium citrate, Tween 80 and the like. Examples of the suspending agent include Tween 60, Tween 80, Span 80, glyceryl monostearate, polyvinyl alcohol, polyvinyl pyrrolidone, methyl cellulose, hydroxymethyl cellulose, sodium alginate and the like.

  The appetite promoting and weight gain inhibitor of the present invention may be administered to humans or non-human mammals. The dosage and administration method can be appropriately determined according to the condition, age, etc. of the individual to be administered. Suitable administration methods include, for example, oral administration. As an example of the dosage and administration method, there can be mentioned a method in which an appetite enhancing and weight gain inhibitor is orally administered in an amount of 0.5 to 500 mg of an active ingredient once a day.

  The appetite promoting and weight gain inhibitor of the present invention can be used as a pharmaceutical ingredient, a food / beverage product ingredient, a food / beverage food additive, a feed ingredient, a feed additive, and the like.

  For example, the appetite promoting and weight gain inhibitor of the present invention is water, soft drinks, fruit juice drinks, milk drinks, alcoholic drinks, breads, noodles, rice, tofu, dairy products, fermented foods, fermented milk, soy sauce, It can be used as an additive to food and drink such as miso and confectionery. These foods and drinks may further contain other additives usually used in the art, and examples of such additives include bitters, flavors, apple fibers, soybean fibers, meat extracts, blacks. Vinegar extract, gelatin, corn starch, honey, animal and vegetable oils; monosaccharides such as glucose and fructose; disaccharides such as sucrose; polysaccharides such as dextrose and starch; sugar alcohols such as erythritol, xylitol, sorbitol, and mannitol; vitamin C and the like Vitamins. The appetite-enhancing and weight gain inhibitor of the present invention can also be used as a component for foods for specified health use, foods for special uses, dietary supplements, health foods, functional foods, foods for the sick, and the like. A food and drink containing the appetite enhancing and weight gain inhibitor of the present invention is a fermented product obtained by fermenting milk, skim milk, soy milk, vegetables, fruit juice, cereals, processed products thereof, and the like with the above strains. Also good.

  Hereinafter, the present invention will be described more specifically based on examples and the like. However, the present invention is not limited to the following examples.

[Example 1]
(Test method)
<Preparation of treated cells>
SBL88 strain was inoculated into a medium (composition: maltose 2 mass%, yeast extract 1.4 mass%, sodium acetate 0.5 mass%, manganese sulfate 0.005 mass%, pH 6.5-7.0), 30 The culture was allowed to stand at 1 ° C. for 1 day. The obtained culture solution (about 8 × 10 ⁸ cfu / ml) was centrifuged at 8,000 rpm for 10 minutes, and the cells were collected. The collected cells were resuspended in distilled water and centrifuged at 8,000 rpm for 10 minutes to collect the cells. This operation was repeated twice. The collected cells were suspended in distilled water, heat-treated at 105 ° C. for 10 minutes, and then freeze-dried to obtain heat-treated cell powder (cell-treated product).

<Preparation of mouse feed>
After adding 0.5% by mass of the cell-treated product of SBL88 strain to powdered feed CE-2 (manufactured by Clea Japan Co., Ltd.), pelleted mouse feed containing the cell-treated product (CE-2 feed containing SBL88) Prepared. As a control, a powdered feed CE-2 was pelleted to prepare a mouse feed (CE-2 feed) that does not contain a processed bacterial cell product.

<Mouse breeding>
C3H / HeN strain mice (3 weeks old male, Japan SLC Co., Ltd.) were bred for 2 weeks. After acclimatization, the mice were divided into 2 groups (5 animals for each group), CE-2 diet was given to the control group, and SBL88-containing CE-2 diet was given to the test group (SBL88 group) for 4 weeks. Ad libitum.

<Measurement of food intake and body weight>
Food intake and mouse body weight were measured every week during the free feeding period. The amount of food intake (g / day) was determined by measuring the amount of decrease in food per week and converting it to the amount of decrease per day. The food intake and body weight of each group were determined as an average value of 5 mice in each group. The presence or absence of a significant difference was determined using Student's t test.

(result)
FIG. 1 is a graph showing changes in the amount of food consumed by mice. Two weeks after the start of the test (2 weeks of breeding period), the amount of food consumed in the SBL88 group significantly increased at a risk rate of 5% compared to the control group. This tendency becomes more prominent 3 weeks after the start of the test (3 weeks of breeding) and 4 weeks after (4 weeks of breeding), both of which are significantly higher in the SBL88 group compared to the control group at a risk rate of 5%. Increased.

  FIG. 2 is a graph showing the weight transition of the mouse. The weights of the control group and the SBL88 group were the same (control group: 21.33 ± 0.45 g, SBL88 group: 21.11 ± 0.36 g) immediately after the start of the test (bred period 0 weeks). Surprisingly, from 2 weeks after the start of the test when the amount of food in the SBL88 group was increased compared to the control group (2 weeks of breeding period) to 4 weeks (4 weeks of breeding period), the body weight of the SBL88 group was There was no difference in body weight of the group (no statistically significant difference), but rather there was a tendency to suppress weight gain compared to the control group.

  The mechanism by which weight gain is suppressed while the appetite in the SBL88 group is promoted is not clear, but the reason for this is, for example, that the metabolism is activated by ingesting the treated product of SBL88 strain, It is conceivable that the physical strength is improved and the amount of activity (consumption) is activated by the promotion.

  According to the appetite enhancing and weight gain inhibitor of the present invention, an extremely surprising effect of suppressing an increase in body weight is achieved in spite of an increase in appetite. Therefore, for example, when you don't want to increase your weight but want to increase your appetite (for example, when you have no appetite due to summer batter, etc.) Or it can utilize for the food-drinks, pharmaceuticals, etc. for taking.

Claims (4)

  An appetite-enhancing and weight gain inhibitor containing, as an active ingredient, a cell of SBC8803 (accession number: FERM BP-10632) belonging to Lactobacillus brevis or a processed product thereof. A pharmaceutical product for increasing appetite and suppressing weight gain, comprising, as an active ingredient, a cell of SBC8803 (accession number: FERM BP-10632) belonging to Lactobacillus brevis or a processed product thereof . A food and drink for promoting appetite and suppressing weight gain, comprising, as an active ingredient, a cell of SBC8803 (accession number: FERM BP-10632) belonging to Lactobacillus brevis or a processed product thereof . A food additive for increasing appetite and suppressing body weight gain, comprising as an active ingredient a cell of SBC8803 (accession number: FERM BP-10632) belonging to Lactobacillus brevis or a processed product thereof.

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