JP5852539B2 - Centrifuge container - Google Patents

Description

  The present invention relates to a centrifuge container for centrifuging a component of a specimen in a container by being rotated about a central axis of the container.

  Conventionally, devices such as Patent Documents 1 and 2 have been known as centrifuges for centrifuging a sample component such as blood.

  This centrifuge device stores a specimen such as blood inside a centrifuge container, and centrifuges the components of the specimen in the container by rotating the centrifuge container around the central axis as a rotation axis. .

  The centrifuge container used in the above-described centrifuge includes a reservoir that stores the specimen inside the container body, and an opening for injecting the specimen into the reservoir is provided in the container body. It is inclined so as to become higher from the center toward the outer periphery, and has an annular trap portion having a volume for storing a centrifugally separated component having a high specific gravity.

  In the trap part, a separation gel having an intermediate specific gravity between components to be separated in the specimen is arranged, and if the container is rotated while the specimen is injected into the storage part, only the component having a higher specific gravity than the separation gel is provided. Moves to the outer peripheral side of the separation gel in the trap portion, and the component having a specific gravity lower than that of the separation gel remains inside the separation gel, whereby the components of the specimen can be separated.

US Patent Application Publication No. 2007/0077183 International Publication No. 2008/140742

  By the way, when injecting the sample into the centrifuge container as described above, the sample is injected into the storage part from the opening provided in the container using a pipette, a syringe or the like. The specimen may adhere to the surface.

  In such a state, if the centrifuge container is put into the centrifuge and rotated as it is, the sample attached to the outside of the centrifuge container is scattered by the centrifugal force inside the centrifuge, The inside of the device may become contaminated.

  For example, when blood is used as a specimen, the inside of the centrifuge device may be contaminated with blood, and if a user or service person touches the contaminated portion, there is a risk of infection. Until now, measures have been taken to prevent the user from touching the container housing part of the centrifuge so as not to touch the contaminated part, or by providing the container housing part inside the apparatus. In such a case, since it is necessary to open and close the shutter and move the centrifuge container to the inside of the apparatus, the configuration of the centrifuge apparatus becomes complicated, or after the centrifuge container is inserted into the centrifuge apparatus It takes a long time until the container is taken out after the end of the centrifugation, leading to a decrease in measurement efficiency. Thus, contamination by the specimen causes various problems.

  The present invention has been made in view of the above problems, and in a centrifuge container for centrifuging a component of a specimen in a container by being rotated about the central axis of the container, an opening outside the container is provided. An object of the present invention is to provide a centrifuge container capable of preventing the centrifuge from being contaminated even when the centrifuge is performed in a state where a specimen is attached in the vicinity.

  The centrifuge container of the present invention includes a reservoir for storing the specimen inside the container body, and an opening for injecting the specimen into the reservoir is provided in the container body, and the container body rotates about the central axis of the container body. A centrifuge container for centrifuging the components of the specimen in the reservoir, wherein the specimen attached to the vicinity of the opening is radially outward centered about the central axis rather than the opening. It is characterized by having a scattering prevention wall for preventing scattering outside the container due to centrifugal force.

  In the centrifugal separation container of the present invention, the scattering prevention wall can be a cylindrical shape concentric with the central axis.

  Further, the anti-scattering wall includes an anti-scattering portion extending inward, such as a protrusion on an annular ring provided on a cylindrical inner peripheral surface or an inclined wall in which the cylindrical wall is inclined inward. Preferably.

  Further, it is preferable to provide a recess such as a groove extending in the circumferential direction on the inner peripheral surface of the scattering prevention wall.

  According to the centrifuge container of the present invention, the scattering that prevents the sample attached to the vicinity of the opening from splashing outside the container due to the centrifugal force to the container main body, radially outward with the central axis as the center. Even if the centrifuge container is inserted into the centrifuge and rotated with the specimen attached near the opening outside the container, the specimen scattered by the centrifugal force is not scattered by the scattering prevention wall. Since the sample is received, the sample is not scattered outside the container, and the centrifugal separator can be prevented from being contaminated with the sample.

  In the centrifuge container of the present invention, if the anti-scattering wall has a cylindrical shape concentric with the central axis, it is possible to effectively prevent the specimen from scattering.

  In this case, if the anti-scattering wall is provided with an anti-scattering part extending inward, the anti-scattering part further moves the specimen from the vicinity of the opening by centrifugal force to reach the anti-scattering wall. Since movement can be suppressed, scattering of the specimen outside the container can be more reliably suppressed as compared with an embodiment having only an inner wall surface extending in parallel with the central axis.

  In addition, when a dent is provided on the inner peripheral side of the scattering prevention wall, the specimen that has moved outward from the vicinity of the opening by centrifugal force and reached the scattering prevention wall accumulates in the dent, and further movement is suppressed. Therefore, scattering of the specimen out of the container can be more reliably suppressed.

The top view of the container for centrifugation of one embodiment of the present invention Sectional view of the centrifuge container (sectional view taken along line II-II in FIG. 1) Schematic configuration diagram of a centrifuge using the above centrifuge container The figure which shows the state at the time of the sample injection | throwing-in in the said container for centrifugation. The figure which shows the state at the time of centrifugation in the said container for centrifugation The figure which shows the state after centrifugation in the said container for centrifugation Sectional drawing (1) which shows the other aspect of the container for centrifugation of this invention. Sectional drawing (2) which shows the other aspect of the container for centrifugation of this invention.

  Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. FIG. 1 is a top view of a centrifuge container according to an embodiment of the present invention, and FIG. 2 is a cross-sectional view of the centrifuge container (a cross-sectional view taken along the line II-II in FIG. 1).

  The centrifuge container 1 according to the present embodiment includes a storage unit 11 that stores a specimen inside a container body 10 that is substantially cylindrical in appearance, and injects the specimen into the storage unit 11 on the upper surface of the container body 10. The opening 12 is provided.

  The container body 10 is formed by fitting and fixing the upper member 10a and the lower member 10b, and the lower surface of the storage portion 11 formed between the upper member 10a and the lower member 10b is an outer periphery from the center. An annular trap portion 13 having a volume for storing a high specific gravity component centrifuged from the specimen is formed on the outer peripheral portion of the storage portion 11. In the trap portion 13, a separation gel 14 having an intermediate specific gravity between components to be separated in the specimen is disposed. For example, when blood is used as a specimen and blood is separated into a blood cell component and a plasma component, a gel having a specific gravity intermediate between the blood cell component and the plasma component may be used as the separation gel 14.

  Further, a scattering prevention wall 20 is provided on the upper surface side of the container body 10 to prevent the specimen attached near the opening 12 from scattering due to centrifugal force. The scattering prevention wall 20 has a cylindrical shape concentric with the central axis C of the container body 10 and is provided so that its upper end is positioned higher than the opening 12 and is integrally formed with the upper member 10a. ing.

  Next, a centrifuge using the centrifuge container will be described. FIG. 3 is a schematic configuration diagram of a centrifuge using the centrifuge container.

  The centrifuge 50 includes an accommodating portion 52 that accommodates the centrifuge container 1 inside the housing 51. The accommodating portion 52 is provided with a holding portion 53 for holding the centrifuge container 1, and this holding portion 53 is rotatably supported by a rotation mechanism (not shown), and the centrifuge held by the holding portion 53. The center axis C of the separation container 1 is configured to coincide with the rotation axis of the rotation mechanism.

  Next, operational effects when the specimen in the centrifuge container is centrifuged in the centrifuge will be described. FIG. 4 is a diagram showing a state of the centrifuge container when the sample is charged, FIG. 5 is a diagram showing a centrifuge state of the centrifuge container, and FIG. 6 is a centrifuge state of the centrifuge container. FIG. Here, a case where blood is used as a sample is shown as an example.

  As shown in FIG. 4, blood 30 is first injected into the reservoir 11 of the centrifuge container 1. Specifically, blood 30 is injected from the opening 12 provided on the upper surface of the container body 10 by a pipette, a syringe, or the like. At this time, the blood 30 adheres to the vicinity of the opening 12 outside the container body 10. There is.

  Next, the centrifuge container 1 into which the blood 30 has been injected is accommodated in the accommodating portion 52 of the centrifuge 50 and rotated about the central axis C of the centrifuge container 1 as a rotation axis. Then, as shown in FIG. 5, only the blood cell component 31 having a specific gravity higher than that of the separation gel 14 moves to the outer peripheral side of the separation gel 14 in the trap portion 13, and the plasma component having a specific gravity lower than that of the separation gel 14. 32 sticks to the inner peripheral side of the separation gel 14.

  At this time, if the blood 30 is attached in the vicinity of the opening 12 on the outside of the container body 10, the blood 30 scatters outward in the radial direction around the central axis C due to centrifugal force. Although the container 52 of the centrifuge 50 is contaminated with the blood 30, the centrifuge container 1 according to the present embodiment is provided with the scattering prevention wall 20 around the opening 12, and the scattered blood. Since 30 is received by the scattering prevention wall 20, it is possible to prevent the accommodating portion 52 from being contaminated by the blood 30.

  Then, when the rotation of the centrifuge container 1 is stopped, as shown in FIG. 6, the blood cell component 31 on the outer peripheral side of the separation gel 14 remains trapped in the trap part 13 and is more internal than the separation gel 14. Only the plasma component 32 on the circumferential side descends and is stored near the center of the lower surface of the storage unit 11. Thereby, only the plasma component 32 can be extracted.

  Thus, if the centrifuge container 1 that can prevent the centrifuge device 50 from being contaminated is used, the centrifuge container housing portion 52 of the centrifuge device 50 will not be contaminated. Strict measures that make it difficult for users and service personnel to touch are unnecessary, leading to simplification of the structure of the centrifugal separator 50. Furthermore, if the structure of the centrifuge 50 is simplified, the time from when the centrifuge container 1 is put into the centrifuge 50 to when the centrifuge is finished and the container is taken out can be shortened. As a result, it becomes possible to perform a quick measurement.

  As mentioned above, although preferable embodiment of this invention was described, this invention is not limited to the said embodiment.

  For example, the scattering prevention wall may be provided with a scattering suppression portion that extends inward (in the direction toward the central axis).

  Specifically, as shown in FIG. 7, the inner wall surface of the anti-scattering wall 21 may be formed to be inclined inward. In this case, the whole inclined part functions as a scattering suppression part.

  Further, as shown in FIG. 8, a scattering suppression portion 22 a extending inward may be formed in a part of the scattering prevention wall 22 whose inner wall surface extends in parallel with the central axis C. In addition, the scattering suppression part 22a is not restricted to the aspect formed in the upper end part of the scattering prevention wall 22, You may form in any part of the inner peripheral side of the scattering prevention wall 22 if it is above the opening 12. FIG.

  When the anti-scattering wall is provided with the anti-scattering part extending inward (direction toward the central axis) as described above, it is difficult to form integrally with the upper member 10a of the container body 10 by injection molding or the like. Therefore, the upper member 10a and the anti-scattering wall may be formed separately, and both may be fixed.

  With such an embodiment, it is possible to more reliably suppress the scattering of the specimen outside the container as compared with the case where the scattering prevention wall has only the inner wall surface extending in parallel with the central axis.

  Moreover, as shown in FIG. 7, even when the dent 25 is provided on the inner peripheral side of the scattering prevention wall, scattering of the specimen can be further suppressed. In addition, about this dent 25, you may arrange | position many dot-shaped dents in the shape of a dimple along an inner periphery other than making it an annular dent along an inner periphery. Even in such an embodiment, the scattering of the specimen to the outside of the container can be more reliably suppressed as compared with the case where the scattering prevention wall has only the inner wall surface extending in parallel with the central axis.

  Further, the scattering prevention wall may be provided with both of the above-described scattering suppressing portion and the dent, and if it is in such a mode, scattering of the specimen outside the container can be more reliably suppressed.

  In addition to the above, it goes without saying that various improvements and modifications may be made without departing from the scope of the present invention.

DESCRIPTION OF SYMBOLS 1 Centrifugal container 10 Container main body 10a Upper member 10b Lower member 11 Reservoir part 12 Opening 13 Trap part 14 Separation gel 20 Anti-scattering wall 21 Anti-scattering wall 22 Anti-scattering wall 22a Anti-scattering part 30 Blood 31 Blood cell component 32 Plasma Component 50 Centrifugal separator 51 Housing 52 Housing part 53 Holding part

Claims (2)

The container main body is provided with a reservoir for storing the specimen, and an opening for injecting the specimen into the reservoir is provided in the container main body, and the container main body is rotated about the central axis of the container main body as described above. A centrifuge container for centrifuging a sample component in a reservoir,
The container main body is concentric with the central axis to prevent the specimen attached near the opening from being scattered radially outside the opening from the container due to centrifugal force . It is provided with a scattering prevention wall provided in a cylindrical shape so that its upper end is located higher than the opening,
A centrifuge container, wherein a recess is provided on the inner peripheral side of the scattering prevention wall . The scattering prevention wall, container for centrifugation according to claim 1, further comprising a scattering reduction unit extending inwardly.

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