JP5836791B2 - Beverage containing orotic acid and method for producing the same - Google Patents
Beverage containing orotic acid and method for producing the same Download PDFInfo
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- JP5836791B2 JP5836791B2 JP2011283548A JP2011283548A JP5836791B2 JP 5836791 B2 JP5836791 B2 JP 5836791B2 JP 2011283548 A JP2011283548 A JP 2011283548A JP 2011283548 A JP2011283548 A JP 2011283548A JP 5836791 B2 JP5836791 B2 JP 5836791B2
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- orotic acid
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- potassium
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- PXQPEWDEAKTCGB-UHFFFAOYSA-N orotic acid Chemical compound OC(=O)C1=CC(=O)NC(=O)N1 PXQPEWDEAKTCGB-UHFFFAOYSA-N 0.000 title claims description 168
- 229960005010 orotic acid Drugs 0.000 title claims description 83
- 235000013361 beverage Nutrition 0.000 title claims description 55
- 238000004519 manufacturing process Methods 0.000 title claims description 13
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 32
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 31
- 239000011734 sodium Substances 0.000 claims description 31
- 229910052708 sodium Inorganic materials 0.000 claims description 31
- 239000011591 potassium Substances 0.000 claims description 30
- 229910052700 potassium Inorganic materials 0.000 claims description 30
- 238000003860 storage Methods 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 12
- 239000004615 ingredient Substances 0.000 claims description 5
- 150000001413 amino acids Chemical class 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 3
- 229940083542 sodium Drugs 0.000 description 28
- 229960003975 potassium Drugs 0.000 description 27
- 239000000243 solution Substances 0.000 description 16
- 238000001556 precipitation Methods 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- 235000011496 sports drink Nutrition 0.000 description 9
- 150000001875 compounds Chemical class 0.000 description 8
- 235000013405 beer Nutrition 0.000 description 7
- 239000000796 flavoring agent Substances 0.000 description 7
- 235000019634 flavors Nutrition 0.000 description 7
- 238000012360 testing method Methods 0.000 description 6
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 230000001476 alcoholic effect Effects 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 4
- 239000012085 test solution Substances 0.000 description 4
- 239000004381 Choline salt Substances 0.000 description 3
- 235000019417 choline salt Nutrition 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 150000003248 quinolines Chemical class 0.000 description 3
- 159000000000 sodium salts Chemical class 0.000 description 3
- IMAGLCGCHKGGLR-UHFFFAOYSA-M Choline orotate Chemical compound C[N+](C)(C)CCO.[O-]C(=O)C1=CC(=O)NC(=O)N1 IMAGLCGCHKGGLR-UHFFFAOYSA-M 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 235000013373 food additive Nutrition 0.000 description 2
- 239000002778 food additive Substances 0.000 description 2
- 235000015203 fruit juice Nutrition 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 2
- 230000001953 sensory effect Effects 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- 235000011083 sodium citrates Nutrition 0.000 description 2
- 235000013616 tea Nutrition 0.000 description 2
- 235000015192 vegetable juice Nutrition 0.000 description 2
- AEQDJSLRWYMAQI-UHFFFAOYSA-N 2,3,9,10-tetramethoxy-6,8,13,13a-tetrahydro-5H-isoquinolino[2,1-b]isoquinoline Chemical compound C1CN2CC(C(=C(OC)C=C3)OC)=C3CC2C2=C1C=C(OC)C(OC)=C2 AEQDJSLRWYMAQI-UHFFFAOYSA-N 0.000 description 1
- YXUZGLGRBBHYFZ-UHFFFAOYSA-N 2,4-dioxo-1h-pyrimidine-6-carboxylic acid;hydrate Chemical compound O.OC(=O)C1=CC(=O)NC(=O)N1 YXUZGLGRBBHYFZ-UHFFFAOYSA-N 0.000 description 1
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 239000004260 Potassium ascorbate Substances 0.000 description 1
- HLCFGWHYROZGBI-JJKGCWMISA-M Potassium gluconate Chemical compound [K+].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O HLCFGWHYROZGBI-JJKGCWMISA-M 0.000 description 1
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 241001122767 Theaceae Species 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 238000003321 atomic absorption spectrophotometry Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000014171 carbonated beverage Nutrition 0.000 description 1
- 235000013353 coffee beverage Nutrition 0.000 description 1
- CVOQYKPWIVSMDC-UHFFFAOYSA-L dipotassium;butanedioate Chemical compound [K+].[K+].[O-]C(=O)CCC([O-])=O CVOQYKPWIVSMDC-UHFFFAOYSA-L 0.000 description 1
- WPUMTJGUQUYPIV-JIZZDEOASA-L disodium (S)-malate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](O)CC([O-])=O WPUMTJGUQUYPIV-JIZZDEOASA-L 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 235000020094 liqueur Nutrition 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 235000019520 non-alcoholic beverage Nutrition 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 235000007686 potassium Nutrition 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 229960004109 potassium acetate Drugs 0.000 description 1
- 235000019275 potassium ascorbate Nutrition 0.000 description 1
- 229940017794 potassium ascorbate Drugs 0.000 description 1
- 239000011736 potassium bicarbonate Substances 0.000 description 1
- 235000015497 potassium bicarbonate Nutrition 0.000 description 1
- 229910000028 potassium bicarbonate Inorganic materials 0.000 description 1
- 229910000027 potassium carbonate Inorganic materials 0.000 description 1
- 235000011181 potassium carbonates Nutrition 0.000 description 1
- 239000001508 potassium citrate Substances 0.000 description 1
- 229960002635 potassium citrate Drugs 0.000 description 1
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 description 1
- PJAHUDTUZRZBKM-UHFFFAOYSA-K potassium citrate monohydrate Chemical compound O.[K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O PJAHUDTUZRZBKM-UHFFFAOYSA-K 0.000 description 1
- 235000011082 potassium citrates Nutrition 0.000 description 1
- 239000004224 potassium gluconate Substances 0.000 description 1
- 235000013926 potassium gluconate Nutrition 0.000 description 1
- 229960003189 potassium gluconate Drugs 0.000 description 1
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 1
- 229910001414 potassium ion Inorganic materials 0.000 description 1
- PHZLMBHDXVLRIX-UHFFFAOYSA-M potassium lactate Chemical compound [K+].CC(O)C([O-])=O PHZLMBHDXVLRIX-UHFFFAOYSA-M 0.000 description 1
- 239000001521 potassium lactate Substances 0.000 description 1
- 235000011085 potassium lactate Nutrition 0.000 description 1
- 229960001304 potassium lactate Drugs 0.000 description 1
- 239000001415 potassium malate Substances 0.000 description 1
- SVICABYXKQIXBM-UHFFFAOYSA-L potassium malate Chemical compound [K+].[K+].[O-]C(=O)C(O)CC([O-])=O SVICABYXKQIXBM-UHFFFAOYSA-L 0.000 description 1
- 235000011033 potassium malate Nutrition 0.000 description 1
- CONVKSGEGAVTMB-RXSVEWSESA-M potassium-L-ascorbate Chemical compound [K+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] CONVKSGEGAVTMB-RXSVEWSESA-M 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 235000020083 shōchū Nutrition 0.000 description 1
- 235000019265 sodium DL-malate Nutrition 0.000 description 1
- HELHAJAZNSDZJO-OLXYHTOASA-L sodium L-tartrate Chemical compound [Na+].[Na+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O HELHAJAZNSDZJO-OLXYHTOASA-L 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 229960004249 sodium acetate Drugs 0.000 description 1
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 description 1
- 235000010378 sodium ascorbate Nutrition 0.000 description 1
- 229960005055 sodium ascorbate Drugs 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 235000017550 sodium carbonate Nutrition 0.000 description 1
- 239000000176 sodium gluconate Substances 0.000 description 1
- 235000012207 sodium gluconate Nutrition 0.000 description 1
- 229940005574 sodium gluconate Drugs 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 239000001540 sodium lactate Substances 0.000 description 1
- 235000011088 sodium lactate Nutrition 0.000 description 1
- 229940005581 sodium lactate Drugs 0.000 description 1
- 239000001394 sodium malate Substances 0.000 description 1
- 229940074404 sodium succinate Drugs 0.000 description 1
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 description 1
- 239000001433 sodium tartrate Substances 0.000 description 1
- 229960002167 sodium tartrate Drugs 0.000 description 1
- 235000011004 sodium tartrates Nutrition 0.000 description 1
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 description 1
- 235000014214 soft drink Nutrition 0.000 description 1
- 235000015096 spirit Nutrition 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 229960001367 tartaric acid Drugs 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
- 235000015041 whisky Nutrition 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Non-Alcoholic Beverages (AREA)
Description
本発明は、オロト酸を含有する飲料およびその製造方法に関する。より詳細には、本発明は、オロト酸を高濃度で含有してもオロト酸の溶解性や保存安定性に優れ、かつ飲料本来の食味や風味が損なわれない、オロト酸を含有する飲料およびその製造方法に関する。 The present invention relates to a beverage containing orotic acid and a method for producing the same. More specifically, the present invention provides a beverage containing orotic acid, which is excellent in solubility and storage stability of orotic acid even if it contains orotic acid at a high concentration, and does not impair the original taste and flavor of the beverage. It relates to the manufacturing method.
オロト酸には、尿酸値低下作用、持久力向上(酸素消費量低減)、滋養強壮作用、などの健康の維持・増進に有効な様々な作用があることが知られている。 It is known that orotic acid has various actions effective for maintaining and promoting health, such as a uric acid level lowering action, endurance improvement (reduction of oxygen consumption), and nourishing tonic.
オロト酸を効率的かつ簡便に摂取する上で、スポーツドリンクやビール類などの日常的に摂取する飲料に高濃度で配合することは有利である。しかしながら、オロト酸はそれ自体、水への溶解性が低い。例えば０．０２重量％以上の濃度でオロト酸を含有する飲料を調製することは困難である。また、オロト酸は水に一且溶解しても、溶解性が極めて低いため、溶解後、保存している間に沈殿や析出が生じ易い。オロト酸コリン塩のような塩の状態では溶解性は向上するが、塩による風味の低下が懸念される。 In order to ingest orotic acid efficiently and simply, it is advantageous to add it to beverages to be ingested daily such as sports drinks and beers at a high concentration. However, orotic acid itself has low solubility in water. For example, it is difficult to prepare a beverage containing orotic acid at a concentration of 0.02% by weight or more. In addition, even if orotic acid is once dissolved in water, its solubility is very low, and thus precipitation and precipitation are likely to occur during storage after dissolution. In the state of a salt such as choline salt of orotic acid, the solubility is improved, but there is a concern that the flavor may be lowered by the salt.
そのため、オロト酸を高濃度で配合しても、オロト酸が容易に溶解し、保存中に沈殿や析出がなく、しかも風味の良好なオロト酸含有飲料が求められている。 Therefore, there is a demand for orotic acid-containing beverages that can be easily dissolved even when orotic acid is blended at a high concentration, have no precipitation or precipitation during storage, and have a good flavor.
オロト酸の溶解性や保存性を向上させる方法として、塩基性アミノ酸または塩基性ペプチドを用いる方法が知られている（特許文献１）。しかし、アミノ酸やペプチドの添加の好まれない飲料については、これらを用いない別の方法が求められている。 As a method for improving the solubility and storage stability of orotic acid, a method using a basic amino acid or a basic peptide is known (Patent Document 1). However, for beverages in which addition of amino acids or peptides is not preferred, another method that does not use these is required.
本発明の目的は、オロト酸を高濃度で配合しても、オロト酸が容易に溶解し、保存中に
沈殿や析出がなく、しかも飲料本来の食味や風味を損ねることのないオロト酸を含有する飲料、またはその製造方法を提供することにある。
The object of the present invention is to contain orotic acid that dissolves orotic acid easily even if it is blended in a high concentration, does not precipitate or precipitate during storage, and does not impair the original taste or flavor of the beverage. It is to provide a beverage to be produced or a method for producing the same.
本発明は、以下の（１）〜（６）に関する。
（１）オロト酸を０．０２〜０．１重量％、ならびにナトリウムおよびカリウムのいずれか、または両方を０．００５〜０．１３重量％含有する飲料。
（２）飲料中のオロト酸の含有量を０．０２〜０．１重量％とし、ナトリウムおよびカリウムのいずれか、または両方の含有量を０．００５〜０．１３重量％とする工程を含む、オロト酸を含有する飲料の製造方法。
（３）飲料原料を含む液に、ナトリウムおよびカリウムのいずれか、または両方の存在下でオロト酸を溶解させる工程を含む、上記（２）の方法。
（４）オロト酸が、フリー体または水和物である上記（２）または（３）の方法。
（５）飲料中のオロト酸の含有量を０．０２〜０．１重量％とし、ナトリウムおよびカリウムのいずれか、または両方の含有量を０．００５〜０．１３重量％とすることを特徴とする、飲料におけるオロト酸の溶解性および保存安定性を向上させる方法。
（６）オロト酸が、フリー体または水和物である、上記（５）の方法。
The present invention relates to the following (1) to (6).
(1) A beverage containing 0.02 to 0.1% by weight of orotic acid and 0.005 to 0.13% by weight of either or both of sodium and potassium.
(2) including a step of setting the content of orotic acid in the beverage to 0.02 to 0.1% by weight and the content of either or both of sodium and potassium to 0.005 to 0.13% by weight The manufacturing method of the drink containing orotic acid.
(3) The method according to (2) above, comprising a step of dissolving orotic acid in a liquid containing beverage ingredients in the presence of either or both of sodium and potassium.
(4) The method of (2) or (3) above, wherein orotic acid is a free form or hydrate.
(5) The orotic acid content in the beverage is 0.02 to 0.1% by weight, and the content of either or both of sodium and potassium is 0.005 to 0.13% by weight. And a method for improving the solubility and storage stability of orotic acid in a beverage.
(6) The method of (5) above, wherein orotic acid is a free form or a hydrate.
本発明によれば、健康の維持増進のための機能成分として有用なオロト酸を高濃度で含有する飲料が提供される。本発明のオロト酸含有飲料では、長期間保存してもオロト酸が析出することなく、均一で安定な状態が保たれる。また、本発明によれば、オロト酸添加による飲料本来の食味の低下の少ない飲料が提供される。 ADVANTAGE OF THE INVENTION According to this invention, the drink which contains orotic acid useful as a functional component for health maintenance promotion in high concentration is provided. In the orotic acid-containing beverage of the present invention, even when stored for a long period of time, orotic acid does not precipitate, and a uniform and stable state is maintained. Moreover, according to this invention, the drink with little fall of the original taste of the drink by addition of orotic acid is provided.
本発明の飲料は、オロト酸、ならびにナトリウムおよびカリウムのいずれか、または両方を含有する。 The beverage of the present invention contains orotic acid and either or both of sodium and potassium.
本発明に用いられるオロト酸（ウラシル−４−カルボン酸）としては、オロト酸のフリー体（遊離体）、オロト酸の水和物、オロト酸の誘導体、それらの薬理学的に許容される塩があげられるが、飲料への風味の影響の少ないオロト酸のフリー体（遊離体）やその水和物が好ましく用いられる。また、オロト酸の薬理学的に許容される塩として、オロト酸のナトリウム塩またはカリウム塩を用いることができる。 As orotic acid (uracil-4-carboxylic acid) used in the present invention, orotic acid free form (free form), orotic acid hydrate, orotic acid derivative, pharmacologically acceptable salt thereof However, a free form (or free form) of orotic acid or a hydrate thereof, which is less affected by the flavor of the beverage, is preferably used. Moreover, the sodium salt or potassium salt of orotic acid can be used as a pharmacologically acceptable salt of orotic acid.
オロト酸は、微生物由来のもの、化学合成により得られるもの、乳清等食品から抽出したもの等のいずれを用いてもよい。微生物由来のオロト酸としては、例えばUS5,013,656記載の製造方法により取得されるオロト酸等があげられる。また市販のものを用いてもよい。 The orotic acid may be any of those derived from microorganisms, those obtained by chemical synthesis, those extracted from foods such as whey. Examples of orotic acids derived from microorganisms include orotic acids obtained by the production method described in US Pat. No. 5,013,656. Commercially available products may also be used.
本発明の飲料中のオロト酸の含有量は、オロト酸として０．０２〜０．１重量％である。飲料中のオロト酸は、JOURNAL OF AOAC INTERNATIONAL, 87, 1, 116-122 (2004)等に記載の液体クロマトグラフィー等の常法により定量することができる。 The content of orotic acid in the beverage of the present invention is 0.02 to 0.1% by weight as orotic acid. Orotic acid in beverages can be quantified by a conventional method such as liquid chromatography described in JOURNAL OF AOAC INTERNATIONAL, 87, 1, 116-122 (2004).
本発明の飲料には、ナトリウムおよびカリウムの両方が含有されてもよく、ナトリウムのみ、またはカリウムのみが含有されてもよい。 The beverage of the present invention may contain both sodium and potassium, and may contain only sodium or only potassium.
本発明の飲料に用いるナトリウムまたはカリウムは、ナトリウム塩、カリウム塩等のナトリウムまたはカリウムを含む化合物であってもよい。ナトリウム塩またはカリウム塩としては、例えば、クエン酸ナトリウム、クエン酸カリウム、乳酸ナトリウム、乳酸カリウム、炭酸水素ナトリウム、炭酸水素カリウム、炭酸ナトリウム、炭酸カリウム、リンゴ酸ナトリウム、リンゴ酸カリウム、酒石酸ナトリウム、酒石酸カリウム、グルコン酸ナトリウム、グルコン酸カリウム、酢酸ナトリウム、酢酸カリウム、コハク酸ナトリウム、コハク酸カリウム、アスコルビン酸ナトリウム、アスコルビン酸カリウム等があげられる。これらの化合物は、そのまま用いてもよいが、これらの化合物を含有する、一般的に飲料製造に使用される原材料や、麦汁、茶抽出液等の天然由来素材等を用いてもよい。上記のナトリウムまたはカリウムを含む化合物は、１種または２種以上を組み合わせて用いることができる。 Sodium or potassium used in the beverage of the present invention may be a compound containing sodium or potassium such as sodium salt or potassium salt. Examples of the sodium salt or potassium salt include sodium citrate, potassium citrate, sodium lactate, potassium lactate, sodium bicarbonate, potassium bicarbonate, sodium carbonate, potassium carbonate, sodium malate, potassium malate, sodium tartrate, tartaric acid Examples include potassium, sodium gluconate, potassium gluconate, sodium acetate, potassium acetate, sodium succinate, potassium succinate, sodium ascorbate, and potassium ascorbate. These compounds may be used as they are, but raw materials generally used for beverage production, natural ingredients such as wort and tea extract, etc. containing these compounds may be used. The compounds containing sodium or potassium can be used alone or in combination of two or more.
ナトリウムまたはカリウムを含む化合物を用いる場合、これらの化合物は、飲料中で解離していなくてもよい。すなわち、本発明におけるナトリウムまたはカリウムは、ナトリウムイオンまたはカリウムイオンの状態になくてもよい。 When using compounds containing sodium or potassium, these compounds may not be dissociated in the beverage. That is, sodium or potassium in the present invention may not be in the state of sodium ion or potassium ion.
本発明の飲料中のナトリウムおよびカリウムの含有量は、０．００５〜０．１３重量％であり、好ましくは０．００５〜０．１重量％である。該含有量は、ナトリウムおよびカリウムを合わせた含有量であり、ナトリウムまたはカリウムのいずれかが含有されていない場合、すなわちいずれかの含有量がゼロである場合も含まれる。 The content of sodium and potassium in the beverage of the present invention is 0.005 to 0.13% by weight, preferably 0.005 to 0.1% by weight. The content is a combined content of sodium and potassium, and includes the case where either sodium or potassium is not contained, that is, the case where any content is zero.
飲料中のナトリウムおよびカリウムは、原子吸光光度法等の常法により定量することができる。 Sodium and potassium in beverages can be quantified by conventional methods such as atomic absorption spectrophotometry.
本発明の飲料は、飲料中のオロト酸、ならびにナトリウムおよびカリウムの含有量を上記範囲となるように調製する以外は、飲料の通常の製造方法に準じて製造することができる。飲料の製造は、例えば、「改訂新版ソフトドリンクス」（株式会社光琳）を参考とすることができる。 The beverage of the present invention can be produced according to the usual method for producing a beverage, except that the contents of orotic acid and sodium and potassium in the beverage are adjusted to be in the above range. For the production of beverages, for example, “Revised New Version Soft Drinks” (Kokai Co., Ltd.) can be referred to.
飲料中のオロト酸の含有量を０．０２〜０．１重量％とする場合は、飲料中のナトリウムおよびカリウムの含有量を上記範囲となるように調整することにより、オロト酸を溶解させ、保存中の析出を低下させることができる。一方、飲料中のオロト酸の含有量を０．０２重量％未満とする場合は、オロト酸単独で溶解させることができるため、ナトリウムおよびカリウムの含有量は上記範囲とする必要はない。 When the content of orotic acid in the beverage is 0.02 to 0.1% by weight, by adjusting the sodium and potassium content in the beverage to be in the above range, orotic acid is dissolved, Precipitation during storage can be reduced. On the other hand, when the content of orotic acid in the beverage is less than 0.02% by weight, since it can be dissolved by orotic acid alone, the contents of sodium and potassium do not need to be in the above range.
オロト酸を飲料に溶解させる際には、飲料原料を含む液に、オロト酸をナトリウムおよびカリウムのいずれか、または両方の存在下で溶解させることが好ましい。飲料原料を含む液に、オロト酸をナトリウムおよびカリウムのいずれか、または両方の存在下で溶解させる方法としては、オロト酸と、ナトリウムまたはカリウムを含む化合物とを同時に添加してもよいが、より早くオロト酸を溶解させるために、ナトリウムまたはカリウムを含む化合物を溶解させた後に、オロト酸を添加して溶解させる方が好ましい。 When orotic acid is dissolved in a beverage, orotic acid is preferably dissolved in a liquid containing beverage ingredients in the presence of either or both of sodium and potassium. As a method for dissolving orotic acid in a liquid containing beverage ingredients in the presence of either or both of sodium and potassium, orotic acid and a compound containing sodium or potassium may be added at the same time. In order to dissolve orotic acid quickly, it is preferable to dissolve the compound containing sodium or potassium and then add orotic acid to dissolve it.
本発明の飲料のｐＨに特に限定はないが、通常ｐＨ７未満である。 There is no particular limitation on the pH of the beverage of the present invention, but it is usually less than pH 7.
本発明の飲料の具体例としては、スポーツドリンク、ニア・ウォーター、ノンアルコール（ビアテイスト）飲料、果汁入り飲料、野菜汁入り飲料、果汁および野菜汁入り飲料、茶飲料、乳酸菌飲料、炭酸飲料などの非アルコール飲料、ウイスキー、バーボン、スピリッツ、リキュール、ワイン、果実酒、日本酒、中国酒、焼酎、ビール、アルコール度数１％未満のノンアルコールビール、発泡酒、酎ハイなどのアルコール飲料があげられるが、これらに限定されない。 Specific examples of the beverage of the present invention include sports drinks, near water, non-alcoholic (beer taste) beverages, fruit juice beverages, vegetable juice beverages, fruit juices and vegetable juice beverages, tea beverages, lactic acid bacteria beverages, carbonated beverages, etc. Non-alcoholic drinks such as whiskey, bourbon, spirits, liqueur, wine, fruit liquor, Japanese sake, Chinese liquor, shochu, beer, non-alcohol beer with alcohol content of less than 1%, sparkling liquor, and coffee high However, it is not limited to these.
製造された本発明の飲料を充填する容器は、アルミ缶、スチール缶、ＰＥＴボトル、紙
容器など飲料を充填できるものであればいずれであってもよく、また容量についても制限がない。
The manufactured container for filling the beverage of the present invention may be any container that can be filled with a beverage such as an aluminum can, a steel can, a PET bottle, and a paper container, and the capacity is not limited.
以下、実施例によって本発明を更に具体的に説明するが、これらの実施例は本発明を限定するものでない。なお、下記実施例において「％」は「重量％」をいう。 EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples, but these examples do not limit the present invention. In the following examples, “%” means “% by weight”.
（実施例1）
水にオロト酸のみ0.1％となるように添加した溶液と、水に食品添加物のクエン酸三ナトリウム二水和物を0.426％(ナトリウムの含有量は0.1％)となるように溶解した溶液にオロト酸を0.1％添加した溶液を調製し、各溶液をそれぞれ20℃にて30分間攪拌し、オロト酸の溶解性を調べた。
(Example 1)
A solution in which only orotic acid is added to water to a concentration of 0.1% and a solution in which trisodium citrate dihydrate, a food additive, is dissolved in water to a concentration of 0.426% (sodium content is 0.1%) Solutions with 0.1% orotic acid added were prepared, and each solution was stirred at 20 ° C. for 30 minutes to examine the solubility of orotic acid.
その結果、オロト酸のみを添加した溶液はオロト酸の溶け残りが多かったが、クエン酸三ナトリウム二水和物を溶解させた溶液にオロト酸を添加した溶液ではオロト酸はすべて溶解していた。さらに、オロト酸をナトリウムと共に添加した溶液を20℃にて6ヶ月間保存したが、溶液状態に変化は認められなかった。 As a result, the solution to which only orotic acid was added had a lot of undissolved orotic acid, but the solution in which orotic acid was added to the solution in which trisodium citrate dihydrate was dissolved dissolved all orotic acid. . Furthermore, the solution in which orotic acid was added together with sodium was stored at 20 ° C. for 6 months, but no change was observed in the solution state.
（実施例２）
スポーツドリンク(キリンビバレッジ社製) の製造中に、ナトリウムの含有量が0.1％となるようにクエン酸ナトリウムを溶解させた後、オロト酸を、それぞれ、0.02％、0.05％、0.10％、0.15％となるように添加してオロト酸を含有するスポーツドリンクを調製した。これらのスポーツドリンクのオロト酸の溶解性と保存安定性（保存後の溶液状態の変化）を調べた。
(Example 2)
During the production of sports drinks (manufactured by Kirin Beverage), sodium citrate was dissolved so that the sodium content was 0.1%, and then orotic acid was added to 0.02%, 0.05%, 0.10%, and 0.15%, respectively. A sports drink containing orotic acid was prepared. These sports drinks were examined for orotic acid solubility and storage stability (changes in solution state after storage).
オロト酸の溶解性の評価は、溶液を20℃にて30分間攪拌した後、溶け残りの有無を観察することによって行なった。 The solubility of orotic acid was evaluated by stirring the solution at 20 ° C. for 30 minutes and then observing the presence of undissolved residue.
また、保存安定性の評価は、溶液を20℃で6ヶ月保存し、オロト酸の析出の有無を確認することによって行なった。結果を表１に示す。 The storage stability was evaluated by storing the solution at 20 ° C. for 6 months and confirming the presence or absence of orotic acid. The results are shown in Table 1.
保存安定性：○は析出が認められなかったことを示し、×は析出が認められたことを示す。
Storage stability: ◯ indicates that no precipitation was observed, and x indicates that precipitation was observed.
表１に示したとおり、オロト酸を、含有量が0.02〜0.10％となるように添加したスポーツドリンクでは、いずれもオロト酸は良好に溶解し、保存中の析出も認められなかった。また、オロト酸を溶解した溶液の風味は、いずれもスポーツドリンクとして良好なものであった。 As shown in Table 1, in all sports drinks to which orotic acid was added so that the content was 0.02 to 0.10%, orotic acid was well dissolved, and precipitation during storage was not observed. Moreover, the flavors of the solutions in which orotic acid was dissolved were all good as sports drinks.
（実施例３）
水に、カリウムの含有量がそれぞれ、0.005、0.010、0.050、0.100、0.130、0.150%となるように食品添加物のクエン酸三カリウム一水和物を溶解した。その後、それぞれの溶液にオロト酸を含有量が0.05％となるように添加し、実施例２記載の方法に準じて、オロト酸の溶解性と保存安定性（保存後の溶液状態の変化）を調べた。結果を表２に示す。
(Example 3)
The food additive tripotassium citrate monohydrate was dissolved in water such that the potassium contents were 0.005, 0.010, 0.050, 0.100, 0.130, and 0.150%, respectively. Thereafter, orotic acid was added to each solution so that the content was 0.05%. According to the method described in Example 2, the solubility and storage stability of the orotic acid (change in the solution state after storage) were increased. Examined. The results are shown in Table 2.
保存安定性：○は析出が認められなかったことを示し、×は析出が認められたことを示す。
Storage stability: ◯ indicates that no precipitation was observed, and x indicates that precipitation was observed.
表２に示したとおり、カリウムの含有量を0.005〜0.130％としたミネラルウォーターでは、いずれもオロト酸は良好に溶解し、保存中の析出もほぼ認められなかった。 As shown in Table 2, in any mineral water with a potassium content of 0.005 to 0.130%, orotic acid dissolved well, and precipitation during storage was hardly observed.
（実施例４）
水に、ナトリウムの含有量が0.1％となるように炭酸水素ナトリウムを添加した後、オロト酸を含有量が0.1％となるように添加し、溶解させて試験液１を得た。また、試験液１の調製において炭酸水素ナトリウムを添加せず、また、オロト酸のかわりにオロト酸コリン塩を用いる以外は同様の操作を行って試験液２を得た。
Example 4
Sodium bicarbonate was added to water so that the sodium content was 0.1%, and orotic acid was added so that the content was 0.1%, and dissolved to obtain Test Solution 1. Moreover, the test liquid 2 was obtained in the same manner as in the preparation of the test liquid 1, except that sodium hydrogen carbonate was not added and choline salt of orotic acid was used instead of orotic acid.
また、試験液１の調製において水のかわりにスポーツドリンク（キリンビバレッジ社製）を用いる以外は同様の操作を行って試験液３を得た。また、試験液２の調製において、水の代わりにスポーツドリンク（キリンビバレッジ社製）を用いる以外は同様の操作を行って試験液４を得た。
なお、試験液１〜４のいずれにおいても、オロト酸およびオロト酸コリン塩は良好に溶解した。
Moreover, the test liquid 3 was obtained by performing the same operation except using a sports drink (manufactured by Kirin Beverage) instead of water in the preparation of the test liquid 1. Moreover, in preparation of the test liquid 2, the test liquid 4 was obtained by performing the same operation except using a sports drink (made by Kirin Beverage) instead of water.
In any of the test solutions 1 to 4, orotic acid and choline salt of orotic acid dissolved well.
試験液１〜４の、臭いおよび味について、６名のパネラーにより官能評価した。
評価基準は以下のとおりである。
快適に摂取できる：２点
快適まではいかないが自然に飲める：１点
飲むのに問題ない：０点
飲めるがにおいや味が悪い：−１点
摂取できない：−２点
評価の平均点を表３に示す。
The evaluation criteria are as follows.
Can be comfortably ingested: 2 points Cannot be comforted but can be drunk naturally: 1 point No problem to drink: 0 points Can be drunk but bad smell and taste: -1 point Cannot be ingested: -2 points Average score of evaluation is shown in Table 3 Shown in
表３に示すとおり、オロト酸コリン塩を用いて得られた溶液（試験液２および４）は、オロト酸コリン塩に由来する魚臭のような臭いが強く、官能評価の点数は低かった。 As shown in Table 3, the solutions (test solutions 2 and 4) obtained using the orotic acid choline salt had a strong odor like fish odor derived from the orotic acid choline salt, and the sensory evaluation score was low.
（実施例５）
ビール（キリンビール社製）およびノンアルコールビアテイスト飲料（キリンビール社製）の製造中の麦汁に、オロト酸を添加し、オロト酸の含有量が0.06％、ナトリウムとカリウムを合わせた含有量が0.08％のビールおよびノンアルコールビアテイスト飲料を作成した。
(Example 5)
Orotic acid is added to wort during the production of beer (manufactured by Kirin Brewery) and non-alcoholic beer-taste beverage (manufactured by Kirin Brewery Co., Ltd.), the content of orotic acid is 0.06%, and the combined content of sodium and potassium Created 0.08% beer and non-alcoholic beer-taste beverages.
ビール、ノンアルコールビアテイスト飲料におけるオロト酸の溶解性および保存安定性について、実施例２記載の方法に準じて調べた。その結果、いずれも、オロト酸の溶解性および保存安定性は良好であり、風味は摂取するのに問題なくおいしく飲めるものであった。 The solubility and storage stability of orotic acid in beer and non-alcoholic beer-taste beverages were examined according to the method described in Example 2. As a result, the solubility and storage stability of orotic acid were all good, and the flavor was delicious to drink without problems.
本発明は、健康の維持増進に有効なオロト酸を利用する飲料の製造分野において利用できる。 INDUSTRIAL APPLICATION This invention can be utilized in the manufacture field | area of the drink using orotic acid effective in maintenance and promotion of health.
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