JP5829095B2 - Syringe - Google Patents

Description

  The present invention relates to a syringe for injecting a substance to be injected into an injection target region of a living body.

  Regardless of the presence or absence of the injection needle, in the syringe, injection of the injection solution is realized by pressurizing the injection solution. For example, in a needleless syringe that performs injection without going through the injection needle, An explosive may be used as a pressurization source (for example, refer to patent documents 1). In the needleless syringe shown in Patent Document 1, a detonator and pyrotechnics are provided, and a hammer strikes the detonator and the detonator ignites, so that thermal energy is transmitted to the pyrotechnic, and combustion gas is generated there. Pressurization to the injection solution is performed. As a pyrotechnic explosive that generates combustion gas in this way, explosives that generate combustion gas, such as a single explosive based on nitrocellulose, are used.

  Further, for example, Patent Literature 2 discloses a needleless syringe that burns an explosive by applying energy to the explosive by frictional force and pressurizes the injection solution. In this technique, a user using a needleless syringe inserts the part into the explosive by pressing the part of the syringe, and causes the explosive to burn by frictional force generated between the part and the explosive. is there.

JP-T-2004-532049 US Pat. No. 6,537,245

  When using pyrotechnics to burn pyrotechnics as in the prior art, most pyrotechnics based on nitrocellulose generate gaseous gases, but some debris from the detonators. Occur. When the generated combustion residue is mixed with the injection solution, it is undesirably discharged together with the injection solution to the injection target area. In addition, when the explosive is burned by applying friction energy to the explosive according to the pressing operation of the user, a spark generated instantaneously due to the friction becomes an ignition source, but compared with an electric ignition means. Since the power is small and the ignition performance of the gunpowder is not always sufficient, it may be difficult to burn the gunpowder to an extent sufficient to pressurize the injection solution.

  In view of the above-described problems, the present invention provides a syringe capable of suitably burning an explosive (gas generating agent) that generates combustion gas for pressurizing an injection solution and suppressing combustion residues generated during the combustion. The purpose is to do.

  In order to solve the above problems, the present invention employs a configuration in which a gas generating agent for pressurizing an injection solution is burned using an electrode for generating plasma. Unlike the detonator, in principle, the plasma does not generate any combustion residue when it is generated, and combustion energy can be supplied to the gas generating agent while the plasma is generated. Therefore, more suitable injection solution injection can be realized.

Specifically, the present invention relates to a syringe for injecting an injection target substance into an injection target region of a living body, and includes a syringe body, a sealing portion for sealing the injection target substance, and a combustion chamber provided in the syringe body. A combustion chamber containing a gas generating agent that generates a combustion gas for pressurizing the injection target substance enclosed in the enclosure, and igniting the gas generating agent contained in the combustion chamber Igniting means. The ignition means includes a pair of electrodes that generate plasma between the electrodes disposed in the combustion chamber by energy supplied from an external high-voltage power source, and the gas generating agent is contained in the combustion chamber. The electrode is disposed between the pair of electrodes.

  In the syringe according to the present invention, the gas generating agent is combusted in the combustion chamber to generate combustion gas, and pressure is applied to the injection target substance sealed in the sealed portion by the combustion gas, so that the injection target substance Injection to the outside of the syringe is performed. This injection target substance contains a component expected to have an effect inside the injection target region, and the pressure applied by the combustion gas as described above is a driving source at the time of injection. Therefore, if injection by combustion gas pressurization is possible, the state of accommodation of the injection target substance in the syringe and the specific physics of the injection target substance such as liquid, gel-like fluid, powder, granular solid, etc. The form is not asked. The syringe may be of a type that supplies the injection target substance to the injection target region via the needle or a type that does not pass through the needle, depending on the pressure profile obtained by the combustion of the gas generating agent. May be.

  For example, the injection target substance is a liquid, and even if it is a solid, it may be a gel-like solid as long as fluidity enabling injection is ensured. Furthermore, the injection target substance may be in a powder state. The injection target substance includes a component to be sent to the injection target region of the living body, and the component may exist in a state dissolved in the injection target substance, or the component is simply mixed without being dissolved. It may be in the state where it was done. For example, as ingredients to be delivered, there are vaccines for antibody enhancement, proteins for cosmetics, cultured cells for hair regeneration, etc., so that these can be injected into fluids such as liquids and gels. By inclusion, a substance for injection is formed.

  Here, the ignition of the gas generating agent accommodated in the combustion chamber is performed by an ignition means, and the ignition means generates a pair of plasmas by a high voltage applied voltage supplied from a high voltage power supply outside the syringe. Configured as an electrode. Plasma is defined as an ionized gaseous medium in which the concentrations of electrons and positive ions are almost equal and the space charge is zero, and it supplies the gas generant with energy to ignite the gas generant. To get. And the difference between the state where plasma is generated between a pair of electrodes and the state where the detonator is ignited as in the prior art is that the latter is after the pyrotechnic contained in the detonator is ignited and burned. May cause some combustion residue, while the former plasma is only in a state where an ionized gaseous medium is generated in a predetermined space, that is, a space between a pair of electrodes where a gas generating agent is disposed. Therefore, the possibility that some residue is generated by the generation of plasma itself is extremely low.

  Therefore, in the syringe according to the present invention, the gas generating agent can be ignited by the energy from the plasma and can be suitably burned while substantially reducing the combustion residue during operation. As a result, the possibility of contact between the combustion residue and the injection target substance, which has been a concern in the prior art, is reduced. For example, a special structure for avoiding contact of the combustion residue with the injection target substance is simplified. It is also possible to simplify the structure of the syringe such that it can be omitted.

Here, in the syringe, the pair of electrodes may be configured to be able to receive energy supply from the external high voltage power source for a predetermined time. The predetermined time here is a time during which a pair of electrodes receive energy supply from an external high-voltage power supply. In other words, a time during which plasma is generated between the pair of electrodes, that is, a gas generating agent is ignited. It corresponds to the time for supplying energy for the purpose. As described above, in the syringe according to the present invention, it is possible to set the ignition time of the gas generating agent through the time during which the pair of electrodes receive energy supply from the external high voltage power source. As a result, it becomes possible to burn the gas generating agent sufficiently, and it is possible to avoid the occurrence of insufficient pressure applied to the injection target substance due to insufficient combustion.

  Further, in the syringe up to the above, the combustion chamber is formed on one end side of the syringe body by a recess having an open end, and the syringe further closes the open end of the recess with respect to the recess. You may provide the cover part to which it is attached. The pair of electrodes are arranged so as to face each other on the inner wall surface of the recess, and a power line connected to the pair of electrodes is wired so as to be exposed at the opening end side of the recess, When the lid is attached so as to close the opening end of the recess, the lid-side power line connected to the external high-voltage power source is configured to come into contact with the recess-side power line.

  In the syringe configured as described above, a combustion chamber serving as a closed space is formed by attaching the lid to the recess. Therefore, after injecting the injection target substance after burning the gas generating agent once, when another injection target substance is injected again, the injection target substance is sealed again in the sealing portion, and the lid portion is recessed. The gas generating agent may be accommodated in the recess again, and the lid may be attached. By adopting such a configuration, repeated injection of the injection target substance can be easily performed. Further, as described above, when the lid is attached so as to close the opening end of the recess to form the combustion chamber, the lid-side power line connected to the external high-voltage power source is connected to the recess-side power line. It is constituted so that it may be in contact with the portion exposed to the opening end side of the first electrode, and by attaching the lid to the recess without worrying about the wiring to the pair of electrodes, The preparation of energy supply can be completed. Therefore, the convenience of the user of the syringe according to the present invention can be improved.

  In addition, as an aspect of attachment of the lid portion to the recess, the lid portion may be screwed to the recess, or the lid portion is engaged with the recess using an engagement member such as a claw portion. It is preferable that the lid is detachably attached to and fixed to the recess.

  In the syringe according to the present invention, the pair of electrodes may be supplied with energy by an output voltage from a Cockcroft-Walton circuit included in the external high-voltage power supply. The Cockcroft Walton circuit is generally a circuit that makes it possible to obtain a relatively high output voltage with a simple circuit configuration. Of course, a power source including a high voltage generation circuit other than the Cockcroft-Walton circuit may be employed as the power source for the pair of electrodes of the syringe according to the present invention.

  In addition, until now, reference has been made to the ignition configuration of the gas generating agent using plasma generation by a pair of electrodes in order to suppress as much as possible the residue generated from the ignition means in the ignition of the gas generating agent. In addition, it is preferable to suppress as much as possible the generation of residues in the combustion of the gas generating agent. Therefore, nitrocellulose-based explosives can be suitably employed as an example of the gas generating agent.

  Here, the present invention can also be regarded as an injection system including the above-described syringe and an external high-voltage power supply that supplies energy to the pair of electrodes. In the case of the injection system as well, as in the case of the syringe, the gas generating agent can be ignited by the energy from the plasma and burned suitably while substantially reducing the combustion residue during the operation of the syringe. It becomes possible.

  ADVANTAGE OF THE INVENTION According to this invention, it becomes possible to burn suitably the explosive (gas generating agent) which generate | occur | produces the combustion gas for pressurizing injection solution, and to suppress the combustion residue produced in the combustion in a syringe.

It is a figure which shows schematic structure of the syringe which concerns on this invention. It is a figure which shows schematic structure of the gas generating agent vicinity which produces | generates the combustion gas in the syringe shown in FIG. In the syringe shown in FIG. 1, it is a figure which shows arrangement | positioning of the contact of the wiring of a cover part side and a syringe main body side. It is a figure which shows the structure of the power supply for applying a high voltage with respect to the electrode of the injection device shown in FIG. It is a figure which shows the structure for generating the plasma integrated in the injection device shown in FIG.

  Hereinafter, a needleless syringe 1 having no needle (hereinafter simply referred to as “syringe 1”) will be described as an example of a syringe according to an embodiment of the present invention with reference to the drawings. The configuration of the following embodiment is an exemplification, and the present invention is not limited to the configuration of this embodiment.

  Here, FIG. 1A is a cross-sectional view of the syringe 1, and FIG. 1B is a side view of the syringe 1 viewed from the nozzle 4 side for injecting an injection solution. In the following description of the present application, the injection target substances injected into the injection target by the syringe 1 are collectively referred to as “injection solution”. However, this is not intended to limit the content or form of the injected substance. In the injection target substance, the component to be delivered to the skin structure may or may not be dissolved, and the injection target substance can be injected from the nozzle 4 to the skin structure by pressurization. If it exists, the specific form is not ask | required and various forms, such as a liquid, a gel form, and a powder form, are employable. Here, the syringe 1 has a metal syringe body 2, and a central portion of the syringe body 2 is provided with a through hole 14 extending in the axial direction and having a constant diameter along the axial direction. It has been. One end of the through hole 14 communicates with the combustion chamber 9 having a diameter larger than the diameter of the through hole 14, and the other end reaches the nozzle holder 5 side where the nozzle 4 is formed. The combustion chamber 9 is a closed space formed by a concave portion 21 (see FIG. 2 described later) provided on the syringe body 2 side and a metal lid portion 30 attached so as to close the opening end. is there.

  More specifically, the metal piston 6 is disposed in the through hole 14 so as to be slidable in the through hole 14 along the axial direction, and one end thereof is exposed to the combustion chamber 9 side. A sealing member 7 is integrally attached to the other end. The injection liquid ML to be injected by the syringe 1 is accommodated and enclosed in a space formed in the through hole 14 between the sealing member 7 and another sealing member 8. Therefore, the sealing member 7, 8 and the through hole 14 form a sealed portion of the syringe according to the present invention. The sealing members 7 and 8 prevent the injection liquid from leaking out when the injection liquid ML is sealed, and the injection liquid ML can smoothly move in the through hole 14 as the piston 6 slides. It is made of rubber with a thin coating of silicon oil on the surface.

Here, a nozzle holder 5 to which a nozzle 4 for injecting the injection liquid ML is attached is provided on the distal end side (right side in FIG. 1) of the syringe 1. In the syringe 1, the nozzle 4 is a so-called disposable type nozzle and is configured to be detachably held with respect to the nozzle holder 5 so as to be replaced with a new nozzle each time the injection liquid ML is injected. ing. The detailed configuration of the nozzle 4 and the nozzle holder 5 is not a configuration that forms the core of the present invention, and therefore detailed description thereof is omitted. The nozzle holder 5 is fixed to the end surface of the syringe body 2 with a gasket 3 interposed therebetween via a holder cap 13. The holder cap 13 is formed in a hook shape so as to be hooked to the nozzle holder 5, and is fixed to the syringe body 2 with screws. Thereby, the nozzle holder 5 is prevented from dropping from the syringe body 2 due to the pressure applied to the injection liquid ML when the injection liquid ML is ejected.

  In addition, when the nozzle holder 5 is attached to the syringe body 2, a recess 10 that can accommodate the sealing member 8 is formed at a location facing the sealing member 8. The recess 10 has substantially the same diameter as the sealing member 8 and has a depth slightly longer than the length of the sealing member 8. Thereby, when pressure is applied to the piston 6 and the injection liquid ML moves to the distal end side (nozzle 4 side) of the syringe 1 together with the sealing members 7 and 8, the sealing member 8 may be accommodated in the recess 10. It becomes possible. When the sealing member 8 is accommodated in the recess 10, the pressurized injection solution ML is released. In view of this, the flow path 11 is formed so that the released injection liquid ML is guided to the nozzle 4 at a portion of the nozzle holder 5 that contacts the syringe body 2 side. Thereby, the released injection liquid ML is ejected from the nozzle 4 to the injection target through the flow path 11. Moreover, it can avoid that injection | pouring of the injection liquid ML is inhibited by the sealing member 8 because the recessed part 10 has the depth which accommodates the sealing member 8. FIG.

  A plurality of nozzles 4 may be formed on the nozzle holder 5 or one nozzle 4 may be formed. When a plurality of nozzles are formed, a flow path corresponding to each nozzle is formed so that the injection liquid ML released to each nozzle is fed. Furthermore, when a plurality of nozzles 4 are formed, it is preferable that the nozzles are arranged at equal intervals around the central axis of the syringe 1 as shown in FIG. In the present embodiment, the three nozzles 4 in the nozzle holder 5 are arranged at equal intervals around the central axis of the syringe 1. The inner diameter of the nozzle 4 is appropriately set in consideration of the injection object, the injection pressure applied to the injection liquid ML, the physical properties (viscosity) of the injection liquid, the injection depth to the injection object, and the like.

  In the syringe 1 configured as described above, by pressurizing one end surface of the piston 6 exposed to the combustion chamber 9 by the combustion gas generated by the combustion of the gas generating agent 40 disposed in the combustion chamber 9, The injection of the injection liquid ML from the nozzle 4 is executed. That is, when the combustion gas generated from the gas generating agent 40 applies pressure to the injection liquid ML via the piston 6, the injection liquid ML can penetrate the surface of the skin structure as an injection target. Then, the injection liquid ML reaches the inside, and the purpose of injection in the syringe 1 can be achieved.

  Here, the gas generating agent 40 disposed in the combustion chamber 9 generates a combustion gas that provides a sufficient pressure for injection of the injection liquid ML by its own combustion. As an example of the gas generating agent 40, a single base smokeless gunpowder composed of 98% by mass of nitrocellulose, 0.8% by mass of diphenylamine and 1.2% by mass of potassium sulfate can be mentioned. It is also possible to use various gas generating agents that are used in gas generators for airbags and gas generators for seat belt pretensioners. Further, the size, size, and shape of the gas generating agent 40 when placed in the combustion chamber 9 may be appropriately adjusted so as to generate a pressure suitable for injection of the injection liquid ML.

Next, the ignition means of the gas generating agent 40 in the syringe 1 will be described with reference to FIGS. FIG. 2 is a cross-sectional view showing a detailed configuration in the vicinity of the combustion chamber 9 (however, in order to easily understand the configuration of the syringe 1, the cross-sectional location in FIG. 2 includes power lines 22 and 23 connected to the electrode 20. Set to a visible position). 3 is a plan view when the lid 30 is viewed from the syringe body 2 side shown in FIG. 2, and the right view of FIG. 3 is a recess 21 that forms a part of the combustion chamber 9. It is a top view when the one end side of the injection device main body 2 including the opening end of is looked into from the cover part 30 side shown in FIG. Therefore, the lid 30 is screwed to the syringe body 2 side so that the end surface of the lid 30 shown in the left diagram of FIG. 3 closes the opening end of the recess 21 shown in the right diagram of FIG. At this time, as shown in FIG. 2, a seal member 12 such as an O-ring is disposed between the end surface of the lid portion 30 near the threaded portion and the end surface of the syringe body 2. It is devised so that moisture enters the combustion chamber 9 (closed space surrounded by the concave portion 21 and the lid portion 30) from between the main body 2 and ignition of the gas generating agent 40 is not hindered.

  In the present embodiment, a configuration in which plasma is generated in the combustion chamber 9 by the pair of electrodes 20 is employed as the ignition means of the gas generating agent 40 in the syringe 1. A pair of electrodes 20 are disposed on the side wall of the recess 21 forming the combustion chamber 9 (the wall where the end face of the piston 6 is not exposed) so as to face each other, and the gas generating agent 40 is disposed between the electrodes. . One electrode 20 is connected to a power line 22 disposed inside the syringe body 2, and the other electrode 20 is similarly connected to a power line 23 disposed inside the syringe body 2. ing. The power lines 22 and 23 are surrounded by an insulating portion 24 so that the power wires 22 and 23 are insulated from other portions (metal portions) of the syringe body 2. Further, the insulating portion 24 extends to the side wall of the recess 21, and each of the pair of electrodes 20 is similarly insulated from other portions (metal portions) of the syringe body 2 by the insulating portion 24. ing.

  In addition, power lines 32 and 33 are also arranged inside the lid portion 30, and in a state where the lid portion 30 is screwed to the syringe body 2, the power line 32 contacts the power line 22 and the power line 33. Is configured to contact the power line 23. In addition, the periphery of the power supply lines 32 and 33 is also surrounded by the insulating portion 34, and insulation between the power supply lines and insulation between each power supply line and the other portion (metal portion) of the lid portion 30 are maintained. In FIG. 2, the insulating portions 24 and 34 are formed only on a part of the syringe body 2 and the lid portion 30, respectively. However, the range in which each insulating portion is formed is the strength of parts and ease of manufacturing. It can be changed as appropriate based on the above.

  Here, the connection of each power line at the time of attachment of the cover part 30 and the syringe main body 2 will be described based on FIG. In the lid part 30, the end part 32 a of the power supply line 32 and the end part 33 a of the power supply line 33 are each formed in a protruding shape with rounded surfaces, and are exposed on the end face of the lid part 30. The end portion 32a is located on the circumference of the radius r1 from the center of the lid portion 30 (the rotation center when the lid portion 30 is screwed into the syringe body 2), and the end portion 33a is It is located on the circumference of a radius r2 (r2> r1) from the center of the lid 30. On the other hand, in the syringe body 2, the end portion 22 a of the power line 22 and the end portion 23 a of the power line 23 are each formed as an annular band on the end surface of the syringe body 2. Specifically, the end portion 22a is an annular band having a radius r1 from the center of the syringe body 2 (an axis corresponding to the rotation center of the lid portion 30 when the lid portion 30 is screwed into the syringe body 2). The band width is formed and corresponds to the size of the projection of the end portion 32a on the lid portion 30 side. The end 23a is formed as an annular band having a radius r2 (r2> r1) from the center of the syringe body 2, and the band width corresponds to the size of the protrusion of the end 33a on the lid 30 side. Yes.

  Thus, the end portions 22a and 32a related to the power supply lines 22 and 32 that supply power to one electrode 20 are located on the circumference of the radius r1 from the center, and the power supply line 23 that supplies power to the other electrode 20; The end portions 23a and 33a related to 33 are configured to be located on the circumference of the radius r2 from the center, so that even when the lid portion 30 rotates when the lid portion 30 is screwed to the syringe body 2, The end 32a can be in contact with the end 22a, and the end 33a can be kept in contact with the end 23a. Therefore, when the lid 30 is screwed to accommodate the gas generating agent 40 disposed in the recess 21 in the combustion chamber 9, wiring to the pair of electrodes 20 for ignition of the gas generating agent 40 is completed at the same time. This contributes to improving the convenience of the user.

Here, when the lid 30 is screwed onto the syringe body 2 and attached, the power lines 32 and 33 on the lid 30 side are connected to the high voltage power source 50. The high-voltage power supply 50 is a direct-current high-voltage generator that applies a high voltage for plasma generation to a pair of electrodes 20 in the combustion chamber 9, and an example using a Cockcroft-Walton circuit is known. Yes. The DC high voltage generator using this Cockcroft Walton circuit is configured as shown in FIG. In the Cockcroft Walton circuit, a plurality of capacitors C11 to C1N and C21 to C2N are divided into two groups and connected to N stages, respectively, to form a cascade circuit. The connection points of the capacitors of these cascade circuits are sequentially connected by a diode D. Further, the lowest stage of each cascade circuit is the input side, and the AC power supply 51 is connected. A DC high voltage is generated between the high-voltage side output terminal 52 and the low-voltage side output terminal 53, and the DC high voltage is output from these output terminals 52 and 53 as the output voltage Vout.

  Plasma is generated in the combustion chamber 9 by applying a DC high voltage from the high voltage power supply 50 configured in this way between the pair of electrodes 20. Here, plasma generation by the pair of electrodes 20 will be described in detail with reference to FIG. One electrode 200 of the pair of electrodes 20 serves as an electrode for applying a high voltage to generate plasma in the space with the other electrode 210 described later, and has a gas shower plate 201. The gas shower plate 201 blows down a processing gas such as oxygen gas supplied from a processing gas supply pipe 205 (not shown in FIG. 2) and serves as an electrode plate. And a space portion 204 at the center. A high voltage is supplied from the power supply 50 to the gas shower plate 201. Reference numeral 202 denotes an insulating plate of the electrode 200.

  The other electrode 210 is another electrode that applies a high voltage to generate plasma in a space with the electrode 200 at a position facing the electrode 200, and has a surface plate 211. The front plate 211 is a flat electrode plate, is made of aluminum, for example, and is arranged in parallel with the gas shower plate 201. Thereby, the gas shower plate 201 and the front plate 211 are formed in a parallel plate type. The front plate 211 is grounded by a ground wire 213. Reference numeral 212 denotes an insulating plate of the electrode 210.

  When the processing gas is supplied from the processing gas supply pipe 205 and a high voltage is applied to the pair of electrodes 20 from the high voltage power supply 50, plasma is generated in the space between the electrodes and disposed there The generated gas generating agent 40 is ignited by the energy of the plasma. As a result, combustion gas is suddenly generated in the combustion chamber 9, and the piston 6 is pressurized by the pressure, and injection of the injection liquid ML is executed.

  Thus, the combustion residue produced | generated by igniting the gas generating agent 40 using the plasma which generate | occur | produces between a pair of electrodes 20 resulting from the metal particle contained in the detonator and other igniting agents by a prior art Can be suppressed as much as possible. When a detonator or a gas generating agent (also referred to as “pyrotechnics”) is used as a drive source for injecting an injection solution in a needleless syringe such as the syringe 1, the pyrotechnics used Although the amount itself is not so large, the ratio of detonators etc. to gas generating agents in pyrotechnics is not small (for example, 50-80 mg for detonators is 10 mg for gas generating agents). Therefore, by using the plasma as described above to ignite the gas generating agent, the combustion of the detonator and the like is substantially omitted, so the amount of combustion residue generated can be greatly reduced. The possibility of mixing the injection liquid ML and the combustion residue can be reduced.

In addition, in the configuration in which the gas generating agent is ignited by plasma as in the present embodiment, it is possible to greatly reduce the operation noise and vibration at the time of ignition compared to the case of igniting with a detonator or the like as in the prior art. It becomes. In particular, since the vibration during ignition is suppressed, when the user ignites the gas generating agent in a state where the nozzle of the syringe is applied to the injection target area, the syringe does not cause positional displacement due to vibration. Therefore, an appropriate injection becomes possible. The plasma between the electrodes basically continues to be generated while the voltage is continuously applied from the high voltage power source. Therefore, unlike the conventional technology where the gas generant is ignited instantaneously, such as detonator ignition or frictional ignition, the gas generant is burned more reliably, and a sufficient amount of combustion for injection is possible. Gas can be generated reliably. From this point of view, in the syringe 1, the time during which the pair of electrodes 20 receives voltage from the high voltage power supply 50 is set to a predetermined time during which the gas generating agent 40 disposed in the combustion chamber 9 is sufficiently burned. Is preferred.

<Other examples>
According to the syringe 1 according to the present invention, in addition to the case where the above-mentioned injection solution is injected into the skin structure, for example, in the field of regenerative medicine for humans, the cells to be injected and the cultured cells in the scaffold tissue / scaffold It is possible to seed stem cells and the like. For example, as disclosed in JP-A-2008-206477, cells that can be appropriately determined by those skilled in the art depending on the site to be transplanted and the purpose of recellularization, such as endothelial cells, endothelial precursor cells, bone marrow cells, and prebones Blast cells, chondrocytes, fibroblasts, skin cells, muscle cells, liver cells, kidney cells, intestinal cells, stem cells, and any other cells considered in the field of regenerative medicine can be injected with the syringe 1. . More specifically, the liquid (cell suspension) containing the cells to be seeded is accommodated in the through-hole 14 by the sealing members 7 and 8 and is pressurized against it, so that a predetermined site is provided at the site to be transplanted. Cells are injected and transplanted.

  Furthermore, the syringe 1 according to the present invention can also be used for delivery of DNA or the like to cells, scaffold tissue, scaffolds, etc. as described in JP-T-2007-525192. In this case, it can be said that the use of the syringe 1 according to the present invention is more preferable than the case of delivery using a needle because the influence on the cells, the scaffold tissue, the scaffold and the like can be suppressed.

  Furthermore, the syringe 1 according to the present invention is suitable also when various genes, tumor suppressor cells, lipid envelopes, etc. are directly delivered to a target tissue or when an antigen gene is administered to enhance immunity against a pathogen. Used for. In addition, in the field of various disease treatments (fields described in JP2008-508881, JP2010-503616, etc.), immunomedicine (fields described in JP2005-523679, etc.), etc. The syringe 1 can be used, and the field in which the syringe 1 can be used is not intentionally limited.

DESCRIPTION OF SYMBOLS 1 ... Syringe 2 ... Syringe body 4 ... Nozzle 5 ... Holder 6 ... Piston 7, 8 ... Sealing member 9 ... Combustion chamber 10. ... Recess 20 ... A pair of electrodes 21 ... Recesses 22, 23 ... Power lines 22a, 23a ... End 24 ... Insulation part 30 ... Lid 32, 33 ... Power line 32a, 33a ... End 34 ... Insulating part 40 ... Gas generating agent 50 ... High voltage power supply

Claims (6)

A syringe for injecting an injection target substance into an injection target area of a living body,
A syringe body;
An enclosing portion for enclosing the injectable substance;
A combustion chamber provided in the syringe body, and containing a gas generating agent that generates a combustion gas for pressurizing the injection target substance sealed in the sealing portion;
Ignition means for igniting the gas generating agent accommodated in the combustion chamber,
The ignition means includes a pair of electrodes that generate plasma between electrodes disposed in the combustion chamber by energy supplied from an external high-voltage power source,
The gas generating agent is disposed between the pair of electrodes in the combustion chamber ,
The combustion chamber is formed on one end side of the syringe body by a recess having an open end,
The syringe further includes a lid attached to the recess so as to close the open end of the recess,
The pair of electrodes are arranged so as to face each other on the inner wall surface of the recess, and the power line connected to the pair of electrodes is wired so as to be exposed to the opening end side of the recess,
When the lid is attached so as to close the opening end of the recess, the lid-side power line connected to the external high-voltage power supply is configured to come into contact with the recess-side power line.
Syringe. The pair of electrodes is configured to be able to receive energy supply from the external high-voltage power source for a predetermined time.
The syringe according to claim 1. The pair of electrodes is supplied with energy from the output voltage from the Cockcroft-Walton circuit included in the external high-voltage power supply.
The syringe according to claim 1 or claim 2 . The gas generating agent is a nitrocellulose-based explosive,
The syringe according to any one of claims 1 to 3 . The syringe is a needleless syringe that injects an injection target substance into an injection target region without going through an injection needle.
The syringe according to any one of claims 1 to 4 . A syringe according to any one of claims 1 to 5 ,
The external high voltage power supply;
Including an injection system.

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