JP5757563B2 - Cosmetics - Google Patents

Description

  The present invention relates to a cosmetic that can promote turnover of cells constituting the skin.

  Conventionally, various cosmetics have been developed in order to reduce stains and wrinkles that increase with age.

  However, many of them, which supply nutrients that are deficient in the cells that make up the skin, activate the skin cells, and it is difficult to say that cell proliferation and generation change can be performed efficiently. .

  Therefore, in recent years, cosmetics containing a cell growth factor (GF) that promotes proliferation and differentiation of skin cells have been proposed.

  According to the cosmetic containing this cell growth factor, it is said that the proliferation and generation change of skin cells can be efficiently carried out, and the skin can be improved to a youthful skin.

JP 2004-284968 A

  However, it has been difficult to say that the cosmetics containing the conventional cell growth factor still have a sufficient skin improvement effect.

  This invention is made | formed in view of such a situation, Comprising: Compared with the conventional cosmetics, the cosmetics which can induce the turnover of a skin cell efficiently are provided.

In order to solve the above-described conventional problems, the cosmetic according to claim 1, wherein the cell growth factor having a full-length protein form and the cell growth having an incomplete-length protein form having at least a receptor-binding domain are provided. A cosmetic comprising a peptide , wherein the cell growth factor is an epidermal growth factor, and the cell growth peptide is a peptide consisting of 11 to 15 amino acid residues having an epidermal growth factor receptor binding domain. A peptide comprising 8-12 amino acid residues with a fibroblast growth factor receptor binding domain, a peptide comprising 3-7 amino acid residues with a hepatocyte growth factor receptor binding domain, thymosin β4 receptor At least one peptide selected from peptides consisting of 6 to 10 amino acid residues having a body-binding domain was further contained.

Further, in the cosmetic according to claim 2 , in the cosmetic according to claim 1, the content of the cell growth factor is 0.00001 to 10 W / W%, and the content of the cell growth peptide is 0.00001 to 25 W / W. It is characterized by W%.

Moreover, in the cosmetic of Claim 3 , in the cosmetic of Claim 1 or Claim 2 , while containing the polysaccharide which has a water retention function, the moisture content was adjusted to 10-99.9925W / W%. It has a special feature.

The cosmetic according to claim 4 is characterized in that, in the cosmetic according to claim 3 , the content of the polysaccharide is 0.001 to 25 W / W%.

The cosmetic according to claim 5 is characterized in that, in the cosmetic according to claim 3 or 4 , the polysaccharide is a proteoglycan.

The cosmetic according to claim 6 is characterized in that the cosmetic according to any one of claims 1 to 5 contains a phospholipid.

In addition, the cosmetic according to claim 7 is characterized in that the pH is adjusted to 3 to 11 in the cosmetic according to any one of claims 1 to 6 .

In the cosmetic according to claim 1, since a cell growth factor having the form of a full-length protein and a cell growth peptide having a form of an incomplete length protein having at least a receptor binding domain are contained, It is possible to provide a cosmetic that can induce turnover of skin cells more efficiently than a cosmetic. The cell growth factor is an epidermal growth factor, and the cell growth peptide is a peptide consisting of 11 to 15 amino acid residues having an epidermal growth factor receptor binding domain. Furthermore, turnover can be caused effectively. Further, a peptide comprising 8-12 amino acid residues having a fibroblast growth factor receptor binding domain, a peptide comprising 3-7 amino acid residues having a hepatocyte growth factor receptor binding domain, thymosin β4 receptor Since at least one peptide selected from peptides consisting of 6 to 10 amino acid residues having a body-binding domain is further contained, turnover can be more effectively induced on skin cells.

In the cosmetic according to claim 2 , since the content of the cell growth factor is 0.00001 to 10 W / W% and the content of the cell growth peptide is 0.00001 to 25 W / W%, Furthermore, turnover can be caused effectively.

Moreover, in the cosmetic of Claim 3 , since the polysaccharide which has a water retention function was contained and the water content was adjusted to 10-99.9925 W / W%, the application site | part of cosmetics was made over a comparatively long time. It can be kept in a moist state, and the function of cell growth factors and peptides can be prevented as much as possible.

Moreover, in the cosmetic of Claim 4 , since content of the said polysaccharide was 0.001-25W / W%, the application | coating site | part of cosmetics can be kept moist for a comparatively long time.

Further, in the cosmetic according to claim 5 , since the polysaccharide is a proteoglycan, the high retention ability of the proteoglycan can hold other components stably and more effectively. A wet state can be maintained.

Further, in the cosmetic according to claim 6 , since the phospholipid is contained, the cell growth factor and each peptide can be made into a liposome, and the cell growth factor and each peptide can efficiently reach the skin cells. Can do.

Moreover, in the cosmetic of Claim 7 , since pH was adjusted to 3-11, a cell growth factor and each peptide can be made to exist stably in cosmetics.

  The present invention relates to a cosmetic containing a cell growth factor having a form of a full-length protein and a cell growth peptide having a form of an incomplete length protein having at least a receptor binding domain.

  The present inventor has made many years of research on cosmetics, and by incorporating both cell growth factor and cell growth peptide in cosmetics, compared to cosmetics containing either one. It was found that it is possible to induce skin turnover very efficiently.

  Here, the cell growth factor is an epidermal growth factor, and the cell growth peptide is preferably a peptide consisting of 11 to 15 amino acid residues having an epidermal growth factor receptor binding domain.

  Epidermal Growth Factor (EGF) is a human-derived epidermal growth factor, and functions to promote epithelial cell proliferation and gene replication by binding to human epidermal growth factor receptors.

  Specifically, epidermal growth factor is a protein composed of 53 amino acid residues with a molecular weight of 6045 Da in the state of a full-length protein. In the present specification, epidermal growth factor is a concept including an amino acid sequence having one or more of substitution, deletion, addition and insertion of one or several amino acids with respect to the amino acid sequence of epidermal growth factor.

  As the cell growth peptide, a peptide having a molecular weight of 1200 to 2300 Da (hereinafter referred to as “EGF domain peptide”) consisting of 11 to 15 amino acid residues including the region constituting the epidermal growth factor receptor binding domain is preferably used. it can. That is, the cell growth peptide may be an EGF domain peptide that has the ability to bind to and activate the epidermal growth factor receptor.

  Then, by containing these cell growth factors and cell growth peptides in a weight ratio of 1: 5.5 to 1: 6.5, 0.00001 to 10 W / W% and 0.00001 to 25 W / W%, respectively. Thus, it can be made into a cosmetic that can efficiently induce the turnover of skin cells.

  In particular, in the cosmetic according to the present embodiment, both the cell growth factor and the cell growth peptide are formulated, and this is because the reaction of the epidermal growth factor receptor whose reactivity has decreased to the cell growth peptide. This is because the binding domain portion of the cell growth factor to the receptor can be induced to the receptor, and the efficiency of receptor activation can be improved. Therefore, by combining both cell growth factor and cell growth peptide, the productivity of new cells can be improved compared to the case where either cell growth factor or cell growth peptide is added alone. It is considered possible.

  Further, the cosmetic comprises a peptide consisting of 8 to 12 amino acid residues with a fibroblast growth factor receptor binding domain, and 3 to 7 amino acid residues with a hepatocyte growth factor receptor binding domain. Peptides, peptides consisting of 10-14 amino acid residues with insulin-like growth factor receptor binding domain, peptides consisting of 10-14 amino acid residues with transforming growth factor receptor binding domain, thymosin β4 receptor binding You may make it further contain at least any one peptide chosen from the peptide which consists of a 6-10 amino acid residue provided with the domain. In the following description, these peptides are also collectively referred to as “receptor binding peptides”.

  A peptide consisting of 8 to 12 amino acid residues (hereinafter referred to as “FGF domain peptide”) having a fibroblast growth factor receptor binding domain is a fibroblast growth factor (FGF) -like function. It promotes angiogenesis and the promotion of epithelial cell proliferation. The amount of the FGF domain peptide blended in the cosmetic is 0.000001 to 25 W / W%, more preferably 0.00001 to 10 W / W%.

  A peptide consisting of 3 to 7 amino acid residues with a hepatocyte growth factor receptor binding domain (hereinafter referred to as “HGF domain peptide”) exhibits a function similar to hepatocyte growth factor (HGF). It is a peptide that acts as a growth factor for hepatocytes and also has a proliferative effect on many cells such as keratinocytes. The amount of the HGF domain peptide blended in the cosmetic is 0.000001 to 25 W / W%, more preferably 0.00001 to 10 W / W%.

  A peptide consisting of 10 to 14 amino acid residues having an insulin-like growth factor receptor binding domain (hereinafter referred to as “IGF domain peptide”) has an insulin-like growth factor (IGF) -like function. It has a insulin-like action and is also involved in nerve cell proliferation and the like. The amount of the IGF domain peptide blended in the cosmetic is 0.000001 to 25 W / W%, more preferably 0.00001 to 10 W / W%.

  A peptide consisting of 10 to 14 amino acid residues with a transforming growth factor receptor binding domain (hereinafter referred to as “TGF domain peptide”) is a peptide exhibiting a transforming growth factor (TGF) -like function. In addition to acting on the regeneration and repair of damaged cells, it is also involved in the suppression of inflammatory mediators. The amount of the TGF domain peptide to be blended in the cosmetic is 0.000001 to 25 W / W%, more preferably 0.00001 to 10 W / W%.

  A peptide comprising 6 to 10 amino acid residues having a thymosin β4 receptor binding domain (hereinafter referred to as “thymosin β4 domain peptide”) is a peptide exhibiting a function similar to thymosin β4 (fibroblast). It has the effect of proliferating cells and is involved in the synthesis of hyaluronic acid and collagen. The amount of thymosin β4 domain peptide blended in the cosmetic is 0.000001 to 25 W / W%, more preferably 0.00001 to 10 W / W%.

  All of these receptor-binding peptides have a smaller molecular weight than their full-length proteins, and therefore can increase the permeability to skin cells.

  Moreover, the content of these receptor-binding peptides in the cosmetic can be sufficiently exerted the effects of each receptor-binding peptide by setting the content to 0.00001 to 10 W / W%. Moreover, if it is 10-25 W / W%, although the further effect of each receptor binding peptide cannot be anticipated, the moisturizing effect of the skin by a peptide can be improved more.

  By the way, when the above-described cell growth factor, cell growth peptide, and receptor-binding peptide (hereinafter collectively referred to as “cell growth element”) are allowed to act on skin cells, it is desirable to keep the skin moist. .

  When the skin is in a dry state, each cell growth element is difficult to reach the skin cells, and the steric stability of the cell growth element may be lost and deactivated.

  Therefore, it is preferable that the cosmetic contains a polysaccharide having a water retention function and has a water content of 10 to 99.9925 W / W%.

  With such a configuration, the skin can be moistened for a relatively long time, and the function of each cell growth element can be prevented as much as possible.

  Moreover, when the amount of the polysaccharide added to the cosmetic is 0.001 to 25 W / W%, when the cosmetic is applied to the skin, the evaporation of moisture can be moderately suppressed. It is possible to further prevent the function from being inhibited.

  Examples of the polysaccharide capable of exhibiting such a function include sodium hyaluronate, xanthan gum, proteoglycan and the like, and among them, proteoglycan is preferable.

  Proteoglycan has high water retention ability and component retention ability, and can hold cell growth elements and hold it stably, so mixing 0.001-25W / W% in cosmetics moderately suppresses evaporation of moisture. However, the function of the cell growth element can be prevented from being inhibited.

  Moreover, you may contain a phospholipid in cosmetics. Specifically, the cosmetic liquid may be aqueous, and the cosmetic may contain liposomes encapsulating cell growth elements composed of phospholipids.

  With such a configuration, it is possible to make the cell growth element more easily reach the skin cells.

  At this time, by adjusting the pH of the cosmetic to 3 to 11, the liposome and each cell growth element can be stably present in the cosmetic.

  Hereinafter, actual production examples of the cosmetic according to the present embodiment will be described.

  0.5 g of purified water was added to a beaker, 1 mg of EGF as a cell growth factor and 6.5 mg of EGF domain peptide as a cell growth peptide were added, and the mixture was stirred to obtain a uniform solution.

  And the pH of the obtained solution was adjusted to 6.00. Finally, the total weight was adjusted to 100 g with purified water, and the resulting solution was used as a cosmetic according to this embodiment. The moisture content of the prepared cosmetic was 99.9925 W / W%.

  Next, each test using the cosmetic according to the present embodiment (hereinafter referred to as “the present cosmetic”) and the comparative cosmetic is described.

[Comparison test 1]
In this study, it contains only a cell growth factor and a cell growth peptide (EGF and EGF domain peptide in this study) and a cell growth factor (EGF in this study). The improvement effect on the skin of the cosmetic (comparative control cosmetic 1) and the cosmetic (comparative control cosmetic 2) containing only the cell growth peptide (EGF domain peptide in this test) was examined.

  Comparative control cosmetic 1 was obtained by adding 1 mg of EGF as a cell growth factor to 50 g of purified water, stirring to make a uniform solution, and finally preparing 100 g of homogeneous solution with purified water.

  In addition, Comparative Cosmetics 2 was prepared by adding 6.5 mg of EGF domain peptide as a cell growth peptide to 50 g of purified water, stirring to make a uniform solution, and finally preparing a 100 g homogeneous solution with purified water. Obtained.

In this study, 30 women in their 20s and 40s who have marked skin roughness on their cheeks, with 10 as one group, a group using this cosmetic, a group using comparative cosmetic 1, and It was divided into three groups using the comparative cosmetic preparation 2, and 1 ml was applied to the cheek twice a day and used continuously for 1 month. The effect was evaluated after 1 month according to the judgment table. In addition, since this test was implemented by the double blind method, the test subject was not informed about the contents of the cosmetic used. The results are shown in Table 1.

  As shown in Table 1, the present cosmetic material was recognized to have an effect of dramatically improving the skin quality as compared with the comparative control cosmetic materials.

[Comparison test 2]
Next, the test which compares this cosmetics which each differed in composition was done. Specifically, the following cosmetics were prepared and compared.
Cosmetic 1: Add 0.5 g of purified water to a beaker, 1 mg EGF, 6.5 mg EGF domain peptide, 1.5 mg FGF domain peptide, 1.5 mg HGF domain peptide, 1.5 mg IGF domain peptide 1.5 mg of TGF domain peptide and 1.5 mg of thymosin β4 domain peptide were added, respectively, and stirred well to obtain a uniform solution. Next, the obtained solution was added to a uniform solution of 1 g of phospholipid, 20 g of 1,3-butylene glycol, and 5 g of glycerin to make a uniform solution. Then, 50 g of purified water was added to the obtained solution and dispersed therein to obtain a liposome solution. Further, 0.2 g of proteoglycan was added and stirred to adjust the pH to 6.00. Finally, the cosmetic 1 was obtained by adjusting to 100 g with purified water. The moisture content of the prepared cosmetic 1 is 73.7865 W / W%.
Cosmetic 2: prepared by adding 1.5 mg of FGF domain peptide to the present cosmetic used in Comparative Control Test 1. The moisture content of the prepared cosmetic 1 is 99.991 W / W%.
Cosmetic 3: Prepared by further adding 1.5 mg of HGF domain peptide to Cosmetic 2. The moisture content of the prepared cosmetic 1 is 99.9895 W / W%.
Cosmetic 4: Prepared by further adding 1.5 mg of IGF domain peptide to Cosmetic 3. The moisture content of the prepared cosmetic 1 is 99.988 W / W%.
Cosmetic 5: Prepared by further adding 1.5 mg of TGF domain peptide to cosmetic 4. The moisture content of the prepared cosmetic 1 is 99.9865 W / W%.
Cosmetic 6: Prepared by further adding 1.5 mg of thymosin β4 domain peptide to cosmetic 5. The moisture content of the prepared cosmetic 1 is 99.985 W / W%.
Cosmetic 7: Prepared by adding 0.2 g of proteoglycan to the cosmetic used in Comparative Control Test 1. The moisture content of the prepared cosmetic 1 is 99.7925 W / W%.
Cosmetic 8: 1 mg of EGF and 6.5 mg of EGF domain peptide, 0.5 g of purified water is added to make a homogeneous solution, and the solution is made up of 1 g of phospholipid, 20 g of 1,3-butylene glycol, and 5 g of It added to the uniform solution of glycerol and the mixed solution was obtained. And the purified water was added and disperse | distributed to the obtained mixed solution, and it was set as the 100g liposome solution. This cosmetic 8 is prepared so as to contain liposomes. The moisture content of the prepared cosmetic 1 is 73.9925 W / W%.

About these cosmetics 1-8, 80 women in their 20s and 40s who have marked skin roughness on the cheeks are divided into 8 groups of 10 people per group. It was used for months, and its effect was evaluated according to the judgment table after one month. In addition, since this test was implemented by the double blind method, the test subject was not informed about the contents of the cosmetic used. The results are shown in Table 2.

  As shown in Table 2, the cosmetic 1 was found to have an effect of dramatically improving the skin quality as compared with the other cosmetics 2 to 8.

  Moreover, when the progress was confirmed during the continuation of use, the cosmetics 2 to 6 and 8 showed a delay in improving the skin quality as compared with the cosmetics 1 and 7. This was thought to be because proteoglycan as a polysaccharide causing moisturizing effect was not included. However, the cosmetic 8 showed no noticeable delay as much as the cosmetics 2 to 6 in the skin quality improving effect. This was thought to be because the turnover of skin cells was effectively promoted by liposomes formed from phospholipids.

  In addition, paying attention to the present cosmetics (see Table 1) used in Comparative Control Test 1 and the cosmetics 2 to 6 shown in Table 2, in addition to EGF and EGF domain peptides, cosmetics added with FGF domain peptides 2 was effective in improving the skin quality. Although not shown in the table, such a result was not derived in a preliminary test using a cosmetic material to which only the FGF domain peptide was added. It was suggested that a further synergistic effect can be obtained when the domain peptide coexists with EGF and the EGF domain peptide.

  As described above, the cosmetic according to the present embodiment contains a cell growth factor having a full-length protein form and a cell growth peptide having an incomplete-length protein form having at least a receptor binding domain. Therefore, it is possible to provide a cosmetic that can induce turnover of skin cells more efficiently than conventional cosmetics.

  Finally, the description of each embodiment described above is an example of the present invention, and the present invention is not limited to the above-described embodiment. For this reason, it is a matter of course that various modifications can be made in accordance with the design and the like as long as they do not depart from the technical idea according to the present invention other than the embodiments described above.

Claims (7)

A cosmetic comprising a cell growth factor having a form of a full-length protein and a cell growth peptide having a form of an incomplete length protein having at least a receptor binding domain ,
The cell growth factor is an epidermal growth factor, and the cell growth peptide is a peptide consisting of 11 to 15 amino acid residues having an epidermal growth factor receptor binding domain;
Peptide consisting of 8-12 amino acid residues with fibroblast growth factor receptor binding domain, peptide consisting of 3-7 amino acid residues with hepatocyte growth factor receptor binding domain, thymosin β4 receptor binding A cosmetic comprising at least one peptide selected from peptides consisting of 6 to 10 amino acid residues having a domain. Cosmetic according to claim 1, wherein the content of the cell growth factor and 0.00001~10W / W%, characterized in that the content of the cell growth peptide 0.00001~25W / W%. The cosmetic according to claim 1 or 2 , wherein a polysaccharide having a water retention function is contained and the water content is adjusted to 10 to 99.9925 W / W%. The cosmetic according to claim 3 , wherein a content of the polysaccharide is 0.001 to 25 W / W%. The cosmetic according to claim 3 or 4 , wherein the polysaccharide is a proteoglycan. The cosmetic according to any one of claims 1 to 5 , wherein a phospholipid is contained. The cosmetic according to any one of claims 1 to 6, wherein the pH is adjusted to 3 to 11.