JP5565917B2 - Decreased appetite fragrance composition - Google Patents

Description

  The present invention relates to an appetite-reducing fragrance composition, and more specifically, by extracting an appetite-suppressing component from a large number of herbal medicines, mixing it with ethanol at an appropriate ratio, and making it volatile and liquefied, so that only the scent is smelled at any time It is related with the appetite decreasing fragrance | flavor composition which suppressed appetite and enabled it to implement | achieve a diet.

  In general, fragrances provide a refreshing sensation to the indoor atmosphere with a scent, make the feeling refreshing and bright, and give comfort, or to deodorize odors in places where odors such as refrigerators tend to occur. Used as a deodorant. For this reason, a fragrance | flavor is requested | required by a refreshing feeling, refreshingness, pleasure, etc. In some cases, it may be requested | required to the feeling of stability of a mind and body, and a calming effect.

  However, conventional fragrances are composed of some natural fragrances and synthetic fragrances, and have the main purpose of releasing the fragrance of the fragrances. And the fragrance component of the fragrance may cause a more severe malodor, and in particular, in the case of a synthetic fragrance, it may cause confusion and headache depending on the intensity of the odor.

  For this reason, in recent years, there has been an increasing number of attempts to dry fragrant plants (herbs, herbal medicines, etc.) and use them as fragrances in order to provide natural natural fragrances. The present condition is that the fragrance | flavor composition which provides this has not been developed yet.

  In addition, the recent development of the national economy is striking, and obesity has become a factor that threatens the health of the people as they consume large amounts of processed foods with high energy content and exercise amount decreases. Obesity not only impresses people with an unpleasant appearance and inconveniences their activities, but obese people have diabetes, arteriosclerosis, hypertension, heart disease, liver disease, gallstones compared to normal people It is reported to be short-lived due to the high incidence of symptoms, ventilation, kidney disease and the risk of surgery. Obesity is therefore a life problem for those over the age of middle age and a serious problem for young women for cosmetic reasons.

  At present, diet therapy, exercise therapy, behavioral therapy, drug therapy, surgical therapy, and the like have been proposed as treatment methods for obesity. Above all, although diet therapy is the most basic treatment for obesity treatment, many patients complain that it is difficult to adjust the amount of food. Therefore, aroma therapy is used as an auxiliary method for suppressing appetite, but there is no data to prove its effect yet.

  Aroma therapy is one of the natural treatment medicines that uses natural perfume oils to tune human natural healing ability and activate immune system to treat and prevent diseases, and depending on the materials used. It is a therapy with various effects and is widely used so that its use cannot be restricted. In other words, it is not only used as a treatment for physical and mental health, but also as a raw material for cosmetics, and recently it is also used as a part of interior decorations.

  Therefore, the present invention has been devised in order to improve the above-mentioned conventional problems, and the purpose thereof is a natural cure for obesity that is the largest cause of adult disease and also contributes to cosmetic inhibition. In order to enhance the ability and activate the immune system, it is an object of the present invention to provide an appetite-reducing fragrance composition capable of reducing the body weight by producing an effect of suppressing appetite in the human body through natural herbal medicine.

  In addition, another object of the present invention is to provide an appetite-reducing fragrance composition that provides a stable scent to the user and allows the diffused scent to float around a place where the fragrance is provided. is there.

  Furthermore, another object of the present invention is to prevent rot caused by moisture by maintaining dryness for a long time without causing rot due to various fine substances even when the appetite-reducing fragrance composition is used over a long period of time. An appetite-reducing fragrance composition is provided.

  In order to achieve the above object, the present invention provides licorice extract, bellflower extract, large yellow extract, light load extract, birch extract, nematode extract, koji extract, toki extract, windproof extract, coconut extract, eel extract, ginger extract. It is characterized by consisting of a wind-proof tunseongsan medicinal material extract such as renren extract and white birch extract and ethanol.

  More preferably, the present invention relates to 0.5 to 1 parts by weight of licorice extract, 0.5 to 1 part by weight of bellflower extract, 0.3 to 0.7 parts by weight of Daio extract, 90 parts by weight of ethanol, and 90 parts by weight of ethanol. 0.3-0.7 parts by weight, white birch extract 0.5-1 parts by weight, senkyu extract 0.3-0.7 parts by weight, koji extract 0.3-0.7 parts by weight, toki extract 0.3 -0.7 part by weight, windproof extract 0.3-0.7 part by weight, eggplant extract 0.3-0.7 part by weight, ougon extract 0.5-1 part by weight, ginger extract 0.3-0. It contains 7 parts by weight, 0.3 to 0.7 parts by weight of continuous extract, and 0.3 to 0.7 parts by weight of birch extract.

  The appetite-reducing fragrance composition according to the present invention provides an effect of weight loss while maintaining health while exerting the effect of suppressing the appetite of the natural herbal medicine of Fufutsu Seisanhara soaking into the human body. To do.

  In addition, the present invention provides an effect that greatly improves the health of the human body due to the scent of the herbal medicine, by using the herbal medicine that makes the human body healthy as a constituent component.

  Furthermore, the appetite-reducing fragrance composition according to the present invention provides the user with a stable scent and provides the effect that the emitted scent drifts around the place where the fragrance is provided for a long time.

  Furthermore, the reduced appetite fragrance composition according to the present invention does not cause spoilage due to various microorganisms even when used over a long period of time, and maintains the drying property of the decreased appetite fragrance composition for a long time. Provides the effect of preventing corruption.

The graph which shows the change of Leptin positive cell expression of a test object group. The graph which shows the change of NPY positive cell expression of an experiment object group. The graph which shows the change of the short-term memory ability of a test object group. The graph which shows the change of BDNF protein expression of an experiment object group.

  The present invention includes licorice extract, bellflower extract, large yellow extract, light load extract, white camellia extract, white cucumber extract, persimmon extract, toki extract, windproof extract, eggplant extract, Japanese oak extract, ginger extract, ream extract, white birch extract, etc. It is characterized by consisting of Fenghuang Du Seongsan Pharmaceutical Extract and ethanol.

Hereinafter, an appetite reducing fragrance composition according to the present invention will be described with reference to Examples and the accompanying drawings.
Experiments were carried out by varying the content of the constituents according to the intensity of the scent of the herb used in one embodiment of the present invention, and the ratio of the constituents at which the scent was optimal could be confirmed. . As a result, the reduced appetite fragrance composition according to the present invention is 0.5 to 1 part by weight of licorice extract, 0.5 to 1 part by weight of bellflower extract, and 0.3 to 0 of Daio extract with respect to 90 parts by weight of ethanol. 0.7 parts by weight, 0.3 to 0.7 parts by weight of a light load extract, 0.5 to 1 part by weight of a white birch extract, 0.3 to 0.7 parts by weight of a cucumber extract, 0.3 to 0.7 parts by weight of a koji extract It is characterized by including.

The present invention relates to an appetite-reducing fragrance composition, and the specific steps for obtaining it are performed according to the following procedure.
1) drying, mixing and crushing each of licorice, bellflower, large yellow, light load, white birch, nematode, and persimmon;
2) placing the mixture in a supercritical extraction tank and supplying a supercritical fluid;
3) maintaining the pressure in the extraction tank at 400 atm and raising the temperature inside the extraction tank to 40 ° C. at a rate of 0.5 ° C./min;
4) Maintaining the temperature and pressure of the extraction tank at 30 to 50 ° C. and 200 to 500 atmospheres for 5 to 7 hours, and then reducing the pressure to 1 atmosphere;
5) obtaining a reduced appetite fragrance composition by dissolving and removing the wax component and the non-volatile substance contained in the mixture that has undergone Steps 1 to 4 in a solvent such as ethanol;
including.

Hereinafter, the step of extracting the appetite-reducing fragrance composition according to the present invention will be described in detail.
First, each licorice, bellflower, large yellow, light load, birch, nematode, and persimmon are completely dried and mixed, and then finely pulverized using a pulverizer.
The material that has undergone the above fine pulverization step is put into a supercritical extraction tank, and carbon dioxide is supplied to the extraction tank using a high-pressure gas pump to increase the pressure. When the pressure in the extraction tank reaches 400 atm, the supply of carbon dioxide is stopped. At this time, the temperature inside the extraction tank is maintained at 25 ° C to 35 ° C. This is because the pressurization pump and the extraction tank are connected by a long pipe (1 m), so the area close to the pressurization pump is heated, but the temperature inside the extraction tank does not actually change. is there.

After the step of supplying carbon dioxide, the temperature inside the extraction tank is raised to 65 ° C. at a rate of 0.5 ° C./min using a temperature controller. The pressure in the extraction tank due to the temperature rise is maintained at 200 to 500 atm while partially discharging carbon dioxide using a pressure regulator attached to the outlet of the extraction tank.

  After passing through the steps of increasing the temperature and pressure, the temperature and pressure of the extraction tank are maintained at 30 to 50 ° C. and 200 to 500 atmospheres for 5 to 7 hours. The high-pressure gas pump is restarted and carbon dioxide is continuously supplied to the extraction tank at a flow rate of 20 L / min. At the same time, the temperature and pressure of the extraction tank are kept constant while discharging the extract using a pressure regulator attached to the outlet side. Thereafter, the released extract is decompressed to 1 atm to release carbon dioxide to the atmosphere, and the active ingredients of the aromatic essential oil extract are collected together in the collector. An extract is obtained by performing the above process for 12 hours.

The reduced appetite fragrance composition according to the present invention is characterized by mixing 50 g of natural essential oil extract obtained by the above extraction method and 450 g of ethanol.
In addition, the appetite-reducing fragrance composition according to another embodiment of the present invention includes 0.5 to 1 part by weight of licorice extract, 0.5 to 1 part by weight of bellflower extract, and toki extract with respect to 90 parts by weight of ethanol. 0.3-0.7 parts by weight, Dai-yellow extract 0.3-0.7 parts by weight, light-loading extract 0.3-0.7 parts by weight, windproof extract 0.3-0.7 parts by weight, white birch extract 0. 5 to 1 part by weight, Senkyu extract 0.3 to 0.7 part by weight, Eggplant extract 0.3 to 0.7 part by weight, Ogon extract 0.5 to 1 part by weight, Ginger extract 0.3 to 0.7 part It is characterized by containing 0.3 parts by weight, 0.3 to 0.7 parts by weight of a continuous extract, 0.3 to 0.7 parts by weight of a white extract, and 0.3 to 0.7 parts by weight of a white extract.

Yet another embodiment of the present invention relates to an appetite-reducing fragrance composition containing 14 types of anti-air fragrance medicine extracts, and the steps for obtaining this are performed according to the following procedure. Is called.
1) drying, mixing and crushing each of licorice, bellflower, toki, large yellow, light load, windproof, white birch, nematode, eggplant, hornon, ginger, ream, white birch, cocoon;
2) placing the mixture in a supercritical extraction tank and supplying a supercritical fluid;
3) maintaining the pressure in the extraction tank at 400 atm and raising the temperature inside the extraction tank to 45 ° C. at a rate of 0.5 ° C./min;
4) Maintaining the temperature and pressure of the extraction tank at 30 to 50 ° C. and 200 to 500 atmospheres for 5 to 7 hours, and then reducing the pressure to 1 atmosphere;
5) obtaining a reduced appetite fragrance composition by dissolving and removing the wax component and non-volatile substance contained in the mixture that has undergone Steps 1 to 4 in a solvent such as ethanol;
including.

  First, each licorice, bellflower, Toki, Daihuang, light load, windproof, white cocoon, nematode, eggplant, owlon, ginger, reeds, white birch, and persimmon are completely dried and mixed, and then fined using a pulverizer. Grind into.

The material that has undergone the fine pulverization step is put into a supercritical extraction tank, and carbon dioxide is supplied to the extraction tank using a high-pressure gas pump to increase the pressure. When the pressure in the extraction tank reaches 400 atm, the supply of carbon dioxide is stopped.
At this time, the temperature inside the extraction tank is maintained at 25 to 35 ° C. This is because the pressurization pump and the extraction tank are connected by a long pipe (1 m), so the area close to the pressurization pump is heated, but the temperature inside the extraction tank does not actually change. is there.

  After passing through the step of supplying carbon dioxide, the temperature inside the extraction tank is increased to 65 ° C. at a rate of 0.5 ° C./min using a heat controller surrounding the outside of the extraction tank using a temperature controller. Raise the temperature. The pressure in the extraction tank due to the temperature rise is maintained at 200 to 500 atmospheres while partially discharging carbon dioxide using a pressure regulator attached to the outlet of the extraction tank.

  After passing through the steps of increasing the temperature and pressure, the temperature and pressure of the extraction tank are maintained at 30 to 50 ° C. and 200 to 500 atmospheres for 5 to 7 hours. The high-pressure gas pump is restarted and carbon dioxide is continuously supplied to the extraction tank at a flow rate of 20 L / min. At the same time, the temperature and pressure of the extraction tank are kept constant while discharging the extract using a pressure regulator attached to the outlet side. Thereafter, the released extract is decompressed to 1 atm to release carbon dioxide to the atmosphere, and the active ingredients of the aromatic essential oil extract are collected together in the collector. An extract is obtained by performing said process for 10 to 12 hours.

  The reduced appetite fragrance composition according to the present invention is characterized by mixing 50 g of natural essential oil extract obtained by the above extraction method and 450 g of ethanol.

The reason why the ratio of the Chinese medicine material of the present invention is determined as described above is because the odor inherent to the Chinese medicine material is taken into account, and this will be described below.
That is, the ratio of the Chinese medicine material of the present invention is 0.3 to 0.7 parts by weight of the thin load extract and strawberry extract and 0.5 to 1 part of the licorice extract in order to make the user feel refreshed. In order to reduce the heat that causes obesity in the weight part, the ratio of large yellow extract and nematode extract is 0.3-0.7 parts by weight, and the ratio of white cocoon extract is 0 to prevent the body's weakness due to decreased appetite The ratio of ginger extract and white cocoon extract is 0.3 to 0.7 parts by weight in order to smooth the metabolic activity of the body in 0.5 to 1 part by weight.

  That is, when configuring the herbal medicine out of the range of the ratio of the herbal medicine, the fragrance is exhaled excessively, the effect of calming the heat is not obtained so much, the vomiting symptom due to the odor inherent to the herbal medicine, etc. There was a problem of causing.

  Even in the case of the above-mentioned herbal medicines, when separating each herb and using a single incense as it is, it is too easy to diverge and its fragrance easily disappears. The herbal fragrance composition according to the present invention is special to the user by blending and preventing the fragrance from being easily diffused by blending various fragrances, and blending the components in different amounts. A fragrance that provides a refreshing sensation, refreshingness, and pleasure in a stable manner is emitted.

  Next, the above-described herbal fragrance composition according to the present invention includes licorice extract, bellflower extract, toki extract, large yellow extract, light load extract, windproof extract, white birch extract, nematode extract, eggplant extract, eel extract, ginger extract. The herb fragrance mentioned above has a strong divergence property, so the fragrance does not get jammed around the place where the herbal fragrance composition is installed, and in a wider space Try to diverge. In order to suppress such fragrance divergence and to congest the fragrance as it is without fragrance radiating from around the place where the herbal fragrance composition is provided, Dai-Yo is 0.2 to 0.5 to 90 parts by weight of ethanol. It is preferable to further blend parts by weight. In addition, the divergence of the fragrance of the herbal fragrance composition by large yellow can be suppressed by setting the weight ratio of the components of the large yellow extract to 0.3 to 0.7 parts by weight.

  Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to examples and comparative examples. However, these examples are for explaining the present invention, and the scope of the present invention is not limited to these examples.

[Example 1] Manufacture of a reduced appetite fragrance composition Licorice 80g, bellflower 80g, large yellow 60g, thin load 40g, white cocoon 80g, nematode 40g, cocoon 40g was dried, mixed and pulverized mixture was put into a supercritical extraction tank After that, carbon dioxide was supplied to the extraction tank using a high-pressure gas pump, and when the pressure reached 450 atm, the supply of carbon dioxide was stopped. Using the temperature controller, the temperature inside the extraction tank was raised to 65 ° C. at a rate of 0.5 ° C./min through a hot wire surrounding the outside of the extraction tank. The temperature and pressure of the extraction tank were maintained at 65 ° C. and 450 atm for 6 hours while partially discharging carbon dioxide using a pressure controller attached to the outlet of the extraction tank. Thereafter, the high-pressure gas pump was restarted and carbon dioxide was continuously supplied to the extraction tank at a flow rate of 20 L / min. At the same time, the temperature and pressure of the extraction tank are maintained at 65 ° C. and 450 atm for 6 hours while discharging the extract using a pressure regulator attached to the outlet side. The high-pressure gas pump was restarted and carbon dioxide was continuously supplied to the extraction tank at a flow rate of 20 L / min. At the same time, the temperature and pressure of the extraction tank were kept constant while discharging the extract using a pressure regulator attached to the outlet side.

  Next, the released extract was decompressed to 1 atm to release carbon dioxide to the atmosphere, and the active ingredients of the reduced appetite fragrance composition were collected together in the collector. The above process was carried out for 12 hours to obtain an extract, and then the extraction operation was completed to obtain 51.3 g of natural essential oil, and 50 g of natural essential oil was dissolved in 450 g of this natural essential oil. When the degree of appetite reduction of the reduced appetite fragrance composition produced by the above method was tested, the results shown in Table 1 below were obtained.

  Table 1 above is an experiment in which a group that has inhaled fragrance and a group that has not been inhaled are tested separately, and each group displays a VAS test indicating whether or not appetite has decreased during each time period. As described above, it was determined that the appetite decreased more in the inhalation group than in the non-inhalation group. It can be seen that the non-inhaled group shows a constant appetite regardless of the passage of time, whereas the group inhaled with the composition produced according to the present invention gradually decreases in appetite over time.

  In addition, in order to ensure the accuracy of this experiment, inhalers who participated in the experiment were prohibited from taking other substances other than water for 1 hour before inhaling the fragrance to block the appetite of other factors. During the 60 minutes of the experiment, he demanded that he did not perform any activities that required concentration, such as reading, the Internet, or watching TV, for the accuracy of appetite evaluation. Then, since the fragrance composition stock solution may be too irritating when it directly hits the skin, only about 2 to 4 drops were dried and inhaled.

  For this reason, in such an experimental result, only the fragrance composition is an influencing factor, and that the effect of reducing appetite in the inhalation group was obtained, the present invention reduces the appetite and obtains a diet effect It is suggested that it contributes to.

[Example 2] Manufacture of reduced appetite fragrance composition 100 g of licorice, 100 g of bellflower, 80 g of large yellow, 60 g of light load, 100 g of white birch, 60 g of nematode, 60 g of cocoon are prepared in the same manner as in Example 1 Manufactured. When the degree of appetite reduction of the reduced appetite fragrance composition produced by the above method was tested, the results shown in Table 2 below were obtained.

  The conditions for carrying out the experiment as in Example 1 were the same. Examining the results, as is apparent from Table 2 above, the effect of reducing appetite is slightly increased by the increase in the proportion of the ingredients of Chinese herbal medicine. In addition, as the ratio of light load and cocoon extract increased, the inhaler felt refreshed, and no abnormal signs were observed in the subject after the experiment.

[Example 3] Manufacture of reduced appetite fragrance composition 50 g of liquorice, 50 g of bellflower, 30 g of Toki, 37 g of large yellow, 30 g of light load, 30 g of windbreak, 50 g of white shark, 30 g of senkyu, 30 g of eggplant, 50 g of ginger, 30 g of ginger, 30 g of ream In the same manner as in Example 1, an appetite-reducing fragrance composition was produced using 30 g of white candy and 30 g of candy.
When the degree of appetite reduction of the reduced appetite fragrance composition produced by the above method was tested, the results shown in Table 3 below were obtained.

  The conditions for carrying out the experiment as in Example 1 were the same. Examining the results, Tokachi, which was not included in Example 1, windbreaks, etc., strengthened the effects of licorice and calmed the heat of the body, the effect of reducing appetite was further increased, and ginger and By including the glaze extract, the effect of supplementing the body was also obtained.

[Example 4] Manufacture of reduced appetite fragrance composition Licorice 60 g, bellflower 60 g, Toki 40 g, Daio 50 g, light load 40 g, windproof 40 g, white cocoon 60 g, nematode 40 g, eggplant 40 g, oxon 60 g, ginger 40 g, ream 40 g In the same manner as in Example 1, an appetite-reducing fragrance composition was produced using 40 g of white candy and 40 g of candy.
When the degree of appetite reduction of the reduced appetite fragrance composition produced by the above method was tested, the results shown in Table 4 below were obtained.

  The conditions for carrying out the experiment as in Example 1 were the same. Examining the results, Tokachi, which was not included in Example 1, windbreaks, etc., strengthened the effects of licorice and calmed the heat of the body, the effect of reducing appetite was further increased, and ginger and By including the glaze extract, the effect of supplementing the body was also obtained.

[Comparative Example 1]
An appetite-reducing fragrance composition was produced in the same manner as in Example 1 except that 60 g of licorice, 60 g of bellflower, 40 g of large yellow, 20 g of light load, 60 g of white cocoon, 20 g of nematode, and 20 g of koji. When the degree of appetite reduction of the reduced appetite fragrance composition produced by the above method was tested, the results shown in Table 5 below were obtained.

  The conditions for this experiment are also the same as in Example 1. Examining the results, it can be seen that the appetite reduction effect of the group inhaling the fragrance and the group not so is not so great. The weaker the ingredients of the herbal medicine, the less the odor inherent to the herbal medicine becomes less discomforting to the user. I understand that there is not much.

[Comparative Example 2]
An appetite-reducing fragrance composition was prepared in the same manner as in Example 1 using 120 g of licorice, 120 g of bellflower, 100 g of large yellow, 80 g of light load, 120 g of white camellia, 80 g of nematode, and 80 g of salmon.
When the degree of appetite reduction of the reduced appetite fragrance composition produced by the above method was tested, the results shown in Table 6 below were obtained.

  The conditions for this experiment are also the same as in Example 1. Examining the results, it can be seen that the appetite of the group that inhaled the fragrance was reduced compared to the group that did not inhale the fragrance. However, as the component ratio of the herbal medicines increased, the side effects that caused the vomiting and nausea of the user were caused due to excessive release of the fragrance inherent to the herbal medicines.

[Comparative Example 3]
Licorice 30g, bellflower 30g, Toki 20g, light yellow 27g, light load 20g, windproof 20g, white cocoon 30g, nematode 20g, coconut 20g, ogon 30g, ginger 20g, reed 20g, white cocoon 20g, cocoon 20g of Example 1 A reduced appetite fragrance composition was produced in the same manner as the method.
When the degree of appetite reduction of the appetite-reducing fragrance composition produced by the above method was tested, the results shown in Table 7 below were obtained.

  The conditions for carrying out the experiment as in Example 1 were the same. As a result of examining the results, the proportion of the herbal medicine is small, and the fragrance inherent in the herbal medicine is not strong, so it did not cause vomiting, but the effect of the loss of appetite was also compared with Example 1. I found that there was not much.

[Comparative Example 4]
Licorice 70g, bellflower 70g, Toki 50g, large yellow 47g, light load 50g, windproof 50g, white cocoon 70g, nematode 50g, eggplant 50g, oxon 70g, ginger 50g, reed 50g, white cocoon 50g, salmon 50g of Example 1 A reduced appetite fragrance composition was produced in the same manner as the method.
When the degree of appetite reduction of the appetite-reducing fragrance composition produced by the above method was tested, the results shown in Table 8 below were obtained.

  The conditions for carrying out the experiment as in Example 1 were the same. Examining the results, Tokachi, which was not included in Example 1, windbreaks, etc., strengthened the effects of licorice and calmed the heat of the body, the effect of reducing appetite was further increased, and ginger and By including the glaze extract, the effect of supplementing the body was also obtained. However, since the scent inherent to herbal medicines is too strong, there were side effects that caused the user to feel upset and even caused vomiting.

  The greatest feature of the present invention obtained by the above extraction method is that each of the fragrance compositions is composed of a natural herbal material, and has an excellent deodorizing effect. The present inventor uses an fragrance composition composed only of herbal medicines, which is not a synthetic fragrance used in a conventional fragrance composition or a combination fragrance in which a synthetic fragrance and a natural oil are combined. A new fragrance composition with excellent deodorizing effects such as removal of malodors and tobacco odors, aiming to achieve a sense of stability and sedation by relaxing the mind and body while achieving the objectives I got a thing.

  In addition, most of the herbal medicines constituting the composition of the present invention remove moisture while suppressing heat in the primary dominance. From a Chinese medical perspective, most obese patients have many diseases caused by heat and humidity, so eliminating wet heat will solve the problems of obese patients.

Hereinafter, the function of the fragrance essential oil composition for diet extracted according to the present invention will be described through experimental examples.
[Experimental example]
1. Subjects of the experiment The animals subjected to this experiment were 5-week-old (25 g ± 2) ICR rats, which were obtained from a nationally recognized animal handler (Orient Bio Co, Kyung-Gi, Korea). The experimental animals were allowed to undergo an environmental adaptation period of 3 days, and then by random sampling, a control group (n = 8), a fasting group (n = 8), a fasting-diet fragrance essential oil composition scented inhalation group It was divided into three groups (n = 8). Fasted rats fed water only during 48 hours and did not feed. Rats are placed in a breeding cage (30 cm x 20 cm) for each group and maintained in the same laboratory environment where constant temperature (20 ± 2 ° C) and constant humidity (60%) are maintained, and the day and night are changed every 12 hours. did.
For inhalation of the fragrance essential oil composition scent for dieting, the fragrance essential oil composition is placed in a sealed plastic box (35 cm x 23 cm x 24 cm) in advance one day before the start of the experiment, and the scent is sufficiently contained in the plastic box. To spread.
The inhalation of the scent was carried out by a method in which the rats of the essential oil composition scent inhalation group were placed in a sealed box and repeatedly inhaled twice a day (10 am and 6 pm) for 1 hour. .

2. Measurement of memory capacity The step-down aviation task (NeuroLab, Korea) was used as a memory capacity measurement equipment. First, an experimental animal was stabilized for 2 minutes in a 7 × 25 cm flat foam (height 2.5 cm), and then the memory was recorded. A capacity measurement was performed. Recordings were taken when all four legs were delivered to a 1 cm spaced stainless steel rod (42 × 25 cm) placed in parallel by a rat placed on a flat foam. The rats that descended from the flat foam were given a 0.2 mA electrical stimulus for 2 seconds to cause the rats to memorize and then tested 1 hour later. The recording was measured according to the same procedure as described above, and no electrical stimulation was given. The time when all four legs arrived on the flat form was recorded up to 180 seconds.

3. Tissue Processing After the experiment, the animal was anesthetized by injecting Zoletile 50 (registered trademark R) (Vibac, Carros, France) into the peritoneal cavity of the experimental animal at 10 mg / kg, and then opened the chest cavity and passed through the left ventricle. 50 mM phosphate buffer saline (PBS) was injected for 5 minutes. Subsequently, 4% paraformaldehyde (PFA) fixative solution was perfused in 100 mM phosphate buffer (PB) for 10 minutes, and then the brain was removed and precipitated in 4% PFA solution at 4 ° C. for 24 hours. After fixing. Fixed brain tissue was precipitated in a 30% sucrose solution for one week, and serial transverse sections were prepared at a thickness of 40 μm using a freezing microtome (Leica, Nussloch, Germany).

4). Immunohistochemistry 1) Leptin immunohistochemistry Select brain sections and first inactivate hydrogen peroxide (H2O2) diluted in 1% 50 mM PBS to inactivate endogenous peroxidase present in the tissue. After reacting for 30 minutes, the cells were washed 3 times with 50 mM PBS, reacted with 1% bovine serum albumin (BSA) and 10% goat serum for 1 hour, and then rabbit anti-leptin antibody (1: 500, DiaSorni, Stillwater, MN, USA) and a primary antibody solution containing 0.05% BSA and 0.3% Triton X-100 for 12 hours at room temperature. Then, after reacting in a biotinylated rabbit secondary antibody (1: 2000, Vector Laboratories, Burlingame, CA, USA) for 1 hour, an avidin-biotin-peroxidase complex (Vectorstein ABC (trademark R) kit, Vector Laboratories) The reaction was allowed to proceed for 1 hour at room temperature. As the color former, 3,3′-diaminobenzidine tetrahydrochloride (DAB) was diluted to 0.02% in 0.05M Tris-HCl (pH 7.6), and 0.03% H 2 O 2 was added. For 5 minutes. The developed tissue was placed on a glass slide coated with gelatin and dried at room temperature for 2 hours, and then dehydrated while increasing the ethanol concentration to 70%, 80%, 90%, and 100%. Then, it was made transparent and sealed with polymount.

2) NPY immunohistochemistry A brain section is selected and first reacted with hydrogen peroxide (H2O2) diluted with 1% 50 mM PBS for 30 minutes to inactivate endogenous peroxidase present in the tissue. After washing with 50 mM PBS three times and reacting with 1% bovine serum albumin (BSA) and 10% goat serum for 1 hour, rabbit anti-leptin antibody (1: 500, DiaSorni, Stillwater, MN, USA) And a primary antibody solution containing 0.05% BSA and 0.3% Triton X-100 for 12 hours at room temperature. Then, after reacting in a biotinylated rabbit secondary antibody (1: 2000, Vector laboratories, Burlingame, CA, USA) for 1 hour, an avidin-biotin-peroxidase complex (Vectorstein ABC (trademark R) kit, Vector Laboratories) The reaction was allowed to proceed for 1 hour at room temperature. As the color former, 3,3′-diaminobenzidine tetrahydrochloride (DAB) was diluted to 0.02% in 0.05M Tris-HCl (pH 7.6), and 0.03% H 2 O 2 was added. For 5 minutes. The developed tissue was placed on a glass slide coated with gelatin and dried at room temperature for 2 hours, and then dehydrated while increasing the ethanol concentration to 70%, 80%, 90%, and 100%. Then, it was made transparent and sealed with polymount.

5. Measurement of BDNF protein expression To analyze hippocampal BDNF protein expression, the tissue was washed with PBS, then 50 mM HEPES (pH 7.5), 150 mM NaCl, 10% glycerol, 1% Triton X-100, 1.5 mM magnesium chloride hexahydrate, 1 mM ethylene glycol bis-2-aminoethyl ether tetraacetic acid (EGTA), 1 mM phenylmethylsulfonyl fluoride (PMSF), 2 μg / ml leupeptin, 1 μg / ml pepstatin After dissolving in a lysis buffer containing 1 mM sodium orthovanadate and 100 mM sodium fluoride, the protein was extracted, centrifuged and the supernatant was taken to quantify the protein. The protein thus extracted was electrophoresed on an SDS-polyacrylamide gel, and then transferred with a nitrocellulose membrane (Schleicher & Schuell GmbH, Dassel, Germany). After blocking the membrane thus transferred, the membrane was reacted with mouse actin antibody (1: 1000; Santa Cruz Biotech, Santa Cru, CA, USA) and rabbit BDNF antibody (1: 1000; Santa Cruz Biotech). Horseradish peroxidase-labeled rabbit anti-mouse antibody (1: 2000; Amersham Pharmacia Biotech GmbH, Freiburg, Germany) was used as the secondary antibody for actin, and anti-rabbit antibody (1: 4000; Santa Cruz biotech) was used as the secondary antibody for BDNF. Using. The results by Western blotting were confirmed by an enhanced chemiluminescence (ECL) detection system (Amersham Pharmacia Biotech GmbH).

6). Material Processing Method The material obtained by this experiment was calculated using the SPSS version 11.0 statistical program to calculate the mean ± standard error (standard error mean; SEM) for each item. Differences between populations for leptin, NPY, latency time and BDNF expression were post-tested by Duncan's method using a one-way analysis of variance (ANOVA). Significance level was set at P <0.05.

7). Experimental Results 1) Change in leptin positive cell expression The number of leptin positive cells showing appetite suppression in the hypothalamus of each group was 137.23 ± 10.12 in the control group (A), as shown in FIG. In B), it was 72.17 ± 5.22, and in the inhalation group (C) of the fragrance essential oil composition scent for fasting-diet, it was 111 ± 8.41.
As a result of this experiment, the number of leptin-positive cells in the fasted group was statistically significantly reduced compared to the control group (P <0.05), whereas the fragrance essential oil for fasting and dieting. The number of leptin positive cells was significantly increased in the inhalation group of the composition scent compared to the fasting group (P <0.05). For this reason, as a result of this experiment, the leptin expression indicating suppression of appetite was reduced by fasting, the appetite was increased, the inhalation of the fragrance essential oil composition for diet increased leptin expression, and the inhalation of the scent was caused by fasting It has been found to reduce increased appetite.

2) Changes in NPY positive cell expression Referring to FIG. 2, the number of NPY positive cells showing increased appetite in the hypothalamus of each group was 94.7 ± 4.12 in the control group (A), and the fasting group (B) 116.25 ± 1.90 in the fasting-diet fragrance essential oil composition fragrance inhalation group (C), 106 ± 3.15.
As a result of this experiment, the number of leptin-positive cells in the fasting group was statistically significantly increased compared to the control group (P <0.05), whereas the fragrance essential oil for fasting and dieting. The number of leptin positive cells was significantly reduced in the inhalation group of the composition scent compared to the fasting group (P <0.05). Therefore, as a result of this experiment, the leptin expression indicating appetite suppression was increased by fasting, the appetite was increased, the leptin expression was decreased by inhalation of the fragrance essential oil composition for diet, and the inhalation of the aroma was caused by fasting It has been found to reduce increased appetite.

3) Effect of inhalation of fragrance essential oil composition scent for diet on short-term memory ability In this experiment, in order to evaluate the effect of inhalation of fragrance essential oil composition scent for diet on short-term memory ability, Evasive behavior test. As a result of this experiment, the latency time was 84.0 ± 8.4 for the control group (A) and 32.0 ± 4.2 for the fasting group (B) as shown in FIG. The inhalation group (C) of the fragrance essential oil composition scent for diet was 60.9 ± 3.2.
As a result of this experiment, it was found that the short-term memory ability of the fasting group was significantly reduced compared to the control group (P <0.05), whereas the fragrance essential oil composition fragrance for fasting-diet The inhalation group was found to have significantly increased short-term memory ability compared to the fasting group (P <0.05). Therefore, as a result of this experiment, it was found that short-term memory ability was reduced by fasting, and inhalation of a fragrance essential oil composition aroma for diet improved short-term memory ability reduced by fasting.

4) Changes in BDNF protein expression Nerve growth factor BDNF protein expression in the hippocampal region was quantified by Western blotting. Referring to FIG. 4, when the expression of BDNF in the control group (A) is 1.00, the fasting group (B) is 0.71 ± 0.02, and the fragrance essential oil composition aroma for fasting-diet The inhalation group (B) was 0.85 ± 0.02.
As a result of this experiment, it was found that the BDNF protein expression was significantly decreased in the fasted group compared to the control group (P <0.05). On the other hand, it was found that the BDNF protein expression was significantly increased in the inhalation group of the fragrance essential oil composition aroma for fasting-diet compared with the fasting group (P <0.05). Therefore, as a result of this experiment, it was found that BDNF protein expression, which is a nerve growth factor, was decreased by fasting, and fasting suppressed the generation of nerves, and inhalation of the fragrance essential oil composition scent for diet was reduced by fasting. It can be seen that inhalation of the fragrance essential oil composition scent for dieting further increases nerve growth by increasing BDNF protein expression.

  The appetite-reducing fragrance composition according to the present invention provides an effect of weight loss while maintaining health while exerting the effect of suppressing the appetite of the natural herbal medicine of Fufutsu Seisanhara soaking into the human body. To do.

  In addition, the present invention provides an effect that greatly improves the health of the human body due to the scent of the herbal medicine, by using the herbal medicine that makes the human body healthy as a constituent component.

  Furthermore, the appetite-reducing fragrance composition according to the present invention provides the user with a stable scent and provides the effect that the emitted scent drifts around the place where the fragrance is provided for a long time.

  Furthermore, the reduced appetite fragrance composition according to the present invention does not cause spoilage due to various microorganisms even when used over a long period of time, and maintains the drying property of the decreased appetite fragrance composition for a long time. Provides the effect of preventing corruption.

Claims (3)

Ethanol, licorice extract, a bellflower extract, rhubarb extract, peppermint extract, Rhizoma Atractylodis Macrocephalae extract, Sen Qiu extract, including anorexia fragrance composition荊芥extract,
Licorice extract 0.5-1 part by weight, bellflower extract 0.5-1 part by weight, Daio yellow extract 0.3-0.7 part by weight, and light load 0.3-0.7 part by weight with respect to 90 parts by weight of ethanol Part, 0.5 to 1 part by weight of white birch extract, 0.3 to 0.7 part by weight of Senkyu extract, 0.3 to 0.7 part by weight of persimmon extract
An appetite-reducing fragrance composition characterized by the above .   The appetite-reducing fragrance composition according to claim 1, further comprising toki extract, windproof extract, eggplant extract, eel extract, ginger extract, ream extract, and birch extract. 0.3 to 0.7 parts by weight of Toki extract, 0.3 to 0.7 parts by weight of windproof extract, 0.3 to 0.7 parts by weight of coconut extract, and 0. 5 to 1 parts by weight, ginger extract 0.3 to 0.7 parts by weight, continuous extract 0.3 to 0.7 parts by weight, white ginger extract 0.3 to 0.7 parts by weight, The appetite-reducing fragrance composition according to claim 2 .

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