JP5565780B2 - Krycula 繭 pigment and its use - Google Patents

Description

  The present invention relates to a yellow pigment extracted from cricula cocoon (hereinafter sometimes abbreviated as “cricula cocoon pigment”) and uses thereof.

  In Indonesia, a large amount of black purple-colored worm-like cricula (Criculatrifenestrata) is produced every year around March. This crikula is a kind of wild boar, and its larvae eat and eat the leaves of street trees and so on. However, the cocoon produced when this samurai becomes a cocoon has a beautiful golden color, so it has been attracting attention as a “golden cocoon” in Japan, and various studies have been conducted in recent years.

  For example, in Japanese Patent Application Laid-Open No. 09-328000 (Patent Document 1), a crucible is used to form a decorative fabric by processing it into a sheet shape and forming it on a surface of a base sheet. It has been proposed to adhere.

  JP-A-2005-255673 (Patent Document 2) discloses that when curicula koji is extracted with a neutral aqueous liquid such as water alone or ethanol water at room temperature or by heating, the extract contains the most ultraviolet absorbing component. Extraction under pressure with a neutral aqueous solution in the range of 0.11 MPa to 0.4 MPa can be refined and defibrated to silk retaining a golden color, the extract has an ultraviolet absorption ability of 400 nm or less and a bacteriostatic effect, etc. Is described.

  In addition, JP 2006-70404 A (Patent Document 3) does not lose the unique form and color of crucible wrinkles, and also has gas adsorption properties, moisture absorption / release properties, UV cut, antibacterial properties, etc. As a method of manufacturing a sheet containing a piece having a design and functionality that makes the most of its characteristics, a cutting process that cuts a piece into a small sheet to form a piece, and an organic fiber containing the piece and silk in water There has been proposed a method comprising a slurry preparation step for dispersing into a slurry, and a paper making step for producing a sheet in which the slurry obtained in the slurry preparation step is made to embed the shards in organic fibers. The golden sheet by this method was used on the outer wall of the pavilion “Chubu Centennial Symbiosis Village” (the present inventor was engaged as a supervisor of the biological power of this pavilion) at the Aichi Expo, and has just attracted attention.

JP 09-328000 A JP 2005-255673 A JP 2006-70404 A

  However, at present, there is no knowledge about the pigment itself contained in the cricula camellia.

  An object of this invention is to extract the active ingredient of the pigment | dye contained in cricula koji, and to provide the utilization method.

As a result of diligent research to achieve the object described in the preceding paragraph, the present inventor contains a large amount of lutein, which is considered to be effective for cataract, which is a factor of eye disease, in the pigment contained in the extract described above. cage, there are UV-protective effect by this, that there is antioxidant in addition, more that the dye is excellent in color development of yellow, especially Jericho with the dye, Saku silkworm, wild silkworm, Tasaru silkworm It has been found that dyeing yarn taken from wild cocoons such as silkworms has excellent dyeability and fastness to light after dyeing, and this dye can be extracted efficiently by selecting the extraction conditions. Based on such knowledge, the present invention has been completed.

That is, the present invention relates to a lutein-containing yellow pigment characterized by being extracted from cricula koji.
The present invention also relates to a yellow dye or colorant containing the yellow pigment as an active ingredient.
And this invention concerns on the ultraviolet-ray shielding agent or antioxidant which uses said yellow pigment | dye as an active ingredient.
Furthermore, the present invention relates to a lutein-containing dietary supplement characterized by using the above yellow pigment as an active ingredient.

Such yellow pigment is preferably put in a pressure vessel such as an autoclave, and extracted by heating and drying in water at a temperature of 100 to 140 ° C. for 10 to 80 minutes, and then dried by freeze drying. Therefore, it can be efficiently obtained as a yellow pigment powder containing a large amount of lutein .

Powder Kurikyura cocoon dyes according to the invention, dyeing fee (stain) and colorants as such rather than Rubakari excellent because it has a UV resistance and antioxidant activity, UV agents or antioxidants to prevent the harmful ultraviolet Become an agent. Therefore, the fabrics dyed with the cricule wrinkle dye and the cosmetics containing the cricula wrinkle dye have an excellent ultraviolet blocking effect. In addition, this curicula cocoon pigment powder is also useful as a dietary supplement because of its extremely high lutein content.

This is an analysis chart of the components of clicula pigments measured by high performance liquid chromatography. The graph which shows the ultraviolet-ray cut effect | action of a curicula cocoon pigment powder and a rabbit's cocoon.

  Hereinafter, embodiments of the present invention will be described in detail.

  Cricula wrinkles have a special structure in which the fibers have an ultraviolet ray-cutting effect, and the color is used as a golden crucible wrinkle itself. That is, the crucible candy having such characteristics can be used as a substitute for mere cloth or paper, such as packaging materials, craft materials, interior materials, etc. Can be used for anything that plays a role. In addition to exhibiting a decorative effect, such a material has an ultraviolet ray blocking action, so that it is difficult to fade.

  The present invention does not use cricula cocoon as it is, but uses pigment extracted from the cocoon. Here, a method for extracting and producing a pigment from cricula koji will be described. Extraction of pigments is applied to the extraction process with extractant (extraction solvent) such as water after removing the cocoon from the cocoon, or the cocoon from which the cocoon has been removed or the cocoon with the cocoon removed. Can be done.

As a condition for water extraction of the crucible soot pigment, any conditions can be adopted as long as the dye can be eluted from the soot into the extraction water, but from the viewpoint of extraction efficiency and quality, the following conditions are used. Is preferably employed.
First, the amount of water used for extraction is usually 1 to 5 liters per 100 g of straw. If the amount of water is too small relative to the koji, extraction will be insufficient. On the other hand, if the amount is too large, a large amount of diluted extract will be generated, resulting in the extra time and energy required for concentration of the extract.

  The extraction temperature is, for example, 60 to 200 ° C., preferably 100 to 140 ° C. in a pressure vessel such as an autoclave. If this temperature is too low, the progress of dye elution will be extremely slow, while if it is too high, sericin will elute and further hydrolysis of sericin will occur. The extraction time is 5 to 80 minutes, preferably 5 to 20 minutes. If this time is insufficient, the extraction is not sufficiently performed, and it is useless to continue the extraction operation after the extraction is completed. In general, in the combination within these temperature ranges and time ranges, the dye extraction is completed in a short time when the temperature is high, and in a long time when the temperature is low.

  The dye extract obtained by the above method can be cooled, filtered if necessary, and used as it is for staining etc. in the present invention, but in the present invention, it is dried by a method such as freeze drying. It is preferable to use a powder form since the storage and handling properties are improved. In addition, the dried powdered cricula koji color is very convenient when used as a dye (dyeing agent) because the concentration of the dyeing solution can be easily adjusted. Thus, the powdering of the crucible soot pigment has various merits as described above. However, the powder of the crucible soot pigment has not been known at all so far and has been realized for the first time by the present invention.

  As a result of the inventor's research, it has been found that the components of the cricula cocoon pigment powder extracted and produced as described above contain a large amount of lutein as described above. This active ingredient has an ultraviolet blocking action and an antioxidant action. It can also be used as a dietary supplement.

Next, the use of the crucible soot pigment extracted and produced in this way will be described.
Cricula cocoon pigment can be used as a dye as a dye, as well as the color of yarn taken from other cocoon cocoons dyed with clicula cocoon pigment or the fabric woven from it is taken from clicula cocoon It has a bright and golden color compared to the yellow color of the yarn or the fabric woven with it, giving yellow an excellent color, and light fastness comparable to that of plant dyeing (see Example 3 below). This is because the cricula cocoon yarn is short and stiff, so it is steamed more than necessary when twisted, so that the pigment of the sericin layer on the surface drops, and the surface structure is broken, causing discoloration. However, when the cruciferous cocoon pigment is taken out and dyed by the above-described method, the golden color of the cricula moth is beautifully reproduced in the fabric woven with yarns from other moths, in particular, wild moths. In addition, cricula wrinkle pigment can be used as a coloring agent for cosmetics, foods and the like.

Lutein, an active ingredient contained in a large amount in Curicula sputum, is a kind of carotenoid pigment contained in the cornea of the eye and is said to be effective for cataract, which is a cause of eye disease. It can be applied as a dietary supplement. By the way, other lutein extracts include marigold, avocado, spinach, etc. For example, it was found that the extracted cricula pigment contains 60 to 130 times more lutein than the same amount of spinach ( See Examples below). Such active ingredients from secretions of animals example extracted has not been reported so far, the case of Kurikyura, ingested active ingredients by being concentrated in the body, it contains a large amount of lutein in cocoon believed to have, the present invention is Ru can also be referred to as novel lutein extraction method.

  For example, it can be used as a raw material for UV-cutting cosmetics, UV-cutting clothing, UV-cutting agents such as UV-cutting parasols. By the way, when comparing the silk fabric of the rabbit itself with the silk fabric dyed with the crucible silk pigment, the latter cuts ultraviolet rays in a wider range of wavelengths (see Example 3 below). In addition, side effects such as allergies do not occur in cricula vaginal pigment, which is a feature not seen in chemical stains.

  Cricula cocoon pigment can be used as a food coloring material, as well as it can be used as a food material as an antioxidant because it has an antioxidant action as described above (see below) (See Example 4).

  Hereinafter, the present invention will be described in more detail with reference to examples. However, the present invention is not limited to these examples. In the examples, “%” means “% by weight” unless otherwise specified.

[Example 1]
(Manufacture of Kuricula 繭 pigment powder)
100 g of cricula cake from which wrinkles were removed and 3 liters of water were placed in an autoclave and heat-treated at 120 ° C. for 10 minutes to obtain a dye extract. This was cooled to room temperature, and the filtrate obtained after filtration was freeze-dried to obtain 1-2 g of yellow pigment powder.

(Analysis of ingredients containing cricula cocoon pigment powder)
The cricula pigment powder produced in the same manner as in Example 1 was measured by high performance liquid chromatography (HPLC). The measurement conditions of this high performance liquid chromatography are as follows.
Model: LC-10AS (Shimadzu Corporation)
Detector: UV-visible spectrophotometer SPD-10AV (Shimadzu Corporation)
Column: Shimadu CLC-SIL (M), φ4.6 mm × 150 mm
Mobile phase: hexane and acetone suspension (81:19)
Flow rate: 1.1 ml / min
Wavelength: 450nm
Temperature: 30 ° C

  The results are shown in FIG. The peak at 7.817 in FIG. 1 is lutein, the peak at 8.245 is zeaxanthin, and the peaks at 9.797, 10.1440, and 10.814 are the other lutein isomers. As can be seen from FIG. 1, it is found that the clicula pigment powder of the present invention contains 66.9% lutein, 4.2% zeaxanthin, and 19.8% other lutein isomers. It was.

[Example 2]
In this example, in order to examine the UV-cutting effect of cricula wrinkle pigment, silkworm silk of silkworms (Japanese Standards Association, with 14 silks) was prepared in a conventional manner using cricula wrinkle pigment powder produced in the same manner as in Example 1. Stained. The ultraviolet spectrum of the dyed silk fabric and the unstained silk fabric was measured for the absorption spectrum from 220 to 800 nm using a spectrophotometer “Hitachi U-4000” equipped with an integrating sphere analyzer. .

The results are shown in FIG. In FIG. 2, curve A shows the ultraviolet absorption curve of silk fabric of rabbits dyed with cricula silk pigment, and curve B shows the ultraviolet absorption curve of silk fabric of unstained rabbits.
As can be seen from FIG. 2, the silk fabric of the rabbit itself has a UVB (wavelength of 280 to 315 nm), which is said to cause ultraviolet rays, as compared with the fabric dyed with the cricula silk pigment. UVA (wavelength of 315 to 400 nm), which is said to be, does not cut so much (that is, does not absorb), whereas silk fabric dyed with cricula cocoon pigment cuts both UVB and UVA significantly.

[Example 3]
In this example, in order to examine the light fastness of the cricula wrinkle pigment, silkworm silk of silkworms (Japanese Standards Association, with 14 meshes) is dyed by a conventional method using the cricula wrinkle pigment produced in the same manner as in Example 1. did.

The silk fabric dyed in this manner was subjected to ultraviolet long life feed meter (Suga Test Instruments Co., Ltd. UV Long Life Fade Meter).
U48) was irradiated with ultraviolet rays for 7 hours and 30 hours at 20 ° C. and humidity of 60%, compared with the JIS standard blue scale, the Lab value was measured, the color difference was determined, and the grade was determined.

The color difference due to ultraviolet irradiation was determined from Hunter's color difference formula. Hunter's color difference equation is a method for obtaining a color difference (ΔE) by setting the Lab values before ultraviolet irradiation as L1, a1, b1, and the values after ultraviolet irradiation as L2, a2, b2, and was obtained from the following equations.

As a result, while a Delta] E = 3.70 in ΔE = 9.72,30 hours irradiation at fastness tertiary seven hours irradiation, Delta] E = 2 stained with fabric 7 hours irradiation at Kurikyura cocoon dye. At 04 and 30 hours of irradiation, ΔE = 3.34 and the fastness is third grade. This is as fast as the dyes of vegetative dyes.

[Example 4]
In this example, in order to examine the antioxidant effect of cricula sputum pigment, cricula sputum was treated with hot water in the same manner as in Example 1 to obtain a pigment extract. The antioxidant ability of this dye extract was measured by the electron spin resonance (ESR) method. As a result, the detection limit of 30 Unit / g by this measurement method was 60 Unit / g in the extract of Cricura sputum, and it was confirmed that the antioxidant property was excellent.

Kurikyura cocoon dyes of the invention, dye charge (stain) or colorant and to lutein-containing dietary supplements by blending it in addition to use, UV effects or antioxidant power of certain cosmetics and UV clothing and umbrellas, etc. Ru is easily provided. That is, UV action by Kurikyura dyes, if utilized light fastness, as described above, UV cosmetics and can provide clothing and umbrellas such as fashionable ultraviolet cut shirt.

Claims (6)

A yellow pigment characterized by containing lutein , zeaxanthin and a lutein isomer, which is a pigment extracted from cricula koji at 100 to 140 ° C with hot water .   The yellow pigment according to claim 1, wherein the pigment is in a powder form. The yellow pigment | dye of Claim 1 or 2 whose content of lutein in the said pigment | dye is 66% or more. A yellow dye comprising the yellow pigment according to any one of claims 1 to 3 as an active ingredient. A colorant comprising the yellow pigment according to any one of claims 1 to 3 as an active ingredient. An ultraviolet blocking agent comprising the yellow pigment according to any one of claims 1 to 3 as an active ingredient.