JP5521211B2 - Flow cell - Google Patents

Description

  The present invention relates to a sealing material excellent in chemical resistance, heat resistance and liquid tightness, and a flow cell including the same. In particular, the flow cell of the present invention is useful as a flow cell used for a differential refractometer, and is further useful as a flow cell used for a differential refractometer for molecular exclusion chromatography at high temperatures.

  As a substance dissolves in a solvent, the refractive index of the solvent generally changes. Therefore, a differential refractometer that measures the difference in refractive index between a solvent (referred to as a reference solution) and a solvent in which the component is dissolved (referred to as a sample solution) is used as a general-purpose detector for components eluted from a column in a liquid chromatograph. Is used. As a differential refractometer, a Fresnel type differential refractometer that detects a change in reflected light intensity due to a refractive index and a deflection type (also referred to as a Bryce type) differential refractometer that detects a change in refraction angle are known.

  In a normally used deflection type differential refractometer, parallel light generated from a light source is introduced into a flow cell, and the traveling direction of the parallel light is deflected according to the refractive index of the reference liquid and sample liquid passing through the flow cell. The degree of deflection is detected by a position detection light sensor. The flow cell is provided with a flow path having two right-angled triangular sections partitioned by a swash plate inclined with respect to the light traveling direction (optical axis) inside a transparent body such as quartz glass, and the two flow paths The parallel light is transmitted through the sample liquid and the reference liquid respectively. From the magnitude of the change in angle (deflection) in the traveling direction of the transmitted parallel light, the sample concentration in the sample solution that causes the difference in refractive index can be obtained. FIG. 1 shows the internal structure of the flow cell 10. A sample liquid side flow path 11 composed of a triangular column-shaped liquid flow path for circulating the sample liquid and a reference liquid side flow path 12 composed of a triangular column-shaped liquid flow path for flowing the reference liquid are partitioned by a transparent swash plate 13. ing. The inlet 16 of the sample liquid side channel is connected to the pipe from the analytical column, and the outlet 17 is connected to the pipe to the waste liquid line. The inlet 18 of the reference liquid side channel is connected to a pipe to which an eluent is supplied, and the outlet 19 is connected to a pipe to a waste liquid line.

  High temperature molecular exclusion chromatography (hereinafter abbreviated as high temperature SEC) is used for analysis of polyolefins such as polyethylene and polypropylene which are not soluble at room temperature and engineering plastics, and can analyze up to 180 ° C. In addition, an ultra-high temperature GPC that analyzes super engineering plastics such as polyether ether ketone, polyimide, polyether imide, and fluororesin at a high temperature up to about 250 ° C. has been developed. High temperature SEC is often referred to as high temperature GPC meaning high temperature gel permeation chromatography. In this specification, high temperature SEC and high temperature GPC are used interchangeably.

  The flow cell of the differential refractometer used in high temperature GPC usually uses quartz glass having excellent optical transparency, heat resistance and pressure resistance as a material. In addition, it is preferable to protect the periphery of the flow cell with a metal housing because the optical positioning of the flow cell can be secured, the flow cell pressure resistance can be increased, and the flow cell can be maintained under high temperature conditions. The housing is also referred to as a piping block because it is used for connection between the flow cell and external piping, and is also referred to as a cell block because it protects the cell.

  It is necessary to insert a sealing material such as a gasket (also called packing) or an O-ring at the inlet / outlet provided on the flow cell and the connection between the outlet and the piping block in order to prevent liquid leakage (to make liquid-tight connection). There is. As a sealing material used for the flow cell of the differential refractometer for high temperature GPC, polytetrafluoroethylene having high chemical resistance and perfluoroelastomer having excellent heat resistance such as Kalrez (registered trademark) are used ( Non-patent document 1).

  Fluoropolymers are widely used in various synthetic chemical research and various fields due to their excellent chemical resistance, heat resistance, weather resistance, flame resistance, low friction, non-adhesiveness, electrical properties, biocompatibility, etc. Applied research is being conducted. In synthetic chemical research, fluorine organic solvents that dissolve fluorine polymers such as hydrochlorofluorocarbons, hydrofluorocarbons, hydrofluorobenzenes, hydrofluoroketones, hydrofluoroalkylbenzenes, hydrofluoroethers. And hydrofluoroalcohols are used, and high temperature GPC or ultra high temperature GPC is required for analysis of the synthesized polymer. However, the seal material made of polytetrafluoroethylene or perfluoroelastomer used for the differential refractometer flow cell such as high temperature GPC is swollen and deteriorated with respect to the above-mentioned fluorine-based organic solvent under high temperature and high pressure. There was a risk that the sealability could be affected by deformation.

  As another example of the sealing material used for the connection between the cell and the piping block, between the metal cell body of the spectroscopic measurement cell used for gas component analysis and the glass window material attached to the tip of the cell. The sealing material inserted in is disclosed (patent document 1). The sealing material is composed of a coil spring-like annular material that can be elastically deformed in the thickness direction, and a sheath (sheath) having a C-shaped cross section made of a corrosion-resistant metal that covers the annular material. However, it is possible to manufacture a sealing material having a structure using such a coil spring as small as an inner diameter of about 2 mm or less that can be used for a liquid contact part of a flow cell of a differential refractometer, or to make a liquid-tight liquid of a high pressure state. It is doubtful whether sex can be secured.

Japanese Patent Laid-Open No. 9-311104

Uematsubara Hajime, Sato Tatsushi; Tosoh Research Report, 42, 3-16 (1998)

  An object of the present invention is to provide a sealing material useful for liquid-tightly connecting flow paths through which a solvent such as a fluorine-based organic solvent flows, and a flow cell including the sealing material.

  The inventor has improved chemical resistance, heat resistance and flexibility against solvents such as fluorine-based organic solvents that cause swelling, deterioration, deformation, etc. to polytetrafluoroethylene and perfluoroelastomers that have been used as conventional sealing materials. As a result of searching for the sealing material provided, the present invention was completed.

  That is, the first invention is a sealing material for liquid-tightly connecting flow paths made of gold.

The second invention
It has two hollow parts for allowing the reference liquid and the sample liquid, which are partitioned by a swash plate inclined with respect to the optical axis, to pass through the reference liquid or the sample liquid in a direction perpendicular to the optical axis. A rectangular parallelepiped cell provided with an inlet and an outlet
Piping connected to the inlet and outlet provided in the cell;
A sealing material made of gold for connecting the inlet and outlet provided in the cell and the pipe in a liquid-tight manner; and
It is a flow cell provided with.

  Moreover, 3rd invention is described in 2nd invention whose length of the inflow port provided in the cell and the outflow port is 35 to 45% of the length between the side surfaces which provided the inflow port and the outflow port. It is a flow cell.

  According to a fourth aspect of the present invention, the axis of the inlet and outlet for passing the sample liquid provided in the cell coincides with the axis of the inlet and outlet for allowing the reference liquid to pass through. A flow cell according to the second or third invention.

  According to a fifth aspect of the present invention, there is provided a housing according to the second to fourth aspects, further comprising a housing provided with an inflow port for allowing the sample liquid and the reference liquid to pass therethrough and an internal flow path for connecting the outlet and the pipe. The flow cell according to any one of the above.

  Hereinafter, the present invention will be described in detail.

  The present invention is characterized in that the material of the sealing material for liquid-tightly connecting the flow paths is made of gold. Gold (Au) is the most stable metal and does not oxidize in air or water. Also, it does not oxidize or discolor when heated in air. Although gold reacts with aqua regia, hydrocyanic acid, chlorine, bromine, iodine potassium, etc., it is not affected by organic solvents including fluorinated organic solvents. Furthermore, gold is extremely flexible and has the best extensibility among all metals, so that it is excellent in workability, and it is easy to manufacture a sealing material having a fine shape such as a gasket or an O-ring. Therefore, it can be said that gold is preferable as a sealing material having chemical resistance, heat resistance and flexibility. In the present invention, the “seal material made of gold” is not limited to a seal material made of gold having a purity of 99.99% or more, that is, pure gold (24 gold), but has chemical resistance, heat resistance and flexibility. Even a sealing material made of an alloy in which a metal such as silver is contained in pure gold within a range that does not impair the properties, is included in the range of the “sealing material made of gold” in the present invention. The thickness and shape of the sealing material of the present invention are not limited, and may be appropriately designed according to the shape, diameter size, flow rate, liquid feeding pressure resistance, etc. of the fluid-tightly connected flow path.

As one aspect of the apparatus provided with the sealing material of the present invention,
It has two hollow parts for allowing the reference liquid and the sample liquid, which are partitioned by a swash plate inclined with respect to the optical axis, to pass through the reference liquid or the sample liquid in a direction perpendicular to the optical axis. A rectangular parallelepiped cell provided with an inlet and an outlet
Piping connected to the inlet and outlet provided in the cell;
A sealing material made of gold for connecting the inlet and outlet provided in the cell and the pipe in a liquid-tight manner; and
A flow cell equipped with The material of the cell constituting the flow cell of the present invention may be selected in consideration of light permeability and liquid corrosion resistance. In many cases, transparent quartz glass is used. Further, all or a part other than the part through which light passes can be made of an opaque material such as black quartz glass. Another aspect of the cell is a cell in which the cross-sectional area of the hollow part through which the sample liquid flows is smaller than the cross-sectional area of the hollow part through which the reference liquid flows in order to prevent the target component dissolved in the sample liquid from spreading.

  The material of the sealing material of the present invention is made of gold (pure gold or an alloy in which pure gold contains a metal such as silver as long as the properties of gold are not impaired). Therefore, in the flow cell of the present invention, when the cell and the pipe are connected in a liquid-tight manner using the sealing material of the present invention, a sealing material made of a resin (polytetrafluoroethylene, perfluoroelastomer, etc.) that has been used conventionally. It is necessary to increase the clamping pressure between the cell and the pipe as compared with the above. Therefore, in the general cell shown in FIG. 1, there is a possibility of destruction from the contact point with the sealing material due to the tightening pressure (see Example 1). One mode (plan sectional view) of a preferred cell 10 constituting the flow cell of the present invention is shown in FIG. The cell 10 in FIG. 2 is a quartz cell having a rectangular parallelepiped shape. The two hollow portions (sample solution side channel 11 and reference solution side channel 12) that constitute the cell 10 are parallel to each other through the swash plate 13, and the side surfaces that form a right angle of the triangle column are arranged. It is provided so as to be parallel or perpendicular to the side surface of the rectangular parallelepiped. The inlet to the hollow part (sample liquid side channel inlet 16 and reference liquid side channel inlet 18) and the outlet (sample liquid side channel outlet 17 and reference liquid side channel outlet 19) are hollow parts. Among the side surfaces of the triangular prism forming the right angle, the cell 10 is provided as a through-hole penetrating to the outside of the flow cell main body at a right angle from the side surface through which light does not pass. The length of the inlet and outlet (e1 and e2 in FIG. 2) is preferably 35% to 45% of the length between the side surfaces of the cell 10 where the inlet and outlet are provided (d in FIG. 2). . The reason for this is that if the length of the inlet and outlet is 35% or less of the length between the side surfaces where the inlet and outlet are provided, the cell and piping are liquid-tight via the sealing material (gasket) of the present invention. May cause damage to the cell when connected to the tube, and if the length of the inlet and outlet is 45% or more of the length between the sides where the inlet and outlet are provided, the sample peak in the chromatogram may spread Because there is. Further, when the length of the inlet and outlet is 45% or more of the length between the side surfaces provided with the inlet and outlet, the length between the side surfaces (side surfaces through which light does not pass) provided with the inlet and outlet ports. The length (d in FIG. 2) exceeds 10 times the length of the side that sandwiches the right angle of the hollow triangular prism (e3 in FIG. 2). As for the positions of the inlet and outlet, as in the cell 10 of FIG. 2, the axis of the direction in which the liquid flows in the sample liquid side channel inlet 16 and the sample liquid side channel outlet 17 and the sample liquid side It is preferable to provide the flow path inlet 18 and the sample liquid side flow path outlet 19 so that the axes in the direction in which the liquid flows coincide with each other. The reason for this is that if the axes of the inlet and outlet are deviated, a torsional moment acts on the cell, and a non-uniform force is applied to the tightening portion, which may damage the cell. In addition, it is preferable to provide the inflow ports 16 and 18 at the lower end of the triangular prism that constitutes the hollow portion and the outlets 17 and 19 at the upper end of the triangular prism that constitutes the hollow portion so that a portion where the flow is stagnant does not occur inside the cell. .

As a preferred embodiment of the flow cell of the present invention,
It has two hollow parts for allowing the reference liquid and the sample liquid, which are partitioned by a swash plate inclined with respect to the optical axis, to pass through the reference liquid or the sample liquid in a direction perpendicular to the optical axis. A rectangular parallelepiped cell provided with an inlet and an outlet
Piping connected to the inlet and outlet provided in the cell;
A sealing material made of gold for connecting the inlet and outlet provided in the cell and the pipe in a liquid-tight manner; and
A housing provided with an internal flow path for connecting the inlet and outlet for passing the sample liquid and the reference liquid and the piping;
A flow cell equipped with

The housing constituting the preferred flow cell secures optical positioning of the cell by protecting the periphery of the cell, improves the pressure resistance of the cell, maintains the cell temperature under high temperature conditions, and connects the cell and piping. Used. The housing may be protected so as to surround a portion of the cell other than the surface through which light passes.
A first housing member provided with an internal flow path for connecting the inlet and outlet for passing the sample liquid and the pipe;
A second housing member provided with an internal flow path for connecting the inlet and outlet for passing the reference liquid and the pipe;
Means for securing the first housing member and the second housing member via a cell;
And a housing provided with. The connection between the internal flow path and the pipe may be directly welded, fixed with a fastening means such as a screw, or fixed by applying a taper process for a known tube connector. May be. The material constituting the housing needs to be a metal with high thermal conductivity because it is necessary to keep the cell at a high temperature, and stainless steel is preferable because it needs to be contacted with various chemicals.

  The present invention is characterized in that the material of the sealing material for liquid-tightly connecting the flow paths is made of gold. Gold has chemical resistance and flexibility. Therefore, the flow paths should be connected fluid-tight without periodic replacement of the sealing material, even for solvents such as fluorinated organic solvents, which had to be replaced regularly with conventional resin sealing materials. Is possible. In addition, since gold also has heat resistance, it is particularly preferable as a sealing material for liquid-tightly connecting channels provided in a device used under high temperature and high pressure conditions such as high temperature GPC or ultra high temperature GPC.

It is an aspect of an apparatus provided with the sealing material of the present invention,
It has two hollow parts for allowing the reference liquid and the sample liquid, which are partitioned by a swash plate inclined with respect to the optical axis, to pass through the reference liquid or the sample liquid in a direction perpendicular to the optical axis. A rectangular parallelepiped cell provided with an inlet and an outlet
Piping connected to the inlet and outlet provided in the cell;
A sealing material made of gold for connecting the inlet and outlet provided in the cell and the pipe in a liquid-tight manner; and
As a mobile phase, the flow cell provided with is required to replace the sealing material periodically in the conventional flow cell, and even if a solvent such as a fluorine-based organic solvent is used, it is not necessary to replace the sealing material. Therefore, by providing the flow cell of the present invention in an analyzer used for a liquid chromatograph such as a differential refractometer, an analyzer that can be easily maintained and can be analyzed stably can be provided.

  Since the material of the sealing material of the present invention is gold, that is, metal, it is necessary to increase the clamping pressure when connecting the cell and the pipe in the flow cell of the present invention as compared with the conventional resin sealing material. Therefore, in the flow cell of the present invention, the length of the inlet and outlet provided in the cell is 35% to 45% of the length between the side surfaces provided with the inlet and outlet, and / or the sample liquid is Matching the inlet and outlet axes for passing the reference liquid with the inlet and outlet axes for passing the reference liquid reduces the risk of cell damage due to clamping pressure or sample / reference liquid inflow pressure Therefore, it is preferable.

  Furthermore, if the flow cell of the present invention further comprises a housing provided with an internal flow path for connecting the inlet and outlet and the pipe for allowing the sample liquid and the reference liquid to pass through, the pressure resistance of the cell is improved. Since the cell temperature can be maintained under a high temperature condition, it is particularly preferable as a flow cell provided in an analyzer used for analysis under high temperature and high pressure such as high temperature GPC or ultra-high temperature GPC.

The figure which shows the internal structure of the general flow cell (cell) used with a differential refractometer. The figure (plan sectional drawing) which shows the one aspect | mode of the preferable cell which comprises the flow cell of this invention. The figure which shows the one aspect | mode of the flow cell of this invention. The assembly block diagram of the one aspect | mode of the flow cell of this invention. The plane sectional view of the cell examined in the example. The figure which shows the pressurization simulation result of the cell A. The figure which shows the pressurization simulation result of the cell B. FIG. The figure which shows the pressurization simulation result of the cell C. FIG. The flow-path block diagram of the apparatus used in Example 2 (pressure-resistant leak test).

  One embodiment of the flow cell 100 of the present invention is shown in FIG. The cell 10 has the same structure as the cell 10 shown in FIG. The cell 10 is held by the housing 30 via four sealing materials (gaskets) 20 of the present invention at the inlets 16 and 18 and the outlets 17 and 19 provided in the sample liquid side channel and the reference liquid side channel. Has been. The inlet 16 of the sample liquid side channel is a pipe connection port 36 from the analytical column, the outlet 17 of the sample liquid side channel is a pipe connection port 37 to the waste liquid line, and the inlet 18 of the reference liquid side channel is The piping connection port 38 for supplying the eluent and the outlet 19 of the reference liquid side channel are connected to the piping connection port 39 to the waste liquid line, respectively. The housing 30 is composed of a pair of housing members 30a and 30b. While putting the gap gauge in the upper and lower tightening adjustment margin 35, the upper and lower bolts 40 are evenly arranged so that the tightening adjustment margin 35 is about 0.4 mm. By tightening, the cell 10 is held by the housing 30 via the sealing material (gasket) 20 of the present invention. FIG. 4 is an assembly configuration diagram of the flow cell 100 of the present invention. The housing 30 is provided with depressions 31 and 32 useful for positioning the sealing material (gasket) 20 of the present invention. In FIG. 4, since the recess 31 is a portion connected to a pipe connection port from the analysis column, the sample is prevented from spreading by making a hole provided in the recess 31 narrow. In addition, since the recess 32 is a portion connected to a pipe connection port to the waste liquid line, an excessive back pressure is not applied to the cell 10 by making a hole provided in the recess 32 thick.

  EXAMPLES Hereinafter, although an Example demonstrates this invention further in detail, this invention is not limited to an Example.

Example 1
Using three-dimensional model creation software SolidWorks 2007 (product name) and analysis software CosmosWorks 2007 (product name), the degree of displacement when a load / pressure was applied to the cell via a sealing material (gasket) was simulated.

  The material of the cell was synthetic quartz, the material of the sealing material (gasket) was pure gold, the pressure condition was 0.3 MPa, and the physical properties of each material were used for analysis. Three types of cells were examined. The cell A is a rectangular parallelepiped of d4.8 mm × f5 mm × height 12.8 mm, and the length in the d direction of the inlet and outlet 16, 17, 18, 19 (the length between the side surfaces where the inlet and outlet are provided) S) A cell in which (e1 and e2) is 1.4 mm (a plan sectional view is shown in FIG. 5A). The cell B is a cell having a rectangular parallelepiped of d10 mm × f5 mm × height 12.8 mm, and the lengths (e1 and e2) in the d direction of the inlets and outlets 16, 17, 18, and 19 are 4 mm (plane cross section) The figure is shown in FIG. 5 (b)). In the cell C, the axis of the inlet and outlet 16 and 17 of the sample liquid side channel and the axis of the inlet and outlet 18 and 19 of the reference liquid side channel are shifted by 1 mm in the cell B (g1) cell. (A plan sectional view is shown in FIG. 5C).

  FIG. 6 (a) shows a front cross-sectional view before applying a load / pressure to the cell A via the sealing material (gasket) 20 in FIG. 6 (a). The figure (simulation result) is shown in FIG. The cell A has a relatively thin wall at the portion where the flow path is provided, and when a tightening load is applied to the pure gold sealing material (gasket) 20 so that liquid leakage does not occur, the sealing material (gasket) 20 becomes the cell 10. The wall thickness of the part where the flow path is not provided (d = 4.8 mm) and the wall thickness of the part where the flow path is provided (same as the length in the d direction of the inlet and outlet, e1 = e2 = 1.4 mm) is large (1: 0.29), so that the distortion of the portion in contact with the sealing material (gasket) 20 becomes unbalanced. Therefore, it was anticipated that the hollow portion of the cell 10 may be greatly deformed, and the cell 10 may be broken from the inlet and outlet portions 16, 17, 18, and 19.

  FIG. 7 (a) shows a front cross-sectional view before applying a load / pressure to the cell B through the sealing material (gasket) 20 in FIG. 7 (a), and FIG. The figure (simulation result) is shown in FIG. In the cell B, the wall thickness (the length in the d direction of the inlet and outlet) of the portion where the flow path is provided is thicker than that of the cell A. Therefore, even if a tightening load is applied to the pure gold sealing material (gasket) 20 so as not to leak liquid, the thickness of the wall of the portion where the flow path is not provided (d = 10 mm) and the wall thickness of the portion where the flow path is provided (same as the length in the d direction of the inlet and outlet, e1 = e2 = 4 mm) is relatively small (1: 0.4), and the seal The distortion of the portion in contact with the material (gasket) 20 is balanced. Therefore, the hollow part of the cell 10 was hardly deformed, and it was expected that the cell 10 could sufficiently withstand the tightening of the sealing material (gasket) 20. In addition, the capacity of the inlet and outlet is increased by increasing the wall thickness of the portion where the flow path is provided (the length of the inlet and outlet in the d direction is increased). The cell 10 is not directly connected to the outlet, and actually does not have a great influence on the analysis as compared with the piping capacity from the analytical column to the cell 10.

  FIG. 8 (a) shows a front cross-sectional view (CC 'cross-sectional view of FIG. 5 (c)) before applying a load / pressure to the cell C through the sealing material (gasket) 20, and FIG. The figure (simulation result) is shown in FIG. In the cell C, the axes of the inlet and outlets 16 and 17 of the sample liquid side channel do not coincide with the axes of the inlet and outlets 18 and 19 of the reference liquid side channel. Therefore, when a load / pressure is applied to the cell 10 through the sealing material (gasket) 20, the swash plate 13 separates the inlet, the outlet, the sample liquid side channel and the reference liquid side channel as compared with the cell B. It was predicted that the cell 10 could be destroyed due to large deformation.

Example 2
The pressure leak test of the flow cell 100 of the present invention was performed using the apparatus shown in FIG. That is, the pure water put in the eluent container 200 is sucked up by the liquid feed pump 300, the flow path is branched through the pressure sensor 400, and the sample liquid side flow path and the reference liquid side flow path in the cell constituting the flow cell 100 are separated. Guide to the inlet. The pure water that has passed through the sample liquid side flow path and the reference liquid side flow path in the cells constituting the flow cell 100 and has exited from the respective outlets is merged and then sent to the waste liquid container 600 through the resistance tube 500. . The resistance tube 500 is introduced to give 0.5 MPa, which is the upper limit of the pressure resistance of the flow cell of the differential refractometer in the high-temperature GPC device, to the measurement system. The sealing material (gasket) of the present invention provided in the flow cell 100 is made of pure gold and has an annular shape with a rectangular cross section, and the dimensions are outer diameter φ2.3 mm × inner diameter φ1.35 mm × thickness 0.6 mm.

  Before connecting the flow cell 100 to the apparatus shown in FIG. 9, the flow rate was measured at a flow rate at which the display of the pressure sensor 400 was 0.5 MPa, and as a result, about 2 mL / min was sent. In the cell constituting the flow cell used in this example, the wall thickness of the portion provided with the flow path described in Example 1 is relatively thin (the length of the inlet and the outlet in the d direction is relatively small). (Short) Cell A (FIG. 5 (a)) and the wall thickness of the portion provided with the flow path are relatively thick (the inlet and outlet are relatively long in the d direction) Cell B (FIG. 5 ( b)). The flow cell 100 obtained by assembling the housing to the cell by the method shown in FIG. 4 is connected to the apparatus shown in FIG. 9, and is tightened with a bolt so that the pressure resistance of the flow cell becomes the target 0.5 MPa while feeding the liquid. The housing member constituting the flow cell was tightened.

  The cell A was broken in a state where the bolts were tightened and the liquid feeding pressure did not increase (indicated by 0 MPa). Destruction occurred near the inlet and outlet. On the other hand, in the cell B, it was confirmed that no leakage occurred even at a liquid supply pressure of 0.5 MPa at the stage where the screw was tightened to a tightening adjustment margin between the housing members of 0.25 mm (initially 0.4 mm). Further, even when a temperature of 240 ° C. was applied to the flow cell by a heater (not shown), the cell B constituting the cell did not break.

10: Flow cell (cell)
11: Sample liquid side flow path 12: Reference liquid side flow path 13: Swash plate 16: Sample liquid side flow path inlet 17: Sample liquid side flow path outlet 18: Reference liquid side flow path inlet 19: Reference liquid side Channel outlet 20: Sealing material (gasket)
30: Housing 31: Recess (Sample liquid side channel inlet side)
32: depression (sample liquid side channel outlet side)
35: Adjust the tightening 36: Pipe connection port from the analysis column 37, 39: Pipe connection port to the waste liquid line 38: Pipe connection port for supplying the eluent 40: Bolt 100: Flow cell 200: Eluent container 300: Liquid feed Pump 400: Pressure sensor 500: Resistance tube 600: Waste liquid container

Claims (3)

It has two hollow parts for allowing the reference liquid and the sample liquid, which are partitioned by a swash plate inclined with respect to the optical axis, to pass through the reference liquid or the sample liquid in a direction perpendicular to the optical axis. A rectangular parallelepiped cell provided with an inlet and an outlet
Piping connected to the inlet and outlet provided in the cell;
A sealing material made of gold for connecting the inlet and outlet provided in the cell and the pipe in a liquid-tight manner ; and
The flow cell, wherein the length of the inlet and outlet provided in the cell is 35% to 45% of the length between the side surfaces provided with the inlet and outlet . 2. The flow cell according to claim 1 , wherein an axis of an inlet and an outlet for passing a reference liquid and an axis of an inlet and an outlet for allowing a sample liquid to pass through are provided in the cell. Sample solution and the reference solution further comprising a housing provided with an internal flow path for inlet and outlet and for connecting the pipe for passing the flow cell of claim 1 or 2.

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