JP5441853B2 - Spatial odor indicator substance - Google Patents

Description

  The present invention relates to an indicator substance used for evaluating a smell with a stain in an aged room, a method for evaluating a stained odor in an aged room, and a method for evaluating a deodorizing effect on the odor.

  In recent years, with the increase in the elderly population, the number of elderly households has increased. Many elderly people tend to go out less frequently due to physical and social factors, and windows tend to be less opened and closed due to cold and crime prevention reasons. Therefore, a long time is often spent in the living room without opening the window, and there is a problem that odor peculiar to elderly people stays in the living room.

  In patent document 1, the whole odor which drifts in the room of a bedridden elderly person is analyzed, and from that, all sulfur compounds, all nitrogen compounds, acetic acid (carbon number 2), propionic acid (carbon number 3), iso, and normal butyric acid (carbon number) 4) Pivalic acid (5 carbon atoms), iso and normal valeric acid (5 carbon atoms) were detected, and these fatty acids were used for performance evaluation of the honeycomb activated carbon for adsorbing unpleasant odors. Although not clearly shown in the text, it is considered that dimethyl sulfide is the main component as the sulfur compound, and ammonia and indole are the main components as the nitrogen compound.

  Moreover, in patent document 2, octenal and nonenal are mentioned as a causative substance of the age-related odor emitted from a middle-aged and elderly person's body. In Non-Patent Document 1, isovaleric acid is regarded as an odor component of sweat odor and aging odor. Non-Patent Document 2 describes acetic acid, isovaleric acid, and n-caproic acid (hexanoic acid: carbon number 6) as odor components in the elderly living room.

  On the other hand, regarding the smell with a stain in the living space, Patent Document 3 discusses an evaluation method for tobacco odor.

JP-A-6-319932 Japanese Patent Laid-Open No. 11-286428 Japanese Patent Laid-Open No. 4-98157

Textile Evaluation Technology Council HP deodorant processed fiber product certification standard (http://www.sengikyo.or.jp/cmsdesigner/dlfile.php?entryname=sek&entryid=00004&fileid=00000016&/JED301%20%BE% C3% BD% AD% B2% C3% B9% A9% C1% A1% B0% DD% C0% BD% C9% CA% C7% A7% BE% DA% B4% F0% BD% E0% 20100401.doc) Megumi Megumi et al., 14th Annual Meeting of Odor Environmental Society, p.88, Study on Odor Generation in Living Rooms in Elderly Facilities

  Among the odors unique to such elderly people's rooms, the most recent problem is that even if the air in the room is ventilated, the odor that has adhered to and accumulated on the walls is re-released. This is a phenomenon in which the odor is reproduced, and even the causative substance has not been clarified. Knowing the causative components of stained odors detected in the rooms specific to elderly people is important for developing effective deodorants, indicators for developing air purifiers, and building materials that do not easily adhere to odors. Yes, elucidation is desired from each field. However, there is no analysis example focusing on the odor of the elderly in the living space from the quality of the odor, and the odor component that causes it is not known.

  Even in Patent Document 1 and Non-Patent Document 2 that analyzed elderly people's rooms, there is no suggestion as to which of the fatty acids described in these documents is a component that easily permeates.

In addition, among the odor components described in Patent Document 1 and Patent Document 2, components other than indole have a high vapor pressure, and are easily discharged by ventilation and cannot be detected as odor, or diffuse in a space with less odor. As a result, the sensory strength declines over time, and it is difficult to think of it as a component of a odor with a stain (reference; vapor pressure at 25 ° C is ammonia 2.2 × 10 ¹¹ Pa, dimethyl disulfide 3.3 × 10 ³ Pa, Acetic acid 2290Pa, isovaleric acid 152Pa, nonenal 42Pa, hexanoic acid 37Pa. Calculated using Estimation Program Interface (EPI) Suite Ver.4.0 of US ENVIROMENTAL PROTECTION AGENCY).

  Indole is solid at room temperature (vapor pressure at 25 ° C is 3 Pa) and is not easily discharged by ventilation. ) Is not suitable as an indicator material for unpleasant odors.

  An object of the present invention is to provide an indicator substance that enables objective and quantitative evaluation of a stained odor in an elderly person's room, a method for evaluating the odor using the same or a deodorizing effect on the odor, and an actual elderly The object is to provide a pseudo odor composition that accurately reproduces the odor of a living room.

  As a result of analyzing various odor components in an elderly patient room with significant odor staining, the present inventors have found that a fatty acid having 7 to 9 carbon atoms is particularly detected in comparison with a conference room on the same floor ( See Reference Example 1). The cause of these fatty acids is not clear, but it becomes a causative agent of the odor of a living space due to various factors such as low vapor pressure (in relation to indoor airflow), adsorption / desorption to the wall, and low threshold. It is estimated that On the other hand, no clear difference was observed in aldehydes such as Nonenal as described in Patent Documents 2 and 3, and fatty acids having 2 to 5 carbon atoms were detected in the conference room.

  From these results, the present inventors have conceived that the saturated fatty acid having 7 to 9 carbon atoms is used as an objective index for quantitatively evaluating the stained odor of the elderly's living room, and further studied, We succeeded in obtaining a pseudo odor composition that accurately reproduces the smell of aged people's living room using fatty acids, and a method for evaluating the deodorizing effect on the old person's living room.

  That is, the present invention provides an aged room-stained odor indicator substance containing at least one selected from saturated fatty acids having 7 to 9 carbon atoms.

  Furthermore, the present invention provides a method for evaluating odors of elderly people's room using the saturated fatty acid as an index.

  Furthermore, this invention provides the evaluation method of the deodorizing effect with respect to the aged person's room smell with the said saturated fatty acid as a parameter | index.

  Furthermore, this invention provides the pseudo-odor composition of the elderly person's room smell with a saturated fatty acid of 7-9 carbon atoms.

  The saturated fatty acid used in the present invention has an odor very close to an aged room-stained odor, and is useful as an indicator substance for the odor. Therefore, based on the amount of the saturated fatty acid, it is possible to objectively and quantitatively evaluate the degree of the odor of aged people or the deodorizing effect on the odor.

  Here, in the present invention, “elderly living room” means a room where an elderly person lives for one week or longer, and may be a home, a hospital, an elderly facility, The form is not limited.

  In the present invention, “smell with aged living room” is an odor that adheres to and accumulates on the walls, floors, bedding, curtains, etc. of the aged living room, and is gradually released, and the room air is completely replaced. It is re-released even if ventilated and does not disappear easily. Hereinafter, it may be simply referred to as “stained odor”.

  The “deodorizing effect” in the present invention includes: 1. Physical deodorization effect using odor adsorption or inclusion by deodorant base, etc. 2. Chemical deodorization effect using odor neutralization, oxidation, etc. with deodorant base, etc. Any sensory deodorizing effect using a fragrance masking agent or a modulator is included.

Stained odor indicator substance The odor indicator substance of the present invention contains a saturated fatty acid having 7 to 9 carbon atoms, and the saturated fatty acid may be either linear or branched. The straight-chain saturated fatty acid is heptanoic acid, octanoic acid or nonanoic acid, and examples of the branched saturated fatty acid include isoheptanoic acid, isooctanoic acid and isononanoic acid. Of these, linear saturated fatty acids, that is, heptanoic acid, octanoic acid, and nonanoic acid are preferred.

  When these saturated fatty acids having 7 to 9 carbon atoms are used as a stained odor indicator substance, they may be a single substance or a mixture of a plurality of saturated fatty acids. Moreover, although the purity is so preferable that it is high, it may contain impurities as long as it does not affect the odor.

[Derivatives of saturated fatty acids]
The saturated fatty acid having 7 to 9 carbon atoms may be used after chemical modification, that is, by introducing an atom or an atomic group into the carboxy group, as long as the detection function as an indicator compound is not lost. For example, in order to improve analytical sensitivity in instrumental analysis, the indicator substance is visually observed by acylating, esterifying, trimethylsilylating, amidating, carboxylating the carboxy group, or irradiating light in the visible region or ultraviolet region. A chromophore can be introduced into the carboxy group in order to make it possible.

  Derivatizing reagents include UV reagents such as O- (p-nitrobenzyl) -N, N'-diisopropylisourea (PNBDI) and p-bromophenacyl bromide (PBPB), 4-bromomethyl-7-methoxycoumarin Fluorating reagents such as (Br-MmC), silylating agents such as N-trimethylsilylimidazole (TMSI) and N, O-bis (trimethylsilyl) acetamide (BSA), acylating agents such as trifluoroacetic anhydride and trifluoroacetylimidazole Etc. can be used.

  When a chromophore in the visible region is used as a labeling compound for saturated fatty acids having 7 to 9 carbon atoms, a concentration-coloring standard sample of the labeling compound is prepared, and the odorant extract collected from the sample is colored with the same reagent. It is also possible to visually judge the degree of a smell with a stain as compared with the above.

As a method of determining the presence or abundance of saturated fatty acids using a color reaction,
i) A method of directly introducing a chromophore into a carboxy group of a saturated fatty acid having 7 to 9 carbon atoms
ii) A method of introducing a chromophore into a derivative after converting a saturated fatty acid having 7 to 9 carbon atoms into the derivative.
iii) A method of introducing a chromophore into the decomposed product after decomposing the saturated fatty acid having 7 to 9 carbon atoms.

  Coloring reagents used in the method of i) introducing a chromophore directly into the carboxy group of a saturated fatty acid include a reagent that introduces a saturated fatty acid into a chromogenic acid hydrazide in the presence of a condensing agent, and a saturated fatty acid. There are a reagent for introducing a color to a chromogenic ester and a color, a reagent for introducing a saturated fatty acid to a chromogenic amide and a color.

  Reagents for introducing saturated color of saturated fatty acid to chromogenic acid hydrazide include 2-nitrophenylhydrazine, 2,4-dinitrophenylhydrazine, 6,7-dimethoxy-1-methyl-2 (1H) -quinoxalinone-3 -Propionylcarboxylic acid hydrazide (DMEQ-H), p- (4,5-diphenyl-1H-imidazol-2-yl) -benzohydrazide, p- (1-methyl-1H-phenanthro- [9,10-imidazole- 2-yl) -benzohydrazide, p- (5,6-dimethoxy-2-benzothiazoyl) -benzohydrazide and the like.

  Reagents for introducing saturated color of saturated fatty acids into chromogenic esters include 9-anthryldiazomethane, 1-naphthyldiazomethane, 1- (2-naphthyl) diazoethane, 1-pyrenyldiazomethane, 4-diazomethyl-7-methoxy. Coumarin, 4-bromomethyl-7-methoxycoumarin, 3-bromomethyl-6,7-dimethoxy-1-methyl-2 (1H) -quinoxalinone, 9-bromomethylacridine, 4-bromomethyl-6,7-methylenedioxycoumarin N- (9-acridinyl) -bromoacetamide, 2- (2,3-naphthylimino) ethyl trifluoromethanesulfonate, 2- (phthalimino) ethyl trifluoromethanesulfonate, N-chloromethylphthalimide, N-chloromethyl-4-nitro Examples include phthalimide, N-chloromethyl isatin, O- (p-nitrobenzyl) -N, N′-diisopropylisourea and the like.

  Examples of the reagent that introduces saturated fatty acid into a chromogenic amide to cause coloration include monodansylcadaverine, 2- (p-aminomethylphenyl) -N, N′-dimethyl-2H-benzotriazol-5-amine, and the like. .

  In the method of converting a saturated fatty acid of ii) into a derivative and then introducing a chromophore into the derivative, examples of the saturated fatty acid derivative that can be used for the color reaction include inorganic salts and acid chlorides.

  Saturated fatty acid inorganic salts can be reacted with aromatic halogens to give chromogenic esters and acid chlorides to chromogenic amides.

  Examples of the method for converting the saturated fatty acid into an inorganic salt include a method in which the saturated fatty acid is mixed with an alkaline substance such as a sodium hydrogen carbonate solution, a sodium carbonate solution, a sodium hydroxide solution, or a potassium hydroxide solution and neutralized. Aromatic halogens that can react with inorganic salts of saturated fatty acids to give chromogenic esters include p-nitrobenzyl bromide, phenacyl bromide, p-chlorophenacyl bromide, p-bromophenacyl bromide, p-iodophena Examples include silbromide, p-nitrophenacyl bromide, p-phenylphenacyl bromide, p-phenylazophenacyl bromide, N, N′-dimethyl-p-aminobenzeneazophenacyl chloride.

  Examples of the method for converting saturated fatty acid to acid chloride include a method of reacting saturated fatty acid with oxalyl chloride. Examples of the method for converting the acid chloride to a color-forming amide include a method of reacting with 9-aminophenanthrene in the presence of triethylamine.

  After degrading the saturated fatty acid of iii), the chromophore can be introduced into the degradation product by acyl-CoA synthetase in the presence of adenosine triphosphate (ATP) and coenzyme CoA in the saturated fatty acid. To produce acyl-CoA, then treated with acyl-CoA oxidase to produce enoyl-CoA and hydrogen peroxide, which is further treated with catalase to form aldehydes. There is a method in which a color reagent, 4-amino-3-hydrazino-5-mercapto-1,2,3-triazole (AHMT), is reacted to colorize the resulting purple color.

  Thus, in the present invention, the reagent used for the color reaction of the saturated fatty acid is not particularly limited as long as it reacts with any one of the saturated fatty acid, the saturated fatty acid derivative, and the saturated fatty acid decomposition product to develop a color.

Method for Evaluating Stained Odor or Deodorizing Effect According to the present invention, it is possible to quantitatively evaluate the degree of a stained odor or the degree of the deodorizing effect on the odor using these indicator substances.

  The method of using the saturated fatty acid having 7 to 9 carbon atoms as the index substance for odor evaluation is not particularly limited, and may be used in accordance with various known evaluation methods.

  For example, when using GC-MS to measure the abundance of saturated fatty acids with 7 to 9 carbon atoms in a living room with an odor, a calibration curve is created using the saturated fatty acids as standard substances (standards) in advance. To do. Using this calibration curve, the peak of saturated fatty acid contained in a sample collected from the elderly's living room may be identified and the amount thereof measured.

  When collecting a sample from the elderly's room, first ventilate the room, and then collect it after the room is closed and the odor that has penetrated the room has been sufficiently released. The ventilation time is not particularly limited as long as the air in the room can be completely replaced, but is preferably about 2 to 5 hours. Also, the time for closing the room after ventilation is not particularly limited as long as it is sufficient for the odor to be re-released, but about 2 to 5 hours is preferable. While the room is closed and odors are released again, other odor sources are released again without bringing them into the room.

  Moreover, when performing sensory evaluation, a C7-9 saturated fatty acid is diluted in several steps, and the odor standard sample of each density | concentration is prepared. And the odor of the evaluation sample collected from the elderly person's room with a smell with a stain is collated with a standard sample, and the amount of saturated fatty acid in the elderly person's room may be judged by sensory evaluation.

  As described above, the indicator substance of the present invention can be used for any of chemical analysis, instrumental analysis, and sensory evaluation, and can be quantitatively determined with high objectivity. It is possible to exclude the subjectivity from the determination result by expressing the value with the amount of saturated fatty acid.

  Further, in the present invention, the deodorizing effect of a deodorant or air purifier that targets a stained odor is objectively and quantitatively determined using an indicator substance containing a saturated fatty acid having 7 to 9 carbon atoms. Can do.

  In the method for judging the deodorizing effect of the deodorant or the air cleaner, the indicator substance may be used as a simple substance, other components such as a solvent for dissolution or dilution, a stabilizer, an antibacterial agent, You may mix | blend additives, such as an antibacterial agent, surfactant, antioxidant, a fragrance | flavor, and a plant extract, and you may prepare and use for the composition according to practical use, such as a use in a preservation | save or a judgment test.

For example, an indicator substance containing a saturated fatty acid having 7 to 9 carbon atoms as an active ingredient in a predetermined concentration is attached to the walls, floors, curtains, etc. of a living room, a predetermined amount of deodorant sample is added, and the indicator substance changes Can be objectively and quantitatively determined by quantifying the amount of odor by an appropriate method. The deodorizing effect of the air cleaner can be objectively and quantitatively determined by the same method.
Furthermore, by using the indicator substance of the present invention, it is possible to evaluate the stain-proofing odor control effect applied to building materials such as indoor fabric products, furniture, and wallpaper.

  When using the stained odor indicator substance of the present invention for the evaluation of a deodorant or an air cleaner, about 0.1 to 1 g as a saturated fatty acid having 7 to 9 carbon atoms per test sample is added by dropping or spraying, a filter paper, Infiltrate sheets such as non-woven fabric and wallpaper. Thereafter, it is preferable to leave the sheet infiltrated with the indicator substance in a room of about 2 to 6 tatami mats for about 2 hours to 1 day to volatilize and fill the indicator substance in the room. When leaving a sheet infiltrated with an indicator substance in a room, for example, a sheet in which an indicator substance has penetrated may be placed in a petri dish and left in the room, or a sheet infiltrated with an indicator substance may be attached to a wall surface. Also good.

  As a method for quantifying the state of change of the indicator substance, if the deodorant sample is a type that degrades saturated fatty acids or induces another compound to reduce odor, prepare a calibration curve for the indicator substance in advance. In addition, the analytical analysis may be performed using this calibration curve, or the changed or unchanged substance of the indicator substance may be quantified by chemical analysis such as titration or extraction. If the deodorant sample is of the type that masks the odor, the indicator substance is diluted in several stages to prepare odor standard samples of each concentration, and the odor of the indicator substance with the deodorant sample added is the standard sample. The masking effect may be determined by sensory evaluation.

  In addition, when evaluating the effect of stain odor control on building materials such as indoor fabric products, furniture, and wallpaper, about 0.1 to 1 g of saturated fatty acid having 7 to 9 carbon atoms per test sample is dropped or sprayed. Infiltrate sheets such as filter paper, non-woven fabric, and wallpaper. After that, leave the sheet with the indicator substance in a room where fabric materials, furniture, wallpaper, etc., which have been given a stain-proofing odor control effect, are left in a room of 2 to 6 tatami mats for about 2 hours to 1 day. It is preferable to volatilize and fill the room. When leaving a sheet infiltrated with an indicator substance in a room, for example, a sheet in which an indicator substance has penetrated may be placed in a petri dish and left in the room, or a sheet infiltrated with an indicator substance may be attached to a wall surface. Also good.

  When collecting samples for the evaluation of stain odor control effect, after sufficiently filling the indicator material in the room as described above, ventilate until there is no odor in the room, then fabric products, furniture, wallpaper You may extract an odor component directly from building materials, such as. Further, after ventilation, the room may be closed again, the indicator material once soaked may be re-released, and the re-released odor may be collected. As a method for quantifying the state of change of the indicator substance, if the odor control effect with stains is to reduce odor by degrading saturated fatty acids or inducing to another compound, a calibration curve for the indicator substance is prepared in advance. The analytical analysis may be performed using the calibration curve, or the changed or unchanged substance of the indicator substance may be quantified by chemical analysis such as titration or extraction.

● Pseudo-stained odor composition Furthermore, the present invention provides a pseudo-stained odor composition that contains a saturated fatty acid having 7 to 9 carbon atoms and can be used for evaluating the deodorizing effect of the scented odor. By using this pseudo-stained odor composition, it is possible to accurately and reproducibly evaluate the deodorizing base screening and the deodorizing effect of the deodorant composition with respect to the stained odor.

  When this saturated fatty acid is used for odor determination in sensory evaluation, etc., a known odor odor component is mixed at an appropriate ratio to reproduce a odor more realistic than the actual situation, and a pseudo odor odor composition Can also be used.

  That is, the pseudo-stained odor composition of the present invention contains a saturated fatty acid having 7 to 9 carbon atoms as a component (A) from the viewpoint of more accurately reproducing a stained odor that is a composite odor containing various odors, Component (B) is not important as a odor indicator substance with a stain, or is a known malodorous substance, for example, a fatty acid having 2 to 6 carbon atoms (acetic acid, valeric acid, isovaleric acid, hexanoic acid, etc.), carbon number 8 ~ 10 aldehydes (octanal, nonanal, nonenal (aged odor), decanal, etc.), ammonia, etc. can be added. Among fatty acids having 2 to 6 carbon atoms, valeric acid, isovaleric acid, and hexanoic acid are preferable, and among aldehydes having 8 to 10 carbon atoms, octanal and nonenal are more preferable.

  In the present invention, the ratio (mass ratio) of component (B) to component (A) is preferably in the range of (B) / (A) from 0 to 1, more preferably (B) / (A) is from 0.01 to It is in the range of 0.5.

  Moreover, the pseudo-stained odor composition of the present invention is a solvent such as water, ethanol, triethyl citrate, acetone, propylene glycol, dipropylene glycol, liquid paraffin, LPG (liquefied petroleum gas) as a diluent from the viewpoint of handleability. In addition, a surfactant such as cationic, anionic, nonionic or amphoteric can be used in combination for dilution.

  The pseudo-stained odor composition of the present invention can be used in a liquid state or impregnated in a solid carrier. The solid carrier is not particularly limited as long as it can support the pseudo-stained odor composition, but for example, silica gel, silica, activated carbon, hydroxyapatite, alumina, zeolite, diatomaceous earth, clay mineral, cyclodextrin, talc, calcium carbonate , Gelling agent, cellulose and its derivatives, foamed cellulose, paper, cotton, wool, polyester fiber, nylon fiber, non-woven fabric, resin (polypropylene, polyethylene, polyester, nylon, polyurethane, polyvinyl, polyvinylidene, ethylene-polyvinyl alcohol copolymer) Coalesced, polystyrene, polyacrylate, allylstyrene copolymer) and the like. The form of the solid carrier is not particularly limited, and for example, it can be used as powder, granules, sheets, lumps and the like.

  In order to evaluate the deodorizing effect on the stained odor using the pseudo-stained odor composition of the present invention, for example, in a space filled with the odor, both a space using a deodorant and a space not used You can smell each odor and compare. Similarly, the odor intensity before and after spraying the deodorant may be recorded to determine the effect. An air purifier can be used for evaluation instead of the deodorant spray. The odor is preferably evaluated by 1 to 20 professional panelists, and is preferably evaluated in 3 to 10 stages.

Reference example 1
For the elderly room (4 people room) with noticeable odor and the meeting room without odor (both on the same floor. The wallpaper and building materials are the same), open the windows and ventilate for 3 hours. The state was not detected. Then, each room was closed for 3 hours, and then a odor sample was collected at a rate of 4.2 L / min x 3 hours using Tenax TA (GL Sciences, each filled with 2300 mg in 3 tubes). did. Various odor components were analyzed by a heating extraction device (Gerstel) and GC-MS (column: DB-1 (manufactured by J & W)) length 60m / inner diameter 0.25mm / film thickness 0.25μm, temperature rising condition: 40 ° C (3 min hold)-6 ℃ / min-60 ℃-2 ℃ / min-300 ℃).
Table 1 shows the difference in analysis values between the hospital room and the conference room.

  As a result, as can be seen from Table 1, it was found that a large number of linear fatty acids having 6 to 9 carbon atoms were detected in elderly patients' rooms with a noticeable odor compared to conference rooms. In particular, linear fatty acids having 7 to 9 carbon atoms were prominently detected in elderly patients' rooms. In contrast, fatty acids having 2 to 5 carbon atoms were detected more in the conference room, and no obvious difference was observed in the aldehyde.

Test example 1
The gas chromatographic analysis value correction was performed on the measurement value of “elderly patient room (stained smell strong)” in Reference Example 1.
Referring to the correction method described in Japanese Patent Application Laid-Open No. 2002-328078, the composition value obtained from the measurement values of the elderly patient room is divided by the vapor pressure of each component to obtain a correction composition with 100 as a whole, based on that A pseudo-stained odor composition was prepared.
With a 10mg of this pseudo-spotting odor composition to 3cm × 3cm of filter paper, to evaporate one day in the evaluation box of 2m ^3, it was evaluated the quality of the smell in the next day box, and very spotting smell of the field of the elderly facilities Confirmed that it is similar to.

Test example 2 Confirmation of similarity between the type of odor component and the odor with stains The "corrected composition" of Test example 1 (Table 2) was diluted 2 times by mass with dipropylene glycol (hereinafter referred to as DPG), which is an odorless solvent. The following corrected odor samples were prepared on the basis of (constitutive recipe) (all balanced with DPG).
・ Prescription A: odor component only in aldehyde in reconstitution prescription B: odor component only in saturated fatty acid having 3 to 6 carbon atoms in reconstitution prescription C: odor component in reconstitution prescription Aldehyde + Saturated fatty acid having 3 to 6 carbon atoms / Prescription D: The ratio of saturated fatty acid having 7 to 9 carbon atoms in the reconstitution formulation is halved.Prescription E: Carbon number 7 in the reconstitution formulation put to 9 of these odor samples each 10mg those twice the proportion of saturated fatty acids of the filter paper 3 cm × 3 cm, evaporated daily by the evaluation box 2m ^3, the odor of the next day box, the actual elderly A comparison was made from the viewpoint of the similarity with the odor of a facility. The evaluation panel consisted of 3 specialist panels working at the elderly facilities, and the final evaluation was decided by a joint discussion between the three.
As a result, as shown in Table 3, it can be seen that the odor of saturated fatty acids having 7 to 9 carbon atoms is particularly important as an index of a smell with a stain in an elderly room.

<Similarity to the odor of a living room for elderly people>
◎: Very similar ○: Very similar △: Slightly similar ×: Not similar at all

Test Example 3 Smell Rerelease Test (1)
10 mg amount of each of the odor samples F to K shown in Table 4 was dropped on a cotton ball having a diameter of 1 cm, and fixed to the bottom of a 100 mL bottle with 1 × 1 cm double-sided tape. When the odor was filled in the bottle, the odor strength at the bottle mouth was evaluated. The evaluation was made based on the criteria shown below, in consultation with three expert panels. As a result, the odor intensity at the initial bottle opening of the odor sample was approximately 3 to 4 according to the following criteria.
Subsequently, a 6 × 10 cm piece of polyester wallpaper was placed along the inner peripheral surface and capped. The bottle was left at 30 ° C. for 3 days, and then the wallpaper piece inside was taken out and left for 1 day in a room at 25 ° C. with the ventilation fan turned (this is regarded as a ventilation state).
The wallpaper piece was put again into a new 100 mL bottle, and the lid was capped to evaluate the odor re-released from the wallpaper after 2 days at 30 ° C. The evaluation was carried out based on the following criteria for the odor intensity at the bottle mouth and the similarity with the odor with a stain by the consultation of three specialist panels. The evaluation results are shown in Table 4.

<Evaluation criteria for odor intensity (6-level odor intensity display method; criteria in the Odor Control Act, judgment is made in 0.5 increments)>
5: Strong odor 4: Strong odor 3: Easily perceived scent 2: Weak scent that understands what scent is 1: Scent that can finally be perceived 0: Odorless

<Similarity to the odor of a living room for elderly people>
◎: Very similar ○: Very similar △: Slightly similar ×: Not similar at all

  All of these odor samples recalled a scented odor. Among them, the odor samples G, I, J and K reminded more of the odor.

Test Example 4 Odor re-release test (2)
The odor sample L is 1 mg of ammonia (effective amount), the odor sample M is dimethyl disulfide 3 mg, the odor sample N is acetic acid 3 mg, the odor sample O is nonenal 3 mg, the odor sample P is isovaleric acid 1 mg, and the odor sample Q is indole 10 mg. The test was conducted in the same manner as in Test Example 3.
The evaluation results are shown in Table 5.

  Ammonia, dimethyl sulfide, and acetic acid did not regenerate after ventilation. Although isovaleric acid had odor intensity in the regeneration test, the change in odor intensity was large. Nonenal and indole were observed to reoccur, but their resemblance to the actual odor was low.

Example 1 Evaluation of Deodorizing Effect Using Stained Odor Indicator Material in Elderly Living Rooms (1)
1-1. Preparation of test sample A solution in which octanoic acid was diluted 1000 times by mass with ethanol was prepared. 0.3 g of the above solution was dropped onto a 10 cm diameter polyester non-woven fabric placed in a glass petri dish with a diameter of 20 cm, and then allowed to stand for 3 hours to be used for evaluation of a odor with a stain.

1-2. Preparation of deodorant A water-ethanol (85% by mass / 15% by mass) 5% by mass solution of green tea extract (FS-500M, manufactured by Shiraimatsu Shinyaku Co., Ltd.) was prepared.

1-3. Evaluation criteria for odor strength The 6-step odor intensity display method used in Test Example 3 (standard in the Odor Control Act) was used.

1-4. Deodorization test
Prepare two petri dishes with octanoic acid solution prepared in 1-1, spray the deodorant prepared in 1-2 on one side only 24 times, let stand for 10 minutes, then stain the two petri dishes The intensity of odor was evaluated.
As a result, the strength of the odor of the petri dish that was not sprayed with the deodorant was 3, but the strength of the odor of the petri dish that was sprayed with the deodorant was lowered to 2, and there was a stain for evaluating the deodorizing effect. It was judged to be suitable as an odor indicator substance and a deodorizing effect evaluation method.

Example 2 Evaluation of Deodorizing Effect Using Stained Odor Indicator Material in Elderly Living Rooms (2)
2-1. Preparation of a sample for stain odor test 10 mg of a stain odor indicator substance of heptanoic acid / octanoic acid / isooctanoic acid / nonanoic acid = 10/60/10/20 (mass ratio), 7 mm wide, 15 cm long, 1 mm thick filter paper And impregnated with a sample for a odor test with a stain.

2-2. Preparation of Deodorant Sample A deodorant sample was prepared by impregnating 50 mg of orange oil into a filter paper having a width of 7 mm, a length of 15 cm, and a thickness of 1 mm.

2-3. Evaluation criteria for odor strength The 6-step odor intensity display method used in Test Example 3 (standard in the Odor Control Act) was used.

2-4. Deodorization Test Two sealed spaces of width 1 m × depth 1 m × height 2 m were prepared, and the stained odor test sample prepared in 2-1 was sandwiched between clips at the bottom of both spaces. Next, the deodorant sample prepared in 2-2 was further fixed to a clip and placed in one space. After 30 minutes, the odor intensity of each space was evaluated.
As a result, the odor intensity in the space where the deodorant sample was not installed was 3, but the odor intensity in the space where the deodorant sample was installed was lowered to 2, and the masking effect by the fragrance was evaluated. It was judged that it was suitable as a masked evaluation method and a odor indicator material with a stain for the space.

Example 3 Evaluation of Deodorizing Effect Using Stained Odor Index Substance in Elderly Living Room (3)
3-1. Preparation of Stained Odor Test Sample Impregnated wall paper of 6cm x 10cm width with 10mg of stained odor indicator substance of heptanoic acid / octanoic acid / nonanoic acid / 2-nonenal = 10/60/20/10 (mass ratio) A sample for odor test with stain was used.

3-2. Used air purifier A commercial air purifier (height 60 cm x width 40 cm x depth 25 cm) with normal coconut shell charcoal built in as a deodorizing filter was operated in medium mode (inlet wind speed 2.0 m / sec).

3-3. Evaluation criteria for odor strength The 6-step odor intensity display method used in Test Example 3 (standard in the Odor Control Act) was used.

3-4. Deodorization test A sample for odor test with 3-1 was fixed to one wall surface in a space of width 1 m × depth 1 m × height 2 m with a tape and left for 6 hours. The intensity of odor at that time was 2.5.
After that, 3-2 air purifier with the room closed
“10-minute operation → odor intensity evaluation → 3 hour stop → odor intensity evaluation → 10 minute operation → odor intensity evaluation → 3 hour stop → odor intensity evaluation”
The odor intensity was evaluated (deodorant effect was evaluated).
As a result, after the first 10 minutes of operation, the odor intensity decreased from 2.5 to 1, but after 3 hours of operation stop, the odor intensity became 2 again and decreased to 1 after the operation again. Thus, it can be seen that the use of the odor indicator material with a stain of the present invention enables evaluation of the effect of the air cleaner (short time, continuous operation, estimation of effective space) and comparison between products.

Claims (7)

  An elderly odor indicator odor indicator material containing at least one selected from saturated fatty acids having 7 to 9 carbon atoms.   The saturated fatty acid is a linear fatty acid.   The evaluation method of the odor with which the elderly person's room stains using the saturated fatty acid of Claim 1 or 2 as a parameter | index.   4. The method for evaluating an odor of an elderly person's room according to claim 3, further comprising the step of: closing the room after the ventilation of the elderly person's room, and then sampling the air in the elderly person's room.   The evaluation method of the deodorizing effect with respect to the odor with which the elderly person's room stains which uses the saturated fatty acid of Claim 1 or 2 as a parameter | index.   A pseudo-odor composition of an aged room-stained odor containing a saturated fatty acid having 7 to 9 carbon atoms.   Furthermore, the pseudo odor composition of Claim 6 containing 1 or more types chosen from a C2-C6 fatty acid, a C8-C10 aldehyde, and ammonia.