JP5401978B2 - Method and apparatus for measuring lysate concentration - Google Patents

Description

  The present invention relates to a lysate concentration measuring method and a measuring apparatus for measuring a lysate concentration in a sample based on light transmittance or absorbance. The present invention also relates to a color tone detection method and a detection apparatus for detecting the color tone of the liquid to be detected based on the light absorbing action of the liquid to be detected.

  In many cases, the concentration of the dissolved substance dissolved in the liquid is determined by absorptiometry. In this absorptiometric method, for example, a reagent is added to a sample having a lysate, and the sample is colored to form a liquid to be measured, and then the liquid to be measured is accommodated in a transparent measurement cell. Next, the liquid to be measured is allowed to transmit a specific wavelength of light having a high degree of absorption from the illuminant through the measurement cell, and a part of the light is absorbed by the liquid to be measured. The light is received by the photoreceptor, and the transmitted light intensity at this time is measured. Subsequently, by obtaining the absorbance (or transmittance) from the transmitted light intensity measured at this time and the transmitted light intensity of light having a specific wavelength with respect to the transparent liquid measured separately, for example, a sample having a known concentration in advance with respect to this lysate. Using the calibration graph showing the relationship between the absorbance (or transmittance) and the lysate concentration created by the above, the lysate concentration in the sample is determined.

  Here, when investigating the concentration of a plurality of lysates of different types, the concentration of the lysate is measured using a plurality of light emitters that emit light of different wavelengths (see Patent Document 1), or a light source. The concentration of the lysate is measured using a light emitter that can adjust the light from the light to a plurality of specific wavelengths. In addition, even when the liquid to be measured changes color depending on the concentration of the dissolved matter, the concentration of the dissolved matter is measured using a plurality of light emitters that emit light having different wavelengths.

JP 2006-257553 A

  However, when a plurality of light emitters having different wavelengths are used, a plurality of light receivers are required, resulting in an increase in cost and size of the apparatus. Further, when a plurality of light emitters are used at the same time, there is a problem that light easily interferes with each other, and an error is likely to occur in a measured value. In addition, when an LED with a specific wavelength is used as the light emitter, the wavelength of the light varies depending on the production lot, and is likely to fluctuate depending on the output level. It was.

  In addition, when a light emitter that can adjust light from a light source to a plurality of specific wavelengths is used, there is a problem in that the cost and size of the apparatus are increased.

In view of the above points, the present invention provides a measurement method and a measurement apparatus for a lysate concentration that can achieve cost reduction and downsizing of the apparatus when measuring the lysate concentration in a sample based on the absorbance of light. For the purpose.

According to the first aspect of the present invention, a liquid to be measured which is colored by the addition of a reagent to the sample or a sample colored by a lysate, and a liquid to be measured which does not absorb light, Calculated by transmitting light from the light emitter to each other and receiving the transmitted light with a light receiver placed opposite the light emitter so as to sandwich the liquid to be measured. A lysate concentration measurement method for measuring a concentration of a specific lysate in the sample based on the absorbance of the transmitted light with respect to the colored liquid to be measured , wherein the illuminant includes a visible light region. The light receiver emits light, and the light from the light emitter that has passed through the liquid to be measured is obtained by substantially dividing the light in the visible light region into three parts. component of the light, or blue regions ingredient either light If either light or respectively receive light of a plurality of said regions ingredients a combination of these, and, where the measured fluid, wherein with a change in concentration of a particular lysate changing the hue, a plurality The concentration of the specific lysate is calculated using the absorbance value calculated for the light of the region component, and the hue of the liquid to be measured changes with the change in the concentration of the specific lysate. If not, the concentration of the specific lysate is calculated using the absorbance value calculated for the light of one region component .

In the present invention, the light receiver is obtained by substantially dividing the wavelength band of light in the visible light region out of the light from the light emitter transmitted through the liquid to be measured, and is obtained by dividing the light of the red region component and the light of the green region component. And blue region component light are received, and the intensity of the light is measured. For example, when coloring in yellow by test liquid specific lysate by addition of the reagent, the photoreceptor, blue is a complementary color of yellow, that is, the intensity of the light blue region components, coloring in yellow the the test solution and by measuring the absorption with no clear target solution of the light, using these values, by calculating the absorbance of the test liquid coloring in yellow for the light blue region components, specific sample The concentration of the lysate can be easily determined.

Further, in the present invention, the light receiver is obtained by substantially dividing the wavelength band of light in the visible light region out of the light from the light emitter that has passed through the liquid to be measured, and the light of the red region component and the green region component. The light of a plurality of color region components, which is a combination of the light of the blue region component and the light of the blue region component, is received, and the intensity of each light is measured. For example , when the color of the solution to be measured changes from a red complementary color to a blue complementary color depending on the concentration of a specific lysate , the light of the red region component and the blue region component are changed by the photoreceptor. The intensity of light is measured for a colored liquid to be measured and a transparent liquid to be measured that does not absorb light. Using these values, the absorbance of the colored liquid to be measured is calculated as the red region component. By calculating the light and the light of the blue region component, the concentration of a specific lysate in the sample can be easily obtained. Of course, when the color of the liquid to be measured varies depending on the concentration of the specific lysate , the photoreceiver receives the red region component light, the green region component light, and the blue region component light. Then, the light absorbance of each of the three region components is calculated to determine the concentration of a specific lysate in the sample .

According to a second aspect of the present invention, there is provided a liquid to be measured that is colored by addition of a reagent to the sample or a sample that is colored by a lysate, a liquid to be measured that does not absorb light, Calculated by transmitting light from the light emitter to each other and receiving the transmitted light with a light receiver placed opposite the light emitter so as to sandwich the liquid to be measured. A lysate concentration measuring device that measures the concentration of a specific lysate in the sample based on the absorbance of the transmitted light with respect to the liquid to be measured , wherein the illuminant includes a visible light region. The light receiver emits light, and the light from the light emitter that has passed through the liquid to be measured is obtained by substantially dividing the light in the visible light region into three parts. component of the light, or blue regions ingredient either light Whether the filter for the bulk, or have a filter for transmitting each light in the plurality of regions ingredients a combination thereof, and the measured liquid, with a change in the concentration of the specific lysate In the case of changing the hue, the concentration of the specific lysate is calculated using the absorbance values calculated for the light of the plurality of region components, and the measured liquid is the specific lysate. In the case where the hue does not change with the change in the concentration of the light source, it has an arithmetic processing unit that calculates the concentration of the specific lysate using the absorbance value calculated for the light of one region component. It is characterized by.

  According to the first and second aspects of the present invention, a simple pair of a light emitter and a light receiver is sufficient for measuring the concentration of any lysate. Reduces measurement costs by eliminating the need to use multiple sets of illuminants and photoreceptors when measuring lysates with different wavelengths for each type of lysate, or when measuring lysate concentrations that change color depending on the concentration In addition, the size of the measuring device can be reduced. For this reason, the mutual interference of the light which arises when using several light-emitting body simultaneously is not produced. In addition, according to the present invention, the light emitter need only emit light including a visible light range, and it is not necessary to emit only light of a specific wavelength unlike the conventional one. It is difficult to be affected by wavelength variations among lots and changes in output over time, and can always perform highly accurate measurements.

Embodiments of the present invention will be described below with reference to the drawings.
Embodiment 1. FIG.
FIG. 1 shows a main part of a concentration measuring apparatus according to an embodiment of the present invention, and FIG. 2 is an explanatory view of the operation of the concentration measuring apparatus.

The concentration measuring apparatus 1 can be used to easily determine the concentration of a dissolved material such as dissolved oxygen, phosphoric acid, alkalinity component, hardness component, and silica dissolved in industrial water or domestic water, using light transmittance and absorbance. Measure. As shown in FIG . 1 and FIG. 2 , the concentration measuring device 1 is provided on the measurement cell 2 through which the liquid S1 to be measured and the adjustment liquid S0 are passed, and on one side of the measurement cell 2. A light emitter 3 that emits measurement light in the measurement cell 2; a light receiver 4 that is provided on the other side surface of the measurement cell 2 and receives light from the light emitter 3 that has passed through the measurement cell 2; The input / output unit 5 for the body 3 and the light receiving body 4, the liquid supply line 6 for supplying the measurement liquid S1 and the adjustment liquid S0 to the measurement cell 2, and the measurement liquid S1 and the adjustment liquid S0 from the measurement cell 2 are discharged. A liquid discharge line 7 and an arithmetic processing unit 8 to which an output signal (transmitted light intensity signal) from the input / output unit 5 is input are provided. The liquid supply line 6 is provided with a tube pump 60 and a strainer 61.

As shown in FIGS. 1 and 2, the measurement cell 2 has a constant cross section through which the measured liquid S <b> 1 and the adjustment liquid S <b> 0 are passed between the left side surface portion 21, the right side surface portion 22, the front surface portion, and the rear surface portion. While the flow path 20 is formed, the liquid supply line 6 is connected to the lower surface portion, and the liquid discharge line 7 is connected to the upper surface portion. Further, in the measurement cell 2, circular transparent portions 21 a and 22 a are formed at positions facing each other in the central portion of the left side surface portion 21 and the right side surface portion 22. Then, for example, the light emitter 3 is disposed on the transparent portion 22a side of the measurement cell 2, the optical axes are aligned so as to face the light emitter 3, and the light receiver 4 is disposed on the transparent portion 21a side of the measurement cell 2. Is arranged.

  The illuminant 3 emits light into the measurement cell G and transmits this light into the measured liquid S1 and the adjustment liquid S0 in the measurement cell 2. For the light emitter 3, a light source such as a light emitting diode (LED) that emits light including white light (white light) is used.

The light receiver 4 receives the transmitted light emitted from the light emitter 3 and transmitted through the measurement cell 2 through the measurement liquid S1 and the adjustment liquid S0, and measures the intensity of the transmitted light. is there. The photoreceptor 4 includes three photodiodes, light of a red region component (hereinafter referred to as red band light), and light of a green region component (hereinafter referred to as green) obtained by substantially dividing the wavelength band of light in the visible light region into three. that band light), or three color filters F which transmits a blue region component light (hereinafter referred to as the blue band light) only, respectively, i.e., red (R) filter, a green (G) filter and a blue (B) filter Have. That is, an RGB color sensor having a photodiode D1 having an R filter, a photodiode D2 having a G filter, and a photodiode D3 having a B filter is used for the photoreceptor 4. 3), the light intensity of each of the red band light, the green band light, and the blue band light transmitted through each filter among the light transmitted through the liquid S1 to be measured is measured simultaneously. The The R filter transmits most red light in the red band light, the G filter transmits most green light in the green band light, and the B filter transmits blue light most in the blue band light.

  The input / output unit 5 includes a control circuit for the light emitter 3 and the light receiver 4. FIG. 3 is a circuit diagram in the input / output unit 5 including the light emitter 3 and the light receiver 4. In the figure, reference sign D1 is a photodiode having an R filter, reference sign D2 is a photodiode having a G filter, reference sign D3 is a photodiode having a B filter, and these are integrated. Thus, the photoreceptor 4 is formed. Further, in the figure, symbol L is a light emitting diode (LED) that becomes the light emitter 3, symbols C1, C2, and C3 are main circuits for the photodiodes D1, D2, and D3, and symbols O1, O2, and so on. O3 is an operational amplifier (operational amplifier) for each of the photodiodes D1, D2, and D3. A signal of transmitted light intensity of each band light output from the light receiving body 4 is transmitted to the arithmetic processing unit 8 through the operational amplifiers O1, O2, and O3.

  The arithmetic processing unit 8 calculates the time average intensity for each band light based on the intensity signals of the light of the red band light, the green band light, and the blue band light output from the light receiver 4, or the specific color is Using the transmitted light intensity of absorbed light and the transmitted light intensity of non-absorbed light, the absorbance and transmittance of red band light, green band light and blue band light can be calculated, or red band light and green light can be calculated. It has a calculation unit that calculates the concentration of lysate from each absorbance and transmittance of band light and blue band light, and for each type of lysate, each absorbance of red band light, green band light, and blue band light It has a memory | storage part which memorize | stores the table | surface etc. which show the relationship between the transmittance | permeability and a melt | dissolution density | concentration, and a display part which displays a melt | dissolution density | concentration etc.

Next, a procedure for measuring the concentration of dissolved matter (for example, phosphate ions) in the sample using the concentration measuring apparatus 1 will be described.
First, after adding a certain amount (for example, 4 ml) of a reagent (a mixture of ammonium molybdate and astellic acid) to a certain amount (40 mL) of a sample in which trisodium phosphate (Na ₃ PO ₄ ) is dissolved, The solution to be measured S1 is made by allowing the sample to stand for about 15 minutes at 20 to 40 ° C. and causing sufficient color development. In this case, the color of the liquid S1 to be measured varies depending on the concentration of the dissolved substance (phosphate ion).

  Subsequently, a transparent adjustment liquid S0 (for example, pure water or a transparent sample before color development) that does not absorb light is measured from the liquid discharge line 6 using a tube pump 50 (for example, SMP21 manufactured by EYELA). After passing water through the cell 2 at a flow rate of 10 mL / min for about 3 minutes, the water flow was stopped and light from the light emitter 3 was passed through the transparent portions 21a and 22a of the measuring cell 2 for 1 minute. Irradiate (fire) during S1. As a result, the light including the visible light region from the light emitter 3 passes through the liquid S1 to be measured and is received by the light receiver 4. In this case, the light receiver 4 receives the transmitted light of the liquid S1 to be measured from the light emitter 3 through the three color filters F of RGB, so the light receiver 4 has a wavelength band of light in the visible light range. The intensity of each of the red band light, the green band light, and the blue band light divided into approximately three is measured simultaneously. Then, the arithmetic processing unit 8 averages the output values from the photoreceptor 4 over one minute, and the red band light and the green band light when there is no light absorption by the liquid S1 to be measured (transmittance 100%). The average light intensity of each blue band light is calculated.

  Next, the reagent is added and allowed to stand for a certain period of time, and the liquid S1 that has sufficiently developed color is passed through the measuring cell 2 at a flow rate of 10 mL / min for 3 minutes in the same manner as in the case of the adjustment liquid S0. The water is stopped, and light from the light emitter 3 is transmitted through the liquid S1 to be measured and received by the light receiver 4 for one minute thereafter. The light receiver 4 measures the intensity of each of the red band light, the green band light, and the blue band light that has been partially absorbed by the liquid S1 to be measured during light reception. The arithmetic processing unit 8 averages the output values from the photoreceptor 4 over 1 minute, and each of the red band light, the green band light, and the blue band light in which a part of the light is absorbed by the liquid S1 to be measured. After calculating the average light intensity of the red band light, the average light intensity of each of the red band light, the green band light, and the blue band light measured using the adjustment liquid S0 having a transmittance of 100% is used. The respective absorbances (or transmittances) of the green band light and the blue band light are calculated. The arithmetic processing unit 8 stores the absorbance (or transmittance) and the concentration of the lysate for the red band light, the green band light, and the blue band light, which are stored for the specific lysate (phosphate ions). From the relationship, the concentration of the lysate in the sample is calculated and displayed.

  Here, the liquid S1 to be measured shows, for example, a color depth proportional to the concentration of the dissolved matter, and absorbs the complementary color light of the colored light that has been developed at a rate proportional to this concentration. Therefore, the relationship between the absorbance (or transmittance) of the red band light, the green band light, and the blue band light among the light transmitted through the measured liquid S1 and the concentration of the dissolved substance in the measured liquid S1 is expressed as follows. If obtained in advance, the concentration of the lysate in the sample can be easily calculated from the respective absorbances (or transmittances) of the red band light, the green band light, and the blue band light transmitted through the liquid S1 to be measured.

  When the liquid S1 to be measured is colored yellow, for example, by adding a reagent, the liquid S1 to be measured hardly absorbs red band light and green band light, and is blue band light that is complementary color light. Therefore, it is necessary to consider only the blue band light measured by the photodiode provided with the B filter. Further, when the liquid S1 to be measured is colored blue, for example, the liquid S1 to be measured hardly absorbs blue band light and absorbs red band light and green band light. The intensity may be determined in consideration of the intensity of red band light and green band light measured by a photodiode having an R filter and a G filter.

  Further, the color of the liquid S1 to be measured may change gradually from a specific color to another color, for example, according to the concentration of the dissolved matter. Even in this case, the relationship between the respective absorbances (or transmittances) of the red band light, the green band light, and the blue band light transmitted through the measurement liquid S1 and the concentration of the dissolved substance in the measurement liquid S1 is previously determined. Therefore, the concentration of the dissolved substance in the measured liquid S1 can be easily calculated from the respective absorbances (or transmittances) of the red band light, the green band light, and the blue band light transmitted through the measured liquid S1. it can.

  Next, the function and effect of a method for measuring the concentration of dissolved matter in a sample using the concentration measuring device 1 (hereinafter referred to as a concentration measuring method) will be described.

  In this concentration measurement method, the light-receiving body 4 is obtained by substantially dividing the wavelength band of light in the visible light range out of the light from the light-emitting body 3 that has passed through the liquid S1 to be measured. Light and blue band light are received and the intensity of each light is measured, thereby calculating the absorbance (or transmittance) of these color band lights. For this reason, in this concentration measurement method, no matter what lysate concentration is measured (for example, the color of the liquid S1 to be measured is any color or the color of the liquid S1 to be measured is Even if the density changes from a specific color to another color), a simple pair of light emitters 3 and light receivers 4 is sufficient. For example, as in the past, light emission with different wavelengths for each type of dissolved material. It is not necessary to use multiple sets of light emitters and light receivers when using a body or measuring the concentration of a lysate that changes color depending on the concentration, so that measurement costs can be reduced and the measuring device can be downsized. . For this reason, the mutual interference of the light which arises when using several light-emitting body simultaneously is not produced.

  Further, in this concentration measurement method, the light emitter 3 only needs to emit light including a visible light range, and it is not necessary to emit only light of a specific wavelength unlike the conventional one. It is difficult to be affected by variations in wavelength depending on product lots or changes in output over time, and this illuminant 3 can always be used for highly accurate measurements.

  Further, in this concentration measurement method, even if dirt or bubbles adhere to the light emitting / receiving portions of the light emitting body 3 or the light receiving body 4 to cause a light attenuation effect, the light emitting body (specification used in the conventional measuring method) It emits light of a wavelength) and is less susceptible to the influence of a light receiving body and does not cause trouble in measurement.

  In addition, when the color of the liquid S1 to be measured indicates only a red complementary color, a green complementary color, or a blue complementary color, the light receiving body 4 includes a photodiode D1 and an G filter each including an R filter. Any one of the photodiode D2 and the photodiode D3 including the B filter may be provided. In addition, when the color of the liquid S1 to be measured indicates an intermediate color between the three complementary colors, the photoreceptor 4 includes a photodiode D1 having an R filter, a photodiode D2 having a G filter, and a photodiode D3 having a B filter. It is sufficient to have any two of them.

  Furthermore, the photoreceptor 4 may be one using a CCD sensor or a CMOS sensor in addition to the RGB color sensor.

  Further, the above description regarding the concentration measuring method applies to the concentration measuring apparatus 1 as well.

  Further, as shown in FIG. 4, the liquid discharge line 6 is provided with a constant flow valve 62 and an electromagnetic valve 63 together with a strainer 61, and these are used to supply the liquid S1 to be measured to the measurement cell 2. Also good. Also, as shown in FIG. 4, a reagent supply line 9 is provided in 2 that can be measured, and the reagent in the tank 91 is directly supplied to the measurement cell 2 using the pump 90, and this reagent and liquid supply line 6 is supplied. The sample to be measured may be stirred in the measurement cell 2, and the measurement liquid S1 colored by the reagent may be produced in the measurement cell 2.

  In the above description, the liquid S1 to be measured is colored according to the dissolved substance concentration by adding a reagent to the sample. However, the sample itself is colored by the dissolved substance, and the density of the sample is determined by the concentration of the dissolved substance. In this case, the sample itself becomes the liquid S1 to be measured, and it is not necessary to add a reagent.

Next, the density measurement result by this density measurement method and the density measurement result by the JIS analysis method using a spectrophotometer will be compared and described while showing specific measurement data. Note that the measurement data obtained by this concentration measurement method is that light from the light emitter 3 that has passed through the liquid S1 to be measured is reflected by a reflecting plate placed opposite the light emitter 3, and the reflected light is reflected on the light to be measured. The light is transmitted through the measuring liquid S1 again and then received by the light receiving body 4 on the light emitting body 3 side. However, since the data relating to the light transmission can be almost identical, the measurement data in this case Will be described as measurement data by this concentration measurement method.
[Measurement Example 1]
First, the measurement of phosphate ion concentration will be described.

  A sample prepared by dissolving 3 sodium phosphate in pure water and adjusting the phosphate ion concentration to 0, 1, 2, 3, 4, 5 mg / L was prepared. To this, molybdenum according to JIS 8224-1993 was prepared. Based on the blue (ascorbic acid reduction) absorptiometric method (the wavelength of the light source is 880 nm), a reagent is added, and this sample is colored to produce the measurement liquid S1. Subsequently, the absorbance of these liquids S1 to be measured was measured using a spectrophotometer (UV1700, manufactured by Shimadzu Corporation) based on JIS 8224-1993. In this case, as a reference, absorbance was also measured using light sources with wavelengths of 630 nm, 550 nm, and 470 nm corresponding to red light, green light, and blue light. Subsequently, the absorbance of these liquids S1 to be measured was measured for red band light, green band light, and blue band light by this concentration measurement method using the concentration measuring apparatus 1. In this case, the intensity of each band light when the light from the light emitter 3 when the transparent sample before addition of the reagent is passed through the measurement cell 2 is received by the light receiver 4 is assumed to have a transmittance of 100%. . The measurement regarding the transmittance of 100% may be performed only once for a plurality of concentrations of the lysate. However, in order to increase the measurement accuracy, it is preferable to perform the measurement for each concentration.

  FIG. 5 (a) shows the absorbance values for various phosphate ion concentrations measured by this concentration measurement method and a method using a spectrophotometer. FIG. 5B shows the absorbance and phosphoric acid for red band light measured by this concentration measurement method and light having wavelengths of 880 nm and 630 nm measured by a method using a spectrophotometer. The relationship with the ion concentration is shown in a graph.

  From FIG. 5A, in the method using the spectrophotometer, when the wavelength of the light source is 880 nm (infrared light), the change in absorbance is large with respect to the phosphate ion concentration, and one specific light source is used. When selecting, it turns out that it is preferable to select the light source whose wavelength is 880 nm. Further, from FIG. 5 (a), in this concentration measurement method, the change in absorbance with respect to the phosphate ion concentration is larger in the case of red band light than in the case of green band light or blue band light, and thus the specific measurement. It is understood that it is preferable to select red band light when selecting one of the band lights. Further, from FIG. 5B, even in the case of red band light or light having a wavelength of 630 nm, the change in absorbance with respect to the phosphate ion concentration is large (the slope of the graph is large), so even when these lights are used. It can be seen that there is no hindrance in the measurement of the phosphate ion concentration. In the case of red band light, it is considered that the change in absorbance is larger than that in the case of light having a wavelength of 630 nm due to the influence of light in a wavelength region longer than 630 nm.

[Measurement Example 2]
Next, measurement of calcium hardness will be described. The sample in which the calcium hardness component is dissolved develops color when a reagent (triethanolamine 80%, ethanol 20%, Eriochrome Black T 0.025%) is added, but the color is a hardness value (concentration). It depends on.

  Prepare samples prepared by dissolving calcium chloride dihydrate in pure water and adjusting the calcium hardness to 0, 2, 5, 10 mg CaCO3 / L, add 4 mg of reagent to 50 mg of these samples, and stir well -Color development is performed to make the liquid S1 to be measured. Next, the absorbance of these liquids S1 to be measured was measured with a spectrophotometer (UV1700 manufactured by Shimadzu Corporation) using light with wavelengths of 630 nm, 550 nm, and 470 nm. Subsequently, the absorbance of these liquids S1 to be measured was measured for red band light, green band light, and blue band light by this concentration measurement method using the concentration measuring apparatus 1. In this case, the light intensity of each band when the light from the light emitter 3 when the transparent sample before addition of the reagent was passed through the measurement cell 2 was received by the light receiver 4 was assumed to have a transmittance of 100%.

    FIG. 6A shows the absorbance for various calcium hardnesses measured by this concentration measurement method and a method using a spectrophotometer, and FIG. 6B shows the concentration measurement method and the spectrophotometer. The graph shows the relationship between the absorbance measured by a method using a meter and the calcium hardness.

  From FIG. 6B, in the method using a spectrophotometer, in the case of light having a wavelength of 630 nm, the graph N1 changes so that the absorbance decreases with an increase in calcium hardness, and in the case of light having a wavelength of 550 nm. When the graph N2 changes so that the absorbance increases as the calcium hardness increases, and the calcium hardness exceeds a certain value, the graph N1 and the graph N2 intersect, and the absorbance of light having a wavelength of 550 nm is 630 nm. It turns out that it becomes larger than the light absorbency of. On the other hand, even in this concentration measurement method, regarding the absorbance graph, the graph M1 in the case of red band light can be brought closer to the graph N1, and the graph M2 in the case of green band light can be brought closer to the graph N2. If a calibration graph of absorbance relating to calcium hardness is prepared for red band light and green band light, the calcium concentration can be easily calculated from the respective absorbances of red band light and green band light. In addition, if a calibration graph showing the relationship between the value of (absorbance of red band light / absorbance of green band light) and calcium hardness is prepared, the ratio value of the absorbance of these band lights is obtained. The calcium hardness can be calculated easily.

Embodiment 2. FIG.
By the way, the concentration measuring apparatus 1 can also be used when detecting the color tone (hue) of the liquid S2 to be detected. Therefore, a method for detecting a color tone of the liquid S2 to be detected (hereinafter referred to as a color tone detection method) using the same color tone detection device 1A as the concentration measuring device 1 will be described below. In the color tone detection apparatus 1A, parts having the same functions as those of the density measurement apparatus 1 are denoted by the same reference numerals, and description thereof is omitted.

First, the difference between the color tone detection device 1A and the density measurement device 1 will be described.
Since the color tone detecting device 1A needs to detect various color tones, the photoreceptor 4 includes, for example, a photodiode D1 having an R filter, a photodiode D2 having a G filter, and a B filter. It is necessary to have three photodiodes D3. Further, the arithmetic processing unit 8A of the color tone detection device 1A has a table showing the relationship between the absorbance and transmittance of the red band light, the green band light, and the blue band light with respect to various color tones of the liquid S2 to be detected. Based on the light intensity signals of the red band light, the green band light, and the blue band light output from the light receiving body 4 and calculating the time average light intensity or absorbing the specific color Using the intensity of the emitted light and the intensity of the light without absorption (which may store a representative value), the absorbance and transmittance of each of the red band light, the green band light, and the blue band light are calculated. A calculation unit that calculates, a selection unit that selects the color tone of the liquid S2 to be detected from the storage unit based on the absorbance and transmittance of the red band light, the green band light, and the blue band light; and the selected color tone And a display unit for displaying color numbers and color samples.

  In addition, when the color tone detection device 1A is specialized in detecting a specific color tone, the arithmetic processing device 8A does not calculate the absorbance and the transmittance for the red band light, the green band light, and the blue band light, respectively. A certain calculation (for example, red band light intensity / green band light intensity, red band light intensity / blue band light intensity, etc.) is performed for each light intensity, and the detected liquid has a specific color tone based on the calculated values. It may also be possible to detect whether or not

  In this color tone detection method, after passing through the measurement cell 2, the light to be detected S2 that is stationary in the measurement cell 2 is made to pass through the light from the light emitter 3, and a part of the light passes through the liquid S2 to be detected. The absorbed transmitted light is received by the photoreceptor 4 to detect the color tone of the liquid S2 to be detected. In this case, the light receiver 4 is obtained by substantially dividing the wavelength band of light in the visible light region out of the light from the light emitter 3 that has passed through the liquid S1 to be measured. Band light is received and the intensity of each light is measured. For this reason, in this color tone detection method, no matter what color tone is detected or when a color tone that changes with time is detected, a simple set of light emitter 3 and light receiver 4 is sufficient. Since it is not necessary to use a plurality of light emitters having different wavelengths depending on the number of color tones, the detection cost can be reduced and the size of the detection apparatus can be reduced. For this reason, the mutual interference of the light which arises when using several light-emitting body simultaneously is not produced.

  Further, in this color tone detection method, the light emitter 3 only needs to emit light including a visible light range, and it is not necessary to emit only light of a specific wavelength. It is difficult to be affected by variations and changes in output with time, and this illuminant 3 can always be used for accurate measurement.

  Further, in this color tone detection method, even if dirt or bubbles adhere to the light emitting / receiving portions of the light emitting body 3 or the light receiving body 4 to cause a light attenuation effect, the light emitting body (specification used in the conventional measuring method) It emits light of a wavelength) and is less susceptible to the influence of a light receiving body and does not cause trouble in measurement.

  In addition, the above description regarding the color tone detection method is similarly applied to the color tone measuring apparatus 1A.

  The photoreceptor 4 may be one using a CCD sensor or a CMOS sensor in addition to the RGB color sensor, and the same effect can be obtained even if a color filter having a band other than red, blue, or green is used. Can be obtained.

  Furthermore, this color tone detection method detects leaks of colored liquids (for example, antifreeze liquid) to the process side, manages wastewater treatment water from dyeing factories, food factories (such as tea), plating factories, painting booths, and paper pulp. Good and bad during clarifier treatment (clarification treatment) of the smelt solution (green liquor) in the factory (if it is good, the supernatant liquid is green, but if it is poor (insufficient), the color changes from green to reddish Etc.) can be easily detected.

It is a figure which shows the whole structure of the density | concentration measuring apparatus which concerns on one embodiment of this invention. It is operation | movement explanatory drawing of a density | concentration measuring apparatus. It is an electrical wiring diagram in the input / output part. It is explanatory drawing of another density | concentration measuring apparatus. It is a figure which shows the measured value of the light absorbency with respect to the phosphate ion concentration when using the concentration measuring device and when using the spectrophotometer, and (a) summarizes the phosphate ion concentration and the absorbance value in a table. (B) is a graph showing the relationship between phosphate ion concentration and absorbance. It is a figure showing the measured value of absorbance with respect to calcium hardness when using a concentration measuring device and when using a spectrophotometer, (a) is a table summarizing the values of calcium hardness and absorbance, (B) is a graph showing the relationship between calcium hardness and absorbance. It is.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Density measuring device 1A Color tone measuring device 3 Light emitter 4 Light receiver F Filter
S1 liquid to be measured
S2 liquid to be detected

Claims (2)

And the measured fluid in the sample that are colored with test solution or lysates colored by the addition of a reagent to the sample, by transmitting light from the light emitters and no absorption of light transparent target solution, these The transmitted light with respect to the liquid to be measured, which is colored or colored, is calculated by receiving each of the transmitted light with a light receiving body placed facing the light emitter so as to sandwich the liquid to be measured. A lysate concentration measurement method for measuring a concentration of a specific lysate in the sample based on light absorbance ,
The light emitter emits light including a visible light region, and the light receiver is obtained by substantially dividing the light in the visible light region out of light from the light emitter transmitted through the liquid to be measured. Receiving either the light of the red region component, the light of the green region component, or the light of the blue region component, or each of the plurality of region components that is a combination thereof ,
And when the liquid to be measured changes the hue with a change in the concentration of the specific dissolved matter, the specific absorbance is calculated using the absorbance values calculated for the light of the plurality of region components. When calculating the concentration of the lysate and the liquid to be measured does not change the hue with a change in the concentration of the specific lysate, the absorbance value calculated for the light of one region component is calculated. A method for measuring a concentration of a lysate , wherein the concentration of the specific lysate is calculated . And the measured fluid in the sample that are colored with test solution or lysates colored by the addition of a reagent to the sample, by transmitting light from the light emitters and no absorption of light transparent target solution, these The transmitted light with respect to the liquid to be measured, which is colored or colored, is calculated by receiving each of the transmitted light with a light receiving body placed facing the light emitter so as to sandwich the liquid to be measured. A lysate concentration measuring device that measures the concentration of a specific lysate in the sample based on light absorbance ,
The light emitter emits light including a visible light region, and the light receiver is obtained by substantially dividing the light in the visible light region out of light from the light emitter transmitted through the liquid to be measured. , have a filter for transmitting light in the red region component, the green region component light, or blue region component or filter for transmitting any of the light, or light of a plurality of regions ingredient in combination with their respective And
And when the liquid to be measured changes the hue with a change in the concentration of the specific dissolved matter, the specific absorbance is calculated using the absorbance values calculated for the light of the plurality of region components. When calculating the concentration of the lysate and the liquid to be measured does not change the hue with a change in the concentration of the specific lysate, the absorbance value calculated for the light of one region component is calculated. A device for measuring the concentration of a lysate , comprising an arithmetic processing unit for calculating the concentration of the specific lysate .

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