JP5375681B2 - Electrophoresis cassette - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an electrophoretic cassette preventing that a gel enters outside unevenness to become difficult to remove when an electrophoretic cassette equipped with an outside space such as a buffer solution tank having a complicated shape or the like is filled with the gel and preventing the adhesion of the gel to the outside space of the cassette especially when the electrophoretic cassette is immersed in an immersion layer filled with a gel precursor solution and the gel precursor solution is introduced into the cassette from the opening part provided to the bottom of the cassette. <P>SOLUTION: The electrophoretic cassette for including an electrophoretic gel includes: an internal space for holding the electrophoretic gel; and the outside space to one side of the electrophoretic cassette, wherein the other side of the electrophoretic cassette opposed to one side of the electrophoretic cassette corresponds to the outside space and formed into a lid structure which can close the outside space of another electrophoretic cassette including the same structure. <P>COPYRIGHT: (C)2011,JPO&amp;INPIT

Description

  The present invention relates to an electrophoresis cassette used for analyzing a protein or the like by filling a gel, introducing a sample such as a protein into the gel and applying a voltage thereto.

  Electrophoresis is known as a method for separating genes and proteins. In particular, a method is generally used in which a separation sample is introduced into a gel medium containing an electrolyte, and a voltage is applied to both ends of the gel medium for separation.

  One widely used method for separating a protein mixture is a gel electrophoresis method called SDS-PAGE. The separated sample is coated with SDS (sodium dodecyl sulfate) to give a charge to the protein, introduced into a polyacrylamide gel, and moved through the gel by applying a voltage to the gel medium (electrophoresis). Since the distance of movement differs depending on the size of the protein, it is a method in which different types of proteins can be separated.

  In SDS-PAGE, after the separated sample (sample) is introduced into the gel medium, the cathode and one end of the gel medium, and the anode and the other end of the gel medium are brought into contact with each other via a buffer solution, and an electric current is applied between the two electrodes. This is done by flowing. When an electric current is started to flow through the gel medium, the protein starts to move in the gel from the cathode toward the anode, and the protein moves faster as its molecular weight is smaller.

  The gel plate used for SDS-PAGE is created by these members, and two flat supports (plates), a support frame (spacer) that is provided between them and creates a space for holding the gel. It consists of a gel medium filled in the internal space. In general, the experimenter himself poured a solution (gel precursor solution) for gel preparation into a flat glass plate (plate) and polymerized it. However, in recent years, there is an increasing demand for precast gel plates as ready-made products for convenience and reproducibility. The precast gel (plate) is also basically structured such that the gel medium is sandwiched between flat transparent substrates such as glass and resin, and one end and the other end of the gel medium are respectively connected during electrophoresis. Touch the buffer solution and apply voltage.

  Patent Document 1 describes an electrophoresis cassette (hereinafter simply referred to as a cassette) in which a buffer solution tank for electrophoresis is integrated with a precast gel plate. Unlike the conventional gel plate in which the gel is sandwiched between two flat plates, this electrophoresis cassette has a space (external space) that can hold a buffer solution for electrophoresis integrated with the cassette outside the cassette in advance. It is provided. This cassette is very convenient because electrophoresis is possible without preparing a buffer solution tank in addition to the cassette.

By the way, the (empty) electrophoresis cassette is used as an electrophoresis gel cassette (hereinafter sometimes simply referred to as a gel cassette) by filling the inner space with a (polyacrylamide) gel for electrophoresis. Conventionally, the method of pouring the gel precursor solution into each cassette from the upper cassette opening and waiting for it to solidify has been used for this filling.
However, in this method, one or more nozzles for supplying the gel precursor solution are required for filling one cassette, and the nozzles must be individually filled. Therefore, there is a variation in the quality such as gel filling amount and composition. It can happen. Moreover, there was a problem that it took time and was not suitable for mass processing.

In order to solve this problem, a method has been proposed in which a plurality of empty cassettes (gel plates) are entirely immersed in a gel precursor solution, and the inside of the cassette is filled with the gel precursor solution through an opening at the bottom of the cassette (patent) Reference 2). Since the opening at the bottom of the cassette is also an opening for the gel to come into contact with the buffer solution during electrophoresis, no special structure is required for the cassette itself.
According to this method, a plurality of gel cassettes for electrophoresis can be prepared at a time. Since it is only necessary to prepare an immersion tank of a size that can accommodate a plurality of cassettes, the facilities can be simplified, and if the immersion tank is enlarged, there is an advantage that it is suitable for mass processing. Moreover, there is an advantage that a large amount of gel cassettes for electrophoresis having the same quality can be obtained.

  However, in the method of filling a gel by immersing a plurality of cassettes in a gel precursor solution, heat is generated during gel curing, but there is a problem that this heat does not escape and the cassette and the immersion tank become hot. It was. Further, in the method of Patent Document 2, there is a space between the cassettes, but there is a problem that the cassettes are broken due to the removal of the gel sandwiched between the cassettes.

  Furthermore, the cassette provided with the external space is not suitable for the method of immersing the cassette described above in the immersion tank and filling the gel precursor solution from the bottom opening. This is because unnecessary gel adheres to the external space and is very difficult to remove. Therefore, for a cassette having a complicated shape with an external space, the gel filling method is the only way to fill from the upper opening as in the past.

JP 2007-64848 A Japanese Patent No. 3071927

  The object of the present invention is to prevent the gel from entering the external irregularities when the gel is filled into the electrophoresis cassette having a complicated external shape, for example, an external space such as a buffer solution tank. That is. In particular, when the electrophoresis cassette is immersed in an immersion layer filled with the gel precursor solution and the gel precursor solution is introduced into the cassette from the opening at the bottom of the cassette, the gel does not adhere to the external space of the cassette. An object is to provide a cassette for electrophoresis.

  Another object of the present invention is to provide a cassette having a structure in which the cassette does not become hot even in batch processing in which a plurality of cassettes are filled with a gel precursor solution at a time.

In order to solve the above problems, a first invention is an electrophoresis cassette for encapsulating an electrophoresis gel, wherein an internal space for holding the electrophoresis gel, and the electrophoresis A first opening and a second opening provided on one surface of the cassette and capable of contacting the internal space, and first and second external portions provided on one surface of the electrophoresis cassette and capable of holding a liquid The first opening is connected to the first external space, the second opening is connected to the second external space, and is not connected to the first and second external spaces, Another electrophoresis cassette having a third opening that can contact the inner space, the other surface of the electrophoresis cassette facing the one surface corresponds to the outer space, and has the same structure It is a lid structure that can block the external space of One is an electrophoresis cassette, characterized in that the a electrophoretic cassette can ventilation between the another internal space in which the electrophoresis cassette is provided in plugged external space of the electrophoresis cassette.

The second invention made to solve the above-mentioned problems is an electrophoresis cassette for enclosing an electrophoresis gel, the internal space for holding the electrophoresis gel, First and second openings provided on one surface of the electrophoresis cassette and capable of contacting the internal space, and first and second openings provided on one surface of the electrophoresis cassette and capable of holding a liquid Two external spaces, the first opening is connected to the first external space, the second opening is connected to the second external space, and is further connected to the first and second external spaces. The outer space of the electrophoresis cassette is provided with a third opening that can contact the inner space, and the outer space is arranged to face the outer space forming surface of the other electrophoresis cassette. By other said electro-swimming with the same structure A lid structure capable of closing the external space of the cassette, and when the external space of the other electrophoresis cassette is closed with the electrophoresis cassette, ventilation with the internal space of the electrophoresis cassette is provided. It is a cassette for electrophoresis characterized by being possible.

  According to the first and second inventions described above, a plurality of electrophoresis cassettes having the same structure are arranged and filled with the gel precursor solution even if the electrophoresis cassette has a complicated structure having an external space. Thus, the gel precursor solution does not enter the external space because the adjacent other electrophoresis cassette covers the external space of the electrophoresis cassette. Therefore, it is not necessary to remove the gel, and the gel precursor solution filling process can be performed on a plurality of cassettes at once (in a batch process). Further, since the plurality of cassettes are closely packed with the gel, it is not necessary to remove the gel sandwiched between the cassettes. And if a plurality of cassettes are processed at once, the heat generated during gelation becomes a problem, but according to the present invention, the external space provided in the cassette becomes a heat insulating layer, and the entire cassette becomes hot during gel curing. Can be suppressed.

  Furthermore, regarding the first invention and the second invention, a first opening and a second opening which are provided on one surface of the electrophoresis cassette and can contact the internal space, and one of the electrophoresis cassettes First and second external spaces that can hold liquid, and the first opening is connected to the first external space, and the second opening is connected to the second external space. And a third opening that is not connected to the first and second external spaces and is capable of contacting the internal space, and the other cassette for electrophoresis is provided in the electrophoresis cassette. When the external space is blocked, the gel cassette can be ventilated with the internal space of the electrophoresis cassette, so that the gel precursor is introduced into the internal space from the third opening. Filled with solution, inside said internal space Since the gas can be discharged, it can be performed without depositing the gel to the external space, the filling of the gel precursor solution to the cassette. In particular, even in the downward filling method in which the gel precursor solution is immersed in the gel precursor solution immersion tank together with the electrophoresis cassette and the gel precursor solution is filled from the opening below the planned position of the gel precursor solution filling, adhesion of the gel to the external space is prevented. be able to.

  Furthermore, by combining the first invention and the second invention, it is possible to cope with one or more electrophoresis cassettes that require a gel precursor solution filling process even when there are a plurality of or even number of cassettes for electrophoresis.

  As described above, by using the electrophoresis cassette of the present invention, it is possible to efficiently perform the downward filling method suitable for mass processing even in the electrophoresis cassette having a functional external space, and at the time of gel curing. The effect of heat generation can be minimized.

(A) It is the figure which looked at the cassette for electrophoresis of this invention from the external space formation surface. (B) It is sectional drawing in the AB line | wire of Fig.1 (a). (C) It is the side view which looked at Fig.1 (a) from the C direction. (D) It is the side view which looked at Fig.1 (a) from the D direction. It is sectional drawing of the side which shows some examples of the cassette for electrophoresis of this invention. It is explanatory drawing which shows a mode that several cassettes for electrophoresis of this invention were piled up. It is sectional drawing which shows the gel cassette for electrophoresis which filled the cassette for electrophoresis of this invention with the gel. It is a figure explaining an example with which the gel precursor solution is filled in the gel cassette for electrophoresis of this invention.

The present invention will be described with reference to FIGS.
The electrophoresis cassette of the present invention includes an internal space for holding the electrophoresis gel and an external space provided outside the electrophoresis cassette, and the electrophoresis cassette of the electrophoresis cassette facing the one surface is provided. The other surface corresponds to the external space and has a lid structure that can block the external space of another electrophoresis cassette having the same structure. Alternatively, when the external space is arranged to face the external space forming surface of the other electrophoresis cassette, the external space of the electrophoresis cassette can be replaced by another electrophoresis cassette having the same structure. It has a lid structure that can block the external space.
That is, as shown in FIGS. 3 (a), (b), (c), and (d), when a plurality of cassettes are arranged in close contact, one cassette can serve as a lid for another cassette. Structure.

  The external space is a state in which the outside of the cassette is not flat, and irregularities for adding functions are provided outside the cassette, thereby forming a space. For gel removal, there can be mentioned a mechanism for sending outside air into the cassette (indentation and air holes) and a process for improving finger catching and easy handling (irregularities and anti-slip). In particular, as shown in FIGS. 1 and 2, a space (buffer solution tank) for holding a buffer solution can be mentioned.

The electrophoresis cassette has at least two openings. A first opening for touching the buffer solution connected to the first electrode and a second opening for touching the buffer solution connected to the second electrode.
The external space is divided into at least two. A first external space serving as a buffer solution tank connected to the first electrode, and a second external space serving as a buffer solution tank connected to the second electrode.

  When the electrophoresis cassette is filled with gel, as a first method, either one of the openings is sealed, and the cassette is placed so that the sealed opening is below the other opening. There is a method of arranging and filling the gel precursor solution from the other opening (that is, from the opening above the planned filling position of the gel precursor solution) (upper filling method). In the case of the electrophoresis cassette of the present invention, when the gel precursor solution is filled in one or a plurality of cassettes, even if the gel precursor solution adheres to the outside of the cassette, the complicated irregularities outside the cassette may be other cassettes. Since it is sealed by, unnecessary gel can be easily removed.

  Alternatively, the first external space and the second external space are all sealed with another cassette, that is, the first opening and the second opening are also sealed, and the third opening is provided, from which the gel precursor solution is poured. A method is also conceivable.

As a second method of filling the gel into the electrophoresis cassette, there is a method of filling the gel precursor solution from an opening disposed below the planned filling position of the gel precursor solution (lower filling method).
If the cassette does not have an external space, as described in Patent Document 2, one opening is disposed downward and the other opening is disposed upward, and the entire cassette is immersed in the gel precursor solution. Thus, the gel precursor solution can be filled into the cassette from the lower opening. However, in the cassette of the present invention, the first opening and the second opening both connect the internal space and the external space, so that if the conventional method is applied as it is, the external space located below becomes lower. Until the gel is filled.

  Therefore, in order to apply the downward filling method to the cassette having the external space, a third opening that can introduce the gel precursor solution into the internal space is required in addition to the first and second openings. become. The third opening can contact the internal space but is not connected to the external space. One or more third openings may be provided.

  Assuming that the gel precursor solution is filled with the first opening upward and the second opening downward, at least one or more third openings are below the position that requires filling of the gel precursor solution. Is preferred. If it is this position, the filling amount of a gel precursor solution can be adjusted with the water level of a gel filling tank (immersing the cassette for electrophoresis), and it is also possible to reduce the filling amount of a gel precursor solution. When the position of the third opening is above the planned filling position of the gel precursor solution, the gel precursor solution can be filled, but cannot be discarded when it is filled too much.

  Thus, when filling the gel precursor solution from the lower opening, an opening for connecting the internal space and the outside is required above the planned filling position of the gel precursor solution in order to remove air. This opening may be the first opening or the other opening when the gel precursor solution is filled with the first opening as the upper side and the second opening as the lower side. When the first opening is used as an opening for venting, the first opening and the first external space connected to the first opening are not completely sealed, that is, the inside Allow ventilation between the space and the outside.

Specifically, as shown in FIG. 1, a notch can be provided in the partition that partitions the external space so as to function as a vent hole. The ventilation hole 7a shown in FIG. 1 is a notch provided for exhausting the internal space. The vent holes 7b and 7c in FIG. 1 and FIG. 2 (b) are trapped in the gas when the external space is sealed by another electrophoresis cassette as a lid. In some cases, heat is generated, which may increase the pressure in the external space. In that case, the vent hole is provided so that the increased pressure can be released.
The shape of the cutout may be a semicircular shape or a through hole as long as the function of the external space is not impaired, and may be plural or single as long as the ventilation function can be maintained.

In addition, a step or depression can be provided on the other surface that serves as a lid, and a structure that can ventilate the internal space when the surface provided with the external space is closed can be formed (FIG. 2 ( c)).
According to the electrophoresis cassette 10c, when the first external space and the second external space are used as the buffer solution layer, the partition for partitioning the external space has no notch, which hinders the retention of the solution. It is preferable without any problem.
Such a vent must be provided at a position where it does not contact the gel precursor solution. In particular, the air holes 7a and 7d provided in the partition that separates the external space and the outside, not the partition that separates the external spaces from each other, are designed to be located above the planned filling position of the gel precursor solution. .

  For example, in the electrophoresis cassettes 10a to 10c shown in FIGS. 3A to 3D, a plurality of electrophoresis cassettes having the same structure are arranged adjacent to each other, and the second external space 3 is arranged by the adjacent electrophoresis cassettes. Are covered, but the first outer space 2 is not completely sealed. An example in which the gel is filled in the internal space 1 of the electrophoresis cassettes 10a to 10d by the downward filling method will be described by taking the electrophoresis cassette 10b as an example.

  As shown in FIG. 5, the gel is such that the first opening 4 of the gel cassette for electrophoresis is upward, the second opening 5 is downward, and the third opening 6 is below the first opening 4. Immerse in the precursor solution immersion bath 11. At this time, the electrophoresis cassette 10b is arranged in the same direction or facing the outer space forming surfaces, and the gel precursor solution that becomes the gel is sealed in the inner space with the outer space sealed by the adjacent cassette. Filled.

  As the gel precursor solution 12 is filled in the gel precursor solution immersion tank, the gel precursor solution 12 is also filled into the internal space 1 from the third opening 6 (FIG. 5). At this time, since the first external space 2 is not completely sealed, the gas in the internal space 1 is discharged to the outside through the first opening 4 and the vent hole 7a. In addition, since the vent hole 7 a provided in the partition that separates the external space and the outside is above the water surface of the gel precursor solution 12, the gel precursor solution 12 does not enter the first external space 2.

  As shown in FIG. 5, when electrophoresis cassettes having a plurality of external spaces are arranged in the same direction or so that the external spaces oppose each other and the gel precursor solution is filled into the internal spaces by the lower filling method, The cassettes are brought into close contact with each other so that they are not separated during the filling of the gel precursor solution. The cassette itself may be provided with irregularities so as to keep close contact with each other, but it is easier to divide the whole after filling and curing the gel precursor solution if the whole is bound without processing to the cassette situation preferable.

If multiple cassettes are arranged in the same direction and are not arranged so that the external space is opposed, the gel will enter the external space of the cassette placed at the end if it is left as it is, so seal the external space. In addition, a sealing material is arranged so that the internal space can be exhausted.
In this case, the sealing material may be a simple resin plate or a metal plate as long as it has a size capable of closing the external space. Moreover, when a sealing material is not affixed on a cassette, the base material with moderate thickness is preferable.

  Since the adjacent cassettes are pressed against the external space by binding the cassettes together, the gel precursor solution hardly flows from the outside to the external space during the filling of the gel precursor solution, while FIG. c) and the structure of the electrophoresis cassettes 10a and 10c shown in FIGS. 3 (a) and 3 (c), there is no escape place even when the gas sealed in the external space expands. There is a possibility that the gas breaks the gel by breaking the sealing material of the opening. In that case, vents 7b and 7c such as notches may be provided in the unevenness that separates the external spaces so that the gas in the external space can be discharged. At this time, care must be taken not to impair the function of the external space (especially as a buffer solution tank).

  When a plurality of third openings are provided, providing the same on the same surface of the electrophoresis cassette makes it easy to remove the gel from the cassette after the gel is cured. By providing a plurality of third openings on different surfaces of the electrophoresis cassette, the composition of the gel precursor solution in the cassette can be easily homogenized, and a high-quality electrophoresis gel cassette can be manufactured.

  When the gel is made into multiple layers, in the upper filling method, after the lower layer gel precursor solution is cured, a necessary amount of the upper layer gel precursor solution is poured and cured. In the lower filling method, the upper layer gel precursor solution is filled to a required amount, and the lower layer gel precursor solution is gently filled before the gel precursor solution is cured. At this time, by increasing the specific gravity of the lower layer gel precursor solution compared to the upper layer gel precursor solution, the multilayer structure can be filled without breaking the layer structure even before curing. At this time, the formation position of the third opening used for filling the gel precursor solution is preferably lower when the interface between the gel and the outside air is set upward, and the amount of the gel precursor solution in the upper layer is adjusted, In order not to break the layer structure of the upper layer gel precursor solution, the position is preferably lower than the thickness of the upper layer gel precursor solution.

  When filling a gel having a plurality of compositions, it may be a method of filling a gel precursor solution having a uniform composition and switching it halfway, or a method of filling while changing the concentration. For example, when filling a polyacrylamide gel having a concentration gradient, it is possible to prepare a high-concentration acrylamide gel precursor solution and a low-concentration acrylamide gel precursor solution separately and fill them while changing the mixing ratio over time. it can. Also at this time, a substance having a high specific gravity such as glycerin can be added to one of the solutions to fill the solution with a specific gravity difference.

The gel precursor solution may be filled into the cassette of the present invention by attaching a comb or the like to the first opening to create a sample slot.
After filling the cassette with the gel precursor solution, water or an organic solvent may be overlaid at the interface of the gel precursor solution to prevent contact between the gel precursor solution and air. At this time, it may be poured into the cassette one by one from the opening opened above the cassette. For example, in the lower filling method shown in FIG. 3, a solvent having a lighter specific gravity than the gel precursor solution is used as the gel precursor solution packed layer. It is also possible to flow into the cassette through the vents and openings provided in the electrophoresis cassette for exhausting the internal space. Here, since the solvent does not harden, it can be easily removed after the gel is hardened to perform the next step (layering or analysis of other gels, etc.).

  The gel precursor solution filled in the internal space of the cassette as described above is allowed to stand for a sufficient time until polymerization proceeds and gelation is performed, and then the cassette is taken out from the gel precursor solution immersion tank. Thereafter, the third opening is sealed with a film or a resin base material.

  A well-known gel precursor solution can be used to fill the internal space. For example, the acrylamide gel preparation solution described in the electrophoresis manual can be used. Acrylamide monomer. Examples thereof include a monomer solution composed of a crosslinking agent such as N, N'-methylenebisacrylamide, a pH buffering agent, and a modifying agent such as SDS (sodium dodecyl sulfate), if necessary, with a polymerization initiator added thereto.

  These gel precursor solutions generate heat during gelation and can often interfere with gelation, and this problem is particularly noticeable in the case of the lower filling method where curing is performed with the gel surrounded. However, when the electrophoresis cassette of the present invention is used, heat generation is gentle because the external space moderates heat conduction. In addition, when a plurality of cassettes are stacked and filled with a gel precursor solution at a time, the heat generated from the gel precursor solution of the adjacent cassette is accumulated and the same problem occurs. However, in the electrophoresis cassette of the present invention, the cassette This is unlikely to occur because an external space is sandwiched between the cassette and the cassette.

  When the gel precursor solution is filled into the internal space, the opening that connects the internal space and the external space and that is positioned below the planned filling position of the gel precursor solution is the gel precursor solution from the internal space to the external space. It must be sealed to prevent inflow. Furthermore, when the external space connected to the internal space by the opening is used as a buffer solution tank for electrophoresis, the sealing material that seals the opening must be removable from the external space side. .

  As such a sealing material for openings, any base material that can prevent the gel precursor solution from entering the external space may be used. For example, there can be mentioned a plug-like one in which a film or a resin plate is attached and a resin having appropriate elasticity and chemical resistance is fitted in accordance with the shape of the opening. If it is resin with moderate elasticity, it can stick to a cassette by pressing against the opening and can serve as a sealing material. Moreover, the same thing as the sealing material in the case of sealing external space separately can also be used.

  Note that, here, the electrophoresis cassette 10b provided with the vent holes 7b and 7c in the partition that separates the external spaces in addition to the partition that separates the external space from the external space has been described as an example, but only the vent hole 7a is provided. The electrophoresis cassette 10a and the electrophoresis cassette 10c having the vent hole 7d by providing a step or a depression on the other surface opposite to the surface provided with the external space and the first opening and the second opening are also used. Similarly, the gel precursor solution can be filled by a downward filling method. In addition, filling can be performed by combining electrophoresis cassettes having different structures.

As described above, according to the present invention, it is possible to prevent the gel from entering the external space when the cassette is filled with the gel. In particular, when a plurality of cassettes are filled with a gel, the gel can be filled particularly efficiently.
Also, in the downward filling method where the gel precursor solution is immersed in the gel precursor solution immersion tank together with the electrophoresis cassette and the gel precursor solution is filled from the opening below the planned position of the gel precursor solution filling, adhesion of the gel to the external space is prevented. And the influence of heat generation during gel curing can be minimized. Moreover, the downward filling method suitable for mass processing can be performed efficiently.

DESCRIPTION OF SYMBOLS 1 Internal space 2 1st exterior space 3 2nd exterior space 4 1st opening part 5 2nd opening part 6 3rd opening part 7a, 7b, 7c, 7d Air vent 8 Sealing material 9 Gel 10 Cassette 11 for electrophoresis Gel precursor solution immersion bath 12 Gel precursor solution 13 Travel direction 14 of gel precursor solution Vent

Claims (2)

An electrophoresis cassette for encapsulating an electrophoresis gel,
An internal space for holding the electrophoresis gel;
A first opening provided on one surface of the electrophoresis cassette and capable of contacting the internal space; a second opening;
Provided on one surface of the electrophoresis cassette, comprising first and second external spaces capable of holding a liquid;
The first opening is connected to the first external space, the second opening is connected to the second external space, and
Furthermore, the first and second external spaces are not connected, and a third opening that can contact the internal space is provided,
The other surface of the electrophoresis cassette facing the one surface corresponds to the outer space, and is a lid structure capable of closing the outer space of another electrophoresis cassette having the same structure, and the An electrophoresis cassette characterized in that, when an external space of the other electrophoresis cassette is blocked by the electrophoresis cassette, ventilation with the internal space of the electrophoresis cassette is possible . An electrophoresis cassette for encapsulating an electrophoresis gel,
An internal space for holding the electrophoresis gel;
A first opening provided on one surface of the electrophoresis cassette and capable of contacting the internal space; a second opening;
Provided on one surface of the electrophoresis cassette, comprising first and second external spaces capable of holding a liquid;
The first opening is connected to the first external space, the second opening is connected to the second external space, and
Furthermore, the first and second external spaces are not connected, and a third opening that can contact the internal space is provided,
When the external space is arranged facing the external space forming surface of the other electrophoresis cassette, the external space of the other electrophoresis cassette having the same structure depends on the external space of the electrophoresis cassette. A lid structure that can block the outer space of the other electrophoresis cassette with the electrophoresis cassette, and can be ventilated with the inner space of the electrophoresis cassette. A cassette for electrophoresis.

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