JP5317794B2 - Benikouji pigment preparation and method for producing the same - Google Patents

Description

  The present invention relates to a beige mushroom pigment preparation with improved light stability and a method for producing the same.

  Benikouji pigment has been widely used as a natural colorant for foods and cosmetics. However, because Benikouji dyes are not stable to light, foods that are colored using Benikouji dyes are exposed to light such as fluorescent light or sunlight, and fading occurs in a very short time. There was a disadvantage of damaging.

  In order to improve the stability of the beech mushroom dye by this light irradiation, a method of adding a sesamorin derivative to the red yeast rice dye (Patent Document 1), a method of sealing and storing the bemoji dye together with an oxygen scavenger (Patent Document 2), A method of adding tryptophan or the like to a pigment (Patent Document 3), an anti-fading agent for a pigment containing extracted tocopherol and ferulic acid (Patent Document 4) has been studied, but a method that is still satisfactory can be found. Not.

JP-A-10-110109 JP 2002-253168 A Japanese Patent Laid-Open No. 2003-191 JP 2004-222649 A

  An object of the present invention is to provide a beige mushroom pigment preparation with improved light stability, which is used for coloring processed foods and the like.

  As a result of intensive studies to solve the above-mentioned problems, the present inventor obtained by emulsifying an aqueous phase containing Benikouji pigment and an oil phase containing polyglycerin condensed ricinoleic acid ester and edible fats and oils having specific physical properties. It was found that foods colored by adding a beige mushroom pigment preparation obtained by drying a water-in-oil emulsion composition obtained under reduced pressure have improved stability to light. It came to an eggplant.

That is, this invention consists of following (1)-(3).
(1) (a) Benikouji pigment, (b) Benikouji pigment preparation containing polyglycerin-condensed ricinoleic acid ester having an emulsified phase ratio of 81 to 89% in the following emulsification test and (c) edible oil and fat.
<Emulsifying power test>
1) In a 500 ml beaker, 200 g of rapeseed white squeezed oil (manufactured by Borso Oil & Fats Co., Ltd.) and 0.80 g of polyglycerin condensed ricinoleate are mixed and heated to 60 ° C. to obtain an oil phase.
2) The oil phase of 1) was stirred at 3000 rpm using an emulsifier (model: TK homomixer MARK II 2.5 type; manufactured by Primix Co., Ltd.) in which the gap between the mixer part and the baffle plate was fixed at 2.5 cm. Then, 200 g of purified water heated to 60 ° C. was added to the oil phase over 90 seconds.
3) Furthermore, stirring at 10000 rpm for 3 minutes while adjusting the temperature to 60 ° C., a water-in-oil emulsion is obtained.
4) Add 100 ml of the water-in-oil emulsion obtained in 3) to a 100 ml stoppered graduated cylinder and store it in a thermostat at 37 ° C. for 120 hours. After storage, the volume (ml) of the emulsified phase is measured for the contents of the graduated cylinder separated into an oil phase, an emulsified phase and an aqueous phase, and the proportion (%) of the emulsified phase is calculated based on the following formula.

(2) The beech mushroom pigment preparation according to (1), wherein the beco mushroom pigment is a bem moji pigment powder, and the partition coefficient is -1.2 to -0.1 when the following hydrophilicity discrimination test is performed on the bem moji pigment powder.
<Hydrophilicity discrimination test>
1) Put 0.002 g of Benikouji pigment powder (converted to a color value of 1000) into a 50 ml capacitative colorimetric tube, add 15 ml of ion exchange water, and mix well to obtain a solution.
2) Add 15 ml of 1-octanol (special grade reagent; manufactured by Wako Pure Chemical Industries, Ltd.) to the solution of 1) and shake for 5 minutes while rotating the colorimetric tube 180 degrees up and down (rotation number 20 times / Min).
3) After shaking, the contents of the colorimetric tube are placed in a 50 ml centrifuge bottle and centrifuged for 15 minutes at a centrifugal acceleration of 12000 × g using a centrifuge.
4) After centrifugation, the 1-octanol phase and the aqueous phase in the centrifuge bottle are collected, and the absorbance at the visible maximum absorption wavelength and the absorbance at 660 nm are measured for each of these phases using a spectrophotometer.
5) The absorbance at the visible maximum absorption wavelength of the 1-octanol phase is O1, the absorbance at 660 nm of the 1-octanol phase is O2, the absorbance at 660 nm of the aqueous phase is W1, and the absorbance at 660 nm of the aqueous phase is W2. Calculate the distribution coefficient based on the formula.

(3) an oil containing (a) an aqueous phase containing a beech powder, (b) a polyglycerin condensed ricinoleic acid ester in which the proportion of the emulsified phase in the emulsifying ability test is 81 to 89%, and (c) an edible oil / fat A method for producing a beige mushroom pigment preparation, comprising: a step of emulsifying a phase to obtain a water-in-oil emulsion composition; and a step of drying the water-in-oil emulsion composition under reduced pressure.

  The stability to light of foods such as sea urchins colored with the beech powder dye preparation of the present invention is sufficiently satisfactory in practice.

Benikouji pigment preparation The Bencouge pigment formulation of the present invention comprises (a) Bencouge pigment, (b) polyglycerin condensed ricinoleic acid ester in which the proportion of the emulsified phase in the emulsifying ability test is 81 to 89%, and (c) edible oil and fat. To do.

Benikouji pigment The Benikouji pigment used in the present invention is a water-soluble red pigment obtained by extraction from a culture product of Beniculomycetes (i.e., filamentous fungi of the genus Monascus), and the main components thereof are monascorbrins and anchors. Such as flavins.

  There are no particular restrictions on the form of the beech powder used for the production of the bemoji dye preparation of the present invention, and examples include water solution / alcohol solution of bemoji dye and powder of bemoji dye, preferably bemoji dye powder, and an outline of the production method thereof Is as follows.

  Using a water / alcohol mixed solution as an extraction solvent, Benikouji pigment is extracted from the culture product of B. niger, and a water solution of alcohol with a beetle pigment / alcohol solution is prepared. Next, a method of obtaining the powdered beech powder by spraying the mixed solution with the excipient (for example, dextrin) and the mixture and spray-drying the mixed solution by a conventional method, or the solution of the colored water / alcohol solution Concentrate and obtain a beige powder pigment by a method such as vacuum lyophilization.

The beech mushroom dye used in the present invention is preferably a bemoji dye powder having a distribution coefficient of -2.0 to 1.5 when the following hydrophilicity discrimination test is performed, and the distribution coefficient is -1.5 to 0.7. Are more preferable, and a powdered beech powder having a partition coefficient of -1.2 to -0.1 is particularly preferable. When the distribution coefficient is in the range of -1.2 to -0.1, the stability of the food colored with the beige mushroom pigment preparation of the present invention to light is further improved.
<Hydrophilicity discrimination test>
1) Put 0.002 g of Benikouji pigment powder (converted to a color value of 1000) into a 50 ml capacitative colorimetric tube, add 15 ml of ion exchange water, and mix well to obtain a solution.
2) Add 15 ml of 1-octanol (special grade reagent; manufactured by Wako Pure Chemical Industries, Ltd.) to the solution of 1) and shake for 5 minutes while rotating the colorimetric tube 180 degrees up and down (rotation number 20 times / Min).
3) After shaking, the contents of the colorimetric tube are placed in a 50 ml centrifuge bottle and centrifuged for 15 minutes at a centrifugal acceleration of 12000 × g using a centrifuge.
4) After centrifugation, the 1-octanol phase and the aqueous phase in the centrifuge bottle are collected, and the absorbance at the visible maximum absorption wavelength and the absorbance at 660 nm are measured for each of these phases using a spectrophotometer.
5) The absorbance at the visible maximum absorption wavelength of the 1-octanol phase is O1, the absorbance at 660 nm of the 1-octanol phase is O2, the absorbance at 660 nm of the aqueous phase is W1, and the absorbance at 660 nm of the aqueous phase is W2. Calculate the distribution coefficient based on the formula.

  In addition, when the form of the beige mushroom dye used in the production of the bemoji dye preparation of the present invention is something other than the bemoji dye powder (for example, bemoji dye water / alcohol solution), the bemoji dye is pulverized by a method known per se. In this way, after preparing a beige mushroom dye powder, the hydrophilicity discrimination test can be performed on the bemoji dye powder.

  Here, the distribution coefficient in the hydrophilicity discrimination test is an index indicating the degree of hydrophilicity of the beige mushroom dye. The larger the value of this distribution coefficient, the lower the hydrophilicity of the beige mushroom dye.

  Examples of Benikouji pigment powder having a distribution coefficient in the hydrophilicity discrimination test of −2.0 to 1.5 were powdered, for example, Riquecolor R-30 (trade name; Bencouge pigment water / alcohol solution; manufactured by Riken Vitamin Co., Ltd.). (Distribution coefficient 0.2 in the hydrophilicity discrimination test), Riquecolor RPH-30 (trade name; Benikouji pigment water / alcohol solution; manufactured by Riken Vitamin Co., Ltd.) powdered (Distribution coefficient in the hydrophilicity discrimination test- 0.3), Riquecolor RPH-60 (trade name: Benikouji pigment water / alcohol solution; manufactured by Riken Vitamin Co., Ltd.), powdered (partition coefficient -0.4 in hydrophilicity discrimination test), Riquecolor R-4200 ( SD) (trade name; manufactured by Riken Vitamin Co., Ltd.) (distribution coefficient 0.2 in the hydrophilicity discrimination test) and the like are commercially produced and sold. These can be used for production.

Polyglycerol condensed ricinoleic acid ester The polyglycerol condensed ricinoleic acid ester used in the present invention is an esterification product of polyglycerol and condensed ricinoleic acid, and is produced by a known esterification reaction or the like. Examples of the polyglycerin include those having an average degree of polymerization of about 2 to 15. The average degree of polymerization is preferably about 3 to 10. Specifically, for example, triglycerin, tetraglycerin, hexaglycerin and the like are preferable. The condensed ricinoleic acid is a mixture obtained by heating ricinoleic acid to cause a polycondensation reaction. Examples of the condensed ricinoleic acid include those having an average degree of polymerization of about 2 to 10. The average degree of polymerization is preferably about 3 to 6.

  Moreover, in this invention, the polyglycerol condensed ricinoleic acid ester whose ratio of the emulsification phase in the emulsification ability test mentioned later is 81-89% is used as a polyglycerol condensed ricinoleic acid ester. By using such a polyglycerin condensed ricinoleic acid ester, the median diameter of the solid phase fine particles containing the beige mushroom dye in the benichow dye preparation of the present invention becomes smaller, thereby improving the light stability of the bemoji dye. Moreover, the effect of improving the light stability of food colored using the preparation can be obtained.

The emulsifying power test in the present invention is a test performed by the following method.
<Emulsifying power test>
1) In a 500 ml beaker, 200 g of rapeseed white squeezed oil (manufactured by Borso Oil & Fats Co., Ltd.) and 0.80 g of polyglycerin condensed ricinoleate are mixed and heated to 60 ° C. to obtain an oil phase.
2) The oil phase of 1) was stirred at 3000 rpm using an emulsifier (model: TK homomixer MARK II 2.5 type; manufactured by Primix Co., Ltd.) in which the gap between the mixer part and the baffle plate was fixed at 2.5 cm. Then, 200 g of purified water heated to 60 ° C. was added to the oil phase over 90 seconds.
3) Furthermore, stirring at 10000 rpm for 3 minutes while adjusting the temperature to 60 ° C., a water-in-oil emulsion is obtained.
4) Add 100 ml of the water-in-oil emulsion obtained in 3) to a 100 ml stoppered graduated cylinder and store it in a thermostat at 37 ° C. for 120 hours. After storage, the volume (ml) of the emulsified phase is measured for the contents of the graduated cylinder separated into an oil phase, an emulsified phase and an aqueous phase, and the proportion (%) of the emulsified phase is calculated based on the following formula.

  Examples of the polyglycerin condensed ricinoleic acid ester in which the proportion of the emulsified phase in the emulsifying power test is 81 to 89% include, for example, Sunsoft No. 818SK (trade name; 86% of emulsified phase in emulsifying power test; manufactured by Taiyo Kagaku Co., Ltd.), Palsgaard 4150 (trade name; 89% of the emulsified phase in emulsifying power test; manufactured by Pulseguard), SY Glister CR -ED (trade name; 81% of emulsified phase in emulsifying power test; manufactured by Sakamoto Yakuhin Kogyo Co., Ltd.) is commercially produced and sold. In the present invention, these are used as polyglycerin condensed ricinoleic acid esters. it can.

Edible fats and oils The edible fats and oils used in the present invention are not particularly limited as long as they are edible fats and oils. For example, olive oil, sesame oil, sesame oil, safflower oil, soybean oil, corn oil, rapeseed oil, palm oil, palm olein Vegetable oils such as palm kernel oil, sunflower oil, grape oil, cottonseed oil, palm oil and peanut oil are preferred. Among the edible oils and fats, a salad oil of one or more vegetable oils selected from the group consisting of sesame oil, rice oil, safflower oil, soybean oil, corn oil, rapeseed oil, sunflower oil, grape oil and cottonseed oil is more preferred, and rapeseed salad oil is particularly preferred preferable. In the present invention, one type of edible oil or fat may be used, or two or more types may be used in any combination.

Method for producing Benikouji pigment preparation Bencouge pigment formulation of the present invention comprises: (a) an aqueous phase containing Benikouji pigment; and (b) a polyglycerin condensed ricinoleic acid in which the proportion of the emulsion phase in the emulsifying power test is 81 to 89%. A step of emulsifying an ester (hereinafter, also simply referred to as polyglycerin condensed ricinoleic acid ester) and (c) an oil phase containing an edible oil and fat to obtain a water-in-oil emulsion composition, and the water-in-oil emulsion composition Can be manufactured by a manufacturing method including a step of drying under reduced pressure. Such a method for producing a beige mushroom pigment preparation is also one aspect of the present invention.

  In the present invention, first, an aqueous phase containing a beige mushroom pigment and an oil phase containing a polyglycerin-condensed ricinoleic acid ester and an edible fat are emulsified to obtain a water-in-oil emulsion composition. Specifically, polyglycerin-condensed ricinoleic acid ester is added to edible fats and oils and dissolved by heating to about 50 to 90 ° C, preferably about 60 to 80 ° C. While stirring the oil phase, slowly add an aqueous phase consisting of Benicius dye and water and / or alcohol dissolved therein at about 10-70 ° C., preferably about 20-60 ° C., for example, Claremix (model: CLM A water-in-oil emulsion composition can be obtained by a method of emulsifying at a rotational speed of about 6000 to 20000 rpm and a stirring time of about 5 to 60 minutes.

  Although there is no particular limitation on the content of Benikouji pigment in 100% by mass of the aqueous phase, for example, Benikouji pigment powder is usually about 0.1 to 50% by mass, preferably about 1 to 35% by mass, with the balance being water and It is preferable to adjust so as to be alcohol. It is preferable that the content of Benikouji pigment and water and / or alcohol in the aqueous phase is in such a range because an aqueous phase in which a sufficient amount of Benikouji pigment is dissolved can be prepared. Examples of the water used for the preparation of the aqueous phase include purified water such as distilled water, ion exchange resin treated water, reverse osmosis membrane treated water and ultrafiltration membrane treated water, and drinking water such as tap water. It is done. Moreover, as alcohol used for preparation of the said water phase, monohydric alcohols, such as ethanol and methanol, are mentioned, for example. When water and alcohol are used, water: alcohol (volume ratio) is preferably about 99: 1 to 10:90, more preferably about 95: 5 to 30:70.

  The content of polyglycerin condensed ricinoleic acid ester and edible oil / fat in 100% by mass of the oil phase is not particularly limited. For example, polyglycerin condensed ricinoleic acid ester is usually about 0.1 to 70% by mass, preferably about 0%. 0.5 to 60% by mass, more preferably about 1 to 50% by mass, and it is preferable to adjust the remainder to be edible fats and oils. It is preferable that the content of the polyglycerin condensed ricinoleic acid ester and the edible oil / fat in the oil phase is in such a range because a water-in-oil emulsion composition having good emulsion stability can be obtained.

  The aqueous phase added to the oil phase is preferably about 0.01 to 2 in terms of mass ratio with respect to the oil phase 1, for example. More preferably, about 0.05 to 1 (mass ratio) of the aqueous phase is added to the oil phase 1. Such a range is preferable because a water-in-oil emulsion composition having good emulsion stability can be obtained.

  The apparatus for producing the water-in-oil emulsion composition is not particularly limited, and for example, a normal stirring / mixing tank equipped with a stirrer, a heating jacket, a baffle plate, and the like can be used. As a stirrer to be equipped, for example, a high-speed rotation type homogenizer such as TK homomixer (manufactured by Primics) or Claremix (manufactured by M Technique) is preferably used. Moreover, you may further homogenize the liquid emulsified with these apparatuses using a high-pressure type homogenizer. Here, examples of the high-pressure homogenizer include an APV gorin homogenizer (manufactured by APV), a microfluidizer (manufactured by Microfluidics), an optimizer (manufactured by Sugino Machine), or a nanomizer (manufactured by Daiwa Steel). Can be preferably used. Instead of the homogenizer, a homogenizer such as an ultrasonic emulsifier may be used.

  Subsequently, the water-in-oil emulsion composition is dried under reduced pressure to obtain a beige mushroom pigment preparation. In vacuum drying, a known vacuum drying apparatus can be used, and there are no particular limitations on the use conditions of the vacuum drying apparatus, but the degree of vacuum in the sealed system during vacuum drying is usually about 100 to 50000 Pa, preferably about The temperature in the closed system at the time of drying under reduced pressure is usually about 30 to 90 ° C, preferably about 50 to 75 ° C. The time for drying under reduced pressure is usually about 10 to 180 minutes, preferably about 30 to 120 minutes.

  The Benikouji pigment preparation of the present invention is preferably a composition comprising a solid phase containing Bencouge pigment and an oil phase containing polyglycerin-condensed ricinoleic acid ester and edible fats and oils. More preferably, the solid phase is dispersed in the oil phase as fine particles having a median diameter of about 0.01 to 0.2 μm. In the present invention, when the median diameter of the solid phase containing a beige mushroom dye is in the above range, the stability of the bemoji dye to light is significantly improved. The median diameter of the solid phase containing the Benikouji pigment is more preferably about 0.02 to 0.17 μm, and particularly preferably about 0.03 to 0.13 μm.

  The median diameter of the solid phase in the Benikouji pigment preparation of the present invention is prepared by, for example, preparing a test solution by dispersing the Benikouji pigment formulation in hexane, and determining the median size of the solid phase fine particles in the test solution using a dynamic light scattering type particle. It can be determined by measurement using a diameter distribution measuring device (model: LB-500; manufactured by HORIBA, Ltd.) (particle diameter standard: volume, sample refractive index: 1.600, dispersion medium refractive index: 1.376). ).

  It is preferable that the water content of the beech mushroom pigment preparation of the present invention is about 5% by mass or less. In the present invention, when the water content in the preparation is in the above range, the stability of the beige mushroom pigment to light is improved, and the time-lapse fading and discoloration of the beech mushroom pigment in the case of storing the beige mushroom pigment preparation in a dark place. It is suppressed. The water content of the beige mushroom pigment preparation is more preferably about 2% by mass or less, particularly preferably about 0.5% by mass or less, and further preferably about 0.2% by mass or less.

  The water content in the beech mushroom pigment preparation obtained by the present invention can be measured based on “19. Moisture determination method (Karl Fischer method)” described in “8th Edition Food Additives Official Specification”. .

  Although there are no particular restrictions on the content of the beech powder, polyglycerin-condensed ricinoleic acid ester, and edible oil in 100% by weight of the present Bencow dye preparation, for example, the amount of the beech powder is usually about 0.05 to 35% by weight, preferably about 0.1 to 20% by mass, polyglycerin condensed ricinoleic acid ester is usually about 0.5 to 70% by mass, preferably about 1 to 50% by mass, and edible oil / fat is usually 20 to 99% by mass, preferably about 40 to 95%. It is preferable to adjust so that it may become mass%. When the content of the beech mushroom pigment, polyglycerin condensed ricinoleate and edible oil and fat is within the above-mentioned range, the stability of the beech mushroom pigment contained in the drug product to light is remarkable in the state where the beech mushroom pigment formulation is added to processed foods, etc. Therefore, it is preferable.

  In the beconic dye preparation of the present invention, for example, a dye other than the becouge dye or an antioxidant (for example, extracted tocopherol, ascorbyl palmitate, etc.) and the like are added within a range not inhibiting the purpose and effect of the present invention. May be.

  Benikouji pigment preparation of the present invention, for example, various processed foods such as bread and baked confectionery, rice cakes, delicacies, baked pork, ham sausage, salmon flakes, chili oil, chocolate, powdered seasoning, etc. It can be used for coloring processed foods and cosmetics such as lipsticks, and can be used particularly favorably for coloring of sea urchins.

  There is no restriction | limiting in particular in the method of coloring a foodstuff using the beech mushroom pigment | dye formulation of this invention, It can implement by a publicly known method. Although the amount of the beige mushroom pigment preparation added to the food varies depending on the color value, the kind of the food, and the like, it is preferably about 0.01 to 5.0% by mass, more preferably about 0.02% with respect to 100% by mass of the food. It is -3.0 mass%.

  By dispersing (a) Benikouji pigment in an oil phase containing (b) a polyglycerin condensed ricinoleic acid ester in which the proportion of the emulsified phase in the emulsifying power test is 81 to 89% and (c) edible fats and oils, It is possible to improve the stability of the beige moji pigment to light. Such a (a) bemoji dye is dispersed in an oil phase containing (b) a polyglycerin-condensed ricinoleic acid ester in which the proportion of the emulsified phase in the emulsifying power test is 81 to 89% and (c) edible fats and oils. A method for stabilizing the light of a beige mushroom dye including the steps is also one aspect of the present invention.

  The method for dispersing the beech powder dye in the oil phase is the same as the method for producing the water-in-oil emulsion composition in the above-described method for producing the bemoji dye preparation. Further, as described above, it is preferable to disperse a solid phase containing a beige powder pigment in an oil phase, and it is more preferable to disperse the solid phase as fine particles having a median diameter of about 0.01 to 0.2 μm in the oil phase. preferable. The median diameter of the solid phase containing Benikouji pigment is more preferably about 0.02 to 0.17 μm, and particularly preferably about 0.03 to 0.13 μm.

  Hereinafter, the present invention will be described more specifically based on Examples, Comparative Examples, and Test Examples, but the present invention is not limited to these.

  Table 1 shows the proportion of the emulsified phase in the following emulsification test for the polyglycerol condensed ricinoleic acid ester used in the following Examples and Comparative Examples, and the polyglycerol condensed ricinoleic acid ester.

<Emulsifying power test>
1) 200 g of rapeseed white squeezed oil (manufactured by Borso Oil & Fats) and 0.80 g of polyglycerin condensed ricinoleate were mixed in a 500 ml beaker and heated to 60 ° C. to obtain an oil phase.
2) The oil phase of 1) was stirred at 3000 rpm using an emulsifier (model: TK homomixer MARK II 2.5 type; manufactured by Primix Co., Ltd.) in which the gap between the mixer part and the baffle plate was fixed at 2.5 cm. Then, 200 g of purified water heated to 60 ° C. was added to the oil phase over 90 seconds.
3) The mixture was further stirred at 10,000 rpm for 3 minutes while adjusting the temperature to 60 ° C. to obtain a water-in-oil emulsion.
4) 100 ml of the water-in-oil emulsion of 3) was placed in a 100 ml stoppered graduated cylinder and stored in a 37 ° C. incubator for 120 hours. After storage, the volume (ml) of the emulsified phase was measured for the contents of the graduated cylinder separated into an oil phase, an emulsified phase and an aqueous phase, and the proportion (%) of the emulsified phase was calculated based on the following formula.

  Table 2 shows the distribution coefficient in the following hydrophilicity discrimination test for the powdered beech powder used in the following Examples and Comparative Examples.

＜親水性判別試験＞
１）ベニコウジ色素粉末（色価１０００換算）０．００２ｇを５０ｍｌ容共栓付比色管に入れ、イオン交換水１５ｍｌを加え、よく混合して溶解液を得た。
２）１）の溶解液に１−オクタノール（試薬特級；和光純薬工業社製）１５ｍｌを加え、前記比色管を上下に１８０度づつ回転させながら５分間振とうした（回転数２０回／分）。
３）振とう後、前記比色管の内容物を５０ｍｌ容遠心ボトルに入れ、遠心分離機を使用して遠心加速度１２０００×ｇで１５分間遠心分離を行った。
４）遠心分離後、前記遠心ボトル内の１−オクタノール相および水相を採取し、これらの相の各々について、分光光度計を使用して可視極大吸収波長における吸光度および６６０ｎｍにおける吸光度を測定した。
５）１−オクタノール相の可視極大吸収波長における吸光度をＯ１、１−オクタノール相の６６０ｎｍにおける吸光度をＯ２、水相の可視極大吸収波長における吸光度をＷ１、水相の６６０ｎｍにおける吸光度をＷ２とし、次式に基づいて分配係数を計算した。

<Hydrophilicity discrimination test>
1) 0.002 g of Benikouji dye powder (color value 1000 conversion) was put into a 50 ml capacitative colorimetric tube, and 15 ml of ion exchange water was added and mixed well to obtain a solution.
2) 15 ml of 1-octanol (special grade reagent; manufactured by Wako Pure Chemical Industries, Ltd.) was added to the solution of 1) and shaken for 5 minutes while rotating the colorimetric tube up and down 180 degrees (rotation number 20 times / Min).
3) After shaking, the contents of the colorimetric tube were placed in a 50 ml centrifuge bottle and centrifuged at 12000 × g for 15 minutes using a centrifuge.
4) After centrifugation, the 1-octanol phase and the aqueous phase in the centrifuge bottle were collected, and for each of these phases, the absorbance at the visible maximum absorption wavelength and the absorbance at 660 nm were measured using a spectrophotometer.
5) The absorbance at the visible maximum absorption wavelength of the 1-octanol phase is O1, the absorbance at 660 nm of the 1-octanol phase is O2, the absorbance at 660 nm of the aqueous phase is W1, and the absorbance at 660 nm of the aqueous phase is W2. The distribution coefficient was calculated based on the formula.

[Example 1]
1) 128 g of rapeseed salad oil (manufactured by Okamura Oil Co., Ltd.) and 16 g of polyglycerin condensed ricinoleic acid ester (commercially available product A) are placed in a 300 ml tall beaker, heated to 70 ° C., stirred and dissolved with a spatula to dissolve the oil phase did.
2) 4 g of Benikouji pigment powder a (trade name: Riquecolor R-4200 (SD); color value 2000; manufactured by Riken Vitamin Co., Ltd.) was dissolved in 12 g of ion-exchanged water and heated to 60 ° C. to obtain an aqueous phase.
3) While stirring the oil phase obtained in 1) at 4500 rpm using Claremix (model: CLM-0.8S; manufactured by M Technique Co., Ltd.), the aqueous phase obtained in 2) was added to the oil phase. Further, the mixture was stirred for 10 minutes under the conditions of 70 ° C. and 17000 rpm with the Clare mix to obtain a water-in-oil emulsion composition.
4) 50 g of the water-in-oil emulsified composition obtained in 3) was placed in a 500 ml eggplant type flask, and the temperature was 65 ° C. using a diaphragm vacuum pump (model: V-700; manufactured by Nihon Büch). The vacuum in the flask was gradually increased from 30000 Pa to 2000 Pa, and further dried under reduced pressure at 2000 Pa for 30 minutes, to obtain 46 g of Benikouji pigment preparation (Example product 1).
5) Water in the preparation was clarified by the above Karl Fischer method (device name: Karl Fischer moisture meter MKA-610; manufactured by Kyoto Electronics Industry Co., Ltd., titration reagent: Aquamicron titrant SS; manufactured by Mitsubishi Chemical Corporation, dehydrated solvent: Aquamicron dehydration It was 0.2 mass% as a result of measuring with Solvent CM;

[Example 2]
The same procedure as in Example 1 was carried out except that 16 g of polyglycerin condensed ricinoleic acid ester (commercial product B) was used instead of 16 g of polyglycerin condensed ricinoleic acid ester (commercial product A) of Example 1, Benikouji dye 46 g of the preparation (work product 2) was obtained. It was 0.2 mass% as a result of measuring the water content in this formulation similarly.

[Example 3]
The same procedure as in Example 1 was carried out except that 16 g of polyglycerin condensed ricinoleic acid ester (commercial product C) was used instead of 16 g of polyglycerin condensed ricinoleic acid ester (commercial product A) of Example 1, Benikouji dye 46 g of the preparation (Example 3) was obtained. It was 0.2 mass% as a result of measuring the water content in this formulation similarly.

[Example 4]
1) 80 g of rapeseed salad oil (manufactured by Okamura Oil Co., Ltd.) and 48 g of polyglycerin condensed ricinoleic acid ester (commercial product B) are placed in a 300 ml tall beaker, heated to 65 ° C., stirred and dissolved with a spatula to dissolve the oil phase did.
2) 4 g of Benikouji pigment powder a (trade name: Riquecolor R-4200 (SD); color value 2000; manufactured by Riken Vitamin Co., Ltd.) is dissolved in 14 g of ion-exchanged water and 14 g of ethanol (reagent grade 1, manufactured by Wako Pure Chemical Industries, Ltd.). And heated to 65 ° C. to obtain an aqueous phase.
3) While stirring the oil phase obtained in 1) at 4500 rpm using Claremix (model: CLM-0.8S; manufactured by M Technique Co., Ltd.), the aqueous phase obtained in 2) was added to the oil phase. Further, the mixture was stirred for 20 minutes at 60 ° C. and 10000 rpm with the CLEARMIX to obtain a water-in-oil emulsion composition.
4) 50 g of the water-in-oil emulsified composition obtained in 3) was placed in a 500 ml eggplant type flask, and the temperature was 65 ° C. using a diaphragm vacuum pump (model: V-700; manufactured by Nihon Büch). The vacuum in the flask was gradually increased from 30000 Pa to 2000 Pa, and further dried under reduced pressure at 2000 Pa for 30 minutes to obtain 41 g of Benikouji pigment preparation (Example product 4). It was 0.1 mass% as a result of measuring the water content in this formulation similarly.

[Example 5]
1) 200 g of Benikouji pigment water / ethanol solution (trade name: Riquecolor RPH-30; color value 63; manufactured by Riken Vitamin Co., Ltd.) is placed in a 1000 ml eggplant type flask, and a diaphragm vacuum pump (model: V-500; manufactured by Nihon Büch) ), The vacuum in the flask was gradually changed from 13000 Pa to 6000 Pa under the condition of a temperature of 45 ° C., and dried under reduced pressure until the amount of Benikouji dye solution reached 90 g, to obtain a concentrated solution of Benikouji dye water / ethanol solution. It was.
2) Put the concentrated solution of 1) into a 500 ml beaker, freeze at −80 ° C., and freeze-dry using a freeze-dryer (model: DC500; manufactured by Yamato Kagaku Co., Ltd.) at a vacuum degree of 5 Pa. 10.5 g of pigment powder b (color value 1200) was obtained.
3) 88 g of rapeseed salad oil (manufactured by Okamura Oil Co., Ltd.) and 48 g of polyglycerin condensed ricinoleic acid ester (commercial product B) are placed in a 300 ml tall beaker, heated to 70 ° C., stirred and dissolved with a spatula to dissolve the oil phase did.
4) 7 g of the beech powder pigment b of 2) was dissolved in 17 g of ion-exchanged water and heated to 60 ° C. to obtain an aqueous phase.
5) While stirring the oil phase obtained in 3) at 4500 rpm using CLEARMIX (model: CLM-0.8S; manufactured by M Technique Co., Ltd.), the aqueous phase obtained in 2) was added to the oil phase. Further, the mixture was stirred for 10 minutes under the conditions of 70 ° C. and 17000 rpm with the Clare mix to obtain a water-in-oil emulsion composition.
6) 50 g of the water-in-oil emulsion composition obtained in 5) was placed in a 500 ml eggplant type flask, and the temperature was 65 ° C. using a diaphragm vacuum pump (model: V-700; manufactured by Nihon Büch). The vacuum in the flask was gradually increased from 30000 Pa to 2000 Pa, and further dried under reduced pressure at 2000 Pa for 30 minutes to obtain 45 g of Benikouji pigment preparation (Example product 5).
7) The water content in the preparation was determined by the Karl Fischer method (equipment name: Karl Fischer moisture meter MKA-610; manufactured by Kyoto Denki Kogyo Co., Ltd., titration reagent: Aquamicron titrant SS; manufactured by Mitsubishi Chemical Corporation, dehydrated solvent: Aquamicron dehydration) It was 0.2 mass% as a result of measuring with Solvent CM;

[Example 6]
1) 200 g of Benikouji pigment water / ethanol solution (trade name: Riquecolor RPH-60; color value 63; manufactured by Riken Vitamin Co., Ltd.) is placed in a 1000 ml eggplant type flask, and a diaphragm vacuum pump (model: V-500; manufactured by Nihon Büch) ), The vacuum in the flask was gradually changed from 13000 Pa to 6000 Pa under the condition of a temperature of 45 ° C., and dried under reduced pressure until the amount of Benikouji dye solution reached 90 g, to obtain a concentrated solution of Benikouji dye water / ethanol solution. It was.
2) Put the concentrated solution of 1) into a 500 ml beaker, freeze at −80 ° C., and freeze-dry using a freeze-dryer (model: DC500; manufactured by Yamato Kagaku Co., Ltd.) at a vacuum degree of 5 Pa. 10.9 g of pigment powder c (color value 1150) was obtained.
3) 88 g of rapeseed salad oil (manufactured by Okamura Oil Co., Ltd.) and 48 g of polyglycerin condensed ricinoleic acid ester (commercial product B) are placed in a 300 ml tall beaker, heated to 70 ° C., stirred and dissolved with a spatula to dissolve the oil phase did.
4) 7 g of the beech powder pigment c of 2) was dissolved in 17 g of ion-exchanged water and heated to 60 ° C. to obtain an aqueous phase.
5) While stirring the oil phase obtained in 3) at 4500 rpm using CLEARMIX (model: CLM-0.8S; manufactured by M Technique Co., Ltd.), the aqueous phase obtained in 4) was added to the oil phase. Further, the mixture was stirred for 10 minutes under the conditions of 70 ° C. and 17000 rpm with the Clare mix to obtain a water-in-oil emulsion composition.
6) 50 g of the water-in-oil emulsion composition obtained in 5) was placed in a 500 ml eggplant type flask, and the temperature was 65 ° C. using a diaphragm vacuum pump (model: V-700; manufactured by Nihon Büch). The vacuum in the flask was gradually increased from 30000 Pa to 2000 Pa, and further dried under reduced pressure at 2000 Pa for 30 minutes to obtain 45 g of Benikouji pigment preparation (Example 6).
7) The water content in the preparation was determined by the Karl Fischer method (equipment name: Karl Fischer moisture meter MKA-610; manufactured by Kyoto Denki Kogyo Co., Ltd., titration reagent: Aquamicron titrant SS; manufactured by Mitsubishi Chemical Corporation, dehydrated solvent: Aquamicron dehydration) It was 0.2 mass% as a result of measuring with Solvent CM;

[Example 7]
1) 400 g of Benikouji pigment water / ethanol solution (trade name: Riquecolor RPH-30; color value 63; manufactured by Riken Vitamin Co., Ltd.) is placed in a 2000 ml eggplant type flask, and a diaphragm vacuum pump (model: V-500; manufactured by Nihon Büch) ), The vacuum degree in the flask is gradually changed from 13000 Pa to 6000 Pa under the condition of a temperature of 45 ° C., and dried under reduced pressure until the amount of Benikouji dye solution reaches 180 g, thereby obtaining a concentrated solution of Benikouji dye water / ethanol solution. It was.
2) Put the concentrated solution of 1) into a 500 ml beaker, freeze at −80 ° C., and freeze-dry using a freeze-dryer (model: DC500; manufactured by Yamato Kagaku Co., Ltd.) at a vacuum degree of 5 Pa. 21.0 g of dye powder (color value 1200) was obtained.
3) Place 13.0 g of the beige powder pigment powder of 2) into a 500 ml beaker, add 42 g of ion exchange water and 45 g of ethanol (reagent grade 1, Wako Pure Chemical Industries, Ltd.) and dissolve it in acetone (reagent grade, 200 g (manufactured by Wako Pure Chemical Industries, Ltd.) was gradually added (10 g / min), and the mixture was further stirred for 15 minutes.
4) After stirring, the precipitate fraction in the beaker was collected by decantation, put into a 500 ml eggplant type flask, and vacuumed in the flask using a diaphragm vacuum pump (model: V-500; manufactured by Nihon Büch). The pressure was reduced to 500 Pa and dried under reduced pressure to obtain 8.2 g of Benikouji pigment powder d (color value 960).
5) 88 g of rapeseed salad oil (manufactured by Okamura Oil Co., Ltd.) and 48 g of polyglycerin condensed ricinoleic acid ester (commercial product B) are placed in a 300 ml tall beaker, heated to 70 ° C., stirred and dissolved with a spatula to dissolve the oil phase did.
6) The beech powder pigment d7g of 4) was dissolved in 17 g of ion-exchanged water and heated to 60 ° C. to obtain an aqueous phase.
7) While stirring the oil phase obtained in 5) at 4500 rpm using CLEARMIX (model: CLM-0.8S; manufactured by M Technique Co., Ltd.), the aqueous phase obtained in 2) was added to the oil phase. Further, the mixture was stirred for 10 minutes under the conditions of 70 ° C. and 17000 rpm with the Clare mix to obtain a water-in-oil emulsion composition.
8) 50 g of the water-in-oil emulsified composition obtained in 7) is put into a 500 ml eggplant type flask, and the temperature is 65 ° C. using a diaphragm vacuum pump (model: V-700; manufactured by Nihon Büch). The vacuum inside the flask was gradually increased from 30000 Pa to 2000 Pa, and further dried under reduced pressure at 2000 Pa for 30 minutes to obtain 45 g of Benikouji pigment preparation (Example product 7).
9) The water in the preparation was clarified by the Karl Fischer method (equipment name: Karl Fischer moisture meter MKA-610; manufactured by Kyoto Electronics Industry Co., Ltd., titration reagent: Aquamicron titrant SS; manufactured by Mitsubishi Chemical Corporation, dehydrated solvent: Aquamicron dehydration) It was 0.2 mass% as a result of measuring with Solvent CM;

[Comparative Example 1]
The same procedure as in Example 1 was carried out except that 16 g of polyglycerin condensed ricinoleic acid ester (commercial product D) was used instead of 16 g of polyglycerin condensed ricinoleic acid ester (commercial product A) of Example 1, Benikouji dye 46 g of the preparation (Comparative product 1) was obtained. It was 0.2 mass% as a result of measuring the water | moisture content in this formulation similarly.

[Comparative Example 2]
The same procedure as in Example 1 was carried out except that 16 g of polyglycerin condensed ricinoleic acid ester (commercial product E) was used instead of 16 g of polyglycerin condensed ricinoleic acid ester (commercial product A) of Example 1, Benikouji dye 46 g of the preparation (Comparative product 2) was obtained. It was 0.2 mass% as a result of measuring the water | moisture content in this formulation similarly.

[Test Example 1] Particle size measurement of Benikouji pigment in the formulation Examples 1-7 and Benikouji pigment formulations produced in Comparative Examples 1 and 2 (Examples 1-7 and Comparative products 1 and 2), respectively, in the formulation The median diameter of the solid phase microparticles containing the benichouji dye was measured. Specifically, a test liquid is prepared by dispersing Benikouji pigment preparations (Examples 1 to 7 and Comparative products 1 and 2) in hexane, and the median diameter of solid phase fine particles in the test liquid is dynamically scattered. It was measured using a formula particle size distribution measuring apparatus (model: LB-500; manufactured by HORIBA, Ltd.) (particle diameter standard: volume, sample refractive index: 1.600, dispersion medium refractive index: 1.376).
The results are shown in Table 3.

  From the results of Table 1 and Table 3, when using a polyglycerin condensed ricinoleic acid ester in which the proportion of the emulsified phase in the emulsifying power test is within the range of 81 to 89%, a case outside this range is used. Compared to the above, it was clear that a beige mushroom dye preparation having a remarkably small median diameter of the solid phase fine particles containing the bemoji dye can be obtained.

[Test Example 2] Light stability test of colored sea urchin (1)
The frozen cooked surimi (manufactured by Daihyo Co., Ltd.) was thawed and crushed until uniform using a food processor (model: MK-K48; manufactured by Matsushita Electric Industrial Co., Ltd.). To 120 g of the crushed cooked surimi, 2.6 g of Benikouji pigment preparations produced in Examples 1 to 3 and Comparative Examples 1 and 2 (Examples 1 to 3 and Comparative Products 1 and 2) or Benikouji pigment produced in Example 4 2.3 g of the preparation (practical product 4) was added and mixed with a spatula until uniform to obtain a colored surimi. 90 g of the obtained colored surimi was placed in a heat-resistant bag made of nylon (15 × 24 cm) and vacuum-packed, and the colored surimi in the bag was uniformly arranged to a thickness of about 10 mm. The colored surimi packed in a vacuum package was heated in a warm water bath at 88 ° C. for 30 minutes to prepare a colored sea urchin sample. In addition, as a control, the same was used with 2.3 g of an aqueous solution of Benikomiji dye / ethanol (control product A: trade name: Riquecolor R-30; color value 63; manufactured by Riken Vitamin Co., Ltd.), which has been conventionally used for coloring of sea urchins. A colored surimi was prepared.

  Half of the vacuum-packed colored sea urchin sample is covered with aluminum foil from the outside of the package and shielded from light. Under the conditions of 10 ° C. and 6700 lux, the light stability tester (model: PT-400NC; manufactured by Nippon Medical Chemical Co., Ltd.) ) For 6 hours. After storage, the colored sea urchin sample is fixed from the outside of the package using a black sample holder, and the color tone (L of the light-irradiated part and light-shielded part) is measured with a spectroscopic colorimeter (model: SE-2000, manufactured by Nippon Denshoku Industries Co., Ltd.). * A * b * value) (measured diameter: diameter 10 mm), and based on the measured color tone and the following formula, L * a * b * color difference (ΔE * (ab)) ) Was calculated. The results are shown in Table 4.

  In the above formula, L * represents lightness. a * represents the chromaticity in the red direction. b * represents chromaticity in the yellow direction. ΔE * (ab) represents the degree of discoloration.

  From the results shown in Tables 1 and 4, according to Benicowian dye preparations (practical products 1 to 5) produced using polyglycerin condensed ricinoleic acid ester in which the proportion of the emulsified phase in the emulsifying power test is in the range of 81 to 89%. The colored sea urchin has a smaller color difference due to light irradiation compared to the sea urchin colored by a beige mushroom pigment preparation (Comparative products 1 and 2 and Control product A) produced using a polyglycerin condensed ricinoleic acid ester outside the above range. In particular, the stability to light was improved. In addition, the kamaboko colored by the beige mushroom pigment preparation produced by using the polyglycerin condensed ricinoleic acid ester in the range of 81 to 89% of the emulsified phase in the emulsifying power test has been conventionally used for coloring kamaboko. In comparison with the eelworm pigmented water / ethanol solution, the stability to light was relatively improved.

[Test Example 3] Light stability test of colored sea urchin (2)
The frozen cooked surimi (manufactured by Daihyo Co., Ltd.) was thawed and crushed until uniform using a food processor (model: MK-K48; manufactured by Matsushita Electric Industrial Co., Ltd.). To 120 g of the crushed cooked surimi, 1.0 g of Benikouji pigment preparation (Example 4) produced in Example 4 and 1.0 g of Benikouji pigment preparation (Example 5) produced in Example 5 were produced in Example 6. 1.1 g of Benikouji pigment preparation (Example 6) or 1.3 g of Benikouji pigment preparation (Example 7) produced in Example 7 were added and mixed with a spatula until uniform to obtain a colored surimi. 90 g of the obtained colored surimi was placed in a heat-resistant bag made of nylon (15 × 24 cm) and vacuum-packed, and the colored surimi in the bag was uniformly arranged to a thickness of about 10 mm. The colored surimi packed in a vacuum package was heated in a warm water bath at 88 ° C. for 30 minutes to prepare a colored sea urchin sample. As a control, 1.0 g of Benikouji pigment water / ethanol solution (control product A: trade name: Riquet color R-30; color value 63; manufactured by Riken Vitamin Co., Ltd.) or Benikouji pigment water, which has been conventionally used for coloring of sea urchins. A colored surimi was similarly prepared using 1.0 g of an ethanol solution (control product B; trade name: Riquecolor RPH-30; color value 63; manufactured by Riken Vitamin Co., Ltd.).

  Half of the vacuum-packed colored sea urchin sample is covered with aluminum foil from the outside of the package, shielded from light, and subjected to a light stability tester (model: PT-400NC; manufactured by Nippon Medical Instruments Co., Ltd.) at 10 ° C and 3300 lux. ) For 5 hours. After storage, the colored sea urchin sample is fixed from the outside of the package using a black sample holder, and the color tone (L of the light-irradiated part and light-shielded part) is measured with a spectroscopic colorimeter (model: SE-2000, manufactured by Nippon Denshoku Industries Co., Ltd.). * A * b * value) (measured diameter: diameter 10 mm), and based on the measured color tone and the following formula, L * a * b * color difference (ΔE * (ab)) ) Was calculated. The results are shown in Table 5.

  In the above formula, L * represents lightness. a * represents the chromaticity in the red direction. b * represents chromaticity in the yellow direction. ΔE * (ab) represents the degree of discoloration.

  From the results of Table 1, Table 2 and Table 5, Benikouji pigment preparations manufactured using polyglycerin condensed ricinoleic acid esters in which the proportion of the emulsified phase in the emulsifying power test is in the range of 81 to 89% (practical products 4 to 4). 7), a Benikouji pigment preparation produced by using Benikouji pigment powder in which the distribution coefficient of Benikouji pigment powder is in the range of -1.2 to -0.1 in the hydrophilicity discrimination test (Examples 5 to 7) ), The color difference due to light irradiation is smaller than that of the sea bream colored by the bene moji pigment preparation (practical product 4) produced using the beni moji pigment powder whose distribution coefficient is outside the range. The stability against light was further improved.

Claims (3)

(A) Benikouji pigment preparation, (b) Benikouji pigment preparation containing polyglycerin condensed ricinoleic acid ester having an emulsified phase ratio of 81 to 89% in the following emulsifying power test and (c) edible oil and fat.
<Emulsifying power test>
1) In a 500 ml beaker, 200 g of rapeseed white squeezed oil (manufactured by Borso Oil & Fats Co., Ltd.) and 0.80 g of polyglycerin condensed ricinoleate are mixed and heated to 60 ° C. to obtain an oil phase.
2) The oil phase of 1) was stirred at 3000 rpm using an emulsifier (model: TK homomixer MARK II 2.5 type; manufactured by Primix Co., Ltd.) in which the gap between the mixer part and the baffle plate was fixed at 2.5 cm. Then, 200 g of purified water heated to 60 ° C. was added to the oil phase over 90 seconds.
3) Furthermore, stirring at 10000 rpm for 3 minutes while adjusting the temperature to 60 ° C., a water-in-oil emulsion is obtained.
4) Add 100 ml of the water-in-oil emulsion obtained in 3) to a 100 ml stoppered graduated cylinder and store it in a thermostat at 37 ° C. for 120 hours. After storage, the volume (ml) of the emulsified phase is measured for the contents of the graduated cylinder separated into an oil phase, an emulsified phase and an aqueous phase, and the proportion (%) of the emulsified phase is calculated based on the following formula.

The beige mushroom dye preparation according to claim 1, wherein the beige mushroom dye is a bemoji dye powder, and the partition coefficient when the following hydrophilicity discrimination test is performed on the bemoji dye powder is −1.2 to −0.1.
<Hydrophilicity discrimination test>
1) Put 0.002 g of Benikouji pigment powder (converted to a color value of 1000) into a 50 ml capacitative colorimetric tube, add 15 ml of ion exchange water, and mix well to obtain a solution.
2) Add 15 ml of 1-octanol (special grade reagent; manufactured by Wako Pure Chemical Industries, Ltd.) to the solution of 1) and shake for 5 minutes while rotating the colorimetric tube 180 degrees up and down (rotation number 20 times / Min).
3) After shaking, the contents of the colorimetric tube are placed in a 50 ml centrifuge bottle and centrifuged for 15 minutes at a centrifugal acceleration of 12000 × g using a centrifuge.
4) After centrifugation, the 1-octanol phase and the aqueous phase in the centrifuge bottle are collected, and the absorbance at the visible maximum absorption wavelength and the absorbance at 660 nm are measured for each of these phases using a spectrophotometer.
5) The absorbance at the visible maximum absorption wavelength of the 1-octanol phase is O1, the absorbance at 660 nm of the 1-octanol phase is O2, the absorbance at 660 nm of the aqueous phase is W1, and the absorbance at 660 nm of the aqueous phase is W2. Calculate the distribution coefficient based on the formula.

(A) an aqueous phase containing Benikouji pigment, and (b) a polyglycerin condensed ricinoleic acid ester in which the proportion of the emulsified phase in the following emulsifying power test is 81 to 89% and (c) an oil phase containing edible fats and oils. A method for producing a beige mushroom pigment preparation, comprising a step of emulsifying to obtain a water-in-oil emulsion composition and a step of drying the water-in-oil emulsion composition under reduced pressure.
<Emulsifying power test>
1) In a 500 ml beaker, 200 g of rapeseed white squeezed oil (manufactured by Borso Oil & Fats Co., Ltd.) and 0.80 g of polyglycerin condensed ricinoleate are mixed and heated to 60 ° C. to obtain an oil phase.
2) The oil phase of 1) was stirred at 3000 rpm using an emulsifier (model: TK homomixer MARK II 2.5 type; manufactured by Primix Co., Ltd.) in which the gap between the mixer part and the baffle plate was fixed at 2.5 cm. Then, 200 g of purified water heated to 60 ° C. was added to the oil phase over 90 seconds.
3) Furthermore, stirring at 10000 rpm for 3 minutes while adjusting the temperature to 60 ° C., a water-in-oil emulsion is obtained.
4) Add 100 ml of the water-in-oil emulsion obtained in 3) to a 100 ml stoppered graduated cylinder and store it in a thermostat at 37 ° C. for 120 hours. After storage, the volume (ml) of the emulsified phase is measured for the contents of the graduated cylinder separated into an oil phase, an emulsified phase and an aqueous phase, and the proportion (%) of the emulsified phase is calculated based on the following formula.