JP5299946B2 - 粒子の選別法 - Google Patents
粒子の選別法 Download PDFInfo
- Publication number
- JP5299946B2 JP5299946B2 JP2008047819A JP2008047819A JP5299946B2 JP 5299946 B2 JP5299946 B2 JP 5299946B2 JP 2008047819 A JP2008047819 A JP 2008047819A JP 2008047819 A JP2008047819 A JP 2008047819A JP 5299946 B2 JP5299946 B2 JP 5299946B2
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- Prior art keywords
- charged particles
- particles
- particle
- sorting
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- Prior art date
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- 239000002245 particle Substances 0.000 title claims description 32
- 238000000034 method Methods 0.000 title claims description 7
- 230000005684 electric field Effects 0.000 claims description 9
- 230000003993 interaction Effects 0.000 claims description 3
- 210000000349 chromosome Anatomy 0.000 description 19
- 210000004027 cell Anatomy 0.000 description 4
- 239000007850 fluorescent dye Substances 0.000 description 4
- 238000010186 staining Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000975 dye Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0652—Sorting or classification of particles or molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1028—Sorting particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1029—Particle size
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Fluid Mechanics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrostatic Separation (AREA)
- Separation Of Solids By Using Liquids Or Pneumatic Power (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Description
1.全体装置は、数センチ角のマイクロ流体デバイスチップと任意の電圧波形を発生するノートタイプコンピューターから成るので、省スペース(コンパクト)であり、従来のソーターの1/100以下の省電力で動作可能である。
2.基本的には蛍光色素による染色を必要としないので、力学的および化学的な損傷はほとんどない。
3.数ボルト程度の電圧印加により、選別が可能であり、また、両極性の電圧を印加するので、電気泳動のようなジュール熱は発生しない。
4.マイクロ流体デバイスチップの表面処理を行い、生体分子あるいはコロイド粒子とチップ表面との相互作用力、たとえば、静電引力、化学・物理吸着力、摩擦力を制御することにより、より精密かつ短時間で選別可能となる。
5.細胞や血球は、その生死により電気的な応答特性や表面の状態が変化するので、4との組み合わせにより生死による選別や判別が可能である。
Claims (2)
- 1mm以下のサイズの異なる荷電粒子をマイクロ流体デバイスチップに収容し、粒子を選別する方法であって、生体分子あるいはコロイド粒子と該チップ表面との相互作用力を制御することにより、前記粒子を選別し、
両極性の電圧変調波形を有する電圧を印加して、前記荷電粒子に対し交流電場を与え、
大きなサイズの荷電粒子が小さなサイズの荷電粒子に比較して、より低い周波数の交流電場において応答できなくなる振動挙動特性を利用して、小さいサイズの荷電粒子の移動度が大きいサイズの荷電粒子の移動度より大きいことにより、小さいサイズの荷電粒子をより遠くに移動させて、粒子サイズの選別を行うことを特徴とする粒子選別法。 - 1mm以下のサイズの異なる荷電粒子を選別するためのマイクロ電極を備えたマイクロ流体デバイスチップであって、生体分子あるいはコロイド粒子と該チップ表面との相互作用力を制御して、前記粒子を選別し、
前記電極に印加する両極性の電圧変調波形を発生する電圧波形発生装置とを備え、
前記電極に前記電圧変調波形の電圧を印加して前記荷電粒子に対し交流電場を与え、大きなサイズの荷電粒子が小さなサイズの荷電粒子に比較して、より低い周波数の交流電場において応答できなくなる振動挙動特性を利用して、小さいサイズの荷電粒子の移動度が大きいサイズの荷電粒子の移動度より大きいことにより、小さいサイズの荷電粒子をより遠くに移動させて、粒子サイズの選別を行うことを特徴とする粒子選別装置。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008047819A JP5299946B2 (ja) | 2008-02-28 | 2008-02-28 | 粒子の選別法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008047819A JP5299946B2 (ja) | 2008-02-28 | 2008-02-28 | 粒子の選別法 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2003036532A Division JP2004243238A (ja) | 2003-02-14 | 2003-02-14 | 粒子の選別法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2008188592A JP2008188592A (ja) | 2008-08-21 |
JP5299946B2 true JP5299946B2 (ja) | 2013-09-25 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2008047819A Expired - Fee Related JP5299946B2 (ja) | 2008-02-28 | 2008-02-28 | 粒子の選別法 |
Country Status (1)
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JP (1) | JP5299946B2 (ja) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4586903B2 (ja) | 2008-07-22 | 2010-11-24 | 株式会社デンソー | エンジン制御装置 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002233791A (ja) * | 2001-02-08 | 2002-08-20 | Mitsubishi Research Institute Inc | 微粒子の分級および配列方法 |
JP2004243238A (ja) * | 2003-02-14 | 2004-09-02 | National Institute Of Advanced Industrial & Technology | 粒子の選別法 |
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2008
- 2008-02-28 JP JP2008047819A patent/JP5299946B2/ja not_active Expired - Fee Related
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JP2008188592A (ja) | 2008-08-21 |
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