JP5206270B2 - Method for cutting laminate and method for producing strip using the same - Google Patents

Description

  The present invention relates to a method for cutting a laminate and a method for producing a strip using the method.

As a detection device for detecting a substance to be detected in a liquid sample by chromatography, there is a strip-shaped detection device called a chromatography strip. Usually, this strip is manufactured by a method of cutting a laminate comprising an adhesive sheet and a plurality of members arranged on the adhesive sheet so as to contact the adhesive surface. As a means for cutting the laminate, a method using a blade is generally used, but a method of trimming with a laser is also being studied (see Patent Document 1).
Japanese Patent No. 3542999

  However, when the strip is cut out by cutting the laminate, many defective products such as a member peeled off from the adhesive sheet may occur. In general, in the above laminate for obtaining a strip, a plurality of members partially overlap each other on the adhesive sheet, and thus the thickness of the laminate is non-uniform. It is thought that defective products are likely to occur. In particular, in the case of a chromatographic strip for collecting a liquid sample directly from a living body, it is required to reduce the width of the strip so that it can be detected even with a small amount of liquid sample. There is a tendency for defective products to become apparent.

  Although the generation of defective products can be suppressed to some extent by the method using the laser described in Patent Document 1, a simpler method is required because it requires special machines and operations.

  Therefore, the present invention provides a simple method that makes it possible to suppress the occurrence of defective products due to peeling of members or the like even when a laminated body having a non-uniform thickness is cut into a thin strip shape. For the purpose.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors remarkably suppress the occurrence of defective products by a simple method of stacking a mount on the laminate and cutting the laminate together with the mount. The present invention has been completed.

  That is, the present invention includes a sheet-like support member and a plurality of members provided in contact with one surface of the support member, and two or more of the plurality of members overlap each other and a portion that does not overlap. A method of cutting a laminated body so that an overlapping portion and a non-overlapping portion are cut together with a supporting member, wherein a mount is disposed on the other surface side of the supporting member and / or the plurality of member sides, and the laminated body Is a method of cutting the laminate together with the mount from the side of the plurality of members in a state where is stacked on the mount.

  In a conventional cutting method that does not use a mount, when the strength of the laminate is weak, deformation may occur such as bending of the laminate in the direction in which the force is applied when stress is applied during cutting. If the laminate is cut in such a deformed state, the member is likely to be peeled off. However, in the method according to the present invention, by placing the mount on the other surface side of the support member and / or the plurality of members, that is, the mount is placed on the lower side and / or the upper side of the laminate, By cutting in a state of being stacked on the mount, the laminate is supported by the mount and has a strength capable of resisting stress applied during cutting, and the laminate is less likely to be deformed when cut. As a result, even when a laminated body having a non-uniform thickness is cut into strips having a narrow width, it is possible to suppress the occurrence of defective products due to member peeling or the like. Further, according to the method of the present invention, a special machine or operation is not required, and a strip can be easily obtained.

  The plurality of members are a plurality of strip-shaped members arranged in parallel to each other, and the laminate can be cut so that a strip having a predetermined width is cut out along a direction orthogonal to the longitudinal direction thereof. preferable. According to such a method, it is possible to efficiently manufacture a large-width strip having a similar structure in a large amount.

  The predetermined width is preferably 0.5 mm to 3 mm, more preferably 0.8 mm to 2.5 mm, and still more preferably 1 mm to 2 mm. According to the method of the present invention, the occurrence of defective products is sufficiently suppressed even when the width is narrow. In addition, when the width is less than 3 mm, a strip suitable for use as a chromatographic strip for collecting a liquid sample directly from a living body can be obtained. On the other hand, when the width is less than 0.5 mm, the effect of suppressing defective products tends to be reduced.

  As the mount, a sheet-like paper material is preferably used. The mount disposed on the other surface side of the support member and the mount disposed on the plurality of member sides of the support member may be the same or different. For example, both thicknesses may be the same or different. The thickness of the mount is preferably 0.03 mm to 0.6 mm. The thickness of the mount on the other side of the support member is more preferably 0.05 mm to 0.5 mm, and still more preferably 0.08 mm to 0.4 mm. The thickness of the mount on the plurality of member sides of the support member is more preferably 0.03 mm to 0.1 mm, and still more preferably 0.04 mm to 0.07 mm. When the mount has a thickness in such a range, the laminated body is more reliably prevented from being damaged, and the effect of suppressing the generation of defective products at the time of cutting is particularly noticeable.

  The support member is preferably liquid-impermeable, and the plurality of members include a member having a non-woven fabric, a member having a porous membrane, and a member formed of cellulose, and these members are arranged in this order. It is preferable that it is arranged above. By cutting such a laminate, a strip particularly suitable for use as a chromatographic strip can be obtained. In the chromatographic strip, a liquid sample sequentially moves through a member having a nonwoven fabric, a member having a porous membrane, and a member formed of cellulose by capillary action. At this time, since the support member is liquid-impermeable, it is possible to prevent the liquid sample from leaking from the back surface of these members.

  The laminate preferably includes an adhesive member that is bonded to the surface of the two members that overlap each other on the surface opposite to the support member so that the end of one member is fixed to the other member. . When the laminate includes such an adhesive member, the members can be prevented from peeling off. The adhesive member is not limited as long as the overlapping members are fixed to such an extent that the overlapping members do not peel off, and examples thereof include a paper adhesive tape. The length of the adhesive member is preferably substantially the same as the length in the direction perpendicular to the cutting direction of the laminate.

  In the method according to the present invention, the laminate may be cut with a single blade whose only one side of the blade edge is inclined. In general, when a laminated body is cut with a single blade, the side cut by the back surface (flat surface) of the blade edge produces a clean cut surface, and the cut surface of the side cut by the inclined blade surface is crushed. Transforms into When the member is pushed downward by the inclined blade surface and tilted downward, the member on the back surface side of the blade edge is lifted, and the members may be separated from each other. Cutting with a single blade may easily cause peeling of the member. However, according to the method of the present invention, it is possible to suppress peeling of the member even when cutting with a single blade.

  In the method according to the present invention, a laminate using a guillotine-type cutting machine, which includes a movable blade and a fixed blade arranged so that the blade edges face each other, and the movable blade is a single blade whose only one surface of the blade edge is inclined. May be cut. According to such a guillotine-type cutting machine, a strip having a constant width can be manufactured in a large amount efficiently and accurately.

  On the other hand, when the laminated body is cut using such a guillotine-type cutting machine, the member is peeled off by contacting the cut surface of the laminated body when the movable blade moves away from the fixed blade after cutting. It is conceivable that this is likely to occur. However, according to the method of the present invention, even when cutting using a guillotine-type cutting machine, it is possible to sufficiently suppress the occurrence of defective products due to peeling of members or the like.

  In another aspect, the present invention relates to a method for producing a strip, comprising the step of cutting a laminate by the above method to cut out a strip. According to this manufacturing method, even when a laminated body having a non-uniform thickness is cut into a thin strip shape, it is possible to easily manufacture while suppressing the generation of defective products due to peeling of members or the like. .

  The production method according to the present invention is particularly useful, for example, as a method for producing a chromatography strip for detecting a substance to be detected in a liquid sample by chromatography.

  According to the present invention, there is provided a simple method that makes it possible to suppress generation of defective products due to peeling of members or the like even when a laminated body having a non-uniform thickness is cut into a thin strip shape. The

  Hereinafter, the best mode for carrying out the present invention will be described in detail with reference to the drawings as necessary. However, the present invention is not limited to the following embodiments. In the drawings, the same elements are denoted by the same reference numerals, and redundant description is omitted. Further, the dimensional ratios in the drawings are not limited to the illustrated ratios.

  FIG. 1 is a side end view of one embodiment of a strip. A strip 1 shown in FIG. 1 is a chromatographic strip for detecting IgE antibody (a substance to be detected) in human tears (liquid sample) by immunochromatography. The strip 1 has, for example, a strip shape (elongated shape such as a strip or a strip) having a width of about 1.5 mm and a length of about 57 mm, and a mass of about 0.03 g. The strip 1 includes a sheet-like support member 18, a sampling member 10 provided on the support member 18 in contact with one surface of the support member 18, a holding member 12, a detection member 14, an absorption member 16, a sampling member 10, A first adhesive member 20a bonded to the surface of the holding member 12 and the detection member 14 opposite to the support member 18 so that the end of the holding member 12 is fixed to the detection member 14; The member 14 and the absorbing member 16 are bonded to the surface of the absorbing member 16 opposite to the supporting member 18 so that the end of the absorbing member 16 is fixed to the detecting member 14, and the absorbing member 16 and the sampling member of the supporting member 18 are collected. A second adhesive member 20b extending so as to sandwich an end opposite to the 10 side, and a background member 22 provided in contact with the other surface of the support member 18 are provided.

  The collection member 10, the holding member 12, the detection member 14, and the absorption member 16 are arranged in the longitudinal direction of the strip 1 on the support member 18 so that the tear fluid moves in these order by capillary action in these members. It is arranged. The holding member 12 and the detection member 14 partially overlap each other when a part of the holding member 12 rides on the surface of the detection member 14 opposite to the support member 18. In other words, the holding member 12 and the detection member 14 have portions that overlap each other and portions that do not overlap when viewed from a direction perpendicular to the main surface of the support member 18. Similarly, the detection member 14 and the absorption member 16 partially overlap each other when a part of the absorption member 16 rides on the surface of the detection member 14 opposite to the support member 18. Due to such overlapping of the members, the thickness of the strip 1 is not uniform.

  The sampling member 10 is a member made of a fiber base material such as a pulp nonwoven fabric or polyester. The collecting member 10 includes a protruding portion 10a protruding from the support member 18 on the upstream side (hereinafter simply referred to as “upstream side”) in the lacrimal fluid movement direction, and a non-projecting portion 10b that is the other portion. Including. The protruding portion 10a is exposed without being covered by other members such as the support member 18 and the first adhesive member 20a. Moreover, the protrusion part 10a is strip shape, and has a flat surface in an edge part. The length of the protruding portion 10a is preferably 5 mm or more.

  When collecting human tears using the strip 1, the protruding portion 10 a is inserted into the lower conjunctival dome of the subject, and tears are collected with the strip 1 suspended in the vertical direction. At this time, since the protruding portion 10a is made of a fiber base material such as a pulp nonwoven fabric or polyester, it becomes easy to absorb tears. Moreover, since such a fiber base material is a weak irritation | stimulation material, even if the protrusion part 10a touches an eyeball, it is hard to give pains, such as a pain, to a subject. Furthermore, since the protruding portion 10a has a strip shape, a series of operations are facilitated. In particular, the protruding portion 10a has a flat surface, and the surface of the protruding portion 10a comes into contact with the sample collection site, thereby causing pain to the subject. Can be further reduced. Moreover, when the length of the protrusion part 10a is 5 mm or more, it can prevent that members other than the collection member 10 touch an eyeball etc. of a subject.

  The tear fluid absorbed by the collecting member 10 moves to the holding member 12 by capillary action. The holding member 12 includes a fiber base material such as a pulp nonwoven fabric or polyester, and a labeling reagent that specifically binds to the IgE antibody. This labeling reagent is obtained by labeling an antibody having an IgE antibody as an antigen with a gold colloid (labeling substance). The labeling reagent is held on the fiber substrate, and when it comes into contact with tears, it elutes into the tears and can move with the movement of the tears. In the process in which the labeling reagent moves in the holding member 12 and the detection member 14 along with the movement of the tear, the labeling reagent binds to the IgE antibody in the tear and forms a conjugate of the IgE antibody and the labeling reagent.

  As the labeling substance, latex beads and the like can be used besides gold colloid. However, it is preferable to use red or blue colored particles that do not require a special device or the like for confirmation of the label and can be easily confirmed visually.

  The holding member 12 shares a single fiber base material with the sampling member 10, and the labeling reagent is held at the downstream end (hereinafter simply referred to as “downstream side”) of the fiber base material in the tear fluid movement direction. As a result, the holding member 12 is formed. Thus, the strength of the strip 1 can be increased by integrating the sampling member 10 and the holding member 12. In addition, by using a single fiber base material, the liquid sample can easily move from the collection member 10 to the holding member 12 by capillary action. When the sampling member 10 and the holding member 12 are not integrated, it is preferable that the two members partially overlap in order to maintain the strength of the strip 1. Prone. In that respect, in the strip 1 shown in FIG. 1, the total volume of both members can be reduced by integration. If the total volume of both members is small, the volume of tears permeates becomes small, so that a sufficient detection result can be obtained with a smaller amount of collection.

  The holding member 12 partially overlaps the detection member 14 by partially riding on the detection member 14. Thereby, since the contact area of the holding member 12 and the detection member 14 becomes large, the intensity | strength of the strip 1 increases. In addition, the tear fluid can easily move from the holding member 12 to the detection member 14 by capillary action.

  The length of the portion where the holding member 12 and the detection member 14 overlap is preferably 1 to 1.5 mm. If the length of the overlapping portion is less than 1 mm, the members tend to be peeled apart, and if the length is longer than 1.5 mm, the volume of the portion into which tear fluid permeates increases. It tends to be difficult to collect tear fluid.

  The detection member 14 includes a nitrocellulose film 14c, which is a porous membrane, and a detection reagent and a control reagent fixed to the nitrocellulose film 14c. The detection reagent is fixed to the detection reagent fixing part 14a on the nitrocellulose membrane 14c. The detection reagent fixing portion 14 a extends linearly along a direction orthogonal to the longitudinal direction of the strip 1. The detection reagent is an antibody having the same or different IgE antibody as an antigen as the antibody of the labeling reagent. The detection reagent specifically binds to the IgE antibody, thereby capturing the conjugate of the IgE antibody and the labeling reagent. As described above, when the detection reagent captures the conjugate, a red line appears in the detection reagent fixing portion 14a. By visually confirming this line, it is determined that the IgE antibody is present in the tear fluid. be able to.

  The control reagent is fixed to the control reagent fixing part 14b located on the downstream side of the detection reagent fixing part 14a on the nitrocellulose membrane 14c. The control reagent fixing part 14b extends linearly along a direction orthogonal to the longitudinal direction of the strip 1. The control reagent is an antibody having the antibody contained in the labeling reagent as an antigen. When the control reagent captures the labeled reagent in the transferred tears, a red line appears in the control reagent fixing part 14b. By visually checking this line, the tears move to the control reagent fixing part 14b. That is, it can be determined that a sufficient amount of tears for detection can be collected.

  The absorbent member 16 is made of a material capable of absorbing tears such as cellulose. The absorbing member 16 absorbs tear fluid and labeling reagent that have moved from the detecting member 14 by capillary action. In addition, after the tear fluid is collected, when the collection member 10 is immersed in a developing solution such as purified water to develop the tear fluid, the absorbing member 16 absorbs the developing solution, so that the developing solution is smoothly developed. That is, the absorbing member 16 has a function of preventing the backflow of the developing liquid. In addition, the absorbing member 16 also has a function of removing impurities washed away by the developing solution from the detecting member 14.

  The support member 18 is provided in contact with a sheet-like first support 18a that supports the detection member 14 and a surface opposite to the detection member 14 at the upstream and downstream ends of the support 18a. And a second support 18b composed of two parts spaced apart from each other. That is, the second support 18b is separated in the longitudinal direction.

  The first support 18 a has the same length and width as the detection member 14, and also has a function of backing the detection member 14. The first support 18a reinforces the structure of the strip 1 and prevents the strip 1 from dripping or bending during operation. The first support 18a is formed of a plastic film such as polyethylene terephthalate (PET), for example, and is liquid impermeable. The first support 18a can be formed by applying and drying a resin, which is a plastic film material, to the detection member 14. Alternatively, the detection member 14 can be formed by attaching a plastic film. Since the first support 18a is impermeable to liquid, the tear fluid in the detection member 14 moves in the longitudinal direction of the strip 1 in the detection member 14 without penetrating the first support 18a. Can do.

  The second support 18b is preferably an adhesive sheet having an adhesive surface, and may be transparent or non-transparent. The second support 18b is separated on the opposite side of the first support 18a from the detection member 14 so that it is preferably about 5 mm apart in the longitudinal direction. The second support 18b preferably extends 2 mm or more upstream from the background member 22.

  The second support 18b is preferably a liquid-impermeable adhesive sheet having an adhesive surface on the first support 18a side. The sampling member 10, the holding member 12, and the absorbing member 16 are bonded to the bonding surface. Since the second support 18b is impermeable to liquid, the tear fluid in the collecting member 10 and the holding member 12 does not penetrate into the second support 18b, and the longitudinal direction of the strip 1 passes through each member. Can be moved to. Further, there is no case where tears or a developing solution penetrates from the absorbent member 16 into the second support 18b and leaks to the outside of the strip 1 to stain the user's hand. Furthermore, since the second support 18b made of a liquid-impermeable material extends 2 mm or more upstream from the background member 22, the liquid can be prevented from penetrating into the background member 22. A specific example of the second support 18b is, for example, a plastic film (for example, a polyethylene terephthalate film) having an adhesive layer formed on the surface.

  Since the second support 18b is separated in the longitudinal direction, the length of the strip 1 can be easily adjusted by changing the arrangement pattern of the second support 18b, resulting in manufacturing variations. It is done. In addition, when the 2nd support body 18b isolate | separates except the part which overlaps with the 1st support body 18a, tears etc. may leak to the exterior of an apparatus.

  The first adhesive member 20a and the second adhesive member 20b are, for example, paper adhesive tapes. The first adhesive member 20a has an adhesive surface, on the downstream end of the sampling member 10 (that is, the non-projecting portion 10b), the holding member 12, and the upstream end of the detection member 14 on the adhesive surface. The end of the holding member 12 is bonded to the surface opposite to the support member 18 so that the end of the holding member 12 is fixed to the detection member 14. The first adhesive member 20a has a non-adhesive surface on the side opposite to the adhesive surface.

  The first adhesive member 20a adheres the sampling member 10, the holding member 12, and the detection member 14, thereby preventing these members from being separated from each other and increasing the strength of the strip 1. Further, the first adhesive member 20a covers the surfaces of these members, thereby preventing the tear fluid from volatilizing from these members and making it possible to obtain sufficient detection results with a smaller amount of collection. . Furthermore, the 1st adhesion member 20a accelerate | stimulates the movement by the capillary phenomenon of tears by coat | covering so that these members may be pressed down from the top. Further, the non-adhesive surface outside the strip 1 provides a handle (handle) portion when the user uses the strip 1 by hand.

  The second adhesive member 20b has an adhesive surface, on the surface opposite to the support member 18 of the detection member 14 and the absorption member 16 on the adhesive surface, and the upstream end of the absorption member 16 serves as the detection member. In addition to being bonded to each other, the downstream end of the detection member 14 and the downstream end of the absorbing member 16 and the second support 18b are bonded so as to be sandwiched from the downstream side. The second adhesive member 20b has a non-adhesive surface on the side opposite to the adhesive surface, and further has a knob portion 20b 'at the downstream end. The knob portion 20b 'is formed by bonding the second adhesive members 20b to each other at the folded portion of the second adhesive member 20b.

  The second adhesive member 20b adheres the detection member 14, the absorption member 16, and the second support 18b, thereby preventing them from being separated from each other and increasing the strength of the strip 1. In particular, the second adhesive member 20b can effectively strengthen the structure of the strip 1 by adhering them so as to be sandwiched from the downstream side. Further, the second adhesive member 20b covers the surface of the absorbing member 16 and has a non-adhesive surface on the outside, so that the user's hand used by picking the downstream end of the strip 1 with his / her hand is not stained. There is also an advantage that it can be completed. In particular, when the user uses the knob portion 20b ', it can be used more safely. That is, the second adhesive member 18 b functions as a handle (handle) portion of the strip 1.

  The background member 22 is affixed on the opposite side to the detection member 14 of the 1st support body 18a and the 2nd support body 18b. The background member 22 is, for example, a white paper tape (such as office sticker paper) having an adhesive surface on the support member 18 side. On the surface of the background member 22 opposite to the support member 18, marks indicating the positions of the detection reagent fixing part 14a and the control reagent fixing part 14b are colored.

  By providing a white background member, red capture color development by colloidal gold is emphasized. In addition, since the positions of the detection reagent fixing part 14a and the control reagent fixing part 14b are shown, it is easy to confirm the red line at each position. Further, since the background member 22 has an adhesive surface, the first support body 18a and the second support body 18b are bonded by the adhesive surface, and the structure of the strip 1 is strengthened.

  When the background member 22 is made of paper, if a liquid such as tears permeates the background member 22, the background may rise or the strip 1 may be drowned, making it difficult to confirm the captured color by the labeling reagent. The amount of tears that move to the detection member 14 may decrease, and a sufficient detection result may not be obtained. In order to make it difficult for such a situation to occur, it is preferable that the first support 18 a extends 2 mm or more upstream from the background member 22.

  As shown in FIG. 2, the second support 18b may not be separated. In this case, the structure of the strip 1 can be further strengthened. Moreover, as FIG. 2 shows, the 2nd adhesion member 20b may adhere | attach the background member 22 in the adhesion surface. In this case, after the background member 22 is bonded to the second support 18b, the second bonding member 20b is bonded.

  As shown in FIG. 3, the support member 18 may be composed of only one adhesive sheet.

  As illustrated in FIG. 4, the background member 22 may not be provided. In this case, it is preferable that the support member 18 has a function of enhancing capture color development by the labeling reagent. That is, it is preferable that the support member 18 is not transparent but has a color such as white, which facilitates visual confirmation of the captured color development.

  As shown in FIG. 5, the absorbing member 16 may extend so as to protrude from the downstream end of the support member 18 (adhesive sheet).

  As shown in FIG. 6, the sampling member 10 and the holding member 12 may not be integrated, and may be composed of separate fiber base materials. In this case, it is preferable that a part of the collecting member 10 and a part of the holding member 12 overlap each other. As a result, the structure is strengthened, and the tear fluid can easily move from the collection member 10 to the holding member 12 by capillary action. Further, the first adhesive member 20a and the second adhesive member 20b may not be provided as in the strip 1 of FIG.

  The strip 1 shown in FIG. 7 has the same configuration as the strip 1 of FIG. 1 except that the first adhesive member 20a and the second adhesive member 20b are not provided. Such a strip can also be mentioned as one embodiment of the strip obtained by the cutting method or the manufacturing method according to the present invention.

  For the strip 1 as described above, a laminate including a sheet-like support member 18 and a sampling member 10, a holding member 12, a detection member 14, and an absorption member 16 provided in contact with one surface of the support member 18 is prepared. And a manufacturing method including a step of cutting the laminate and cutting out the strip 1. Hereinafter, the case of manufacturing the strip 1 according to the embodiment of FIG.

The said laminated body can be produced through processes like the following (1)-(8), for example.
(1) Two 2 cm wide adhesive sheets “ARcare7815” (manufactured by Adhisive Research, trade name) cut to 21 cm are prepared. One of the adhesive sheets (hereinafter referred to as “adhesive sheet (A)”) is peeled off, and the adhesive sheet (A) with the adhesive surface exposed is placed on the work board so that the adhesive surface is on the top. Put. The adhesive sheet (A) corresponds to the upstream portion of the second support 18b in FIG.
(2) The detection reagent and the control reagent were immobilized on the membrane of a 2.5 cm-wide strip-shaped laminated sheet “SHF100225” (trade name, manufactured by Millipore), on which a porous membrane was provided on a support. A laminated sheet (hereinafter referred to as “laminated sheet (X)”) is prepared. The laminated sheet (X) cut to a length of 21 cm is arranged in parallel to the adhesive sheet (A) so as to overlap the adhesive surface of the adhesive sheet (A) by 14 mm. In the laminated sheet (X), the membrane corresponds to the detection member 14, and the support corresponds to the first support 18a.
(3) A sample pad containing a labeling reagent at one end of a 1.7 cm wide strip-shaped sample pad (product name “S14” manufactured by Whatman or product name “KS-40” manufactured by Enomoto Kogyo Co., Ltd.) (hereinafter “sample pad”) (Y) "). On the exposed surface of the adhesive sheet (A), a sample pad (Y) cut to a length of 21 cm is disposed so as to overlap the membrane by 1 mm. At this time, the sample pad is arranged in parallel with the membrane. The sample pad (Y) corresponds to the collecting member 10 and the holding member 12.
(4) A strip-shaped adhesive tape having a width of 13 mm (manufactured by Shamrock) is prepared in 21 cm, and affixed to a portion where the sample pad and the membrane overlap. At this time, the adhesive tape is arranged in parallel with the sample pad so that the adhesive tape and the membrane overlap each other by 3 mm. That is, the adhesive tape is applied so that the end of the sample pad is fixed to the membrane. This “adhesive tape” corresponds to the first adhesive member 20a.
(5) The backing paper of the other adhesive sheet (hereinafter referred to as “adhesive sheet (B)”) prepared in the above (1) is peeled off, and the adhesive surface is bonded to the membrane so as to overlap 5 mm. At this time, the adhesive sheet (B) and the membrane are made parallel. The adhesive sheet (B) corresponds to a downstream portion of the second support 18b.
(6) On the exposed surface of the adhesive sheet (B), a strip-shaped absorbent pad “CFSP173000” (trade name, manufactured by Millipore) having a width of 1.7 cm is cut into 21 cm. And 1 mm in parallel with the membrane. This absorbent pad corresponds to the absorbent member 16.
(7) 21 cm of a band-shaped adhesive tape (made by Shamrock) having a width of 50 mm is prepared and pasted so as to sandwich the absorbent pad and the adhesive sheet (B). At this time, the adhesive tape and the membrane are overlapped by 3 mm. That is, the adhesive tape is applied so that the end of the absorbent pad is fixed to the membrane. This adhesive tape corresponds to the second adhesive member 20b.
(8) A belt-shaped laser printer label (manufactured by A-One) having a width of 3.5 cm and a length of 21 cm is attached to the lower surfaces of the adhesive sheet (A), the membrane, and the adhesive sheet (B). At this time, the laser printer label is attached so that the end of the adhesive sheet (A) is exposed by 2 mm, and is arranged so as to be parallel to the adhesive sheet. This laser printer label corresponds to the background member 22.

  The strip 1 can be manufactured by cutting the laminated body thus produced into a strip having a certain width using, for example, a guillotine-type cutting machine. The width is not particularly specified, but the width is preferably 0.5 mm to 3 mm in order to obtain a strip suitable as a chromatographic strip for collecting a liquid sample directly from a living body.

  8 and 9 are schematic views showing an embodiment of a step of cutting the laminate with a guillotine-type cutting machine. FIG. 8 is a view as seen from a direction orthogonal to the moving direction of the laminated body, and FIG. 9 is a view as seen from the side from which the cut strip is fed out.

  The guillotine-type cutting machine 3 shown in FIGS. 8 and 9 has a movable blade 36 and a fixed blade 38 disposed so that the blade edges face each other, and a cutting edge of the fixed blade 38 disposed in the vicinity of the fixed blade 38. A table 30 having a work surface 31 in the same plane and a roller 32 facing the work surface 31 in the vicinity of the end of the table 30 on the fixed blade 38 side are provided.

  The movable blade 36 has a trapezoidal main surface, and is provided with the blade tip facing the fixed blade 38 side. The movable blade 36 can move in a direction approaching the fixed blade 38 and a direction away from the fixed blade 38. The fixed blade 38 has a rectangular main surface, and is fixed in a state where the blade tip faces the movable blade 36 side. The movable blade 36 and the fixed blade 38 are single blades having a blade edge formed by a blade surface 40 inclined with respect to each main surface and a flat back surface 42 on the back side thereof. The movable blade 36 is disposed so that the back surface 42 faces the base 30 side. The fixed blade 38 is arranged in such a direction that the blade surface 40 faces the base 30 side.

  On the work surface 31 of the table 30, the laminate 50 is placed with the mount 60 interposed therebetween. The laminated body 50 is arranged in a direction in which the background member 22 is on the work surface 31 side. That is, the mount 60 is stacked on the stacked body 50 on the opposite side of the stacked body 50 from the collection member 10, the holding member 12, the detection member 14, and the absorbing member 16. As the roller 32 rotates in the direction of the arrow 34, the stacked body 50 moves in the direction of the arrow 44 together with the mount 60. With the laminated body 50 and the mount 60 protruding from the tips of the movable blade 36 and the fixed blade 38 in a direction opposite to the base 30 with a predetermined width, the laminate 50 and the mount 60 are moved together with the mount 60 by the movable blade 36 and the fixed blade 30. By sandwiching, the strip 1 having a predetermined width W is cut out. The strip 1 can be continuously obtained from the laminated body 50 by sequentially cutting while moving the laminated body 50. The strip 1 is sent to the receiving unit 70 while dropping after being cut. For convenience of illustration, the base 30, the mount 60, and the laminated body 50 are drawn at intervals, but usually they are in contact with each other. The mount 60 and the laminated body 50 do not necessarily have to be bonded, and it is rather preferable from the viewpoint of workability and the like that they are simply stacked without bonding.

  The mount 60 is not particularly limited as long as it can be cut together with the laminated body 50, and a sheet-like paper material is preferably used. Examples of the sheet-like paper material include copy paper, filter paper, cardboard, cardboard paper, drawing paper, and wrapping paper. Among these, filter paper and copy paper are preferable. From the viewpoint of usability, the mount 60 preferably has substantially the same area as the laminated body 50. The thickness of the mount 60 is preferably 0.03 mm to 0.6 mm, more preferably 0.05 mm to 0.5 mm, and still more preferably 0.08 mm to 0.4 mm. By having the thickness of the range which the base_sheet | mounting_paper 60 concerns, the effect of suppression of defective-product generation | occurrence | production at the time of a base-sheet cutting is show | played notably.

  By stacking the mount 60 on the stack 50 on the side opposite to the movable blade 36 of the stack 50, the stack 50 is supported by the mount 60 and has a strength capable of resisting stress applied during cutting. That is, when cut, the stacked body 50 is less likely to be bent in the direction in which the stress is applied. Therefore, even when the laminated body 50 having a non-uniform thickness is cut into a thin strip shape, it is possible to obtain a good strip 1 in which the member is difficult to peel off. In addition, the cutting method according to the present embodiment does not require a special machine or operation, and does not even need to bond the laminate 50 and the mount 60. That is, according to the cutting method according to the present embodiment, the strip 1 having a good shape can be easily obtained.

  As shown in FIG. 10, the mount 61 may be further arranged not only on the base 30 side of the laminated body 50 but also on the side opposite to the base 30 of the laminated body 50, that is, on the member side such as the sampling member 10. In other words, a mount may be disposed on both sides of the laminate. Thereby, the effect which suppresses generation | occurrence | production of inferior goods is show | played more notably. The mount 61 may be the same as or different from the mount 60. For the same reason as the mount 60, the thickness of the mount 61 is preferably 0.03 mm to 0.6 mm, more preferably 0.03 mm to 0.1 mm, and still more preferably 0.04 mm to 0.07 mm. Further, only the mount 61 may be used without using the mount 60. However, in the case where the mount is disposed only on one side of the laminate, the mount is disposed on the side opposite to the sampling member or the like as in the embodiment of FIG. 8 in order to make the effect of the present invention remarkable. Is preferred.

  Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited to the following examples.

(Examination of strip width)
Using a product name “S14” manufactured by Whatman as a sample pad, a laminate was produced by the steps (1) to (8) described in the above embodiment. Under this laminate, a filter paper “3MM CHR” (product name, manufactured by Whatman) having a thickness of 0.34 mm is laid as a mount, and using a guillotine-type cutting machine shown in FIGS. 1 to produce a strip having the structure shown in FIG. The constant width (strip width) was set to 3 mm, 2.5 mm, 2 mm, 1.75 mm or 1.5 mm, and 30 strips were produced by cutting 30 times continuously in each case. The case where the strip width is set to 3 mm is Example 1, the case where the strip width is 2.5 mm is Example 2, the case where it is 2 mm is Example 3, and the case where it is 1.75 mm is Example 4. The case of 5 mm was taken as Example 5. Moreover, the case where it cut | disconnected without using a mount in Examples 1-5 was made into Comparative Examples 1-5, respectively.

Among the obtained strips, defective products such as those in which the members were peeled off or those cut after being cut and wound around the blade without being sent to the receiving part were seen. About each case of Examples 1-5 and Comparative Examples 1-5, the number of these inferior goods was counted, and the "defective rate" was calculated by the following formula. The results are shown in Table 1. In Table 1, “Peel” indicates the number of defective products from which the member has been peeled off, “Enrollment” indicates the number of defective products that are caught in the blade and cut again, and “Defect” indicates these Indicates the total number of sheets.
Defective rate (%) = (number of defective products / number of manufactured products (= 30)) × 100

(Examination of the type of mount)
In Example 5, a case where copy paper “α Eco Paper Type 80” (trade name, manufactured by Otsuka Shokai Co., Ltd.) having a thickness of 0.09 mm is used as Example 6 is used, and the number of defective products is similarly set. The defect rate was calculated by counting. The results are shown in Table 2.

(Examination of laminate)
In Example 5, when the steps (4) and (7) were omitted when the laminate was produced, that is, the case where no adhesive tape was used was Example 7, and the case where no mount was further used was Comparative Example 6. did. In Example 7 and Comparative Example 6, the strip 1 shown in FIG. 7 is manufactured. For Example 7 and Comparative Example 6 as well, the defect rate was calculated by counting the number of defects. The results are shown in Table 3.

  As shown in Table 1, the defect rates in Examples 1 to 5 were significantly lower than the defect rates in Comparative Examples 1 to 5. Therefore, when the strip shown in FIG. 1 having a width of 1.5 to 3 mm is manufactured, the occurrence of defects such as peeling of members is sufficiently caused by stacking the mount on the laminate and cutting the laminate together with the mount. It became clear that it can be suppressed.

  Further, as shown in Table 2, also in Example 6, the defect rate was sufficiently low. That is, it has been clarified that even when a copy sheet having a thickness of 0.09 mm is used as a mount, the effect of suppressing the occurrence of defects can be sufficiently obtained.

  Furthermore, as shown in Table 3, also in Example 7, the defect rate was sufficiently low. In other words, it has been clarified that the use of the mount can suppress the occurrence of defective products even when the laminate 1 produced without using the adhesive tape is cut to produce the strip 1 shown in FIG.

(Examination of mount layout)
Using a trade name “KS-40” manufactured by Enomoto Kogyo Co., Ltd. as a sample pad, a laminate was produced by the steps (1) to (8) described in the above embodiment. The mount is laid on the lower surface side (the other surface side is a plurality of members), the upper surface side (the plurality of members side) or both sides of the laminate, and constant using the guillotine-type cutting machine shown in FIGS. A strip having the configuration shown in FIG. 1 was produced by cutting to a width. The constant width (strip width) was set to 1.5 mm, and 30 strips were continuously cut to produce 30 strips. The case where the mount is disposed only on the lower side surface of the laminate is Example 6, the case where the mount is disposed only on the upper surface side of the laminate is Example 7, and the mount is placed on the lower surface side and the upper surface side of the laminate. The case where it was arranged was referred to as Example 8. Moreover, the case where the strip was manufactured similarly to Examples 6-8 except having not used the mount was set as Comparative Example 6.

As a mount to be disposed on the lower side surface of the laminate, a paper “Kinryo A” having a thickness of about 0.25 mm (trade name, principal components: cellulose, talc (chemical name: hydrous magnesium silicate [Mg ₃ Si ₄ O ₁₀ (OH) ₂ ]), calcium carbonate, starch). As a mount to be installed on the upper surface side of the laminate, a paper “lightweight thin-mouth PPC paper ST” (trade name, manufactured by Fuji Xerox) having a thickness of about 0.05 mm was used.

  About the obtained strip, the generation | occurrence | production state of inferior goods was evaluated similarly to the case of Examples 1-5 and Comparative Examples 1-5. The results are shown in Table 4.

  As shown in Table 4, the defect rates in Examples 6 to 8 were significantly lower than the defect rates in Comparative Example 6. Also from this result, when manufacturing a strip having a width of 1.5 mm shown in FIG. 1, by stacking the mount on one side or both sides of the laminate and cutting the laminate together with the mount, there is no problem such as peeling of members. It has become clear that the occurrence can be sufficiently suppressed.

  From the above experimental results, according to the cutting method and the manufacturing method according to the present invention, it is possible to sufficiently suppress the occurrence of defective products even when a laminated body having a non-uniform thickness is cut into a thin strip shape. It was confirmed that.

FIG. 6 is a side end view showing one embodiment of a strip. FIG. 6 is a side end view showing one embodiment of a strip. FIG. 6 is a side end view showing one embodiment of a strip. FIG. 6 is a side end view showing one embodiment of a strip. FIG. 6 is a side end view showing one embodiment of a strip. FIG. 6 is a side end view showing one embodiment of a strip. FIG. 6 is a side cross-sectional view showing an embodiment of a strip. It is a schematic diagram which shows one Embodiment of the process of cut | disconnecting a laminated body. It is a schematic diagram which shows one Embodiment of the process of cut | disconnecting a laminated body. It is a schematic diagram which shows one Embodiment of the process of cut | disconnecting a laminated body.

Explanation of symbols

  DESCRIPTION OF SYMBOLS 1 ... Strip, 3 ... Guillotine type cutting machine, 10 ... Collecting member, 10a ... Projection part, 10b ... Non-projection part, 12 ... Holding member, 14 ... Detection member, 14a ... Detection reagent fixing part, 14b ... Control reagent fixation Part, 14c ... nitrocellulose membrane (porous membrane), 16 ... absorbing member, 18 ... support member, 18a ... first support, 18b ... second support, 20a ... first adhesive member, 20b ... 2nd adhesive member, 20b '... knob part, 22 ... background member, 30 ... stand, 31 ... work surface, 32 ... roller, 34 ... arrow, 36 ... movable blade, 38 ... fixed blade, 40 ... blade surface, 42 ... back side, 44 ... arrow, 50 ... laminate, 60, 61 ... mount, 70 ... receiving part.

Claims (12)

A laminate having a sheet-like support member and a plurality of members provided in contact with one surface of the support member, the laminate having a portion where two or more of the plurality of members overlap each other and a portion not overlapping each other, A method of cutting so that the overlapping portion and the non-overlapping portion are cut together with the support member,
A method of disposing a mount on the other surface side of the support member and / or the plurality of members, and cutting the laminate together with the mount from the plurality of members.   The plurality of members are a plurality of strip-shaped members arranged in parallel to each other, and the laminate is cut so that a strip having a predetermined width is cut out along a direction orthogonal to the longitudinal direction thereof. The method of claim 1.   The method according to claim 2, wherein the predetermined width is 0.5 mm to 3 mm.   The method according to any one of claims 1 to 3, wherein the mount is a sheet-like paper material.   The method according to any one of claims 1 to 4, wherein the thickness of the mount is 0.03 mm to 0.6 mm.   The method according to claim 1, wherein the support member is liquid-impermeable.   The plurality of members include a member having a non-woven fabric, a member having a porous membrane, and a member formed of cellulose, which are arranged in this order on the support member. The method as described in any one of.   An adhesive member in which the laminated body is bonded to the surface of the two overlapping members opposite to the support member so that the end of one member is fixed to the other member. A method according to any one of claims 1 to 7, comprising.   The method as described in any one of Claims 1-8 which cut | disconnects the said laminated body with the single blade in which only the single side | surface of a blade edge | tip is inclined.   A movable blade and a fixed blade that are arranged so that the blade tips are opposed to each other, and the movable blade is a single blade with only one side of the blade tip inclined, and the laminate is cut using a guillotine-type cutting machine. The method as described in any one of 1-8.   The manufacturing method of a strip including the process of cut | disconnecting the said laminated body and cutting out a strip by the method as described in any one of Claims 1-10.   The manufacturing method according to claim 11, wherein the strip is a chromatographic strip for detecting a substance to be detected in a liquid sample by chromatography.

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