JP5159766B2 - Method for producing rumen protecting choline - Google Patents

Description

  The present invention relates to a method for producing ruminally protected choline (ruminal protected choline) that can be stably absorbed into the lower digestive tract because it is not decomposed by microorganisms, pH and moisture in the rumen. More specifically, (a) a step of producing choline immobilized on a carrier by immobilizing liquid concentrated choline on the carrier and then removing water from the immobilized choline; (b) A step of producing primary encapsulated choline by encapsulating choline immobilized on a carrier with a binding coating agent, and (c) a primary encapsulated choline having a size of 0.5 to 2.0 mm from the primary encapsulated choline. Screening, (d) the screened primary encapsulated choline was extremely cured A double-coated rumen-protecting choline comprising a step of secondary encapsulation with one or more selected from the group consisting of fats and oils (extremely hydrogenated oils and fats) and a fatty acid having a melting point of 40 ° C or higher It relates to a manufacturing method.

  In some livestock studies, choline is known to be an essential component, but the amount of choline required for lactating dairy cows has not yet been clarified, and in most ruminant ruminants Therefore, it is desired to develop a technique for protecting choline from its degradation in the rumen so that it can be absorbed and used in the small intestine through the rumen.

  The lactating response to choline is almost the same as the response to the essential amino acid methionine, and typical symptoms during choline deficiency include muscle weakness, fatty liver development, and renal bleeding.

  A study of injecting choline has confirmed that choline is a limiting nutrient in milking dairy cows. Fatty liver develops due to abnormalities in liver metabolism and secretion during choline deficiency. As a result, the amount of fatty acid transferred increases, and as a result, the occurrence of fatty liver decreases.

  Choline affects blood glucose, plasma cholesterol, blood insulin and serum NEFA and contributes significantly to improving milk production, milk composition, weight gain and conductor properties, but choline chloride supplemented by diet is ruminant It is reported that it is almost decomposed in the body.

  Ruminant microorganisms break down phosphatidylcholine in feed into choline and phosphodiglycerides, and rumen microorganisms break down choline by converting choline to methane via the intermediate metabolite trimethylamine. . Eventually, it is known that less than 1% of the choline in the feed reaches the small intestine.

  Choline is an essential nutrient for livestock animals, but for ruminants it is broken down in the rumen and its effectiveness is not recognized. Technology to prevent it has been developed.

  For example, an encapsulated choline composition (US 5,807,594) for increasing the weight of ruminants has been reported. However, due to the various raw materials used as the encapsulating agent for this choline composition, it appears to be quite expensive to actually apply to the product.

  On the other hand, an encapsulated choline compound to be fed to lactating dairy cows has been disclosed (US 5, 496, 571), which is decomposed in the rumen by applying the same physiologically active substance as choline via a hydrophobic coating. Although it is disclosed that it can be prevented (US 5, 190, 775), details of the manufacturing method are not disclosed. According to said patent, a simple coating or encapsulation with a hydrophobic substance is limited in that the active substance is completely protected in the rumen by the microorganisms of the rumen. There is.

  Also, WO94 / 15480 discloses that ruminants feed on an encapsulated choline composition for the purpose of increasing milk production. In this patent as well, a hydrophobic coating is used. The manufacturing method is not specifically disclosed. Further, WO96 / 08168 discloses that a liquid choline compound is encapsulated in a carrier and coated with a fatty acid or a fatty acid salt to produce a ruminant feed additive. When coating with salt, it has the disadvantage that it reacts with carbon dioxide in the air.

  In addition, lipid coatings (WO03 / 059088A1) and double coatings (US2005 / 0019413A1) that can stabilize choline in the rumen are disclosed, but have the disadvantage that the above coating formation method is complicated.

  As mentioned above, there have been numerous patent applications relating to choline compounds for weight gain and milk production, and most of these patents are commonly used with specific protective substances to prevent the degradation of choline in the rumen. Although mention is made of using choline as a coating, details of its production method are not disclosed.

  Moreover, among the ruminating protective choline products that are currently commercialized, there are products that are manufactured by a method in which choline is mixed with a protective substance and refrigerated and sprayed. In this case, the cost of the site and the equipment is high and it is used. Since extremely cold air having a cold air temperature of 60 ° C. below zero is used, the production cost of the product increases.

  In addition, because the product is produced by a method that instantaneously cools, huge holes (macro holes) may be formed in the product, and not only the operating conditions at the time of product production are complicated, Since protection cannot be performed for choline arranged on the surface of the obtained rumen-preserving choline product, there is a disadvantage that the effect is reduced.

  For the above reasons, there is an urgent need for technical development of a coated choline composition that is stable against rumen degradation and can be supplied to ruminants.

Accordingly, the present inventors have made intensive efforts to solve the problem that choline is decomposed in the ruminant stomach of ruminants, and as a result, choline is immobilized on a carrier and water is removed from the immobilized choline. Then, the rumen-protected choline obtained by first encapsulating the particles from which water has been removed with a hydrophobic substance and then secondarily encapsulating with an extremely hardened oil or fatty acid having a melting point of 40 ° C. or higher is obtained. In the rumen, it was found that the rumen was stably decomposed and absorbed by the lower digestive organs without being decomposed by microorganisms, pH and moisture, and the present invention was completed.
US Pat. No. 5,807,594 US Pat. No. 5,496,571 US Pat. No. 5,190,775 International Publication No. WO94 / 15480 International Publication No. WO96 / 08168 International Publication No. WO03 / 059088 US Patent Publication No. 2005/0019413

  The object of the present invention is to provide a process for the production of double-coated rumen protective choline which is not degraded in the rumen by microorganisms, pH and moisture.

  In order to achieve the above object, the present invention provides (a) fixing liquid-concentrated choline on a carrier, and then removing water from the immobilized choline to thereby remove the choline immobilized on the carrier. (B) encapsulating the choline immobilized on the carrier with a binding coating agent to produce primary encapsulated choline, (c) 0.5 to 2.0 mm from the primary encapsulated choline. Selecting a primary encapsulated choline having a size; and (d) any of the selected primary encapsulated choline selected from the group consisting of oils and fats (oil and fat) hardened and fatty acids having a melting point of 40 ° C. or higher. Or a secondary encapsulation with one or more, a method for producing a double-coated rumen protecting choline.

  Other features and implementations of the invention will become even more apparent from the following detailed description and claims.

The present invention provides a method for producing a double-coated rumen protecting choline, and the rumen protecting choline produced according to the present invention is not decomposed by microorganisms, pH and water in the rumen, so that Can be absorbed into the digestive tract, it can be used to breed ruminants, increase the utilization efficiency of ruminant nutrients, increase livestock productivity, and choline enriched functionality Enables production of livestock products.
Furthermore, the method for producing rumen-protecting choline according to the present invention is applied to protect various components from degradation by rumen, and can be used to produce various new feed additives.

  The present invention relates to a method for producing a double-coated rumen protecting choline that can be stably absorbed and transferred to the lower digestive tract because it is not degraded by microorganisms, pH and moisture in the rumen.

  In the present invention, in order to produce a double-coated rumen protecting choline, first, liquid concentrated choline is immobilized on a carrier, and then water is removed therefrom to produce choline immobilized on the carrier.

  Here, the liquid concentrated choline is preferably one or a mixture of two or more selected from the group consisting of choline chloride, choline dihydrogen citrate, tricholine citrate and choline bitartrate, and the carrier is It is preferably a water-insoluble carrier selected from the group consisting of silica, cellulose, starch and zeolite, and more preferably, the carrier is silica.

  Next, the binding coating agent is encapsulated with the choline immobilized on the produced carrier to produce primary encapsulated choline in the form of granules or needles. In the above-mentioned encapsulation, the immobilized choline and the binding coating agent are mixed and stirred with a mixer such as a ribbon mixer, a general stirrer, a homomixer or a V-shaped mixer, and then the mixture is extruded with an extruder or a spreader. It is done by passing the machine. Here, the choline and the binder coating agent are preferably mixed with each other in a weight ratio of 1: 0.1 to 2.0, and the pore diameter of the porous plate in the extruder is preferably 0.5 to 2.5 mm. The temperature of the barrel in the extruder is preferably maintained at 10 to 60 ° C.

  Furthermore, the bond coating agent is preferably one or a mixture of two or more selected from the group consisting of monovalent soaps, divalent soaps, and fatty acids having a melting point of 40 ° C. or higher. The monovalent soap is preferably sodium soap or potassium soap, and the divalent soap is selected from the group consisting of beeswax, PEG, surfactant, calcium stearate, zinc stearate and magnesium stearate. preferable. The fatty acid having a melting point of 40 ° C. or higher is preferably selected from the group consisting of hardened oil, extremely hardened oil, palmitic acid and stearic acid, and more preferably hardened oil.

After sorting the primary encapsulated choline having a size of 0.5 to 2.0 mm from the primary encapsulated choline, the oil (fat and fat) extremely hardened on the selected encapsulated choline and the melting point is 40 ° C. or more. Secondary-encapsulated rumen-protected choline can be obtained by secondary encapsulation with one or more selected from the group consisting of the above fatty acids.

The content of the double coating layer in the double-coated rumen protective choline finally obtained is preferably 15 to 55% (w / w) of the whole.

The double-coated rumen protective choline obtained according to the present invention has no risk of the formation of large pores, which has been pointed out as a drawback in conventional commercial products manufactured by the freezing jet method. There is an advantage that it can be obtained in a simple shape.

  In addition, in the case of a product manufactured by a freezing injection method, this product has the disadvantage that choline is located on the surface of the particle and the stability in the rumen is inferior, whereas the double coating manufactured by the present invention Already rumen-protected choline is subjected to a secondary coating process on the primary coating product containing choline, so there is no risk of choline being distributed on the surface of the particles, and most of the added choline can maintain stability. There is a merit. In addition, since the refrigerant used in the refrigeration injection method is not used, the manufacturing cost can be reduced, and the cost for the site and the equipment can be kept low, thereby reducing the production cost of the product. it can.

  If a ruminant type of choline is fed to a ruminant, it is difficult to expect the effect of choline feeding because most of the choline that is fed is broken down in the rumen. It will be wasted. For this reason, by using the ruminating protective choline according to the present invention for raising the stability of choline for raising livestock, it is possible to increase the utilization efficiency of ruminant nutrients and increase the productivity of ruminants, It is possible to develop a feed additive capable of producing functional livestock products by increasing the effectiveness of nutrients.

Examples Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are merely for explaining the present invention in more detail, and according to the gist of the present invention, the scope of the present invention is not limited to these examples. It will be obvious to those with ordinary knowledge.

Example 1 Production of Double-Coated Rumen-Protecting Choline Choline immobilized on a carrier by removing water from choline in which liquid concentrated choline is immobilized on a carrier such as silica, cellulose, starch, and zeolite. Manufactured.

  Next, a binding coating agent such as beeswax, PEG, or hardened oil is added to the choline immobilized on the carrier, and mixed and stirred in a mixer such as a ribbon mixer, a general stirrer, a homomixer, or a V-shaped mixer. After that, the primary encapsulated coated choline was produced by performing extrusion through an extruder such as an extruder or a plodder. Here, the temperature of the extrusion barrel of an extruder such as an extruder or a pudder was maintained at 10 to 60 ° C.

  After the primary encapsulated choline having a particle size of 0.5 to 2.0 mm is selected from the primary encapsulated choline, the oil and fat extremely cured by the selected primary encapsulated choline and the curing is 40 ° C. or higher. One or more fatty acids such as vegetable oil, hardened corn oil, hardened cottonseed oil, hardened peanut oil, hardened palm kernel oil, hardened palm oil, hardened palm stearin oil, hardened sunflower oil, hardened rapeseed oil, palmitic acid and stearic acid ( A secondary encapsulation step using a secondary encapsulation device such as a high speed mixer, ribbon mixer, ladyge mixer or fluidized bed processor to this mixture containing the primary encapsulated choline. To produce a double-coated rumen protecting choline.

Experimental example 1
After 5 kg of choline was mixed with 1 kg of liquid hydrogenated oil (primary encapsulating material: binding coating agent), the mixture was injected through a perforated plate having a pore diameter of 1.0 mm to produce a primary encapsulating material. . Thereafter, 1 kg of the produced primary capsule material is put into a high-speed mixer, and 670 g of any one of a liquid hydrogenated oil and a fatty acid having a melting point of 40 ° C. or higher, or a mixture thereof (secondary encapsulated material). Addition to produce a secondary encapsulated material. At this time, the content of the secondary encapsulation material was 40.1% with respect to the total amount of the secondary encapsulation material finally produced.

Experimental example 2
5 kg of choline was mixed with 1 kg of liquid hydrogenated oil (primary encapsulating substance: binding coating agent), and then the mixture was injected through a perforated plate having a diameter of 1.2 mm to produce a primary encapsulating substance. Thereafter, 1 kg of the produced primary encapsulated material is put into a high speed mixer, and 670 g of any one of a liquid hydrogenated oil and a fatty acid having a melting point of 40 ° C. or higher or a mixture thereof is used as a secondary encapsulating material. As a secondary encapsulated material. At this time, the content of the secondary encapsulation material was 40.1% with respect to the total amount of the secondary encapsulation material finally produced.

Experimental example 3
After mixing 5 kg of choline with 1 kg of liquid hydrogenated oil (primary encapsulating material: binding coating agent), the mixture was injected through a perforated plate having a pore size of 1.5 mm to produce a primary encapsulating material. Thereafter, 1 kg of the produced primary encapsulated material is put into a high speed mixer, and 670 g of any one of a liquid hydrogenated oil and a fatty acid having a melting point of 40 ° C. or higher or a mixture thereof is used as a secondary encapsulating material. As a secondary encapsulated material. At this time, the content of the secondary encapsulation material was 40.1% with respect to the total amount of the secondary encapsulation material finally produced.

Experimental Example 4
5 kg of choline was mixed with 1 kg of liquid hydrogenated oil (primary encapsulating material: binding coating agent), and then the mixture was injected through a perforated plate having a pore size of 2.0 mm to produce a primary encapsulating material. Thereafter, 1 kg of the produced primary capsule material is put into a high speed mixer, and 670 g of a liquid hydrogenated oil and a fatty acid having a melting point of 40 ° C. or more or a mixture thereof is used as a secondary encapsulation material. Addition to produce a secondary encapsulated material. At this time, the content of the secondary encapsulating material was 40.1% with respect to the total amount of the secondary encapsulating material finally produced.

Experimental Example 5
After mixing 5 kg of choline with 1 kg of monovalent soap (primary encapsulating material: binding coating agent), this mixture was injected through a perforated plate having a pore diameter of 1.0 mm to produce a primary encapsulating material. Thereafter, 1 kg of the produced primary encapsulated material is put into a high speed mixer, and 670 g of any one of a liquid hydrogenated oil and a fatty acid having a melting point of 40 ° C. or higher or a mixture thereof is used as a secondary encapsulating material. As a secondary encapsulated material. At this time, the content of the secondary encapsulation material was 40.1% with respect to the total amount of the secondary encapsulation material finally produced.

Experimental Example 6
After 5 kg of choline was mixed with 1 kg of PEG (primary encapsulating material: binding coating agent), the mixture was injected through a perforated plate having a pore diameter of 1.0 mm to produce a primary encapsulating material. Thereafter, 1 kg of the produced primary encapsulated material is put into a high speed mixer, and 670 g of any one of a liquid hydrogenated oil and a fatty acid having a melting point of 40 ° C. or higher or a mixture thereof is used as a secondary encapsulating material. As a secondary encapsulated material. At this time, the content of the secondary encapsulation material was 40.1% with respect to the total amount of the secondary encapsulation material finally produced.

Experimental Example 7
After mixing 5 kg of choline with 1 kg of Tween 80 (primary encapsulating substance: binding coating agent), the mixture was injected through a perforated plate having a pore diameter of 1.0 mm to produce a primary encapsulating substance. Thereafter, 1 kg of the produced primary encapsulated material is put into a high speed mixer, and 670 g of any one of a liquid hydrogenated oil and a fatty acid having a melting point of 40 ° C. or higher or a mixture thereof is used as a secondary encapsulating material. As a secondary encapsulated material. At this time, the content of the secondary encapsulation material was 40.1% with respect to the total amount of the secondary encapsulation material finally produced.

Experimental Example 8
After mixing 5 kg of choline with 1 kg of span 80 (primary encapsulating material: binding coating agent), the mixture was injected through a perforated plate having a pore diameter of 1.0 mm to produce a primary encapsulating material. Thereafter, 1 kg of the produced primary encapsulated material is put into a high speed mixer, and 670 g of any one of a liquid hydrogenated oil and a fatty acid having a melting point of 40 ° C. or higher or a mixture thereof is used as a secondary encapsulating material. As a secondary encapsulated material. At this time, the content of the secondary encapsulation material was 40.1% with respect to the total amount of the secondary encapsulation material finally produced.

Experimental Example 9
5 kg of choline was mixed with 1 kg of liquid hydrogenated oil (primary encapsulating material: binding coating agent), and then the mixture was injected through a perforated plate having a pore diameter of 1.2 mm to produce a primary encapsulating material. Thereafter, 1 kg of the produced primary encapsulating material is put into a high speed mixer, and any one of liquid hardened oil and fatty acid having a melting point of 40 ° C. or higher, or 500 g of a mixture thereof is used as a secondary encapsulating material. As a secondary encapsulated material. At this time, the content of the secondary encapsulation material was 33.3% with respect to the total amount of the secondary encapsulation material finally produced.

Experimental Example 10
5 kg of choline was mixed with 1 kg of liquid hydrogenated oil (primary encapsulating material: binding coating agent), and then the mixture was injected through a perforated plate having a pore diameter of 1.2 mm to produce a primary encapsulating material. Thereafter, 1 kg of the produced primary capsule material is put into a high speed mixer, and any one of liquid hardened oil and fatty acid having a melting point of 40 ° C. or higher, or a mixture thereof 350 g as a secondary encapsulation material. Addition to produce a secondary encapsulated material. At this time, the content of the secondary encapsulating substance was 25.9% with respect to the total amount of the secondary encapsulating substance finally produced.

Experimental Example 11
5 kg of choline was mixed with 1 kg of liquid hydrogenated oil (primary encapsulating material: binding coating agent), and then the mixture was injected through a perforated plate having a pore diameter of 1.2 mm to produce a primary encapsulating material. Thereafter, 1 kg of the produced primary encapsulating material is put into a high speed mixer, and any one of liquid hardened oil and fatty acid having a melting point of 40 ° C. or higher, or 250 g of a mixture thereof is used as a secondary encapsulating material. As a secondary encapsulated material. At this time, the content of the secondary encapsulation material was 20.2% with respect to the total amount of the secondary encapsulation material finally produced.

Experimental Example 12
After mixing 5 kg of choline with 1 kg of liquid hydrogenated oil (primary encapsulating material: binding coating agent), the mixture was injected through a perforated plate having a pore diameter of 1.2 mm to produce a primary encapsulating material. Thereafter, 1 kg of the produced primary encapsulating material is put into a high speed mixer, and 150 g of any one of a liquid hydrogenated oil and a fatty acid having a melting point of 40 ° C. or higher or a mixture thereof is used as a secondary encapsulating material. As a secondary encapsulated material. At this time, the content of the secondary encapsulating material was 13.0% with respect to the total amount of the secondary encapsulating material finally produced.

Experimental Example 13
After 5 kg of choline was mixed with 1 kg of liquid hydrogenated oil (primary encapsulating material: binding coating agent), the mixture was injected through a perforated plate having a diameter of 1.2 mm to produce a primary encapsulating material. . Thereafter, 1 kg of the produced primary capsule material is put into a high-speed mixer, and any one of liquid hydrogenated oil and fatty acid having a melting point of 40 ° C. or higher, or a mixture of 50 g thereof as a secondary encapsulation material. Addition to produce a secondary encapsulated material. At this time, the content of the secondary encapsulating substance was 4.8% with respect to the total amount of the secondary encapsulating substance finally produced.

Experimental Example 14
After mixing 4 kg of choline with 2 kg of liquid hydrogenated oil (primary encapsulating material: binding coating agent), the mixture was injected through a perforated plate having a pore size of 2.0 mm to produce a primary encapsulating material. Thereafter, 1 kg of the produced primary encapsulated material is put into a high speed mixer, and 670 g of any one of a liquid hydrogenated oil and a fatty acid having a melting point of 40 ° C. or higher or a mixture thereof is used as a secondary encapsulating material. As a secondary encapsulated material. At this time, the content of the secondary encapsulation material was 40.1% with respect to the total amount of the secondary encapsulation material finally produced.

Experimental Example 15
After 3 kg of choline was mixed with 3 kg of liquid hydrogenated oil (primary encapsulating material: binding coating agent), the mixture was injected through a perforated plate having a pore size of 2.0 mm to produce a primary encapsulating material. Thereafter, 1 kg of the produced primary encapsulated material is put into a high speed mixer, and 670 g of any one of a liquid hydrogenated oil and a fatty acid having a melting point of 40 ° C. or higher or a mixture thereof is used as a secondary encapsulating material. As a secondary encapsulated material. At this time, the content of the secondary encapsulation material was 40.1% with respect to the total amount of the secondary encapsulation material finally produced.

Example 2: In vitro loss rate of rumen-protected choline The in vitro loss rate for each of the double-coated choline prepared according to Experimental Examples 1 to 15 was determined according to the following method. The results are shown in Table 1.

  To measure the stability of double coated choline prepared according to Experimental Examples 1-15 in vitro, each of the manufactured rumen protecting choline was placed in a test tube and distilled water was added to 5% Then, the aqueous solution was stirred for 2 hours under a temperature condition of 40 ° C. ± 0.1 ° C. using a JEIO TECH MC-11 multiple stirrer water bath.

  The agitated aqueous solution was treated with Whatman paper NO. After filtering through 3 and collecting only the solid content, the solid content was dried at 45 ° C. ± 0.1 ° C. for 12 hours or more to completely remove the remaining water.

After the dried sample was cooled to room temperature in a SANPLA DRY KEEPER, 300 mg of the cooled sample was added to a solvent in which chloroform and methanol were mixed at a volume ratio of 1: 1 and completely dissolved. The content of choline was measured using a 5% K ₂ CrO ₄ solution as an indicator and a 0.1 NAgNO ₃ solution as a titration reagent.

  As a result, as shown in Table 1, there was almost no difference in in vitro loss rate due to changes in the pore diameter of the porous plate (Experimental Examples 1 to 4). As a result of different types of primary encapsulating materials, in vitro loss rate when using monovalent soap and divalent soap PEG as primary encapsulating material than when using Tween 80 or Span 80 Resulting in a lower in vitro loss rate when using liquid hardened oil than when using PEG, Tween 80 or Span 80 as monovalent and divalent soaps as primary encapsulating material. Experimental examples 5 to 8) in which

  As a result of varying the amount of the secondary encapsulating substance added, it was found that the in vitro loss rate decreased as the amount of the secondary encapsulating substance added increased (Experimental Examples 9 to 13).

  In addition, as a result of different ratios of choline and primary encapsulating substance, it was found that the higher the ratio of primary encapsulating substance content to choline, the lower the in vitro loss rate (Experimental Examples 14 and 15).

  As a result, in the production of the double-coated rumen protective choline according to the present invention, a liquid hardened oil is selected as the primary encapsulating substance, and the content of choline and the primary encapsulating substance is 1: 0.5 or A double coating manufactured by performing a primary encapsulation process using a mixture mixed at a ratio of 1: 1, and then performing a secondary encapsulation process by increasing the amount of secondary encapsulation material added. It can be seen that the in vitro loss rate of spent rumen protective choline is minimized.

Example 3 Ruminating Protective Choline Feeding Test In the test tube test of Example 2, the ruminant produced according to Experimental Example 15 with a low in vitro loss rate, a suitable product size and a high choline content. In vivo feeding tests were performed using gastroprotective choline.

  For the breeding test of dairy cows using rumen-protected choline, a fully mixed sample consisting of 10 samples of mid-lactating dairy cows mixed with a mixed sample and a crude sample is sufficient twice a day for every 12 hours. The amount of rumen protective choline (choline content 25%) was 40 g per head (10 g choline reference), which was additionally given when feeding the complete mixed sample. Milking is performed twice a day to measure milk yield, and milk samples are collected and milk components and body are analyzed using milk component and somatic cell analyzers (LactoScope®, MK2, Delta Instruments, The Netherlands). Cell number was measured.

  As shown in Table 2, when the rumen protective choline is fed to the cow, the daily milk production of the milking cow is 9.9.2 kg before feeding to 32.1 kg after feeding, as shown in Table 2. There was an increase of 9%, and the contents of milk fat, milk protein, lactose and total solids also increased by 7.9%, 10.4%, 12.7% and 10.1%, respectively. In addition, the somatic cell count decreased by 61.9% from 167000 / ml to 103000 / ml by ruminating choline protective choline feeding, and was effective in improving milk quality. The choline content was 44.85 mg / ml. It was confirmed that the production of choline-enriched milk was possible with an increase of about 48.8% from ml to 66.75 mg / ml.

  As described above in detail, the present invention provides a method for producing a double-coated rumen protecting choline, and the rumin protecting choline produced according to the present invention is not degraded by microorganisms, pH and moisture in the rumen. Can be stably absorbed into the lower digestive tract and can be absorbed, which can be used for breeding ruminants to increase the utilization efficiency of ruminant nutrients and increase the productivity of livestock, and choline Enables the production of enriched functional livestock products. Furthermore, the method for producing rumen-protecting choline according to the present invention is applied to protect various components from degradation by rumen, and can be used to produce various new feed additives.

  While specific portions of the subject matter of the present invention have been described in detail above, such specific descriptions are merely preferred embodiments for those having ordinary knowledge in the art, and thus the scope of the present invention. It is clear that is not limited. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (11)

A method for producing a double-coated rumen protecting choline comprising the following steps:
(A) a step of immobilizing concentrated liquid choline on a carrier and then removing moisture to produce choline immobilized on the carrier;
(B) Primary encapsulation by encapsulating choline and binding coating agent in a weight ratio of 1: 0.1 to 2.0 using liquid hydrogenated oil with choline immobilized on the carrier as a binding coating agent. Producing choline;
(C) selecting 0.5 to 2.0 mm primary encapsulated choline from the primary encapsulated choline; and (d) an oil and a melting point of the selected primary encapsulated choline extremely cured. The step of secondary encapsulating the content of the double coating layer at 15 to 55% (w / w) of the total by combining any one or two or more selected from the group consisting of fatty acids of 40 ° C. or higher .   2. The concentrated liquid choline is one or a mixture of two or more selected from the group consisting of choline chloride, choline dihydrogen citrate, tricholine citrate and choline bitartrate. A method for producing rumen protecting choline.   The method for producing rumen-protected choline according to claim 1, wherein the carrier is a water-insoluble carrier selected from the group consisting of silica, cellulose, starch, and zeolite.   The extremely hardened oil and fatty acid having a melting point of 40 ° C. or higher are hardened vegetable oil, hardened corn oil, hardened cottonseed oil, hardened peanut oil, hardened palm kernel oil, hardened palm oil, hardened palm stearin oil, hardened sunflower oil, hardened rapeseed oil. 2. The method for producing rumen protecting choline according to claim 1, wherein the method is selected from the group consisting of palmitic acid and stearic acid.   The method for producing rumen protecting choline according to claim 1, wherein the step (b) is mixed and stirred by a mixer and then encapsulated by an extruder. 6. The method for producing rumen protecting choline according to claim 5 , wherein the mixer is selected from the group consisting of a ribbon mixer, a general stirrer, a homomixer, and a V-shaped mixer. The method for producing rumen protecting choline according to claim 5 , wherein the extruder is an extruder or a plodder.   The method for producing rumen protecting choline according to claim 1, wherein the primary encapsulated choline is granular or needle-shaped. The method for producing rumen protecting choline according to claim 5 or 7 , wherein the size of the perforated plate in the extruder is 0.5 to 2.5 mm. The method for producing rumen protecting choline according to claim 7 , wherein the temperature of the extrusion barrel in the extruder is 10 to 60 ° C.   The rumen according to claim 1, wherein the step (d) is performed using a secondary encapsulation device selected from the group consisting of a high speed mixer, a ribbon mixer, a ladyge mixer and a fluidized bed processor. Method for producing protective choline.