CN1561979A - Micro capsule for choline chloride for ruminant stomach and its preparing method - Google Patents

Abstract

A through-rumen microsoftgel choleaevin as feed additive is prepared from the SiO2 carried choleaevin as core and the hydrogenated plant oil as shell through proportionally mixing, and granulating.

Description

Microcapsule of rumen bypass becholine and preparation method thereof

Technical field

The present invention relates to microcapsule of a kind of rumen bypass becholine and preparation method thereof.

Background technology

Microcapsule technology originates from the 1950's, is to utilize natural or synthetic macromolecular material to make the capsule material, capsule pericardiums such as solids or liquid object is wrapped up in form.The diameter of microcapsule generally micron to the millimeter scope in, can be divided into microsphere, microcapsule, nanocapsule and nanosphere, form by encapsulated material and coating material, be wrapped in inner material and generally be called active matter, activating agent, core material, inner phase or implant, coating material often is called wall, carrier, shell, coating or film, can be organic polymer, the hydrosol, sugar, wax, fat, metal or inorganic oxide etc.Microcapsule product is used widely at aspects such as medicine, agricultural and chemical industry, and particularly the drug microcapsule technology obtains fast development.

The development of microcapsule technology and products thereof mainly is that microcapsule product has following a plurality of characteristics:

(1) can improve the physical property of the material that is wrapped, it is solid-state that liquid state is changed into, changes weight or volume, makes article that good released state be arranged, thus the article that make be convenient to be utilized by the mankind, as the colour developing of the encapsulation in autochromy technology product.

(2) can improve Stability of Substance; make material avoid the influence of environment; protection has absorptive material, is widely used in laying hen as type vitamin A microcapsulesization, and iron phosphate can improve its flowability with the mixture with absorptive vitamin B group by micro encapsulation.

(3) may command discharges, and makes the release of active component in the microcapsule product can adopt immediately discharge, delay time regularly release or suitable releasing patterns such as long-acting release.The release of its core can be by mechanical means (adding broken, the friction of kneadding) or chemical method (dissolving of the effect of enzyme, solvent, water).

(4) reduce toxic and side effects, reduce the adverse effect that noxious substance causes environment, as alleviating gastrointestinal distress to the rate of release of digestive system by control after the drug microcapsuleizations such as ferrous sulfate, aspirin.

(5) shielding taste and abnormal smells from the patient reduces material toxicity, as be coated with by hard gelatin capsule or sugar cover up some drugs do not measure abnormal smells from the patient.

(6) abnormal smells from the patient sustained release, the typical use of perfume microcapsuleization.A lot of essence or spice such as Oleum menthae can prevent volatilization by micro encapsulation.

(7) sustained-release material enters external environment as required, inconsistent chemical compound is isolated, for example with behind radical initiator (as the benzoyl peroxide) micro encapsulation, mix with curable mylar, under general condition mylar can not solidify, after the initiator microcapsule destroyed, mylar can solidify but under given conditions.

Preparing capsular method has a lot, according to the character of medicine and capsule material, particle diameter, release performance and the targeting requirement of microcapsule, can select different Microencapsulation Method.Specifically can select Microencapsulation Method by understanding to the features such as application requirements of the layer of structure of the softgel shell performance of the formation step of the source of the character of core material, shell material, micro encapsulation medium, microcapsule, microcapsule, microcapsule, microcapsule.Mainly contain physical mechanical method, chemical method and physical-chemical process.

The physical mechanical method be meant with solid-state, liquid article gas phase micro encapsulation or solid-state between directly micro encapsulation form, mainly contain spray drying method, granulation, pan coating method etc. are wherein done commonly used with spray drying.Formed product such as quick disintegrate film, enteric coating, release membranes etc.This method is simply quick, and required cost is low, but that the micro encapsulation product quality has is to be strengthened.Chemical method is meant that utilization monomer or macromolecule in solution produce cyst membrane by polyreaction or condensation reaction and make microcapsule, often be divided into interface polycondensation, the crosslinking with radiation method, the needed solvent of this method mostly is organic solvent, and treatment conditions are higher, develop this type of product cost height, the specification requirement height.Physical-chemical process is meant that under certain condition core-clad material and capsule material form cenotype and separate out in liquid phase, be also referred to as phase separation method.Step can be divided into the dispersion of core-clad material, the adding of capsule material, and the curing of the deposition of capsule material and capsule material is one of main technique of drug microcapsuleization.But its needed cost height, the specification requirement height.

Summary of the invention

The purpose of this invention is to provide microcapsule of a kind of rumen bypass becholine and preparation method thereof.

The microcapsule of rumen bypass becholine is to be that the choline chloride of carrier is a capsule heartwood material with silicon dioxide, and hydrogenated vegetable oil is the capsule material, by 1: 1 mixed, and granulates through granulator and to form particulate method.

The step of the microcapsule preparation method of rumen bypass becholine is:

1) be that the choline chloride of carrier is a capsule heartwood material with silicon dioxide, hydrogenated vegetable oil is the capsule material, forms mixture by 1: 1 mixed;

2) said mixture is put into granulator, once granulate into microcapsule product less than 75 ℃, get final product in temperature.

Choline chloride can reduce at the deliquescence of air and the destruction of other nutritional labeling in to feedstuff behind micro encapsulation, is mainly used in the ruminant feed, preventing its degraded in cud, but can discharge absorption behind omasum.Can directly join in the milk cow forage concentrate, add very easy to use.

The specific embodiment

Choline (choline) is the vitamin B group class, is called vitamin B again ₄, it can promote liver fat to prevent fat to accumulate the undesired of liver with the form of lecithin; The unstable state methyl can be provided, be used for homocystine and be converted into methionine; It is the precursor of sympathetic nervous system pulse delivery agent acetylcholine, in milk cattle cultivating, choline is as a kind of important vitamines, it is a kind of essential nutritional labeling of milch cow, also be a kind of important methyl source of body, can produce influence greatly lipid metabolism, ketoacidosis incidence rate, immune function, breeding and the fertility performance etc. of milch cow.Therefore choline finds broad application in feed industry, and it is to add with the form of choline chloride in feedstuff.But choline chloride has bad smell, the strong hygroscopicity of tool, and other are supported one's family have destruction, should not be as the raw material of premix material, so the research of stable type choline chloride seems very important.Wang Chengzhang etc. (1997) have done the failure test of the choline chloride powder of anti-caking agent to vitamin, and effect is fine.Also there is the research report in external Japan, and its bag is reached more than 80% by effect.But pay attention to both at home and abroad more birds and nonruminant the research of stable type choline chloride.And also do not report for ruminant stable type choline chloride technology and products thereof is domestic.Because the rapid Degradation of rumen microorganism, tiring of dairy cow diet choline is very low, often shows as the choline deficiency in the high yield cow simultaneously, therefore for avoiding in degradation in cud, need carry out stable type research to choline chloride.We expect utilizing microcapsule technology, choline chloride is filmed or wrap by, by blocking it and extraneous contacting, reduce its destruction to other nutrient, reduce simultaneously or delay its degraded in cud, increase it and in abomasum, digest and assimilate, arrive our targeting target.

Because microcapsule technology relates to a lot of other fields, therefore need do the research work of a lot of aspects.According to the ultimate principle and the condition of microcapsule technology, we mainly are the selections of carrying out capsule-core raw material type, coating material and micro capsule technology method.

At first we have carried out the selection of choline chloride raw material according to the characteristic of choline chloride and mode of searching for food in ruminant and the digestion characteristics in the cud.China's feed industry circle is now main adopt some agricultural byproducts (corn cob, drum head, Testa oryzae etc.) although, stone powder and silicon dioxide etc. to choline chloride liquid adsorb, method such as oven dry handles. its performance has also had improvement, and its stability and result of use be ideal not really.As the 50% choline chloride additive product made from direct absorption of paddy skin and drying, very easily suction is easily lumpd, and mobile extreme difference, and other feed nutrition units such as vitamin are have serious destruction.But the most frequently used because corn cob is the choline chloride of carrier, we with regard to primary election its as core materials.

Secondly, we select coating material.Consider that from angle we have selected acrylic materials and hydrogenated vegetable oil as feed safety.

Be the selection of micro capsule technology and prescription again.Based on the angle of operability, safety, cost, what we mainly adopted is the method for physical mechanical.Prescription is to carry out according to domestic and international microcapsule prescription to medicine.

Be exactly the quality evaluation of considering microcapsule product at last.

We have carried out the making of micro capsule technology according to the requirement of above-mentioned several respects.At first we as coating material, to be that the choline chloride of carrier is a core materials with corn cob, have designed many different prescriptions with oils and fats, carry out microcapsule and operate.Micro capsule technology originally adopted the spraying coagulation, promptly earlier oils and fats was melted, and adopted the microcapsule method of fluidized-bed process again.This method is similar to spray drying method, and different is at the used air themperature of hothouse and the different in kind of used capsule material.It is 50-80 ℃ solid fat, cured, single acid anhydride fat etc. that the capsule material is selected some fusing points usually for use.The temperature of hothouse air is a room temperature or through cold treatment, far below the fusing point of capsule material.Core can be water miscible mineral, enzyme, water miscible little element, acidic flavoring agent, flavor substance powder etc. of giving birth to.With this method the capsule material of acidic flavoring agent with different melting points is mixed in proportion after the embedding respectively again, can when heating, discharges acidic flavoring agent in batches, cause the effect of " slow release ".Because greasy fusing point and freezing point are all very low, so the oils and fats of ejection is easy to spray gun is stopped up, this method can't be carried out but in operation.So we select other method; adopt granulator to granulate; made granule meets the external form condition of our needs; but when carrying out its quality evaluation; do not reach the effect that we need; by the granule of this method system, we adopt method-medicine leaching commonly used in the micro capsule technology to estimate.By measuring, we find the protection efficient of choline chloride maximum is not reached 50%.We have selected acrylic resin as coating material thus; with 92% spirit solvent dissolving acrylic resin, carry out microcapsule processing, adopt spray-dired fluidized-bed process method; when but we carried out quality evaluation to microcapsule product, protection efficient did not reach 50% yet.

Pass through the further analysis to core materials then, we think it may is the protective effect that the carrier of choline chloride has hindered coating material.Because the choline chloride feed granule that the corn cob material is made is very big, we have screened the choline chloride of many different carriers thus, selecting at last with silicon dioxide is the choline chloride of carrier, and processes with acrylic resin and oils and fats respectively with the method for fluidized-bed process.When carrying out quality evaluation, the highest 50% the protection effect that also can only reach.Its effect neither be very desirable.Simultaneously, greasy thawing need expend a large amount of power costs and a large amount of alcoholic solution of acrylic resin dissolving needs, and both required costs are also higher.

We are by anatomizing and summing up, and final decision continues the method that employing granulator is granulated.Through the first test result of small sample, we have designed the prescription of different proportion, different processing temperatures and processing number of times, and different granular sizes is processed.And it is carried out quality evaluation, last measurement result reaches more than 75% for protection efficient.Below its concrete operations are:

With content is that (silicon dioxide is carrier for 50% feed grade choline chloride, granularity is more than 40 orders) be capsule heartwood material, feed grade hydrogenated vegetable oil (granularity is about 12 orders) is pressed 1: 1 mixed for the capsule material, fully mixing, take the microcapsule method of granulating, produce once to granulate.The granulation maximum temperature can not be above 75 ℃.Made microcapsule product is microemulsion white, is the solid cylindrical granule, and average length is 2-3cm, and diameter is about 3.1mm.Product can directly join in the milk cow forage concentrate, adds very easy to use.

Claims (6)

1. the microcapsule of a rumen bypass becholine is characterized in that with silicon dioxide being that the choline chloride of carrier is a capsule heartwood material, and hydrogenated vegetable oil is the capsule material, by 1: 1 mixed, and granulates through granulator and to form particulate method.

2. want the microcapsule of 1 described a kind of rumen bypass becholine according to right, it is characterized in that the granularity of the said capsule heart is: 30～60 orders.

3. want the microcapsule of 1 described a kind of rumen bypass becholine according to right, it is characterized in that the granularity of said capsule material is: 10～30 orders.

4. want the microcapsule of 1 described a kind of rumen bypass becholine according to right, it is characterized in that the choline chloride content of said capsule heartwood material is: 50～90%.

5. the microcapsule preparation method of a rumen bypass becholine is characterized in that the step of method is:

1) be that the choline chloride of carrier is a capsule heartwood material with silicon dioxide, hydrogenated vegetable oil is the capsule material, forms mixture by 1: 1 mixed;

2) said mixture is put into granulator, once granulate into microcapsule product less than 75 ℃, get final product in temperature.

6. want the microcapsule preparation method of 5 described a kind of rumen bypass becholines according to right, it is characterized in that said microcapsule product is microemulsion white, be the solid cylindrical granule, average length is 1～3cm, and diameter is 3～3.3mm.