JP5132139B2 - External preparation for skin and food and drink - Google Patents

Description

  The present invention relates to an external preparation for skin and food and drink containing natural ingredients as active ingredients, and a cell activator, collagen production promoter, whitening agent, antioxidant and fat accumulation inhibitor containing natural ingredients as active ingredients.

  Various causes can be considered as causes of aging such as skin wrinkle formation, reduced elasticity, roughness, and rash. That is, the dermis and epidermis of the skin are composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin, collagen, and hyaluronic acid that are outside these cells and support the skin structure. In fresh skin, moisture retention, flexibility, elasticity and the like are ensured by maintaining the homeostasis of the interaction between these skin tissues. However, external factors such as ultraviolet rays, contaminated air, drying, excessive skin washing, and aging cause collagen and elastin degeneration / reduction and cell damage. As a result, the protective function and elasticity of the skin are reduced, the keratin begins to peel abnormally, the skin loses its elasticity and gloss, and exhibits aging symptoms.

  Melanin is mentioned as a factor of skin darkening. Melanin plays a role in protecting the body from ultraviolet rays in the skin, but due to local generation and uneven accumulation, pigmentation occurs, causing skin darkening, spots and freckles. In general, melanin changes from tyrosine to dopa, from dopa to dopaquinone by the action of the enzyme tyrosinase biosynthesized in pigment cells, and then formed through intermediates such as 5,6-dihydroxyindophenol. It is said that. Therefore, in order to prevent, ameliorate or treat pigment deposition, it is useful to inhibit the activity of tyrosinase involved in melanin production.

  In recent years, active oxygen has attracted attention as a factor that particularly oxidizes biological components, and its adverse effect on living organisms has become a problem. Reactive oxygen is essential in the sterilization mechanism of phagocytic cells and plays an important role in removing viruses and cancer cells. However, excessive production of active oxygen attacks in vivo molecules constituting membranes and tissues in the living body and induces various diseases. For example, active oxygen is considered to decompose biological tissues such as collagen, or to generate lipid peroxides that oxidize fats and oils and damage cells, and these disorders caused by active oxygen are It is also considered to cause aging such as skin wrinkle formation and reduced elasticity.

  In recent years, various diseases such as obesity and hyperlipidemia caused by excessive food intake, lack of exercise, stress, etc. have become a serious social problem. Various methods have been studied in order to prevent and improve the above. For example, dietary restriction and exercise methods, intake of dietary fiber, use of lipolysis promoters, etc. are mentioned, but these are mainly methods for reducing the fat already accumulated in the body, and as a fundamental improvement It was considered insufficient. On the other hand, the method for suppressing the accumulation of fat in the living body directly suppresses the accumulation of fat in the body, and thus is excellent in fundamental improvement of obesity and diseases, and is a daily preventive method. It is also effective.

Regarding the plants of the leguminous family (Degumosae) genus Desmodium (Desmodium triquetrum DC.) Or a topical skin preparation containing an extract thereof has been reported (see Patent Document 1), Amorseco ( There are no reports on external preparations for skin and foods and drinks containing an extract of Desmodium adscendens).
JP 2003-20122 A

  From the above, cell activation and collagen production promotion can contribute to the prevention and improvement of skin aging such as the formation of wrinkles on the skin and the decrease in elasticity. Further, inhibition of tyrosinase activity contributes to skin whitening. Furthermore, inhibition and suppression of active oxygen are useful, and it is considered that, for example, skin aging can be prevented or improved. Furthermore, suppression of adipocyte accumulation is considered useful for diseases such as obesity and hyperlipidemia.

  Therefore, the first object of the present invention is to find a cell activator from natural products, and to provide a cell activator comprising the same as an active ingredient.

  A second object of the present invention is to find a natural product that has a collagen production promoting action from natural products, and to provide a collagen production promoter that uses this as an active ingredient.

  A third object of the present invention is to find a natural product having an inhibitory effect on tyrosinase activity, and to provide a whitening agent using this as an active ingredient.

  A fourth object of the present invention is to find an active oxygen inhibitory / suppressing action from among natural products, and to provide an antioxidant containing the active ingredient as an active ingredient.

  In addition, a fifth object of the present invention is to provide a fat accumulation-inhibiting agent that has a fat accumulation-inhibiting action among natural products and uses it as an active ingredient.

  Furthermore, the present invention sixthly finds a natural product having a cell activation effect, a collagen production promoting effect, a whitening effect, an antioxidant effect or a fat accumulation inhibiting effect, and provides a skin external preparation containing the same. The purpose is to do.

  Furthermore, the present invention seventhly finds a natural product that has a cell activation effect, a collagen production promoting effect, a whitening effect, an antioxidant effect or a fat accumulation inhibiting effect, and provides a food or drink containing the same. For the purpose.

  The present inventors have studied various naturally-derived components applicable to skin external preparations and foods and drinks. As a result, we found an excellent cell activation, collagen production promoting, whitening, antioxidant and fat accumulation-inhibiting effects in extracts of leguminous (Leguminosae) plants belonging to the genus Desmodium (Desmodium adscendens) Further studies have been made and the present invention has been completed. That is, the present invention provides a cell activator, a collagen production promoter, a whitening agent, an antioxidant, and a fat accumulation inhibitor containing an Amorseco extract as an active ingredient. Furthermore, the skin external preparation and food-drinks containing the extract of Amor Seco are provided.

  According to the present invention, a cell activator, a collagen production promoter, a whitening agent, an antioxidant, and a fat accumulation inhibitor having excellent effects are provided. Moreover, according to this invention, the skin external preparation and food-drinks which have a cell activation effect | action, a collagen production promotion effect | action, a whitening effect | action, an antioxidant effect | action, and a fat accumulation suppression effect are provided.

  Hereinafter, the present invention will be described in detail. In the present invention, the “Amorseco extract” includes a case where Amorseco (Desmodium adscendens), which is a plant belonging to the genus Leguminosae, is used as it is as a raw material for extraction. The obtained extract, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or a crude product or a purified product thereof is preferably used.

  The constituent parts of the plant used for the extract of Amor Seco are not particularly limited, and constituent parts such as leaves, roots, seeds, and stems can be used as the extraction raw material. For convenient use, leaves, stems, and seeds may be used. From the viewpoint of effectiveness, leaves and stems may be used. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably in the range of about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but can be, for example, about 1 hour to 14 days.

  As extraction solvents, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethers such as ethyl ether and propyl ether, butyl acetate and Esters such as ethyl acetate, ketones such as acetone and ethyl methyl ketone, and solvents such as water can be used, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  The extract of Amorseco by the above solvent can be used as it is, but it can also be used by concentrating or concentrating to dryness and re-dissolving it in water or a polar solvent. In addition, the extract may be used after subjecting the extract to purification treatment such as decolorization, deodorization, and desalting, and fractionation treatment such as column chromatography. The extract of Amorseco, its processed product and its fraction can be freeze-dried after each processing and fractionation and dissolved in a solvent at the time of use.

  Amorseco extract has excellent cell activation, collagen production promotion, whitening, antioxidant and fat accumulation suppression, cell activator, collagen production promoter, whitening, antioxidant and fat accumulation It can be used as an inhibitor. The extract of Amorseco has a cell activation effect and a collagen production promotion effect, and is useful for various aging symptoms of the skin, for example. Therefore, Amorseco extract is also useful as an anti-aging agent.

  The whitening effect of the extract of Amorseco is exhibited based on the tyrosinase activity inhibitory action of melanocytes. However, the whitening action of the extract of Amorseco is not limited to the whitening action exhibited based on the above action.

  The antioxidant action of the extract of Amorseco is exhibited based on, for example, an active oxygen scavenging action and / or a radical scavenging action. However, the antioxidant action of the extract of Amorseco is not limited to the antioxidant action exhibited based on the above action. Here, “active oxygen” includes superoxide, hydrogen peroxide, hydroxy radical, singlet oxygen and the like. “Radical” means a molecule or atom having one or more unpaired electrons, and includes superoxide, hydroxy radical, DPPH and the like. In addition, since Amorseco extract has an active oxygen scavenging action and a radical scavenging action, it can also be used as an active ingredient of an active oxygen scavenger or a radical scavenger.

  Amorseco extract is useful for prevention and improvement of skin aging, has a whitening action and an antioxidation action, has a fat accumulation-inhibiting action, and has excellent usability and safety when applied to the skin. Therefore, it is suitable for blending into an external preparation for skin.

  The dosage form of the external preparation for skin to which the extract of Amorseco can be blended is not particularly limited. Specific examples thereof include a solubilizing system such as lotion, an emulsifying system such as cream and emulsion, a dispersion system such as calamine lotion, and a propellant. Aerosols, ointments, powders, granules and the like filled together. Specific examples of external preparations for skin include lotions, emulsions, creams, oils, pack body soaps, bath preparations, etc., and can be applied to hair, so shampoos, rinses, hair nicks, hair liquids, etc. Is mentioned.

  The amount of Amorseco extract to be added to the external preparation for skin can be adjusted depending on the type of skin external preparation, purpose of use, etc., but from the viewpoint of effect and stability, the total amount is 0.0001. -50.0 mass% is preferable, More preferably, it is 0.001-25.0 mass%.

  In addition to the Amorseco extract, the external preparation for skin that contains the Amorseco extract is usually incorporated into pharmaceuticals, quasi-drugs, skin cosmetics, hair cosmetics and cleansing agents as needed. Oily ingredients, moisturizers, powders, pigments, emulsifiers, solubilizers, detergents, UV absorbers, thickeners, drugs, fragrances, resins, antibacterial / antifungal agents, alcohols, etc. be able to. Moreover, in the range which does not impair the effect of this invention, combined use with another cell activator, a collagen production promoter, a whitening agent, an antioxidant, and a fat accumulation inhibitor is also possible.

  In addition, cell activators, collagen production promoters, whitening agents, antioxidants and fat accumulation inhibitors that contain Amorseco extract as an active ingredient should not only be applied to the skin and hair, but also taken orally. In addition, it can be applied to any food or drink or medicine. The food and drink here includes functional foods and nutritional supplements.

  Foods and drinks that can be blended with the extract of Amor Seco are not particularly limited, but specific examples include beverages such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks, and lactic acid drinks (concentrated concentrates and adjusting powders of these drinks) Ice cream, ice sherbet, shaved ice and other frozen desserts; buckwheat noodles, udon, harusame, gyoza skin, cucumber skin, Chinese noodles, instant noodles and other noodles; salmon, chewing gum, candy, gum, chocolate, tablet confectionery Confectionery such as snacks, biscuits, jelly, jam, cream, baked confectionery; fishery and livestock processed foods such as kamaboko, ham and sausage; dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise, Fats and processed foods such as shortening, whipped cream and dressing; seasonings such as sauces and sauces Soup, stew, salad, prepared foods, pickles, and the like.

  When the food or drink of the present invention is used as a dietary supplement or functional food, its form is not particularly limited. For example, protein (a protein source is a milk protein with high amino acid balance, soy protein, egg albumin, etc. with an amino acid balance) Protein is most widely used, but in addition to these degradation products, egg white oligopeptide, soybean hydrolyzate, etc., a mixture of amino acids alone is also used), sugars, fats, trace elements, vitamins, emulsifiers, Processing forms such as natural liquid foods, semi-digested nutritional foods and component nutritional foods, drinks, capsules and the like in which fragrances are blended may be used. When serving as sports drinks or nutritional drinks, nutritional additives and sweeteners such as easily digestible hydrous carbon, amino acids, vitamins, minerals, etc. are used to improve nutritional balance and improve the flavor when ingested. , Spices, fragrances, pigments and the like can also be blended.

  The form of the dietary supplement or functional food of the present invention is not limited to these, and may be the form of the above-mentioned general food or drink, but preferably in unit dosage form if possible.

  The cell activator, collagen production promoter, whitening agent, antioxidant, fat accumulation inhibitor, external preparation for skin, and food and beverage of the present invention described above are preferably applied to humans, As long as each effect is exhibited, it can also be applied to animals other than humans.

  Hereinafter, production examples of the extract of Amorseco and tests for evaluating each action will be described in more detail, but the technical scope of the present invention is not limited thereto.

[Production Example 1]
The leaves of Amor Seco were dried and pulverized. A 50% by mass aqueous ethanol solution 20 times the mass of the pulverized product was added, extracted for 2 hours while stirring at room temperature, and then insolubles were removed by filtration. And what was freeze-dried after concentration under reduced pressure was used as an Amor Seco extract of manufacture example 1.

[Production Example 2]
The leaves of Amor Seco were dried and pulverized, and purified water having an amount 20 times the mass of the pulverized product was added, followed by extraction by heating at 120 ° C. for 20 minutes in an autoclave. The insoluble material was removed by suction filtration while maintaining a high temperature state, and then freeze-dried and used as the Amorseco extract of Production Example 2.

[Evaluation of action]
1. Evaluation of human dermal fibroblast activation action Evaluation of the dermal fibroblast activation action of Amorseco extract was performed according to the following procedure. As a sample, the Amorseco extract produced in Production Example 1 was used.

Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% by weight fetal bovine serum (FBS). After culturing for 24 hours, the medium was replaced with 1% by mass of FBS-added DMEM medium to which samples having respective concentrations shown in Table 1 were added, and further cultured for 48 hours. Subsequently, the medium containing 400 mg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was exchanged and cultured for 2 hours. Formazan produced by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in Table 1 as relative values with the cell activation effect in the control with no sample added being taken as 100.

表１に示す結果より、アモールセコ抽出物を添加した培地を用いた場合、コントロールと比較して濃度依存的に優れた真皮線維芽細胞賦活作用が認められた。このことから、アモールセコ抽出物は、優れた真皮線維芽細胞賦活作用を有すること、ひいては抗老化作用を有することがわかった。

From the results shown in Table 1, when the medium supplemented with Amorseco extract was used, an excellent dermal fibroblast activation effect was observed in a concentration-dependent manner as compared with the control. From this, it was found that the Amorseco extract has an excellent dermal fibroblast activation effect, and thus has an anti-aging effect.

2. Evaluation of human epidermal cell activation effect The evaluation of the human epidermal cell activation effect of Amorseco extract was performed by the following method. As a sample, the Amorseco extract produced in Production Example 2 was used.

Human epidermis unwhelmed cells were seeded in a 96-well microplate so that there were 2.0 × 10 ⁴ cells per well. As the seeding medium, Dulbecco's modified Eagle medium (DMEM) to which 5% by mass of fetal bovine serum was added was used. After culturing for 24 hours, the medium was replaced with a 5% by mass FBS-added DMEM medium to which samples were added at respective concentrations shown in Table 2, and further cultured for 24 hours. Subsequently, the medium containing 100 mg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was replaced and cultured for 2 hours. Formazan generated by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in Table 2 as relative values with the cell activation effect in the control with no sample added being taken as 100.

表２に示す結果より、アモールセコ抽出物を添加した培地を用いた場合、コントロールと比較して濃度依存的に優れた表皮細胞賦活作用を有すること、ひいては抗老化作用を有することがわかった。

From the results shown in Table 2, it was found that when a medium supplemented with Amorseco extract was used, it had an excellent epidermal cell activation effect in a concentration-dependent manner compared to the control, and thus had an anti-aging effect.

3. Evaluation of human dermal fibroblast collagen production promoting action Evaluation of human dermal fibroblast collagen production promoting action of Amorseco extract was performed by the following method. As a sample, the Amorseco extract produced in Production Example 1 was used.

Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. As a seeding medium, 5% by mass of fetal bovine serum (FBS) was added to Dulbecco's modified Eagle medium (DMEM). After 24 hours, the medium was replaced with 0.5% by mass FBS-added DMEM medium to which samples were added at respective concentrations shown in Table 3, and further cultured for 24 hours. The ELISA method was used to quantify type I collagen secreted into the culture supernatant, and finally 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) against labeled peroxidase. ) And hydrogen peroxide were added and reacted, and the absorbance at 405 nm was measured with a microplate reader. The amount of protein was measured with a BCA Protein Assay Kit manufactured by PIERCE, and the amount of type I collagen produced per unit protein was determined. The evaluation results are shown in Table 3 as relative values when the amount of type I collagen produced per unit protein amount in the control with no sample added is defined as 100.

表３に示す結果より、アモールセコ抽出物を添加した培地を用いた場合、コントロールと比較して濃度依存的に優れた真皮線維芽細胞コラーゲン産生促進作用を有すること、ひいては抗老化作用を有することがわかった。

From the results shown in Table 3, it can be seen that when a medium supplemented with Amorseco extract is used, it has a superior dermal fibroblast collagen production promoting effect in a concentration-dependent manner compared to the control, and thus has an anti-aging effect. all right.

4). Evaluation of Human Epidermal Cell Collagen Production Promoting Action The Amorseco extract was evaluated for human epidermal untotalized cell collagen production promoting action by the following method. As a sample, the Amorseco extract produced in Production Example 2 was used.

Human epidermis unwhelmed cells were seeded in a 96-well microplate so as to be 2.0 × 10 ⁴ cells per well. As a seeding medium, 5% by mass of fetal bovine serum (FBS) was added to Dulbecco's modified Eagle medium (DMEM). After 24 hours, the medium was replaced with 5% by mass FBS-added DMEM medium to which samples were added at respective concentrations shown in Table 4, and further cultured for 5 days. For the quantification of type IV collagen secreted into the culture supernatant, a sandwich ELISA method using a monoclonal antibody against IV collagen (recognition site: α2 chain) and a biotinylated polyclonal antibody was used, avidinized horseradish peroxidase was added, The color was developed with 3,3 ′, 5,5′-tetramethylbenzine, and the absorbance at 650 nm was measured with a microplate reader. The amount of protein was measured with a BCA Protein Assay Kit manufactured by PIERCE, and the amount of type I collagen produced per unit protein was determined. The evaluation results are shown in Table 4 as relative values when the production amount of type I collagen per unit protein amount in the control with no sample added is 100.

表４に示す結果より、アモールセコ抽出物を添加した培地を用いた場合、コントロールと比較して濃度依存的に優れた表皮細胞コラーゲン産生促進作用を有すること、ひいては抗老化作用を有することがわかった。

From the results shown in Table 4, it was found that when a medium supplemented with Amorseco extract was used, it had an excellent epidermal cell collagen production promoting effect in a concentration-dependent manner compared to the control, and thus had an anti-aging effect. .

5. Evaluation of human epidermal melanocyte tyrosinase activity inhibitory activity Evaluation of the human epidermal melanocyte tyrosinase activity inhibitory activity of Amorseco extract was carried out by the following method. As a sample, the Amorseco extract produced in Production Example 1 was used.

Normal human epidermal melanocytes were seeded in a 96-well microplate at 3.0 × 10 ⁴ cells per well. Medium 154S was used as the seeding medium. After 24 hours, the medium was replaced with Medium 154S to which a sample was added at each concentration shown in Table 5, and the cells were further cultured for 48 hours. Next, the solution was replaced with 75 ml of a phosphate buffer containing 1% by mass of Triton-X to completely lyse the cells, and 50 ml of the solution was used as a crude enzyme solution. To the crude enzyme solution, 50 ml of 0.05% by mass L-dopa-containing phosphate buffer as a substrate was added and allowed to stand at 37 ° C. for 2 hours. The absorbance at 405 nm was measured immediately after addition of the substrate and at the end of the reaction with a microplate reader, and the amount of dopamelanin produced was determined by introducing the difference between the two measured values into the following equation.

Dopamelanin production = {(405 nm value after reaction-405 nm value before reaction)-2.166} / 5.238
In addition, the amount of protein was measured with a BCA Protein Assay Kit manufactured by PIERCE, and the amount of melanin produced per unit protein amount was determined. The evaluation results are shown in Table 5 as relative values when the melanin production amount per unit protein amount in the control with no sample added is defined as 100.

表５に示す結果より、アモールセコ抽出物を添加した培地を用いた場合、コントロールと比較して濃度依存的にメラニン生成量が抑制されていること、ひいてはチロシナーゼ活性阻害作用を有することがわかった。

From the results shown in Table 5, it was found that when a medium supplemented with Amorseco extract was used, the amount of melanin produced was suppressed in a concentration-dependent manner as compared with the control, and consequently, it had an inhibitory effect on tyrosinase activity.

6). Evaluation of DPPH radical scavenging ability DPMO radical scavenging ability of Amorseco extract was evaluated by the following method. As a sample, the Amorseco extract produced in Production Example 1 was used.

  100 ml of a 50 mass% ethanol aqueous solution to which the sample was added so as to have each concentration shown in Table 6 was added to a 96-well microplate. Thereto, 100 ml of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) ethanol solution was added and mixed well, and then allowed to stand at room temperature in the dark for 24 hours. Finally, the absorbance at 516 nm derived from the DPPH radical was measured. The extinction rate of DPPH radical is defined by the following equation, where (A) is the absorbance when the sample is not added and (B) is the absorbance when the sample is added.

Radical scavenging rate = {1- (B) / (A)} × 100
Table 6 shows the radical scavenging rate determined by the above equation.

表６に示す結果より、アモールセコ抽出物はDPPHラジカル消去作用を有すること、ひいては抗酸化作用を有するということがわかった。

From the results shown in Table 6, it was found that the Amorseco extract has a DPPH radical scavenging action, and thus has an antioxidant action.

7). Evaluation of neutral fat accumulation inhibitory action using normal human preadipocytes The neutral fat accumulation inhibitory action of Amorseco extract was evaluated by the following method. As a sample, the Amorseco extract produced in Production Example 1 was used.

Subcutaneous fat-derived normal human preadipocytes Cryo HPRAD-SQ (manufactured by Sanko Junyaku Co., Ltd.) were seeded in a 96-well microplate so as to be 1.0 × 10 ⁴ cells per well. PGM medium (containing 10% by mass FBS, 2 mM L-glutamine, 100 units / mL penicillin, 100 μg / mL streptomycin) was used as the seeding medium. Immediately before the cells become confluent, the medium is changed to PGM-differentiation medium (containing 10 μg / mL insulin, 1 μM dexamethasone, 200 μM indomethacin, 500 μM isobutyl-methylxanthine) to which the concentration is as shown in Table 7 and fat is added. Differentiation into cells was performed. After the induction of differentiation, the cells were cultured for 10 to 14 days until the control group (without sample addition) matured and a large number of lipid droplets accumulated in the cells. After the cells were collected, the cells were fixed using a 10% by mass neutral buffered formaldehyde solution. After washing with PBS (−), 0.5 mass / volume% oil red solution was added, and the mixture was cultured at 37 ° C. for 2 hours. After washing with PBS (−), methanol was added to extract the dye. Absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the amount of triglyceride accumulated was evaluated using the difference between the two measured values. In the evaluation, the relative value when the amount of accumulated fat in the control group was set to 100 was obtained. Table 7 shows the results.

表７に示す結果より、アモールセコ抽出物によると、中性脂肪の蓄積が抑制されることがわかった。したがって、アモールセコ抽出物は、抗肥満作用、痩身作用に寄与しうる。

From the results shown in Table 7, it was found that accumulation of neutral fat was suppressed according to the Amorseco extract. Therefore, Amorseco extract can contribute to anti-obesity action and slimming action.

  Then, the prescription example of the skin external preparation which mix | blended the Amorseco extract based on this invention is shown. In the following formulation examples, the amount of each component is expressed in mass%.

<Formulation Example 1: Skin Lotion>
(1) Glycerol 10.0, (2) Sodium lactate 0.5, (3) Polyoxyethylene hydrogenated castor oil (60E.O.) 0.2, (4) Amorseco extract (extract according to Production Example 1) 0.2, (5) Purification Water residue (Production method) Components (1) to (4) were mixed and homogenized, and then (5) was added and stirred uniformly to obtain a skin lotion.

<Formulation Example 2: Skin Lotion>
(1) Ethanol 10.0, (2) 1% by weight hydroxyethyl cellulose aqueous solution 20.0, (3) Amorseco extract (extract according to Production Example 2) 0.1, (4) Glycerin 7.0, (5) Sodium guaiazulene sulfonate 0.5, (6 ) Purified water residue (Production method) After mixing (1) to (6), the mixture was made uniform to obtain a lotion for skin.

<Prescription Example 3: Essence>
Oil phase components: (1) squalane 5.0, (2) white petrolatum 2.0, (3) beeswax 0.5, (4) sorbitan sesquioleate 0.8, (5) polyoxyethylene oleyl ether (20E.O.) 1.2,
Aqueous phase component: (6) Propylene glycol 5.0, (7) Residual water with 100 as a whole, (8) 1% by weight carboxyvinyl polymer aqueous solution 20.0, (9) Amorseco extract (extract according to Production Example 1) 0.5 , (10) 10% by weight potassium hydroxide aqueous solution 1.0, (11) ethanol 5.0, (12) perfume 0.2
(Production Method) The oil phase components (1) to (5) were mixed and heated to 75 ° C. to dissolve and homogenize. On the other hand, the aqueous phase components (6) to (8) were mixed and dissolved, heated to 75 ° C., the oil phase component was added and pre-emulsified, the pH was adjusted by adding (10), Emulsified with a mixer. After cooling, (11), (9) and (12) were added and mixed at 40 ° C. to obtain a serum.

<Formulation Example 4: Emulsion for skin>
Oil phase components: (1) stearic acid 0.2, (2) cetanol 1.5, (3) petrolatum 3.0, (4) liquid paraffin 7.0, (5) polyoxyethylene (10E.O.) monooleate 1.5, (6 ) Lactic acid bacteria extract 0.5
Aqueous phase components: (7) glycerin 5.0, (8) triethanolamine 0.1, (9) amorseco extract (extract according to production example 1) 0.3, (10) purified water residue (production method) (1) to (6) The oil phase components were mixed and heated to dissolve uniformly, and kept at 70 ° C. On the other hand, the aqueous phase components (7) to (8) and (10) were mixed and heated to be uniform to 70 ° C. The oil phase component was gradually added to the aqueous phase component while stirring to emulsify, and after cooling, (9) was added at 45 ° C. to obtain a skin emulsion.

<Prescription Example 5: Gel for skin>
(1) 1% by weight carboxyvinyl polymer aqueous solution 50.0, (2) polyoxyethylene hydrogenated castor oil (50E.O.) 0.5, (3) Amorseco extract (extract according to Production Example 1) 0.5, (4) dipropylene Glycol 8.0, (5) 10 mass% potassium hydroxide aqueous solution 1.0, (6) Purified water residue (Manufacturing method) (1) To which (2) to (4) were uniformly dissolved was added and stirred uniformly. An aqueous solution in which (5) was dissolved in (6) was added thereto to increase the viscosity to obtain a gel for skin.

<Formulation Example 6: Cream for skin>
Oil phase components: (1) beeswax 6.0, (2) cetanol 1.5, (3) reduced lanolin 8.0, (4) squalane 29.5, (5) lipophilic monostearate glyceride 4.0, (6) polyoxyethylene sorbitan monolaurate (20E.O.) 5.0
Water phase component: (7) Propylene glycol 5.0, (8) Amorseco extract (extract according to Production Example 1) 0.5, (9) Purified water residue (Production method) Oil phase components of (1) to (6) are mixed, Dissolved and heated to 75 ° C. On the other hand, the aqueous phase components (7) and (9) were mixed, dissolved, and heated to 75 ° C. Next, the oil phase component was added to the water phase component and pre-emulsified, and then uniformly emulsified with a homomixer, cooled and then brought to 45 ° C., and (8) was added to obtain a skin cream.

<Prescription Example 7: Oil-in-water emulsion ointment>
Oil phase components: (1) white petrolatum 25.0, (2) stearyl alcohol 25.0, (3) glycerin 10.0, (4) sodium lauryl sulfate 1.0
Aqueous phase component: (5) Amorseco extract (extract according to Production Example 2) 0.5, (6) Purified water residue (Production method) Mix and dissolve the oil phase components of (1) to (4) to make uniform 75 Heat to ° C. The oil phase component was added to (6) heated to 75 ° C. to emulsify, and after cooling, the temperature was changed to 45 ° C. and (5) was added to obtain an oil-in-water emulsion ointment.

<Prescription Example 8: Lotion>
(1) Ethanol 10.0, (2) 1,3-butylene glycol 5.0, (3) Amorseco extract (extract according to Production Example 1) 10.0, (4) Dipotassium glycyrrhizinate, (5) Fragrance 0.1, (6) Polyoxyethylene hydrogenated castor oil (60E.O.) 0.2, (7) Purified water residue (Preparation method) Dissolve (1), (2), (6), (5) uniformly, then dissolve (4) (7) was added, and (3) was added and mixed and dissolved uniformly to obtain a skin lotion.

<Prescription Example 9: Makeup Base Cream>
Oil phase components: (1) stearic acid 1.2, (2) cetanol 2.0, (3) glyceryl tri-2-ethylhexanoate 2.5, (4) self-emulsifying monostearate glyceride 2.0
Aqueous phase components: (5) Propylene glycol 10.0, (6) 10% by weight potassium hydroxide aqueous solution 1.5, (7) Amorseco extract (extract according to Production Example 1) 0.3, (8) Purified water Pigment components, etc .: (9) Titanium oxide 1.0, (10) Bengala 0.1, (11) Yellow iron oxide 0.4, (12) Fragrance 0.1
(Production Method) The oil phase components (1) to (4) were mixed and heated to 75 ° C. to make it uniform. On the other hand, the components (5), (6) and (8) are mixed, heated and dissolved at 75 ° C. to make it uniform, and the pigments (9) to (11) are added to this and homogenized with a homomixer. To obtain an aqueous phase component. The oil phase component was added to the water phase component, emulsified with a homomixer, cooled, and the components (12) and (7) were added and mixed at 40 ° C. to obtain a makeup base cream.

<Prescription Example 10: Milky Foundation>
Oil phase components: (1) stearic acid 2.0, (2) squalane 5.0, (3) octyldodecyl myristate 5.0, (4) cetanol 1.0, (5) monostearate glyceride 1.0
Aqueous phase components: (6) 1,3-butylene glycol 8.0, (7) 10% by mass potassium hydroxide aqueous solution 1.0, (8) Amorseco extract (extract according to Production Example 1) 0.3, (9) Purified water Residues set to 100, etc .: (10) Titanium oxide 9.0, (11) Talc 7.4, (12) Bengala 0.5, (13) Yellow iron oxide 1.1, (14) Black iron oxide 0.1, (15) Fragrance 0.1
(Production Method) The oil phase components (1) to (5) were mixed and heated to 75 ° C. to make it uniform. On the other hand, the aqueous phase components (6), (7), and (9) are mixed, heated and dissolved at 75 ° C. to make uniform, and the pigments (10) to (14) are added to this and added to the homomixer. The mixture was cooled after being uniformly dispersed, and (15) and (8) were added at 40 ° C. to obtain an emulsion foundation.

<Prescription Example 11: Hand cream>
Oil phase components: cetanol 1.5, (2) petrolatum 2.0, (3) liquid paraffin 10.0, (4) monostearate glyceride 1.5, (5) polysoxyethylene glyceryl isostearate (60E.O.) 2.5, (6) acetic acid Tocopherol 0.5
Aqueous phase components: (7) Glycerin 20.0, (8) Methyl paraoxybenzoate 0.1, (9) Amorseco extract (extract according to Production Example 1) 0.5, (10) Purified water residue (Production method) (1) to (6) ) Oil phase components were mixed, dissolved and heated to 75 ° C. On the other hand, the aqueous phase components (7), (8), and (10) were mixed, dissolved, and heated to 75 ° C. Subsequently, the oil phase component was added to the water phase component and pre-emulsified, and then uniformly emulsified with a homomixer, then cooled to 40 ° C., and (9) was added to obtain a hand cream.

<Prescription Example 12: Hair Lotion>
(1) Polyoxyethylene hydrogenated castor oil (50E.O.) 0.2, (2) Amorseco extract (extract according to Production Example 1) 5.0, (3) Fragrance 0.1, (4) Ethanol 50.0, (5) Panthenol 0.5, (6) 1,3-butylene glycol 5.0, (7) Residue of purified water (Production method) After dissolving (2) and (3) in (1), add (4) and dissolve uniformly. To this was added a homogenized component of (5) to (7) and stirred until uniform to obtain a hair lotion.

<Prescription Example 13: Massage Gel>
(1) Dipropylene glycol 7.0, (2) Glycerin 8.0, (3) Polyoxyethylene (15E.O.) oleyl ether 0.5, (4) 1% by weight carboxyvinyl polymer aqueous solution 40.0, (5) 1% by weight methylcellulose aqueous solution 20.0, (6) Amorseco extract (extract according to Production Example 1) 0.3, (7) 10% by mass potassium hydroxide aqueous solution 1.0, (8) Purified water residue (Production method) (1), (2), (3) , (6) is uniformly dissolved, and (4) and (5) are added to make it uniform. Then, after (8) was added and homogenized, (7) was added and thickened to obtain a massage gel.

  Cell activators, collagen production promoters, whitening agents, antioxidants and fat accumulation inhibitors of the present invention are skin external preparations such as skin cosmetics, cosmetics for hair or cleaning agents, foods and drinks, pharmaceuticals, and quasi drugs. It is useful for blending into products. In addition, since the Amor Seco extract according to the present invention is a naturally derived component, it is easily considered to have high safety, and the significance as a material for external preparations for skin and foods and drinks is also great. It is useful as a new topical skin preparation and food and drink.

Claims (3)

A cell activator comprising Amorseco extract as an active ingredient. Collagen production promoter containing Amorseco extract as an active ingredient. Antioxidant as an active ingredient Amoruseko extract.