JP5071272B2 - Liquid channel device - Google Patents

Description

  The present invention relates to a flat liquid channel device that is suitably used for detecting and analyzing antigens in blood, for example.

In recent years, detection and analysis of trace components in a liquid sample are frequently performed in the medical field, the environmental field, and the like. In this case, for example, in the medical field, a liquid flow called a microchip having a channel formed on a substrate is used. Road equipment is often used.
For example, in Patent Document 1, an antibody-containing reagent and blood are mixed and reacted in a liquid channel formed in a microchip, and then the whole microchip is provided to a detection device to detect an antigen-antibody reaction. The technology to do is described. Further, for example, in Patent Document 2, a plurality of flow paths are formed in the radial direction of a rotatable disk, antibodies are fixed in advance in a part of the flow paths, and then body fluid is circulated through the flow paths. A disc-shaped liquid channel device is disclosed in which an antigen in a body fluid is captured by an antibody by an antigen-antibody reaction.
JP 2007-139500 A JP 05-005741 A

  However, in such a conventional liquid channel device, the liquid channel cannot be closed or opened, which may cause inconvenience in detection and analysis of the target component.

  An object of the present invention is to provide a liquid flow path device that can easily change a liquid flow path from a closed state to an open state at a low cost.

In the liquid channel device of the present invention, a liquid channel through which a liquid composed of at least one of a sample and a reagent flows is formed on at least one surface of the substrate, and the channel forming surface on which the liquid channel of the substrate is formed Is a liquid flow path device in which a cover plate is laminated, and has an opening means for bringing a part of the liquid flow path from a closed state to an open state, and the cover plate forms a first surface of the cover plate. A base material layer, a strong adhesive layer formed inside the first base material layer, a second base material layer formed inside the strong adhesive layer, and formed inside the second base material layer. A weak adhesive layer that adheres to the flow path forming surface, and in the opening means, a first convex portion is formed in the liquid flow channel, and the top of the first convex portion and the weak adhesive layer adhere to each other. In addition, the strong adhesion layer and the second base material layer are separated from each other.
The liquid channel device further includes a closing unit that changes a part of the liquid channel from an open state to a closed state, and the closing unit includes a second convex portion formed in the liquid channel. The top of the part and the weak adhesive layer are separated from each other, and a spacer member is interposed between the strong adhesive layer and the second base material layer, and the spacer member and the strong adhesive layer adhere to each other. Preferably it is.
It is preferable that a measuring tank for measuring a certain amount of liquid is provided in the liquid flow path, the closing means is provided at least upstream of the measuring tank, and the opening means is provided downstream.
It is preferable that the weighing tank is provided with overflow means for overflowing the liquid exceeding the certain amount.

  ADVANTAGE OF THE INVENTION According to this invention, the liquid flow path apparatus which can make a liquid flow path from a closed state to an open state simply can be provided at low cost.

Hereinafter, the present invention will be described in detail.
FIG. 1 is a plan perspective view schematically showing an embodiment of the liquid flow path device of the present invention, FIG. 2 is a plan perspective view in which a part of the liquid flow path device of FIG. 1 is enlarged, and FIG. It is sectional drawing which follows the II 'line.
This liquid flow path device 10A includes a groove-shaped liquid flow path 12 through which a liquid consisting of at least one of a sample and a reagent flows on one side of a flat substrate 11A made of a flat plate, and an end portion or a middle of the liquid flow path 12 A plurality (9 in this example) of liquid reservoirs (14a to 14i) in which liquid is accumulated is formed, and the cover plate 13 is laminated on the flow path forming surface 12a on the side where the liquid flow path 12 of the substrate 11A is formed. It has been done. In this liquid channel device 10A, when the upper end side in FIG. 1 is positioned upward and the lower end side is positioned downward, the end portion on the downstream side from the upstream end portion of the liquid channel 12 is provided. The sample flows in the direction of the arrow F toward the arrow F by gravity, and the sample is mixed with various treatments and reagents in the middle of the sample and used as a measurement solution for various detections and analyses.

That is, at the upstream end of the liquid flow path 12, a sample loading tank 14a in which the loaded sample is stored is provided, and downstream of the sample loading tank 14a, the sample flowing from the sample loading tank 14a is provided. A filtration tank 14b containing a filter (not shown) to be filtered is provided.
A measuring tank 14c for measuring a fixed amount of the filtered sample is provided downstream of the filtering tank 14b. The measuring tank 14c of this example is provided with overflow means comprising an overflow channel 12b and a waste liquid tank 14d provided downstream thereof. Therefore, the sample exceeding a certain amount in the measuring tank 14c overflows and flows through the overflow channel 12b and flows into the waste liquid tank 14d. As a result, the measuring tank 14c can measure a certain amount of sample. .

Downstream of the measuring tank 14c, a first mixing tank 14f is provided in which the sample weighed in the measuring tank 14c and the liquid first reagent previously sealed in the first reagent tank 14e are mixed. Downstream of the first mixing tank 14f is a second mixing in which the intermediate preparation liquid prepared in the first mixing tank 14f and the liquid second reagent sealed in a predetermined amount in the second reagent tank 14g are mixed. A tank 14h is provided.
A measurement tank 14i is provided downstream of the second mixing tank 14h (the end on the downstream side of the liquid flow path 12), and the measurement liquid prepared in the second mixing tank 14h is stored here, and is not illustrated. Detection and analysis means detect and analyze various components.
Each liquid tank is provided with a communication hole (not shown) that communicates with the atmosphere.

Further, as shown in FIG. 3, the lid plate 13 of the liquid channel device 10A includes a first base material layer 13a constituting the surface of the lid plate 13 and a strong member formed on the inner side of the first base material layer 13a. An adhesive layer 13b, a second base material layer 13c formed inside the strong adhesive layer 13b, and a weak adhesive layer 13d formed inside the second base material layer 13c and sticking to the flow path forming surface 12a are provided. Configured.
The first base material layer 13a bends when a load in the vertical direction (a direction perpendicular to the first base material layer 13a) is applied from the surface side, and then returns to the original when the load is removed. It is made of a material having a restoring force to return to On the other hand, the second base material layer 13c is made of a material that is easily bent by the same load and does not recover even when the load is removed, that is, easily plastically deformed. Moreover, the adhesive force of the strong adhesion layer 13b is formed larger than the weak adhesion layer 13d.

Further, the liquid flow path device 10A includes opening means S1 to S7 for bringing a part of the liquid flow path 12 from the closed state to the open state, and a closing means T1 for changing the open state to the closed state.
In this example, the opening means S1 to S7 include the first mixing tank 14a and the filtering tank 14b, the filtering tank 14b and the measuring tank 14c, the measuring tank 14c and the first mixing tank 14f, Between the tank 14f and the second mixing tank 14h, between the first reagent tank 14e and the first mixing tank 14f, between the second reagent tank 14g and the second mixing tank 14h, and between the second mixing tank 14h and the measuring tank One is provided in each liquid flow path 12 between 14i.
On the other hand, the closing means T1 is provided downstream of the opening means S2 in the liquid channel 12 between the filtration tank 14b and the measuring tank 14c.

And in each opening means S1-S7, if S1 and S2 of FIG. 3 are illustrated and demonstrated, the 1st convex part 15 will be formed in the liquid flow path 12, and the top part 15a of this 1st convex part 15 and weak adhesion The layer 13d is adhered, and the strong adhesion layer 13b and the second base material layer 13c are separated from each other.
Therefore, the liquid flow path 12 in each of the opening means S1 to S7 is closed by the first convex portion 15 and the weak adhesive layer 13d adhered to the top portion 15a, and is normally closed. However, as shown in FIG. 4 taking the opening means S1 as an example, the first base material layer 13a in the opening means S1 is pressed from the surface side as indicated by the arrow A, and is perpendicular to the first base material layer 13a. When a load in the direction is applied, as shown in FIG. 4A, the first base material layer 13a bends, and the strong adhesive layer 13b inside the first base material layer 13a becomes the second base material layer 13c. Stick. Then, when the load is removed thereafter, as shown in FIG. 4B, the first base material layer 13a is restored to its original state by its restoring force, and at that time, the first base material layer 13a is adhered to the inside of the first base material layer 13a. The strong adhesion layer 13b, the second substrate layer 13c that adheres to the strong adhesion layer 13b and can be easily plastically deformed, and the weak adhesion layer 13d that adheres to the inside of the second substrate layer 13c are also the first substrate layer. Follows the recovery of 13a and lifts. As a result, the top portion 15a of the first convex portion 15 and the weak adhesive layer 13d are newly separated from each other, and the liquid can flow therethrough.
In this way, in the opening means S1 to S7, after pressing the cover plate 13 from the surface side and applying a load in the vertical direction, by the pressing operation to remove this load, the first convex portion 15 that was originally adhered is removed. The top portion 15a and the weak adhesive layer 13d are separated from each other, and as a result, the liquid flow path 12 in this portion is changed from the closed state to the open state.

On the other hand, in the closing means T1, as shown in FIG. 3, a second convex portion 16 is formed in the liquid flow path 12, and the top portion 16a of the second convex portion 16 and the weak adhesive layer 13d are separated from each other, and A spacer member 17 is interposed between the strong adhesion layer 13b and the second base material layer 13c, and the spacer member 17 and the strong adhesion layer 13b are adhered.
Therefore, in the liquid flow path 12 in the closing means T, the top part 16a of the second convex part 16 and the weak adhesive layer 13d are separated from each other to maintain the flow path, and are normally open. However, as shown to Fig.5 (a), the 1st base material layer 13a in the closure means T1 was pressed from the surface side as shown by arrow B, and the load of the perpendicular direction was added to the 1st base material layer 13a. In this case, the first base material layer 13 a bends, and as a result, the innermost weak adhesive layer 13 d of the cover plate 13 adheres to the top 16 a of the second convex portion 16. Then, when the load is removed thereafter, as shown in FIG. 5B, the first base material layer 13a is restored to its original state by its restoring force, and at that time, the first base material layer 13a is adhered to the inside of the first base material layer 13a. The strong adhesive layer 13b and the spacer member 17 adhered to the strong adhesive layer 13b are lifted following the restoration of the first base material layer 13a. On the other hand, the spacer member 17 is not adhered between the second base material layer 13c and the second base material layer 13c can be easily plastically deformed. The material layer 13c and the weak adhesive layer 13d do not follow the restoration of the first base material layer 13a. As a result, the top portion 16a of the second convex portion 16 and the weak adhesive layer 13d are in an adhesive state, and the liquid channel 12 is closed, so that the liquid cannot flow therethrough.
As described above, in the closing means T1, the top plate 16a of the second convex portion 16 that was originally separated by pressing the cover plate 13 from the surface side and applying a load in the vertical direction and then removing the load. And the weak adhesive layer 13d are adhered and closed, and as a result, the liquid flow path 12 in this portion is changed from the open state to the closed state.

As a specific method for preparing the measurement liquid using the liquid channel device 10A, first, the liquid channel device 10A is set up so that the sample introduction tank 14a side is located above and the measurement tank 14i side is located below. Thus, the liquid easily flows from the upstream side to the downstream side by gravity.
Next, the sample is sampled in a syringe or the like, and the needle of this syringe is pierced into the cover plate 13 corresponding to the sample loading tank 14a to inject the sample into the sample loading tank 14a. Thereafter, the opening means S1 provided between the sample charging tank 14a and the filtration tank 14b is pressed as described above, that is, the first base material layer 13a is pressed from the surface side to add a load, and then removed. The liquid channel 12 in this portion is opened, and the sample is introduced to the filtration tank 14b by gravity.
At this time, the pressing operation may be performed manually by the operator manually pressing the first base material layer 13a from the surface side with a finger, or the pressing position is pre-programmed as XY coordinates, You may make it push a predetermined position.

Next, after the filtration treatment is performed in the filtration tank 14b, the opening means S2 provided between the filtration tank 14b and the measuring tank 14c is operated by pressing operation, and the liquid flow path 12 of this portion is opened, The sample is introduced into the weighing tank 14c by gravity.
Then, after confirming that the introduced liquid has started to overflow in the measuring tank 14c, the closing means T1 provided between the filtration tank 14b and the measuring tank 14c is operated by a pressing operation, and the liquid in this portion The flow path 12 is closed. In this way, after stopping the further flow of the liquid from the upstream side into the measuring tank 14c, the opening means S3 provided downstream of the measuring tank 14c is operated, and the sample measured in the measuring tank 14c. Is introduced into the first mixing tank 14f.

In this way, while the sample after weighing is introduced into the first mixing tank 14f, the opening means S4 between the first reagent tank 14e and the first mixing tank 14f is operated by a pressing operation so that the first reagent is put into the first mixing tank 14f. The sample is introduced into 14f, and the sample and the first reagent are mixed in the first mixing tank 14f to prepare an intermediate preparation solution.
Next, while the opening means S5 between the first mixing tank 14f and the second mixing tank 14h is operated by a pressing operation, the intermediate preparation liquid prepared in the first mixing tank 14f is introduced into the second mixing tank 14h. Then, the opening means S6 between the second reagent tank 14g and the second mixing tank 14h is operated by a pressing operation to introduce the second reagent into the second mixing tank 14h, and the intermediate preparation solution and the second reagent are secondly added. Mix in the mixing tank 14h to prepare a measurement solution.
Next, the opening means S7 between the second mixing tank 14h and the measuring tank 14i is operated, and the measuring solution prepared in the second mixing tank 14h is introduced into the measuring tank 14i.
And after introduce | transducing a measurement liquid into the measurement tank 14i, it uses for a detection analysis means with this liquid flow path apparatus 10A, and a target component is detected and measured.

According to such a liquid circulation device 10 </ b> A, the liquid flow passage 12 has the opening means S <b> 1 to S <b> 7 for changing the liquid flow path 12 from the closed state to the open state and the closing means T <b> 1 for changing the open state to the closed state. As a result, highly accurate detection and analysis can be performed in a short time.
For example, in this example, closing means T1 is provided upstream of the weighing tank 14c, and opening means S3 is provided downstream. Therefore, the sample can be accurately weighed in a short time in the weighing tank 14c and introduced into the first mixing tank 14f. Here, if the opening means S3 is not provided downstream of the measuring tank 14c, and the liquid flow path 12 in this portion is always open, the sample continuously from the measuring tank 14c even during measurement. The sample cannot be collected in a certain amount, and measurement itself is impossible. In addition, when the closing means T1 is not provided upstream of the measuring tank 14c, after the entire amount of the sample that has passed through the filtration tank 14b has completely flowed into the measuring tank 14c, the measuring tank 14c and the first mixing tank 14f. It is necessary to introduce the weighed sample into the first mixing tank 14f by actuating the opening means S3 between the two. In this case, if the sample is a liquid having viscosity such as blood, it takes time until the entire amount of the sample passing through the filtration tank 14b completely flows into the measuring tank 14c, and it is difficult to measure in a short time. It becomes. In this respect, if the closing means T1 is provided between the upstream of the measuring tank 14c as in this example, even if the entire amount of the sample passing through the filtration tank 14b does not completely flow into the measuring tank 14c, the measuring tank When the sample starts to overflow in 14c, the closing means T1 can be operated to stop further inflow of the sample into the measuring tank 14c, and accurate measurement can be performed in a short time.

  In this example, an opening means S5 is provided between the first mixing tank 14f and the second mixing tank 14h, and an opening means S7 is provided between the second mixing tank 14h and the measurement tank 14i. Therefore, in the first mixing tank 14f and the second mixing tank 14h, after the target mixing and reaction have sufficiently progressed, the opening means S5 and S7 are opened, and the intermediate preparation liquid and the measurement liquid are respectively supplied to the second mixing tank. 14h and the measuring tank 14i. Therefore, it is possible to prevent a decrease in detection and analysis accuracy due to insufficient mixing and reaction.

  Furthermore, in this example, since the opening means S4 and S6 are provided between the first reagent tank 14e and the first mixing tank 14f and between the second reagent tank 14g and the second mixing tank 14h, it is desirable. These are opened at the point of time, and the first reagent and the second reagent previously sealed in the first reagent tank 14e and the second reagent tank 14g, respectively, are caused to flow into the first mixing tank 14f and the second mixing tank 14h. Can do. If the opening means S4 and S6 are not provided, the first reagent and the second reagent may start to flow downstream when the liquid channel device 10A is stored.

  Further, the opening means S1 to S7 and the closing means T1 of the liquid channel device 10A of this example are a combination of the first convex portion 15 and the second convex portion 16 formed in the liquid channel 12 and the lid plate 13. Since it is a structure, it is not necessary to prepare another member for opening and closing the liquid flow path 12, and it is low-cost and the structure is also simple. In addition, the opening and closing operations can be performed only by a simple pressing operation, and the operability is excellent.

  FIG. 6 shows another embodiment of the liquid flow path device of the present invention. This liquid flow path device 10B has a liquid flow path described above on one side of a substrate 11B made of a fan-shaped flat plate. Similarly to the apparatus 10A, a groove-like liquid channel 12 through which a liquid consisting of at least one of a sample and a reagent flows, and a plurality of liquid tanks (14a, 14e to 14h) in which the liquid is accumulated at the end or in the middle of the liquid channel 12 14J, 14k, 14m), and the cover plate 13 is laminated on the flow path forming surface 12a of the substrate 11 on the side where the liquid flow path 12 is formed. In this liquid flow path device 10B, when rotated about the upper end side in FIG. 6, the direction from the upstream end to the downstream end of the liquid flow path 12 in the direction of arrow F ′ The sample is circulated by centrifugal force, and the sample is mixed with various treatments and reagents in the middle of the sample to obtain a measurement solution.

That is, the upstream end of the liquid channel 12 is provided with a sample introduction tank 14a in which the introduced sample is stored, and the first reagent and the first reagent from the first reagent tank 14e are provided downstream of the sample introduction tank 14a. There is provided a first mixing tank 14f in which the second reagent from the second reagent tank 14g and the sample from the sample charging tank 14a are mixed to prepare an intermediate preparation liquid.
Downstream of the first mixing tank 14f is a second mixing in which the third reagent from the third reagent tank 14j, the fourth reagent from the fourth reagent tank 14k, and the intermediate preparation liquid from the first mixing tank 14f are mixed. A tank 14h is provided.
In this example, the second mixing tank 14h also acts as a measurement tank, and various components are detected and analyzed by a detection analysis unit (not shown) with respect to the measurement liquid prepared in the second mixing tank 14h. It has become.
In this example, a disposal tank 14m is formed for storing the measurement liquid after being measured in the second mixing tank 14h.
Each liquid tank is provided with a communication hole (not shown) that communicates with the atmosphere.

 Also in this liquid channel device 10B, the lid plate 13 has the same configuration as shown in FIG. 3 as in the case of the above-described liquid channel device 10A, that is, the first base material layer 13a constituting the surface of the lid plate 13 and A strong adhesive layer 13b formed inside the first base material layer 13a, a second base material layer 13c formed inside the strong adhesive layer 13b, and formed inside the second base material layer 13c. It has a configuration having a weak adhesive layer 13d that adheres to the path forming surface.

Further, between the sample charging tank 14a and the first mixing tank 14f, between the first sample tank 14e and the first mixing layer 14f, between the second sample tank 14g and the first mixing tank 14f, and between the first mixing tank 14f and the first mixing tank 14f. 14f and the second mixing tank 14h, the third sample tank 14j and the second mixing layer 14h, the fourth sample tank 14k and the second mixing tank 14h, the second mixing tank 14h and the disposal tank 14m. Are provided with one opening means S8 to S14 for bringing the liquid flow path 12 from the closed state to the open state.
On the other hand, in the liquid flow path 12 between the sample introduction tank 14a and the first mixing tank 14f, a closing means T2 is provided on the downstream side of the opening means S8.

  As in the case of the liquid channel device 10 </ b> A, in each of the opening means S <b> 8 to S <b> 14, as illustrated in FIG. 3, the first convex portion 15 is formed in the liquid channel 12. The top portion 15a and the weak adhesive layer 13d are adhered to each other, and the strong adhesive layer 13b and the second base material layer 13c are separated from each other. On the other hand, in the closing means T2, the second convex portion 16 is formed in the liquid channel 12, the top portion 16a of the second convex portion 16 and the weak adhesive layer 13d are separated from each other, and the strong adhesive layer 13b and the second base are formed. A spacer member 17 is interposed between the material layer 13c and the spacer member 17 and the strong adhesion layer 13b are adhered.

When preparing a measurement liquid using the liquid channel device 10B, first, the liquid channel device 10B is positioned on the rotation center side on the sample loading tank 14a side, and the measurement tank 14i side is positioned on the outer peripheral side of the rotation. Set to centrifuge.
Next, the sample is sampled in a syringe or the like, and the needle of this syringe is pierced into the cover plate 13 corresponding to the sample loading tank 14a to inject the sample into the sample loading tank 14a. Thereafter, by operating the centrifugal device, a centrifugal force acting from the rotation center side to the outer peripheral side is generated, and by this centrifugal force, the liquid starts to flow from the upstream side to the downstream side.
Next, the opening means S8 provided between the sample charging tank 14a and the first mixing tank 14f is operated by the same pressing operation as in the liquid channel device 10A, and the liquid channel 12 in this part is opened. The sample is introduced into the first mixing tank 14f by centrifugal force.

While the sample is introduced into the first mixing tank 14f while the centrifugal force is generated in this way, the opening means S9 between the first reagent tank 14e and the first mixing tank 14f is operated by a pressing operation, and is enclosed in advance. The first reagent which has been introduced is introduced into the first mixing tank 14f, and then the opening means S10 between the second reagent tank 14e and the first mixing tank 14f is operated by a pressing operation, so that the second sealed in advance The reagent is also introduced into the first mixing tank 14f, and the sample, the first reagent, and the second reagent are mixed in the first mixing tank 14f.
At this time, if necessary, before the entire amount of the sample completely flows into the first mixing tank 14f, the closing means T2 is operated, whereby the sample is excessively flowed into the first mixing tank 14. Can also be stopped.

Next, the opening means S11 between the first mixing tank 14f and the second mixing tank 14h is operated by a pressing operation, and the intermediate preparation liquid prepared in the first mixing tank 14f is introduced into the second mixing tank 14h. Then, the opening means S12 between the third reagent tank 14g and the second mixing tank 14h and the opening means S13 between the third reagent tank 14g and the second mixing tank 14h are actuated by pressing operation in advance. The sealed third reagent and fourth reagent are also introduced into the second mixing tank 14h, and the intermediate preparation liquid, the third reagent, and the fourth reagent are mixed in the second mixing tank 14h.
Then, the entire liquid flow path device 10B is provided for detection analysis means, and the target component is detected and analyzed for the measurement liquid prepared in the second mixing tank.
After the detection and analysis are completed, the opening means S14 can be operated to discard the measurement liquid after measurement into the waste tank 14m.

Such a liquid circulation device 10B also includes the opening means S8 to S14 that bring the liquid flow path 12 from the closed state to the open state and the closing means T2 that turns the liquid flow path 12 from the open state to the closed state. The flow of the liquid can be controlled, and as a result, detection and analysis can be performed with good accuracy in a short time.
Further, the opening means S8 to S14 and the closing means T2 are low in cost and simple in configuration, and can be operated only by a simple pressing operation.

Also in the case of the liquid channel device 10B of this example, the pressing operation in the opening means S8 to S14 and the closing means T2 can be performed manually, but by a pressure contact disk that presses the surface of the lid plate 13 of the liquid channel device 10B. When pressed, a plurality of liquid flow path devices 10B can be continuously pressed, which is preferable.
FIG. 7 shows a plurality of (six in this example) fan-shaped (center angle α = 60 °) liquid channel device 10B arranged in a circular shape on a base plate 20 of the centrifugal device. The method of pressing with the press contact disk 21 is shown. The pressure contact disk 21 in this example is provided on an arm 23 extending laterally from a rotation shaft 22 of a centrifugal device that rotates the liquid flow path device 10B, and rotates in the length direction of the arm 23 while rotating around the arm 23. Along the (radial direction of rotation of the liquid flow path device), the liquid flow path device 10B moves from the rotation center side toward the outer peripheral side. Therefore, by moving the pressure contact disk 21 in this manner while rotating the liquid flow path device 10B by the centrifugal device, the pressure contact disk 21 moves on the liquid flow path device 10B arranged in a circle from the rotation center side to the outer peripheral side. Scanning relatively spirally toward each other, the opening means S8 to S14 and the closing means T2 provided in the liquid channel device 10B can be sequentially pressed.

  In the above description, as a method of injecting a sample into the sample introduction tank 14a of the liquid flow path apparatuses 10A and 10B, a method of piercing the needle of the syringe into the cover plate 13 is exemplified. Alternatively, a sample injection hole may be formed, and the sample may be injected therefrom. In that case, the sample injection hole may be covered with a protective tape and injected by piercing the syringe with the protective tape, or the protective tape may be peeled off and the syringe inserted into the sample injection hole for injection.

In the liquid flow path apparatuses 10A and 10B described above, the substrates 11A and 11B on which the liquid flow path 12 and the liquid tank are formed include, for example, styrene resin, acrylic resin, polycarbonate resin, vinyl chloride resin, PEN resin, and polyester resin. A resin plate such as an epoxy resin, a phenol resin, an ABS resin, a polypropylene resin, or a fiber reinforced plastic, or a glass plate can be used. Among these, a glass plate, a styrene resin, an acrylic resin, a polycarbonate resin, and vinyl chloride are transparent in that the state of the liquid flowing through the liquid flow path 12 can be visually observed from the side of the substrates 11A and 11B. Resins, PEN resins, and polyester resins are preferred. In addition, a resin plate is preferable in that it is less likely to break than a glass plate and has excellent handleability.
The thickness of the substrates 11A and 11B is not particularly limited, and may be determined according to the depth of the liquid channel 12 to be formed, but is usually 0.5 to 7 mm.

The liquid flow path 12 and the liquid tank are formed in a groove shape on one surface of the substrates 11A and 11B by techniques such as photolithography, injection molding, blow molding, bonding formation, dissolution formation, cutting formation, and machining.
There is no particular limitation on the cross-sectional shape of the liquid flow path 12 (the cross-section in the direction perpendicular to the flow), and examples thereof include a semicircular shape, a quadrangular shape, and an inverted triangular shape. The width and depth of the liquid channel 12 are not particularly limited and may be determined according to the required flow rate of the liquid. However, if the width and depth are in the range of 10 to 5000 μm, the small channel It is preferable in that a liquid can be flowed by resistance and a small amount of liquid can be circulated.
The liquid flow path 12 is preferably subjected to a surface treatment according to the type of liquid in order to facilitate the flow of the liquid. Examples of such surface treatment include coating application treatment, plasma treatment, flame treatment, chemical treatment, physiological activity treatment, and antibody treatment. Furthermore, the liquid flow path 12 may be provided with a baffle plate, a stirring plate, a protrusion, or formed with a water diverting shape, if necessary, so that the flowing liquid is in a uniform mixed state. .
Each liquid tank is not particularly limited in shape, and may be appropriately formed according to the volume required for each liquid tank.

  The first base material layer 13a constituting the surface of the cover plate 13 has a restoring force to bend and then return when a vertical load is applied from the surface side. If it is a base material having such characteristics, that is, flexibility and restoring force, it can be used as the first base material layer 13a, and its material and thickness are not particularly limited, but polyethylene terephthalate (PET), A film having a thickness of 50 to 500 μm made of polyethylene naphthalate (PEN), polycarbonate (PC), polyimide, or the like is preferable because it has flexibility and resilience appropriate for use as the first base material layer 13a. .

  On the other hand, the 2nd base material layer 13c should just be easily bent by the load of a perpendicular direction, and the thing which does not restore | restore is still more preferable. If it is the base material which has such a characteristic, it can be used as the 2nd base material layer 13c, Although there is no restriction | limiting in particular in the material and thickness, Metal foil, such as aluminum foil and copper foil, paper, PET, PEN, A film having a thickness of 5 to 50 μm made of a resin such as PC or polyimide is preferable for use as the second base material layer 13c. When using paper, waterproof paper is preferable, and when using metal foil, rust-proof metal foil is preferable.

The strong adhesive layer 13b and the weak adhesive layer 13d can be appropriately selected from conventionally known adhesives according to the material of the first base material layer 13a and the second base material layer 13c. The adhesive force (adhesive strength) of the adhesive forming the strong adhesive layer 13b needs to be stronger than the adhesive force of the adhesive forming the weak adhesive layer 13d. When the pressure-sensitive adhesive of the pressure-sensitive adhesive forming the strong pressure-sensitive adhesive layer 13b is equal to or lower than the pressure-sensitive adhesive of the pressure-sensitive adhesive forming the weak pressure-sensitive adhesive layer 13d, even when a pressing operation is performed in the opening means S1 to S14, 15a and the weak adhesion layer 13d cannot be separated, and the liquid flow path 12 cannot be opened. It is preferable that the adhesive strength of the adhesive forming the strong adhesive layer 13b is 0.1 N / cm or more larger than the adhesive strength of the adhesive forming the weak adhesive layer 13d. Furthermore, it is preferable that it is large in the range of 0.1-30 N / cm. When the adhesive force of the adhesive forming the strong adhesive layer 13d is 0.1 N / cm or more larger than the adhesive force of the adhesive forming the weak adhesive layer 13d, the opening means S1 to S14 can be operated reliably. On the other hand, it is difficult to configure these adhesive layers so that the difference in adhesive strength exceeds 30 N / cm.
Moreover, it is preferable that the adhesive force of the strong adhesive layer 13b is in the range of 1 to 30 N / cm and the adhesive force of the weak adhesive layer 13d is in the range of 0.05 to 5 N / cm.

Examples of the adhesive used for the strong adhesive layer 13b and the weak adhesive layer 13d include acrylic, rubber-based, polyurethane-based, polyester-based, and silicon-based adhesives. Among these, for example, the strong adhesive layer 13b may be made of acrylic or rubber, and may further include a nonwoven fabric, polyester fiber, or the like as a core material. The weak adhesive layer 13d is preferably made of acrylic or silicon. In order to make the difference in adhesive strength between the strong adhesive layer 13b and the weak adhesive layer 13d within the above-mentioned preferable range, the glass transition temperature of the resin constituting each adhesive is appropriately adjusted, or the tackifier is added to the adhesive. And a method of adding additives such as a curing agent and a core material and adjusting the amount of the additive.
Moreover, although there is no restriction | limiting in the thickness of a strong adhesion layer and a weak adhesion layer, Usually, it is 10-1000 micrometers.

  Here, “adhesive strength” refers to the adhesive strength that peels 180 degrees from a stainless steel plate of JIS Z 0237.

  As the spacer member 17, paper, etc. can be used in addition to resins such as PET, PEN, PC, acrylic resin, epoxy resin, phenol resin, and polyurethane resin. Although there is no restriction | limiting in particular in the thickness of the spacer member 17, If it is set as the range of 50-2000 micrometers, before the opening means S1-S14 act | operates, the strong adhesion layer 13b and the 2nd base material layer 13c will be spaced apart reliably. On the other hand, at the time of operation, the strong adhesion layer 13b and the second base material layer 13c can be reliably adhered.

In the above description, the liquid flow path devices 10A and 10B in which the liquid flow path 12 is formed only on one side of the substrates 11A and 11B are illustrated, but the liquid flow path 12 is formed on both sides of the substrates 11A and 11B. May be.
In addition, the communication holes provided in each liquid tank are preferably sealed before use of the liquid channel devices 11A and 11B and opened during use from the viewpoint of preventing contamination. Therefore, an opening means having the same configuration as the opening means S1 to 14 provided in the liquid channel 12 may be provided before the communication hole.
In the above description, in order to circulate the liquid, the liquid channel device 10A uses gravity and the liquid channel device 10B uses centrifugal force. However, the method is not limited thereto. For example, a part of the liquid channel 12 is pressurized, a part of the liquid channel 12 is heated to expand the air in the liquid channel 12, or an oxygen absorbent (oxidation agent) is added to a part of the liquid channel 12. It is also possible to employ a method in which a liquid is moved and circulated, for example, by enclosing an iron powder or the like that is easy to do and absorbing oxygen in the liquid channel 12 to reduce the pressure in the liquid channel 12. Alternatively, the measuring tank 14 may be pressurized, heated, or depressurized instead of a part of the liquid channel 12, or depending on the case, both the liquid channel 12 and the measuring tank 14 may be pressurized, heated, or depressurized. May be.

There are no particular limitations on the sample and reagent through which the liquid flow path devices 10A and 10B are circulated, and a sample and a reagent that have been conventionally employed in the medical field, the environmental field, and the like can be used in appropriate combination. For example, in the medical field, samples (such as blood (whole blood), plasma, serum, buffy coat, urine, feces, saliva, sputum, etc.), viruses, bacteria, fungi, yeast, animal and plant cells, etc. Is mentioned. In addition, DNA or RNA isolated from these may be used, or a sample obtained by subjecting these to any pretreatment or dilution may be used. As a reagent, when an antigen present in a sample is analyzed, a reagent containing an antibody against the antigen is preferable.
Moreover, conventionally known optical means, electrical means, and the like can be appropriately employed as detection / analysis means for the measurement liquid prepared by the liquid flow path apparatuses 10A and 10B.

It is a schematic plane perspective view showing an embodiment of the liquid channel device of the present invention. FIG. 2 is an enlarged plan perspective view of a part of the liquid channel device in FIG. 1. It is sectional drawing which follows the I-I 'line of FIG. It is explanatory drawing explaining a mode that an opening means act | operates. It is explanatory drawing explaining a mode that a closing means act | operates. It is a schematic plane perspective view which shows another example of embodiment of the liquid flow-path apparatus of this invention. It is a schematic explanatory drawing explaining an example of the usage method of the liquid flow-path apparatus of FIG.

Explanation of symbols

10A, 10B Liquid channel device 11A, 11B Substrate 12 Liquid channel 12a Channel forming surface 13 Cover plate 13a First substrate layer 13b Strong adhesion layer 13c Second substrate layer 13d Weak adhesion layer 14c Weighing tank 15 First convex Part 15a Top part 16 of the first convex part Second convex part 16a Top part 17 of the second convex part Spacer members S1 to S14 Opening means T1 to T2 Closing means

Claims (4)

A liquid channel in which a liquid channel including at least one of a sample and a reagent circulates is formed on at least one surface of the substrate, and a lid plate is laminated on the channel forming surface of the substrate on which the liquid channel is formed A device,
An opening means for opening a part of the liquid flow path from a closed state to an open state;
The lid plate includes a first base layer constituting the surface of the lid plate, a strong adhesive layer formed inside the first base layer, and a second base formed inside the strong adhesive layer. A material layer and a weak adhesive layer formed on the inner side of the second base material layer and sticking to the flow path forming surface;
In the opening means, a first convex portion is formed in the liquid flow path, a top portion of the first convex portion and the weak adhesive layer are adhered, and the strong adhesive layer and the second base material layer are bonded to each other. A liquid flow path device characterized by being spaced apart. A closing means for closing a part of the liquid channel from an open state to a closed state;
In the closing means, a second convex portion is formed in the liquid flow path, a top portion of the second convex portion and the weak adhesive layer are separated from each other, and the strong adhesive layer and the second base material layer are separated from each other. The liquid channel device according to claim 1, wherein a spacer member is interposed between the spacer member and the strongly adhesive layer.   The liquid channel is provided with a measuring tank for measuring a certain amount of liquid, the closing means is provided at least upstream of the measuring tank, and the opening means is provided downstream. The liquid channel device according to claim 2.   The liquid channel device according to claim 3, wherein the weighing tank is provided with overflow means for overflowing the liquid exceeding the predetermined amount.

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