JP5009533B2 - Reagent container - Google Patents

Description

  The present invention relates to a reagent container.

In recent years, μ-Total Analysis System technology and Lab-on-Chip technology for performing chemical reactions, DNA reactions, protein reactions, etc. on the chip have been researched and realized, and so far large-scale experimental devices and a large amount of reagents are required. It is now possible to perform reaction experiments with a small amount of reagent on a chip of several millimeters or less.
Examples of the DNA reaction include DNA amplification reaction by enzymatic reaction, a method of detecting a sample DNA sequence by a hybridization method using a probe DNA having a known sequence, and SNP (single nucleotide polymorphism) in DNA sequencing. ) Detection method.
As a method for detecting SNP, a method using an invader method or a Tuckman PCR method in a typing process is known (see Patent Document 1).

In general, a detection reaction using DNA is performed by collecting and extracting blood or the like. However, in order to use a small amount of sample such as blood to be collected, a DNA amplification reaction by an enzymatic reaction is used as a method for preparing a sample DNA. There are many cases.
As a method for increasing a small amount of DNA contained in a document, a main method is known, and a PCR amplification reaction is known as a typical method. This method consists of three steps consisting of a separation step of double-stranded DNA in a sample (denaturation into single strands), an annealing step (binding single-stranded DNA and primers), and an extension step (synthesize DNA from primers). Is one cycle, and this cycle is repeated to increase the DNA in the sample. Since the separation step is performed at about 95 ° C., the annealing step is performed at 50 to 60 ° C., and the extension step is performed at 60 to 80 ° C., the PCR amplification reaction needs to repeat this thermal cycle. The time required for one cycle is about several minutes at most, but it takes some time to amplify a necessary amount of DNA by repeating this cycle.

The invader method, which is one of the SNP detection methods, is partially self-hybridized to become double-stranded, and has a fluorescent label (reporter) and a quencher that deactivates fluorescence (fret probe). In this method, two types of unlabeled oligonucleotides (allele probe, invader probe) and restriction enzyme (chrybase) are allowed to act, and SNP is detected by fluorescence detection.
In this invader method, first, a part of the allele probe is hybridized to one side of the base sequence of the DNA to be examined centering on the SNP. The invader probe is then hybridized to the other side of the test object. At this time, the test object, the allele probe, and the invader probe are in a state where they are overlapped at the SNP site. A restriction enzyme (chrybase) is allowed to act on the triple overlapped portion to cleave and release the portion (flap) that has not hybridized with the DNA to be examined by the allele probe.

  Next, the released flap is hybridized with the fret probe. As a result, the flap partially overlaps with the self-hybridized portion of the fret, and the fret probe and the flap are overlapped in triplicate. The fret probe has a fluorescent label (reporter) and a quencher that inactivates fluorescence. When a restriction enzyme (cribase) is allowed to act on this triplet, the fluorescent label (reporter) is released and the quencher is released. Since it is separated from the char, it emits fluorescence. In this case, ultraviolet light is generally used as the excitation source.

When these reactions are performed using a chip, or when the sequence of DNA is determined, a method is known in which probe DNA is immobilized on a slide glass and a hybridization reaction is performed thereon.
It is also known that a minute hole or depression called a well provided on a chip is formed and used as a reaction field. The well has been formed by etching a semiconductor or glass or laminating a plate with holes.
In the case of using wells, it is not necessary to fix the reagent on the substrate, and it can be applied to a PCR reaction or the like.
In addition, these wells can be used as a mixing place with other reagents such as a buffer solution after a very small amount of reagent is filled in a predetermined position.
As a well-type substrate, for example, a detection substrate having a large number of wells provided on the substrate surface is disclosed (see Patent Documents 2, 3, and 4).
An apparatus for PCR reaction is also known which has a flow path inside and has openings at both ends (see Patent Document 5).
These all supply reagents to the cavity in the well, but the amount of reagents to be filled such as enzymes and nucleic acids is extremely small. Here, when the reagent is injected into the well or the reagent in the well is dispensed, the reagent is aspirated and discharged by an injection needle or a dropper.
Japanese Patent Laid-Open No. 2002-300894 International Publication No. 2003/031972 Pamphlet JP 09-99932 A JP 2003-70456 A Japanese Patent No. 2759071

  However, there is a problem that, when trying to suck and discharge with an injection needle or the like, the reagent in the well is scattered outside the well. That is, when injecting a reagent into the well, the reagent is discharged into the well from an injection needle or the like. At this time, the reagent may be scattered outside the well. Also, when separating the reagent in the well, an injection needle is inserted into the reagent and the reagent is aspirated. However, when the injection needle is inserted into the reagent or when the reagent is aspirated, the reagent is further aspirated. Then, when the injection needle or the like is pulled out, the reagent may be scattered out of the well. And if a reagent scatters out of a well, there exists a possibility that a reagent may mix in another well.

  The present invention has been made in view of such circumstances, and can easily and appropriately perform the injection of the reagent into the recess or the flow path, the separation of the reagent from the recess or the flow path, and the like. An object of the present invention is to provide a reagent container capable of performing a test quickly and with high accuracy.

In order to solve the above problems, the present invention provides the following means.
The reagent container according to the present invention includes a recess or a channel into which a reagent is injected, a first lid member that covers an opening of the recess or the channel, and the opening through the first lid member. e Bei and second cover members for covering, a clearance is provided between the said first cover member and the second lid member, the recess, and a large-diameter concave portion constituting the opening portion of the recess A small-diameter concave portion provided at the bottom of the large-diameter concave portion and having a smaller diameter than the large-diameter concave portion, the first lid member being provided in the small-diameter concave portion, A second lid member is provided .

In the reagent container according to the present invention, the injection needle, the syringe, or the like is passed through the first lid member and the second lid member, the reagent is injected into the recess or the channel, and is injected into the recess or the channel. Collect the reagent. At this time, the movement of the droplets of the reagent trying to leak out of the recess or the flow path is restricted by the first lid member and the second lid member.
Thereby, it is possible to prevent the reagent from splashing out of the recess or the flow path by the first lid member and the second lid member.
Further, an injection needle or the like is passed through the first lid member and the second lid member through the clearance, the reagent is injected into the recess or the channel, and the reagent injected into the recess or the channel is collected. . At this time, the movement that the droplets of the reagent try to leak out to the outside of the recess or the flow path is first regulated by the first lid member. Even if the reagent leaks out of the first lid member, the reagent enters the clearance, and the movement of the reagent in the clearance that attempts to leak out of the recess or the flow path is the second It is regulated by the lid member.
Thereby, it can prevent reliably that a reagent scatters out of a recessed part or a flow path.
Moreover, a clearance can be reliably provided between the first lid member and the second lid member.

In the reagent container according to the present invention, a convex portion raised upward is provided around the opening of the concave portion, and the second lid member is provided so as to contact the convex portion. It is characterized by.

The reagent container according to the present invention includes a plurality of the concave portions, and the convex portions provided around the openings of the plurality of concave portions are connected to each other at the same height. .

Further, in the reagent container according to the present invention, the reaction portion includes a plurality of through holes formed in the substrate, a groove portion formed over the plurality of through holes on one surface of the substrate, and the substrate of the substrate. It is characterized by comprising a film bonded to one surface and covering the groove .

In the reagent container according to the present invention, the first lid member is a film including a hard aluminum layer and a seal layer .

In the reagent container according to the present invention, the injection needle can be easily passed through the first lid member and the second lid member. Furthermore, it is possible to prevent the peripheral edge of the through-hole from coming into close contact with the outer peripheral surface of the injection needle or the like, and to ensure the gas flow through the gap between the peripheral edge of the through-hole and the outer peripheral surface of the injection needle or the like. it can. Therefore, the reagent can be sucked and discharged easily and quickly from an injection needle or the like.

  According to the present invention, it is possible to prevent the reagent from splashing out of the recess or the channel, so that the injection of the reagent into the recess or the channel, the separation of the reagent from the recess or the channel, etc. The test can be performed easily and appropriately, and the test can be performed quickly and with high accuracy.

Hereinafter, the reagent container according to the first embodiment of the present invention will be described with reference to the drawings. In this embodiment, a reagent container used in the μ-Total Analysis System technology or the Lab-on-Chip technology for performing a chemical reaction, a DNA reaction, a protein reaction or the like on a chip will be described as an example.
In FIG. 1, the code | symbol 1 has shown the container for reagents.
The reagent container 1 includes a substantially rectangular and plate-like substrate 2 whose vertical and horizontal dimensions are set to several millimeters or less.

The material of the substrate 2 may be any material that does not adversely affect the reagent. In addition, when the reagent is reacted on the substrate 2, it is necessary that the reaction system is not adversely affected. Furthermore, when detecting the reaction, when optical detection is performed from below the substrate 2, it is preferable that the transparency is high.
As such a thing, PC (polycarbonate), PP (polypropylene), a cycloolefin type polymer, a fluoropolymer, a silicone resin etc. can be used, for example.
In addition, cycloolefin resin (Zeonor (manufactured by ZEON Corporation)) and methylpentene resin (TPX (manufactured by Mitsui Chemicals))) are used in terms of transparency, heat resistance, chemical resistance and influence on the reaction system. It is preferable to use it.
It is preferable to make the substrate 2 using such a synthetic resin because it is excellent in heat resistance, chemical resistance, moldability, and the like. Further, two or more kinds of resins may be joined and used. In this case, by making the substrate 2 by making use of the characteristics of each resin, it is possible to make various substrates 2 according to the characteristics of the reagent, the sample, and the like, which can be used for each application. For example, it is possible to divide the material between the upper half and the lower half of the substrate 2. Further, the material can be divided for each part such as a reagent storage unit 8 and a reaction unit 6 described later.
Glass may be used as the material for the substrate 2.

In a region on one end side in the length direction of the substrate 2, a well-like reagent storage portion 8 that is depressed in the thickness direction of the substrate 2 is formed. The reagent storage unit 8 is for receiving a reagent after it is injected. That is, the reagent storage unit 8 functions as a recess.
The reagent container 8 can be formed by cutting or molding if the material of the substrate 2 is plastic or synthetic resin, and can be formed by cutting if the material of the substrate 2 is glass. Can do.
Moreover, the number of the reagent storage parts 8 can be suitably set according to the purpose. When a plurality of reagent storage units 8 are provided, the sizes may be different.

In addition, the reagent storage unit 8 can be used as a mixing place in which one reagent is put in advance and another reagent is put and mixed later.
The volume of the reagent storage unit 8 is preferably in the range of 10 to 300 μl. In life science fields such as chemical reactions, especially biochemical reactions and biological reactions that handle DNA, trace amounts of reagents are often used. The DNA collected from blood is usually about several hundred nl to several μl at most. Therefore, the number of reagents, diluents, etc. used in the reaction is at most several hundred μl, and is preferably within the above-mentioned range.

  On the other hand, in the region on the other end side in the length direction of the substrate 2, a well-shaped detection unit 7 that is depressed in the thickness direction of the substrate 2 is formed. A plurality of detection units 7 are formed in the vertical and horizontal directions of the substrate 2. These detection parts 7 are preferably in the shape of a truncated cone having a flat bottom part and an inclined side wall surface from the opening part 7a to the bottom part. If the detection unit 7 has a truncated cone shape, it is advantageous for optical detection from below. This is because, for example, when the fluorescent state of the fluorescent material is detected from below by putting a fluorescent material in the detection unit 7 and irradiating ultraviolet rays from below, the shape of the detection unit 7 is spherical or other complicated shape. This is because the amount of ultraviolet light that is an excitation source of the fluorescent material is refracted and scattered, and the amount of the fluorescent material irradiated is reduced. In addition, the generated fluorescence is also refracted and scattered, which causes a decrease in detected fluorescence intensity, false detection, and the like.

In addition, you may provide the protective film which covers the opening part 7a in the detection part 7. FIG. By providing the protective film, it is possible to prevent contamination by dust, contaminants, and the like.
A film-like thing can be used as a protective film. Examples of such films include polyolefin films such as polyethylene, polypropylene and polymethylpentene, acrylic films such as polymethyl acrylate and polymethyl methacrylate, polystyrene films, polyacetal films, polyamide films, polyacrylonitrile films, polycarbonate films, Examples include olefin-based films, silicon resin-based films, and fluorine-based resin films. Moreover, you may use metal foil, such as aluminum, and the laminated film of metal foil and the above-mentioned resin film. These protective films can be bonded using an adhesive. As the adhesive, a heat-resistant curable adhesive can be used. Further, it may be bonded by heat sealing. When the reagent is stored in the detection unit 7 in advance, it is preferable to use heat sealing because it is not necessary to consider the influence of the adhesive on the reagent. Moreover, since it is necessary to peel off a protective film before using, it is preferable that it is easy to peel.

Moreover, as shown in FIG. 2, you may provide the convex part 25 raised upwards around the opening part 7a. By providing the convex portion 25, the protective film can be easily bonded. In particular, when pasting together by heat sealing, the portion to be heated is not the entire base material 14 (shown in FIG. 7), which will be described later, but only a place where it comes into contact with the convex portion 25. In this case, the thermal influence on the reagent can be reduced.
Further, when the protective film is peeled off, the contact between the protective film and the substrate 2 is not on the whole substrate 2 except the opening 7a but only on the convex portion 25, so that the protective film can be easily peeled off.
Such a convex portion 25 preferably has a width in the range of 0.1 to 3 mm and a height in the range of 0.05 to 2 mm.
Further, the convex portions 25 may be connected at the same height as the convex portions 25. By doing so, when peeling, it can be smoothly peeled without being caught.

Furthermore, one well-like reaction portion 6 that is depressed in the thickness direction is formed in a central region in the length direction of the substrate 2 (region sandwiched between the detection unit 7 and the reagent storage unit 8). .
The reaction part 6 can be formed by cutting or molding if the material of the substrate 2 is plastic or synthetic resin. Moreover, if the material of the board | substrate 2 is glass, it can form by cutting. The opening diameter of the reaction part 6 (the diameter of the opening part 6a) is preferably in the range of 0.1 to 5 mm and the depth is preferably in the range of 0.1 to 5 mm. As described above, in the life science field, a small amount of reagent is often used, and in order to perform the reaction efficiently, it is preferably within the above range. Further, the reaction liquid can be overlaid with a non-volatile liquid having a specific gravity lower than that of the reaction liquid to prevent evaporation. Nonvolatile liquids include mineral oil, silicone oil, vegetable oil, animal oil, liquid paraffin, and the like.
Further, the reaction part 6 may be covered with a film or the like.

  The side section of the reaction part 6 (the longitudinal section in the depth direction of the reaction part 6) is not particularly limited, but the side part constituting the opening 6a is cylindrical and the bottom part is hemispherical, or the whole Is preferably hemispherical. If it is hemispherical, when the reagent is injected, it is possible to prevent scattering of the reagent and mixing of bubbles as much as possible. Moreover, it becomes excellent in the taking-out property at the time of taking out the injected reagent.

Moreover, although the one reaction part 6 was provided, the number of installation can be suitably set according to the objective. For example, when performing 2 or more types of reaction, you may provide a 2nd reaction part.
For example, when the reaction is used for a DNA detection reaction, the second reaction part can be a PCR reaction part. By providing a PCR reaction section, sample preparation and DNA detection can be performed on the same chip.
As the second reaction part, a well-like reaction part may be provided, or a flow path may be provided (channel-like second reaction part), and the reaction may be performed in the flow path. For example, as shown in FIG. 3, a configuration having a plurality of openings 6 a and a flow path 26 connecting the plurality of openings 6 a is given as an example of the flow path-shaped second reaction section 6 ′. In this case, each opening diameter is about 5 mm or less, and the depth of the opening 6a is about 1 mm to 5 mm.

Moreover, you may form a flow path-shaped 2nd reaction part as follows. That is, as shown in FIG. 4, a plurality of through holes 30 are formed in the substrate 2. Then, as shown in FIG. 5, grooves 31 are formed on the back surface of the substrate 2 over the plurality of through holes 30. Further, as shown in FIG. 6, a bottom forming film 32 that covers the groove 31 is bonded to the back surface of the substrate 2. Thereby, the flow path 26 ′ is easily formed. The width and height of the groove 31 at this time are preferably in the range of 1 mm to 5 mm, respectively.
The groove 31 may be formed by connecting the through holes 30 with a straight line, or may have a bent shape to prevent evaporation of the reagent or the test object.

A film-like film can be used as the bottom forming film 32. Examples of such films include polyolefin films such as polyethylene, polypropylene and polymethylpentene, acrylic films such as polymethyl acrylate and polymethyl methacrylate, polystyrene films, polyacetal films, polyamide films, polyacrylonitrile films, polycarbonate films, Examples include olefin-based films, silicon resin-based films, and fluorine-based resin films. Moreover, you may use metal foil, such as aluminum, and the laminated film of metal foil and the above-mentioned resin film. The bottom forming film can be bonded using an adhesive. Moreover, you may bond together by heat sealing. A heat seal is preferable because it is not necessary to consider the influence of the adhesive in the reaction part.
Further, it is preferable that the bottom forming film 32 has a shape in which a part thereof bites into the groove 31. This is because there is no gap between the substrate 2 and the bottom forming film 32, and there is no leakage of reagents and test objects.

Furthermore, as shown in FIG. 7, the side cross section of the reagent storage unit 8 in the present embodiment is formed in two stages. That is, a large-diameter recess 11 having an opening 8a of the reagent storage 8 and a diameter substantially the same as the opening 8a is formed in the reagent storage 8. The peripheral wall portion of the large-diameter recess 11 is set to a predetermined height dimension. A small-diameter concave portion 12 is formed at the bottom of the large-diameter concave portion 11 so as to be directed deeper in the depth direction of the reagent containing portion 8 (thickness direction of the substrate 2). The small diameter recess 12 is set to have a smaller diameter than the large diameter recess 11.
The shape of the side cross section of the small-diameter recess 12 is not particularly limited, but the side part constituting the opening 12a is cylindrical and the bottom part is hemispherical or the whole is preferably hemispherical. If it is hemispherical, when the reagent is injected, it is possible to prevent scattering of the reagent and mixing of bubbles as much as possible. Moreover, it becomes excellent in the taking-out property at the time of taking out the injected reagent.
The small diameter recess 12 is provided with a first covering film (first lid member) 16 that covers the opening 12 a of the small diameter recess 12 and seals the inside of the small diameter recess 12. A second covering film (second lid member) 17 that covers the opening 11a and seals the inside of the large-diameter concave portion 11 is provided. Therefore, a clearance 20 having the same height as that of the large-diameter concave portion 11 is formed between the first covering film 16 and the second covering film 17.

The first and second coating films 16 and 17 have a two-layer structure in which a base material (hard aluminum layer) 14 is disposed in the upper layer and a seal layer 15 is disposed in the lower layer. The first and second coating films 16 and 17 are bonded and fixed to the bottom of the large-diameter recess 11 and the surface of the substrate 2 by the seal layer 15 disposed in the lower layer, and injected into the reagent storage unit 8. The liquid reagent 24 is sealed.
The base material 14 is made of hard aluminum, and has a thickness of 10 μm or less and an elongation of 1% / cm 2 or less. In addition, when a tubular object having a tip diameter of 0.9 mm and an inner diameter of 0.5 mm as viewed as a syringe needle or a disposable tip for dispensing is pierced from above in the vertical direction, the load is 0.5 kgf. The following is preferable.
On the other hand, the seal layer 15 is made of sheet-like acid component-modified PET (polyethylene terephthalate), which is a copolyester resin, and has a thickness of 15 μm or less. Furthermore, the load when pierced as described above is preferably 0.5 kgf or less.

  The first coating film 16 is individually provided in the small-diameter recess 12 of each reagent storage unit 8, and the second coating film 17 extends over each reagent storage unit 8 as shown in FIG. 1. One is provided. However, as shown in FIG. 8, if a large large-diameter concave portion 11 is formed so as to extend over each reagent storage portion 8, one first sheet is formed on the bottom of the large large-diameter concave portion 11 over each small-diameter concave portion 12. The covering film 16 can be provided, and one second covering film 17 can be provided in the opening 11a.

  Further, as another embodiment of the first and second coating films 16 and 17, in addition to the seal layer 15, a protective layer is provided on the upper surface of the base material 14, and the first and second coating films 16 and 17 are provided. A three-layer structure may be used. The protective layer can be made of an acrylic resin or polyester resin film having a thickness of 15 μm or less, an elongation of 30% / 180 mm or less, and a rupture degree of 2 kg / cm 2 or less. It is preferable to use mat PET, which is a brittle resin processed into a shape, because it can improve the piercing property with a needle such as a syringe. Note that the brittle resin is a resin that is weak against impact and has a so-called “brittle” property. In addition to a mat-like surface, for example, a foam-like resin may be used.

  As described above, when the first and second coating films 16 and 17 have a three-layer structure of the protective layer, the base material 14 and the seal layer 15, the first and second coating films 16 corresponding to the protective layer are used. , 17 increases, and the piercing property described above decreases. Therefore, a liquid sealant such as a heat seal lacquer is applied as the seal layer 15 so as to have a thickness of 5 μm or less, and the thickness of the hard aluminum of the substrate 14 is 10 μm or less, preferably 8 μm. Set as follows. By doing in this way, the 1st and 2nd coating films 16 and 17 can be made into 3 layer structure, without reducing piercing property.

In addition, it is preferable that the opening diameter of the reagent accommodating part 8 exists in the range of 1-50 mm.
The amount of the reagent is from a very small amount of about several hundred nl to about several hundred μl, and the diameter of a general dispensing needle is about several tens μm to several mm. For this reason, in consideration of appropriate dispensing and target volume, the opening diameter of the reagent storage unit 8 is preferably in the range of 1 to 50 mm.
Moreover, it is preferable that the depth of the reagent accommodating part 8 exists in the range of 1-50 mm.
Moreover, the material of the 1st and 2nd coating films 16 and 17 can be changed suitably. For example, polyolefin films such as polyethylene, polypropylene and polymethylpentene, acrylic films such as polymethyl acrylate and polymethyl methacrylate, polystyrene films, polyacetal films, polyamide films, polyacrylonitrile films, polycarbonate films, cycloolefin films, silicone resins Examples thereof include a fluorine-based film and a fluorine-based resin film. Moreover, you may use metal foil, such as aluminum, and the laminated film of metal foil and the above-mentioned resin film. These film-like lid members can be bonded together using an adhesive. As the adhesive, a heat-resistant curable adhesive can be used. Further, it may be bonded by heat sealing. A heat seal is preferable because it is not necessary to consider the influence of the adhesive on the reagent.

Further, as shown in FIG. 9, a convex portion 21 may be provided around the opening 8 a of the reagent storage unit 8 as in the case of the reaction unit 6. The convex portion 21 is preferably a cylindrical shape raised from the entire circumference of the opening 8a in order to prevent the reagent 24 from being scattered as much as possible. By providing the convex portion 21, the first and second coating films 16 and 17 can be easily bonded together.
In particular, when pasting together by heat sealing, it is only necessary that the portion to be heated is in contact with the convex portion 21 instead of the entire base material 14, so that the thermal influence on the reagent in the reagent storage portion can be reduced.
As such a convex part 21, it is preferable that the width is within a range of 0.1 to 3 mm and the height is within a range of 0.05 to 2 mm.

Further, as shown in FIG. 10, a cylindrical convex portion 21 having a diameter larger than the opening diameter of the opening portion 8 a of the reagent storage portion 8 may be provided. In this way, the side cross section of the reagent container 8 can be formed in a substantially hemispherical shape, and the clearance 20 can be easily formed while simplifying the configuration of the reagent container 8.
Moreover, although the reagent storage part 8 was made into a well shape, it is not restricted to this, You may make it provide a flow path.

Next, the operation of the reagent container 1 in this embodiment will be described.
Here, a case where DNA detection is performed will be described.
A liquid reagent 24 which is a sample DNA reagent is stored in the reagent storage unit 8. Then, the injection needle is passed through the first and second coating films 16 and 17. At this time, the first and second coating films 16 and 17 are torn radially outward of the injection needle, and the periphery of the through hole formed in the first and second coating films 16 and 17 and the outer periphery of the injection needle A gap is created between the surface. This gap ensures the gas flow inside and outside the reagent storage unit 8.

  Next, a part of the reagent 24 is aspirated and separated, and then the injection needle is pulled out. And the reagent 24 is inject | poured in the reaction part 6 which has arrange | positioned the nucleic acid probe beforehand from the injection needle. Then, the presence or absence of a reaction between the reagent 24 and the nucleic acid probe is detected. At this time, the presence or absence of a reaction can be detected by attaching a labeling substance to the sample DNA. If a plurality of reaction units 6 are provided and nucleic acid probes each having a different sequence are arranged, the sequence of the sample DNA can be detected efficiently. Moreover, it can be used for the analysis of single nucleotide polymorphism (SNP) in DNA reaction. In this case, there may be a plurality of nucleic acid probes and other reagents used for detection, and one of those reagents only needs to be labeled. The SNP analysis may use an invader method developed by Third Wave Technologies, Inc. (Madison, Wisconsin, USA).

  Here, conventionally, as shown in FIG. 11, when the reagent 24 is dispensed by the injection needle 35, that is, when the injection needle 35 is put into the reagent 24, or when the reagent 24 is aspirated by the injection needle 35, or When the injection needle 35 that has sucked the reagent 24 is pulled out, the reagent 24 may be scattered to the outside of the accommodating portion 36. In addition, when the reagent 24 is discharged from the injection needle 35 and the reagent 24 is injected into the housing portion 36 or the like, the reagent 24 may be scattered.

  In the reagent container 1 in the present embodiment, scattering of the reagent 24 is prevented as follows. That is, as shown in FIG. 12, when the reagent 24 is injected or dispensed through the injection needle through the first and second coating films 16 and 17, the small-diameter concave portion 12 is formed into the first coating film. Therefore, the movement of the droplets of the reagent 24 accommodated in the small-diameter recess 12, that is, the movement to leak out of the small-diameter recess 12 is restricted. Furthermore, even if the reagent 24 leaks into the large-diameter recess 11 that is outside the small-diameter recess 12, the large-diameter recess 11 is sealed by the first coating film 16. That is, the movement of the reagent 24 in the clearance 20 leaking out of the reagent storage unit 8 is restricted.

  As described above, according to the reagent container 1 of the present embodiment, the movement of the reagent 24 leaking out of the reagent storage unit 8 is restricted as described above. Can be prevented from being scattered. Therefore, the injection of the reagent into the reagent storage unit 8 and the sorting of the reagent from the reagent storage unit 8 can be performed easily and appropriately, and the test can be performed quickly and with high accuracy.

In addition, when the injection needle is inserted into the first and second coating films 16 and 17, the gas flow is ensured by the gap. Therefore, when the reagent 24 is aspirated from the injection needle, the air flows through the gap. On the other hand, when the reagent 24 is discharged from the injection needle, the air can flow out through the gap. Therefore, air can flow in and out through the gap according to the pressure in the reagent storage unit 8, and the reagent 24 can be sucked and discharged from the injection needle easily and quickly. Furthermore, the injection needle can be easily pierced.
Further, since the clearance 20 is formed, the reagent 24 leaked from the small-diameter recess 12 can be retained, and the reagent can be reliably prevented from scattering out of the reagent storage unit 8.

In the present embodiment, the first and second coating films 16 and 17 can be provided in the reagent storage unit 8. However, the present invention is not limited to this and may be provided in the reaction unit 6. In this case, the reaction part 6 functions as a recessed part or a flow path.
Moreover, although the reaction part 6, the detection part 7, and the reagent storage part 8 were provided in one board | substrate 2, it is not restricted to this, You may make it provide in a respectively separate board | substrate.
Moreover, although the board | substrate 2 was made into the rectangular plate shape, it is not restricted to this, The shape can be changed suitably. For example, the container may be filled with various shapes such as tubes and bottles, and the openings may be covered with the first and second coating films 16 and 17.
The technical scope of the present invention is not limited to the above embodiment, and various modifications can be made without departing from the spirit of the present invention.

It is a perspective view which shows embodiment of the container for reagents which concerns on this invention. It is a sectional side view which shows a mode that the convex part was provided in the reaction part of this embodiment. It is a sectional side view which shows the modification of the reaction part of this embodiment. It is a figure explaining the formation process of a flow path, Comprising: It is a sectional side view which shows a mode that the through-hole was provided in the board | substrate. It is a figure explaining the formation process of a flow path, Comprising: It is a sectional side view which shows a mode that the groove | channel was provided in the back surface of the board | substrate of FIG. It is a figure explaining the formation process of a flow path, Comprising: It is a sectional side view which shows a mode that the film for bottom part formation was provided in the groove | channel of FIG. It is a sectional side view which expands and shows the reagent accommodating part of FIG. It is a perspective view which shows the modification of the reagent accommodating part of FIG. It is a sectional side view which shows a mode that the convex part was provided in the reagent accommodating part of FIG. FIG. 10 is a side sectional view showing a modification of the reagent storage unit of FIG. 9. It is a figure which shows the accommodating part of the conventional reagent container, Comprising: It is explanatory drawing which shows a mode that the reagent scattered. It is explanatory drawing which shows a mode that a reagent is inject | poured and fractionated in the reagent storage part of this embodiment.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Reagent container 6 Reaction part 8 Reagent accommodating part (recessed part) 11 Large diameter recessed part 12 Small diameter recessed part 14 Base material (hard aluminum layer) 15 Seal layer 16 1st coating film (1st cover member) 17 2nd coating film (Second lid member) 20 Clearance 24 Reagent 26 Flow path

Claims (5)

A recess or flow path into which the reagent is injected;
A first lid member covering the recess or the opening of the flow path;
A second lid member that covers the opening through the first lid member;
Bei to give a,
A clearance is provided between the first lid member and the second lid member;
The concave portion includes a large-diameter concave portion constituting an opening of the concave portion, and a small-diameter concave portion provided at the bottom of the large-diameter concave portion and set to have a smaller diameter than the large-diameter concave portion. A reagent container, wherein the first lid member is provided, and the second lid member is provided in the large-diameter recess . Around the opening of the concave portion, a convex portion raised upward is provided,
The reagent container according to claim 1, wherein the second lid member is provided so as to be in contact with the convex portion. A plurality of the recesses,
The reagent container according to claim 2, wherein the convex portions provided around the openings of the plurality of concave portions are connected to each other at the same height. The reaction portion is a plurality of through holes formed in the substrate, a groove portion formed on one surface of the substrate over the plurality of through holes, and a film that is bonded to the one surface of the substrate and covers the groove portion The reagent container according to any one of claims 1 to 3, wherein the reagent container is configured as follows. The reagent container according to any one of claims 1 to 4, wherein the first lid member is a film including a hard aluminum layer and a seal layer.

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