JP4987150B2 - Composition containing β-glucan - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a novel composition useful as a noodle quality improving agent for improving the texture of noodles, and a method of producing the same. <P>SOLUTION: The composition contains 9-9.99 mass% &beta;-1,3-1,4-glucans and 0.1-50 mass% arabinoxylan. The method of producing the composition includes: a step A for crushing barley containing at least 50 mass% endosperm portion so that particles of 500 &mu;m or larger in particle size occupy not more than 10 vol.% and that particles of 40 &mu;m or smaller in particle size occupy 30-95 vol.%; and a step B for obtaining a section where particle of 50-500 &mu;m in particle size occupy at least 80 vol.% from the crushed product obtained in the step A. <P>COPYRIGHT: (C)2012,JPO&amp;INPIT

Description

  The present invention relates to a novel composition, and more particularly to a novel composition useful as a noodle quality improver for improving the texture of noodles and a method for producing the same. The present invention further relates to a method for concentrating β-1,3-1,4-glucan and arabinoxylan simultaneously.

β-glucans are polysaccharides widely distributed in gramineous plants such as barley, and are substances that have hitherto been attracting attention for health functional properties such as intestinal function as dietary fiber, among which β-1,3-1 , 4-glucan has attracted attention because it has functions such as an immune enhancing action and a blood glucose level suppressing action. However, the β-1,3-1,4-glucan content in natural barley was about 5% by mass.
For these reasons, a composition containing β-1,3-1,4-glucan at a high concentration has been conventionally obtained. For example, Patent Document 1 describes a composition having a β-glucan content of 3%, which is obtained by extracting hot water from glutinous bare barley that is generated from mash. From the analysis method and the like, this β-glucan is almost β-1,3-1,4-glucan.
Patent Document 1 describes a composition having a content of 91% β-glucan (approximately β-1,3-1,4-glucan) obtained by further adding ethanol and precipitating.

However, when extracting from barley, the phase containing β-1,3-1,4-glucan contains almost no arabinoxylan contained in barley. A composition containing 4-glucan and arabinoxylan at a high concentration at the same time has not been known.
Further, β-glucan is known to have an effect of improving the quality of processed foods containing starch as a main component. Patent Document 2 discloses β-glucan extracted from barley (approximately β-1,3-1,4-glucan). Contains udon that has a luster and firmness and is excellent in taste, texture and appearance.
However, the elasticity, which is regarded as most important as the texture of noodles, was slightly improved due to its elasticity, but it did not provide excellent elasticity.

JP 2002-241784 A JP 2002-306094 A

Accordingly, an object of the present invention is to provide a composition containing β-1,3-1,4-glucan and arabinoxylan at a high concentration at the same time.
Another object of the present invention is to provide a noodle quality improving agent capable of imparting excellent elasticity to noodles.
Another object of the present invention is to provide a method for producing the composition and the noodle quality improver.
Another object of the present invention is to provide a method for concentrating β-1,3-1,4-glucan and arabinoxylan simultaneously.

The present invention includes a step A for pulverizing barley so that particles having a particle size of 500 μm or more are 10% by volume or less and particles having a particle size of 40 μm or less are 30 to 95% by volume, and a pulverized product obtained in the step A The composition obtained by the manufacturing method which has the process B which obtains the fraction whose particle diameter of 50-500 micrometers is 80 volume% or more from, Comprising : ( beta) -1,3-1,4-glucan is 9-9 The object is achieved by providing a composition characterized by containing 99.9% by mass and arabinoxylan in an amount of 0.1-50% by mass.

  Further, the present invention provides a composition in which the composition ratio of arabinose and xylose constituting arabinoxylan is arabinose: xylose = 1: 0.5 to 1: 5 (molar ratio) in the above composition. The above objective has been achieved.

The effect of the present invention is to provide “ a composition containing β-1,3-1,4-glucan and arabinoxylan at a high concentration simultaneously ” .

The composition of the present invention will be described.
The composition of the present invention contains 9 to 99.9% by mass of β-1,3-1,4-glucan and 0.1 to 50% by mass of arabinoxylan.
The β-1,3-1,4-glucan is not particularly limited as long as it is a β-glucan having a β-1,3-glucoside bond and a β-1,4-glucoside bond. It may be derived from plants, basidiomycetes, or microorganisms.

The arabinoxylan is not particularly limited, and may be any arabinoxylan having an arabinose side chain or an arabinose polymerization side chain in the polymerization main chain of xylose, but preferably constitutes an arabinoxylan. The constituent ratio of arabinose and xylose is arabinose: xylose = 1: 0.5 to 1: 5 (molar ratio), more preferably 1: 1 to 1: 3.5.
If the ratio of xylose to arabinose is too small, it becomes difficult to obtain the effect of improving the noodle quality due to the synergistic effect with β-1,3-1,4-glucan when the composition of the present invention is used as a noodle quality improver. If it is too much, the water solubility is lowered, and it becomes difficult to obtain the effect of improving the noodle quality.
Although the origin of the arabinoxylan is not particularly limited, for example, since it is contained in a lot of gramineous plants, it is preferably derived from a gramineous plant.

  The composition of the present invention only needs to contain the above β-1,3-1,4-glucan and arabinoxylan within the above amounts, and β-1,3-1,4-glucan and arabinoxylan. However, it is preferable to obtain the composition of the present invention from a raw material containing β-1,3-1,4-glucan and arabinoxylan at the same time.

For example, as a gramineous plant containing a large amount of β-1,3-1,4-glucan, rye, rye wheat, rice, wheat, barley, oats, barnyard millet, millet, corn and the like can be exemplified. Β-1,3-1,4-glucan can be obtained from the seed seed skin, seed seed coat (coffin), cob, stem, leaf, embryo, endosperm, and the like. Seed bran is so-called bran or bran. Seed shell is burdock, cob is corn cob, stem is inowara, wheatwara, etc.
Among these, the endosperm portion of barley having a high β-1,3-1,4-glucan content is particularly useful for obtaining β-1,3-1,4-glucan, which is a constituent of the composition of the present invention. Is suitable.
As an endosperm part of barley, what barley grain was shaved from the outer peripheral part and it was 90 mass%, Preferably what was 80% mass or less is good. Further, it is preferable to use a barley variety having a high β-1,3-1,4-glucan content as a raw material. Variants such as Godiva, Benihadaka, Uruchi H-HOR 4097/66, Nepa 11684, Riso 86, Four R series 1164, and derived varieties based on these can be used.

On the other hand, the same raw materials as in the case of β-1,3-1,4-glucan can be exemplified as those suitable for obtaining arabinoxylan and containing a large amount of arabinoxylan.
Of these, one of six varieties of “Kashimamugi” or edible varieties of Rojo barley, which is high in β-1,3-1,4-glucan and arabinoxylan and is stably produced as a raw material for barley tea and the like. “Minolimui” is suitable as a raw material.

Next, the manufacturing method of the composition of this invention which manufactures the above-mentioned composition of this invention is demonstrated.
As described above, in the composition of the present invention, β-1,3-1,4-glucan and arabinoxylan are separately prepared by a conventional method, and if necessary, concentrated by a conventional method so that the above ratio is obtained. However, according to the method of the present invention described below, the composition of the present invention can be easily produced.
In addition, β-1,3-1,4-glucan and arabinoxylan are separately prepared by a conventional method, and if necessary, concentrated by a conventional method and mixed so that the above ratio is obtained. Can be optionally added components other than β-1,3-1,4-glucan and arabinoxylan, but when the composition of the present invention is used as a noodle quality improver, It is preferable to add a raw material, such as starch or the above-mentioned, for example, barley pulverized powder, bran or bran to obtain β-1,3-1,4-glucan. When these optional components are added, β-1,3-1,4-glucan and arabinoxylan contained therein are included in the content of the composition of the present invention.

  In the method for producing the composition of the present invention, barley containing at least 50% by mass of the endosperm portion is 10% by volume or less, preferably 5% by volume or less, more preferably 0 and the particle size of particles having a particle size of 500 μm or more. Step A for pulverizing particles having a particle size of 40 μm or less to 30 to 95% by volume, preferably 40 to 70% by volume, more preferably 48 to 60% by volume, and a particle size of 50 from the pulverized product obtained in Step A Step B in which particles of ˜500 μm obtain a fraction of 80% by volume or more, preferably 90% by volume or more, more preferably 99% by volume or more.

First, barley, preferably “Kashimamugi” and / or “Minolimugi” is pulverized. As a raw material in that case, at least the endosperm portion is 50% by mass or more, preferably 70% by mass or more, more preferably 90% by mass or more. Use raw barley ingredients. The content of the endosperm part is not the endosperm part content in each barley grain itself, but the ratio of the whole raw material used.
The barley pulverization method may be in accordance with a conventional method. For example, the barley may be pulverized using a roller pulverizer, an impact pulverizer, a hammer mill (pulverizer), a mortar pulverizer, or the like.

  Normally, β-glucan and arabinoxylan are distributed throughout the barley grain and are present as cell wall components in endosperm. The present inventors examined the optimal pulverized form for obtaining a fraction containing a high amount of β-glucan or arabinoxylan by conducting sieving fractionation, measurement with a particle size distribution meter, microscopic observation, etc. It was observed that starch granules having a size in the range of 5 to 40 μm were contained, and the cell wall was surrounded so as to contain several tens to several hundreds of starch granules. When barley grains were pulverized using a laboratory coffee mill, the barley grains were refined with the pulverization time, and a mass of several to several hundred endosperm cells of about 500 μm was observed at the initial stage of pulverization. When the size is about 100 μm, the cell wall is crushed, and a lump in which the cell wall and starch granules are bound is observed. Finally, the cell wall is refined to the same size as the starch granules, and a uniform size of several tens of μm is obtained. It became fine powder. In the course of the experiment, the present inventors have made β-1,3-1, by separating only the starch granules from the cell wall and sieving it under certain pulverization conditions without refining the cell wall. It was found that cell wall components and starch granules containing a large amount of 4-glucan and arabinoxylan can be separated, and as a result, β-1,3-1,4-glucan and arabinoxylan can be concentrated.

The above specific pulverization conditions are 10% by volume or less of particles having a particle diameter of 500 μm or more, preferably 5% by volume or less, more preferably 0 and 30 to 95% by volume of particles having a particle diameter of 40 μm or less, preferably It grind | pulverizes so that it may become 40-70 volume%, More preferably, it is 48-60 volume%. The fractionation condition is to obtain a fraction having a particle size of 50 to 500 μm of 80% by volume or more, preferably 90% by volume or more, more preferably 99% by volume or more from the pulverized product obtained in the pulverization step. is there.
If the pulverization is insufficient, a large amount of unnecessary starch or the like is mixed during fractionation, and the concentrations of β-1,3-1,4-glucan and arabinoxylan are lowered.
Further, if it is pulverized more than necessary, the particles containing β-1,3-1,4-glucan and arabinoxylan are further refined and β-1,3-1,4-glucan obtained by fractionation. And the amount of arabinoxylan decreases, and industrial suitability decreases.

After pulverizing the barley as described above, particles having a particle diameter of 50 to 500 μm may be fractionated from the obtained pulverized product. The fractionation method is not particularly limited. For example, fractionation is performed by sieving or airflow classification usually used for cereal classification, or in sieving fractionation, for example, sieving fractionation for a certain time using a screen with a desired roughness. Then, the fraction on the screen may be collected.
In this way, particles having a particle size of 50 to 500 μm may be obtained, but there is no problem even if particles having a particle size outside the range of 50 to 500 μm are mixed. What is necessary is just to obtain a fraction having the above proportion of particles of ˜500 μm.
The obtained fraction may be pulverized or finely pulverized to a desired particle size by a conventional method as necessary.

Next, the noodle quality improving agent of the present invention will be described.
The noodle quality improving agent of the present invention comprises the above-described composition of the present invention. That is, it is a noodle quality improving agent containing 9 to 99.9% by mass of β-1,3-1,4-glucan and 0.1 to 50% by mass of arabinoxylan.
The content of β-1,3-1,4-glucan in the noodle quality improving agent of the present invention is 9 to 99.9% by mass, preferably 10 to 80% by mass. When the content of β-1,3-1,4-glucan is less than the above amount, the effect of improving the noodle quality of the present invention to give excellent elasticity is insufficient. In particular, since the amount of arabinoxylan is reduced, a synergistic effect with arabinoxylan in the noodle quality improvement effect cannot be obtained, and the noodle quality improvement effect of the present invention that provides excellent elasticity is insufficient.

The content of arabinoxylan in the noodle quality improving agent of the present invention is 0.1 to 50% by mass, preferably 1 to 20% by mass. If the content of arabinoxylan is less than the above amount, a synergistic effect with β-1,3-1,4-glucan in the effect of improving the noodle quality of the present invention that gives excellent elasticity cannot be obtained, and the amount exceeds the above amount. However, the excellent elasticity is also lowered, stickiness appears, and the texture is also lowered.
In addition, the above arabinoxylan has a composition ratio of arabinose and xylose constituting arabinoxylan from the viewpoint of more prominently obtaining a synergistic effect with β-1,3-1,4-glucan in the effect of improving noodle quality. A ratio of 1: 0.5 to 1: 5 (molar ratio) is preferable, and a ratio of 1: 1 to 1: 3.5 is more preferable.
The method for producing the noodle quality improving agent of the present invention is not particularly limited, and is the same as the method for producing the composition of the present invention described above.

  In addition, the noodle quality improving agent of the present invention includes, if necessary, a pH adjuster, an emulsifier, an antioxidant, a polysaccharide (when arabinoxylan is included, it is included as an essential arabinoxylan in the present invention), starches, enzymes Kinds, protein materials derived from plants and animals, microorganisms, quality stabilizers, and the like can be added within a range that does not impair the effects of the present invention.

  Examples of the pH adjuster include phosphates such as monosodium phosphate, disodium phosphate, trisodium phosphate, sodium pyrophosphate, sodium polyphosphate, sodium tripolyphosphate, potassium pyrophosphate, sodium hexametaphosphate, metaphosphoric acid. Sodium, potassium metaphosphate, and various other organic acids such as acetic acid, lactic acid, citric acid, gluconic acid, succinic acid, tartaric acid, fumaric acid, ascorbic acid, acidic pyrophosphoric acid, chlorogenic acid, malic acid, and the like Examples of the salt and alkali agent substance include sodium hydrogen carbonate, sodium carbonate, hydrogen hydrogen carbonate, potassium carbonate, ammonium hydrogen carbonate and the like, which are commonly used pH adjuster substances. Can be used with more than seeds.

  Examples of the emulsifier include glycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, organic acid monoglyceride, acetic acid monoglyceride, polyglycerin fatty acid ester, polyglycerin condensed ricinoleic acid ester, propylene glycol fatty acid ester, stenaroyl calcium lactate, polyoxyethylene. Examples of commonly used emulsifiers such as sorbitan fatty acid ester, lecithin, and enzyme-treated lecitene can be exemplified, and these can be used alone or in combination of two or more.

  Examples of the antioxidant include tocopherol, L-ascorbine koji, erythorbates, catechins, tannins, anthocyanins, polyphenols such as tea polyphenols, L-ascorbic acid stearate, sodium L-ascorbate, etc. These can be used alone or in combination of two or more.

  Examples of the polysaccharide include apple fiber, alginic acid, sodium alginate, propylene gallic ester of alginate, gum arabic, inulin, cassia gum, karaya gum, agar, curdlan, carrageenan, carboxymethylcellulose (CMC), carboxymethylcellulose calcium, xanthan gum, chitin , Chitosan, guar gum, gel comannan, corn fiber, chondroitin, psyllium seed coat, gellan gum, water soluble soy polysaccharide, cellulose, tamarind seed polysaccharide, soy dietary fiber, tara gum, togant gum, hemicellulose, hyaluronic acid, beet fiber, fine cellulose, pullulan , Fur celeran, fucoidan, fructan, pectin, mannan, mucin, levan, locust bean gum, and this Can be exemplified such as decomposition product, it can be used one or two or more thereof.

  Examples of the starches include tapioca starch, potato starch, corn starch, waxy corn starch, rice starch, and barley starch (barley starch includes those contained in the production of the noodle quality improving agent of the present invention), sago Examples include starch, high amylose corn starch, and mung bean starch. Processed starch can also be used as starches, for example, bleached starch, soluble starch, cross-linked starch, esterified starch, etherified starch, esterified cross-linked starch, etherified cross-linked starch, starch degradation product, etc. Can do. Moreover, these starches can use 1 type (s) or 2 or more types.

  Examples of the enzymes include amylase, invertase, catalase, cellulase, xylanase, papain, protease, pectinase, lysozyme, rivase, trypsin, pancreatin, bromelain, pepsin, beptidase, actininin and the like. Species or two or more can be used.

  Examples of protein materials derived from animals, plants and microorganisms include whole egg powder, egg white and yolk powder, milk protein, soybean protein, wheat isolate protein, plasma protein, pea protein, yeast extract, albumin such as whey, casein sodium, casein Examples include phosphopeptide (CPP), gelatin, soluble collagen, bonito node peptide, sardine peptide, casein decapeptide, and the like, and one or more of these can be used.

  Examples of the quality stabilizer include cyclic oligosaccharides, dextrin, indigestible dextrin, cyclofructan, difructose, glucosamine, sodium carbonate, sodium malate, propylene glycol, cyclotextrin, calcium salts such as calcium chloride and calcium citrate. , Calcium gelconate, calcium glycerophosphate, calcium acid pyrophosphate, calcium hydroxide, calcium carbonate, calcium lactate, calcium sulfate, primary calcium phosphate, secondary calcium phosphate, tertiary calcium phosphate, calcium buntothenate, calcium pyrohydrogen phosphate, coral calcium, Dolomite, eggshell calcium, beef bone meal calcium, scallop shell calcium, milk calcium, irons, such as ferric chloride, iron citrate, Ammonium iron, sodium iron citrate succinate, iron lactate, ferrous pyrophosphate, ferric pyrophosphate, ferrous sulfate, ferrous gelconate, heme iron, liver powder, water-soluble sugars (glucose, fructose, Sucrose, lactose, isomerized sugar, xylose, trehalose, coupling sugar, palatinose, reduced palatinose, sorbose, reduced starch saccharified starch, maltose, erythritol, reduced xylooligosaccharide, lactulose, fructooligosaccharide, galactooligosaccharide, etc.), sugar alcohols ( Xylitol, maltitol, mannitol, sorbitol, polydextrose), oils and fats such as palm oil, coconut oil, soybean oil, and cottonseed oil can be exemplified, and one or more of these can be used.

Next, the noodles of the present invention will be described.
The noodles of the present invention contain the above-described noodle quality improving agent of the present invention in an amount of 0.1 to 45% by mass, preferably 1 to 20% by mass, based on the solid content. If the amount of the noodle quality improving agent is less than the above, the effect of the noodle quality improving agent of the present invention to give excellent elasticity to the noodles is insufficient, and if used in excess of the above amount, the noodle itself is hard. Tend to be.

Next, a method for concentrating β-1,3-1,4-glucan and arabinoxylan of the present invention at the same time will be described.
The method of simultaneously concentrating β-1,3-1,4-glucan and arabinoxylan of the present invention comprises barley containing at least an endosperm portion of 50% by mass or more, preferably 70% by mass or more, more preferably 90% by mass or more. Particles having a particle size of 500 μm or more are 10% by volume or less, preferably 5% by volume or less, more preferably 0 and particles having a particle size of 40 μm or less are 30 to 95% by volume, preferably 40 to 70% by volume, more preferably. Step A for pulverization to 48 to 60% by volume, and particles having a particle diameter of 50 to 500 μm from the pulverized product obtained in Step A are 80% by volume or more, preferably 90% by volume or more, more preferably 99% by volume. % To obtain a fraction of at least%.
This concentration method of the present invention is the same as the above-described method for producing the composition of the present invention and the method for producing the noodle quality improving agent of the present invention.

  EXAMPLES The present invention will be further described below with reference to examples, but the present invention is not limited to these examples.

[Method for measuring β-1,3-1,4-glucan content]
For the measurement of β-1,3-1,4-glucan content, the McCleary method (enzyme method) was adopted. Specifically, a β-glucan measurement kit (model number K-BGLU) (manufactured by Megazyme) was used. First, the moisture content of a measurement sample applied to a 500 μm (30 mesh) sieve was measured in advance (infrared moisture meter, model FD-230, manufactured by Kett). 10 mg of this measurement sample was taken in a 17 ml tube, and 200 μl of 50% (v / v) ethanol solution was added and dispersed. Next, 4 ml of 20 mM phosphate buffer (pH 6.5) was added and mixed well, and then heated in a boiling water bath for 1 minute. Mix well and heat in hot water bath for another 2 minutes. After cooling to 50 ° C. and allowing to stand for 5 minutes, add 200 μl (10 U) of the lichenase enzyme solution (diluted with 20 ml of 20 mM phosphate buffer, the remaining amount is stored frozen) to each tube, The reaction was carried out at 50 ° C. for 1 hour. 5 ml of 200 mM acetate buffer (pH 4.0) was added to the tube and mixed gently. It was left to stand at room temperature for 5 minutes, and the supernatant was obtained by centrifugation. Take 100 μl of the supernatant in three tubes, one with 100 μl 50 mM acetate buffer (pH 4.0) and the other two with 100 μl (0.2 U) β-glucosidase solution (vials supplied with the kit) Was diluted with 20 ml of 50 mM acetate buffer and the remaining amount was stored frozen), and reacted at 50 ° C. for 10 minutes. 3 ml of glucose oxidase / peroxidase solution was added and reacted at 50 ° C. for 20 minutes, and the absorbance (EA) at 510 nm of each sample was measured. The β-1,3-1,4-glucan content was determined by the following formula.
β-1,3-1,4-glucan content (%, W / W) = (EA) × (F / W) × 8.46
F = (100) / (absorbance of glucose 100 μg)
W = calculated anhydride weight (mg)

[Measurement method of arabinoxylan content]
The arabinoxylan content was measured by ion-exchange chromatography and pulsed amperometry detection (HPAE-PAD) of arabinose and xylose liberated by acid hydrolysis of the polysaccharide obtained by solvent precipitation.
Weigh accurately 1 mg of the dried powder sample, add 1 mL of 70% (v / v) ethanol, stir at room temperature for 30 minutes, and then cool at 4 ° C. for 1 hour. The supernatant is removed by centrifugation and the resulting precipitate is lyophilized. Add 0.1 mL of 72% (v / v) sulfuric acid to the dried sample, stir, and ice-cool for 1 hour. Add 0.8 mL of water and heat at 100 ° C. for 4 hours. Neutralize by adding 50 mg of powdered BaCO ₃ , and centrifuge to collect the supernatant. Demineralize with ion exchange resin (Dowex 50-X80 H ⁺ type) and freeze-dry. An aqueous solution in which 1 mL of distilled water was added to the dried sample and completely dissolved to release monosaccharides, and this was diluted 1000 times with ultrapure water was used as an analysis sample.
Monosaccharides were quantified using an ion exchange chromatography apparatus (Dionex) under the following analysis conditions. The column was CarboPac PA1 (manufactured by Dionex), and the detector was a pulsed amperometry detector (manufactured by Dionex) with the working electrode being gold, with a flow rate of 1.0 mL / min. Column temperature of 35 ° C., 100 mM NaOH aqueous solution as eluent A, and ultrapure water as eluent B, eluent A from an initial concentration of 20% by mass to a final concentration of 35% by mass, 0.75% by mass / min. A gradient with a linear gradient of An aqueous solution in which L (+)-Arabinose (010-04582, manufactured by Wako Pure Chemical Industries, Ltd.) and D (+)-Xylose (244-00302, Wako Pure Chemical Industries, Ltd.) is 1.0 μg / mL with ultrapure water is standard. The product was quantified from the elution time and peak area value when 50 μL of the analysis sample was injected. The total content of arabinose and xylose detected was defined as the arabinoxylan content, and the composition ratio was calculated from each content.

[Measurement method of particle size distribution]
The particle size distribution was performed with a laser diffraction particle size distribution analyzer SALD-2100 (manufactured by Shimadzu Corporation). In 150 mL of the dispersion solvent EtOH, a sufficiently dried sample is added so that the absorbance after ultrasonic irradiation becomes 0.1, and ultrasonic irradiation is performed for 1 minute. The particle diameter detected by convection with stirring and the relative particle amount (volume ratio) were measured.

[Example 1]
After pulverizing commercial barley (pressed barley) grains (endosperm partial content of 100% by mass) for 1 minute with a commercially available electric coffee mill, set the particle size knob of the pulverizer (trade name: Micro Powder, manufactured by West Co., Ltd.) to 45 microns. Then, the pulverized product in the coffee mill was further pulverized to obtain 100 g of barley pulverized product A.
In the barley pulverized product A, particles having a particle diameter of 500 μm or more were 1% by volume, and particles having a particle diameter of 40 μm or less were 51.8% by volume.
The barley pulverized product A was treated for 45 minutes with an analytical sieve of 100 mesh (mesh diameter 150 μm), and a sieve ON product 15 g (particles having a particle diameter of 50 to 500 μm was a fraction of 96.3 vol%). ) This was further pulverized by setting the particle size knob of a pulverizer (trade name: Micro Powder, manufactured by West Co., Ltd.) to 10 microns. It was set to 1.
Sample No. 1 had a β-1,3-1,4-glucan content of 11.7% by mass and an arabinoxylan content of 1.2% by mass. The composition ratio of arabinose and xylose constituting arabinoxylan was arabinose: xylose = 1: 2.5.

[Example 2]
The barley grains (endosperm partial content: 100% by mass) made from oak from Ibaraki Prefecture in 2004 were shaved from the outer periphery and the fine ratio was 60%, and pulverized barley B was obtained.
In the barley grind B, particles having a particle size of 500 μm or more were 0% by volume, and particles having a particle size of 40 μm or less were 53.9% by volume.
2 kg of the barley pulverized product B was treated with a 140 mesh sieve for 60 minutes to obtain 440 g of a sieve ON product (particles having a particle diameter of 50 to 500 μm were a fraction of 95.6% by volume). This was further pulverized by setting the particle size knob of a pulverizer (trade name: Micro Powder, manufactured by West Co., Ltd.) to 10 microns. 2.
Sample No. 2 had a β-1,3-1,4-glucan content of 13.2% by mass and an arabinoxylan content of 4.2% by mass. The composition ratio of arabinose and xylose constituting arabinoxylan was arabinose: xylose = 1: 1.6.

[Comparative Example 1]
Particle size of 84 g of OFF product that passed through the sieve in Example 1 (particles having a particle size of 50 to 500 μm was a fraction of 41.9% by volume) was pulverized by a pulverizer (trade name: Micropowder, manufactured by West Co., Ltd.). Set the knob to 10 microns and grind it further. It was set to 1.
Comparative sample No. 1 had a β-1,3-1,4-glucan content of 2.5 mass% and an arabinoxylan content of 2.7 mass%. The composition ratio of arabinose and xylose constituting arabinoxylan was arabinose: xylose = 1: 4.5.

[Comparative Example 2]
Particle size of OFF product 1540 g (particles having a particle size of 50 to 500 μm was a fraction of 36.8% by volume) that passed through the sieve in Example 2 was pulverized (trade name: Micro Powder, manufactured by West Co., Ltd.). Set the knob to 10 microns and grind it further. 2.
Comparative sample No. 2 had a β-1,3-1,4-glucan content of 2.8% by mass and an arabinoxylan content of 3.9% by mass. The composition ratio of arabinose and xylose constituting arabinoxylan was arabinose: xylose = 1: 4.3.

[Comparative Example 3]
In 2004, the barley grains produced in Ibaraki Prefecture were shaved from the outer periphery, and 50 g of barley grains having a fine ratio of 60% were ground in a commercially available electric coffee mill for 10 minutes to obtain barley ground product C.
In the barley pulverized product C, particles having a particle size of 500 μm or more were 30% by volume, and particles having a particle size of 40 μm or less were 42.3% by volume.
30 g of barley pulverized product C was treated with a 140 mesh sieve for 60 minutes to obtain 25 g of a sieve ON product (particles having a particle diameter of 50 to 500 μm were a fraction of 61.4% by volume). This was pulverized by setting the particle size knob of a pulverizer (trade name: Micro Powder, manufactured by West Co., Ltd.) to 135 microns, then set to 10 microns and further pulverized. It was set to 3.
On the other hand, 5 g of the OFF product that passed through the node as described above (particles having a particle diameter of 50 to 500 μm was a fraction of 38.3% by volume) was pulverized by a pulverizer (trade name: Micro Powder, manufactured by West Co., Ltd.) After setting the knob to 135 microns and crushing, it was set to 10 microns and further pulverized. It was set to 4.
Comparative sample No. 3 had a β-1,3-1,4-glucan content of 6.1% by mass and an arabinoxylan content of 4.1% by mass. The composition ratio of arabinose and xylose constituting arabinoxylan was arabinose: xylose = 1: 1.5.
Comparative sample No. 4 had a β-1,3-1,4-glucan content of 1.6 mass% and an arabinoxylan content of 4.5 mass%. The composition ratio of arabinose and xylose constituting arabinoxylan was arabinose: xylose = 1: 1.8.

[Comparative Example 4]
Commercially available β-glucan (trade name: BARLEY BETA-GLUCAN, manufactured by Megazyme, containing 97% by mass of β-1,3-1,4-glucan, arabinoxylan undetected) was compared with Comparative Sample No. It was set to 5.

[Comparative Example 5]
A commercially available arabinoxylan (trade name: Arabinoxylan Rye, manufactured by Megazyme, containing 92.6% by mass of arabinoxylan, non-detected β-glucan) was compared with Comparative Sample No. It was set to 6.

[Examples 3 to 4 and Comparative Examples 6 to 11]
To 285 g of commercially available wheat flour (trade name: Quinn, manufactured by Nippon Flour Mill, Lot. T / 050912T445), sample No. 1-2, comparative sample no. 15 g of each of 1 to 6 was added and mixed, and then 130 g of 2% by weight saline was added and kneaded well to obtain raw noodles (formed into a shape having a thickness of 3 mm, a width of 5 mm, and a length of 20 cm). The raw noodles were boiled in boiling water for 12 minutes and cooled with running water for 30 seconds to obtain noodles.
The elasticity of noodles affects the texture. If the elasticity is too weak, it will not feel crunchy, and if it is too strong, it will not chew. The noodle texture is required to have an appropriate elasticity. In the present invention, the elasticity was evaluated by measuring energy required for breaking noodles, that is, breaking stress. If the elasticity is weak, the breaking stress is low, and if the elasticity is high, the breaking stress is high.
The noodles obtained above were allowed to stand for 20 minutes, and the breaking stress was measured using a creep meter (RE-3305 type, manufactured by Yamaden Co., Ltd.). The plunger is no. 49 (tip 1 mm width flat finish), noodles cut to a length of 5 cm were placed on a sample table and fixed with a texture sample holder φ40. The breaking stress was measured at a storage pitch of 0.05 sec, a measurement strain rate of 99%, and a measurement speed of 0.5 mm / sec.
The elasticity was evaluated according to the following criteria. The results are shown in Table 1.
◎: less than 15000N / m ² or more ^{17000N / m 2 ○: 13000N /} m 2 or more 15000N / m less than ² △: 11000N / m ² or more 13000N / m ² less ×: less than 9000N / m ² or more 11000N / m ² ×× : Less than 9000 N / m ²

Claims (2)

Step A for pulverizing barley so that particles having a particle size of 500 μm or more are 10% by volume or less and particles having a particle size of 40 μm or less are 30 to 95% by volume, and a particle size of 50 from the pulverized product obtained in Step A A composition obtained by a production method having a step B in which particles of ˜500 μm obtain a fraction of 80% by volume or more, wherein β-1,3-1,4-glucan is 9-99.9 mass % And arabinoxylan 0.1 to 50% by mass.   The composition according to claim 1, wherein the composition ratio of arabinose and xylose constituting arabinoxylan is arabinose: xylose = 1: 0.5 to 1: 5 (molar ratio).