JP4962575B2 - Reaction vessel - Google Patents

Description

  The present invention relates to a reaction vessel suitable for performing various analyzes and analyzes in the field of medical analysis and chemistry in the fields of biological analysis, biochemical analysis, or chemical analysis in general, and reaction processing for processing the reaction vessel. It is about the method.

A micro multi-chamber apparatus is used as a small reaction apparatus used for biochemical analysis or normal chemical analysis. As such an apparatus, for example, a microwell reaction vessel such as a microtiter plate in which a plurality of wells are formed on a flat substrate surface is used (see, for example, Patent Document 1).
Moreover, as a trace liquid weighing structure capable of quantitatively handling a trace amount of liquid, a first channel and a second channel, a third channel opening in the channel wall of the first channel, A structure having a fourth channel having a property of opening the channel wall of the two channels and connecting one end of the third channel and the second channel so that capillary attraction is relatively difficult to work than the third channel. Some are provided (see, for example, Patent Documents 2 and 3). According to the trace liquid weighing structure, after the liquid introduced into the first flow path is drawn into the third flow path, the liquid remaining in the first flow path is removed, and the volume of the third flow path is increased. A corresponding volume of liquid can be weighed into the second flow path.
JP-A-2005-177749 JP 2004-163104 A JP 2005-114430 A Japanese Patent No. 3454717

When a conventional microwell reaction vessel is used, the upper surface of the reaction vessel is open to the atmosphere. Therefore, foreign matter may enter the sample from the outside, and conversely, the reaction product may contaminate the external environment.
Further, in the trace liquid weighing structure disclosed in Patent Documents 2 and 3, liquid introduction ports are formed at both ends of the first flow path and at both ends of the second flow path. It is possible that the reaction products are open and pollute the external environment through these ports.
Therefore, an object of the present invention is to provide a reaction vessel that can prevent entry of foreign matters from the outside of the reaction vessel and environmental contamination to the outside, and a reaction processing method using the reaction vessel.

  A reaction container according to the present invention reacts a reaction plate having a plurality of reaction chambers for causing a sample to react, an elastic plate placed on the reaction plate so as to close an opening of the reaction chamber, and an elastic plate. And a holding plate disposed on the elastic plate for fixing to the plate.

  The elastic plate is provided with a flow path for supplying a solution containing a sample to the reaction chamber on the surface facing the reaction plate. The flow path includes a main flow path common to all reaction chambers and individual flow paths that branch from the main flow path and connect to the reaction chambers. Each individual channel includes a metering channel having a constant capacity and an injection channel provided between the metering channel and the reaction chamber.

  It is necessary to maintain a close contact state between the elastic plate and the reaction plate while maintaining liquid tightness. Therefore, an elastic material such as silicone resin or PDMS (polydimethylsiloxane) is used as the elastic plate. By using such an elastic material, the elastic plate can be kept in close contact with the reaction plate by self-adsorption. However, as time passes, air enters the boundary between the elastic plate and the reaction plate, so that it becomes impossible to maintain liquid tightness or air tightness. If even a slight liquid leakage occurs, it may not be possible to send the liquid to the measuring channel. In addition, if air leaks at a part of the boundary between the elastic plate and the reaction plate, the pressure can be uniformly applied to all the injection channels when the liquid is injected into the plurality of reaction chambers by the pressurized air. The liquid cannot be injected into the reaction chamber.

Therefore, in the present invention, a fixing mechanism is provided between the reaction plate, the elastic body plate, and the holding plate so as to fix the elastic body plate to the reaction plate by the holding plate while maintaining liquid tightness.
In the first form of such a fixing mechanism, the holding plate is provided with a plurality of protrusions for retaining on the surface on the reaction plate side. The elastic plate is provided with a through hole through which the protrusion is passed, and the reaction plate is provided with a hole into which the protrusion is inserted. The protrusions and the holes are positioned and dimensioned so that the holding plate is positioned with respect to the reaction plate and the elastic plate is fixed with respect to the reaction plate by the holding plate in a liquid-tight manner.

  In this way, the elastic plate is fixed to the reaction plate by fixing the holding plate to the reaction plate with the protrusion for preventing the drop, and the liquid tightness between the elastic plate and the reaction plate is maintained.

  In the second form of such a fixing mechanism, the surface of the holding plate on the reaction plate side protrudes in one direction from the tip portion at least in the outer peripheral region corresponding to the outer side of the region where each flow path is formed. A plurality of fixing protrusions having the same claw portion and made of the same material as the holding plate are provided. The elastic plate is provided with a through hole for passing the fixing protrusion at a position corresponding to the fixing protrusion. The reaction plate is provided with a hole in which the fixing protrusion is inserted and engaged with the claw portion at a position corresponding to the fixing protrusion. The dimensions of the fixing projection and the hole of the reaction plate are set so that the elastic plate is fixed to the reaction plate in a liquid-tight manner by the holding plate.

  As described above, a plurality of fixing protrusions are provided in the outer peripheral region corresponding to the outer side of the region where each flow path is formed in at least the elastic plate on the reaction plate side surface of the holding plate, and these fixing protrusions are used. By fixing the holding plate to the reaction plate, it is possible to keep both plates uniformly in close contact with each other while positioning the elastic body plate with respect to the reaction plate, thereby maintaining liquid tightness between the two plates.

  The fixing protrusion is made of the same material as the holding plate, that is, formed by integral molding with the holding plate. The fixing protrusion itself can be formed separately from the holding plate, but the step of mounting the fixing protrusion on the holding portion plate can be omitted by forming the fixing protrusion and the holding plate by integral molding. And cost reduction can be achieved.

  In order to position the elastic body plate accurately with respect to the reaction plate and press it against the reaction plate to obtain high liquid tightness, the fixing protrusion is located in the area inside the outer peripheral area of the reaction plate side surface of the holding plate. It is preferable that they are provided densely. However, since it is necessary to use a slide mold when molding a fixing protrusion having a claw protruding in one direction, it is necessary to increase the distance between the protrusions, and a plurality of fixing protrusions must be arranged closely. I can't. Therefore, there is a limit to the sealing performance between the elastic plate and the reaction plate.

  Therefore, the fixing protrusions are provided only in the outer peripheral region, and the holding plate has the same surface as that of the holding plate that does not have a claw at the tip in the region inside the outer peripheral region on the reaction plate side surface. It is preferable that a sealing protrusion made of a material is provided. The elastic plate is provided with a through-hole for passing the sealing protrusion at a position corresponding to the sealing protrusion, and the reaction plate has an inner diameter equal to the outer diameter of the fixing protrusion at a position corresponding to the fixing protrusion. It is preferable that a hole is provided.

  Since the sealing projection having no claw at the tip can be molded without using a slide mold, it can be densely arranged in a region inside the outer peripheral region of the reaction plate side surface of the holding plate. Sealing performance between the elastic plate and the reaction plate can be enhanced by densely arranging the fitting straight type sealing protrusions in a region inside the outer peripheral region.

  Here, the inner diameter of the hole in the reaction plate is “same diameter” as the outer diameter of the sealing protrusion, only when the inner diameter of the hole is exactly the same diameter as the outer diameter of the sealing protrusion. In addition, the case where the inner diameter of the hole is slightly smaller than the outer diameter of the fixing protrusion is included so that the sealing protrusion can be inserted by elastic deformation and the inserted sealing protrusion can be held by the elastic force.

  Furthermore, the positioning between the flow path formed in the elastic plate and the reaction chamber formed in the reaction plate needs to be accurately performed. If there is a deviation in this positioning, liquid leakage or air Or leak. This misalignment brings the same result as the deterioration of liquid and air tightness between the elastic plate and the reaction plate.

  Therefore, in a more preferable embodiment, in order to prevent this displacement of positioning, the elastic plate is formed with a concave or convex portion for positioning on the surface facing the holding plate, and the holding plate is formed on the surface facing the elastic plate. Positioning convex portions or concave portions that are fitted into the concave portions or convex portions are formed at positions corresponding to the concave portions or convex portions of the elastic plate. As a result, the elastic plate is positioned with respect to the holding plate, and the holding plate is positioned with respect to the reaction plate via the protrusion, whereby the flow path formed in the elastic plate is formed in the reaction chamber formed in the reaction plate. Positioned against.

  In another preferred embodiment, the injection channel is narrower than the metering channel, and the liquid is introduced at the liquid introduction pressure when the liquid is introduced into the main channel and the metering channel, and at the purge pressure when the liquid in the main channel is purged. It is set to a size having a flow path resistance that allows liquid to pass through in a pressurized state without passing through. In this case, since the liquid is injected into the reaction chamber by pressurization using the flow path resistance of the injection flow path, it is called a passive valve. In the passive valve, it is more important to maintain a close contact state between the elastic plate and the reaction plate while maintaining liquid tightness.

The contact angle of the injection channel with respect to the water droplets can be 90 degrees or more, and the area of the boundary between the injection channel and the metering channel can be 1 to 10000000 μm ² . Here, in the case where the injection channel is constituted by a plurality of channels, the above-mentioned area means the area of the boundary with each of the plurality of channels constituting the injection channel. When such a condition is satisfied, it is difficult for the liquid to enter the injection channel when the liquid is introduced into the main channel and the metering channel, and when the liquid is introduced into the main channel and the metering channel. The introduction pressure can be increased.

  The elastic plate preferably further includes a reaction chamber air vent channel connected to the reaction chamber. In that case, when liquid is injected into the reaction chamber from the injection flow path, air corresponding to the amount of the injected liquid can be discharged from the reaction chamber, so that liquid injection into the reaction chamber is facilitated.

  The main flow path, the individual flow path, and the reaction chamber air vent flow path are preferably closed as a whole. In this case, foreign substances can be prevented from entering the sample from the outside, and reaction products in the reaction container can also be prevented from contaminating the external environment.

  The main channel is formed with a narrowed channel downstream of the branch to each individual channel, and the channel resistance of the narrow channel is greater than the channel resistance of the metering channel. In addition, it is preferably set to be smaller than the channel resistance of the injection channel. In that case, when the liquid is supplied to the main flow path, the liquid is sequentially supplied from the upstream measurement flow path in accordance with the flow path resistance. That is, the liquid does not flow downstream through the main flow path until the liquid is fed to the most upstream side flow path and filled with the liquid. When the metering flow path is filled with liquid, the flow resistance of the main flow path at the branching position with respect to the measurement flow path is smaller than the flow resistance of the injection flow path. It is supplied to the downstream metering channel. In this manner, the liquid is sequentially filled up to the most downstream metering channel. Thereafter, the liquid remaining in the main flow path is purged with pressurized air, and if the entire main flow path is pressurized with air pressure, the liquid filled in each metering flow path is simultaneously injected into each reaction chamber.

  It is preferable that the reaction plate further includes a sample introduction part that can inject a sample from the outside through a sample introduction port provided in a hermetically sealed manner and is connected to the main flow path. The sample introduction port is preferably sealed by an elastic member that can be penetrated by a sharp dispensing instrument having a sharp tip and that can close the through hole by elasticity when the dispensing instrument is pulled out after penetration. Thereby, a sample liquid can be inject | poured into a sample introduction part via an elastic member, and it can prevent that a sample liquid leaks out of a sample introduction part after that.

  The sample introduction part forms a container, and the sample pretreatment liquid or reagent may be sealed in the container in advance. In that case, there is no need to dispense the sample pretreatment liquid or reagent into the sample introduction part.

  The reaction plate may further include a reagent container, which contains a reagent used for the reaction of the sample solution in advance and is sealed with a film, or has a cap that can be opened and closed to inject the reagent. Can be able to. In that case, it is not necessary to separately prepare a container for containing the reagent.

  The reaction plate can also be equipped with a gene amplification container for performing a gene amplification reaction. In that case, even in a sample solution containing only a very small amount of the gene to be measured, the analysis accuracy can be increased by amplifying the gene on the reaction vessel by a gene amplification reaction such as PCR method or LAMP method. And even if a gene amplification container is provided, if the gene amplification reaction is performed in a closed space and the analysis can be performed with the space closed after the analysis is completed, contamination from outside can be prevented. While being able to prevent contamination of other samples.

  The reaction vessel may further include a cylinder and a syringe including a plunger disposed in the cylinder. It is preferable to further include a switching valve for switching and connecting the syringe to the main channel and other channels. An example of the switching valve is a rotary valve.

  The reaction chamber is preferably made of a light transmissive material so that optical measurement can be performed from the bottom. In that case, the liquid in the reaction chamber can be measured optically without being moved to another container.

  In addition, if the reaction chamber contains a gene to be detected in the liquid introduced into the reaction chamber flow path, a base corresponding to the probe in the reaction chamber is provided. Detection of a gene having a sequence can be performed.

  In the reaction container according to the present invention, the elastic plate is fixed to the reaction plate by fixing the holding plate to the reaction plate with the protrusion of the retaining protrusion or the fixing protrusion. The liquid tightness between the reaction plates can be maintained. As a result, there is no inconvenience that the liquid cannot be sent to the measuring channel due to liquid leakage. In addition, when injecting liquids into multiple reaction chambers, it is possible to apply pressure uniformly to all the injection channels, and it is possible to inject liquids into all reaction chambers at the same time, resulting in analysis accuracy. Will improve.

  In addition, the work of fixing the holding plate to the reaction plate with the retaining protrusion or the fixing protrusion can be simply performed, so that adjustment such as alignment is not required, and the workability of the reaction container assembly work is improved. .

  Further, the elastic plate is positioned with respect to the holding plate, and the holding plate is positioned with respect to the reaction plate through the protrusion, so that the flow path formed in the elastic plate is in the reaction chamber formed in the reaction plate. Position.

FIG. 1A is a schematic plan view showing one embodiment of a reaction vessel. FIG. 1B is a schematic cross-sectional view in which a cross-section of the bellows, the drain space, the metering flow channel, the injection flow channel, and the reaction chamber air vent flow channel is added to the cross-sectional view at the AA position in FIG. 1A. FIG. 1C is a schematic cross-sectional view showing the vicinity of the syringe 51 and the bellows 53 in an enlarged manner. FIG. 1D is a cross-sectional view in the vicinity of a retaining protrusion for fixing the holding plate to the reaction plate, and is a cross-sectional view taken along the line XX in FIG. 1A. FIG. 2A is a schematic plan view showing another embodiment of the reaction vessel. FIG. 2B is a schematic cross-sectional view in which cross sections of the bellows, the drain space, the metering flow path, the injection flow path, and the reaction chamber air vent flow path are added to the cross-sectional view at the AA position in FIG. 2A. FIG. 2C is a schematic cross-sectional view showing the vicinity of the syringe 51 and the bellows 53 in an enlarged manner. FIG. 2D is a cross-sectional view in the vicinity of a retaining protrusion for fixing the holding plate to the reaction plate, and is a cross-sectional view at the position of line XX in FIG. 1A. FIG. 3 is an exploded sectional view showing the embodiment of FIGS. 1A and A2 and a schematic exploded perspective view of the switching valve. FIG. 4A is a schematic plan view showing the vicinity of one reaction chamber in the embodiment of FIGS. 1A and A2. FIG. 4B is a schematic perspective view showing the vicinity of the reaction chamber. FIG. 4C is a schematic cross-sectional view showing the vicinity of the reaction chamber. FIG. 5A is an enlarged plan view showing the sample container of the embodiment of FIGS. 1A and A2. 5B is a cross-sectional view taken along the line BB in FIG. 5A. FIG. 6A is an enlarged plan view showing the reagent container of the embodiment of FIGS. 1A and A2. 6B is a cross-sectional view taken along the line CC in FIG. 6A. FIG. 7A is an enlarged plan view showing the air suction container of the embodiment of FIGS. 1A and A2. 7B is a cross-sectional view taken along the line DD in FIG. 7A. FIG. 8 is a schematic cross-sectional view showing a reaction processing apparatus for processing the reaction container together with the reaction container. FIG. 9 is a plan view for explaining the operation of introducing the sample liquid from the sample container into the reaction chamber. FIG. 10 is a plan view for explaining the operation following FIG. FIG. 11 is a plan view for explaining the operation following FIG. FIG. 12 is a plan view for explaining the operation following FIG. FIG. 13 is a plan view for explaining the operation following FIG. FIG. 14 is a plan view for explaining the operation following FIG. FIG. 15 is a plan view for explaining the operation following FIG. FIG. 16 is an enlarged schematic cross-sectional view showing the vicinity of the reaction chamber of still another embodiment of the reaction vessel. FIG. 17 is an enlarged schematic cross-sectional view showing the vicinity of the reaction chamber of another embodiment of the reaction vessel. FIG. 18 is a schematic cross-sectional view showing, in an enlarged manner, the vicinity of the reaction chamber of another embodiment of the reaction vessel. FIG. 19A is a schematic plan view showing still another embodiment of the reaction vessel. FIG. 19B is a cross-sectional view taken along the line AA in FIG. 19A and shows cross sections of the metering channel 15, the injection channel 17, the reaction chamber air vent channels 19, 21, the liquid drain space 29, the air drain space 31, and the bellows 53. It is the added schematic sectional drawing. FIG. 19C is a schematic cross-sectional view showing the vicinity of the syringe 51 and the bellows 53 in an enlarged manner. 20A is a schematic exploded view of the switching valve of the embodiment of FIGS. 1A and A2, and shows a plan view and a cross-sectional view of the seal plate. FIG. 20B is a plan view and a cross-sectional view of the rotor upper of the same embodiment. FIG. 20C is a plan view and a cross-sectional view of the rotor base of this embodiment.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Reaction container 3 Reaction plate 5 Reaction chamber 11 Elastic body plate 13 Main flow path 15 Metering flow path 17 Injection flow path 19, 21 Reaction chamber air vent flow path 35 Sample container 35b, 35d, 35e Sample container air vent flow path 37 Reagent container 37b, 37d, 37e Reagent container air vent channel 39 Air suction container 39b, 39d, 39e Air suction container air vent channel 51, 87 Syringe 51a, 87a Cylinder 51b, 87b Plunger 51d, 87d Cover body 51e, 87e Sealing Stop space 53 Bellows (capacity variable part)
53c Syringe air vent channel 63, 95 Switching valve 73 Channel spacer 75 Protrusion 77 Injection channel 79 Reaction chamber air vent channel

  1A to 1D are views showing an embodiment of a reaction vessel, FIG. 1A is a schematic plan view, and FIG. 1B is a metering channel 15 and an injection channel 17 in a cross section at the position AA in FIG. 1A. FIG. 1C is a schematic cross-sectional view in which the vicinity of the syringe 51 and the bellows 53 is enlarged. FIG. 1C is a schematic cross-sectional view of the reaction chamber air vent channels 19 and 21, the liquid drain space 29, the air drain space 31 and the bellows 53. FIG. 1D is a cross-sectional view in the vicinity of a protrusion for fixing and fixing as a fixing mechanism for fixing the holding plate to the reaction plate, and is a cross-sectional view at the position of line XX in FIG. 1A. FIG. 3 is an exploded sectional view showing this embodiment and a schematic exploded perspective view of the switching valve. 4A to 4C are schematic views showing the vicinity of one reaction chamber of this embodiment. FIG. 4A is a plan view, FIG. 4B is a perspective view, and FIG. 4C is a cross-sectional view. 5A and 5B are enlarged views of the sample container, FIG. 5A is a plan view, and FIG. 5B is a cross-sectional view at the BB position in FIG. 5A. 6A and 6B are enlarged views of the reagent container, FIG. 6A is a plan view, and FIG. 6B is a cross-sectional view taken along the line CC in FIG. 6A. 7A and 7B are enlarged views of the air suction container, FIG. 7A is a plan view, and FIG. 7B is a cross-sectional view taken along the line DD in FIG. 7A.

An example of the reaction vessel will be described with reference to FIGS. 1A to 7B.
The reaction vessel 1 includes a plurality of reaction chambers 5 having openings on one surface of the reaction plate 3. In this embodiment, 6 × 6 reaction chambers 5 are arranged in a staggered manner. A reagent 7 and wax 9 are accommodated in the reaction chamber 5.

  The material of the reaction plate 3 including the reaction chamber 5 is not particularly limited. However, when the reaction vessel 1 is used as disposable, it is preferable that there is a material available at low cost. As such a material, for example, a resin material such as polypropylene and polycarbonate is preferable. When the substance in the reaction chamber 5 is detected by absorbance, fluorescence, chemiluminescence, bioluminescence, or the like, it should be formed of a light transmissive resin so that optical detection can be performed from the bottom side. Is preferred. Particularly when fluorescence detection is performed, the reaction plate 3 is made of a material such as a resin having a low autofluorescence property (a property of generating less fluorescence from itself) and a light transmitting resin such as polycarbonate. Is preferred. The thickness of the reaction plate 3 is 0.2 to 4.0 mm (millimeters), preferably 1.0 to 2.0 mm. From the viewpoint of low autofluorescence for fluorescence detection, the reaction plate 3 is preferably thin.

  Referring to FIGS. 1A to 1D and FIGS. 4A to 4C, the elastic plate 11 is disposed on the reaction plate 3 so as to cover the arrangement region of the reaction chambers 5. The elastic plate 11 is made of, for example, PDMS (polydimethylsiloxane) or silicone rubber. The thickness of the elastic plate 11 is, for example, 1.0 to 5.0 mm. The elastic plate 11 is provided with a groove on the joint surface with the reaction plate 3. The groove and the surface of the reaction plate 3 form a main channel 13, a metering channel 15, an injection channel 17, reaction chamber air vent channels 19 and 21, and drain space air vent channels 23 and 25. The main flow path 13, the metering flow path 15 and the injection flow path 17 constitute a reaction chamber flow path. A concave portion 27 disposed on the reaction chamber 5 is also formed on the joint surface of the elastic plate 11 with the reaction plate 3. In FIG. 1A and FIGS. 4A and 4B, only the groove and the concave portion of the elastic plate 11 are illustrated.

  The main flow path 13 is composed of a single flow path and is formed to be bent so as to pass through the vicinity of all the reaction chambers 5. One end of the main flow path 13 is connected to a flow path 13 a formed of a through hole provided in the reaction plate 3. The flow path 13a is connected to a port of a switching valve 63 described later. The other end of the main channel 13 is connected to a liquid drain space 29 formed in the reaction plate 3. The dimensions of the grooves constituting the main flow path 13 are, for example, a depth of 400 μm (micrometer) and a width of 500 μm. In addition, the main flow path 13 has a predetermined length downstream of the position where the measurement flow path 15 is connected, for example, a 250 μm portion is formed to be narrower than other portions, for example, the width is 250 μm. It is.

  The metering channel 15 is branched from the main channel 13 and provided for each reaction chamber 5. The end of the metering channel 15 opposite to the main channel 13 is disposed in the vicinity of the reaction chamber 5. The depth of the groove constituting the measuring channel 15 is 400 μm, for example. The measuring channel 15 has an internal capacity of a predetermined capacity, for example, 2.5 μL (microliter). The width dimension of the part connected to the main flow path 13 of the measurement flow path 15 is larger than the narrow part of the main flow path 13 described above, for example, 500 μm. Thereby, the flow resistance of the main flow path 13 is larger than that of the measurement flow path 15 in the portion where the measurement flow path 15 is branched with respect to the liquid flowing from one end of the main flow path 13. The liquid flowing from one end of the main channel 13 first flows into the metering channel 15, and after the metering channel 15 is filled with the liquid, it flows downstream through the narrowed portion of the main channel 13. It has become.

An injection channel 17 is also provided for each reaction chamber 5. One end of the injection channel 17 is connected to the metering channel 15. The other end of the injection channel 17 is connected to a concave portion 27 disposed on the reaction chamber 5 and led to the reaction chamber 5. The injection channel 17 is formed with a dimension that maintains liquid tightness in the reaction chamber 5 in a state where there is no pressure difference between the reaction chamber 5 and the injection channel 17. In this embodiment, the injection channel 17 is composed of a plurality of grooves, and the dimensions of the grooves are, for example, a depth of 10 μm, a width of 20 μm, a pitch of 20 μm, and 13 grooves in a width region of 500 μm. Is formed. Here, the area of the boundary between the groove constituting the injection channel 17 and the metering channel 15, that is, the cross-sectional area of the groove constituting the injection channel 17 is 200 μm ² . The recess 27 has a depth of, for example, 400 μm, and the planar shape is a circle smaller than the reaction chamber 5.

  A reaction chamber air vent channel 19 is provided for each reaction chamber 5. One end of the reaction chamber air vent channel 19 is connected to a recess 27 disposed on the reaction chamber 5 at a position different from the injection channel 17 and disposed on the reaction chamber 5. The reaction chamber air vent channel 19 is formed with a dimension that maintains liquid tightness in the reaction chamber 5 in a state where there is no pressure difference between the reaction chamber 5 and the reaction chamber air vent channel 19. The other end of the reaction chamber air vent channel 19 is connected to the reaction chamber air vent channel 21. In this embodiment, the reaction chamber air vent channel 19 is composed of a plurality of grooves, and the dimensions of the grooves are, for example, a depth of 10 μm, a width of 20 μm, a pitch of 20 μm, and 13 in a 500 μm width region. Grooves are formed.

  In this embodiment, a plurality of reaction chamber air vent channels 21 are provided. A plurality of reaction chamber air vent channels 19 are connected to each reaction chamber air vent channel 21. The reaction chamber air vent channel 21 is for connecting the reaction chamber air vent channel 19 to an air drain space 31 formed in the reaction plate 3. The dimensions of the grooves constituting the reaction chamber air vent channel 21 are, for example, a depth of 400 μm and a width of 500 μm.

  The drain space air vent channel 23 is for connecting the liquid drain space 29 to a port of a switching valve 63 described later. One end of the drain space air vent channel 23 is disposed on the liquid drain space 29. The other end of the drain space air vent channel 23 is connected to a channel 23 a formed of a through hole provided in the reaction plate 3. The flow path 23a is connected to a port of a switching valve 63 described later. The dimensions of the grooves constituting the drain space air vent channel 23 are, for example, a depth of 400 μm and a width of 500 μm.

  The drain space air vent channel 25 is for connecting the air drain space 31 to a port of a switching valve 63 described later. One end of the drain space air vent channel 25 is disposed on the air drain space 31. The other end of the drain space air vent channel 25 is connected to a channel 25 a formed of a through hole provided in the reaction plate 3. The flow path 25a is connected to a port of a switching valve 63 described later. The dimensions of the grooves constituting the drain space air vent channel 25 are, for example, a depth of 400 μm and a width of 500 μm.

  A holding plate 33 (not shown in FIG. 1A) is disposed on the elastic body plate 11. The holding plate 33 is for fixing the elastic body plate 11 to the reaction plate 3. A through hole is formed in the holding plate 33 at a position on the reaction chamber 5.

  A sample container 35, a reagent container 37, and an air suction container 39 are formed on the reaction plate 3 at positions different from the arrangement region of the reaction chamber 5 and the drain spaces 29 and 31. The sample container 35, the reagent container 37, and the air suction container 39 constitute a sealed container for the reaction container of the present invention.

  As shown in FIGS. 5A and 5B, a sample plate 35 a penetrating from the bottom to the back surface of the sample container 35 and a sample container air vent flowing from the front surface to the back surface are formed on the reaction plate 3 in the vicinity of the sample container 35. A path 35b is formed. A protrusion 35 c is arranged on the reaction plate 3 around the opening of the sample container 35. A sample container air vent channel 35d made of a through hole is formed in the protrusion 35c on the sample container air vent channel 35b. A sample container air vent channel 35e that connects the sample container 35 and the sample container air vent channel 35d is formed on the surface of the protrusion 35c.

  The sample container air vent channel 35e is formed by, for example, one or a plurality of pores having a width of 5 to 200 μm and a depth of 5 to 200 μm. In the sample container 35 and the sample container air vent channel 35d, This is for maintaining the liquid tightness of the sample container 35 in a state where there is no pressure difference. A septum 41, which is an elastic member, is formed on the projection 35c so as to cover the sample container 35 and the air vent channel 35d. The septum 41 is made of, for example, an elastic material such as silicone rubber or PDMS. The septum 41 can be penetrated by a dispensing device having a sharp tip, and the through-hole can be closed by elasticity when the dispensing device is pulled out after penetration. A septum stopper 43 for fixing the septum 41 is disposed on the septum 41. The septum stopper 43 has an opening on the sample container 35. In this embodiment, the reagent 45 is accommodated in the sample container 35 in advance.

  As shown in FIGS. 6A and 6B, the reaction plate 3 in the vicinity of the reagent container 37 includes a reagent channel 37a that penetrates from the bottom to the back of the reagent container 37 and a reagent container air vent channel that penetrates from the front to the back. 37b is formed. A protrusion 37 c is arranged on the reaction plate 3 around the opening of the reagent container 37. A reagent container air vent channel 37d made of a through hole is formed in the protrusion 37c on the reagent container air vent channel 37b. A reagent container air vent channel 37e that connects the reagent container 37 and the reagent container air vent channel 37d is formed on the surface of the protrusion 37c.

  The reagent container air vent channel 37e is formed by, for example, one or a plurality of pores having a width of 5 to 200 μm and a depth of 5 to 200 μm. In the reagent container 37 and the reagent container air vent channel 37d, This is for maintaining the liquid tightness of the reagent container 37 in a state where there is no pressure difference. A film 47 made of, for example, aluminum is formed on the protrusion 37c so as to cover the reagent container 37 and the air vent channel 37d. Dilution water 49 is accommodated in the reagent container 37.

  As shown in FIGS. 7A and 7B, the air suction container 39 has the same configuration as the reagent container 37. That is, the air suction channel 39a penetrating from the bottom of the air suction container 39 to the back surface and the air suction container air venting flow penetrating from the front surface to the back surface of the reaction plate 3 near the air suction container 39. A path 39b is formed. On the reaction plate 3 around the opening of the air suction container 39, a protrusion 39c provided with air suction containers air vent channels 39d and 39e is disposed. A film 47 made of, for example, aluminum is formed on the protrusion 39c. The air suction container 39 contains no liquid or solid but is filled with air.

A first example of a fixing mechanism for fixing the elastic body plate 11 to the reaction plate by the holding plate 33 will be described with reference to FIG. 1D.
The holding plate 33 is provided with a plurality of protrusions 100 for preventing it from coming off on the surface on the reaction plate 3 side. As shown in FIG. 1A, the protrusion 100 is provided at an appropriate position in the vicinity of the injection flow path 17 for each reaction chamber and around the region where the reaction chamber is disposed. The protrusion 100 has a base end portion 101 having a cylindrical shape and a spherical inflatable portion 102 at the tip. The protrusion 100 is provided with a notch 104 extending in the axial direction from the distal end portion to the base end portion 101, and is urged in the direction in which the notch 104 is opened by the elastic force of the protrusion 100 itself. In the state where the notch 104 is opened, the size of the inflating portion 102 is larger than the diameter of the base end portion 101. When a force in the direction of closing the notch 104 acts on the inflatable portion 102, the notch 104 can be closed, and when the notch 104 is closed, the size of the inflatable portion 102 becomes smaller than the diameter of the base end portion 101.

  The elastic body plate 11 has a circular through hole 110 through which the protrusion 100 is passed. The inner diameter of the through hole 110 is set to be equal to or larger than the diameter of the base end portion 101 of the protrusion 100.

  The reaction plate 3 has a hole 120 into which the protrusion 100 is inserted. The inner diameter of the hole 120 is set such that the holding plate 33 is positioned with respect to the reaction plate 3 through the protrusion 100 by contacting the base end portion 101 with the notch 104 of the protrusion 100 being opened.

  On the back side of the reaction plate 3, a flange 122 having a thickness increased for reinforcement is formed around the hole 120. The length of the base end portion 101 of the protrusion 100, that is, the length from the base end of the protrusion 100 to the portion where the inflating portion 102 has a larger dimension is the thickness of the elastic plate 11 and the reaction plate of the flange portion 122. It is set to be equal to or slightly shorter than the total thickness of three. Thereby, the elastic plate 11 is overlaid on the reaction plate 3, and the holding plate 33 is further overlaid thereon, and the elastic plate 11 is attached to the reaction plate 3 by the protrusion 100 of the holding plate 33 so as to be in the state of FIG. When fixed, the elastic plate 11 is fixed to the reaction plate 3 in a liquid-tight manner by the holding plate 33. In particular, when the length of the base end portion 101 of the protrusion 100 is set to be slightly shorter than the sum of the thickness of the elastic plate 11 and the thickness of the reaction plate 3 in the flange portion 122, the elastic body The plate 11 is fixed in a compressed state, and the elastic force of the elastic plate 11 works so that the elastic plate 11 is fixed to the reaction plate 3 more securely and securely. Become.

  When the protrusion 100 passes through the through hole 110 of the elastic plate 11 and the hole 120 of the reaction plate 3, the notch 104 is urged in the closing direction by the inner diameters of these holes, and the size of the expansion portion 102 is reduced. When the inflatable portion 102 passes through the hole, the notch 104 is opened by its own elastic force and the protrusion 100 is fixed in the hole 120.

  A second example of a fixing mechanism for fixing the elastic body plate 11 to the reaction plate by the holding plate 33 will be described with reference to FIGS. 2A to 2D. The structure other than the fixing mechanism is the same as that described with reference to FIGS. 3 to 7B for FIGS. 1A to 1D.

  A plurality of fixing protrusions 200 and sealing protrusions 202 are provided on the surface of the holding plate 33 on the reaction plate 3 side. The fixing protrusion 200 and the sealing protrusion 202 are formed by integral molding with the holding plate 33. Through holes 110 and 111 for passing these protrusions 200 and 202 are provided at positions corresponding to these protrusions 200 and 202 of the elastic plate 11, and correspond to these protrusions 200 and 202 of the reaction plate 3. Holes 120 and 121 into which these protrusions 200 and 202 are inserted are provided at positions.

  The fixing projections 200 are formed in a row only in a region (referred to as an outer peripheral region) that surrounds the outer side of the region where the flow paths 13, 17, 21, and 23 are formed. It is provided in the vicinity of each injection channel 17 in the inner region.

  A claw portion 200 a that protrudes outward from the surface of the holding plate 33 is provided at the distal end portion of the fixing projection 200. The through hole 110 of the elastic plate 11 has an inner diameter that allows the distal end portion of the fixing projection 200 having the claw portion 200 a to pass therethrough, while the inner diameter of the hole 120 of the reaction plate 3 is the same as the proximal end portion of the fixing projection 200. This is smaller than the tip of the fixing projection 200. Although not shown in the figure, a process is performed to temporarily increase the inner diameter of the hole 120 when the claw portion 200a passes, for example, a notch is formed in the periphery of the hole 120 of the reaction plate 3. Thus, the tip of the fixing projection 200 can be easily passed. The fixing protrusion 200 is engaged with the back surface side of the reaction plate 3 after the claw portion 200a passes through the hole 120 of the reaction plate 3 and is fixed in a state where the elastic plate 11 is sandwiched between the reaction plate 3 and the holding plate 33. The length dimension is set as follows.

  Since the plurality of fixing protrusions 200 are provided in the outer peripheral region, the holding plate 33 is uniformly fixed in a state where the elastic body plate 11 is pressed against the reaction plate 3 side. Further, the fixing protrusion 200 is inserted into the hole 120 of the reaction plate 3 through the through hole 110 of the elastic body plate 11, whereby the reaction plate 3 and the elastic body plate at the position where the fixing protrusion 200 is provided. 11 and the holding plate 33 are positioned.

  Here, the claw portion 200a protrudes in the outer direction of the surface of the holding plate 33, but is not limited thereto, and may protrude in the inner direction of the surface of the holding plate 33.

  The seal projection 202 is formed with a uniform thickness from the base end to the tip, and no claw is provided at the tip. The through hole 111 of the elastic plate 11 has an inner diameter that is the same as or slightly larger than the sealing protrusion 202. The inner diameter of the hole 121 of the reaction plate 3 is substantially the same as the outer diameter of the sealing projection 202. As a result, the sealing protrusion 202 inserted into the hole 121 is fixed in a state of being inserted into the hole 121.

  Since the projection such as the fixing projection 200 having the claw portion at the tip portion needs to use a slide type at the time of molding, it is necessary to increase the interval between the projections. Since the projection does not use a slide mold at the time of molding, the sealing projection 202 can be arranged densely.

  Such a plurality of sealing protrusions 202 are densely provided in the region where the flow paths 13, 17, 21, and 23 are formed, so that the adhesion between the elastic plate 11 and the reaction plate 3 is improved. And the liquid tightness between the plates 3 and 11 can be improved. Further, since the sealing projection 202 is inserted into the hole 121 through the through hole 101, the reaction plate 3, the elastic plate 11, and the holding plate 33 are positioned in the same manner as the fixing projection 200. Since the protrusions 202 are densely provided, the positioning accuracy of the reaction plate 3, the elastic body plate 11, and the holding plate 33 is also improved.

  Note that a fixing projection 200 may be provided in a region inside the outer peripheral region of the surface of the holding plate 33 on the reaction plate 3 side instead of the sealing projection 202 or together with the sealing projection 202.

  Thus, the holding plate 33 is positioned with respect to the reaction plate 3 by the protrusions 100, 200, and 202. On the other hand, the positioning between the elastic plate 11 and the reaction plate 3 is performed by positioning the elastic plate 11 with respect to the holding plate 33. In order to position the elastic plate 11 with respect to the holding plate 33, the elastic plate 11 is formed with a recess 112 at an appropriate position on the surface facing the holding plate 33. A convex portion 106 is formed at a position corresponding to the concave portion 112 of the elastic plate 11 on the opposite surface. By fitting the convex portion 106 into the concave portion 112, the elastic body plate 11 is positioned with respect to the holding plate 33, and the holding plate 33 is positioned and fixed to the reaction plate 3 through the protrusions 100, thereby elastic plate 11 is positioned with respect to the reaction chamber 5 formed in the reaction plate 3.

The reaction plate 3, the elastic plate 11, and the holding plate 33 can be manufactured by molding together with the protrusion 100, the concave portion 112, the convex portion 106, and the holes 110 and 120.
The protrusions 100, 200, and 202, the concave portion 112, the convex portion 106, and the holes 110 and 120 for fixing and positioning among the reaction plate 3, the elastic body plate 11, and the holding plate 33 are used in the description of the operation and other embodiments. Although illustration and description are omitted, the same one as shown in FIG. 1 is provided.

  1A to 1D and FIG. 3, the syringe 51 is provided on the surface of the reaction plate 3 at a position different from the arrangement region of the reaction chamber 5, the drain spaces 29 and 31, and the containers 35, 37, and 39. It has been. The syringe 51 is formed by a cylinder 51a formed on the reaction plate 3, a plunger 51b disposed in the cylinder 51a, and a cover body 51d. The reaction plate 3 is formed with a syringe flow path 51c penetrating from the discharge port provided at the bottom of the cylinder 51a to the back surface.

  The cover body 51d has flexibility in the sliding direction of the plunger 51b, and is connected to the cylinder 51a and the plunger 51b. The cover body 51d is for blocking the portion of the inner wall of the cylinder 51a that the plunger 51b contacts with the atmosphere outside the cylinder 51a while maintaining airtightness, and is surrounded by the cylinder 51a, the plunger 51b, and the cover body 51d. A sealing space 51e is formed. The end of the cover body 51d on the side connected to the cylinder 51a is fixed to the upper end of the cylinder 51a with a cylinder cap 51f while ensuring airtightness. The end of the cover body 51d on the side connected to the plunger 51b is connected to the upper surface of the plunger 51b with an adhesive while ensuring airtightness. However, the method and position of connecting the cover body 51d to the cylinder 51a and the plunger 51b are not limited to this.

  Thus, the cover body 51d is connected to the cylinder 51a and the plunger 51b to form a sealed space 51e surrounded by the cylinder 51a, the plunger 51b, and the cover body 51d, so that the space between the cylinder 51a and the plunger 51b is formed. Therefore, it is possible to prevent foreign substances from entering and environmental contamination of the liquid to the outside. Since the cover body 51d is flexible in the sliding direction of the plunger 51b, the sliding operation of the plunger 51b is possible.

  In this embodiment, the plunger 51b and the cover body 51d are formed by separate members, but the plunger and the cover body may be integrally formed. An example of the integrally formed plunger and cover material is silicone rubber.

  The reaction plate 3 is also provided with a bellows 53 at a position different from the arrangement region of the reaction chamber 5, the drain spaces 29 and 31, the containers 35, 37, and 39 and the syringe 51. The internal space of the bellows 53 is sealed, and the internal capacity is passively variable by expanding and contracting. For example, the bellows 53 is disposed in a through hole 53 a provided in the reaction plate 3.

  A container bottom 55 is attached to the back surface of the reaction plate 3 at a position different from the arrangement region of the reaction chamber 5. An air vent channel 53 b is provided in the container bottom 55 at a position communicating with the bellows 53. The bellows 53 is in close contact with the surface of the container bottom 55. The container bottom 55 is for guiding the flow paths 13a, 23a, 25a, 35a, 35b, 37a, 37b, 39a, 39b, 51c, 53b to a predetermined port position.

The reaction plate 3 container and the bottom 55 are provided with a syringe air vent channel 53 c having one end connected to the sealed space 51 e and the other end made a bellows 53. Illustration of the syringe air vent channel 53c in FIG. 1 (A) is omitted.
Thus, since the syringe air vent channel 53c having one end connected to the sealed space 51e and the other end bellows 53 is provided, the sealed space 51e is shielded from the atmosphere outside the reaction vessel 1, When the plunger 51b slides, the pressure change in the sealed space 51e accompanying the change in the internal capacity of the sealed space 51e can be reduced, and the plunger 51b can be slid smoothly.

  A rotary switching valve 63 including a disc-shaped seal plate 57, a rotor upper 59, and a rotor base 61 is provided on the surface of the container bottom 55 opposite to the reaction plate 3. The switching valve 63 is attached to the container bottom 55 by a lock 65.

  The seal plate 57 is provided in the vicinity of the peripheral edge thereof, and is connected to any of the flow paths 13a, 35a, 37a, 39a, and the flow paths 23a, 25a, 35b, A through groove 57b connected to at least two of 37b, 39b, and 53b and a through hole 57c provided in the center and connected to the syringe flow path 51c are provided.

  The rotor upper 59 includes a through hole 59a provided at the same position as the through hole 57a of the seal plate 57, a groove 59b provided on the surface corresponding to the through groove 57b of the seal plate 57, and a through hole provided in the center. A hole 59c is provided.

  The rotor base 61 is provided with a groove 61a on its surface for connecting two peripheral holes 59a and 59c disposed at the center and the peripheral portion of the rotor upper 59.

  The syringe channel 51c is connected to one of the channels 13a, 35a, 37a, 39a by the rotation of the switching valve 63, and at the same time, the air vent channel 53b is connected to the channels 23a, 25a, 35b, 37b, 39b. Connected to at least one of them.

  The position of the switching valve 63 shown in FIG. 1A is that the syringe flow path 51c is not connected to any of the flow paths 13a, 35a, 37a, 39a, and the air vent flow path 53b is also connected to the flow paths 23a, 25a, 35b, The position in the initial state where neither of 37b and 39b is connected is shown.

  In the reaction vessel 1, the injection channel 17 is formed so as to maintain the liquid tightness of the reaction chamber 5 with no pressure difference between the reaction chamber 5 and the injection channel 17. The reaction chamber air vent channel 19 is also formed so as to maintain the liquid tightness of the reaction chamber 5 in a state where there is no pressure difference between the reaction chamber 5 and the reaction chamber air vent channel 19. The main flow path 13 of the reaction chamber flow path, the liquid drain space 29 to which the main flow path 13 is connected, and the drain space air vent flow path 23 can be sealed by switching a switching valve 63. The containers 35, 37, and 39 are sealed with a septum 41 or a film 47. The flow paths 35 a, 35 b, 37 a, 37 b, 39 a, 39 b connected to the containers 35, 37, 39 can be sealed by switching the switching valve 63. One end of the air vent channel 53b is connected to the bellows 53 and sealed. Thus, the container and flow path inside the reaction container 1 are formed in a closed system. Even when the air vent channel 53 b is connected to the atmosphere outside the reaction vessel 1 without the bellows 53, the air vent channel 53 b is connected to the reaction vessel 1 by switching the switching valve 63. Since it can block | block from an internal container and flow paths other than the air vent flow path 53b, the container and flow path in which a liquid is accommodated or a liquid flows can be made into a closed system.

FIG. 8 is a cross-sectional view showing a reaction processing apparatus for processing the reaction vessel 1 together with the reaction vessel 1. Although the fixing mechanism is not shown in the reaction vessel 1, it includes both the reaction vessel 1 of FIGS. 1A to 1D and the reaction vessel 1 of FIGS.
The reaction processing apparatus includes a temperature adjustment mechanism 67 for adjusting the temperature of the reaction chamber 5, a syringe driving unit 69 for driving the syringe 51, and a switching valve driving unit 71 for switching the switching valve 63.

  9 to 15 are plan views for explaining the operation of introducing the sample liquid from the sample container 35 into the reaction chamber 5. This operation will be described with reference to FIGS. 1A to 1D and FIGS. The same applies when applied to the reaction vessels of FIGS.

  Using a dispensing device having a sharp point (not shown), for example, 5 μL of sample liquid is dispensed into the sample container 35 through the septum 41 on the sample container 35. After dispensing the sample solution, pull out the dispensing device. The through hole of the septum 41 when the dispensing instrument is pulled out is closed by the elasticity of the septum 41.

The syringe drive unit 69 is connected to the plunger 51 b of the syringe 51, and the switching valve drive unit 71 is connected to the switching valve 63.
As shown in FIG. 9, the switching valve 63 is rotated from the state of the switching valve 63 shown in FIG. 1A to connect the sample channel 35a and the syringe channel 51c, and the sample container air vent channel 35b is connected to the air vent channel. Connect to 53b. At this time, the air vent channels 37b and 39b are also connected to the air vent channel 53b. For example, 45 μL of the reagent 45 is accommodated in the sample container 35.

  The plunger 51b of the syringe 51 is slid to mix the sample liquid and the reagent 45 in the sample container 35. Thereafter, the mixed solution in the sample container 35 is sucked into the flow path in the switching valve 63, the syringe flow path 51c, and the syringe 51 by, for example, 10 μL. At this time, since the sample container 35 is connected to the bellows 53 via the air vent channels 35e, 35d, 35b, the switching valve 63 and the air vent channel 53b, the gas capacity in the sample container 35 is changed. The bellows 53 expands and contracts. Further, the cover body 51d is deformed by the sliding of the plunger 51b, and the internal capacity of the sealed space 51e (see FIG. 1C) is changed. Since the sealed space 51e is connected to the bellows 53 via the syringe air vent channel 53c, the bellows 53 expands and contracts even when the internal capacity of the sealed space 51e changes. Even in the operation process described below, the bellows 53 expands and contracts with the change in the internal capacity of the sealed space 51e due to the sliding of the plunger 51b.

  As shown in FIG. 10, the switching valve 63 is rotated to connect the reagent channel 37a and the syringe channel 51c, and the reagent container air vent channel 37b is connected to the air vent channel 53b. For example, 190 μL of dilution water 49 is accommodated in the reagent container 37. The mixed solution sucked into the flow path in the switching valve 63, the syringe flow path 51 c and the syringe 51 is injected into the reagent container 37, and the syringe 51 is slid to mix the mixed liquid and the dilution water 49. For example, all of the diluted mixed solution is sucked into the flow path in the switching valve 63, the syringe flow path 51c, and the syringe 51, that is, 200 μL. At this time, since the reagent container 37 is connected to the bellows 53 via the air vent channels 37e, 37d, 37b, the switching valve 63 and the air vent channel 53b, the gas capacity in the reagent container 37 is changed. The bellows 53 expands and contracts.

  As shown in FIG. 11, the switching valve 63 is rotated to connect the flow path 13 a connected to one end of the main flow path 13 and the syringe flow path 51 c, and the flow connected to the liquid drain space 29 and the air drain space 31. The paths 23a and 25a are connected to the air vent channel 53b. The syringe 51 is driven in the pushing direction to send the diluted mixed solution sucked into the flow path in the switching valve 63, the syringe flow path 51 c, and the syringe 51 to the main flow path 13. The diluted mixed liquid injected from the flow path 13a side into the main flow path 13 fills the metering flow path 15 in order from the flow path 13a side and reaches the liquid drain space 29 as indicated by the embossments and arrows. In the introduction pressure state when the diluted mixture is introduced into the main channel 13 and the metering channel 15, the injection channel 17 allows gas to pass but does not allow the diluted mixture to pass. The gas in the metering channel 15 moves into the reaction chamber 5 through the injection channel 17 as the metering channel 15 is filled with the diluted mixed solution. As this gas moves, a part of the gas in the reaction chamber 5 moves to the reaction chamber air vent channels 19 and 21. Further, the gas in the channel from the reaction chamber air vent channel 19 to the bellows 53 sequentially moves to the bellows 53 side (see white arrow). Further, when the diluted mixed liquid is injected into the liquid drain space 29, the gas in the flow path from the liquid drain space 29 to the bellows 53 sequentially moves toward the bellows 53 (see the white arrow). As a result, the bellows 53 expands.

  As shown in FIG. 12, the switching valve 63 is rotated to connect the air suction channel 39a and the syringe channel 51c, and the air suction container air vent channel 39b is connected to the air vent channel 53b. The syringe 51 is driven to the suction side, and the gas in the air suction container 39 is sucked into the flow path in the switching valve 63, the syringe flow path 51 c, and the syringe 51. At this time, since the air suction container 39 is connected to the bellows 53 via the air vent channels 39e, 39d, 39b, the switching valve 63 and the air vent channel 53b, the pressure inside the air suction container 39 is reduced. As a result, the bellows 53 contracts (see the white arrow).

  As shown in FIG. 13, the switching valve 63 is rotated to connect the flow path 13a and the syringe flow path 51c, and connect the flow paths 23a and 25a to the air vent flow path 53b as in the connection state of FIG. The syringe 51 is driven in the pushing direction, and the flow path in the switching valve 63, the syringe flow path 51c, and the gas in the syringe 51 are sent to the main flow path 13 to purge the diluted mixed liquid in the main flow path 13 (open arrow) reference). In the purge pressure state at this time, the dilute mixed liquid does not pass through the injection flow path 17, and therefore the dilute mixed liquid remains in the measuring flow path 15 (see embossing). The purged diluted liquid mixture is accommodated in the liquid drain space 29. Further, when the diluted mixed liquid is injected into the liquid drain space 29, the gas in the flow path from the liquid drain space 29 to the bellows 53 sequentially moves toward the bellows 53 (see the white arrow). As a result, the bellows 53 expands.

  As shown in FIG. 14, the switching valve 63 is rotated to connect the air suction flow path 39a and the syringe flow path 51c as in the connection state of FIG. 12, and the air suction container air vent flow path 39b is vented. Connect to the flow path 53b. The syringe 51 is driven to the suction side, and the gas in the air suction container 39 is sucked into the flow path in the switching valve 63, the syringe flow path 51 c, and the syringe 51. At this time, the bellows 53 contracts as described with reference to FIG. 12 (see the white arrow).

  As shown in FIG. 15, the switching valve 63 is rotated to connect the flow path 13a and the syringe flow path 51c, and connect the flow path 25a to the air vent flow path 53b. This connection state differs from the connection state shown in FIGS. 11 and 13 in that the liquid drain space 29 to which the downstream end of the main flow path 13 is connected is not connected to the flow path in the switching valve 63. The syringe 51 is driven in the pushing direction. Since the downstream end of the main flow path 13 is not connected to the bellows 53, the inside of the main flow path 13 is pressurized larger than the liquid introduction pressure and the purge introduction pressure. As a result, the diluted mixed solution in the metering channel 15 is injected into the reaction chamber 5 through the injection channel 17. After the diluted mixed solution is injected into the reaction chamber 5, a part of the gas in the main channel 13 flows into the reaction chamber 5 through the metering channel 15 and the injection channel 17. At this time, the reaction chamber 5 is connected to the bellows 53 via the reaction chamber air vent channels 19 and 21, the air drain space 31, the drain space air vent channel 25a, and the air vent channel 53b. The gas between the bellows 53 sequentially moves toward the bellows 53 (see the white arrow). As a result, the bellows 53 expands.

  After the switching valve 63 is connected as shown in FIG. 1 and the vessel, flow path and drain space inside the reaction vessel 1 are sealed, the reaction chamber 5 is heated by the temperature control mechanism 67 to melt the wax 9. Thereby, the diluted mixed solution injected into the reaction chamber 5 enters under the wax 9, and the diluted mixed solution and the reagent 7 are mixed and reacted. Thus, according to the reaction vessel 1, the reaction treatment can be performed in a closed system.

Before injecting the diluted mixed solution into the reaction chamber 5, the reaction chamber 5 is heated by the temperature adjustment mechanism 67 to melt the wax 9, and the wax 9 is injected when the diluted mixed solution is injected into the reaction chamber 5. May be melted. In this case, the diluted mixed solution injected into the reaction chamber 5 immediately enters under the wax 9, and the diluted mixed solution and the reagent 7 are mixed and reacted. Even if the connection state of the switching valve 63 is the state shown in FIG. 15, the sealed system is secured by the bellows 53. If the switching valve 63 is brought into the connection state shown in FIG. 1 after the diluted mixed solution is injected, the container, flow path and drain space inside the reaction container 1 can be sealed. Here, the timing of switching the switching valve 63 to the connected state of FIG. 1A may be any timing from immediately after the injection of the diluted mixed solution to the end of the reaction of the diluted mixed solution and the reagent 7, or the diluted mixed solution and the reagent 7. It may be after the completion of the reaction.
Thus, according to the reaction vessel 1, the reaction process can be performed in a closed system, and the closed system can be formed before and after the reaction process.

  In the embodiment of FIGS. 1A to 1D and FIGS. 2A to 2D, the grooves for forming the flow paths 13, 15, 17, 19, 21, 23 are formed in the elastic plate 11, but the present invention is not limited to this. However, a groove for forming all or part of the flow paths may be formed on the surface of the reaction plate 3.

  FIG. 16 is an enlarged schematic cross-sectional view showing the vicinity of the reaction chamber of another embodiment of the reaction vessel. This embodiment is the same as the above-described embodiment described with reference to FIGS. 1A to 1D to 15 except that a flow path spacer is disposed between the reaction chamber base and the elastic plate.

  A flow path spacer 73 is arranged on the reaction plate 3 so as to cover the arrangement region of the reaction chamber 5, and an elastic body plate 11 and a holding plate 33 are further arranged in that order. The channel spacer 73 is made of, for example, PDMS or silicone rubber. The thickness of the channel spacer 73 is, for example, 0.5 to 5.0 mm. The flow path spacer 73 includes a convex portion 75 protruding into the reaction chamber 5 for each reaction chamber 5. The convex portion 75 has a substantially trapezoidal cross section. For example, the base end has a width of 1.0 to 2.8 mm, the tip has a width of 0.2 to 0.5 mm, and the tip has a base end. It is thinner than Further, the surface of the convex portion 75 is subjected to super water repellent treatment. However, the surface of the convex portion 75 may not necessarily be subjected to the water repellent treatment.

Further, the flow path spacer 73 is provided with an injection flow path 77 formed of a through hole penetrating from the tip end portion of the convex portion 75 to the opposite surface for each position where the convex portion 75 is formed. The inner diameter of the injection channel 77 is, for example, 500 μm. The opening on the elastic plate 11 side of the injection channel 77 is connected to the injection channel 17 of the elastic plate 11. In this embodiment, the elastic plate 11 is not provided with the concave portion 27 as compared with the above-described embodiment described with reference to FIGS.
Further, the flow path spacer 73 is also provided with a reaction chamber air vent channel 79 including a through hole for communicating the reaction chamber air vent channel 19 of the elastic plate 11 and the reaction chamber 5.

  Although not shown, the channel spacer 73 includes both ends of the main channel 13, ends of the reaction chamber air vent channel 21 on the air drain space 31 side, and both ends of the drain space air vent channels 23 and 25. The part is provided with a through hole, and these flow paths 13, 21, 23, 25 are connected to containers 29, 31 or flow paths 23 a, 25 b provided in the reaction plate 3.

  In this embodiment, the end of the injection flow channel 77 opposite to the injection flow channel 15 (the other end of the injection flow channel) is disposed at the tip of a convex portion 75 formed to protrude from the inner upper surface of the reaction chamber 5. Therefore, the liquid injected into the reaction chamber 5 through the injection channels 15 and 77 can be easily dropped into the reaction chamber 5.

  Further, when the liquid is discharged from the tip of the convex portion 75 through the injection channel 77, the tip of the convex portion 75 is adjusted so that the droplet formed at the tip of the convex portion 75 contacts the side wall of the reaction chamber 5. If it is arranged near the side wall of the reaction chamber 5, the liquid can be injected into the reaction chamber 5 along the side wall of the reaction chamber 5, and the liquid can be injected into the reaction chamber 5 more reliably. However, the formation position of the convex portion 75 may be a position where a droplet formed at the tip of the convex portion 75 does not contact the side wall of the reaction chamber 5.

FIG. 17 is an enlarged schematic cross-sectional view showing the vicinity of the reaction chamber of another embodiment of the reaction vessel.
Compared with the embodiment described with reference to FIG. 16, this embodiment further includes a protrusion 81 inside the reaction chamber 5. The tip of the protrusion 81 is disposed below the tip of the protrusion 75. Thereby, it becomes easy to guide the droplet formed at the tip of the convex portion 75 into the reaction chamber 5. In particular, it is particularly effective if a hydrophilic treatment is applied to at least the tip surface of the protrusion 81.

FIG. 18 is a schematic cross-sectional view showing, in an enlarged manner, the vicinity of the reaction chamber of another embodiment of the reaction vessel.
In this embodiment, compared to the embodiment described with reference to FIG. 17, the stepped portion 83 formed on the side wall of the reaction chamber 5 and the upper surface of the reaction chamber 5 are formed on the upper surface of the stepped portion 83 with a gap. Further provided is a convex ridge 85. The step part 83 and the protruding line part 85 are formed in an annular shape when viewed from above. The tip of the ridge 85 is disposed at a distance from the side wall of the reaction chamber 5.

  Since the tip of the ridge 85 is spaced from the upper surface and the side surface of the reaction chamber 5, the liquid contained in the reaction chamber 5 reaches the upper surface of the reaction chamber 5 along the side wall of the reaction chamber 5. Can be prevented. This effect is particularly effective when a water-repellent treatment is performed on at least the tip of the ridge 85.

  The configuration provided with the step portion 83 and the convex strip portion 85 shown in FIG. 18 can also be applied to the embodiment shown in FIG.

  In each embodiment described with reference to FIG. 16, FIG. 17 or FIG. 18, grooves for forming the flow paths 13, 15, 17, 19, 21, 23 are formed in the elastic body plate 11. The present invention is not limited to this, and the grooves for forming all or part of the flow paths are the elastic plate 11 side surface of the flow path spacer 73 and the reaction plate 11 side surface of the flow path spacer 73. It may be formed on any surface of the reaction plate 3.

  Regarding the syringe 51, a part of the cylinder 51 a may be formed by a part of the switching valve 63.

  19A to 19C are views showing still another embodiment of the reaction vessel, FIG. 19A is a schematic plan view, and FIG. 19B is a cross-section at the position AA in FIG. 17, a schematic cross-sectional view of the reaction chamber air vent channels 19 and 21, the liquid drain space 29, the air drain space 31, and the bellows 53, and FIG. FIG. 20A to 20C are schematic exploded views of the switching valve, FIG. 20A is a plan view and a sectional view of a seal plate, FIG. 20B is a plan view and a sectional view of a rotor upper, and FIG. 20C is a plan view and a sectional view of a rotor base. The figure is shown.

In this embodiment, the cylinder 87 a of the syringe 87 is formed of a resin material such as polypropylene or polycarbonate, and is integrally formed with the rotor upper 91 of the switching valve 95.
The syringe 87 is formed by a cylinder 87a disposed in a through hole formed in the reaction plate 3 and the container bottom 55, a plunger 87b disposed in the cylinder 87a, and a cover body 87d.

  The cover body 87d has flexibility in the sliding direction of the plunger 87b, and is connected to the cylinder 87a and the plunger 87b. The cover body 87d is for shutting off the portion of the inner wall of the cylinder 87a that contacts the plunger 87b while maintaining airtightness from the atmosphere outside the cylinder 87a. The cover body 87d is surrounded by the cylinder 87a, the plunger 87b, and the cover body 87d. A sealing space 87e is formed.

  The end of the cover body 87d on the side connected to the cylinder 87a is fixed to the upper end of the cylinder 87a with a cylinder cap 87f while ensuring airtightness. Further, the end of the cover body 87d on the side connected to the plunger 87b is connected to the upper surface of the plunger 87b with an adhesive while ensuring airtightness. However, the method and position for connecting the cover body 87d to the cylinder 87a and the plunger 87b are not limited thereto. The plunger and the cover body may be integrally formed. An example of the integrally formed plunger and cover material is silicone rubber.

  As described above, the cover body 87d is connected to the cylinder 87a and the plunger 87b to form a sealed space 87e surrounded by the cylinder 87a, the plunger 87b, and the cover body 87d, so that the space between the cylinder 87a and the plunger 87b is formed. Therefore, it is possible to prevent foreign substances from entering and environmental contamination of the liquid to the outside. Since the cover body 87d has flexibility in the sliding direction of the plunger 87b, the sliding operation of the plunger 87b is possible.

  The syringe air vent channel 53c and the switching valve 95 will be described with reference to FIG.

  The switching valve 95 is formed by a disc-shaped seal plate 89, a rotor upper 91 and a rotor base 93. The switching valve 95 is attached to the container bottom 55 by a lock 65.

  The seal plate 89 is provided in the vicinity of the peripheral edge portion thereof, and is connected to any of the flow paths 13a, 35a, 37a, 39a, and the flow paths 23a, 25a, 35b, A through groove 89b connected to at least two of 37b, 39b, and 53b and a through hole 89c provided at the center and into which the cylinder 87a is inserted are provided. A fluororesin layer (not shown) is formed on the surface of the seal plate 89 facing the container bottom 55.

  The rotor upper 91 corresponds to a cylindrical cylinder 87 a provided at the center of one surface thereof, a through hole 91 a provided at the same position as the through hole 89 a of the seal plate 89, and a through groove 89 b of the seal plate 89. Then, a groove 91b provided on the surface, a through hole 91c provided in the groove 91b, and a through hole 91d provided in the center are provided. The through hole 91d is provided at the bottom of the cylinder 87a and constitutes a discharge port of the cylinder 87a.

  The rotor upper 91 is also formed with a syringe air vent channel 53c formed of a through hole penetrating from the upper end surface of the cylinder 87a to the back surface of the rotor upper 91. A cutout is formed in the upper end surface of the cylinder 87a from the inner wall of the cylinder 87a to the syringe air vent channel 53c. By this notch, as shown in FIG. 19C, the sealing space 87e and the syringe air vent channel 53c communicate with each other with the upper end surface of the cylinder 87a covered with the cover body 87d.

  The rotor base 93 has a groove 93a for connecting the through hole 91a and the through hole 91d formed in the rotor upper 91 on the surface to be bonded to the back surface of the rotor upper 91, and a syringe air vent formed in the rotor upper 91. A groove 93b for connecting the flow paths 53c and 91c is provided.

  As shown in FIG. 19B, the seal plate 89, the rotor upper 91, and the rotor base 93 are arranged so that a cylinder 87 a is inserted into the through hole 89 c of the seal plate 89 and overlapped to form a switching valve 95.

  The through hole 91d of the rotor upper 91 that constitutes the discharge port of the cylinder 87a is connected to the through hole 89a of the seal plate 89 through the groove 93a of the rotor base 93 and the through hole 91a of the rotor upper 91.

  The sealing space 87e (see FIG. 19C) is connected to the through groove 89b of the seal plate 89 via the syringe air vent channel 53c, the groove 93b of the rotor base 93, the through hole 91c and the through groove 91b of the rotor upper 91. The

The flow path connection will be described with reference to FIGS. 19A to 19C and FIG.
Through the rotation of the switching valve 95, the through hole 91d of the rotor upper 91 constituting the discharge port of the cylinder 87a is one of the flow paths 13a, 35a, 37a, 39a via the groove 93a, the through hole 91a, and the through hole 89a. Connected to.

  At the same time that the through hole 91d is connected to any of the flow paths 13a, 35a, 37a, 39a, the air vent flow path 53b is connected to the flow paths 23a, 25a, 35b, 37b, 39b via the through grooves 89b, 91b. Connected to at least one of them. At this time, the sealing space 87e is connected to the air vent channel 53b via the cylinder air vent channel 53c, the groove 93b, the through hole 91c, and the through grooves 89b and 91b.

  According to this embodiment, the flow path between the syringe 87 and the switching valve 95 can be eliminated, and the flow path configuration is simplified.

  When there is a seam in the flow path, leakage of liquid or gas may occur at the seam part, or a liquid pool may occur at the seam part. In this embodiment, since the cylinder 87a and the rotor upper 91 are integrally formed, there is no joint between the flow path between the syringe 87 and the switching valve 95, and leakage and liquid pool between the syringe 87 and the switching valve 95 are generated. Can be eliminated.

  If a liquid puddle occurs at the joint, there are concerns about the volume of the liquid being pumped down, liquid carryover or contamination due to mixing of the liquid in the puddle and other liquids being pumped, and concerns about concentration fluctuations. However, in this embodiment, these concerns between the syringe 87 and the switching valve 95 can be eliminated.

  When the syringe is arranged on the switching valve, if the flow path 51c is formed between the syringe 51 and the switching valve 63 as shown in FIG. 1B, the cylinder 51a is placed in the portion where the flow path 51c is formed. 19A to 19C, there is no flow path between the syringe 87 and the switching valve 95. Therefore, even if the cylinders 51a and 81a have the same plane size, the capacity of the cylinder 87a is reduced. It can be made larger than the cylinder 51a.

  When the cylinder 87a is formed with the same capacity as the cylinder 51a, the height of the cylinder 87a is lower than the cylinder 51a with the same plane size as the cylinder 51a, or the cylinder 51a has the same plane size as the cylinder 51a. Or smaller.

  For example, even if the upper end surface of the cylinder 51a has to be disposed so as to protrude from the upper surface of the entire reaction vessel 1 due to the limitation of the planar size of the entire reaction vessel 1, the height of the cylinder 87a is the same as that of the cylinder 51a. Since the size can be made lower than that of the cylinder 51a, the upper end surface of the cylinder 87a can be arranged at the same position as or lower than the upper surface of the entire reaction vessel 1. This eliminates problems when the upper end surface of the cylinder protrudes from the upper surface of the entire reaction container, such as problems when stacking and storing a plurality of reaction containers and problems such as an increase in the packaging of the reaction container. it can.

  If the plane size of the cylinder 87a is made smaller than that of the cylinder 51a with the same capacity as the cylinder 51a, the plane size of the entire reaction vessel 1 can be reduced.

  The embodiments of the present invention have been described above, but the present invention is not limited to these, and the shape, material, arrangement, number, dimensions, flow path configuration, etc. are examples, and are described in the claims. Various modifications are possible within the scope of the present invention.

  For example, the bellows 53 connected to the air vent channel 53b may have another structure as long as the capacity is a variable capacity member whose internal capacity is passively variable. Examples of such a structure include a bag-like material made of a flexible material and a syringe-like material.

  The capacity variable member such as the bellows 53 is not necessarily provided.

  If liquids such as reagents are not previously stored in the containers 35, 37, and 39, it is not always necessary to provide the channels 35 e, 37 e, and 39 e made of pores in a part of the air vent channel.

  In the above embodiment, the air vent channels 35b, 37b, 39b provided in communication with the containers 35, 37, 39 as sealing containers are connected to the air vent channel 53b via the switching valve 63. The air vent channel provided in communication with the sealing container may be directly connected to the outside of the reaction container or a capacity variable part such as the bellows 53.

  An openable / closable cap may be used as a sealing method for the containers 35, 37, and 39.

  In the above embodiment, the reaction plate 3 is formed by one component, but the reaction plate may be formed by a plurality of components.

  The reagent in the reaction chamber 5 may be a dry reagent.

  The reagent may not be stored in advance in the sample container 35 or the reaction chamber 5.

  In the above embodiment, the dilution water 49 is stored in the reagent container 37, but the reagent may be stored instead of the dilution water 49.

  You may make it equip the reaction plate 3 with the gene amplification container for performing gene amplification reaction. For example, if the reagent container 37 is emptied, it can be used as a gene amplification container.

  If a reagent for performing a gene amplification reaction is accommodated in the reaction chamber 5, the gene amplification reaction can be performed in the reaction chamber 5.

  When a gene is contained in the liquid introduced into the main flow path 13, a probe that reacts with the gene may be provided in the reaction chamber 5.

  In the said Example, although the syringe 51 is arrange | positioned on the switching valve 63, the position which arrange | positions the syringe 51 is not limited on the switching valve 63, and may be anywhere.

  In the above embodiment, the rotary type switching valve 63 is used as the switching valve, but the switching valve is not limited to this, and various flow path switching valves can be used. A plurality of switching valves may be provided.

  In the above embodiment, when the liquid filled in the metering channel 15 is injected into the reaction chamber 5 through the injection channel 17, the liquid is injected into the reaction chamber 5 by pressurizing the main channel 13 after air purge. However, the reaction treatment method of the present invention is not limited to this. For example, by changing the flow path configuration so that the inside of the reaction chamber air vent channel 21 can be set to a negative pressure using the syringe 51, and by setting the inside of the reaction chamber air vent channel 21 and thus the inside of the reaction chamber 5 to a negative pressure. The liquid filled in the metering channel 15 may be injected into the reaction chamber 5 through the injection channel 17. Alternatively, a separate syringe may be prepared so that the liquid is injected into the reaction chamber 5 with a positive pressure in the main channel 13 and a negative pressure in the reaction chamber 5.

  In the above embodiment, one main flow path 13 is provided, and all the metering flow paths 15 are connected to the main flow path 13, but the flow path configuration is not limited to this. For example, a plurality of main channels may be provided, and one or a plurality of metering channels may be connected to each main channel.

  In the reaction container of the present invention, the main channel can be sealed, but an example in which the main channel can be sealed by opening and closing both ends of the main channel can be given. Here, “the both ends of the main channel can be opened and closed” means that other space is connected to the end of the main channel, and the end of the other space opposite to the main channel can be opened and closed This includes cases where For example, in the above embodiment, the flow path 13a, the liquid drain space 29, the drain space air vent flow path 23, and the flow path 23a correspond to the other spaces.

  In the reaction container of the present invention, the reaction chamber air vent channel can be hermetically sealed, but the reaction chamber air vent channel can be opened and closed at the end opposite to the reaction chamber. An example in which the extraction channel can be sealed can be given. Here, "the end of the reaction chamber air vent channel opposite to the reaction chamber is openable" means that the other end of the reaction chamber air vent channel opposite to the reaction chamber This includes the case where a space is connected and the end of the other space opposite to the reaction chamber air vent channel can be opened and closed. For example, in the above embodiment, the air drain space 31, the drain space air vent channel 25, and the channel 25a correspond to the other space.

  In such an embodiment, the liquid is introduced into the main channel and the metering channel, and then the liquid in the main channel is purged, and the liquid remaining in the metering channel is injected into the reaction chamber, and then the main stream. Both ends of the channel and the end of the reaction chamber air vent channel opposite to the reaction chamber are closed, and the main channel and the reaction chamber air vent channel are sealed.

  The present invention can be used for measurement of various chemical reactions and biochemical reactions.

Claims (18)

A reaction plate with multiple reaction chambers for reacting the sample;
An elastic plate placed on a reaction plate so as to close an opening of the reaction chamber, and the elastic plate is configured to supply a solution containing a sample to the reaction chamber on a surface facing the reaction plate. The flow path includes a main flow path that is common to all reaction chambers and individual flow paths that branch from the main flow path and connect to each reaction chamber. An elastic plate composed of an injection channel provided between the channel and the metering channel and the reaction chamber;
A holding plate disposed on the elastic plate to fix the elastic plate to the reaction plate;
A fixing mechanism provided between the reaction plate, the elastic body plate, and the holding plate, and fixing the elastic body plate to the reaction plate by the holding plate while maintaining liquid tightness;
Equipped with a,
The elastic plate further includes a reaction chamber air vent channel connected to the reaction chamber,
A reaction vessel in which the main channel, the individual channel, and the reaction chamber air vent channel are closed as a whole . The fixing mechanism is
A plurality of retaining protrusions provided on the reaction plate side surface of the holding plate;
A through hole that is opened in the elastic plate and allows the protrusion to pass through;
It consists of a hole that is opened in the reaction plate and into which the protrusion is inserted,
2. The protrusion and the hole are positioned and dimensioned so that the holding plate is positioned with respect to the reaction plate, and the elastic plate is fixed with respect to the reaction plate by the holding plate in a liquid-tight manner. The reaction container according to 1. The fixing mechanism is
On the surface of the holding plate on the reaction plate side, provided at least in the outer peripheral region outside the region where each flow path is formed, and having a claw portion protruding in one direction on the side of the tip portion, A plurality of fixing protrusions made of the same material as the holding plate;
A through hole that is provided at a position corresponding to the fixing protrusion on the elastic plate and allows the fixing protrusion to pass through;
The reaction plate is provided at a position corresponding to the fixing protrusion, and includes a hole into which the fixing protrusion is inserted to engage with the claw portion,
The reaction container according to claim 1, wherein the fixing protrusion and the hole are dimensioned so that the elastic plate is fixed to the reaction plate in a liquid-tight manner by a holding plate. The fixing protrusion is provided only in the outer peripheral region,
On the reaction plate side surface of the holding plate, a region inside the outer peripheral region is provided with a plurality of sealing projections having the same material as the holding plate without a claw at the tip.
The elastic plate is provided with a through-hole for passing the sealing projection at a position corresponding to the sealing projection,
The reaction container according to claim 3, wherein the reaction plate is provided with a hole having an inner diameter equal to the outer diameter of the sealing projection at a position corresponding to the sealing projection.   The elastic plate is formed with a concave or convex portion for positioning on the surface facing the holding plate, and the holding plate is located at a position corresponding to the concave or convex portion on the surface facing the elastic plate. The reaction container according to any one of claims 1 to 4, wherein a positioning convex portion or concave portion fitted into the concave portion or convex portion is formed.   The injection channel is narrower than the metering channel, and does not pass liquid at the liquid introduction pressure when the liquid is introduced into the main channel and the metering channel and at the purge pressure when the liquid in the main channel is purged. The reaction container according to any one of claims 1 to 5, wherein the reaction container is set to a size having a flow path resistance that allows liquid to pass through in a pressurized state. The reaction vessel according to any one of claims 1 to 6, wherein a contact angle of the injection channel with respect to water droplets is 90 degrees or more, and an area of a boundary between the injection channel and the metering channel is 1 to 10000000 µm ² . The main channel is formed with a narrowed portion downstream of the branching portion to each individual channel, and the channel resistance of the narrowed channel is less than the channel resistance of the metering channel. The reaction container according to any one of claims 1 to 7 , which is set so as to be large and smaller than a flow path resistance of the injection flow path. The reaction plate may inject a sample from the outside via the sample introduction port provided so as to be sealed according to any one of the main channel sample introduction portion connected claim 1, further comprising a to 8 Reaction vessel. Wherein the sample inlet can be penetrated by the sharp dispensing instrument tip, and when withdrawing the dispensing device after penetrating according to the through hole to claim 9, which is sealed by an elastic member which can be closed by an elastic Reaction vessel. The reaction container according to claim 9 or 10 , wherein the sample introduction part forms a container, and a sample pretreatment liquid or a reagent is sealed in the container in advance. The reaction plate further includes a reagent container that contains a reagent used for the reaction of the sample solution and is sealed with a film, or has a cap that can be opened and closed so that the reagent can be injected. Item 12. The reaction container according to any one of Items 1 to 11 . The reaction container according to any one of claims 1 to 12 , wherein the reaction plate also includes a gene amplification container for performing a gene amplification reaction. The reaction container according to any one of claims 1 to 13 , further comprising a syringe including a cylinder and a plunger disposed in the cylinder. The reaction container according to any one of claims 1 to 14 , further comprising a switching valve for switching and connecting the syringe to the main channel and another channel. The reaction vessel according to claim 15 , wherein the switching valve is a rotary valve. The reaction chamber according to any one of claims 1 to 16 , wherein the reaction chamber is made of a light-transmitting material so that optical measurement can be performed from the bottom thereof. The reaction container according to any one of claims 1 to 17 , wherein the reaction chamber includes a probe that reacts with a gene to be detected.

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