JP4828399B2 - Inspection kit, manufacturing method of inspection kit, and inspection method - Google Patents

Description

  The present invention relates to a chemical substance inspection technique, and more particularly, to an inspection kit, a manufacturing method of the inspection kit, and an inspection method.

In a living body, a plurality of biomolecules react in a complex manner to form a reaction system. Therefore, in order to elucidate the functions of individual biomolecules, it is important to search for other biomolecules that react specifically. For example, to elucidate the function of a receptor, a ligand that binds to the receptor is searched. In exploratory research, a plurality of beads each modified with a receptor are dammed up by a filter provided in a channel of a substrate, and a candidate substance for a ligand is caused to flow through the channel (see, for example, Patent Document 1). In addition, immunoassays that utilize high selectivity and high affinity of antigen-antibody reactions are widely used as analytical methods for trace substances such as proteins and hormones in biological samples, and are used in fields such as medical diagnosis and biochemical research. It is an indispensable analytical method. In an immunoassay, a plurality of beads each modified with an antibody against an antigen are dammed up by a filter provided in the channel of the substrate, and the antigen is allowed to flow through the channel (see, for example, Patent Document 2). The smaller the particle size of each of the plurality of beads, the larger the sum of the surface areas of the plurality of beads, so that it becomes easier to detect the reaction between the receptor and the ligand or the antigen and the antibody. However, each of the plurality of beads cannot be dammed up by the filter unless the particle diameter is made larger than the water guide portion of the filter. Since the filter is formed by molding glass or resin, there is a limit to reducing the water conveyance portion. Therefore, there is a limit in reducing the particle size of each of the plurality of beads that are blocked by the filter.
Japanese Unexamined Patent Publication No. 2006-201091 JP 2001-4628

  An object of the present invention is to provide a test kit, a test kit manufacturing method, and a test method that can test a chemical reaction using minute beads.

In order to achieve the above object, the first feature of the present invention is that: And (c) a plurality of damming beads that are blocked by the filter in the flow path, and (d) a size that can pass through each of the water conduits, and is blocked by a plurality of damming beads in the flow path. A plurality of test beads modified with a capture substance that captures the detected test substance, and from the upstream to the downstream in the flow direction of the test substance, a plurality of test beads, a plurality of damming beads, and a filter The gist is that the test kits are arranged in this order . In the inspection kit according to the first feature of the present invention, a plurality of inspection beads each smaller than the water conveyance portion of the filter that has a processing limit to be reduced are blocked by the flow path. The smaller each of the plurality of test beads blocked in the flow path, the greater the total surface area of each of the plurality of test beads. Therefore, when chemical substances react with each other on the surface of a plurality of test beads, the reaction that occurs per unit volume of the flow path increases, and the detection of the reaction becomes easy.

The second feature of the present invention includes (a) a step of flowing a plurality of damming beads in a flow path provided with a filter, and (b) a step of damaging a plurality of damming beads with a filter; C) a step of flowing a plurality of test beads each having a size capable of passing through the water conduit of the filter into the flow path; and (d) a plurality of test beads being dammed with a plurality of damming beads. A step of damming; and (e) a step of modifying each of the plurality of test beads with a capture substance that captures the test substance, wherein the plurality of test beads are directed from upstream to downstream in the flow direction of the test substance. The gist of the present invention is a method of manufacturing a test kit in which a plurality of damming beads and a filter are arranged in this order .

The third feature of the present invention is (a) a step of modifying each of a plurality of test beads with a capture substance, and (b) a step of flowing a plurality of damming beads in a flow path provided with a filter; (C) damming a plurality of damming beads with a filter; (d) flowing a plurality of test beads having a size capable of passing through the water-conducting part of the filter through the flow path; ) A step of damaging a plurality of test beads with a plurality of damming beads blocked; (f) a step of labeling a test substance captured by the capture substance with a labeling reagent; Flowing a test substance, capturing the test substance with a capture substance that modifies each of the plurality of test beads, and (h) detecting a labeling reagent that labels the test substance captured with the capture substance , Examination The gist of the present invention is an inspection method in which a plurality of inspection beads, a plurality of blocking beads, and a filter are arranged in this order from upstream to downstream in the material flow direction .

The fourth feature of the present invention is (a) a step of modifying each of a plurality of test beads with a capture substance, and (b) a step of flowing a plurality of damming beads in a flow path provided with a filter; (C) damming a plurality of damming beads with a filter; (d) flowing a plurality of test beads having a size capable of passing through the water-conducting part of the filter through the flow path; ) Steps of damaging multiple test beads with dammed multiple damming beads, and (f) Flowing the test substance captured by the capture substance into the flow path and modifying each of the multiple test beads (G) a step of labeling an anchor substance that captures a test substance with a labeling reagent, and (h) a flow of the anchor substance labeled in the flow channel, and the test substance with the labeled anchor substance And (b) detecting a labeling reagent for labeling the anchor substance that has captured the test substance, from the upstream to the downstream in the flow direction of the test substance, and a plurality of test beads, The gist of the present invention is that it is an inspection method in which the damming beads and the filter are arranged in this order .

  ADVANTAGE OF THE INVENTION According to this invention, the test kit which can test | inspect a chemical reaction using a micro bead, the manufacturing method of a test kit, and a test method can be provided.

  Next, embodiments of the present invention will be described with reference to the drawings. In the following description of the drawings, the same or similar parts are denoted by the same or similar reference numerals. The embodiments shown below exemplify apparatuses and methods for embodying the technical idea of the present invention, and the technical idea of the present invention specifies the arrangement of components and the like as follows. Not what you want. The technical idea of the present invention can be variously modified within the scope of the claims.

The inspection kit according to the embodiment of the present invention includes a transparent substrate 10 provided with the flow path 11 shown in FIG. 1 and a filter 131 disposed in the flow path 11 of the substrate 10. For example, the channel 11 is a groove provided in the substrate 10 as shown in FIG. 2 which is a cross-sectional view seen from the AA direction. At one end of the flow path 11 of the substrate 10 shown in FIG. 1, an injection well 21 into which a solution to be flowed through the flow path 11 is injected is provided. Further, at the other end of the flow path 11 of the substrate 10, a pouring well 22 for storing the solution flowing through the flow path 11 is provided. A cover plate 60 is disposed on the substrate 10 as shown in FIG. As shown in FIG. 4 and FIG. 5 which is a cross-sectional view seen from the AA direction, the filter 131 is provided with a plurality of water guide portions 31a, 31b, 31c, 31d, 31e, 31f, 31g, and 31h that allow the solution to pass therethrough. ing. Each of the cross-sectional shape of the plurality of the water conduit 31a~31h is rectangular and the shorter side length d ₁ is, for example, 18 [mu] m. Transparent materials such as silicon oxide (SiO ₂ ) and acrylic resin can be used as materials for the substrate 10, the filter 131, and the cover plate 60, respectively. The substrate 10 and the filter 131 may be integrated. The substrate 10 and the filter 131 provided with the flow path 11 can be manufactured by an etching method, an injection molding method, or the like.

The inspection kit according to the embodiment has a size that allows each of the plurality of damming beads 41a, 41b, 41c,... Dammed by the filter 131 in the flow path 11 and the plurality of water guiding portions 31a to 31h to pass therethrough. And a plurality of test beads 51a, 51b, 51c,... Dammed by the plurality of damming beads 41a to 41c in the flow path 11. The diameter of each of the plurality of dam bead for preventing 41a~41c is larger than the length d ₁ of each of the short sides of the plurality of water guiding portions 31 a to 31 h, such as 30 [mu] m. The diameter of each of the plurality of test beads 51a~51c is smaller than the length d ₁ of each of the short sides of the plurality of water guiding portions 31 a to 31 h, such as 10 [mu] m. As materials of the plurality of damming beads 41a to 41c and the plurality of inspection beads 51a to 51c, silicon oxide (SiO ₂ ), latex, or the like can be used.

The plurality of test beads 51a~51c respective surface, for example, aldehyde (-CHO) group, a carboxyl (-COOH) group, and amino (-NH ₂₎ shown in FIG. 6 functional group 53, such as a group is introduced Yes. The functional group 53 includes, for example, a crosslinking agent 54 such as bissulfosuccinimidyl suberate (BS ³ ) that reacts with an amino (—NH ₂ ) group at both ends shown in FIG. Are combined as shown in Furthermore, for example, a capture substance 55 shown in FIG. 9 that is a protein such as a receptor, a ligand, an antagonist, an antibody, or an antigen binds to the cross-linking agent 54 via an amino (—NH ₂ ) group of lysine (Lys). is doing.

In addition, as cross-linking agent 54, disuccinimidyl suberate (DSS), dimethyl suberimidate (HCl: DMS), disuccinimid, which reacts with amino (-NH ₂ ) groups at both ends. Disuccinimidyl glutarate (DSG), Loman's Reagent, 3, 3'-dithiobissulfosuccinimidyl propionate (3,3'-Dithiobis [sulfo succinimidyl propionate]: DTSSP), and ethylene Glycol bis succinimidyl succinate (EGS) etc. can be used.

When the capture material 55 contains aspartic acid (Asp) and glutamic acid (Glu) having a carboxyl (-COOH) group, it reacts with an amino (-NH ₂ ) group and a carboxyl (-COOH) group as a crosslinking agent 1 -Ethyl-3- [3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) or the like can be used.

When the capture substance 55 includes cysteine (Cys) having a thiol (-SH) group, m-maleimidobenzyl-N that reacts with an amino (-NH ₂ ) group and a thiol (-SH) group as a crosslinking agent 54. -Hydroxysuccinimide ester (m-Maleimidobenzyl-N -hydroxy succinimide ester: MBS), Succinimidyl 4-[N-maleimidomethyl]-Cyclohexane-1-Carboxylate (Succinimidyl 4-[N-maleimidomethyl]-Cyclohexane-1-carboxylate: SMCC), succinimidyl 4-[p-maleimidophenyl]-butyrate (Succinimidyl 4-[p-maleimidophenyl]-buthrate: SMPB), N-succinimidyl 3- (2-pyridyldithio) propionate (N-Succinimidyl 3-[2- pyridyldithio] propionate: SPDP), N- (γ-Maleimidobutyryloxy) sulfosuccinimide ester (Sulfo-GMBS), sulfosuccinimidyl 6-[3 '(2 -Pyridyldithio) -propionamide] hexanoate (Sulfo succinimidyl 6- [3 '(2-pyridyldithio) -propionamide) hexanoate: Sulfo-LC-SPDP), m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester (m-Maleimidebenzoyl) -N-hydoroxysulfo-succinimide ester: Sulfo-MBS), Sulfosuccinimidyl-4- (N-maleimidomethyl) cyclohexane-1-carboxylate (Sulfo succinimidyl 4 [N-maleimidomethyl]-cyclohexane-1-carboxylate: Sulfo -SMCC), sulfosuccinimidyl-4- (p-maleimidophenyl) butyrate (Sulfo succinimidy 4- [p-maleimidophenyl] -butyrate: Sulfo-SMPB), etc. can be used.

  Next, the inspection method according to the embodiment will be described.

(a) First, as shown in FIG. 6, 100 μl of a test bead-containing solution including a plurality of test beads 51a to 51c each having a functional group 53 such as an amino (—NH ₂ ) group introduced therein is prepared. Next, 500 μl of pH 8.0 phosphate buffer containing bissulfosuccinimidyl suberate as a crosslinker 54 shown in FIG. 7 at a concentration of 5 mmol / l is added to the test bead-containing solution and mixed at room temperature for 10 minutes. As shown in FIG. 8, N-hydroxy sulfosuccinimide is introduced into the surface of each of the plurality of test beads 51a to 51c.

  (b) 400 μl of pH 8.0 phosphate buffer containing anti-lysozyme antibody as capture substance 55 at a concentration of 4.15 mg / ml was added to the test bead-containing solution, mixed at 4 ° C. for 2 hours, and as shown in FIG. The capture substance 55 is bound to each of the plurality of test beads 51a to 51c. Next, bovine serum albumin (BSA) is added to the test bead-containing solution to block the unreacted functional group 53 and the crosslinking agent 54.

  (c) A substrate 10 provided with a filter 131 in the flow path 11 shown in FIG. 10 is prepared. Next, a blocking bead-containing liquid including a plurality of blocking beads 41a to 41c is caused to flow through the flow path 11, and the plurality of blocking beads 41a to 41c are blocked by the filter 131 as shown in FIG. Thereafter, the test bead-containing liquid is caused to flow through the flow path 11, and the plurality of test beads 51a to 51c, to which the capture substances 55 are respectively bonded, are blocked by the plurality of blocking beads 41a to 41c as shown in FIG. FIG. 12 shows the filter 131, the plurality of damming beads 41a to 41c, and the plurality of inspection beads 51a to 51c in the flow path 11 observed in the bright field.

  (d) Dissolve 1 mg of lysozyme as a test substance to be captured by the anti-lysozyme antibody that is the capture substance 55 in 500 μl of pH 8.0 phosphate buffer to prepare an antigen solution. As a labeling reagent, 0.1 mg of N-hydroxysuccinimide-fluorescein isothiocyanate (NHS-FITC) is dissolved in 500 μl of pH 8.0 phosphate buffer to prepare a labeling solution. Next, the antigen solution and the labeling solution are mixed for 1 hour to prepare a test solution containing the test substance labeled with the labeling reagent. Thereafter, the unreacted labeling reagent is removed from the test solution using a gel filtration column or the like.

  (e) 100 μl of a test solution containing a test substance labeled with a labeling reagent at a concentration of 1 μg / ml is passed through the flow channel 11 shown in FIG. 4 and the capture substance 55 bound to each of the plurality of test beads 51a to 51c. Then, the test substance labeled with the labeling reagent is captured. Next, 200 μl of a phosphate buffer containing a surfactant (Tween 20) at a volume concentration of 0.05% is flowed into the channel 11 to wash away the test substance that has not been captured. Thereafter, excitation light with a wavelength of 495 nm is irradiated, and the fluorescence of the labeling reagent is observed as shown in FIG. Thereafter, the concentration of the test substance is calculated based on the intensity of the fluorescence, and the inspection method according to the embodiment is completed.

  In the conventional method in which the beads modified with the antibody are directly dammed to the filter in the flow path, the beads have to be of a size that cannot pass through the water guide portion of the filter. However, there is a limit to reducing the water conveyance part of the filter formed by the etching method and the injection molding method. On the other hand, according to the inspection method according to the embodiment, a plurality of inspection beads 51a to 51c smaller than each of the plurality of water guiding portions 31a to 31h of the filter 131 shown in FIG. Is possible. Therefore, it is possible to increase the total surface area of the plurality of test beads 51a to 51c blocked per unit volume of the flow path without being limited by the processing limit of the plurality of water guide portions 31a to 31h. Therefore, since the binding reaction between the capture substance and the test substance generated per unit volume increases, the binding reaction can be easily detected.

(Modification)
For example, the test substance may flow through the channel 11 without being labeled with a labeling reagent. In this case, an anchor substance that is a substance that captures the test substance is prepared, and the anchor substance is labeled with a labeling reagent. Next, the labeled anchor substance is caused to flow through the flow path 11, and the test substance captured on each of the plurality of test beads 51a to 51c is captured. Thereafter, a labeling reagent for labeling the anchor substance may be detected. As the anchor substance, an antibody, a receptor, an aptamer, or the like can be used. Further, the anchor substance does not necessarily have to directly capture the test substance. For example, the test substance may be captured with an antibody that is modified with biotin and binds to the test substance, and streptavidin modified with a labeling reagent is allowed to flow as an anchor substance in the flow path to bind to biotin.

(Other embodiments)
As mentioned above, although this invention was described by embodiment, it should not be understood that the description and drawing which form a part of this indication limit this invention. For example, in the embodiments, it has been described that the capture substance is a protein. However, the capture substance is not limited to nucleic acids such as deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and peptide nucleic acid (PNA), or biomolecules. It may be a substance. Cy3 or the like can also be used as a labeling reagent. Further, in FIG. 5, it has been described that the cross-sectional shape of each of the plurality of water guide portions 31a to 31h is a rectangle, but it is needless to say that the shape is not limited thereto. As indicated above, various alternative embodiments, examples and operational techniques will be apparent to those skilled in the art from this disclosure. Therefore, the technical scope of the present invention is defined only by the invention specifying matters according to the scope of claims reasonable from the above description.

It is a top view of a test kit according to an embodiment of the present invention. It is the 1st sectional view of the inspection kit concerning an embodiment of the invention. It is a 2nd sectional view of the inspection kit concerning an embodiment of the invention. It is an enlarged top view of the inspection kit according to the embodiment of the present invention. It is an expanded sectional view of the inspection kit concerning an embodiment of the invention. It is a schematic diagram of the test bead according to the embodiment of the present invention. It is a chemical formula of the crosslinking agent which concerns on embodiment of this invention. It is a schematic diagram of the bead for a test | inspection couple | bonded with the crosslinking agent which concerns on embodiment of this invention. It is a schematic diagram of the bead for a test | inspection couple | bonded with the capture substance which concerns on embodiment of this invention. It is the 1st top view showing the manufacturing process of the inspection kit concerning the embodiment of the invention. It is a 2nd top view which shows the manufacturing process of the test | inspection kit which concerns on embodiment of this invention. It is the 1st top view showing the process of the inspection method concerning an embodiment of the invention. It is a 2nd top view which shows the process of the inspection method which concerns on embodiment of this invention.

Explanation of symbols

10 ... Board
11 ... Flow path
21 ... Infusion well
22 ... Pouring well
31a-31h ... Water conveyance part
41a-41c ... damming beads
51a-51c ... Beads for inspection
53… functional group
54 ... Crosslinking agent
55… Captured substance
60 ... Lid
131 ... Filter

Claims (25)

A substrate provided with a flow path;
A filter disposed in the flow path of the substrate, and provided with a water guiding portion for allowing the solution to pass through;
A plurality of damming beads dammed to the filter in the flow path;
A plurality of test beads each having a size capable of passing through the water conduit and modified with a capture substance that captures the test substance blocked by the plurality of damming beads in the flow path. ,
A test kit, wherein the plurality of test beads, the plurality of blocking beads, and the filter are arranged in this order from upstream to downstream in the flow direction of the test substance.   The test kit according to claim 1, wherein the capture substance is an antibody.   The test kit according to claim 1, wherein the capture substance is a receptor.   The test kit according to claim 1, wherein the capture substance is an aptamer.   Each of the plurality of damming beads has a diameter larger than the minimum inner diameter of the water guide section when compared with the minimum inner diameter of the water guide section in a cross section obtained by orthogonally passing in the longitudinal direction of the flow path of the water guide section. The test kit according to claim 1, wherein each of the plurality of test beads has a diameter smaller than a minimum inner diameter of the water guide portion. Flowing a plurality of damming beads through a flow path provided with a filter;
Damming the plurality of damming beads with the filter;
Flowing a plurality of test beads having a size capable of passing through the water guide portions of the filter in the flow path;
Damaging the plurality of test beads with the dammed plurality of damming beads;
Modifying each of the plurality of test beads with a capture substance that captures the test substance,
A method for producing a test kit, wherein the plurality of test beads, the plurality of blocking beads, and the filter are arranged in this order from upstream to downstream in the flow direction of the test substance.   The method for producing a test kit according to claim 6, wherein the capture substance is an antibody.   The method for producing a test kit according to claim 6, wherein the capture substance is a receptor.   The method for producing a test kit according to claim 6, wherein the capture substance is an aptamer.   Each of the plurality of damming beads has a diameter larger than the minimum inner diameter of the water guide section when compared with the minimum inner diameter of the water guide section in a cross section obtained by orthogonally passing in the longitudinal direction of the flow path of the water guide section. The method for manufacturing an inspection kit according to any one of claims 6 to 9, wherein each of the plurality of inspection beads has a diameter smaller than a minimum inner diameter of the water guide portion. Modifying each of the plurality of test beads with a capture substance;
Flowing a plurality of damming beads through a flow path provided with a filter;
Damming the plurality of damming beads with the filter;
Flowing the plurality of test beads having a size capable of passing through the water guide portions of the filter in the flow path;
Damaging the plurality of test beads with the dammed plurality of damming beads;
Labeling a test substance captured by the capture substance with a labeling reagent;
Flowing the test substance through the flow channel, and capturing the test substance with the capture substance that modifies each of the plurality of test beads;
Detecting the labeling reagent that labels the test substance captured by the capture substance,
An inspection method, wherein the plurality of inspection beads, the plurality of damming beads, and the filter are arranged in this order from upstream to downstream in the flow direction of the test substance.   The inspection method according to claim 11, wherein the capture substance is an antibody.   The inspection method according to claim 11, wherein the capture substance is a receptor.   The inspection method according to claim 11, wherein the capture substance is an aptamer.   15. The inspection method according to claim 11, wherein the labeling reagent is a fluorescent reagent. The test method according to any one of claims 11 to 15 , further comprising a step of calculating a concentration of the test substance based on the detected labeling reagent. Modifying each of the plurality of test beads with a capture substance;
Flowing a plurality of damming beads through a flow path provided with a filter;
Damming the plurality of damming beads with the filter;
Flowing the plurality of test beads having a size capable of passing through the water guide portions of the filter in the flow path;
Damaging the plurality of test beads with the dammed plurality of damming beads;
Flowing a test substance to be captured by the capture substance into the flow path, and capturing with the capture substance to modify each of the plurality of test beads;
Labeling an anchor substance that captures the test substance with a labeling reagent;
Flowing the labeled anchor substance through the flow path, and capturing the test substance with the labeled anchor substance;
Detecting the labeling reagent for labeling the anchor substance that has captured the test substance,
An inspection method, wherein the plurality of inspection beads, the plurality of damming beads, and the filter are arranged in this order from upstream to downstream in the flow direction of the test substance.   The inspection method according to claim 17, wherein the capture substance is an antibody.   The inspection method according to claim 17, wherein the capture substance is a receptor.   The inspection method according to claim 17, wherein the capture substance is an aptamer.   21. The inspection method according to claim 17, wherein the anchor substance is an antibody.   The inspection method according to any one of claims 17 to 20, wherein the anchor substance is a receptor.   21. The inspection method according to claim 17, wherein the anchor substance is an aptamer.   24. The inspection method according to claim 17, wherein the labeling reagent is a fluorescent reagent.   The test method according to any one of claims 17 to 24, further comprising a step of calculating a concentration of the test substance based on the detected labeling reagent.