JP4815545B1 - Carbon dioxide gas retention agent and container-packed carbonated drink - Google Patents

Abstract

The present invention provides a carbon dioxide gas retention agent excellent in retention of carbon dioxide gas, a packaged carbonated drink containing the same, and a method for retaining carbon dioxide in a packaged carbonated drink.
A carbon dioxide gas retention agent comprising a non-polymer catechin as an active ingredient. Non-polymer catechins contain epigallocatechin gallate, and the content of epigallocatechin gallate in the non-polymer catechins is preferably 30% by mass or more. Furthermore, it contains stevia extract as an active ingredient, and the ratio [S] / [C of the content [S] (mass%) of stevia extract to the content [C] (mass%) of non-polymer catechins ] Is preferably 0.02 to 5.
[Selection figure] None

Description

  The present invention relates to a carbon dioxide gas retaining agent, a container-packed carbonated drink containing the same, and a method for retaining carbon dioxide in a container-packed carbonated drink.

  Carbonated beverages are soft drinks that can obtain a refreshing feeling not only by their taste but also by the stimulation of carbon dioxide gas when consumed, and have been widely used in the past. However, carbon dioxide gas has a property that it easily escapes from beverages, and a carbonated beverage from which carbon dioxide gas has escaped is unpreferably undesirable. In particular, small-sized PET bottle carbonated beverages that have become popular in recent years are often left uncooled for a long time after opening until they are drunk, so that carbon dioxide can escape during this period. Is a problem.

  In the actual situation as described above, there has been proposed a carbonated beverage containing a high-intensity sweetener, a specific amount of polymerized catechin and caffeine, respectively, and improving the bubble feeling of carbon dioxide (Patent Document 1).

JP 2010-142129 A

  However, in the carbonated beverage described in Patent Document 1, the retention of carbon dioxide gas after opening, particularly the retention of carbon dioxide gas when left untreated for a long time after opening, is insufficient. There has been a demand for a carbon dioxide retaining agent that can sufficiently retain carbon dioxide in a carbonated beverage even under conditions.

  Then, an object of this invention is to provide the carbon dioxide holding agent excellent in the holding | maintenance of a carbon dioxide gas, the container-packed carbonated drink which mix | blended this, and the carbon dioxide-gas holding method in a container-packed carbonated drink.

  In order to achieve the above object, first, the present invention provides a carbon dioxide gas retention agent characterized by containing a non-polymer catechin as an active ingredient (Invention 1).

  According to the said invention (invention 1), when it mix | blends with a container-packed carbonated drink, the carbon dioxide holding agent which can hold | maintain a carbon dioxide effectively can be obtained.

  In the above invention (Invention 1), the non-polymer catechins include epigallocatechin gallate, and the content of the epigallocatechin gallate in the non-polymer catechins is 30% by mass or more. Preferred (Invention 2).

  The carbon dioxide holding agent according to the above inventions (Inventions 1 and 2) may further contain polymerized catechin. In this case, the ratio [P] / [C] of the content [P] (mass%) of the polymerized catechin to the content [C] (mass%) of the non-polymer catechins is 0.25 or less. Is preferred (Invention 3).

  In the said invention (invention 1-3), stevia extract is further contained as an active ingredient, The content [S] of the said stevia extract with respect to content [C] (mass%) of the said non-polymer catechins ( (% By mass) [S] / [C] is preferably 0.02 to 5 (Invention 4).

  Secondly, the present invention provides a packaged carbonated beverage characterized by blending the carbon dioxide retaining agent of the above invention (Inventions 1 to 3) (Invention 5).

  According to the said invention (invention 5), the container-packed carbonated drink excellent in holding | maintenance of a carbon dioxide gas can be obtained. In the said invention (invention 5), it is preferable that the gas volume of a carbon dioxide gas is 1.8-2.85, or 3.15-4.0 (invention 6).

  Thirdly, the present invention provides a packaged carbonated beverage characterized by blending the carbon dioxide retaining agent of the above invention (Invention 4) (Invention 7).

  According to the said invention (invention 7), the container-packed carbonated drink excellent in holding | maintenance of a carbon dioxide gas can be obtained. In the said invention (invention 7), it is preferable that the gas volume of a carbon dioxide gas is 1.8-4.0 (invention 8).

  In the said invention (invention 5-8), the said carbon dioxide gas holding | maintenance agent is mix | blended so that content of the said non-polymer catechin in the said packaged carbonated drink may be 0.001-0.035 mass%. Is preferable (Invention 9).

  Third, the present invention is a method for holding carbon dioxide in a carbonated carbonated beverage, wherein the carbonated beverage containing non-polymer catechins as an active ingredient is contained in the carbonated carbonated beverage. A method is provided (Invention 10).

  In the above invention (Invention 10), the non-polymer catechins contain epigallocatechin gallate, and the content of the epigallocatechin gallate in the non-polymer catechins is 30% by mass or more. It is preferable to contain the epigallocatechin gallate (Invention 11).

  In the method for retaining carbon dioxide in a carbonated carbonated beverage according to the above invention (Inventions 10 and 11), the carbonated carbonated beverage may further contain a polymerized catechin. In this case, in the container-packed carbonated beverage, the non-polymer so that the ratio of the content (mass%) of the polymerized catechin to the content (mass%) of the non-polymer catechins is 0.25 or less. It is preferable to contain catechins and the polymerized catechin (Invention 12).

  The carbon dioxide retaining agent of the present invention can effectively retain carbon dioxide when blended in a container-packed carbonated beverage. Moreover, the container-packed carbonated drink of this invention is excellent in holding | maintenance of a carbon dioxide gas.

Hereinafter, embodiments of the present invention will be described.
The carbon dioxide gas retaining agent according to one embodiment of the present invention contains non-polymer catechins as active ingredients. Moreover, the container-packed carbonated drink which concerns on one Embodiment of this invention contains the said carbon dioxide-gas holding | maintenance agent.

  In the present specification, non-polymer catechins are (±) -catechin (C), (±) -gallocatechin (GC), and epithelium (−)-epicatechin (EC), (− ) -Epigallocatechin (EGC), and gallic acid esters (gallates) thereof (-)-catechin gallate (Cg), (-)-gallocatechin gallate (GCg), (-)-epicatechin gallate ( ECg) and 8 types of catechin compounds (-)-epigallocatechin gallate (EGCg).

  The carbon dioxide gas retaining agent according to the present embodiment contains non-polymer catechins as an active ingredient, so that when mixed in a container-packed carbonated drink, the container-packed carbonated drink effectively retains the carbon dioxide gas. Can do. Therefore, when the container-packed carbonated drink is drunk, the refreshing feeling by the carbon dioxide gas is sustained, and the carbonated drink is preferably preferred.

  In addition, in the non-polymer catechins contained in the carbon dioxide retention agent according to this embodiment, one or more catechin compounds selected from the group consisting of Cg, GCg, ECg and EGCg which are gallate bodies Is preferably contained in an amount of 40% by mass or more, more preferably 90% by mass or more in the whole non-polymer catechins. In particular, EGCg is preferably contained in an amount of 30% by mass or more, more preferably 60% by mass or more in the whole non-polymer catechins.

  In addition, content of the 8 types of catechin compound mentioned above in this specification is the value measured by the high performance liquid chromatography method, and a specific measuring method is shown in the Example mentioned later. Moreover, content of the non-polymer catechin in this specification is the value which totaled each content of 8 types of catechin compounds measured in this way.

  The non-polymer catechins in the present embodiment may be those obtained by extraction from natural products containing non-polymer catechins (for example, green tea leaves) by a known method, or commercially available products are used. May be. As a commercial product containing non-polymer catechins satisfying the above-mentioned preferable conditions, Teafuran 90S (manufactured by ITO EN) can be exemplified.

  The carbon dioxide holding agent according to this embodiment may further contain a polymerized polyphenol, but in order to effectively hold the carbon dioxide in the container-packed carbonated beverage, the content is preferably as small as possible. Here, the polymerization polyphenol is a general term for compounds having a structure in which two or more polyphenols containing two or more phenolic hydroxyl groups in the molecule are linked. Examples of the polymerized polyphenol obtained from plants include polymerized catechins. Polymerized catechins have a structure in which a plurality of the above-mentioned non-polymer catechins are linked by light, enzyme or the like, such as EGCg dimer, EGCg trimer, EGC dimer, EGC trimer, etc. Can be illustrated.

  In the carbon dioxide gas retaining agent according to the present embodiment, the ratio [P] / [C] of the content [P] (mass%) of the polymerized catechin to the content [C] (mass%) of the non-polymer catechins is It is preferably 0.25 or less, and more preferably 0.2 or less. When [P] / [C] is within this range, carbon dioxide can be effectively retained in the container-packed carbonated beverage containing the carbon dioxide retaining agent according to the present embodiment. Here, [P] / [C] being 0.25 or less includes the case where [P] / [C] is 0, that is, the case where no polymerized catechin is contained. In addition, content [P] of the polymerization catechin in this specification can be measured by a well-known high performance liquid chromatograph method.

  In the carbon dioxide gas retaining agent according to the present embodiment, the ratio [P] / [E] of the content [P] (mass%) of the polymerized catechin to the content [E] of the EGCg is 0.34 or less. It is preferable that it is 0.28 or less. By having [P] / [E] within this range, carbon dioxide can be effectively retained in the container-packed carbonated beverage containing the carbon dioxide retaining agent according to the present embodiment. Here, [P] / [E] being 0.34 or less includes a case where [P] / [E] is 0, that is, a case where no polymerized catechin is contained.

  The carbon dioxide gas retaining agent according to the present embodiment preferably further contains a stevia extract as an active ingredient. By containing a stevia extract as an active ingredient, carbon dioxide can be effectively retained in a container-packed carbonated beverage containing the carbon dioxide retaining agent according to the present embodiment.

  Stevia (scientific name: Stevia rebaudiana) is a perennial plant from Asteraceae native to South America, and its extract is a glycoside with steviol as a skeleton (steviol glycoside) as a sweet component, stevioside, rebaudioside A, rebaudioside C, zulcoside A and the like are contained. The “stevia extract” in the present specification includes an extract obtained by using stevia as an extraction raw material, or a crudely purified product or a purified product thereof. Furthermore, in addition to the purified products of steviol glycosides such as stevioside, rebaudioside A, rebaudioside C, and zulcoside A contained in stevia extract, the crude product or purified product includes the above extract or Enzyme-treated stevia obtained by enzyme treatment of purified products and the like is also included. In addition, what was extracted by the method mentioned above may be used for the stevia extract in this embodiment, and a commercially available thing may be used for it.

  The stevia extract used in the carbon dioxide gas retaining agent of the present embodiment preferably contains rebaudioside A and / or stevioside obtained from the stevia extract by purification or enzymatic treatment. It is particularly preferable that the side A contains 95% by mass or more. When the content of rebaudioside A in the stevia extract is 95% by mass or more, the retention effect of carbon dioxide gas becomes more remarkable. A rebaudio J-100 (manufactured by Morita Chemical Co., Ltd.) can be exemplified as a commercially available stevia extract satisfying such preferable conditions.

  In the carbon dioxide gas retaining agent according to the present embodiment, the ratio [S] / [C of the content [S] (mass%) of the stevia extract to the content [C] (mass%) of the non-polymer catechins. ] Is preferably 0.1-5, more preferably 0.3-4, and particularly preferably 0.3-2.2. By containing the non-polymer catechins and stevia extract in the carbon dioxide gas retention agent so as to satisfy this range, the carbon dioxide gas is effectively retained in the packaged carbonated beverage containing the carbon dioxide gas retention agent according to this embodiment. Can be made. In particular, when the gas volume to be described later in the container-packed carbonated beverage is more than 2.85 and less than 3.15, if the carbon dioxide retaining agent according to this embodiment does not contain a stevia extract, the carbonation from the container-packed carbonated beverage It promotes gas escape. However, by adding a stevia extract to the carbon dioxide gas retaining agent so that [S] / [C] is in the above range, even if the gas volume of the packaged carbonated beverage is within the above range, the carbon dioxide gas is reduced. It can be effectively held.

  In addition, because [S] / [C] is in the above range, in the packaged carbonated beverage containing the carbon dioxide gas retaining agent according to the present embodiment, the astringency derived from non-polymer catechins is not felt, In addition, the sweetness derived from the stevia extract has a good finish and becomes a highly preferable container-packed carbonated beverage. In addition, content [S] of the stevia extract in this specification can be measured by a well-known high performance liquid chromatograph method.

  In the carbon dioxide gas retaining agent according to the present embodiment, the ratio [S] / [E] of the stevia extract content [S] to the EGCg content [E] is preferably 0.1-7. 0.3 to 5.4 is more preferable, and 0.3 to 3 is particularly preferable. When [S] / [E] is within this range, carbon dioxide gas can be effectively retained in the container-packed carbonated beverage containing the carbon dioxide gas retaining agent according to the present embodiment.

  In the carbon dioxide gas retaining agent according to the present embodiment, the ratio [R] / [of the content [R] (mass%) of rebaudioside A to the content [C] (mass%) of non-polymer catechins. C] is preferably 0.1 to 5, more preferably 0.2 to 3.8, and particularly preferably 0.2 to 2.1. When [R] / [C] is within this range, carbon dioxide gas can be effectively retained in the container-packed carbonated beverage containing the carbon dioxide gas retaining agent according to the present embodiment. In addition, the content [R] of rebaudioside A in the present specification can be measured by a known high performance liquid chromatography method.

  In the carbon dioxide gas retaining agent according to the present embodiment, the ratio [R] / [E] of the content [R] (mass%) of the rebaudioside A to the content [E] (mass%) of the EGCg. Is preferably 0.096 to 6.8, more preferably 0.28 to 5.2, and particularly preferably 0.28 to 2.9. When [R] / [E] is within this range, carbon dioxide gas can be effectively retained in the container-packed carbonated beverage containing the carbon dioxide gas retaining agent according to the present embodiment. In addition, the content [R] of rebaudioside A in the present specification can be measured by a known high performance liquid chromatography method.

  In the container-packed carbonated beverage according to the present embodiment, the carbon dioxide gas holding agent described above may be blended so that the content (% by mass) of the non-polymer catechins is 0.001 to 0.035% by mass. It is more preferable to mix | blend so that it may become 0.002-0.03 mass%, and it is especially preferable to mix | blend so that it may become 0.002-0.02 mass%. In the container-packed carbonated beverage according to this embodiment, the carbon dioxide gas is effectively retained by blending the carbon dioxide gas holding agent so that the content of the non-polymer catechins is in the above-described range, and the non-polymer The astringency derived from catechins is not felt.

  In the container-packed carbonated beverage according to this embodiment, the carbon dioxide gas holding agent described above is blended so that the content (mass%) of epigallocatechin gallate (EGCg) is 0.0007 to 0.026 mass%. It is more preferable that it is blended so as to be 0.0014 to 0.023 mass%, and it is particularly preferable that it is blended so as to be 0.0014 to 0.015 mass%. By compounding the carbon dioxide gas holding agent so that the EGCg content is in the above-described range, the carbon dioxide gas is effectively held and the flavor is excellent in the container-packed carbonated beverage according to this embodiment. .

  In addition, when the above-mentioned carbon dioxide gas retaining agent further contains a stevia extract, the container-packed carbonated beverage according to the present embodiment contains the stevia extract in a content of 0.003 to 0.05 mass%. Is preferable, 0.005 to 0.05% by mass is further preferable, 0.005 to 0.04% by mass is particularly preferable, and 0.005 to 0.022% by mass is extremely preferable. . By blending the carbon dioxide gas holding agent so that the content of stevia extract is in the above-described range, the carbon dioxide gas is effectively retained in the container-packed carbonated beverage according to this embodiment, and is derived from the stevia extract. The sweetness to be cut becomes good.

  Moreover, when the carbon dioxide holding agent mentioned above further contains rebaudioside A, in the container-packed carbonated beverage according to the present embodiment, the rebaudioside A is contained in an amount of 0.0028 to 0.048% by mass. The content is preferably 0.0048 to 0.048% by mass, more preferably 0.0048 to 0.039% by mass, and 0.0048 to 0.022% by mass. It is very preferable. In the container-packed carbonated beverage according to the present embodiment, the carbon dioxide gas is effectively retained by adding the carbon dioxide gas retaining agent so that the content of the rebaudioside A is in the range described above, and the rebaudio The sweetness derived from Side A is good after cutting.

  In the container-packed carbonated beverage according to the present embodiment, when the above-mentioned carbon dioxide gas retaining agent does not contain a stevia extract, the gas volume of the carbon dioxide gas is 1.8 to 2.85 or 3.15 to 4 0.0 is preferred. When the gas volume of the carbon dioxide gas is within this range, the carbon dioxide gas is effectively retained in the container-packed carbonated beverage containing the carbon dioxide retaining agent that does not contain stevia extract. Moreover, since the gas volume of the carbon dioxide gas is in the above-described range, it is possible to obtain a refreshing feeling of the carbon dioxide gas and thereby a good feeling over the throat. On the other hand, when the gas volume of the carbon dioxide gas is more than 2.85 and less than 3.15, the outgassing of the carbon dioxide gas from the packaged carbonated beverage is promoted by containing the non-polymer catechins. In addition, the gas volume of the carbon dioxide gas in this specification means what divided | segmented the volume of the carbon dioxide gas melt | dissolved in the carbonated beverage in 20 degreeC by the volume of the carbonated beverage, and a specific measuring method is mentioned later. It is shown in the examples.

  In the container-packed carbonated beverage according to the present embodiment, when the carbon dioxide retaining agent described above contains a stevia extract, the gas volume of the carbon dioxide gas is preferably 1.8 to 4.0. When the above-mentioned carbon dioxide gas holding agent contains a stevia extract, not only when the gas volume of the carbon dioxide gas is 1.8 to 2.85 or 3.15 to 4.0, but more than 2.85 and 3.15. Even if it is less than this, carbon dioxide can be effectively held in the packaged carbonated beverage. Further, since the carbon dioxide gas volume is in this range, it is possible to obtain a refreshing feeling of the carbon dioxide gas and a good feeling over the throat. In addition, it is more preferable that the gas volume of the carbon dioxide gas is 1.8 to 2.85 or 3.15 to 4.0 because the carbonated gas can be more effectively retained in the packaged carbonated beverage.

  In addition, the container-packed carbonated drink according to the present embodiment is water or a material (component) contained in a known drink, in addition to the above-mentioned carbon dioxide retaining agent, for example, sweeteners other than vitamins and stevia extract, You may mix | blend a sour agent, a fragrance | flavor, a mineral component, a pigment component, a functional component, etc. in the range which does not impair the effect by this embodiment.

  The water may be water that is suitable for drinking, and examples thereof include pure water, hard water, soft water, ion exchange water, and deaerated water obtained by deaeration of these waters.

  Examples of vitamins include vitamin A, vitamin C, vitamin E, vitamin D, vitamin K, and vitamin B group.

  As the sweetening agent other than stevia extract, saccharides or sweeteners can be used. Examples of the saccharide include sucrose, fructose, glucose, fructose-glucose liquid sugar, and reduced maltose. Examples of the sweetener include sugar, isomerized sugar, xylitol, palatinose, erythritol and the like, as well as high sweetness sweeteners such as aspartame, acesulfame potassium, neotame, saccharin, sucralose and the like. Moreover, sugar alcohols, such as sorbitol, may be included, and a sugarless bulk sweetener, a bulk sugar sweetener, etc. may be included.

  Examples of the acidulant include citric acid, trisodium citrate, adipic acid, gluconic acid, succinic acid, tartaric acid, lactic acid, fumaric acid, malic acid, and salts thereof, among which citric acid and citric acid Sodium, lactic acid, malic acid, tartaric acid, adipic acid and the like are preferable, and citric acid is particularly preferable.

  Examples of the fragrances include fragrances extracted from citrus and other fruits, fruit juices or fruit purees, plant seeds, rhizomes, bark, leaves and the like or extracts thereof, milk or dairy products, synthetic fragrances and the like.

  Examples of the mineral component include calcium, potassium, chromium, copper, fluorine, iodine, iron, magnesium, manganese, phosphorus, selenium, silicon, molybdenum, and zinc.

  Examples of the pigment component include carotenoid pigments such as marigold pigments, flavonoid pigments such as safflower pigments, anthocyanin pigments, gardenia pigments, betanine pigments such as beet pigments, chlorella, chlorophyll, etc. However, it is not particularly limited to these.

  Examples of the functional component include collagen, salmon cartilage, oyster extract, chitosan, propolis, octacosanol, tocopherol, carotene, polyphenol, plum extract, aloe, lactic acid bacteria, ganoderma, agaricus and the like.

  Moreover, the container-packed carbonated beverage according to the present embodiment may contain various esters, emulsifiers, preservatives, seasonings, gums, oils, pH adjusters, quality stabilizers, and the like.

  The pH of the container-packed carbonated beverage according to this embodiment is preferably 2 to 6, and more preferably 2 to 4. When the pH of the container-packed carbonated beverage is within the above range, a moderate acidity is obtained, and the beverage is preferably taste-preferred.

  The container used in the container-packed carbonated drink according to the present embodiment may be a commonly used drink container, but considering the gas pressure of carbon dioxide gas, plastic bottles and bottles such as metal cans and PET bottles It is preferable that the container has a predetermined strength. Moreover, in order to hold | maintain a carbon dioxide gas effectively after opening, it is preferable that the said container is equipped with the lid | cover which can be replugged.

  The container-packed carbonated beverage according to the present embodiment can be produced by a conventionally known method except that the above-described carbon dioxide gas retaining agent is blended. For example, the above-mentioned carbon dioxide holding agent is added to water, and the other components described above are further added as desired, followed by stirring to prepare a beverage stock solution. And after adjusting after pH adjustment and / or heat sterilization as needed, it can manufacture by the process of filling with carbon dioxide gas (carbonation), filling a container, and sterilizing. In addition, although there exist a premix method and a postmix method in the manufacturing method of carbonated drinks, any may be employ | adopted.

  The above carbon dioxide holding agent can effectively hold carbon dioxide when blended in a packaged carbonated beverage. In addition, the above-mentioned carbonated carbonated beverages are excellent in holding carbon dioxide gas. For example, even when left in a non-refrigerated state after opening the bottle, the carbonic acid gas is excellent in holding carbon dioxide. It is what is done.

  The embodiment described above is described for facilitating understanding of the present invention, and is not described for limiting the present invention. Therefore, each element disclosed in the above embodiment is intended to include all design changes and equivalents belonging to the technical scope of the present invention.

  Hereinafter, the present invention will be described in more detail by showing test examples and the like, but the present invention is not limited to the following test examples and the like. In addition, theafuran 90S (made by ITO EN) was used as a commercial item containing non-polymer catechins. Table 1 shows the contents of non-polymer catechins and the like contained in Theafuran 90S, and the respective contents of eight catechin compounds in the whole non-polymer catechins.

[Manufacture of carbonated soft drinks (1)]
An aqueous citric acid solution dissolved in pure water at room temperature and epigallocatechin gallate (EGCg) dissolved in pure water or warm pure water at 50 ° C. so that the final concentration in the packaged carbonated beverage becomes the concentration shown in Table 2. The mixture was mixed with an aqueous solution of the non-polymer catechins (Itoen Co., Ltd., Teafuran 90S) contained therein, sterilized at 98 ° C. for about 5 seconds, and then cooled to 5 ° C. The resulting beverage stock solution is made up to a specified amount with non-added carbonated water so that the carbon dioxide volume after post-sterilization is about 2.1, and the filling amount is 280 g in a 280 mL PET bottle that has been cleaned and sterilized. Weighed and filled so that Thereafter, sterilization was performed after securing a cold spot at 65 ° C. for 10 minutes to obtain a carbonated carbonated beverage (samples 1 and 2).

  Further, a citric acid aqueous solution dissolved in pure water at room temperature and a commercially available black oolong tea (manufactured by Suntory Ltd., oolong tea polymerized polyphenol display value: 70 mg / kg) so that the final concentration in the packaged carbonated beverage becomes the concentration shown in Table 2. 350 mL), and then sterilized at 98 ° C. for about 5 seconds, and then cooled to 5 ° C. The resulting beverage stock solution is made up to a specified amount with non-added carbonated water so that the carbon dioxide volume after post-sterilization is about 2.1, and the filling amount is 280 g in a 280 mL PET bottle that has been cleaned and sterilized. Weighed and filled so that Thereafter, sterilization was performed after securing a cold spot at 65 ° C. for 10 minutes to obtain a container-packed carbonated beverage (sample 3).

<Test Example 1> Determination of catechins, (−)-epigallocatechin gallate (EGCg), caffeine and polymerized catechin
In accordance with the method of Goto et al. (T. Goto, Y. Yoshida, M. Kiso and H. Nagashima, Journal of Chromatography A, 749 (1996) 295-299), catechins and caffeine by high performance liquid chromatography. Was quantified. In addition, based on the method described in Patent Document 1, polymerized catechin was quantified by high performance liquid chromatography.

<Test Example 2> Measurement of carbon dioxide volume The amount of carbon dioxide was measured as follows in accordance with the inspection method based on the JAS method. After putting each container-packed carbonated beverage (sample) of Samples 1 to 3 in a constant temperature water bath for 30 minutes or more and allowing to adjust to 20 ° C., gently take out the sample, attach a gas internal pressure gauge, and cap with a needle tip , Open the stopcock once, vent the gas (hereinafter referred to as “Snift”), immediately close the stopcock and shake vigorously to read the value (MPa) when the gauge pointer reaches a certain position Recorded.

  After sniffing, the gas internal pressure gauge was removed, the bottle was opened, and the liquid temperature was measured and recorded with a thermometer. The gas internal pressure and the liquid temperature obtained by the measurement were applied to the carbon dioxide absorption coefficient table, the required gas internal pressure was corrected for temperature, and the carbon dioxide volume was derived. The results are shown in Table 2.

<Test Example 3> Measurement of carbon dioxide residual rate After measuring the mass of each of the sampled carbonated beverages (samples) of Samples 1 to 3 (before opening), the sample was opened at room temperature (about 16 ° C). Each opened sample was allowed to stand at room temperature, re-plugged after 3 hours and 72 hours, and the mass of each sample was measured. The difference between the mass of the sample before opening and the mass of the sample after 72 hours considered to have completely lost carbon dioxide was calculated as the total amount of carbon dioxide contained in the sample. Further, the difference between the sample mass before opening and the sample mass after 3 hours of opening was calculated as the amount of carbon dioxide released after 3 hours of opening. From the total amount of carbon dioxide and the amount of carbon dioxide released 3 hours after opening, the carbon dioxide remaining rate after 3 hours from opening was calculated. The test was carried out in quadruplicate for each sample, and the average value was calculated. The results are shown in Table 2.

  As is apparent from Table 2, in a container-packed carbonated beverage having a carbon dioxide gas volume of 2.1 to 2.2, the residual rate of carbon dioxide gas after 3 hours after opening is significantly increased by adding catechin to the beverage. Increased, that is, outgassing of carbon dioxide after opening was suppressed (Samples 1 to 3). This effect was more remarkable in sample 2 than in sample 3. In Sample 2 and Sample 3, a significant difference was observed in the content of epigallocatechin gallate (EGCg) and the content of caffeine. From these results, it is considered that in sample 2, non-polymer catechins such as EGCg contained in sample 2 function as a carbon dioxide gas retention agent.

[Manufacture of packaged carbonated beverages (2)]
The citric acid aqueous solution described above and pure water or the aqueous solution of non-polymer catechins described above are mixed so that the final concentration in the packaged carbonated beverage becomes the concentration shown in Table 3, and the carbon dioxide gas volume is about 2 respectively. .6 (samples 4 and 5), about 2.9 (samples 6 and 7), and about 3.0 (samples 8 and 9), except that no added carbonated water was added, and the same as samples 1 and 2. Manufactured to obtain a packaged carbonated beverage (samples 4 to 9).

  For each of the container-packed carbonated beverages (samples) of Samples 4 to 9, the carbon dioxide volume and the carbon dioxide residual rate were measured in the same manner as in Test Examples 2 and 3 described above. The results are shown in Table 3.

  From the results of Table 3, it is shown that when the carbon dioxide gas volume is 2.6 to 2.7, outgassing of carbon dioxide gas is suppressed in the packaged carbonated beverage containing non-polymer catechins. (Samples 4 and 5). On the other hand, when the carbon dioxide volume is 2.9 to 3.0, it has been clarified that when non-polymer catechins are blended in a packaged carbonated beverage, the escape of carbon dioxide gas is promoted (Sample 6). ~ 9). From these results, it is considered that in sample 5, non-polymer catechins such as EGCg contained in sample 5 function as a carbon dioxide gas retaining agent.

[Manufacture of packed carbonated beverages (3)]
The citric acid aqueous solution described above is mixed with the above-described citric acid aqueous solution and the above-described aqueous solution of non-polymer catechins so that the final concentration in the packaged carbonated beverage becomes the concentration shown in Table 4, and the carbon dioxide gas volume is about 3. 3 was produced in the same manner as Samples 1 and 2 except that non-added carbonated water was added so as to be 3, and a carbonated carbonated beverage was obtained (Samples 10 and 11).

  For each of the container-packed carbonated drinks (samples) of Samples 10 and 11, the carbon dioxide volume and the carbon dioxide residual rate were measured in the same manner as in Test Examples 2 and 3 described above. The results are shown in Table 4.

  From the results of Table 4, it is shown that when the carbon dioxide volume is 3.2 to 3.3, outgassing of carbon dioxide gas is suppressed in a packaged carbonated beverage containing non-polymer catechins. (Samples 10 and 11). From these results, it is considered that in sample 11, the non-polymer catechins contained in sample 11 function as a carbon dioxide gas retention agent.

  Therefore, when the results in Tables 2 to 4 are combined, when the carbon dioxide gas volume is 2.85 or less, or 3.15 or more, carbon dioxide gas is released by adding non-polymer catechins. On the other hand, when the carbon dioxide volume was more than 2.85 and less than 3.15, it was revealed that the outgassing of carbon dioxide gas was promoted by adding non-polymer catechins.

[Manufacture of packaged carbonated beverages (4)]
The citric acid aqueous solution described above and the pure water and / or the aqueous solution of non-polymer catechins described above are mixed so that the final concentration in the packaged carbonated beverage becomes the concentration shown in Table 5, and the carbon dioxide volume is about Except having added non-added carbonated water so that it might be set to 2.5, it manufactured similarly to the samples 1 and 2, and the container-packed carbonated drink was obtained (samples 12-15).

  For each of the container-packed carbonated beverages (samples) of Samples 12 to 15, the carbon dioxide volume and the carbon dioxide residual rate (after 3 hours and 6 hours after opening) were measured in the same manner as in Test Examples 2 and 3 described above. did. The results are shown in Table 5.

  As shown in Table 5, in samples 13 to 15 containing non-polymer catechins, it became clear that outgassing of carbon dioxide gas was suppressed. From these results, it is considered that in samples 13 to 15, non-polymer catechins contained in each sample function as a carbon dioxide gas retention agent.

[Manufacture of packaged carbonated beverages (5)]
The aforementioned citric acid aqueous solution and the aforementioned non-polymer catechin aqueous solution are mixed so that the final concentration in the packaged carbonated beverage becomes the concentration shown in Table 6, and the carbon dioxide gas volume is about 2.5. Production was performed in the same manner as Samples 1 and 2 except that no additive carbonated water was added, and container-packed carbonated beverages were obtained (Samples 16 and 17).

<Test Example 4> Sensory evaluation A sensory evaluation test was performed on the obtained samples 16 and 17 and the above-described container-packed carbonated drinks (samples) of Samples 12 to 15. The sensory evaluation test was performed by tasting a 50 mL sample stored at 5 ° C. by seven trained panelists in charge of beverage development. The following criteria evaluated the astringency derived from catechin in five stages. Table 6 shows the average score of each panel. Moreover, from the evaluation result of astringency, it was determined whether it is preferable as a carbonated beverage according to the following criteria. The results are shown in Table 6.

= Evaluation of astringency =
1: I don't feel 2: I feel slightly 3: Weak 4: Strong 5: Extremely strong

= Judgment of astringency =
○: Preferred in preference (score of astringency is less than 3.5)
Δ: Slightly difficult, but generally good (Astringency score of 3.5 or more and less than 4.5)
×: unsuitable for taste (score for astringency is 4.5 or more)

  As shown in Table 6, when the content of the non-polymer catechins in the carbonated carbonated beverage is 0.040% by mass, the astringency derived from the catechin comes to be felt, and the carbonated beverage that is not preferred in preference. (Sample 15). Therefore, when the above-mentioned carbon dioxide gas retaining agent is blended in a container-packed carbonated beverage, it is considered preferable to blend so that the content of non-polymer catechins is 0.035% by mass or less.

[Manufacture of container-packed carbonated beverages (6)]
Stevia extract (Morita Chemical Industries, Rebaudio J-100, Rebaudioside A content: 96% by mass) and / or citric acid so that the final concentration in the carbonated carbonated beverage becomes the concentration shown in Table 7. Except that an aqueous solution in which pure water is dissolved in pure water and an aqueous solution of the above-mentioned non-polymer catechins are mixed and non-added carbonated water is added so that the carbon dioxide gas volume is about 2.7. Manufactured in the same manner as Samples 1 and 2, and container-packed carbonated drinks were obtained (Samples 18 to 25).

  For each of the sampled carbonated beverages (samples) of Samples 18 to 25, the carbon dioxide volume and the carbon dioxide residual rate were measured in the same manner as in Test Examples 2 and 3 described above. The results are shown in Table 7.

<Test Example 5> Sensory evaluation A sensory evaluation test was performed on each container-packed carbonated beverage (sample) of Samples 18-25. The sensory evaluation test was performed by tasting a 50 mL sample stored at 5 ° C. by seven trained panelists in charge of beverage development. The astringency derived from catechin was evaluated in 5 stages according to the same criteria as in Test Example 4 described above, and the badness of sweetness derived from stevia extract was evaluated in 5 levels according to the following criteria. Table 7 shows the average score of each panel. In addition, comprehensive evaluation was made based on the results of the above two items. The results are shown in Table 7.

= Evaluation of post-sweetness =
1: There is no after-effect of sweetness 2: It feels a slight after-effect of sweetness 3: It feels after-effect of sweetness, but there is no problem in drinking Feel strongly

= Comprehensive evaluation =
○: Preferred in preference (all scores are less than 3.5)
Δ: Slightly difficult in preference, but generally good (any score is 3.5 or more and less than 4.5)
×: Unpreferable preference (any score is 4.5 or more)

  As shown in Table 7, when the carbon dioxide gas volume is 2.7 to 2.8, the carbonated beverage containing 0.010% by mass and 0.020% by mass of non-polymer catechins, respectively, The outgassing of the gas was suppressed (Samples 18, 19 and 23). Moreover, in the container-packed carbonated drink containing non-polymer catechins, the outgassing of carbon dioxide was further suppressed by further blending stevia extract (Samples 18 to 22, Samples 18 and 23 to 25). From these results, in samples 19 and 23, non-polymer catechins contained in each sample, and in samples 20 to 22 and 24 to 25, non-polymer catechins and rebaudioside A contained in each sample. It is considered that each stevia extract functions as a carbon dioxide gas retaining agent. However, when 0.06% by mass of stevia extract was blended, although the effect of suppressing gas loss was observed, the badness of sweetness derived from stevia extract was conspicuous, and the carbonated beverage became unpreferably unfavorable (sample) 22).

[Manufacture of packaged carbonated beverages (7)]
Mixing the above-mentioned stevia extract and / or citric acid aqueous solution with pure water or the above-mentioned non-polymer catechin aqueous solution so that the final concentration in the packaged carbonated beverage becomes the concentration shown in Table 8, and Manufactured in the same manner as Samples 1 and 2 except that non-added carbonated water was added so that the carbon dioxide volume was about 3.3 to obtain a container-packed carbonated beverage (Sample 26).

  About the container-packed carbonated beverage (sample) of the obtained sample 26, the carbon dioxide gas volume and the carbon dioxide gas residual ratio were measured in the same manner as in Test Examples 2 and 3 described above, and the same as in Test Examples 4 and 5 described above. Evaluation was performed. In addition, for the samples 10 and 11 described above, sensory evaluation was performed in the same manner as in Test Examples 4 and 5. The results are shown in Table 8.

  As shown in Table 8, when the carbon dioxide gas volume is 3.2 to 3.3, the outgassing of carbon dioxide gas is suppressed by blending non-polymer catechins, and further stevia extract is blended. As a result, outgassing of carbon dioxide was further suppressed (Samples 10, 11 and 26). From these results, in sample 26, it is considered that stevia extracts such as non-polymer catechins and rebaudioside A contained in sample 26 function as a carbon dioxide gas retention agent. In addition, each container-packed carbonated drink of Samples 10, 11 and 26 was a preferred carbonated drink with little astringency and no bad after-sweetness.

[Manufacture of packaged carbonated beverages (8)]
The above-mentioned stevia extract and / or aqueous solution of citric acid and pure water or an aqueous solution of non-polymer catechins are mixed and carbon dioxide gas so that the final concentration in the packaged carbonated beverage becomes the concentration shown in Table 9 A container-packed carbonated beverage was obtained in the same manner as Samples 1 and 2 except that non-added carbonated water was added so that the volume was about 3.0 (Samples 27 to 29).

  For each of the sampled carbonated beverages (samples) of Samples 27 to 29, the carbon dioxide volume and the carbon dioxide residual rate were measured in the same manner as in Test Examples 2 and 3 described above. The results are shown in Table 9.

  As shown in Table 9, when the carbon dioxide gas volume was 2.9 to 3.0, the outgassing of carbon dioxide gas was promoted in the packaged carbonated beverage containing non-polymer catechins (Sample 27 and Sample 27). 28). On the other hand, the inclusion of stevia extract in addition to non-polymer catechins not only suppresses the gas release promoting action by non-polymer catechins, but also does not contain non-polymer catechins and stevia extract. Compared with carbonated beverages, the gas escape of carbon dioxide could be suppressed (Samples 27 to 29). From these results, in sample 29, it is considered that stevia extracts such as non-polymer catechins and rebaudioside A contained in sample 29 function as a carbon dioxide gas retention agent.

  INDUSTRIAL APPLICABILITY The present invention is useful as a carbon dioxide gas retaining agent capable of effectively retaining carbon dioxide gas and a container-packed carbonated beverage in which the outflow of carbon dioxide gas is suppressed. In particular, according to the present invention, since carbon dioxide gas can be retained even after non-refrigeration after opening, the container-packed carbonated beverage of the present invention is often left uncooled after opening. It is suitable as a beverage.

Claims (9)

Containing non-polymer catechins as active ingredients ,
The non-polymer catechins contain epigallocatechin gallate, and the content of the epigallocatechin gallate in the non-polymer catechins is 60% by mass or more,
Furthermore, the ratio of the content [P] (mass%) of the polymerized catechin to the content [C] (mass%) of the non-polymer catechins when the polymer catechin is not contained or polymerized catechin is contained [ Carbon dioxide holding agent, wherein P] / [C] is 0.25 or less . Furthermore, it contains stevia extract as an active ingredient, and the ratio [S] / [C of the content [S] (mass%) of the stevia extract to the content [C] (mass%) of the non-polymer catechins carbon dioxide retention agent according to claim 1, characterized in that] it is 0.02 to 5. A container-packed carbonated beverage characterized by comprising the carbon dioxide gas retaining agent according to claim 1 . The container-packed carbonated drink according to claim 3 , wherein the gas volume of carbon dioxide is 1.8-2.85, or 3.15-4.0. A container-packed carbonated drink comprising the carbon dioxide gas retaining agent according to claim 2 . The container-packed carbonated drink according to claim 5 , wherein the gas volume of the carbon dioxide gas is 1.8 to 4.0. As the content of the non-polymer catechins in the packaged carbonated beverage is from 0.001 to 0.035 wt%, any claim 3-6, characterized in that blended with the carbon dioxide retaining agent The packaged carbonated drink according to claim 1. A method for retaining carbon dioxide in a container-packed carbonated beverage,
Including the non-polymer catechins as an active ingredient in the packaged carbonated beverage ,
The non-polymer catechins contain epigallocatechin gallate, and the epigallocatechin gallate is contained so that the content of the epigallocatechin gallate in the non-polymer catechins is 60% by mass or more. Let
In addition, the container-packed carbonated beverage does not contain polymerized catechins, or the polymerized catechin content [P] (mass%) relative to the content [C] (mass%) of the non-polymer catechins in the container-packed carbonated drink ) The non-polymer catechins and the polymerized catechins are contained so that the ratio [P] / [C] is 0.25 or less. . Furthermore, stevia extract is contained as an active ingredient, and the ratio [S] / [C of the content [S] (mass%) of the stevia extract to the content [C] (mass%) of the non-polymer catechins. ] The non-polymer catechins and the stevia extract are contained so that the ratio becomes 0.02 to 5. The method for retaining carbon dioxide in a carbonated carbonated beverage according to claim 8.