JP4761584B2 - Method for producing processed aloe mesophyll from which viscous substances have been removed - Google Patents

Description

  The present invention relates to a method for producing a processed aloe mesophyll from which viscous substances have been removed.

  Aloe is a general term for succulent plants belonging to the genus Aloe in the Aloe family, and more than 300 species are known in the world. In Japan, almond vera is mainly used for ornamental and edible purposes, and aloe vera is used for edible purposes. Since aloe is expected to have various medicines and health effects, many foods and drinks containing aloe are on the market against the background of the recent health boom. In particular, yogurt with aloe vera mesophyll has grown to a point where consumers can accept the refreshing texture and taste peculiar to aloe without bitterness and have been recognized as a long-life product.

  A general method for producing processed aloe mesophylls used in these foods and beverages is to cut raw aloe leaves, peel, wash, cut, blanch, wash, and then add syrup, etc. in some cases. The process consists of filling and sterilizing.

  When aloe mesophyll is cut, a transparent, slimy, jelly-like viscous substance leaches out from the cut surface, which is presumed to be acemannan (acetylated glucomannan), a kind of polysaccharide. ing. There are reports that this jelly-like viscous substance has the ability to stimulate the immune system, put a protective film, and accelerate healing of wounds when applied to the affected area of burns, wounds, and insect bites.

  However, the aloe mesophyll to which the above-mentioned viscous substance adheres is so-called that, when blended in food or drink, the good texture of the aloe mesophyll is impaired, and various foreign substances and microorganisms adhere to the viscous substance or enter the inside. It must be removed because it may cause contamination. Usually, after washing with a large amount of water, after passing through a blanching or citric acid dipping step, the viscous substance is removed by washing again, but the work is complicated and complete removal is not easy, which also causes high costs. Yes. The reason is that the viscous substance is the main component of aloe mesophyll, and even if the viscous substance on the cut surface is physically removed by washing with water, it gradually leaches out over time.

  So far, proposals for removing viscous substances from aloe vera flesh include aloe vera processed foods obtained by making noodles or tocorotene-like mesophylls with aloe vera leaf epidermis removed, boiled, and removing surface null nulls. There is a proposal of a manufacturing method (Patent Document 1). The above proposal is a method of hardening the viscous material on the mesophyll surface by blanching and making it easy to handle, but of course, there was a major drawback that the viscous material remained and the good texture of the aloe mesophyll was impaired. .

Japanese Patent No. 3982956

  The present invention relates to a method for producing a processed aloe mesophyll from which a viscous substance has been removed by a simple and efficient method.

  The present inventors have conducted various studies on an effective method for replacing the viscous substance removal method from aloe mesophyll by processes such as blanching and washing with water. However, washing with a large amount of water is not only disadvantageous from the viewpoint of cost and workability, but also has a serious disadvantage that the aloe mesophyll is damaged and the active ingredient polysaccharide is lost. The removal by the method was judged to be difficult and disadvantageous. Therefore, the present inventors considered that a method using an enzyme is effective for removing a viscous substance, and conducted intensive studies.

  As a result, aloe mesophyll was dipped in 0.0005 to 0.1% by weight aqueous solution of polysaccharide degrading enzyme such as mannanase for a short time and washed with water. It has been found that no viscous material leaches out and the present invention has been completed.

  Thus, the present invention provides a method for producing a processed aloe mesophyll, characterized in that a polysaccharide-degrading enzyme is allowed to act on aloe mesophyll to remove viscous substances.

  The present invention also provides the processed aloe mesophyll product, wherein the polysaccharide-degrading enzyme is a polysaccharide-degrading enzyme containing mannanase.

  Furthermore, the present invention provides a processed aloe medicinal product obtained by the above-described production method.

  According to the present invention, an aloe mesophyll processed product from which a viscous substance has been removed can be obtained by a simple operation, which can greatly improve workability and reduce manufacturing costs, and reduce the possibility of contamination. Can do. Moreover, the obtained aloe mesophyll product has better texture and less damage to mesophyll compared to conventional products, and foods and drinks containing this can further satisfy consumer preferences.

Hereinafter, the present invention will be described in more detail.
The aloe vera that can be used as a raw material in the method of the present invention may be any plant belonging to the genus Aloeaceae (Aloeae), but aloe vera having no bitter taste and good texture is preferred.

  The aloe mesophyll that can be used as a raw material in the method of the present invention is one in which the epidermis has been peeled off from the raw aloe leaf, or one in which the peeled aloe mesophyll has been cut into a suitable size, for example, 2 mm to 25 mm in a piece Alternatively, aloe mesophyll cut into an arbitrary shape can be used. Furthermore, you may use what heated said aloe mesophyll.

  Examples of polysaccharide-degrading enzymes that can be used in the present invention include enzymes that act on polysaccharides such as cellulase, hemicellulase, mannanase, xylanase, amylase, and pectinase to produce monosaccharides and oligosaccharides. However, it is not limited to these. Of these polysaccharide-degrading enzymes, an enzyme containing mannanase is particularly preferred.

  Commercially available enzymes include, for example, mannanase BGM “Amano”, hemicellulase “Amano” 90, cellulase A “Amano” 3, pectinase PL “Amano” (above, manufactured by Amano Enzyme); β-1,4-mannanase (Yakult) Kyristase P3 (manufactured by Daiwa Kasei Co., Ltd., registered trademark); Sumiteam ACH, Sumiteam AC, Sumiteam X, Sumiteam SPC (above, Shin Nippon Chemical Co., Ltd., registered trademark); Cellulosin GM5 (manufactured by HIBI) , Registered trademark), etc., but is not limited thereto.

  Among these commercially available enzymes, as the polysaccharide-degrading enzyme containing mannanase, among the polysaccharide-degrading enzymes, mannanase BGM “Amano”, Sumiteam ACH, Sumiteam AC, cellulosin GM5, β-1,4-mannanase However, it is not limited to these.

  The enzyme concentration of the polysaccharide-degrading enzyme is a concentration in the range of 0.0005 to 0.1% by weight with respect to water, preferably a concentration in the range of 0.001 to 0.01. . Further, the reaction pH of the enzyme may be a pH within the range of pH 3 to 7, preferably a pH within the range of pH 4.0 to 6.0. Furthermore, the optimum temperature of the enzyme can include a temperature within a range of 10 to 70 ° C. depending on the type of the enzyme, but the actual reaction temperature is preferably 15 to 45 ° C. in order to maintain the quality of aloe. A temperature within the range, more preferably a temperature within the range of 25 to 35 ° C can be mentioned.

  As a method of actually causing the polysaccharide-degrading enzyme to act on the aloe mesophyll, for example, it can be carried out by immersing the aloe mesophyll in an aqueous solution of the polysaccharide-degrading enzyme for about 10 seconds to 30 minutes. Since the immersion time varies depending on the type, concentration, and reaction conditions of the enzyme, appropriate immersion is performed by combining these. Further, as a method other than immersion, an aqueous solution of polysaccharide-degrading enzyme may be showered on the aloe mesophyll so that the aqueous solution of polysaccharide-degrading enzyme is spread on the surface of aloe mesophyll.

  If the enzyme concentration exceeds the above range or the contact time of the aloe mesophyll with the enzyme aqueous solution becomes longer, the surface of the aloe mesophyll becomes harder, so the enzyme concentration and the contact time for the aqueous enzyme solution should be adjusted so that they are within the desired hardness range. It is necessary to select appropriately.

  After allowing the polysaccharide-degrading enzyme to act on the aloe mesophyll, the viscous substance is removed from the aloe mesophyll by washing with a shower or the like according to a conventional method. If the degree of washing is insufficient, viscous substances will remain and cause contamination by foreign substances such as dust and microorganisms.

  The obtained processed aloe mesophyll can be transported and stored by refrigeration after filling into a plastic bag or other container as it is. Alternatively, the obtained aloe mesophyll product can be made into a processed aloe mesophyll product that has been dipped in syrup containing sugars, acids, stabilizers, and the like. At that time, if desired, any food material or additive such as natural extract, perfume, antioxidant, pigment and vitamin can be added.

Thus, according to the present invention, it is possible to provide an aloe mesophyll processed product from which viscous substances are removed and which is less contaminated with foreign matter. Moreover, the workability is remarkably improved and the cost can be reduced by removing the viscous substance by the polysaccharide-degrading enzyme. Furthermore, by adding the processed aloe mesophyll of the present invention to various foods and drinks, for example, yogurt, taste drinks, jelly products, etc., food and drink with good texture and high taste can be provided.
Hereinafter, the present invention will be described more specifically with reference to Examples and Comparative Examples.

Example 1
After harvesting the fresh leaves of aloe vera, the surface was washed, and the epidermis was peeled off to obtain an internal gel-like mesophyll. Next, the mesophyll was immersed in a 0.002% by weight aqueous solution of mannanase BGM “Amano” for 30 seconds. After soaking, washing with a mesophyll washer was performed to remove impurities adhering to the mesophyll. The mesophyll after the removal of contaminants was cut into 5 mm × 5 mm with a dicer, washed with water with a mesophyll washing machine, then drained and sorted, and further fine impurities and foreign matters were removed. The selected mesophyll was filled in a plastic bag to obtain a processed aloe mesophyll (Product 1 of the present invention).

Example 2
After harvesting the fresh leaves of aloe vera, the surface was washed, and the epidermis was peeled off to obtain an internal gel-like mesophyll. Next, the mesophyll was immersed in a 0.002% by weight aqueous solution of mannanase BGM “Amano” for 30 seconds. After soaking, washing with a mesophyll washer was performed to remove impurities adhering to the mesophyll. The mesophyll after removal of impurities was cut into 5 mm × 5 mm with a dicer. The cut aloe mesophyll was again immersed in a 0.002 wt% aqueous solution of mannanase BGM “Amano” for 30 seconds. The mesophyll after immersion was washed with a mesophyll washing machine, then drained and sorted, and fine impurities and foreign matters were removed. The mesophyll after the selection was filled into a plastic bag to obtain a processed aloe mesophyll (Product 2 of the present invention).

Example 3
The same operation as in Example 1 was performed except that the cut size of the dicer was changed to 10 mm × 10 mm in Example 1 to obtain an aloe mesophyll processed product (present product 3).

Example 4
The same operation as in Example 2 was performed except that the cut size of the dicer was changed to 10 mm × 10 mm in Example 2 to obtain an aloe mesophyll processed product (present product 4).

Example 5
The same operation as in Example 1 was performed except that the cut size of the dicer was changed to 12 mm × 12 mm in Example 1 to obtain an aloe mesophyll processed product (Product 5 of the present invention).

Example 6
The same operation as in Example 2 was performed except that the cut size of the dicer was changed to 12 mm × 12 mm in Example 2 to obtain an aloe mesophyll processed product (Invention product 6).

Examples 7-12
An aloe mesophyll processed product was prepared in the same manner as in Examples 1 to 6, except that 0.005 wt% aqueous solution of Mannanase BGM “Amano” in Examples 1 to 6 was used instead of 0.005 wt% aqueous solution of Sumiteam ACH. (Invention products 7 to 12) were obtained.

Examples 13-18
An aloe mesophyll processed product was prepared in the same manner as in Examples 1 to 6, except that 0.005 wt% aqueous solution of Sumiteam AC was used instead of the 0.002 wt% aqueous solution of mannanase BGM "Amano" of Examples 1-6. (Invention products 13 to 18) were obtained.

Examples 19-24
An aloe mesophyll processed product was prepared in the same manner as in Examples 1 to 6, except that a 0.005 wt% aqueous solution of cellulosin GM was used instead of the 0.002 wt% aqueous solution of mannanase BGM "Amano" of Examples 1-6. (Invention products 19 to 24) were obtained.

Comparative Example 1
After harvesting the fresh leaves of aloe vera, the surface was washed, and the epidermis was peeled off to obtain an internal gel-like mesophyll. The mesophyll was washed in a water tank, and then washed with a mesophyll washing machine to remove impurities attached to the mesophyll. The mesophyll after removal of impurities was cut into 5 mm × 5 mm with a dicer. The mesophyll after cutting was immersed in the first water tank for 20 minutes, transferred to the second water tank, immersed for 20 minutes, further transferred to the third water tank, immersed for 20 minutes, washed with shower water, and then washed with 0.0. It was immersed in a 05 wt% citric acid solution for 30 minutes. After soaking, the flesh washer was washed with water, drained and sorted, and fine impurities and foreign matters were removed. The sorted mesophyll was filled into a plastic bag to obtain a processed aloe mesophyll (Comparative Product 1).

Comparative Example 2
After harvesting the fresh leaves of aloe vera, the surface was washed, and the epidermis was peeled off to obtain an internal gel-like mesophyll. The mesophyll was washed in a water tank, and then washed with a mesophyll washing machine to remove impurities attached to the mesophyll. The mesophyll after removal of impurities was cut into 5 mm × 5 mm with a dicer. The mesophyll after cutting is immersed in the first water tank for 20 minutes, transferred to the second water tank, immersed for 20 minutes, further transferred to the third water tank, immersed for 20 minutes, washed with shower water, and heated to 90 ° C. in warm water. Soaked for 30 seconds. After soaking, the flesh washer was washed with water, drained and sorted, and fine impurities and foreign matters were removed. The sorted mesophyll was filled in a plastic bag to obtain a processed aloe mesophyll (comparative product 2).

Comparative Examples 3 and 4
The same operations as in Comparative Examples 1 and 2 were performed except that the cut size of the dicer was changed to 10 mm × 10 mm in Comparative Examples 1 and 2, and processed aloe mesophyll products (Comparative products 3 and 4) were obtained.

Comparative Examples 5 and 6
The same operations as in Comparative Examples 1 and 2 were performed except that the cut size of the dicer was set to 12 mm × 12 mm in Comparative Examples 1 and 2, and processed aloe mesophyll products (Comparative products 5 and 6) were obtained.
(Evaluation results)
Table 1 shows the observation results of the state of the mesophyll surface after treatment of the inventive products 1 to 24 and comparative products 1 to 6.

  Of the inventive products 1 to 23, 12 odd-numbered products are obtained by enzymatically treating peeled aloe mesophyll, washing with water, cutting and then washing with water again. As shown in the observation results shown in Table 1, the viscous substances on the surface were removed. However, it was confirmed that the viscous material exudes slightly when pressed strongly.

  On the other hand, of the present invention products 2 to 24, the even numbered 12 products are obtained by enzymatically treating aloe mesophyll with water and then cutting it with a dicer, followed by enzymatic treatment and washing with water again. In any of these, the viscous material on the surface was removed, and no exudation of the viscous material was observed even when pressed strongly. Further, when the hardness of the aloe mesophyll was confirmed sensorially, it was confirmed that the hardness increased each time by performing the first and second enzyme treatments as compared to before the enzyme treatment.

  The difference in the amount of enzyme added is presumed to be due to the strength of the activity of the enzyme used or the type, composition, etc. of the enzyme.

On the other hand, the comparative products 1 to 6 treated with the citric acid solution and the hot water at 90 ° C. had a viscous substance remaining on the surface, and when pressed, the viscous substance oozed out and was not sufficiently removed. Further, since the viscous material does not remain, foreign matters such as fine dust adhering to the surface or entering the inside cannot be completely removed.
In addition, the products 1 to 24 of the present invention were refrigerated and stored at 10 ° C. for 3 weeks, and the viscous substances were not leached and the texture of the aloe mesophyll was also good.
Also, from the viewpoint of work, it was confirmed that the work time can be shortened and the cost can be reduced.

Claims (2)

  A method for producing a processed aloe mesophyll, characterized by removing a viscous substance from aloe mesophyll by allowing a polysaccharide-degrading enzyme to act on aloe mesophyll and then washing with water. The method for producing a processed aloe mesophyll according to claim 1, wherein the polysaccharide-degrading enzyme is a polysaccharide-degrading enzyme containing mannanase.