JP4634062B2 - Biological hold kit and storage container - Google Patents

Description

  The present invention relates to a biological hold kit used for storing and transporting Escherichia coli, phage, DNA, cells, and the like, and a storage container thereof.

  Conventionally, substances produced by microorganisms have been used as a source of new drug development, and microorganisms producing such useful products have been searched.

  In addition, with the recent development of genetic recombination technology, the target DNA can be produced in large quantities by transforming useful substance DNA (RNA) into Escherichia coli, phage, or the like and propagating it. In addition, a method of amplifying DNA in a test tube by a PCR method is also performed.

  When conducting such an experiment, a multi-well plate in which a plurality of wells are regularly arranged is used. By using a multi-well plate, a large number of samples can be handled with one plate, and the efficiency of experiments can be improved.

  FIG. 4 shows an example of a multiwell plate. FIG. 4A is a plan view, and FIG. 4B is a cross-sectional view taken along the line A-A ′. The multiwell plate 400 is provided with 96 wells 401 in a total of 12 columns and 8 rows. A culture solution is injected into each well 401 of the multi-well plate 400, and Escherichia coli, DNA, or the like is added to each well 401 and cultured.

  At present, a great variety of microorganisms such as Escherichia coli and samples such as DNA are stored, amplified and used for examination and treatment. The preservation method is usually a puncture culture method, a freezing method, a freeze-drying method, or the like. A research institution or the like that stores stored microorganisms is transported and supplied while preserving the microorganisms or the like in response to requests from other research institutions.

  As a method for storing and transporting microorganisms and the like, conventionally, a method is generally used in which a fixed amount of filter paper is placed on a petri dish and a sample is soaked in the filter paper and stored.

  However, according to this method, microorganisms are bulky at the time of storage and transportation, and in order to send them in large quantities, the transportation cost is great and a large storage place is required. Even in the case of amplification, there was a problem that microorganisms taken out from the petri dish were taken out one by one and planted in each well of the multi-well plate.

  In addition, DNA was stored in a filter paper soaked and dried. However, it is necessary to cut the filter paper and immerse it in the culture solution of each well of the multiwell plate every time it is used. The filter paper had to be removed, and very complicated work was required.

  In order to solve such problems, in the following patent application, a synthetic resin sheet is punched between the upper and lower two sheets at positions corresponding to each well of the multi-well plate, and both sides of the synthetic resin sheet are adhesive. A thin sheet-like storage / conveying container has been developed which is used by adhering a sheet of paper with a filter paper placed on the punched portion.

The sheet-like storage / transport container is an excellent storage / transport container that is thin, is not bulky, does not break, and can supply a sample to each well of the multi-well plate at one time.
Japanese Patent Application No. 2003-359534

  However, since the above-mentioned sheet-like storage / conveying container is used by sticking the adhesive sheet with filter paper attached to the multi-well plate, it is easy to cause contamination of germs in the poorly adhered area, and the adhesive sheet is peeled off from the multi-well plate. Occasionally, there is a problem that turbidity is likely to occur due to liquid dripping in adjacent wells. In addition, it is disposable, cannot be used repeatedly, and there is a problem that valuable specimens are wasted. Furthermore, since it is sealed in a thin sheet, there is a problem that the amount of air is small and the specimen E. coli cannot live for a long time.

  The present invention has been made in view of the above circumstances, can be efficiently stored and transported, can reliably supply a specimen to each well of a multi-well plate, and can survive microorganisms for a long period of time, And it aims at providing the biological body hold kit which can be used repeatedly.

  Another object of the present invention is to provide a storage container that can seal and store and transport such a biological hold kit.

  In order to achieve the above object, the present invention provides a plate-like portion disposed on the upper surface of a multi-well plate in which a plurality of wells are arranged, and a position corresponding to each well of the plate-like portion. There is provided a living body holding kit comprising: a protruding portion that is provided so as to be inserted into the protruding portion; and a water absorbing member that is held at the protruding end portion of the protruding portion.

  In this method of using the living body hold kit, after performing an operation for allowing the specimen to infiltrate into the water-absorbing member held at the protruding end of each protrusion, the sample is stored in a storage container and transported. When in use, remove from the storage container and place the plate-shaped part on the upper surface of the multi-well plate so that the protruding part is on the bottom. Each protruding part enters into each well of the multi-well plate, and the protruding end of the protruding part. The water-absorbing member held in the section is immersed in the culture solution in the well, and the specimen can be supplied to each well.

  This living body hold kit can store and transport many types of samples corresponding to the wells of the multi-well plate, and can supply the samples to each well of the multi-well plate in a single operation. . Therefore, the storage / transport efficiency is very good. Moreover, since the water-absorbing member held by the independent protrusions is taken out after being immersed in the culture solution in the well, turbidity between adjacent wells hardly occurs. Moreover, since the specimen can be supplied once and then stored again in the storage container, it can be used repeatedly. Furthermore, the water-absorbing member has a shape that is held at the protruding end of the protruding portion, and since a gap is created between the protruding portion and the container that houses the protruding portion, microorganisms can survive for a long time with the air in the gap. It is.

  In addition, the present invention provides the living body hold kit according to claim 1, wherein the protrusion has a hollow portion in which an opening is formed in the plate-like portion.

  By making the projection part into a hollow shape in which an opening is formed in a plate-like part, the specimen can be supplied from the upper side with the projection part facing down. Thus, the specimen can be supplied in the same multiple pattern as the multiwell plate.

  In addition, the present invention provides the living body hold kit according to claim 2, wherein a through hole is provided in a protruding end portion of the projecting portion having the hollow portion.

  This through-hole functions as a passage for supplying the specimen to the water absorbing member held outside the protruding end portion of the protruding portion.

  The present invention also provides a plate-like portion disposed on the upper surface of a multi-well plate in which a plurality of wells are arranged, and the plate-like portion is inserted into the well provided at a position corresponding to the well. A storage container for a living body hold kit having a protruding portion protruding as described above and a water absorbing member held at the protruding end of the protruding portion, the flat plate portion on which the plate-like portion is placed, and A living body hold having a storage container body having a well for receiving each of the protrusions provided at a position corresponding to the protrusion of the flat plate portion, and a lid portion fixed to the flat plate portion. Provided is a storage container in which each water-absorbing member is enclosed in each of the storage wells when the kit is stored.

  The storage container is used by placing the plate-like portion of the living body hold kit of the present invention on the flat plate portion of the storage container main body and fixing the lid portion to the flat plate portion so that each protrusion is provided in each well of the storage container. Each is enclosed and contained. Thereby, each water-absorbing member of the living body hold kit can be sealed and isolated and stored and transported.

  The biological hold kit of the present invention can be efficiently stored and transported, can reliably supply a specimen to each well of a multi-well plate, can survive microorganisms for a long period of time, and can be used repeatedly. Is possible.

  In addition, the storage container of the present invention can be reliably stored and transported by sealing the living body hold kit of the present invention.

  Hereinafter, embodiments of the living body hold kit and the storage container of the present invention will be described, but the present invention is not limited to the following embodiments.

  FIG. 1 shows one embodiment of the living body hold kit of the present invention, FIG. 1 (a) is a plan view, and FIG. 1 (b) is a side view.

  This living body hold kit 1 has a kit body 2 formed by integral molding of plastic, and the kit body 2 has a plate-like portion 3 that fits on the upper surface of the multi-well plate and each well of the multi-well plate. It has a plurality of protrusions 4 protruding from the plate-like part 3 to be accommodated, and has a water absorbing member 5 held at the protruding end of each protrusion 4.

  The size and shape of the multi-well plate are almost uniform worldwide, and the shape is the same. However, depending on the size of the well, 4 well, 12 well, 24 well, 48 well, 96 well, 384 well, etc. There are variations, depending on the purpose of use.

  The multi-well plate 400 shown in FIG. 4 has 96 wells provided with a total of 96 wells 401 in 12 rows and 8 rows. It is integrally molded with polypropylene, and can withstand the rise and fall of temperature for a short time in the PCR method. Some multi-well plates are formed of transparent polystyrene having a flat bottom surface. The peripheral edge of the opening of each well 401 of the multi-well plate 400 has a rib shape protruding slightly from the upper surface. Further, an outer peripheral rib 402 protruding from the upper surface is provided at the periphery of the upper surface of the multiwell plate. In addition, a notch 403 for positioning is provided at one corner of the multi-well plate 400.

  The living body hold kit 1 of the present invention can be applied to any multiwell plate. Hereinafter, a biological hold kit suitable for the 96-well multi-well plate 400 shown in FIG. 4 will be described.

  The plate-like portion 3 constituting the kit body 2 has a shape in which the outer edge is substantially in contact with the inner surface of the outer peripheral rib 402 provided on the peripheral edge of the upper surface of the multi-well plate 400 and is placed on the upper surface of the multi-well plate 400. There is a notch 31 corresponding to a notch 403 for positioning provided at one corner of the multi-well plate 400.

  The projecting portions 4 constituting the kit body 2 are formed integrally with the plate-like portion 3 and are provided so as to protrude from the plate-like portion 3 at positions where the centers of the wells of the multi-well plate 400 coincide with the centers. Yes. When used for a 96-well multi-well plate, the wells are formed at a pitch of 9 mm, and thus the protrusions 4 are formed at the same pitch.

  FIG. 2A shows an example of the protrusion 4 and an enlarged cross-sectional view of the water absorbent member 5 along the line B-B ′ of FIG. The projecting portion 4 has a tapered truncated cone shape that becomes thinner from the base end side toward the projecting end side, and has a hollow portion 42 that communicates with an opening 41 provided in the plate-like portion 3, and becomes hollow. ing. The protruding end of the protrusion 4 has a circular flat bottom surface. A through hole 43 for infiltrating the chemical solution is formed in the center of the bottom surface of the tip. A disc-shaped water-absorbing member 5 having the same diameter as the bottom surface of the protrusion 4 is fixed to the protruding end of the protrusion 4 with an adhesive. A rectangular air vent hole 44 extending vertically is formed in the wall surface of the protrusion 4. The lower end of the air vent hole 44 is preferably higher than the inner bottom surface of the protrusion 4 by about several mm. The outer diameter of the base end portion of the protrusion 4 is preferably slightly smaller than the inner diameter of the open end of the well 401 of the multi-well plate 400.

  The protrusion height of the protrusion 4 is such that the water-absorbing member 5 held at the protrusion end of the protrusion 4 enters the well 401 of the multi-well plate 400 and is immersed in the culture solution injected into the well 401. As described above, it is preferable that more than half can enter the well 401 of the multi-well plate 400. As shown in FIG. 4B, each protrusion 4 of the biological hold kit 1 indicated by a broken line is inserted into each well 401 of the multi-well plate 400, and the plate-like portion 3 of the biological hold kit 1 is moved to the multi-well. When placed on the upper surface of the plate 400, the lower surface of the plate-like portion 3 of the biological hold kit 1 is on the rib at the periphery of the opening of the well 401 of the multi-well plate 400, and the periphery of the plate-like portion 3 is the multi-well plate 400. It will be in the state accommodated in the inner surface of the outer periphery rib 402 of this. At this time, it is preferable to set the height of the protruding portion 4 so that the water absorbing member 5 at the protruding end portion of the protruding portion 4 is located slightly below the middle of the well 401.

  The water-absorbing member 5 may have any shape and material as long as a liquid containing microorganisms or DNA can permeate and hold in order to hold a specimen that is a target object for storage and transport. As the material, fibrous materials such as filter paper, non-woven fabric, felt, and cellulose (including nitrocellulose) are used. The shape is preferably a disk shape, a spherical shape, a hemispherical shape, or the like. As a method of holding the water absorbing member 5 on the protruding end portion of the protruding portion 4, it is preferable to use an adhesive. As the adhesive, a silicone-based adhesive is preferable, and an adhesive that has been recognized as having no abnormality in a mutagenicity test as to whether or not to induce mutation is used. Further, a holding member that sandwiches and holds the water absorbing member 5 at the protruding end portion of the protruding portion 4 may be provided.

  Such a biological hold kit 1 is manufactured by, for example, forming the kit body 2 by integrally forming the plate-like portion 3 and the protruding portion 4 from a plastic sheet by a vacuum forming method or a pressure forming method. It is possible to employ a method in which a through hole 43 at the projecting end and an air vent hole 44 at the side surface are formed, and the water absorbing member 5 cut out in a predetermined shape is attached to the projecting end surface of the projecting portion 4 with an adhesive. Examples of the material for the plastic sheet include polyolefin resins such as polyethylene and polypropylene, polyester resins such as polyethylene terephthalate and polybutylene terephthalate, and polyamide resins. In general, polypropylene can be preferably used. Although the thickness of a plastic sheet is selected suitably, it can be set as the range of 0.1-1 mm.

  In the above description, the protruding portion 4 is described as being hollow, but as shown in FIG. 2B, it may be a truncated cone-shaped columnar solid protruding portion 4b. An injection molding method is suitable for integrally molding the protruding portion 4b and the plate-like portion 3 as described above.

  Further, as shown in FIG. 2 (c), the water absorbing member 5 held at the protruding end portion of the protruding portion 4 can be arranged so as to be held on the inner bottom surface of the hollow portion 42 of the protruding portion 4. is there. Even when the water absorbing member 5 is disposed in the protrusion 4, it is preferable to provide the through hole 43 at the protrusion end of the protrusion 4. The through-hole 43 functions as an infiltration path for the culture solution when the protruding end portion of the protrusion 4 is immersed in the culture solution in the well 401 of the multi-well plate 400.

  Next, a storage container for storing the living body hold kit 1 of the present invention will be described with reference to FIG. When the living body hold kit 1 of the present invention is used for storing and transporting specimens, it is dedicated for enclosing and isolating each protrusion 4 in order to prevent contamination of specimens held by the water absorbing member 5. It is preferable to use a storage container.

  Fig.3 (a) is sectional drawing which shows the 1st storage container 100 of 1st Embodiment. The first storage container 100 is a dedicated container for housing the living body hold kit 1 including the hollow protrusion 4 shown in FIGS. 2 (a) and 2 (c). The first storage container 100 includes a storage container main body 110 and a lid 120. The storage container main body 110 has a structure in which a flat plate portion 111 that holds the plate-like portion 3 of the living body hold kit 1 and a storage well 112 that stores the protrusions 4 of the living body hold kit 1 are integrally formed. The flat plate portion 111 is a rectangular plate-like body larger than the plate-like portion 3, and a concave portion 113 that houses the plate-like portion 3 of the biological hold kit 1 is provided on the upper surface. The accommodating well 112 has a bottomed cylindrical shape that is recessed from the flat plate portion 111, and has a position and shape that can accommodate each protrusion 4 of the living body hold kit 1. The opening of the accommodating well 112 is circular, and the outer diameter of the base end of each projection 4 of the living body hold kit 1 is very slightly larger than the inner diameter of the opening of each receiving well 112, and the living body hold kit 1 When the container is stored, the outer surface of the base end of each projection 4 of the living body hold kit 1 is fitted to the inner surface of the opening of the storage well 112.

  The lid 120 is formed integrally with the storage container main body 110 and is a plate-like body having substantially the same shape as the flat plate part 111, and the storage container main body 110 is interposed via a thin film-like hinge 114 on one side of the flat plate part 111. Is openably and closably connected. A fitting part 121 having a U-shaped cross-section that fits around the periphery of the flat plate part 111 is provided at the peripheral part of the three sides of the lid part 120 except for one side where the hinge 114 is provided, and the lid part 120 is closed and the fitting part is closed. By fitting 121 to the periphery of the flat plate part 111, the inside of the storage container body 110 of the storage container body 110 can be sealed with the lid part 120 by the hinge 114 and the fitting part 121. Moreover, when the living body hold kit 1 is housed and the lid 120 is closed on the lower surface of the lid 120, the lid 120 is fitted into the openings of the hollow protrusions 4, and the openings of the hollow protrusions 4 are opened. A short-axis frustoconical fitting projection 122 that closes the portion is provided so as to protrude at a position corresponding to each accommodating well 112. When the living body hold kit 1 is housed and the lid portion 120 is closed so as to cover the flat plate portion 111, the outer surface of the base end portion of the protrusion 4 of the living body hold kit 1 is the inner surface of the opening of the accommodating well 112 of the storage container 100. Further, the peripheral edge or the peripheral surface of the fitting projection 122 of the lid 120 presses the inner surface of the opening of the hollow projection 4 to fit. As a result, the fitting protrusion 122 of the lid 120 is in a state of sealing the protrusion 4 via the wall that forms the opening of the protrusion 4 of the living body hold kit 1 and is held by the protruding end of the protrusion 4. The water-absorbing member 5 is sealed in each accommodating well 112 and can be isolated.

  The first storage container 100 can be manufactured by integral molding, for example, by injection molding using a polyolefin resin such as polyethylene or polypropylene.

  The second storage container 100b of the second embodiment shown in the cross-sectional view of FIG. 3B is a dedicated container for housing the living body hold kit 1 having the solid protrusion 4b shown in FIG. It is. The second storage container 100b has a structure including a storage container main body 110b, a lid 120b, a clamp 130, and a packing 140. The storage container main body 110b has a structure in which a flat plate portion 111b for holding the plate-like portion 3 of the living body hold kit 1 and a receiving well 112 for storing each protrusion 4 of the living body hold kit 1 are integrally formed. The flat plate portion 111b is a rectangular plate-like body that is larger than the plate-like portion 3, and a concave portion 113 that houses the plate-like portion 3 of the biological hold kit 1 is provided on the upper surface. Further, a ring-shaped groove 115 is provided in the peripheral portion surrounding the recess 113 of the flat plate portion 111b, and a ring-shaped packing 140 is disposed in the groove 115, and the upper side of the packing 140 protrudes from the upper surface of the flat plate portion 111b. . The accommodating well 112 has a bottomed cylindrical shape that is recessed from the flat plate portion 111b, and has a position and shape that can accommodate each protrusion 4 of the living body hold kit 1. Also in the second storage container 100b, the opening of the accommodating well 112 is circular, and the outer diameter of the base end of each protrusion 4 of the living body hold kit 1 is slightly larger than the inner diameter of the opening of each well 112, and the living body When the hold kit 1 is stored, the base end of each protrusion 4 of the living body hold kit 1 is fitted into the opening of the well 112, and the opening of the well 112 is plugged with a solid protrusion 4b. And can be sealed.

  The lid 120b is a separate body from the storage container main body 110b, and is a resin plate having substantially the same shape as the flat plate portion 111b. On the inner surface of the lid portion 120b, a pressing portion 123 that presses the upper surface of the plate-like portion 3 of the living body hold kit 1 with the lid portion 120b when the lid portion 120b is closed protrudes over the region where the accommodating well 112 is present. Is provided. The clamp 130 is a ring shape made of a soft resin or rubber, and when the lid 120b is placed on the inner wall surface so as to cover the flat plate portion 111b, both ends of the lid portion 120b and the flat plate portion 111b are provided. A groove 131 having a function of pressing the periphery from above and below is formed.

  The second storage container 100b is used in such a manner that the plate-like portion 3 of the biological hold kit 1 is placed in the recess 113 of the flat plate portion 111b of the storage container main body 110b and the biological hold kit 1 is stored, and then the lid portion 120b is attached. It is placed on the flat plate portion 111b, and the clamp member 130 is fitted around the ends of the lid portion 120b and the flat plate portion 111b. By pressing the ends of the lid portion 120b and the flat plate portion 111b from above and below with the clamp member 130, the lower surface of the lid portion 120b can strongly press the packing 140 and the inside can be sealed, and the pressing portion of the lid portion 120b 123 presses the plate-like portion 3 of the living body hold kit 1 and presses the proximal end portion of the protruding portion 3b against the inner surface of the opening of the receiving well 112, thereby maintaining the sealed state of the receiving well 112 by the protruding portion 3b. In addition, the sealing of the housing well 112 can be maintained.

  Next, a method for using the living body hold kit 1 and the first storage container 100 of the present invention will be described. As a preparation stage, the living body hold kit 1 is accommodated in the first storage container 100, and the living body hold kit 1 is sealed in the first storage container 100 by covering the lid 120. As shown in FIG. 3A, the water absorbing member 5 at the protruding end of each protrusion 4 of the living body hold kit 1 indicated by a broken line is accommodated in each accommodating well 112 of the first storage container 100, The base end of the hollow projection 4 is fitted into the opening of the well 112, and the fitting projection 122 of the lid 120 is fitted into the opening of the hollow projection 4, thereby accommodating each housing. Each well 112 is sealed and isolated at its opening. The first storage container 100 containing the living body hold kit 1 is sterilized by a conventional sterilization method such as electron beam sterilization, gamma ray sterilization, high pressure steam sterilization, and stored.

  At the time of use, the lid 120 of the first storage container 100 is opened in the sterilization chamber, and the living body hold kit 1 inside is taken out. In the case of the living body hold kit 1 having the hollow protrusion 4 shown in FIGS. 2A and 2C, dispensing is performed from above the opening 41 of the plate-like part 3 with the protrusion 4 facing downward. An appropriate amount of the solution containing the sample is dropped into the hollow portion 42 of the predetermined protrusion 4 with a vessel. The dropped solution permeates through the through-hole 43 or directly into the water absorbing member 5 and is held. In this case, injection can be performed in the same multiple pattern as the multiwell plate 400. On the other hand, when the solid protrusion 4b shown in FIG. 2 (b) is provided, the living body hold kit 1 is turned over, and the specimen is placed with the water absorbing member 5 held on the protruding end of the protrusion 4b facing upward. The solution containing is dropped, and the solution is infiltrated into the water-absorbing member 5 and held. In this case, the injection is performed in a multiple pattern that is mirror-image related to the pattern of the multi-well plate 400.

  When the living body hold kit 1 holding the specimen is housed in the first storage container 100 and the lid 120 is closed, each water absorbing member 5 of the living body hold kit 1 is placed in the first storage container 100. It is enclosed in the receiving well 112 and isolated. The first storage container 100 in which the living body hold kit 1 is hermetically stored is stacked and stored in a cooler and transported to a necessary place. In this way, the biological hold kit 1 of the present invention can store and transport a large number of types of samples corresponding to the wells 401 of the multi-well plate 400 together. In addition, when the sample is a microorganism, there is a sufficient amount of air for the microorganism in the space of the sealed storage well 112 of the first storage container 100 that surrounds the periphery of one protrusion 4 during storage / transport. In addition, it can survive for a long time. In the case of a sheet-like storage / conveyance container, microorganisms could only survive in about 2 days.

  When the transported first storage container 100 is used, the surface is previously sterilized by plasma sterilization, electron beam sterilization, or the like, and then the living body hold kit 1 is taken out in the sterilization chamber. The living body hold kit 1 is placed in each well 401 of the multiwell plate 400 in which the positions of the notched portion 403 of the multiwell plate 400 and the notched portion 31 of the living body hold kit 1 are aligned and the culture solution is injected into each well 401. The plate-like portion 3 is placed on the upper surface of the multi-well plate 400 so that each of the protrusions 4 enters. Thereby, the water-absorbing member 5 held at the protruding end of the protrusion 4 is immersed in the culture solution in the well 401 of the multi-well plate 400, and the specimen can be transferred to the culture solution. When the water absorbing member 5 is held inside the protrusion 4 as shown in FIG. 2 (c), the culture solution enters the protrusion 4 through the through-hole 43, and the water absorbing member 5 is immersed therein. The specimen can then be transferred to the culture medium.

  When the air vent hole 44 as shown in FIGS. 2 (a) and 2 (c) is provided in the hollow protrusion 4, the culture medium is placed in the protrusion 4 when the protrusion 4 is immersed in the culture. The protrusion 4 can be smoothly immersed in the culture solution by entering from the air vent hole 44. Without the air vent hole 44, the protrusion 4 floats on the culture solution, and it takes time to immerse the protrusion 4 in the culture solution, resulting in poor workability.

  When the living body hold kit 1 is taken out from the multi-well plate 400, if it is pulled straight up, each projection 4 is independent, so the culture solution attached to each projection 4 moves to the adjacent well and causes turbidity. There is very little possibility.

  When the specimen is transferred to an agar medium or the like, it can be easily stamped by pressing the water absorbing member 5 at the protruding end of the protruding part 4 of the living body hold kit 1 against the agar and replicated on the medium. As a result, the multiwell plate 400 can be replicated on the agar medium in a multiple pattern.

  As described above, the living body hold kit 1 of the present invention can transfer samples of the number of types of wells to each well 401 of the multi-well plate 400 by one operation, and the water absorbing member 5 remains in the well 401. Therefore, workability is extremely good.

  After the specimen is transferred to the culture medium or agar medium, if the specimen still remains in the water-absorbing member 5, if the specimen is a microorganism, a cryoprotectant such as DMSO is dropped on the water-absorbing member 5, The kit 1 can be stored in the first storage container 100 again, sealed, and stored frozen. As described above, the living body hold kit of the present invention can be used repeatedly unlike a sheet-like storage / conveying container using a conventional adhesive sheet.

  In the above description, the first storage container 100 is used for explanation, but the same can be done using the second storage container 100b.

In the above description, the shape of the protrusion 4 is described as a truncated cone. However, the shape of the protrusion 4 can be changed as appropriate, and the method for holding the water-absorbing member 5 can also be changed as appropriate.
The storage container can also be changed as appropriate. For example, the lid can be separated from the storage container main body, and the entire periphery of the lid can be provided with a recess for fitting that fits around the periphery of the flat plate. It is. In this case, the storage container main body may be formed of relatively hard polystyrene or the like, and the lid may be formed of a soft resin such as polyethylene or polypropylene. Further, the shape of the fitting projection of the lid can be a ring shape, and any shape can be used as long as the opening of the accommodating well can be closed.

  A kit body 2 of a living body hold kit 1 for a 96-well multi-well plate as shown in FIG. 1 was integrally formed from a polypropylene sheet having a thickness of 0.4 mm by vacuum forming. The plate-like portion 3 has a size of 121 × 79 mm, the pitch of the projections 4 is 9 mm, the diameter of the openings 41 is 4 mm, the projection height of the projections 4 is about 11 mm, and the diameter of the projection end surface of the projections 4 is About 2 mm.

A through-hole 43 having a diameter of about 0.5 mm is formed in the bottom surface of the projection 4 of the kit body 2, and a rectangular air vent hole 44 having a length of 7 mm and a width of about 1 mm is formed in the projection 4. Three locations were drilled on the side of the. The lower end of the air vent hole 44 is at a position about 2 mm above the protruding end surface.
A living body holding kit 1 was prepared by attaching a water-absorbing member 5 obtained by cutting a filter paper having a thickness of 300 μm into a circular shape having a diameter of about 2.5 mm to a protruding end surface of the protruding portion 4 of the kit body 2 with a silicone adhesive.

  The lid 120 and the storage container main body 110 were integrally formed with the first storage container 100 shown in FIG. 1A by injection molding using polypropylene. The flat portion 111 of the first storage container 100 has a size of 127 mm × 85 mm, the diameter of the opening of the accommodating well 112 is about 4.7 mm, the pitch of the accommodating well 112 is 9 mm, and the accommodating well is substantially cylindrical. The shape has an outer diameter of about 5.6 mm and a height of about 15 mm. The fitting projection 122 of the lid 120 has an outer diameter of about 4 mm and a height of about 1.5 mm, and has the same taper as the projection 3.

  The produced biological hold kit 1 was stored in the first storage container 100, and the lid 120 was closed. The opening of each storage well 112 of the first storage container 100 is fitted to the base end of the projection 4 of the living body hold kit 1, and the fitting projection 122 of the lid 120 is the opening of the projection 4. By fitting, it was sealed in a stoppered state. The first storage container 100 was gamma sterilized.

The living body hold kit 1 is taken out from the first storage container 100 in the sterilization chamber, and 10 μl of a solution containing Escherichia coli is injected into the hollow portion 42 in the protruding portion 4 from the opening portion 41 of the protruding portion 4 at a fixed position by a dispenser, The specimen was absorbed by all 96 filter papers 5 through the through-hole 43. The living body hold kit 1 in which the liquid was absorbed by the filter paper 5 was housed in the first storage container 100, and the lid 120 was closed and sealed. The space around each filter paper 5 in each storage well 112 is sealed, and there is no air flow with the other filter paper 5 and the space is isolated. The biological hold kit 1 is taken out from the first storage container 100 that has been plasma sterilized in advance in a sterilization chamber after one week has passed, and the protrusion is formed on the multiwell plate 400 in which the culture solution is placed in each well 401 of 96 wells. Part 4 was infiltrated, and the filter paper 5 was immersed in the culture solution, then taken out, stored again in the first storage container 100, sealed, and stored. The multi-well plate 400 to which E. coli had been transferred could be amplified without problems.

Since the biological hold kit of the present invention can store and transport DNA, microorganisms, etc. in a multiple pattern, it can be used for storage, transport and amplification of genetic analysis, microbial assays, clinical associations, drug discovery research materials.
In addition, the storage container of the present invention can be used for the purpose of storing and transporting such a biological hold kit.

An embodiment of the living body hold kit of the present invention is shown, (a) is a top view, and (b) is a side view. It is an expanded sectional view of the projection part of the living body hold kit of the present invention, and (a)-(c) shows different embodiments. It is sectional drawing which shows the storage container of this invention, (a) and (b) show different embodiment. An example of a multiwell plate is shown, (a) is a top view, (b) is sectional drawing along the A-A 'line.

Explanation of symbols

1: Living body hold kit 2: Kit body 3: Plate-like part 4: Projection part 41: Opening part 42: Hollow part 43: Through hole 44: Air vent hole 100: First storage container 110: Storage container body 111: Flat part 112 : Receiving well 113: recessed part 114: hinge 120: lid part 121: fitting part 122: fitting projection part 123: pressing part

Claims (4)

A plate-like portion disposed on the upper surface of a multi-well plate in which a plurality of wells are arranged, and provided so as to protrude into the well at a position corresponding to each well of the plate-like portion. It has a hollow shape having a protrusion and a water absorbing member held at the protrusion end of the protrusion, and the protrusion has a hollow portion communicating with an opening provided in the plate-like portion. A biological hold kit characterized by that. The living body hold kit according to claim 1 ,
A living body hold kit, wherein a through hole is provided in a protruding end of the hollow protruding portion. In the living body hold kit according to claim 1 or 2,
The living body hold kit, wherein the water-absorbing member is disposed on an inner bottom surface of the hollow portion of the protrusion. A plate-like portion placed on the upper surface of a multi-well plate in which a plurality of wells are arranged, and provided so as to be inserted into the well at a position corresponding to each well of the plate-like portion. And a projecting portion and a water absorbing member held at the projecting end portion of the projecting portion , and the projecting portion has a hollow portion communicating with an opening provided in the plate-shaped portion. A storage container for a living body hold kit,
A storage container body having a flat plate portion on which the plate-like portion is placed, and a receiving well for receiving the protrusion portions provided at positions corresponding to the protrusion portions of the flat plate portion; A storage container, wherein each of the water-absorbing members is enclosed in each of the accommodating wells when the living body hold kit is accommodated.

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