JP4621867B2 - Plant disease control agent, method for producing the same, pesticide and fertilizer - Google Patents
Plant disease control agent, method for producing the same, pesticide and fertilizer Download PDFInfo
- Publication number
- JP4621867B2 JP4621867B2 JP2004133646A JP2004133646A JP4621867B2 JP 4621867 B2 JP4621867 B2 JP 4621867B2 JP 2004133646 A JP2004133646 A JP 2004133646A JP 2004133646 A JP2004133646 A JP 2004133646A JP 4621867 B2 JP4621867 B2 JP 4621867B2
- Authority
- JP
- Japan
- Prior art keywords
- disease control
- control agent
- extract
- plant disease
- licorice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 201000010099 disease Diseases 0.000 title claims description 55
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims description 55
- 239000003795 chemical substances by application Substances 0.000 title claims description 33
- 239000003337 fertilizer Substances 0.000 title claims description 18
- 238000004519 manufacturing process Methods 0.000 title claims description 7
- 239000000575 pesticide Substances 0.000 title description 2
- 241000196324 Embryophyta Species 0.000 claims description 44
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 claims description 28
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- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 claims description 27
- 229960004949 glycyrrhizic acid Drugs 0.000 claims description 27
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 claims description 27
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- FURUXTVZLHCCNA-AWEZNQCLSA-N liquiritigenin Chemical compound C1=CC(O)=CC=C1[C@H]1OC2=CC(O)=CC=C2C(=O)C1 FURUXTVZLHCCNA-AWEZNQCLSA-N 0.000 description 23
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Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Description
本発明は、甘草由来成分、リクイリチゲニン又はイソリクイチゲニンを有効成分とする植物病害防除剤及びその製造方法、並びに農薬及び肥料に関するものである。 The present invention relates to a plant disease control agent comprising a licorice-derived component, liquiritigenin or isoliquitigenin as an active ingredient, a method for producing the same, an agrochemical and a fertilizer.
古くから甘草(Licorice)は生薬として知られ、現在では主に食品用甘味料や医薬品・医薬部外品等の原料として使用されている。特に、その水溶性成分であるグリチルリチンには、抗炎症作用、抗潰瘍作用及び抗アレルギー作用等の優れた薬理作用があることから、広く食品、医薬品及び化粧品等に利用されている。甘草及びその成分のグラム陽性菌に対する抗菌作用については既に知られている(非特許文献1〜3参照)。また、甘草抽出物を配合した土壌改良剤(特許文献1参照)及び甘草抽出物を含有する植物生長促進剤及び該植物生長促進剤を使用した肥料(特許文献2参照)が提案されている。
しかしながら、現状の甘草抽出物では、植物病原菌に対する抗菌性は充分でないため、より効果の高いものが望まれていた。
Licorice has long been known as a herbal medicine, and is now used mainly as a raw material for food sweeteners, pharmaceuticals and quasi drugs. In particular, glycyrrhizin, which is a water-soluble component, has excellent pharmacological actions such as anti-inflammatory action, anti-ulcer action, and anti-allergic action, and thus is widely used in foods, pharmaceuticals, cosmetics, and the like. The antibacterial action of licorice and its components against gram-positive bacteria is already known (see Non-Patent Documents 1 to 3). Moreover, the soil improvement agent (refer patent document 1) which mix | blended the licorice extract, the plant growth promoter containing a licorice extract, and the fertilizer using this plant growth promoter (refer patent document 2) are proposed.
However, since the current licorice extract has insufficient antibacterial properties against phytopathogenic bacteria, a more effective one has been desired.
本発明は、上記事情に鑑みなされたものであり、広範囲にわたる植物病原菌に対する抗菌作用を有し、植物病害の防除ができる甘草由来成分、リクイリチゲニン又はイソリクイチゲニンを有効成分とする植物病害防除剤及びその製造方法、並びに植物病害防除剤を含有してなる農薬及び肥料を提供することを目的とする。 The present invention has been made in view of the above circumstances, and has an antibacterial action against a wide range of phytopathogenic fungi, and a plant disease control agent comprising a licorice-derived component, liquiritigenin, or isoliquitigenin as an active ingredient that can control plant diseases. And a method for producing the same, and an agricultural chemical and a fertilizer containing a plant disease control agent.
本発明者は、上記目的を達成するため、農業において問題になっている数種の植物病原菌に対する抗菌作用について鋭意研究を重ねてきた。その結果、甘草を中性乃至アルカリ性下で水抽出し、この抽出液を酸処理することにより得られる沈殿物をエタノール抽出し、得られたエタノール抽出液に対し活性炭脱色処理を施した後、グリチルリチンを晶析させた後の晶析母液に抗菌作用を見出し、本発明をなすに至ったものである。 In order to achieve the above-mentioned object, the present inventor has intensively studied on the antibacterial action against several phytopathogenic fungi that are problematic in agriculture. As a result, licorice was extracted with water under neutral to alkaline conditions, the precipitate obtained by acid treatment of this extract was ethanol-extracted, and the resulting ethanol extract was subjected to activated carbon decolorization, and then glycyrrhizin The antibacterial action was found in the crystallization mother liquor after the crystallization of, and the present invention was achieved.
さらに、抗菌作用を有する成分が、リクイリチゲニン又はイソリクイチゲニンであることを見出し、本発明をなすに至ったものである。 Furthermore, the present inventors have found that the component having an antibacterial action is liquiritigenin or isoliquitigenin, and have made the present invention.
従来、一般の甘草抽出物といわれているものは、熱水又は含水アルコール抽出によるものであり、グリチルリチン含量として5〜25質量%程度、フラボノイド含量として1〜5質量%程度のものが多い(非特許文献4、5参照)。また、グリチルリチンの誘導体を製造するために使用されている方法は、アルカリ抽出を行い、晶析を行った結晶物を使用し、結晶物を取り除いた母液は廃棄されるのが通常であった。これに対し、これまで廃棄されていた母液が、植物病原菌に対して抗菌作用を有することは、本発明者による新知見である。 Conventionally, what is said to be a general licorice extract is obtained by hot water or hydrous alcohol extraction, and there are many glycyrrhizin content of about 5 to 25% by mass and flavonoid content of about 1 to 5% by mass (non-existing). (See Patent Documents 4 and 5). In addition, the method used for producing a derivative of glycyrrhizin usually used an alkali extraction and crystallized crystallized material, and the mother liquor from which the crystallized material was removed was usually discarded. On the other hand, it is a new finding by the present inventors that the mother liquor that has been discarded so far has an antibacterial action against plant pathogens.
従って、本発明は
[1].甘草の中性乃至アルカリ性水抽出液を酸処理することにより生成した沈殿物のエタノール抽出液の活性炭脱色液より、グリチルリチンを晶析させた後の晶析母液からなることを特徴とする植物病害防除剤、
[2].植物病害が、イネのいもち病、苗腐病、紋枯病もしくはばか苗病、キュウリの炭疽病もしくは褐斑病、トマトの葉かび病、灰色かび病、輪紋病もしくは褐色輪紋病、ナスのすすかび病、スイカのつる枯病、ホウレンソウの萎凋病又はピーマンの斑点病であること特徴とする[1]記載の植物病害防除剤、
[3].[1]又は[2]記載の植物病害防除剤を含有してなる農薬、
[4].[1]又は[2]記載の植物病害防除剤を含有してなる肥料、
[5].甘草を中性乃至アルカリ性下で水抽出し、この抽出液を酸処理することにより得られる沈殿物をエタノール抽出し、得られたエタノール抽出液に対し活性炭脱色処理を施した後、グリチルリチンを晶析させ、その晶析母液を採取することを特徴とする植物病害防除剤の製造方法を提供する。
Therefore, the present invention
[1]. Plant disease control characterized by comprising a crystallization mother liquor after crystallization of glycyrrhizin from an activated carbon decolorization solution of an ethanol extract of a precipitate formed by acid treatment of a neutral or alkaline water extract of licorice Agent,
[2]. Plant diseases include rice blast, seed rot, coat blight or scab, cucumber anthracnose or brown spot, tomato leaf mold, gray mold, ring rot or brown ring rot, eggplant The plant disease control agent according to [1], wherein the plant disease control agent is a soy mildew disease, a watermelon vine blight, a spinach wilt disease or a bell pepper spot disease,
[3]. An agrochemical comprising the plant disease control agent according to [1] or [2],
[4]. A fertilizer comprising the plant disease control agent according to [1] or [2],
[5]. Licorice is extracted with water under neutral or alkaline conditions, and the precipitate obtained by acid treatment of this extract is extracted with ethanol. The resulting ethanol extract is subjected to activated carbon decolorization, and then glycyrrhizin is crystallized. And a method for producing a plant disease control agent characterized by collecting the crystallization mother liquor.
本発明によれば、甘草由来成分、リクイリチゲニン又はイソリクイチゲニンを有効成分とする、安全性の高い植物病害防除剤及びその製造方法、並びに農薬及び肥料を提供することができる。 ADVANTAGE OF THE INVENTION According to this invention, a highly safe plant disease control agent which uses a licorice origin component, liquiritigenin, or isoliquitigenin as an active ingredient, its manufacturing method, an agricultural chemical, and a fertilizer can be provided.
以下、本発明につき、さらに詳しく説明する。
本発明の第1の発明は、甘草の中性乃至アルカリ性水抽出液を酸処理することにより生成した沈殿物のエタノール抽出液の活性炭脱色液より、グリチルリチンを晶析させた後の晶析母液からなる植物病害防除剤である。
Hereinafter, the present invention will be described in more detail.
The first invention of the present invention is based on the crystallization mother liquor after crystallization of glycyrrhizin from the activated carbon decolorization solution of the ethanol extract of the precipitate generated by acid treatment of the neutral or alkaline water extract of licorice. It is a plant disease control agent.
本発明の植物病害防除剤の原料となる甘草は、マメ科Glycyrrihiza属に属する植物で、例えば、G.glabra、G.uralensis、G.inflata等が使用可能であるが、本発明においては、G.inflataを使用することが好ましい。また、使用部位は根、根茎、葉、茎のいずれの部位でも原料として使用することができるが、根及び/又は根茎を原料として使用することが好ましい。また、これらは、生のものを使用しても乾燥させたものを使用してもよいが、工業的に製造されているグリチルリチンの抽出原料となっている乾燥根及び乾燥根茎を原料として使用することができる。なお、甘草は生産地の名前を冠して呼ばれることが多く、例えば、東北甘草、西北甘草、新疆甘草、モンゴル産甘草、ロシア産甘草、アフガニスタン産甘草等を挙げることができる。 The licorice used as the raw material for the plant disease control agent of the present invention is a plant belonging to the genus Glycyrihiza, for example, G. glabra, G.G. uralensis, G. et al. inflata and the like can be used. It is preferable to use inflata. Moreover, although any site | part of a root, a rhizome, a leaf, and a stem can be used as a raw material, it is preferable to use a root and / or a rhizome as a raw material. These may be raw or dried, but use dry roots and dry rhizomes, which are raw materials for industrially produced glycyrrhizin, as raw materials. be able to. In addition, licorice is often called with the name of the production area, and examples thereof include Tohoku licorice, Northwest licorice, Xinjiang licorice, Mongolian licorice, Russian licorice, and Afghanistan licorice.
本発明の植物病害防除剤を得るための抽出条件としては、上記の甘草に対し中性乃至アルカリ性下で水抽出するが、通常、水抽出液のpHは7〜11、特に9〜10が好ましく、抽出温度は、冷水、温水又は熱水いずれでもよいが、5〜100℃、特に50〜100℃が好ましい。pHの調製には、アンモニア、水酸化ナトリウム、水酸化カリウム等を用いることができる。抽出時間は抽出温度によって異なるが、通常1〜72時間であり、特に2〜24時間が好ましい。 As extraction conditions for obtaining the plant disease control agent of the present invention, water extraction is carried out under neutral to alkaline conditions with respect to the above licorice. Usually, the pH of the water extract is preferably 7 to 11, particularly preferably 9 to 10. The extraction temperature may be cold water, hot water or hot water, but is preferably 5 to 100 ° C, particularly preferably 50 to 100 ° C. For adjusting the pH, ammonia, sodium hydroxide, potassium hydroxide, or the like can be used. Although extraction time changes with extraction temperature, it is 1 to 72 hours normally, and 2 to 24 hours are especially preferable.
次に、上記抽出液を酸処理することにより得られる沈殿物をエタノール抽出し、得られたエタノール抽出液に対し活性炭脱色処理を施した後、グリチルリチンを晶析させ、その結晶母液を得る。 Next, the precipitate obtained by acid-treating the above extract is subjected to ethanol extraction, and the obtained ethanol extract is subjected to activated carbon decolorization treatment, and then glycyrrhizin is crystallized to obtain a crystal mother liquor.
酸処理は、硫酸、塩酸、硝酸等の酸性溶液にて、pH2〜4の酸性で析出処理を行い、グリチルリチン等を沈殿させる。濾過により沈殿物と濾液に分ける。得られた沈殿物100質量部に対して2〜10質量部、好ましくは1〜5質量部のエタノールを加え、エタノール抽出液を得る。この抽出液を撹拌して濾過する。得られた濾液に対して2〜10質量%、好ましくは1〜5質量%の活性炭を加え、還流抽出を行う。その後、セライト、珪藻土、パーライト等の濾過助剤を用いて濾過を行い、アンモニア水、水酸化ナトリウム、水酸化カリウム等のアルカリ水を加えてpH4.0〜7.0、好ましくはpH5.0〜5.5に調整する。さらに、3〜10℃、好ましくは5〜7℃で1〜5日間、好ましくは2日間静置することでグリチルリチンを晶析させた後、濾過して、晶析したグリチルリチンを除去する。 In the acid treatment, an acid solution such as sulfuric acid, hydrochloric acid, nitric acid or the like is used to perform precipitation treatment at an acid pH of 2 to 4, thereby precipitating glycyrrhizin or the like. Separate the precipitate and filtrate by filtration. 2 to 10 parts by mass, preferably 1 to 5 parts by mass of ethanol are added to 100 parts by mass of the resulting precipitate to obtain an ethanol extract. The extract is stirred and filtered. 2 to 10% by mass, preferably 1 to 5% by mass of activated carbon is added to the obtained filtrate, and reflux extraction is performed. Thereafter, filtration is performed using a filter aid such as celite, diatomaceous earth, pearlite, etc., and alkaline water such as ammonia water, sodium hydroxide, potassium hydroxide is added to adjust the pH to 4.0 to 7.0, preferably pH 5.0 to Adjust to 5.5. Further, glycyrrhizin is crystallized by allowing to stand at 3 to 10 ° C., preferably 5 to 7 ° C. for 1 to 5 days, preferably 2 days, and then filtered to remove the crystallized glycyrrhizin.
この場合、上記エタノール抽出液を乾燥して得られた抽出物中のグリチルリチン量は、固形あたり10〜30質量%である。上記晶析工程で、このグリチルリチンの20〜50質量%、特に30〜40質量%が晶析され、除去されるように晶析を行い、晶析工程後の晶析母液を乾燥して得られた抽出物中のグリチルリチン含有量は、固形あたり5〜20質量%、特に8〜15質量%であることが好ましい。 In this case, the amount of glycyrrhizin in the extract obtained by drying the ethanol extract is 10 to 30% by mass per solid. In the above crystallization step, 20 to 50% by mass, particularly 30 to 40% by mass, of this glycyrrhizin is crystallized and removed so that the crystallization mother liquor after the crystallization step is dried. The glycyrrhizin content in the extract is preferably 5 to 20% by mass, particularly 8 to 15% by mass per solid.
本発明の植物病害防除剤は上記晶析母液からなるものであるが、晶析母液をそのまま使用することもでき、さらに、常法により濃縮して使用することもできる。また、適当な方法で抽出液を乾燥させることにより、黄褐色の甘草抽出物の粉末又は固形物として用いることもできる。 The plant disease control agent of the present invention comprises the above-mentioned crystallization mother liquor, but the crystallization mother liquor can be used as it is, and can also be used after being concentrated by a conventional method. Moreover, it can also be used as a powder or solid substance of a tan licorice extract by drying the extract by an appropriate method.
本発明の植物病害防除剤のグリチルリチン含有量は、固形あたり5〜20質量%、特に8〜15質量%が好ましく、フラボノイド類(リクイリチン、イソリクイリチン、リクイリチゲニン、イソリクイリチゲニン)の含有量は、固形あたり5〜25質量%、特に10〜20質量%が好ましい。本発明の植物病害防除剤のフラボノイド類含量は従来の甘草抽出物と比較して2〜20倍程度であり高含量である。本発明は、グリチルリチン含量としては、従来の甘草抽出物と比較して同程度であるが、高含量のフラボノイド類を含有しているため、抗菌活性が高いものである。なお、グリチルリチン含有量、フラボノイド類含有量は後述の実施例記載の測定法による。 The glycyrrhizin content of the plant disease control agent of the present invention is preferably 5 to 20% by mass, particularly preferably 8 to 15% by mass, and the content of flavonoids (liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin) is 5 to 25% by mass, particularly 10 to 20% by mass is preferred per solid. The flavonoid content of the plant disease control agent of the present invention is about 2 to 20 times that of the conventional licorice extract, which is a high content. In the present invention, the glycyrrhizin content is comparable to that of the conventional licorice extract, but has a high antibacterial activity because it contains a high content of flavonoids. The glycyrrhizin content and the flavonoid content are determined by the measurement methods described in the examples below.
本発明の植物病害防除剤は、植物病原菌に対する抗菌作用を有するものであり、特に、真核生物に属する植物病原菌(一般に糸状菌)に対する抗菌作用を有するものである。真核生物に属する病原菌には穀類、いも類、豆類、野菜、果樹、花き、特用作物、飼料作物等多数の農作物を侵し、今までに報告されている病害数(作物と病原菌の組み合わせ)は3,000以上にのぼる。主要な病原菌としてはイネではいもち病菌(ピリキュラリア オリゼ:Pyricularia oryzae)、苗腐病菌(ピシウム グラミニコラ:Pythium graminicola)、紋枯病菌(リゾクトニア ソラニ:Rhizoctonia solani)、ばか苗病菌(ジベレラ フジクロイ:Gibberella fujikuroi)、ごま葉枯病菌(コクリオボラス ミヤベナス:Cochliobolus miyabeanus)、苗立枯病菌(リゾープス オリゼ:Rhizopus oryzae)、ムギ類の赤かび病菌(フザリウム グラミネアルム:Fusarium graminicola)、ダイズの紫斑病菌(サーコスポラ キクチイ:Cercospora kikuchii)、野菜では各種野菜共通に発生する灰色かび病菌(ボトリチス シネレア:Botrytis cinerea)、うどんこ病菌(一例:スファエロテカ フリギニア:Sphaerotheca fuliginea)、菌核病菌(一例:スクレロチニア スクレロチオルム:Sclerotinia sclerotiorum)、炭疽病菌(一例:コレトトリカム オルビキュラレ:Colletotrichum orbiculare)、疫病菌(一例:ファイトフソラ メロニス:Phytophthora melonis)、各種立枯性病害を起こすフザリウム病菌(一例:フザリウム オキシスポラム:Fusarium oxysporum)、個別にはトマトの葉かび病菌(フルビア フルバ:Fulvia fulva)、輪紋病菌(アルタナリア ソラニ:Alternaria solani)、褐色輪紋病菌(コリネスポラ カシコラ:Corynespora cassiicola)、斑点病菌(ステムフィリウム リコペルシイシイ:Stemphylium lycopersici)、ナスの褐紋病菌(ホーモプシス ベサンス:Phomopsis vexans)、すすかび病菌(マイコベロシネラ ナトラシ:Mycovellosiella nattrassii)、褐色円星病菌(パラサーコスポラ エゲヌラ:Paracercospora egenula)、キュウリ(スイカ等を含む)のべと病菌(シュードペロノスポラ キュベンシス:Pseudoperonospora cubensis)、褐斑病菌(コリネスポラ カシコラ:Corynespora cassiicola)、つる枯病菌(ディディメラ ブリオネ:Didymella bryoniae)、ピーマンの斑点病菌(サーコスポラ カプシィシィ)、ホウレンソウの萎凋病菌(フザリウム オキシスポラム フォルマスペシーズ スピナシエ:Fusarium oxysporum f.sp.spinaciae)、果樹では、リンゴの斑点落葉病菌(アルタナリア マリ:Alternaria mali)、黒星病菌(ベンチュリア イネクアリス:Venturia inaequalis)、ナシの黒斑病菌(アルタナリア キクチアナ:Alternaria kikuchiana)、赤星病菌(ギムノスポランギウム アシアチウム:Gymnosporangium asiatium)、カンキツの黒点病菌(ディアポルテ シトリ:Diaporthe citri)、青カビ病菌(ペニシリウム イタリカム:Penicillium itaricum)、ブドウの晩腐病菌(グロメレラ シンギュラータ:Glomerella cingulata)、べと病菌(プラズモパラ ビチコラ:Plasmopara viticola)等が挙げられる。この中でも、イネのいもち病菌、苗腐病菌、紋枯病菌もしくはばか苗病菌、キュウリの炭疽病菌もしくは褐斑病菌、トマトの葉かび病菌、灰色かび病菌、輪紋病菌もしくは褐色輪紋病菌、ナスのすすかび病菌、スイカのつる枯病菌、ホウレンソウのの萎凋病菌又はピーマンの斑点病菌に対して、本発明の植物防除剤は特に有効である。 The plant disease control agent of the present invention has an antibacterial action against phytopathogenic fungi, and in particular has an antibacterial action against phytopathogenic fungi (generally filamentous fungi) belonging to eukaryotes. Pathogenic fungi belonging to eukaryotes invade many crops such as cereals, potatoes, beans, vegetables, fruit trees, flowers, special crops, forage crops, and the number of diseases reported so far (combinations of crops and pathogens) Is over 3,000. The main pathogens are rice blast fungus (Pyricularia oryzae), seed rot fungus (Pythium graminicura), rice blast fungus (Rhizoctonia bully burdock), , Sesame leaf blight fungus (Cochliobolus miyabeanus), seedling blight fungus (Rhizopus oryzae), wheat red mold fungus (Fusarium graminic ivy fungus) Various kinds of vegetables Gray mold fungus (Botrytis cinerea), powdery mildew fungus (example: Sphaerotheca friginea), mycorrhizal fungus (example: sclerotia sclerotole) Colletotrichum orbiculare), plague (e.g., Phytophsola melonis), Fusarium fungus causing various diseases (e.g., Fusarium oxysporum: Fusarium oxysporum), individually , Ring-rot disease fungus (Alta Ria solani: Alternaria solani), brown ring-shaped fungus (Corynespora casicola), spot fungus (Stemphyllium lycopersicii): Stemphylilium lycopersici Mycovelocinella natrashi (mycovelosiella natrassii), brown rot fungus (paracercospora genula: Paracercospora genula), cucumber (including watermelon, etc.) downy mildew (pseudoperosporus cubensis) pora cassiicola), vine blight fungus (Didimelella brioniae), spotted fungus of green pepper (Circospora capsici), spinach wilt fungus (Fusarium oxysporum formaspecies spinasum): Fusarium sp. spinciae), in fruit trees, apple leaf spot deciduous fungus (Alternaria marii), black rot fungus (Venturia inequalis), pear black spot fungus (Alternaria chrysiana fungus), : Gymnosporangium asiatium), black spot fungus of citrus (Diaporte citri), fungus of green mold (Penicillium itaricum), piromycetes of grapesviticola) and the like. Among these, rice blast fungus, seed rot fungus, coat blight fungus or leafy seedling fungus, cucumber anthracnose fungus or brown leaf fungus, tomato leaf mold fungus, gray mold fungus, ring mold fungus or brown ring fungus, eggplant The plant-controlling agent of the present invention is particularly effective against Japanese smut fungus, watermelon vine blight, spinach wilt or green pepper spot disease.
本発明の第2の発明は、リクイリチゲニン及び/又はイソリクイチゲニンを有効成分とする植物病害防除剤である。リクイリチゲニン及びイソリクイチゲニンの取得方法は、特に制限されない。リクイリチゲニン及びイソリクイチゲニンは、精製された市販のものを用いてもよいし、カンゾウ、ラッキョウ、アルファルファ等の植物から抽出してもよい。抽出方法としては、特に限定されず、水又はエタノール等の有機溶媒等で、抽出温度及び時間を適宜調整して抽出することができる。精製は、例えば、液−液分配抽出、各種クロマトグラフィー、膜分離等で行うことができる。なお、リクイリチゲニン及びイソリクイリチゲニンは、甘草の中性乃至アルカリ性水抽出液を酸処理することにより生成した沈殿物のエタノール抽出液の活性炭脱色液より、グリチルリチンを晶析させた後の晶析母液を、シリカゲルカラムクロマトグラフィーで精製して得られるものでもよい。 The second invention of the present invention is a plant disease control agent comprising liquiritigenin and / or isoliquetiginin as an active ingredient. The method for obtaining liquiritigenin and isoliquitigenin is not particularly limited. As for liquiritigenin and isoliquitigenin, a purified commercially available product may be used, or it may be extracted from plants such as licorice, rakkyo, and alfalfa. It does not specifically limit as an extraction method, It can extract by adjusting suitably extraction temperature and time with organic solvents, such as water or ethanol. Purification can be performed by, for example, liquid-liquid partition extraction, various chromatographies, membrane separation, and the like. In addition, liquiritigenin and isoliquiritigenin are crystallized after glycyrrhizin is crystallized from an activated carbon decolorization solution of an ethanol extract of a precipitate formed by acid treatment of a neutral or alkaline water extract of licorice. The mother liquor may be obtained by purification by silica gel column chromatography.
リクイリチゲニン及び/又はイソリクイチゲニンを有効成分とする植物病害防除剤において、植物病害、病原菌は上記と同様のものを挙げることができる。 In the plant disease control agent containing liquiritigenin and / or isoliquitigenin as an active ingredient, the plant diseases and pathogenic bacteria may be the same as described above.
本発明の植物病害防除剤は、そのまま又は任意の農薬成分や肥料成分と混合し農薬や肥料として用いることができる。その他の成分としては、界面活性剤、乳化剤、増量剤、保湿剤等が挙げられる。剤型は特に限定されないが、液剤、水溶剤、粉剤、粒剤、水和剤、乳剤、フロアブル剤、マイクロカプセル剤等にすることができる。使用方法としては、これらをそのまま、又は水で希釈し散布する方法が挙げられる。 The plant disease control agent of the present invention can be used as an agrochemical or a fertilizer as it is or mixed with any agrochemical component or a fertilizer component. Examples of other components include surfactants, emulsifiers, extenders, humectants and the like. The dosage form is not particularly limited, but it can be a liquid, water solvent, powder, granule, wettable powder, emulsion, flowable, microcapsule, and the like. As a method of use, a method of spraying these as they are or after diluting with water can be mentioned.
本発明の植物病害防除剤は、チッソ、リン酸、カリウムの3要素を含む複合肥料と混合して製剤化し、これを圃場に散布することにより、病害防除と施肥を同時に行うことができる。また、葉面散布肥料(液状又は粉末状の複合肥料)等を用いて混合製剤に加工し、これらを水に溶解して作物の葉面に直接散布することにより病害防除と施肥を同時に行うことができる。さらに、マンガン、ホウ素、鉄、銅、亜鉛、モリブデン、塩素、ニッケル等の必要とされる微量要素を含む微量要素肥料と混合して製剤化し、それを散布することにより、病害防除と微量要素欠乏症を同時に解決することができる。このほか、各種有機質肥料と混合して使用することもできる。 The plant disease control agent of the present invention can be formulated by mixing with a compound fertilizer containing three elements of nitrogen, phosphoric acid and potassium, and sprayed on the field, thereby enabling disease control and fertilization simultaneously. In addition, foliar spray fertilizer (liquid or powder compound fertilizer) etc. is processed into a mixed preparation, and these are dissolved in water and sprayed directly onto the leaves of the crop to simultaneously control disease and fertilize. Can do. Furthermore, by mixing and formulating with trace element fertilizers containing the required trace elements such as manganese, boron, iron, copper, zinc, molybdenum, chlorine, nickel, etc., disease control and trace element deficiency Can be solved at the same time. In addition, it can also be used by mixing with various organic fertilizers.
農薬や肥料として用いる場合、甘草の中性乃至アルカリ性水抽出液を酸処理することにより生成した沈殿物のエタノール抽出液の活性炭脱色液より、グリチルリチンを晶析させた後の晶析母液からなる植物病害防除剤の場合の配合量は、農薬全量中0.1〜90質量%が好ましく、より好ましくは1〜50質量%であり、肥料全量中0.1〜90質量%が好ましく、より好ましくは1〜50質量%である。リクイリチゲニン及び/又はイソリクイチゲニンを有効成分とする植物病害防除剤の場合の配合量は、農薬全量中0.1〜90質量%が好ましく、より好ましくは1〜50質量%であり、肥料全量中0.1〜90質量%が好ましく、より好ましくは1〜50質量%である。 When used as an agrochemical or fertilizer, a plant comprising a crystallization mother liquor after crystallization of glycyrrhizin from an activated carbon decolorization solution of an ethanol extract of a precipitate produced by acid treatment of a neutral or alkaline water extract of licorice The blending amount in the case of a disease control agent is preferably 0.1 to 90% by mass in the total amount of the agricultural chemical, more preferably 1 to 50% by mass, and preferably 0.1 to 90% by mass in the total amount of fertilizer. 1-50 mass%. The blending amount in the case of a plant disease control agent containing liquiritigenin and / or isoliquitigenin as an active ingredient is preferably 0.1 to 90% by mass, more preferably 1 to 50% by mass, and the total amount of fertilizer. 0.1-90 mass% is preferable, More preferably, it is 1-50 mass%.
本発明の植物病害防除剤は、化学合成農薬による薬剤耐性菌の出現や副作用の問題がなく、安心して使用することができる。さらに、薬剤の残留による人体や環境に対する影響がないため、野菜、樹木、花等の植物に対する病害防除に大きく貢献できる。 The plant disease control agent of the present invention is free from the appearance of drug-resistant bacteria and side effects due to chemically synthesized pesticides, and can be used with confidence. Furthermore, since there is no effect on the human body and the environment due to the residue of the drug, it can greatly contribute to disease control for plants such as vegetables, trees and flowers.
以下、実施例及び比較例を示し、本発明を具体的に説明するが、本発明は下記の実施例に制限されるものではない。なお、下記の例において特に明記のない場合は「%」は質量%である。 EXAMPLES Hereinafter, although an Example and a comparative example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to the following Example. In the following examples, “%” is mass% unless otherwise specified.
[実施例1]
甘草(Glycyrrhiza inflata)の根茎を粉砕し、チップ状にした。この甘草チップ1.0kgを10Lの3%アンモニア水(pH10)で一晩抽出した後、固液分離した。得られた抽出液に対し、1%硫酸溶液により酸性析出処理を行い、グリチルリチン等を沈殿させ、濾過により沈殿物及び抽出濾液に分けた。この内、沈殿物にエタノール1Lを加え、常温で1時間撹拌し、セライトを用いて濾過を行った。得られた濾液に活性炭60gを加え、常温で1時間、還流抽出を行った。その後、セライトを用いた濾過を行った。得られた濾液にアンモニア水を加えてpH5.0〜5.5に調整した。濾液を5℃で2日間、静置することでグリチルリチンを晶析させた後、遠心分離して結晶と濾液に分けた。得られた濾液を減圧濃縮し、炭酸ナトリウムを用いてpH7.0程度に中和し、さらに減圧濃縮、噴霧乾燥し、150gの黄褐色抽出物粉末である甘草抽出物を得た。この甘草抽出物についてHPLC分析を行った結果、グリチルリチン含量は11%であり、フラボノイド類(リクイリチン、イソリクイリチン、リクイリチゲニン、イソリクイリチゲニン)含量は15%であった。フラボノイド類含量15%のうち、リクイリチゲニン含量は5%、イソリクイチゲニン含量は3%であった。
[Example 1]
The rhizome of licorice (Glycyrrhiza inflata) was crushed into chips. 1.0 kg of this licorice chip was extracted overnight with 10 L of 3% aqueous ammonia (pH 10), and then separated into solid and liquid. The obtained extract was subjected to an acid precipitation treatment with a 1% sulfuric acid solution to precipitate glycyrrhizin and the like, and the filtrate was separated into a precipitate and an extract filtrate. Among these, 1 L of ethanol was added to the precipitate, stirred at room temperature for 1 hour, and filtered using celite. Activated carbon 60g was added to the obtained filtrate, and reflux extraction was performed at room temperature for 1 hour. Then, filtration using celite was performed. Ammonia water was added to the obtained filtrate to adjust the pH to 5.0 to 5.5. The filtrate was allowed to stand at 5 ° C. for 2 days to crystallize glycyrrhizin, and then centrifuged to separate the crystals and the filtrate. The obtained filtrate was concentrated under reduced pressure, neutralized to about 7.0 with sodium carbonate, further concentrated under reduced pressure, and spray-dried to obtain 150 g of a licorice extract as a yellow-brown extract powder. As a result of performing HPLC analysis on this licorice extract, the glycyrrhizin content was 11%, and the flavonoids (liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin) content was 15%. Of the flavonoids content of 15%, the liquiritigenin content was 5%, and the isoliquitigenin content was 3%.
[比較例1]
甘草(Glycyrrhiza inflata)の根茎を粉砕し、チップ状にした。この甘草チップ1.0kgを10Lの水で一晩抽出した後、固液分離し減圧濃縮後、噴霧乾燥させることで250gの褐色抽出物粉末である甘草抽出物を得た。この甘草抽出物についてHPLC分析を行った結果、グリチルリチン含量は10%であり、フラボノイド類(リクイリチン、イソリクイリチン、リクイリチゲニン、イソリクイリチゲニン)含量は1.5%であった。フラボノイド類含量1.5%のうち、リクイリチゲニン含量は0.1%、イソリクイチゲニン含量は0.05%であった。
[Comparative Example 1]
The rhizome of licorice (Glycyrrhiza inflata) was crushed into chips. After extracting 1.0 kg of this licorice chip overnight with 10 L of water, it was solid-liquid separated, concentrated under reduced pressure, and spray-dried to obtain 250 g of a licorice extract as a brown extract powder. As a result of performing HPLC analysis on this licorice extract, the content of glycyrrhizin was 10%, and the content of flavonoids (liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin) was 1.5%. Of the flavonoids content of 1.5%, the liquiritigenin content was 0.1%, and the isoliquitigenin content was 0.05%.
得られた甘草抽出物について、下記の植物病原菌に対する抗菌作用評価、接種試験を行った。結果を表2〜5に併記する。 About the obtained licorice extract, antibacterial action evaluation with respect to the following plant pathogenic bacteria and an inoculation test were performed. The results are also shown in Tables 2-5.
下記に、グリチルリチン含量、リクイリチゲニン含量、リクイリチン含量、イソリクイチゲニン及びイソリクイリチン含量のHPLC分析条件を示す。
(1)グリチルリチン
<HPLC条件>
カラム:Nucleosil−II 5C18 HG
溶媒 :酢酸:アセトニトリル(体積比)=60:40
流速 :1.0mL/min
検出 :254nm
温度 :40℃
注入量:20μL
(2)リクイリチゲニン、リクイリチン
<HPLC条件>
カラム:Nucleosil−II 5C18 HG
溶媒 :アセトニトリル:水:酢酸(体積比)=20:75:5
流速 :1.0mL/min
検出 :280nm
温度 :45℃
注入量:20μL
(3)イソリクイリチゲニン
<HPLC条件>
カラム:Nucleosil−II 5C18 HG
溶媒 :アセトニトリル:水:酢酸(体積比)=40:55:5
流速 :1.0mL/min
検出 :350nm
温度 :45℃
注入量:20μL
(4)イソリクイリチン
カラム:Nucleosil−II 5C18 HG
溶媒 :アセトニトリル:水:酢酸(体積比)=20:75:5
流速 :1.0mL/min
検出 :350nm
温度 :45℃
注入量:20μL
The HPLC analysis conditions for glycyrrhizin content, liquiritigenin content, liquiritin content, isoliquitigenin and isoliquiritin content are shown below.
(1) Glycyrrhizin
<HPLC conditions>
Column: Nucleosil-II 5C18 HG
Solvent: Acetic acid: Acetonitrile (volume ratio) = 60: 40
Flow rate: 1.0 mL / min
Detection: 254 nm
Temperature: 40 ° C
Injection volume: 20 μL
(2) liquiritigenin, liquiritin
<HPLC conditions>
Column: Nucleosil-II 5C18 HG
Solvent: Acetonitrile: Water: Acetic acid (volume ratio) = 20: 75: 5
Flow rate: 1.0 mL / min
Detection: 280 nm
Temperature: 45 ° C
Injection volume: 20 μL
(3) Isoliquiritigenin
<HPLC conditions>
Column: Nucleosil-II 5C18 HG
Solvent: acetonitrile: water: acetic acid (volume ratio) = 40: 55: 5
Flow rate: 1.0 mL / min
Detection: 350nm
Temperature: 45 ° C
Injection volume: 20 μL
(4) Isoliquelitin column: Nucleosil-II 5C18 HG
Solvent: Acetonitrile: Water: Acetic acid (volume ratio) = 20: 75: 5
Flow rate: 1.0 mL / min
Detection: 350nm
Temperature: 45 ° C
Injection volume: 20 μL
(1)抗菌作用評価
植物病原菌に対する培地上での菌糸伸長抑制率を測定することで評価を行った。
<検定培地の調製>
50体積%エタノールに溶解した甘草抽出物10%溶液を用いて、甘草抽出物として100、1000μg/mLの各濃度に調製したPDA培地(ニッスイ製)を調製した。シャーレに調製した培地20mLを分注し、甘草抽出物添加区の培地とした。対照区の培地は、甘草抽出物を無添加にした。
<ディスクの調製>
植物病原菌を、別に用意したPDA平板培地(培地量10mL)を用い、25℃で数日間前培養し、菌糸先端部分を直径5mmのコルクボーラーで打ち抜き、ディスクとした。
<培養>
ディスクを菌糸の面を下にして検定培地中央に置き、25℃で培養した。
<菌糸伸長量の測定>
対照区において、菌糸がシャーレの7割程度に伸長した時期に測定した。十字の線が引かれたディスク中央を基準とし、各植物病原菌の菌糸直径を測定した。1濃度につきシャーレ2〜4枚を用いて、菌糸直径を1シャーレにつき2ヶ所測定し、これらを平均して平均直径を算出した。測定結果から下記式(1)に基づいて菌糸伸長量を算出した。
菌糸伸長量=[菌糸平均直径(mm)−ディスクの直径(5mm)]/2 (1)
<菌糸伸長抑制率>
下記式(2)により、菌糸伸長抑制率を算出した。なお、菌糸伸長抑制率がマイナスである場合は、添加区の菌糸が伸長したことを示している。
<Preparation of assay medium>
Using a 10% solution of licorice extract dissolved in 50% by volume ethanol, PDA medium (manufactured by Nissui) prepared as licorice extract at concentrations of 100 and 1000 μg / mL was prepared. 20 mL of the medium prepared in the petri dish was dispensed to prepare a medium for the licorice extract added section. The licorice extract was not added to the control medium.
<Preparation of disc>
Plant pathogens were pre-cultured at 25 ° C. for several days using a separately prepared PDA plate medium (medium amount 10 mL), and the mycelial tip was punched with a cork borer having a diameter of 5 mm to form a disk.
<Culture>
The disc was placed in the center of the assay medium with the mycelium side down and cultured at 25 ° C.
<Measurement of mycelial elongation>
In the control group, it was measured at the time when the mycelium was extended to about 70% of the petri dish. Using the center of the disk with a cross line drawn as a reference, the hypha diameter of each phytopathogenic fungus was measured. Using 2 to 4 petri dishes per concentration, the mycelial diameter was measured at 2 locations per petri dish, and these were averaged to calculate the average diameter. The hyphal elongation amount was calculated from the measurement result based on the following formula (1).
Mycelial elongation amount = [average diameter of mycelia (mm) −diameter of disk (5 mm)] / 2 (1)
<Inhibition rate of hyphal elongation>
The hyphal elongation suppression rate was calculated by the following formula (2). In addition, when the hyphal elongation suppression rate is minus, it indicates that the hypha in the added section has been elongated.
植物病原菌株としては、イネのいもち病、苗腐病、紋枯病及びばか苗病、キュウリの炭疽病及び褐斑病、トマトの葉かび病、灰色かび病及び輪紋病、ナスのすすかび病、スイカのつる枯病、並びにホウレンソウの萎凋病の病原菌を用いた。 Plant pathogenic strains include rice blast, seed rot, coat blight and leafy seedling, cucumber anthracnose and brown leaf disease, tomato leaf mold, gray mold and ring mold, eggplant soot mold The pathogens of the disease, watermelon vine blight, and spinach wilt were used.
(2)接種試験
発生する病斑数を測定することで評価を行った。
<対象植物>
イネ(品種:日本晴)、キュウリ(品種:つや太郎)、トマト(品種:強力米寿2号)を用いた。
イネは1/5000アールワグネルポット、キュウリ及びトマトは素焼き鉢(5寸)に移植して1〜2ヶ月栽培し、草丈が50cm程度に伸長した時期に試験に用いた。
<植物病原菌胞子液の調製>
植物病原菌株は、イネではいもち病、キュウリでは褐斑病及び炭疽病、トマトでは褐色輪紋病、ピーマンでは斑点病の病原菌株を用いた。
イネいもち病菌はオートミール栽培、キュウリ及びトマトの病原菌はPDA培地に移植し25℃で培養した。いもち病菌についてのみ培地上の気中菌糸を絵筆で除去し、さらに培地を乾燥させる等の胞子形成促進処理を行った。1〜2週間後、培地表面に形成された病原菌胞子について絵筆を用いて蒸留水中に浮遊させ、4重ガーゼで濾過して胞子懸濁液を作製した。
この胞子懸濁液を1滴(約20μL)スライドグラスに滴下し、顕微鏡下で検鏡して概ね104個/mLの胞子濃度になるように蒸留水で調製し、植物病原菌胞子液を得た。
<試験方法>
甘草抽出物(2%)の水溶液と植物病原菌胞子液とを等量混合し、甘草抽出物として1%になるように懸濁液を調製した。この懸濁液を小型ガラス製噴霧器とコンプレッサーを用いて、植物1株当たり20〜60mL(葉から散布液が滴り落ちる程度)散布した。1処理につき、3鉢供試した。直ちに恒温接種箱に搬入し、25℃、湿度100%で2日間保湿した。その後、ガラス室に移し、約1週間後の病斑数を目視で測定した。対照区は甘草抽出物水溶液の代わりに、同量の蒸留水と植物病原菌胞子液とを等量混合した懸濁液を用いた。
(2) Inoculation test An evaluation was performed by measuring the number of lesions occurring.
<Target plant>
Rice (variety: Nipponbare), cucumber (variety: Tatsuro Taro), and tomato (variety: Strong Yoneju 2) were used.
Rice was transplanted into a 1/5000 Earl Wagner pot, cucumber and tomato in an unglazed pot (5 inches) and cultivated for 1 to 2 months, and used for the test when the plant height was extended to about 50 cm.
<Preparation of phytopathogenic spore solution>
The plant pathogenic strains used were rice blast, rice, brown spot and anthracnose for cucumber, brown ring disease for tomato, and spot disease for pepper.
Rice blast fungus was cultivated in oatmeal, and cucumber and tomato pathogens were transplanted to PDA medium and cultured at 25 ° C. Only for rice blast fungus, the aerial mycelium on the medium was removed with a paint brush, and further, spore formation promotion treatment such as drying of the medium was performed. After 1 to 2 weeks, the pathogenic spore formed on the surface of the culture medium was suspended in distilled water using a paint brush and filtered with quadruple gauze to prepare a spore suspension.
One drop (about 20 μL) of this spore suspension is dropped on a slide glass and examined under a microscope to prepare a spore solution of a phytopathogenic fungus with distilled water to a concentration of about 10 4 cells / mL. It was.
<Test method>
An equal amount of an aqueous solution of licorice extract (2%) and a phytopathogenic spore solution were mixed to prepare a suspension so as to be 1% as a licorice extract. This suspension was sprayed with a small glass sprayer and a compressor in an amount of 20 to 60 mL per plant (about the amount of sprayed liquid dripping from the leaves). Three bowls were tested per treatment. Immediately, it was carried into a constant temperature inoculation box and kept moist for 2 days at 25 ° C. and 100% humidity. Then, it moved to the glass room and measured the number of lesions about one week later visually. In the control group, instead of the licorice extract aqueous solution, a suspension obtained by mixing equal amounts of distilled water and phytopathogenic spore solution was used.
<指数の算出>
各菌株の病斑数から平均病斑数を算出した。甘草抽出物を含む懸濁液を散布した区分(甘草抽出物添加区)の平均病斑数における、植物病原菌のみ添加した区分(対照区)の平均病斑数を100としたときの相対値を、指数として下記式(3)により算出した。
The average number of lesions was calculated from the number of lesions of each strain. Relative value when the average number of lesions in the category (control group) to which only plant pathogenic bacteria were added was 100 in the average number of lesions in the category (licorice extract addition group) sprayed with the suspension containing licorice extract. The index was calculated by the following formula (3).
表1より、実施例1の甘草抽出物における菌糸伸長抑制率は、比較例1の甘草抽出物における菌糸伸長抑制率と比較して有意に高い。また、表2〜6より、実施例1の甘草抽出物における病斑数は、比較例1の甘草抽出物における病斑数と比較して有意に少ない。
これらの結果より、実施例1の甘草抽出物においては、比較例1の甘草抽出物と比較して、有意に強い抗菌作用を有しており、有意に強い植物病害防除作用が確認された。
From Table 1, the hyphal elongation inhibition rate in the licorice extract of Example 1 is significantly higher than the hyphal elongation inhibition rate in the licorice extract of Comparative Example 1. From Tables 2 to 6, the number of lesions in the licorice extract of Example 1 is significantly smaller than the number of lesions in the licorice extract of Comparative Example 1.
From these results, the licorice extract of Example 1 had a significantly stronger antibacterial action than the licorice extract of Comparative Example 1, and a significantly stronger plant disease control action was confirmed.
[試験例1]
上記の抗菌作用評価と同様の方法で、フラボノイド類であるリクイリチゲニン及びイソリクイリチゲニンそれぞれについて、トマトの褐色輪紋病の病原菌に対する培地上での菌糸伸長抑制率を測定し、抗菌作用の評価を行った。なお、リクイリチゲニン及びイソリクイリチゲニンについては、実施例1の甘草抽出物よりシリカゲルカラムクロマトグラフィーで精製したもの(純度95%)を用いた。
[Test Example 1]
In the same way as the above antibacterial action evaluation, measure the inhibition rate of mycelial elongation on the medium for the pathogens of tomato brown rot for each of the flavonoids liquiritigenin and isoliquiritigenin, and evaluate the antibacterial action Went. As for liquiritigenin and isoliquiritigenin, those purified by silica gel column chromatography from the licorice extract of Example 1 (purity 95%) were used.
表7より、リクイリチゲニン及びイソリクイリチゲニンにおける菌糸伸長抑制率は共に高く、リクイリチゲニンでは実施例1の甘草抽出物と同等であり、イソリクイリチゲニンでは実施例1の甘草抽出物と比較して1.7倍であった。比較例1の甘草抽出物と比較して実施例1の甘草抽出物では、リクイリチゲニン及びイソリクイリチゲニンが有意に高含量である。これらのことより、リクイリチゲニン及びイソリクイリチゲニンは、植物病害防除を示す有効成分であることが確認された。 As shown in Table 7, the inhibition rate of hyphal elongation in liquiritigenin and isoliquiritigenin is both high, liquiritigenin is equivalent to the licorice extract of Example 1, and isoliquiritigenin compared to the licorice extract of Example 1. 1.7 times. Compared to the licorice extract of Comparative Example 1, the licorice extract of Example 1 has a significantly higher content of liquiritigenin and isoliquiritigenin. From these, it was confirmed that liquiritigenin and isoliquiritigenin are active ingredients showing plant disease control.
[処方例1]
下記組成の農薬を常法に基づいて調製した。
実施例1の甘草抽出物 10部
キラヤ抽出物 5部
エタノール 10部
水 75部
計 100部
[Prescription Example 1]
An agricultural chemical having the following composition was prepared based on a conventional method.
10 parts of licorice extract of Example 1 5 parts of Kiraya extract 10 parts of ethanol
75 parts of water
100 copies in total
[処方例2]
下記組成の肥料を常法に基づいて調製した。
実施例1の甘草抽出物 1部
海藻粉末 5部
貝殻粉末 10部
腐葉土 84部
計 100部
[Prescription Example 2]
A fertilizer having the following composition was prepared based on a conventional method.
Licorice extract of Example 1 1 part Seaweed powder 5 parts Shell powder 10 parts
84 parts of humus
100 copies in total
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CN105384500A (en) * | 2015-11-10 | 2016-03-09 | 无锡市路华肥料科技有限公司 | Rice sheath blight preventing fertilizer |
US20210176988A1 (en) * | 2018-08-30 | 2021-06-17 | Shenyang Sinochem Agrochemicals R&D Co., Ltd. | Liquid formulation of biphenyl-containing compound and application thereof |
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US20080131536A1 (en) * | 2006-11-26 | 2008-06-05 | Ahmed Mohamed Ali Massoud | Drug for treatment of complications of diabetes mellitus |
US20100221363A1 (en) * | 2007-07-18 | 2010-09-02 | Trifolio-M Gmbh | Process for the production of a storage stable fungicidal extract of glycyrrhiza glabra for the control of phytopathogenic fungi and other plant diseases |
US7795311B2 (en) * | 2007-10-15 | 2010-09-14 | Pittsburg State University | Methods and compositions for the management of soil-borne fungal diseases |
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JPS5320428A (en) * | 1977-07-15 | 1978-02-24 | Ajinomoto Co Inc | Fungicides for agriculture and horticulture |
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CN105384500A (en) * | 2015-11-10 | 2016-03-09 | 无锡市路华肥料科技有限公司 | Rice sheath blight preventing fertilizer |
US20210176988A1 (en) * | 2018-08-30 | 2021-06-17 | Shenyang Sinochem Agrochemicals R&D Co., Ltd. | Liquid formulation of biphenyl-containing compound and application thereof |
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