JP4585329B2 - Thin slice preparation method and thin slice specimen - Google Patents

Description

  The present invention relates to a method for preparing a sliced piece specimen and a sliced specimen used in medical and bio-system analyzes such as scientific and analytical specimen analysis and microscopic observation of biological specimens, and in particular, thin slices in the form of adhesive sheets or adhesive tapes. A method for producing a thin-section specimen in which the auxiliary member is closely fixed to the surface of the solid specimen, the solid specimen is sliced to a desired thickness using a microtome, and the sliced sheet specimen is incorporated into a cassette (mount); For thin section specimens.

  For the analysis of physical and scientific samples, microscopic observation of biological samples, etc., the preparation of so-called thin slices, where the surface layer of a solid sample is sliced into a thickness of several to several tens of μm, and this is attached to a tape or a preparation, For example, as shown in FIGS. 11A to 11C, a cutter 22 and tweezers 23 of a microtome 21 (an apparatus for producing a thin slice 41 by slicing a solid sample 20 to a desired thickness by a cutter 22) are used. The workers are doing it manually. The solid sample 20 is mainly a biological sample embedded with paraffin, and is thinly sliced into a thin slice 41, which is placed on a slide glass to produce a tissue observation microscope specimen. .

In this thin section manufacturing process, handling of a thin section during and after the slicing process is an important and difficult work. This work is performed after adhering a thin slicing auxiliary member such as an adhesive tape to the surface of a solid sample. The method can be carried out relatively easily by a method of slicing a solid sample to a desired thickness and preparing a thin slice for tissue observation bonded and fixed to a slicing auxiliary member. A method for producing such a thin-section specimen is described in Patent Document 1, for example.
JP 2000-230889 A

  However, although the prior art described in Patent Document 1 can produce a large number of sliced samples by attaching thin sections on an adhesive tape, the handling is inconvenient on the adhesive tape. In addition, even if a thin slice sample is cut out on an adhesive sheet or adhesive tape and a sheet specimen or tape specimen is produced, the observation system itself such as an analysis system or a microscope or an imaging device that is currently widely used is compatible with a slide glass. Therefore, there is an inconvenience that a thin slice sample prepared with a microtome cannot be applied to these devices as they are.

  The present invention has been made to solve the above-described problems, and an object of the present invention is to provide a method for preparing a sliced piece sample and a sliced piece sample that can be applied to observation systems such as various analysis systems, microscopes, and imaging devices. To do.

The method for producing a thin-section specimen according to the present invention includes: (i) a transparent sheet-like thin-cut auxiliary member coated with an adhesive on at least one surface, and a solid sample in which a specimen to be sliced is embedded with an embedding material (Ii) a step of forming a sheet specimen including a sliced sample obtained by slicing the solid sample to a desired thickness; and (iii) a slide glass for microscopic observation. And a step of sandwiching between an upper cassette plate and a lower cassette plate formed in a rectangular shape having the same size to form a thin slice specimen.

The method for preparing a sliced specimen according to the present invention includes (a) embedding a transparent tape-like thin-cut auxiliary member coated with an adhesive on at least one surface with a sample to be sliced with an embedding material. A step of closely fixing to the surface of the solid sample, (b) a step of forming a tape sample including a sliced sample obtained by slicing the solid sample to a desired thickness, and (c) repeating the steps (a) and (b). A tape specimen in which the thin slice sample is continuously tightly fixed on the tape-shaped thin slice auxiliary member, and (d) cutting the tape specimen into a desired length to obtain a sheet specimen. And (e) a step of sandwiching the sheet specimen between an upper cassette plate and a lower cassette board formed in a rectangular shape substantially the same size as a microscope slide glass to obtain a thin slice specimen It is characterized by comprising.

The thin slice specimen according to the present invention includes a sheet specimen having a transparent sheet-like thin-cut auxiliary member coated with an adhesive on at least one surface, and a thin-cut sample closely fixed on the thin-cut auxiliary member; In order to sandwich and hold the sheet specimen so that the cut sample is in an observable state, it is formed in a rectangular shape substantially the same size as a slide glass for microscope observation, and an upper cassette plate for sandwiching the sheet specimen and And a lower cassette plate .

The outer dimensions of the upper and lower cassette plates are formed in a rectangular shape having a size substantially equivalent to that of a slide glass for microscopic observation. Incidentally, the standard size of the slide glass is defined by standards such as JIS R3703-1998 or ISO 8037, and specifically, the thickness is 0.8 to 1.5 (preferably 1.2 mm) and the width is 26 mm. The standard length is about 76 mm.

  The cassette has a lower cassette plate on which a sheet specimen is placed and a window (hole) through which a sliced sample can be observed, and is connected to the lower cassette plate by a hinge so that the sheet specimen is sandwiched between the lower cassette plate and the lower cassette plate. And a cassette plate. A sheet specimen can be attached to at least one of these upper and lower cassette plates. In addition, the thin slice sample on the sheet specimen can be sandwiched between cover sheets made of substantially the same material as the thin-cut auxiliary member.

  Furthermore, a sheet sample can also be produced by cutting a tape sample in which a thin slice sample is continuously tightly fixed on a tape-shaped thin-cutting auxiliary member into a predetermined length.

  Further, the upper cassette plate may have a locking portion at the center of the long side, and this locking portion may be locked at the center of the long side of the lower cassette plate.

  Further, a protrusion may be formed on either the upper cassette plate or the lower cassette plate, a groove may be formed on either the upper cassette plate or the lower cassette plate, and the protrusion may be fitted into the groove. Alternatively, a concave taper is formed on either the upper cassette plate or the lower cassette plate, a convex taper is formed on the other of the upper cassette plate or the lower cassette plate, and the concave taper is fitted to the convex taper. You may make it do. In this way, the sheet specimen sandwiched between the upper and lower cassette plates is firmly held at a predetermined position, and the sheet specimen is not displaced.

  According to the present invention, a thin slice sample prepared by a microtome can be used as it is for an observation system such as various currently used analysis systems and microscopes or imaging devices. In particular, by making the cassette of the thin section specimen of the present invention the same size as a conventional slide glass, it is compatible with various inspection instruments, microscopes, storage tools, and storages, so its use is wide. It will be a thing.

  Various best modes for carrying out the present invention will be described below with reference to the accompanying drawings.

(First embodiment)
A first embodiment of the present invention will be described with reference to FIG.
A thin-section specimen 1A of the first embodiment is obtained by incorporating a sheet specimen 4 into a cassette 5A. The cassette 5A includes a lower cassette plate 51 on which the sheet specimen 4 is placed, and a pair of left and right upper cassette plates 52 that press the sheet specimen 4 from above, and is defined in JIS R 3703-1998 or ISO 8037 as a whole. It is made to have a shape and size corresponding to a standard size glass slide. Each of the upper cassette plates 52 is connected to the lower cassette plate 51 by a hinge 53.

  The upper cassette plate 52 has an L-shaped locking portion 54 formed at the front end thereof, and the locking portion 54 is hooked on a notch 55 of the lower cassette plate 51. The notch portion 55 constitutes a lock mechanism. That is, when the locking portion 54 is locked to the notch portion 55, the locking portion 54 is flush with the long side of the lower cassette plate 51. The upper and lower cassette plates 51 and 52 are not limited to being made of a transparent material, but may be made of an opaque material such as various resins.

  The sheet specimen 4 is composed of a thin slice sample 2 and a thin slice auxiliary member 3. An adhesive is applied or pasted on at least one surface of the thin-cut auxiliary member 3, and the thin-cut sample 2 is bonded to the adhesive surface of the thin-cut auxiliary member 3. As shown in FIG. 2 (a), the sheet specimen 4 can be placed in a state where a hole 5w is formed in the center of the cassette plate 60 so that the sliced sample 2 can be seen from above, and (b) of FIG. ), The thin slicing auxiliary member 3 can be bonded to the cassette plate 60. Further, as shown in FIG. 3A, an adhesive portion in which the backing paper 9 is attached to a part of the cassette plate 61 may be formed, and the backing paper 9 may be peeled off to attach the sheet specimen 4. Further, as shown in FIGS. 1B and 2C, a transparent cover plate 7 made of a glass plate or a plastic plate may be fitted and adhered with an adhesive.

  When preparing a thin slice specimen, as shown in FIG. 1A, the sheet specimen 4 is placed on the lower cassette plate 51, the pair of upper cassette plates 52 are closed, and the locking portions 54 are notched 55. As shown in FIG. 1 (b), a transparent cover plate 6 is fitted and bonded with an adhesive so that the sliced sample 2 can be observed from above with a microscope or the like. Also good. Further, as shown in FIG. 3B, the sheet specimen 4 is placed on the lower cassette plate 51, the sheet specimen 4 is covered with the transparent cover sheet 6, and the pair of upper cassette plates 52 are closed. As shown in (b), both end portions of the cover sheet 6 may be pressed. Further, the sheet specimen 4 may be individually produced using a microtome 21 as shown in FIG. 8, or may be produced from a tape specimen 30 as shown in FIGS.

  According to the present embodiment, by incorporating a sheet specimen produced by a microtome into a cassette having a simple structure, it becomes possible to handle it in the same manner as a conventional slide glass, and it is possible to observe various analysis systems, microscopes, imaging devices, and the like. The thin sliced sample can be easily observed in the system. In addition, because the notch is provided so that the locking part is flush with the long side of the lower cassette plate, the overall appearance of the thin slice specimen is very close to that of the slide glass, There is an advantage that compatibility between the two is improved.

(Second Embodiment)
Next, a second embodiment will be described with reference to FIG.
In this embodiment, a thin slice specimen is produced by sticking the sheet specimen 4 on one cassette plate 60. A rectangular hole 5w that functions as an observation window is formed in the center of one cassette plate 60, and as shown in FIG. 2 (a), the sliced sample 2 can be seen from above through the hole 5w. The sheet specimen 4 is pasted with the adhesive sheet 3. That is, the sheet specimen 4 is attached to the lower surface of the cassette plate 60. This state is shown in FIG. Further, as shown in FIG. 2 (c), a transparent cover plate 60 is further fitted into the hole 5w of the thin-section specimen of FIG. 2 (b) to protect the sheet specimen 4. The cover plate 60 may be a transparent material, and glass or plastic is suitable.

  According to the present embodiment, since only one cassette plate is required, the thin-section specimen has a simpler structure and is easy to handle.

(Third embodiment)
Next, a third embodiment will be described with reference to FIG.
Also in the present embodiment, the sheet specimen 4 is pasted on one cassette plate 61 to produce a thin slice specimen. A rectangular hole 5w that functions as an observation window is formed in the center of one cassette plate 61, and a pair of left and right adhesive portions is formed by applying an adhesive on both sides of the hole 5w. A backing paper 9 is pasted on the adhesive part.

  As shown in FIG. 3A, the sheet specimen 4 is affixed to the central portion of the cassette plate 61, the backing paper 9 is peeled off, and a transparent cover sheet 6 is affixed to the pair of left and right adhesive parts. Protect. This state is shown in FIG. The cover sheet 6 may be a transparent material, and a resin film is suitable.

  According to the present embodiment, since only one cassette plate is required, the thin-section specimen has a simpler structure and is easy to handle. Moreover, since it is only necessary to affix the cover sheet instead of the cover plate of the second embodiment, there is an advantage that the preparation work is simplified and the specimen can be manufactured in a short time.

(Fourth embodiment)
Next, a fourth embodiment will be described with reference to FIG.
A thin-section specimen 1B of the fourth embodiment is obtained by incorporating a sheet specimen 4 into a cassette 5B. The cassette 5B includes a lower cassette plate 51B on which the sheet specimen 4 is placed, and an upper cassette plate 52B that presses the sheet specimen 4 from above, and has a standard size defined in JIS R 3703-1998 or ISO 8037 as a whole. It is manufactured to have a shape and size corresponding to a slide glass. The upper cassette plate 52B has a rectangular hole 5w that functions as an observation window in the center thereof, and is connected to the lower cassette plate 51B by a hinge 53. A rectangular hole 5w is also formed at the center of the lower cassette plate 51B at a position corresponding to the hole 5w of the upper cassette plate 52B. A locking portion 54B having a wedge-shaped cross section is formed at the center of the long side of the upper cassette plate 52B, and the locking portion 54B is hooked to the center of the long side of the lower cassette plate 51B.

  The label 8 is placed on the lower cassette plate 51B, and is sandwiched between the transparent upper cassette plates 52B so that the label 8 can be seen. On the label 8, various information such as a description of the sample 2, various identifications and numbers, or a bar code can be written.

(Fifth embodiment)
Next, a fifth embodiment will be described with reference to FIG.
A thin-section specimen 1C of the fifth embodiment is obtained by incorporating a sheet specimen 4 into a cassette 5C. The cassette 5C includes a lower cassette plate 51C on which the sheet specimen 4 is placed, and an upper cassette plate 52C that presses the sheet specimen 4 from above, and has a standard size defined in JIS R3703-1998 or ISO 8037 as a whole. It is manufactured to have a shape and size corresponding to a slide glass. The upper cassette plate 52C has a rectangular hole 5w at the center thereof, and is connected to the lower cassette plate 51C by a hinge 53. A rectangular hole 5w is also formed at the center of the lower cassette plate 51C at a position corresponding to the hole 5w of the upper cassette plate 52C. A locking portion 54C having a rectangular cross section is formed at the center of the long side of the upper cassette plate 52C, and the locking portion 54C is fitted into the cutout portion 55C at the center of the long side of the lower cassette plate 51C. Yes.

  According to the present embodiment, the cutout portion is provided so that the locking portion is flush with the long side of the lower cassette plate, so that the overall appearance of the thin slice specimen is very close to that of the slide glass. Compatibility with conventional equipment is improved.

(Sixth embodiment)
Next, a sixth embodiment will be described with reference to FIG.
A sliced specimen 1D of the sixth embodiment is obtained by incorporating a sheet specimen 4 into a cassette 5D. The cassette 5D includes a lower cassette plate 51D on which the sheet specimen 4 is placed, and an upper cassette plate 52D that presses the sheet specimen 4 from above. The cassette 5D has a standard size defined in JIS R3703-1998 or ISO 8037 as a whole. It is manufactured to have a shape and size corresponding to a slide glass. The upper cassette plate 52D has a rectangular hole 5w in the center, and is connected to the lower cassette plate 51D by a hinge 53. A rectangular hole 5w is also formed at the center of the lower cassette plate 51D at a position corresponding to the hole 5w of the upper cassette plate 52D. A locking portion 54D having a wedge-shaped cross section is formed at the center of the long side of the upper cassette plate 52D, and the locking portion 54D is hooked on the center of the long side of the lower cassette plate 51D.

  Linear protrusions 56 are formed on both sides of the hole 5w of the upper cassette plate 52D from the end to the end in the cassette width direction, and linear grooves 57 are formed on both sides of the hole 5w of the lower cassette plate 51D. It is formed from the cassette width direction end to the end. When the upper cassette plate 52D is superposed on the lower cassette plate 51D, the protrusions 56 are fitted into the groove portions 57, respectively.

  According to the present embodiment, in addition to the locking portion, the upper and lower cassette plates are further provided with projections and grooves, respectively, so that the sheet sample sandwiched between the upper and lower cassette plates is securely held in place, and the sheet sample Will not shift.

(Seventh embodiment)
Next, a seventh embodiment will be described with reference to FIG.
A thin slice specimen 1E of the seventh embodiment is obtained by incorporating a sheet specimen 4 into a cassette 5E. The cassette 5E includes a lower cassette plate 51E on which the sheet specimen 4 is placed, and an upper cassette board 52E that presses the sheet specimen 4 from above, and as a whole is of a standard size defined in JIS R3703-1998 or ISO 8037. It is manufactured to have a shape and size corresponding to a slide glass. The upper cassette plate 52E has a rectangular hole 5w at the center thereof, and is connected to the lower cassette plate 51E by a hinge 53. A rectangular hole 5w is formed at the center of the lower cassette plate 51E at a position corresponding to the hole 5w of the upper cassette plate 52E. A locking portion 54E having a wedge-shaped cross section is formed at the center of the long side of the upper cassette plate 52E, and the locking portion 54E is hooked on the center of the long side of the lower cassette plate 51E.

  Concave taper portions 58 are formed on both sides of the hole 5w of the upper cassette plate 52E from end to end in the cassette width direction, and convex taper portions 59 are formed on both sides of the hole 5w of the lower cassette plate 51E in the cassette width direction. It is formed from end to end. When the upper cassette plate 52E is superposed on the lower cassette plate 51E, the concave taper portions 58 are fitted and adhered along the convex taper portions 59. The inclination of the concave taper portion 58 and the convex taper portion 59 is desirably a gentle angle (for example, 15 ° to 30 °) with respect to the cassette surface.

  According to this embodiment, in addition to the locking portion, the upper and lower cassette plates are provided with a concave taper portion and a convex taper portion, respectively, so that the sheet specimen sandwiched between the upper and lower cassette plates is firmly held at a predetermined position. The sheet specimen will not be displaced.

  Next, an outline of a procedure for producing a sheet specimen will be described with reference to FIGS.

  First, the transparent adhesive sheet 3 which apply | coated the adhesive to at least one side is affixed on the surface of the solid sample 20 on the microtome 21, as shown to Fig.8 (a). The pressure-sensitive adhesive sheet 3 corresponds to a thin cutting auxiliary member. Next, the cutter 22 is manually moved in the direction of the arrow in FIG. 8B to slice the solid sample 20, and the sheet specimen 4 including the sliced sample 2 is peeled from the cutter 22 as shown in FIG. 8C. take. The sheet specimen 4 produced in this way was incorporated into the cassettes 5A to 5E described above, and thin slice specimens 1A to 1E were produced.

Next, an outline of a method for producing a thin-section sample using an adhesive tape and an observation method thereof will be described with reference to FIGS.
The solid sample 20 is adsorbed to the transport table 40 at predetermined intervals by an electrostatic adsorption method. On the other hand, after the adhesive tape 30 is continuously fed from a feeding reel (not shown) to the solid sample 20 on the transport table 40 and the adhesive tape 30 is pressed and bonded to the solid sample 20 by a pressing roller (not shown), the cutter 22 is used. The solid sample 20 is sliced. By repeating these steps, it is possible to produce a tape specimen 32 to which the sliced sample 2 is adhered at predetermined intervals.

  Next, an outline of a procedure for producing a tape specimen will be described.

  The adhesive tape 30 is fed in the direction of the arrow as shown in FIG. 9A, and the feeding of the adhesive tape 30 is stopped at the position shown in FIG. 9B. Next, the pressure-sensitive adhesive tape 30 is pushed up by a pressure roller (not shown), and the surface of the solid sample 20 is brought into close contact with the pressure-sensitive adhesive tape 30 as shown in FIG. Further, the cutter 22 is advanced in the direction of the arrow shown in FIG. At this time, air (cold air) 46 is blown into the gap between the cutter 22 and the adhesive tape 30 to enhance the detachability of the cutter 22 from the adhesive tape 30. As a result, the adhesive tape 30 is not attached to the cutter 22 and entangled with the cutter 22 as shown in FIG. Next, as shown in FIG. 9F, a not-shown pressing roller is retracted upward, and the cutter 22 is further retracted to the original home position as shown in FIG. 9G. By repeating such an operation, a tape specimen 32 in which a plurality of sliced samples 2 are adhered onto the adhesive tape 30 is produced.

  The obtained tape specimen 32 is cut along a cutting line 48 as shown in FIG. As a result, a sheet sample 4 having a predetermined size is obtained from the tape sample 32. The obtained sheet specimen 4 is incorporated in each of the cassettes 5A to 5E. Thereby, the thin slices 1A to 1E are produced.

  INDUSTRIAL APPLICABILITY The present invention can be used for the preparation of thin section specimens used in medical and bio-system analyzes such as scientific and scientific sample analysis and microscopic observation of biological samples.

(A) is a perspective view which shows the state which opened the cassette of the thin section specimen which concerns on embodiment of this invention, (b) is a perspective view which shows the state which closed the cassette of the thin section specimen of (a). (A) is an exploded perspective view showing a thin-section sample prepared by incorporating a thin-section sample into one cassette plate as another embodiment, and (b) is a state in which a sheet sample is incorporated on the cassette plate of (a). The side sectional view which shows the thin section sample of (b), (c) is a side sectional view which shows the thin section sample which further fitted the cover plate in the thin section specimen of (b). (A) is an exploded perspective view showing a thin-section specimen prepared by incorporating a thin-section specimen into one cassette plate as another embodiment, and (b) is a cover sheet in which a sheet specimen is placed on the cassette board of (a). The sectional side view which shows the thin section sample of the state covered with. The perspective view which shows the thin section sample of other embodiment. The perspective view which shows the thin section sample of other embodiment. (A) is a perspective view which shows the thin-section sample of other embodiment, (b) is a sectional side view of the thin-section sample of (a). (A) is a perspective view which shows the thin-section sample of other embodiment, (b) is a sectional side view of the thin-section sample of (a). (A)-(c) is a perspective view which shows the preparation procedures of the thin section sample of this invention. (A)-(g) is process drawing which shows the thin slice sample preparation method using the adhesive tape. The top view when cut | disconnecting the adhesive tape after sample collection, and producing a sheet | seat sample. (A)-(c) is a perspective view which shows the preparation procedure of the conventional sheet | seat sample.

Explanation of symbols

1A, 1B, 1C, 1D, 1E ... thin section specimen,
2 ... sliced sample,
3 ... Adhesive sheet (thin cutting auxiliary member),
4 ... sheet specimen,
5A, 5B, 5C, 5D, 5E, 60, 61 ... cassette, 5w ... hole (window),
51, 51B, 51C, 51D, 51E ... lower cassette plate,
52, 52B, 52C, 52D, 52E ... upper cassette plate,
53 ... Hinge,
54, 54B, 54C, 54D, 54E ... locking portion (lock mechanism),
55, 55C ... Notch fitting part (lock mechanism),
56 ... projection, 57 ... groove,
58 ... concave taper portion, 59 ... convex taper portion,
6 ... cover sheet, 7 ... cover plate, 8 ... label, 9 ... backing paper for adhesive part,
60, 61 ... cassette plate,
20: Solid sample,
21 ... Microtome,
22 ... Cutter,
23 ... tweezers,
30 ... Adhesive tape (thin cutting auxiliary member), 32 ... Tape specimen,
40 ... transport table,
48 ... cutting line.

Claims (14)

(I) a step of fixing and fixing a transparent sheet-like thin-cut auxiliary member coated with an adhesive on at least one surface to the surface of a solid sample in which a specimen to be sliced is embedded with an embedding material;
(Ii) a step of forming a sheet sample including a sliced sample obtained by slicing the solid sample into a desired thickness;
(Iii) sandwiching the sheet specimen between an upper cassette plate and a lower cassette board formed in a rectangular shape substantially the same size as a slide glass for microscopic observation to form a thin slice specimen;
A method for producing a thin-section specimen characterized by comprising: (A) a step of fixing and fixing a transparent tape-shaped thin-cut auxiliary member coated with an adhesive on at least one surface to a solid sample surface in which a specimen to be sliced is embedded with an embedding material;
(B) a step of forming a tape specimen containing a sliced sample obtained by slicing the solid sample into a desired thickness;
(C) By repeating the steps (a) and (b), a step of forming a tape specimen in which the sliced sample is continuously tightly fixed on the tape-shaped sliced auxiliary member;
(D) cutting the tape specimen into a desired length to obtain a sheet specimen;
(E) a step of sandwiching the sheet specimen between an upper cassette plate and a lower cassette board formed in a rectangular shape substantially the same size as a slide glass for microscopic observation to form a thin slice specimen;
A method for producing a thin-section specimen characterized by comprising: The method according to claim 1 , wherein the upper cassette plate has a hole in at least a portion where the sliced sample is closely fixed. Wherein the cassette plate has a hole in at least portions of sliced sample was closely fixed, either the lower cassette plate according to claim 1 or 2, characterized in that it is hinged on said cassette plate 1 The method described in the paragraph . 5. The method according to claim 3 , wherein at least the lower cassette plate is made of a material substantially equivalent to the thin-cut auxiliary member. The method according to claim 3 , wherein the hole of the upper cassette plate in a state where the sheet specimen is adhered is sealed with a transparent material member and an encapsulating material. 6. The hole of the upper cassette plate in a state where the sheet specimen is adhered is sealed with a member and a sealing material that are substantially the same material as the thin-cut auxiliary member . The method according to claim 1 . A transparent sheet-like thin-cut auxiliary member coated with an adhesive on at least one surface, and a sheet specimen having a thin-cut sample that is tightly fixed on the thin-cut auxiliary member;
In order to sandwich and hold the sheet specimen so that the sliced sample is in an observable state, the upper cassette is formed in a rectangular shape substantially the same size as a microscope observation slide glass and sandwiches the sheet specimen. A thin-section specimen comprising a plate and a lower cassette plate . 9. The thin slice specimen according to claim 8 , wherein the sheet specimen is obtained by cutting a tape specimen in which a thin slice sample is continuously tightly fixed on a tape-shaped thin cutting auxiliary member into a predetermined length. The thin slice specimen according to any one of claims 8 and 9, wherein the upper cassette plate has a hole in at least a portion where the thin sliced sample is closely fixed. The lower cassette plate and the upper cassette plate are connected to each other by a hinge, according to any one of claims 8 to 10, wherein said that the sheet sample is attached to at least one of the upper and lower cassette plate Thin section specimen.
Furthermore, the said upper cassette board has a latching | locking part in the long side center, This latching | locking part is latched by the long side center of the said lower cassette board, The any one of Claim 10 or 11 characterized by the above-mentioned. Thin section specimen as described in item . Furthermore, it has a projection part formed in any one of the said upper and lower cassette board, and a groove part formed in the other of the said upper and lower cassette board so that a fitting to this projection part is carried out, The thru | or 10 characterized by the above-mentioned. 13. The thin-section sample according to any one of 12 above. Furthermore, it has a concave taper part formed in any one of the said upper and lower cassette board, and a convex taper part formed in the other of the said upper and lower cassette board, The any one of Claim 10 thru | or 12 characterized by the above-mentioned. Thin section specimen as described in item .

Images