JP4453636B2 - Safety cabinet for cell culture adjustment - Google Patents

Description

The present invention relates to a safety cabinet for cell culture adjustment, and more particularly to a safety cabinet for cell culture adjustment suitable for subculture of cells collected from a living body .

In recent therapeutic medicine, research on regenerative medicine in which cells collected from a living body are cultured to regenerate a lost living tissue has been actively conducted. Patent Document 1 discloses a cell culture system for regenerative medicine. The cultured cells are used only for the provider when the cell provider is given regenerative medicine. Therefore, in this type of cell culture, it is necessary to strictly manage the association between the cultured cells and the cell donor. For this reason, every time the cultured cells are transferred to the container, Patent Document 1 describes a method of inputting and managing unique identification information attached to the container before and after the transfer. According to the method described in Patent Document 1, the association between the cultured cell and the cell provider is successively passed on. For this reason, it is possible to prevent a mistake between the cultured cells and the cell provider.
Japanese Patent Laid-Open No. 2004-119

By the way, in this type of cell culture operation, almost all processing operations are carried out while stepping through each stage in a safety cabinet for cell culture adjustment. For this reason, when the cell culture operation is composed of, for example, several tens of steps, the necessary raw materials, equipment, and chemicals are brought into the safety cabinet for each step, and the stored materials and waste are removed after the processing is completed. Sorting and storing items temporarily in a predetermined area. Then, in the next process, necessary raw materials, materials, and chemicals are again brought into the safety cabinet and processed. For this reason, in cell culture operations composed of a plurality of processes, the types and quantities of materials such as raw materials, materials, and medicines repeatedly brought into the safety cabinet reach a huge number. It is very difficult to manage these raw materials, materials and chemicals without fail during the cell culture process. Although the method described in Patent Document 1 can cope with the minimum need to prevent accidents caused by mixing between cultured cells and cell providers, it manages a large number of various devices appropriately and without mistakes. Has its limits.
An object of the present invention is to provide a safety cabinet for cell culture adjustment that can improve the problems of the prior art and can manage an enormous number of various equipments handled in cell culture operations without mistakes. is there.

In order to achieve the above object, a safety cabinet for cell culture adjustment according to the present invention is inserted into an insertion port for equipment used for cell culture adjustment, and a storage portion for storing equipment inserted from the insertion port. A reader for recognizing the identification information carried by the device at the insertion port, a collation unit for collating whether the identification information recognized by the reader matches the identification information registered in advance, and the collation unit A safety cabinet for cell culture adjustment provided with an output means for outputting a collation result, wherein the reader antenna having the entire width of the insertion port as a detection range is installed at the front surface of the insertion port. Features .

The safety cabinet for cell culture adjustment configured as described above is provided with a shutter that opens and closes the insertion port in the vertical direction, and warns when the opening height of the shutter exceeds a height set within the effective detection range of the antenna. It is desirable to provide warning means for emitting.

In addition, the safety cabinet for cell culture adjustment according to the present invention carries an insertion port for equipment used for cell culture adjustment, a housing portion for housing equipment inserted from the insertion port, and an equipment to be inserted. A reader for recognizing the identification information at the insertion port, collation means for collating whether or not the identification information recognized by the reader matches previously registered identification information, and an output for outputting a collation result by the collation means A safety cabinet for adjusting cell culture, comprising: a shutter for opening and closing the insertion port in the vertical direction; and an antenna installed at a lower end of the shutter.
Also, a storage unit for storing equipment used for cell culture adjustment, a reader for recognizing identification information carried by each of the plurality of equipments stored in the storage unit, and each identification information recognized by the reader Is a safety cabinet for cell culture adjustment, comprising: a collating unit that collates whether or not the identification information matches with previously registered identification information; and an output unit that outputs a collation result by the collating unit. The reader antenna having the entire width of the front part of the floor as a detection range is installed.

Further, the safety cabinet for cell culture adjustment includes a storage unit for storing equipment used for cell culture adjustment, a reader for recognizing identification information carried by each of the plurality of equipment stored in the storage unit, A safety cabinet for cell culture adjustment, comprising: verification means for verifying whether each identification information recognized by the reader matches previously registered identification information; and output means for outputting a verification result by the verification means The reader antenna is installed on the entire floor surface of the storage area. Further, the reader antenna may be installed on the ceiling surface of the housing portion.

According to the present invention , the identification information is recognized by the reader when all of the equipment inserted into the accommodating portion passes through the insertion port or is accommodated in the accommodating portion, and the identification information is registered in advance. Whether or not it matches the information is collated. For this reason, accidents related to human life such as mistakes between cell providers and cultured cells can be reliably prevented, and materials such as culture dishes, centrifuge tubes, pipettes and packs used for cell culture, and chemicals such as culture fluids and washing fluids can be prevented. The type and quantity can be managed precisely.

  In addition, since the warning is issued when the height of the insertion slot formed by opening and closing the shutter exceeds the set height, it becomes easy to manage the height of the insertion slot within the set height, The identification information of each device passing through can be accurately read by the reader. Therefore, according to the present invention, an enormous number of various devices handled in the cell culture operation can be managed accurately and without error.

  FIG. 1 is a perspective view showing a first embodiment of a safety cabinet for cell culture adjustment according to the present invention. The safety cabinet 10 has a shutter 12 that opens and closes in a vertical direction on the front surface in a cubic shape, and the inside serves as a housing 14 for various equipment used for cell culture. By pulling up the shutter 12, an insertion port 16 for equipment having a height H is formed.

  FIG. 1 shows a situation in which various equipment necessary for cell culture is housed in the housing section 14 of the safety cabinet 10. The housing section 14 already has a plurality of culture dishes 20, centrifuge tubes 22, and the like as equipment. Contained. The shutter 12 is formed of a transparent material, and the operator 18 of the safety cabinet 10 performs necessary adjustment work while visually checking the inside of the housing portion 14. When the equipment necessary for cell culture is accommodated in the accommodating portion 14, the operator 18 inserts the culture dish 20 such as the equipment into the insertion port 16 one by one. An IC tag 24 is affixed to each device, and an ID number for identifying each device is stored in an IC chip constituting the IC tag 24. A reader 25 capable of reading the ID number stored in the IC tag 24 is installed on the front surface of the safety cabinet 10, and an antenna 26 for the reader 25 is arranged over the entire width of the insertion slot 16. Therefore, the ID number stored in the IC tag 24 is always detected by the antenna 26 when each device (for example, the culture dish 20) inserted by the operator 18 passes through the insertion slot 16, and is read by the reader 25. The ID number recognized by the reader 25 is transmitted to a verification unit (not shown). In this verification means, an ID number relating to the equipment to be inserted into the safety cabinet 10 in the current time zone of the day is registered in advance based on the work instruction created by the overall management system of the culture facility. The collation means collates whether or not the ID number relating to each device recognized by the reader 25 matches the ID number registered in advance. Then, the verification result is output to the monitor 28 attached to the side of the safety cabinet 10.

  FIG. 2 is an explanatory diagram showing the relationship between the safety cabinet and the overall management system. The culture facility mainly accepts cells collected from living organisms and temporarily stores them, cell reception areas for storing received cells and cultured cells in centrifuge tubes, storage or centrifugation, and cells divided into culture dishes. A culture area for culturing in the incubator, a material area for storing and supplying new culture dishes, centrifuge tubes, pipettes, packs, etc., which are necessary for cell culture, and chemicals such as culture solution and washing solution used for cell culture Chemical area for storage and supply, cell storage area for storing cultured cells in a pack, waste area for temporarily storing used materials and chemicals as waste, and cell culture adjustment Configure in the adjustment area. A large number of safety cabinets are arranged in the adjustment area, and an operator assigned to each safety cabinet performs the adjustment work at each stage necessary for cell culture using the safety cabinet. That is, the adjustment area is an area that should be called the heart of the culture facility, and most adjustment operations necessary for cell culture are performed through a safety cabinet arranged in the adjustment area.

  The central management system is a system that manages the overall operation of the culture facility, and centrally manages all information related to equipment such as packs, centrifuge tubes, culture dishes, materials, and chemicals that enter and exit each area. In this overall management system, work instructions are issued for each safety cabinet regarding the work contents to be carried out on the current day.

  As shown in FIG. 3, the work instruction sheet displays a safety cabinet number, work date and time, operator name, cell information (cell type, provider name, collection date), work content, equipment ID list, and the like. . The equipment ID list is a list in which individual ID numbers of various equipments carried into the cabinet are displayed according to work contents. For example, when the work content is passage processing, it is necessary to carry a culture dish in which cells have been cultured as a cell transfer source from the culture area into the cabinet, and the ID of the culture dish specified for that purpose. The number is displayed.

  In the passaging treatment, a predetermined culture solution is poured into a new empty culture dish, and the cells are transferred to the medium of the new culture dish. For this reason, it is necessary to carry in a new culture dish and a pipette as a transfer tool from the material area into the cabinet. Moreover, it is necessary to carry in the said cabinet from the chemical | medical area the washing | cleaning liquid for wash | cleaning a new culture dish, and a corresponding culture liquid. Accordingly, the equipment list displays the ID numbers assigned to these new culture dishes, pipettes, washing liquid containers, and culture liquid containers.

  The work instruction sheet can include a work plan displaying work instructions. Furthermore, the destination list of each equipment after the completion of processing can be displayed on the work instruction sheet. The contents of these work instructions are transmitted to the collating means of the safety cabinet 10 and registered in advance.

  FIG. 4 is a flowchart showing a management procedure in the safety cabinet 10. In S100, the contents of the work instruction issued by the overall management system are transmitted to the verification unit of the safety cabinet 10 and registered in advance. In S110, the operator in charge is collated. That is, each operator 18 wears an IC tag storing his / her name on the wrist, and when the wrist is brought close to the insertion slot 16 of the safety cabinet 10, the name of the operator 18 is recognized by the reader 25 via the antenna 26. The The collation means collates the name of the operator 18 recognized by the reader 25 with the operator name displayed on the work instruction, and outputs the collation result to the monitor 28.

  Next, the process proceeds to S120 for inserting equipment. In this equipment insertion operation, an operator 18 inserts each equipment transported from each area such as a material area, a medicine area, and a culture area into the accommodating portion 14 of the safety cabinet 10 through the insertion port 16 one by one. Since ID numbers are assigned in advance to all the devices to be inserted, the ID numbers of the devices are recognized by the reader 25 through the antenna 26 at the moment when each device passes through the insertion port 16 (S130). The collating means collates whether or not the equipment ID number recognized by the reader 25 exists in the equipment ID list registered in advance based on the work instruction, and outputs the collation result to the monitor 28 (S140). That is, when the ID numbers of the equipment do not match, a message to that effect is displayed on the screen of the monitor 28 and a voice warning is issued. Therefore, the operator 18 performs necessary error processing (S150), and then returns to S120 and proceeds to the next equipment insertion operation (S160). As a result of the collation, when the ID numbers of the equipment match, this is displayed on the screen of the monitor 28. While confirming whether all the instruments have been inserted (S170), the same insertion operation is repeated thereafter (S180).

  When it is confirmed that all the devices displayed in the device ID list are stored in the storage unit 14 of the safety cabinet 10, the process for cell culture is started (S190). In the course of this process, the work plan included in the work instruction can be displayed on the monitor 28, and the operator 18 executes necessary processes while referring to the work plan displayed on the monitor 28 screen. Can do. When all processes are completed in S200, each equipment accommodated in the accommodating part 14 of the safety cabinet 10 is then carried out (S210).

  The destination of each equipment after processing is divided into a centrifuge area, a culture area, a medicine area, a cell storage area, a waste area, and the like. The ID number of each device taken out from the storage unit 14 by the operator 18 is recognized by the reader 25 when passing through the insertion port 16. Accordingly, the collating means collates the ID number of the equipment to be carried out recognized by the reader 25 with the destination list of each equipment displayed in the work instruction, and outputs the destination of the equipment to the monitor 28. For this reason, the destination management of each equipment can be performed reliably.

  The safety cabinet 10 is provided with warning means for issuing a warning when the height of the insertion port 16 (opening height of the shutter 12) H exceeds a set height. That is, there is a limit to the distance that the antenna 26 for the reader 25 can accurately recognize the ID number of each device, so that each device passing through the insertion port 16 is surely within the effective detection range of the antenna 26. The height H of the insertion opening 16 is held within a set height (for example, about 20 cm). When the height H of the insertion port 16 exceeds the set height, a warning is issued by a buzzer or the like, and a message to that effect is displayed on the monitor 28. Therefore, the operator 18 drives the shutter 12 based on the warning and returns the height H of the insertion port 16 to be within the set height. For this reason, the ID number of each device passing through the insertion port 16 can be accurately read by the reader 25.

  Also in the processing steps from S190 to S200 shown in FIG. 4, the operator 18 performs various processing operations by inserting both arms from the insertion port 16 into the accommodating portion 14 while holding the insertion port 16 at a certain height. Execute. In the accommodating part 14, the internal atmosphere is maintained at a negative pressure so as not to leak out of the safety cabinet 10. If the height H of the insertion port 16 is excessive, it becomes difficult to maintain the inside of the accommodating portion 14 at a negative pressure. Therefore, the above-described warning means for the height of the insertion port 16 functions effectively not only during the insertion operation of the equipment from S130 to S170 shown in FIG. 4 but also during the processing from S190 to S200.

  The safety cabinet 10 may be equipped with a reader / writer instead of the reader 25, and the work history performed in the safety cabinet 10 may be written in the IC tag of the equipment that is carried out from the insertion port 16 after the processing is completed. .

  As described above, in the safety cabinet 10 of the present embodiment, when all the devices inserted into the housing portion 14 pass through the insertion port 16, the ID number is recognized by the reader 25, and this ID number is the integrated management system. And is collated with the equipment list of the work instruction sheet registered in advance.

  For this reason, accidents related to human life such as mistakes between cell donors and cultured cells can be reliably prevented, and materials such as culture dishes, centrifuge tubes, pipettes and packs used for cell culture, and chemicals such as culture solutions and washing solutions The type and quantity can be managed precisely. Further, each device taken out from the storage unit 14 by the operator 18 after the processing is completed, the ID number is recognized by the reader 25 when passing through the insertion port 16, and the collation means outputs the destination of the device to the monitor 28. be able to. For this reason, the destination mistake of equipment decreases, and operation of an efficient culture facility can be aimed at.

  Further, since a warning is issued by a buzzer or the like when the height H of the insertion port 16 exceeds the set height, it becomes easy to manage the height H of the insertion port 16 within the set height. The ID number of each passing instrument can be read accurately by the reader 25.

  5 to 8 are perspective views showing other embodiments of the safety cabinet according to the present invention. In these drawings, elements denoted by the same reference numerals as those in FIG. 1 are elements having the same functions as those in the first embodiment, and description thereof is omitted.

  In the safety cabinet 10 </ b> A according to the second embodiment of FIG. 5, the antenna 26 </ b> A is arranged so as to enter the front side in the housing portion 14. Since the antenna 26A is provided over the entire width of the insertion slot 16, when the equipment is moved in and out of the insertion slot 16, the ID number carried on each equipment is surely recognized by the reader 25, and the collation result is displayed on the monitor 28. Can be output. According to the second embodiment, the antenna 26A is arranged so as to enter the accommodating portion 14, and since there is no protruding portion on the front outside of the safety cabinet 10A, the workability of various operations by the operator is good.

  In the safety cabinet 10 </ b> B according to the third embodiment of FIG. 6, the antenna 26 </ b> B is disposed in a coil shape at the lower end of the shutter 12. Since the antenna 26B is also provided over the entire width of the insertion slot 16, when an instrument is moved in and out of the insertion slot 16, the ID number carried by each instrument is reliably recognized by the reader 25, and the collation result is monitored 28. Can be output. Moreover, since there is no protrusion location outside the front surface of the safety cabinet 10B as in the second embodiment, the workability of various operations by the operator is good.

  In the safety cabinet 10 </ b> C according to the fourth embodiment of FIG. 7, the antenna 26 </ b> C is disposed on the entire floor of the housing portion 14. The reader 25 reads ID numbers carried on each device intermittently (for example, every 10 seconds) for all the devices stored in the storage unit 14. The collating means collates the ID number of the equipment read by the reader 25 with the equipment list of the work instruction book registered in advance, and the equipment of the ID number not displayed in the equipment list is mixed in the ID number of the read equipment. If so, the device is specified and displayed on the monitor 28 and a warning is issued. And it continues issuing a warning until the said equipment is removed from the accommodating part 14 by the operator. According to the fourth embodiment, since all the equipment accommodated in the accommodating portion 14 is intermittently verified (for example, every 10 seconds), if an inappropriate equipment enters the insertion port 16 by any chance. But you can take immediate action. Further, since the antenna 26C is arranged on the floor surface in the housing portion 14 and there is no protruding portion on the outer front side of the safety cabinet 10C, the workability of various operations by the operator is good.

  In the safety cabinet 10 </ b> D according to the fifth embodiment of FIG. 8, the antenna 26 </ b> D is disposed on the ceiling surface of the housing portion 14. In the fifth embodiment as well, since all the devices accommodated in the accommodating portion 14 are intermittently verified (for example, every 10 seconds), even if an inappropriate device enters the insertion port 16 immediately, Can take measures. In addition, since the antenna 26D is arranged on the ceiling surface in the housing portion 14 and there is no protruding portion on the outer front side of the safety cabinet 10D, workability of various operations by the operator is good.

  In each of the above-described embodiments, the case where an IC tag is used as means for supporting identification information on each device has been described. However, the means for carrying the identification information according to the present invention is not limited to this, and a bar code or a two-dimensional code can also be used.

1 is a perspective view showing a first embodiment of a safety cabinet for cell culture adjustment according to the present invention. It is explanatory drawing which showed the relationship between a safety cabinet and a comprehensive management system. It is explanatory drawing which illustrated the content of the work instruction document. It is the flowchart which showed the management procedure in the safety cabinet. It is a perspective view which shows 2nd Embodiment of the safety cabinet for cell culture adjustment which concerns on this invention. It is a perspective view which shows 3rd Embodiment of the safety cabinet for cell culture adjustment which concerns on this invention. It is a perspective view which shows 4th Embodiment of the safety cabinet for cell culture adjustment which concerns on this invention. It is a perspective view which shows 5th Embodiment of the safety cabinet for cell culture adjustment which concerns on this invention.

Explanation of symbols

  10, 10A, 10B, 10C, 10D ......... Safety cabinet, 12 ......... Shutter, 14 ......... Housing unit, 16 ......... Insertion port, 18 ......... Operator, 20 ...... Culture dish, 22 ......... Centrifuge tube, 24 ......... IC tag, 26, 26A, 26B, 26C, 26D ......... antenna, 28 ......... monitor.

Claims (6)

An insertion port for equipment used for cell culture adjustment, a receiving part for accommodating equipment inserted from the insertion opening, a reader for recognizing identification information carried by the inserted equipment at the insertion opening, and the reader A safety cabinet for cell culture adjustment , comprising: collation means for collating whether or not the identification information recognized by the identification information coincides with previously registered identification information; and output means for outputting a collation result by the collation means. ,
A safety cabinet for cell culture adjustment, comprising: an antenna for the reader whose detection range is the entire width of the insertion slot at the front surface of the insertion slot . Claims, characterized in that it comprises a warning means for the shutter for opening and closing the insertion opening in the vertical direction is provided, an alarm and opening height of the shutter is equal to or greater than the height which is set within the effective detection range of the antenna Item 2. A safety cabinet for cell culture adjustment according to Item 1 . An insertion port for equipment used for cell culture adjustment, a receiving part for accommodating equipment inserted from the insertion opening, a reader for recognizing identification information carried by the inserted equipment at the insertion opening, and the reader A safety cabinet for cell culture adjustment , comprising: collation means for collating whether or not the identification information recognized by the identification information coincides with previously registered identification information; and output means for outputting a collation result by the collation means. ,
A safety cabinet for cell culture adjustment , comprising a shutter for opening and closing the insertion port in the vertical direction and an antenna installed at the lower end of the shutter . A storage unit for storing equipment used for cell culture adjustment, a reader for recognizing identification information carried by each of the plurality of equipments stored in the storage unit, and each identification information recognized by the reader in advance A safety cabinet for cell culture adjustment , comprising: a collating unit that collates whether or not the registered identification information is matched; and an output unit that outputs a collation result by the collating unit. A safety cabinet for cell culture adjustment, comprising an antenna for the reader whose detection range is the entire width of the front part of the surface. A storage unit for storing equipment used for cell culture adjustment, a reader for recognizing identification information carried by each of the plurality of equipments stored in the storage unit, and each identification information recognized by the reader in advance A safety cabinet for cell culture adjustment , comprising: a collating unit that collates whether or not the registered identification information is matched; and an output unit that outputs a collation result by the collating unit. A safety cabinet for cell culture adjustment , wherein the reader antenna is installed on the entire surface . A storage unit for storing equipment used for cell culture adjustment, a reader for recognizing identification information carried by each of the plurality of equipments stored in the storage unit, and each identification information recognized by the reader in advance A safety cabinet for cell culture adjustment , comprising: a collating means for collating whether or not it matches registered identification information; and an output means for outputting a collation result by the collating means , wherein the ceiling surface of the accommodating portion A safety cabinet for cell culture adjustment , characterized in that the reader antenna is installed .

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