JP4452704B2 - 抗真菌剤の有効性の鑑別法 - Google Patents
抗真菌剤の有効性の鑑別法 Download PDFInfo
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- JP4452704B2 JP4452704B2 JP2006192248A JP2006192248A JP4452704B2 JP 4452704 B2 JP4452704 B2 JP 4452704B2 JP 2006192248 A JP2006192248 A JP 2006192248A JP 2006192248 A JP2006192248 A JP 2006192248A JP 4452704 B2 JP4452704 B2 JP 4452704B2
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Description
(2)前記組織が爪又は皮膚であることを特徴とする、(1)に記載の鑑別法。
(3)真菌の生育を、爪組織の薄片又は皮膚組織の薄片における真菌の性状が菌糸を形成しているか、分生子の状態であるかで判定することを特徴とする、(1)又は(2)に記載の鑑別法。
(4)更に、真菌を播種した組織の薄片において、真菌の生育抑制が認められた場合、培地に移植した組織の薄片を、真菌の生育状況の判定後、リン脂質とポリオキシエチレンソルビタン脂肪酸エステルとを含む培地に再度移植し、前記組織の薄片より真菌が生育するか否かを調べ、生育する場合には前記有効濃度は静菌的有効濃度であると鑑別し、生育しない場合には殺菌的有効濃度であると鑑別することを特徴とする、(1)〜(3)の何れか1項に記載の鑑別法。
Claims (4)
- 動物の組織に抗真菌剤を投与し、しかる後に抗真菌剤の作用及び/又は標的部位から、組織に対して一定方向に切り出した複数の薄片のうち、同一性の高い2枚以上ずつの薄片のうち一部の薄片については、該薄片より溶剤によって抽出される抗真菌剤の濃度を測定して夫々の薄片での抗真菌剤の定量値を得、一方、前記2枚以上の薄片のうちの他の一部の薄片については、該薄片に真菌を播種して培地に移植し、該薄片における真菌の生育に対する作用を鑑別して得られる抗真菌活性を得、かかる抗真菌活性と前記定量値とを対照させることにより、抗真菌剤の有効濃度を鑑別する方法。
- 組織が、爪又は皮膚である、請求項1に記載の方法。
- 真菌の生育に対する作用を、爪組織の薄片又は皮膚組織の薄片における真菌の性状が、菌糸を形成しているか又は分生子の状態であるかにより判定する、請求項1又は2に記載の方法。
- 培地に移植した真菌を播種した薄片において、真菌の生育抑制が認められた場合、さらに該薄片を、リン脂質及びポリオキシエチレンソルビタン脂肪酸エステルとを含む培地に再度移植し、真菌が生育する場合には抗真菌剤の濃度が静菌的有効濃度であり、生育しない場合には殺菌的有効濃度であると鑑別する、請求項1〜3のいずれかに記載の方法。
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JP2006192248A JP4452704B2 (ja) | 2005-08-31 | 2006-07-13 | 抗真菌剤の有効性の鑑別法 |
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JP2007093584A JP2007093584A (ja) | 2007-04-12 |
JP2007093584A5 JP2007093584A5 (ja) | 2008-01-10 |
JP4452704B2 true JP4452704B2 (ja) | 2010-04-21 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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JP5458368B2 (ja) * | 2008-12-18 | 2014-04-02 | 株式会社ポーラファルマ | 爪白癬モデルの製造法 |
JP2010142164A (ja) * | 2008-12-19 | 2010-07-01 | Pola Pharma Inc | 爪白癬モデルの製造法 |
CN104237414B (zh) * | 2014-09-22 | 2016-08-24 | 衢州出入境检验检疫局综合技术服务中心 | 液质联用仪同时检测柑橘中多种防腐保鲜剂残留的方法 |
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