JP4402593B2 - Liquid enzyme for contact lenses - Google Patents

Description

  The present invention relates to a liquid enzyme agent for contact lenses. More particularly, the present invention relates to a liquid enzyme agent that is added to a treatment solution such as a contact lens cleaning preservative, a bactericide, and a cleaning preservative bactericide to remove contact lens dirt by contacting the contact lens.

  In order to remove protein stains adhering to contact lenses, decomposition removal by a proteolytic enzyme is generally performed. As a means of stabilizing the proteolytic enzyme, a method of forming an effective amount of the proteolytic enzyme into a solid form such as a tablet, granule or powder is taken. There was a problem that time was needed. In order to solve such problems, liquid proteolytic enzyme agents have also been developed. For example, Patent Document 1 and Patent Document 2 describe a liquid enzyme agent comprising a low-molecular organic liquid such as glycerin, propylene glycol, or polyethylene glycol and a proteolytic enzyme. In such conventional techniques, many combinations of water-soluble low-molecular organic liquids and proteolytic enzymes were observed, and water-soluble low-molecular organic liquids were clearly recognized as main components for enzyme stabilization. However, when a hydrous soft contact lens is treated with an enzyme agent in which a proteolytic enzyme is stabilized by a water-soluble low-molecular organic liquid, the water-soluble low-molecular organic liquid contained in the treatment liquid is taken into the lens, and the lens This causes the problem of expanding and deforming. In order to avoid this problem, in some proteolytic enzyme agents on the market, it is stated in the instruction manual that one use amount is one drop, but several drops are used by mistake. There are many cases.

  In addition, since the osmotic pressure of the treatment liquid dripped with a proteolytic enzyme agent composed mainly of a water-soluble low-molecular-weight organic liquid becomes hypertonic, it may affect the lens and irritation to ocular tissues during misuse. There are concerns.

Japanese Patent Laid-Open No. 1-180515 Japanese Patent Laid-Open No. 2-168224

  In view of the prior art, an object of the present invention is to provide a liquid enzyme agent for contact lenses that does not adversely affect lenses and eye tissues.

  The present invention relates to a liquid enzyme agent for contact lenses comprising a water-soluble polymeric organic solid having an average molecular weight of 800 to 100,000, a viscosity modifier and an enzyme.

  In the contact lens liquid enzyme agent, the water-soluble polymer organic solid is preferably a polyalkylene glycol.

  In the contact lens liquid enzyme agent, the water-soluble polymer organic solid is preferably polyethylene glycol or polyethylene glycol monomethyl ether.

  In the contact lens liquid enzyme agent, the viscosity modifier is preferably a water-soluble low-molecular organic liquid.

  The liquid enzyme agent for contact lens preferably contains 5 to 45 w / w% of a water-soluble polymer organic solid.

  The liquid enzyme agent for contact lenses preferably contains 15 to 45 w / w% of a viscosity modifier.

  The water-soluble polymer organic solid contained in the liquid enzyme agent for contact lens of the present invention is not taken into the contact lens and does not adversely affect the lens such as swelling. Therefore, the liquid enzyme agent for contact lens of the present invention does not impair the lens function. Moreover, the liquid enzyme agent for contact lenses of this invention can prevent the raise of the process liquid osmotic pressure at the time of dripping an enzyme agent, and can suppress the bad influence to a lens, and the eye irritation at the time of misuse. Furthermore, the liquid enzyme agent for contact lenses of the present invention has the same enzyme activity stabilizing action as the conventional liquid enzyme agent for contact lenses (water-soluble low molecular organic liquid + proteolytic enzyme).

  As described above, the liquid enzyme agent for contact lenses of the present invention has a very excellent effect.

  The liquid enzyme agent for contact lenses of the present invention contains a water-soluble polymer organic solid having an average molecular weight of 800 to 100,000, a viscosity modifier and an enzyme as essential components.

  The enzymes contained in the liquid enzyme agent for contact lenses of the present invention include those that degrade proteins that are the main components of contact lens stains, and those that degrade other stain components. Specific examples include, but are not limited to, proteolytic enzymes, lipolytic enzymes and / or glycolytic enzymes.

  The water-soluble polymer organic solid used in the present invention means a water-soluble synthetic polymer organic solid which is a flowable solid (such as petrolatum) or a solid and does not have micelle forming ability. Accordingly, natural products such as proteins, celluloses and polysaccharides are not included herein in the water soluble polymeric organic solids.

  The water-soluble polymer organic solid used in the present invention is not particularly limited as long as it is a water-soluble synthetic polymer organic solid having an average molecular weight of 800 to 100,000, preferably 1,000 to 20,000. When the average molecular weight is less than 800, the characteristic water retention effect tends not to be exhibited, and when it exceeds 100,000, the viscosity when the water-soluble polymer organic solid is dissolved tends to be too high. The water-soluble polymer organic solid is considered to contribute to enzyme stabilization by suppressing the hydrolysis reaction of the proteolytic enzyme due to the water retention effect of the polymer chain.

  As the water-soluble polymer organic solid in the liquid enzyme agent for contact lenses of the present invention, those having a very high solubility in water (for example, those capable of dissolving 15 w / w% or more in 20 ° C. water) are preferable, and It is more preferable that the viscosity of the solution does not become too high (for example, 10 Pa · S) even when a large part by weight is dissolved. A water-soluble polymer organic solid having a liquid pH after dissolution in a neutral region is also preferable.

  In the liquid enzyme agent for contact lenses of the present invention, the water-soluble polymer organic solid includes, for example, polyethylene glycol, polypropylene glycol, and poly (ethylene glycol monomethyl ether) in which a methyl group is ether-bonded to one of the terminal hydroxyl groups of polyethylene glycol. Alkylene glycols, polyvinyl caprolactams such as polyvinyl pyrrolidone and poly N-vinyl caprolactam, polyethers such as crown ether, polyacrylamides such as polyacrylamide hydrochloride, polydimethylacrylamide and polydiethylacrylamide, polyhydroxyethyl methacrylate, etc. A hydroxyl group-containing (meth) acrylate or a polyol such as polyvinyl alcohol can be used. Among water-soluble polymer organic solids, polyalkylene glycols are preferable from the viewpoints of economy, solubility in water, viscosity of liquid enzyme agent, safety, color, and the like, and among them, polyethylene glycol and polyethylene glycol monomethyl ether are more preferable. Among them, those having an average molecular weight of 1000 or more are most preferable.

  The blending amount of the water-soluble polymer organic solid in the contact lens liquid enzyme agent of the present invention is preferably 5 to 45 w / w%, more preferably 5 to 20 w / w%. When the blending amount of the water-soluble polymer organic solid is less than 5 w / w%, there is a tendency that the enzyme stability, the osmotic pressure reducing effect, and the lens deformation preventing effect are not sufficiently obtained. If it exceeds 45 w / w%, organic solids tend to precipitate when the saturation solubility is reached due to temperature changes or the like, and the viscosity tends to be too high.

  The viscosity modifier used in the present invention can be used together with a water-soluble polymer organic solid to reduce the viscosity of the enzyme agent compared to the case where the stability of the enzyme is achieved with only the water-soluble polymer organic solid. It is. That is, the viscosity modifier has a function of facilitating the dripping of the liquid enzyme agent by adding it to the liquid enzyme agent for contact lenses, and facilitating the mixing of the liquid enzyme agent after the dripping with the washing preservation solution. However, if the enzyme is stabilized with only a viscosity modifier, the viscosity of the enzyme agent can be kept low, but the osmotic pressure of the treatment liquid mixed with the washing preservation solution becomes hypertonic, which affects the lens and causes misuse. There are also concerns about irritation to the eye tissue. Therefore, in the liquid enzyme agent in the present invention, it is important to use a water-soluble polymer organic solid and a viscosity modifier in combination. Specific examples of such viscosity modifiers include water-soluble low molecular organic liquids.

  The low molecular organic liquid means an organic liquid that is fluid at normal temperature and normal pressure. In the liquid enzyme agent for contact lenses of the present invention, for example, glycerin, propylene glycol, low molecular polypropylene glycol, ethylene glycol, diethylene glycol and the like can be used as the low molecular organic liquid. Among the low-molecular organic liquids, glycerin and propylene glycol are preferable from the viewpoint of the enzyme stability effect, the influence on the lens, the economical aspect, and the performance as a food additive.

  The blending amount of the viscosity adjusting agent in the liquid enzyme agent for contact lenses of the present invention is preferably 15 to 45 w / w%, more preferably 20 to 40 w / w%. If the blending amount of the viscosity modifier is less than 15 w / w%, the viscosity of the enzyme agent may not be kept low. If it exceeds 45 w / w%, the osmotic pressure after the treatment is increased, or the lens May be deformed.

  The liquid enzyme agent for contact lenses of the present invention may contain calcium. Calcium contributes to the stabilization of proteolytic enzymes. The blending amount of calcium in the contact lens liquid enzyme of the present invention is preferably 0.005 to 0.05 w / w%, more preferably 0.03 w / w%. When the amount is less than 0.005 w / w%, a sufficient enzyme stability effect tends not to be obtained. When the amount exceeds 0.05 w / w%, the enzyme tends to be destabilized.

  The liquid enzyme agent for contact lenses of the present invention may contain known auxiliaries such as preservatives, bactericides, pH adjusters (buffering agents), and / or surfactants. These auxiliaries allow the enzyme to be stably present in the liquid enzyme agent for contact lenses of the present invention or the final preparation of the enzyme agent, and adversely affect the target, human body and environment to which the enzyme agent of the present invention is applied. There is no particular limitation as long as it does not. Moreover, the addition amount of each adjuvant is not limited to the following illustrations, It can set suitably by those skilled in the art.

  Examples of the preservatives include mercury preservatives such as phenylmercuric nitrate, phenylmercuric acetate and thimerosal, surfactant preservatives such as benzalkonium chloride and pyridinium bromide, chlorhexidine, polyhexamethylene biguanide and chlorobutanol. Alcohol-based preservatives such as methylparaben, propylparaben, dimethyloldimethylhydantoin, imidazolium urea, boric acid, boric acid compounds or borax can be used. Among them, preferred is one or two or more substances selected from boric acid, boric acid compounds and borax, and the addition amount is 0.00001 to 3.0 w / w%. Preferably there is.

  As the bactericidal agent, an organic nitrogen type bactericidal agent is preferable because it has a high bactericidal effect at a low concentration. Among them, a biguanide compound or a derivative thereof (polymer or salt) is more preferable because it is highly safe. Polyhexamethylene biguanide (PHMB) is very preferable because it does not absorb or adsorb.

  Representative examples of the organic nitrogen-based disinfectant include (1) quaternary ammonium compound or a polymer thereof, such as benzalkonium chloride, benzethonium chloride, and dimer 136, and (2) a biguanide compound or a polymer thereof or the like. Examples thereof include chlorhexidine gluconate and polyhexamethylene biguanide which are salts, and (3) polymers of the above (1) and (2). The amount of the organic nitrogen-based fungicide added is about 0.000001 to 10 w / w%, preferably about 0.00001 to 1 w / w%.

  Examples of the pH adjuster (buffering agent) include boric acid and its sodium salt, phosphoric acid and its sodium salt, citric acid and its sodium salt, lactic acid and its sodium salt, glycine or glutamine, and other amino acids and their sodium salts. Or malic acid and its sodium salt can be used. The addition amount of the pH adjuster is preferably 0.001 to 3.0 w / w%.

  As the surfactant, for example, any surfactant such as an anionic surfactant, a nonionic surfactant, or an anionic surfactant and a nonionic surfactant may be used.

  Examples of the anionic surfactant include sodium alkyl sulfate, sodium alkylbenzene sulfonate, sodium alkyloylmethyl taurate, sodium alkyloyl sarcosine, sodium α-olefin sulfonate, sodium polyoxyethylene alkyl ether phosphate, polyoxyethylene alkyl Sodium ether sulfate, polyoxyethylene alkylphenyl ether sodium sulfate, di (polyoxyethylene alkyl ether) sodium phosphate, and the like can be used. The amount of the anionic surfactant added is, for example, 0.01 w / v% or more, preferably 0.02 w / v% or more, and 10 w / v% or less, preferably 5 w / v% or less.

  Nonionic surfactants include, for example, polyethylene glycol adducts of higher alkylamines, polyethylene glycol adducts of higher fatty acid amides, polyglycerol esters of higher fatty acids, polyethylene glycol esters of higher fatty acids, polyalkylene glycols of higher fatty acids, polyethylene Glycol copolymer ester, polyhydric alcohol ester with higher fatty acid polyethylene glycol added, polyethylene glycol ether of higher alcohol, polyglycerin ether of higher alcohol, polyethylene glycol ether of alkylphenol, formaldehyde condensate of polyethylene glycol ether of alkylene phenol, polypropylene glycol -Polyethylene glycol copolymer, phosphate ester, castor oil Hydrogenated castor oil, polyethylene glycol sorbitan alkyl esters, polyethylene glycol adducts of sterols like can be used. The addition amount of the nonionic surfactant is, for example, 0.01 w / v% or more, preferably 0.02 w / v% or more, and 10 w / v% or less, preferably 5 w / v% or less.

  When the anionic surfactant and the nonionic surfactant are used in combination, the content of the anionic surfactant and the nonionic surfactant is within the above-described range, and the total amount is 0.02 to 20 w / v%, preferably 0.05 to 10 w / v%.

  In addition, you may add a pigment | dye to the liquid enzyme agent for contact lenses of this invention.

  The liquid enzyme agent for contact lens of the present invention is used after preparing a diluted solution by dropping it on a multipurpose solution for contact lens. The multi-purpose solution means a liquid agent that can store, wash, disinfect, and rinse contact lenses in one liquid, including a contact lens cleaning and preserving solution, a bactericidal agent, and a cleaning and preserving bactericidal agent (for example, , Ines (sales agency: Menicon Co., Ltd.)). By immersing the contact lens in the diluted solution for a certain period of time, dirt components such as proteins adhering to the contact lens can be removed without rubbing.

  Hereinafter, although the liquid enzyme agent for contact lenses of this invention is demonstrated in detail by an Example, this invention is not limited only to this Example.

Examples 1-12, Comparative Examples 1-16
Liquid enzyme agents for contact lenses of the present invention (Examples 1 to 12), conventional enzyme agents (Comparative Examples 1 to 11) consisting only of water-soluble low-molecular organic liquids and proteolytic enzymes, water-soluble low-molecular organic liquids and , An enzyme agent comprising a water-soluble polymer organic solid and a proteolytic enzyme outside the predetermined content of the present invention (Comparative Examples 12 and 13) and an enzyme agent comprising only a water-soluble polymer organic solid and a proteolytic enzyme (comparison) Examples 14-16) were prepared with the compositions described in Tables 1 and 2.

In Tables 1 and 2, * 1 to * 7 indicate the following.
* 1: Polyethylene glycol (average molecular weight about 200, liquid, manufactured by Nacalai Tesque)
* 2: Polyethylene glycol (average molecular weight about 1000, solid, manufactured by Nacalai Tesque)
* 3: Polyethylene glycol (average molecular weight about 4000, solid, manufactured by Nacalai Tesque)
* 4: Polyethylene glycol monomethyl ether (average molecular weight about 2000, solid, manufactured by Aldrich Chemical Company Inc.)
* 5: Polyvinylpyrrolidone (average molecular weight about 10,000, solid, manufactured by Tokyo Chemical Industry Co., Ltd.)
* 6: ClearLens-Pro2.0L (Novoenzyme)
* 7: CL-5PG (manufactured by Nagase ChemteX Corporation)

  In addition, * 1 to * 4 used a titration method, and * 5 used a raw material whose average molecular weight was determined by a viscosity method.

  For each enzyme agent, a residual activity measurement of proteolytic enzyme, an osmotic pressure measurement test in a multi-purpose solution, and a contact lens size change measurement test were performed. Moreover, the preservation | save efficacy test was also implemented about Examples 11, 12 and Comparative Examples 8-13.

Test Example 1 (Measurement of residual activity of proteolytic enzyme)
The residual activity of the proteolytic enzyme in each of the enzyme agents of Examples 1 to 12 and Comparative Examples 1 to 7 and 14 to 16 was measured after 1 week and 2 weeks from the preparation of the enzyme agent. However, the enzyme agents of Examples 11 and 12 were measured one week and one month after the preparation of the enzyme agent. The measurement was performed as follows.

Mix 4 mL of 2.0% casein solution (pH 8, 0.05 M Tris-sodium hydroxide aqueous solution) heated to 37 ° C. with 3.6 mL of diluent (pH 8, 10 mM Tris-hydrochloric acid aqueous solution) and 0.4 mL of enzyme preparation. did. Immediately after mixing, 1 mL of the mixture was added to 1 mL of 0.4 M trichloroacetic acid aqueous solution and mixed to precipitate undegraded casein. This operation was performed three times to obtain three samples (n = 3). The initial absorption value at 280 nm by the casein degradation product in each supernatant was measured, and the average value A ₀ of the three initial absorption measurement results was determined. The remaining 5 mL was kept at 37 ° C. for 10 minutes, and 1 mL thereof was used to precipitate undegraded casein by the precipitation method. This operation was performed four times to obtain four samples (n = 4). The absorption value after the process in 280 nm in each supernatant liquid was measured, and the average value A of four absorption results after the process was obtained. The value obtained by subtracting the initial absorption average value A ₀ from the post-treatment absorption average value A was defined as the activity value of the proteolytic enzyme.

The residual activity (%) was calculated by the following formula.
Residual activity value (%) = [(A−A ₀ ) / A _Day0 ] × 100
A _Day0 : Enzyme activity value on the test start date

  Tables 3 and 4 show the residual activity values of the enzymes in each enzyme agent.

  As is apparent from Tables 3 and 4, the water-soluble polymer organic solid exhibited a sufficient enzyme activity stabilizing action, and was found to be almost equivalent to the enzyme activity stabilizing action of the water-soluble low-molecular organic liquid. It has also been found that a sufficient stabilizing effect can be obtained even when a water-soluble high-molecular organic solid and a water-soluble low-molecular organic liquid are mixed in various proportions.

Test example 2 (osmotic pressure measurement test in multi-purpose solution)
The enzyme agents for contact lenses of Examples 1 to 9, 11 and 12, and Comparative Examples 1 to 7 and 14 to 16 were added to the multipurpose solution, respectively, and the osmotic pressure of the solutions was measured.

  As a multi-purpose solution, Ines (sales company: Menicon Co., Ltd.) was used, and 80 μL (2 drops equivalent amount) of each enzyme agent was added to 2 mL of Ines. For the osmotic pressure measurement, HOSM-1 (manufactured by TOA Electronics Ltd.) was used.

  The osmotic pressure of each solution is shown in Tables 5 and 6.

  The enzyme agent is diluted 26-fold (80 μL / 2 mL) and is small when viewed from the total amount. As is clear from Tables 5 and 6, when a large amount of propylene glycol or glycerin is contained, the osmotic pressure is significantly high. It was confirmed that On the other hand, when a large amount of water-soluble polymer organic solid was contained, the osmotic pressure was clearly lower than those.

Test Example 3 (Contact lens DIA change measurement test)
The degree of swelling of the lens when immersed in each enzyme agent for a certain period was measured. The measurement was performed as follows.

  A treatment solution was prepared by mixing 80 μL of the enzyme agent (2 drops equivalent amount) with 2 mL of Ines. Subsequently, the contact lens was immersed in each processing solution. For the enzyme agents of Example 3 and Comparative Example 1, 1 day and 3 days after immersion, and for the enzyme agents of Examples 11 and 12 and Comparative Examples 3, 4 and 5, 1 day and 4 days after immersion. In addition, for the enzyme agents of Examples 1, 2, 4, 5, 6, 7, 8, and 9 and Comparative Examples 2, 6, 7, 14, 15, and 16, contact was made 1 day and 5 days after immersion. The lens diameter (DIA) was measured. In the test, frequent exchange lens Accuview 2 (manufactured by Johnson & Johnson Co., Ltd., water content 58%) was used, and the treatment solution was changed once a day. In the DIA measurement, the diameter (A) is measured using the marking attached to the contact lens as an index, and then the diameter (B) perpendicular to the diameter (A) is measured. The average value of the diameter (A) and the diameter (B) was taken as the DIA measurement value.

  Tables 7 and 8 show the results of comparing the DIA measurement values of the lens after treatment with those before treatment.

  As is clear from Tables 7 and 8, it was found that the lens swells (deforms) significantly when it contains a large amount of water-soluble low molecular organic liquid. However, it has been found that when a large amount of the water-soluble polymer organic solid is contained, it does not swell (deform). Interestingly, polyethylene glycol (liquid) having an average molecular weight of about 200 swells (deforms) the lens, but it has been found that the deformation can be suppressed when the average molecular weight is 1000 or more.

Test Example 4 (Viscosity comparison test of liquid enzyme agent for contact lens)
Each enzyme agent of Examples 1-9, 11 and 12 and Comparative Examples 1-7 and 14-16 was put into a glass container and capped. Subsequently, the falling state of the enzyme solution when the container was turned upside down was visually observed, and the viscosity of each enzyme solution was compared. The results are shown in Tables 9 and 10.

○ 容器を逆さまにした場合、直ちに落下した酵素溶液。
△ 容器を逆さまにした場合、１秒以内に容器を伝わって落下した酵素溶液。
× 容器を逆さまにした場合、１秒経過しても落下しない酵素溶液。

○ If the container is turned upside down, the enzyme solution dropped immediately.
△ When the container is turned upside down, the enzyme solution that fell through the container within 1 second.
× Enzyme solution that does not fall even after 1 second when the container is turned upside down.

<Comprehensive evaluation>
Regarding Examples 1 to 9, 11 and 12, and Comparative Examples 1 to 7 and 14 to 16, the effects of each enzyme agent were comprehensively evaluated based on the results of Test Examples 1 to 4. Each evaluation is shown in Tables 11 and 12.

  In Tables 11 and 12, (a), (b), (c) and (d) and symbols are as follows.

In addition, in common with each of the items (a) to (d), each symbol is: ○: preferable as a liquid enzyme agent for contact lenses, Δ: extent that can be used as a liquid enzyme agent for contact lenses, ×: contact It is unsuitable as a liquid enzyme agent for lenses.
(A) Measured degree of lens swelling (based on the results of Tables 7 and 8)
○ Change in size before and after lens immersion is 0.05 mm or less △ Change in size before and after lens immersion is greater than 0.05 mm and less than 0.10 × Change in size before and after lens immersion is greater than 0.10 mm (ii) Osmotic pressure Measured rise (based on results in Tables 5 and 6)
○ The osmotic pressure is 450 mOsm / kg or less △ The osmotic pressure is larger than 450 mOsm / kg and less than 500 mOsm / kg × The osmotic pressure is larger than 500 mOsm / kg (U) Measurement of enzyme activity stabilization after 2 weeks or 1 month (Based on the results in Tables 3 and 4)
○ 80% or more △ 60% or more and less than 80% × less than 60% (iv) Viscosity comparison (based on the results of Tables 9 and 10)
○ If the container is turned upside down, the enzyme solution dropped immediately.
△ When the container is turned upside down, the enzyme solution that fell through the container within 1 second.
× Enzyme solution that does not fall even after 1 second when the container is turned upside down.

<Evaluation criteria for comprehensive evaluation>
◎ When all items are ○. The corresponding enzyme agent is very preferable as a liquid enzyme agent for contact lenses.
○ When at least one item is Δ and the remaining items are ○. The corresponding enzyme agent is preferable as a liquid enzyme agent for contact lenses.
X When at least one item is x. The corresponding enzyme agent is unsuitable as a liquid enzyme agent for contact lenses.

  From the results of Test Examples 1 to 4 and the comprehensive evaluation shown in Tables 11 and 12, it was confirmed that the examples were effective as liquid enzyme agents for contact lenses. Here, in order to confirm the further effectiveness of an Example, the following test was implemented using the enzyme agent of Examples 11 and 12 and Comparative Examples 8-13.

Test Example 5 (preservation efficacy test)
Five types of bacterial suspensions were prepared as test bacteria: Pseudomonas aeruginosa IFO 13275, Staphylococcus aureus IFO 13276, E. coli IFO 3972, Candida albicans IFO 1594, and Aspergillus niger ATCC 16404. As test samples, the enzyme agents of Examples 11 and 12 and Comparative Examples 8 to 13 were used. 100 μL each of the bacterial suspension was inoculated into 10 mL of the test sample dispensed into the tube and mixed. Each test sample inoculated with the fungus was placed in a 22 ° C. incubator and cultured. In order to measure the number of inoculated bacteria, physiological saline inoculated with bacteria (separately prepared) is diluted 10 times with physiological saline, and Aspergillus niger is smeared on the medium (bacteria: SCDLP, fungus: SDLP). Was mixed. The SCDLP medium was cultured at 32 ± 2 ° C. for 6 days, and the SDLP medium was cultured at 22 ± 2 ° C. for 5 days (three per group). On the 14th day after inoculation, 0.5 mL was collected from the test sample inoculated with the fungus and diluted 10-fold with physiological saline to measure the number of viable bacteria. Was smeared and otherwise poured. The SCDLP medium was cultured at 32 ± 2 ° C. for 6 days, and the SDLP medium was cultured at 22 ± 2 ° C. for 5 days (three per group). After completion of the culture, the number of colonies was counted, and the number of viable bacteria in the sample was calculated from the average value of the three pieces. However, for bacteria, plates of 300 cfu / plate or less were counted, and for fungi, plates of 100 cfu / plate or less were counted.

  From the number of inoculated bacteria and the number of viable bacteria on the 14th day, log reduction = log (the number of inoculated bacteria / the number of bacteria on the 14th day) was obtained and used as the storage efficacy. The results are shown in Table 13.

  As is clear from Table 13, Examples 11 and 12 have a log reduction of 4 or more for all bacteria and 1 or more for all fungi, and are confirmed to have excellent storage efficacy as a preparation. It was. On the other hand, in Comparative Examples 8 to 13, none of the log reductions of 4 or more for all bacteria and 1 or more for all fungi were obtained as in Examples 11 and 12. The biggest difference in composition between Example and Comparative Example in Test Example 5 is the blending amount of PEG # 1000, and there is a very high possibility that the blending amount of PEG # 1000 contributed to the storage efficacy of the preparation. Obtained.

Claims (3)

A liquid enzyme agent for contact lenses, comprising 5 to 45 w / w% of a water-soluble polymer organic solid that is polyethylene glycol or polyethylene glycol monomethyl ether having an average molecular weight of 800 to 100,000, a viscosity modifier and an enzyme. The liquid enzyme agent for contact lenses according to claim 1, wherein the viscosity modifier is a water-soluble low molecular organic liquid. The liquid enzyme agent for contact lenses according to claim 1, comprising 15 to 45 w / w% of the viscosity modifier.