JP4370956B2 - Method and apparatus for processing soluble organic substance-containing liquid - Google Patents

Description

  The present invention is a method for treating a solution containing a soluble organic substance that can be suitably employed for treating a liquid containing a soluble organic substance such as industrial wastewater and domestic wastewater discharged from a food factory by the membrane separation activated sludge method. And a processing device.

  In the membrane separation activated sludge method applied to the treatment of a solution containing a soluble organic substance such as waste water, various phenomena that impair the treatment stability are known. For example, membrane permeability failure due to clogging of the membrane, severe foaming, scum generation, deterioration of aeration efficiency due to increase in viscosity, sludge fluidity reduction, defects due to bubble inclusion, and the like can be mentioned.

  On the other hand, various methods for predicting and grasping instability have been proposed in biological treatment of dissolved organic matter-containing liquids such as the activated sludge method. For example, dissolved oxygen, redox potential, pH fluctuation, etc. Monitoring methods are known. Particularly in recent years, a technique for monitoring the growth of useful microorganisms and helping to control the growth has been proposed (Patent Document 1). Similarly, a technique for monitoring the growth of trouble-causing microorganisms has also been proposed. For example, filamentous bacteria (“Eikel boom type”), which is one of the causes of sedimentation failure in the activated sludge process (filamentous bulking). [021N (Eikelboom Type021N) "bacteria, etc.) has been proposed (Patent Document 2).

However, such conventional methods are not applicable to all biological treatment methods of liquids containing soluble organic substances, and are often not suitable particularly in the membrane separation activated sludge method. This is because, in general, the activated sludge method including the method described in Patent Document 2 is a treatment method. Therefore, it is important to focus on countermeasures against sedimentation defects such as reduced specific gravity and reduced compactness of sludge. This is because, unlike the activated sludge method, other factors such as poor membrane water permeability, foaming, scum generation, and increased viscosity become major problems in the membrane separation activated sludge method. Nevertheless, the instability prediction and grasping method and its control method appropriate for the membrane separation activated sludge method have not been sufficiently established. Patent Document 3 and Patent Document 4 show a method of controlling the operation based on the result of measuring the viscosity of the sludge mixed solution. In this method, however, the operating condition is controlled after the viscosity has increased. It will be dealt with after actually affecting the membrane permeability, and it cannot be said that the follow-up deal is sufficient. Therefore, there is an urgent need to develop means that can predict and detect troubles and take countermeasures at an early stage in the membrane separation activated sludge method.
JP 2001-128678 A JP 2002-119300 A JP-A-7-75782 JP-A-9-75938

  The present invention has been made in response to the above-mentioned problems in the prior art, and its purpose is to use a soluble organic substance-containing liquid in a membrane-separated activated sludge method, resulting in poor membrane permeability, foaming, occurrence of scum, increased viscosity. It is an object of the present invention to provide a method and apparatus for processing while preventing the above.

In order to solve the above problems, the present invention has the following configuration. That is,
(1) When processing the soluble organic substance-containing liquid by the membrane-separated activated sludge method, while monitoring the state of the Flectobacillus strain group bacteria present in the mixed liquid of the soluble organic substance-containing liquid and the activated sludge A method for treating a soluble organic substance-containing liquid, which comprises performing a treatment.
(2) When processing the soluble organic substance-containing liquid by the membrane-separated activated sludge method, the condition of the Fructobacillus strain group bacteria present in the mixed liquid of the soluble organic substance-containing liquid and the activated sludge is monitored to determine the processing conditions. A method for treating a soluble organic substance-containing liquid, which comprises controlling.
(3) The method for treating a soluble organic substance-containing liquid according to (1) or (2) above, wherein the state of the bacteria of the Streptobacillus strain group is monitored based on genetic information of the bacteria of the Streptobacillus strain group.
(4) Any of the above (1) to (3), wherein the state of the bacteria of the Streptococcus group is monitored based on at least one of the RNA transcription characteristics, protein translation characteristics and product characteristics of the bacteria The processing method of the soluble organic substance containing liquid as described in 2.
(5) The method for treating a solution containing a soluble organic substance according to any one of the above (1) to (4), wherein the state of the bacteria of the family Flectobacillus is monitored based on the morphological characteristics of the bacteria of the family Flectobacillus. .
(6) A biological reaction tank that treats a soluble organic substance-containing liquid with activated sludge, a separation membrane that separates a mixed liquid of the soluble organic substance-containing liquid and activated sludge, and a soluble organic substance-containing liquid and activated sludge. A processing apparatus for a solution containing a soluble organic substance, comprising: a monitoring means for monitoring the state of the bacteria of the group of Streptococcus in the mixed solution.
(7) The monitoring means monitors the state of the bacteria of the Streptococcus group based on at least one of genetic information, RNA transcription characteristics, protein translation characteristics, product characteristics and morphological characteristics of the bacteria. The processing apparatus of the soluble organic substance containing liquid as described in said (6) which is what.
(8) The processing apparatus for a soluble organic substance-containing liquid according to (6) or (7), wherein the monitoring means includes a processing condition control means.

  Here, in the present invention, the membrane separation activated sludge method is a method using membrane separation to remove organic substances in waste water and pollutants such as nitrogen and phosphorus by microorganisms such as activated sludge to obtain a clear treatment liquid. I mean. The membrane separation method is not particularly limited, such as an immersion membrane method or an external membrane separation method.

  Further, in the present invention, the Flectobacillus family bacteria means that the 16S ribosomal RNA gene, which is often used as a prokaryotic phylogenetic index, has a homology of 88% or more with respect to the base sequence described in SEQ ID NO: 1. It does not matter whether or not the culture is possible. Bacteria contained in the group of bacteria belonging to the family Flectobacillus include, for example, bacteria belonging to the genus Flectobacillus, such as Flectobacillus major, Flectobacillus speluncae, Flectobacillus sp. EP293, Flectobacillus sp. Str. Etc. Naturally, even if the bacteria are not classified and are not classified as belonging to the genus Flectobacillus, if they meet the above-mentioned conditions, they are considered to be a Flectobacillus strain group bacteria because of their closeness.

  And, the state of the Flectobacillus strain group bacteria refers to the number, concentration, dominance, characteristics, etc. of the Flectobacillus strain group bacteria.

  According to the present invention, when a soluble organic substance-containing liquid is treated by the membrane-separated activated sludge method, the state of the Flectobacillus strain group bacteria present in the mixed liquid of the soluble organic substance-containing liquid and the activated sludge is determined. Since the processing is performed while monitoring, the processing conditions can be controlled according to the result. For this reason, it is possible to predict and detect viscosity increase and clogging of the membrane at an early stage and take measures to prevent various phenomena that impair processing stability such as poor membrane permeability and foaming / scum. Can be prevented.

  Hereinafter, the present invention will be described in detail and specifically.

  In the present invention, a soluble organic substance-containing liquid such as waste water is treated by, for example, the treatment apparatus shown in FIG. 1, and the finally obtained clarified liquid is soluble organic substance to such an extent that it can be discharged into a river or the like as it is. Has been disassembled.

  The processing apparatus shown in FIG. 1 includes a biological reaction tank 1 containing sludge containing microorganisms, a raw solution pump 4 for supplying a raw solution to the biological reaction tank 1, and a membrane separation for solid-liquid separation of the biologically processed processing liquid. Monitors the state of the bacteria of the group of the Streptococcus strains in the mixed liquid of the device 2, the suction pump 3 for sucking the separated liquid during the solid-liquid separation, and the soluble organic substance-containing liquid and the activated sludge in the biological reaction tank 1 And monitoring means 7. The membrane separation device 2 is immersed in a treatment liquid in the biological reaction tank 1, and oxygen is supplied below the membrane separation device 2 to advance the aerobic treatment and to clean the membrane surface. An aeration device 5 connected to is provided. In addition, a sludge extraction pump 13 for extracting excess sludge as necessary is installed below the biological reaction tank 1.

  The biological reaction tank 1 contains sludge containing microorganisms, and these microorganisms act as organic matter-degrading bacteria and further decompose these microorganisms to perform biological treatment. Therefore, the bioreactor 1 has an inner surface with no corners so that sludge is not partially unevenly distributed and oxygen is uniformly supplied and partially anaerobic. The thing without an unevenness | corrugation is preferable. As a result, the temperature and pH of the treatment liquid become uniform in the biological reaction tank 1.

  Microorganisms contained in sludge contribute to the degradation of soluble organic matter such as bacteria, yeasts and fungi including fungi, and are obtained from nature, such as soil, compost, and sludge, by accumulating culture and acclimatization. The It is also possible to isolate and use the main microbial group involved in the degradation from this conditioned solution.

  The biological reaction tank 1 must contain other components necessary for the growth of microorganisms. Therefore, for example, nitrogen, phosphorus, potassium, sodium, magnesium and other metal salts are added to the bioreactor, except when already contained in the stock solution.

  As the membrane separation device 2 provided in the biological reaction tank 1, a module formed using a microfiltration membrane, an ultrafiltration membrane, a nanofiltration membrane, a reverse osmosis membrane, or the like can be used. From the economical point of view, a module using a microfiltration membrane or an ultrafiltration membrane that has a high filtration rate and can be made compact and is easy to maintain is preferable. The membrane may be a flat membrane, a hollow fiber membrane or the like. The form of the module is not particularly limited, but in this embodiment, an immersion type membrane module is used for space saving. In the case of the immersion type, the shape is preferably such that the fouling substance can be successfully removed by combination and arrangement with an aeration apparatus or a stirring apparatus. Furthermore, as a filtration method in the membrane separation device 2, there are a cross flow method and a total amount filtration method. If the cross flow method is employed, filtration can be performed while removing the membrane surface.

  In addition, as a monitoring means 7 for monitoring the state of the bacteria of the Streptococcus strain group in the mixed solution of the soluble organic substance-containing liquid and the activated sludge in the biological reaction tank 1, genetic information of the Streptococcus strain group bacteria, RNA transcription characteristics Based on protein translation characteristics, expressed protein-generating substance characteristics, morphological characteristics, and the like, it is possible to use those that monitor the status of the bacteria of the family Flectobacillus.

  Specifically, when monitoring based on genetic information, a fluorescence measuring device such as a fluorescence microscope or a fluorescence image scanner, a nucleic acid hybridization detection device, a flow cytometer, a thermal cycler, and an electrophoresis device are used. In addition, when monitoring based on gene expression characteristics such as RNA transcription characteristics, protein translation characteristics, expressed protein product characteristics, etc., fluorescence microscope, flow cytometer, electrophoresis apparatus, mass spectrometer, image analyzer, spectrophotometer When monitoring based on morphological features, such as a meter, a fluorescence measuring device, a luminescence measuring device, etc., a microscope, a microscope-attached image recording means and an image analyzing means thereof may be used.

  The monitoring means 7 is provided with a processing condition control device 8 that controls processing conditions based on the monitoring result. For example, the processing condition control device 8 issues a processing condition adjustment signal 9 based on the monitoring result to the suction pump 3 to adjust the amount of membrane filtration water and suction pressure per hour, increase or decrease the membrane filtration operation time, and the like. In addition, the processing condition adjustment signal 10 for controlling the atmosphere of the biological reaction tank 1 can be issued to adjust the pH, temperature, aeration amount, and stirring conditions. The details of the soluble organic substance-containing liquid flowing into the biological reaction tank 1 Processing condition adjustment signal 11 to control the conditions, adjustment of sludge load amount, adjustment of types of pollutants contained in soluble organic substance-containing liquid, addition of antibiotics and other chemicals and specific viruses, adjustment of addition amount of trace elements, etc. It can also be done. And in order to control the sludge residence time in the biological reaction tank 1, the process condition adjustment signal 12 can be emitted and the sludge extraction amount can also be adjusted. Furthermore, the water residence time can be adjusted through these adjustments.

  In the processing apparatus configured as described above, the soluble organic substance-containing liquid is processed as follows. The soluble organic substance-containing liquid is supplied to the biological reaction tank 1 by the stock solution pump 4, and the soluble organic substance is decomposed by the activated sludge in the biological reaction tank 1. At this time, the biological reaction tank 1 is kept aerobic by being supplied with oxygen by the aeration apparatus 5 connected to the blower 6. And the liquid mixture of a soluble organic substance containing liquid and activated sludge is provided to the membrane separator 2, and is isolate | separated into activated sludge and a clear processing liquid.

  And in this invention, about the liquid mixture of the soluble organic substance containing liquid and activated sludge which are used for membrane separation, the monitoring means 7 monitors the state of the Fructobacillus strain group bacteria. It is preferable to adjust the processing conditions based on the monitoring result.

  Examples of adjustment of treatment conditions include adjustment of sludge load in biological reaction tank 1, type adjustment of pollutants contained in soluble organic substance-containing liquid, pH adjustment, temperature adjustment, aeration amount adjustment, agitation condition adjustment, antibiotics, etc. Addition of chemicals, adjustment of trace elements such as nitrogen, phosphorus and magnesium, adjustment of sludge residence time / water residence time, addition of specific viruses, adjustment of membrane filtration water volume and suction pressure per hour, increase / decrease of membrane filtration operation time, Adjustment of the adjustment tank that adjusts the inflow load fluctuation, etc. can be mentioned, and through these methods, control is performed so that the monitoring situation before the trouble occurs is closer. In addition, the type adjustment of the pollutant is, for example, when sugar wastewater and lipid wastewater discharged in separate processes are mixed and biologically treated, the input of sugar wastewater is temporarily suspended or the ratio is reduced, and the lipid wastewater is reduced. It is an adjustment that increases the input ratio temporarily.

  Fructobacillus group bacteria can be monitored by growing them by a plate culture method using a normal agar medium or Flectobacillus group bacteria selection medium. For example, as described below, the genetic information of the Flectobacillus group bacteria It is highly accurate and efficient to observe changes in number, concentration, dominance, morphology, and characteristics over time based on RNA transcription characteristics, protein translation characteristics, product characteristics, morphological characteristics, etc. This is preferable.

<Monitoring method 1: Genetic information / monitoring based on specific gene sequence>
Methods for monitoring the number, concentration, and dominance of the bacteria in the strain of the Streptococcus group based on genetic information include hybridization homology values for the entire chromosomal genome, and gene sequences specific to the bacteria in the group of Streptobacillus strains. Although there is a method based on the above, it is preferable to perform monitoring based on the latter method from the viewpoint that it can be easily and effectively confirmed. Specific gene sequence targets include 16S ribosomal RNA gene, 23S ribosomal RNA gene, gyrB gene sequence, spacer region between ribosomal RNA genes, and the like. Further, the monitoring target may be all or a part of the bacteria of the Streptococcus group, and may further include other bacteria as long as it can monitor the bacteria of the Streptococcus group. .

  When monitoring the dominance of the bacteria of the Fructobacillus family, for example, measurements that reflect as much as possible the total number of prokaryotes, total bacteria, and bacteria of a certain taxonomic group are performed at the same time, and the ratio of these is superior. It is good to calculate fortune-telling.

  As a method for monitoring a specific gene sequence, a known method can be used. For example, a region specific to a Fructobacillus strain bacterium is selected from gene sequence information, and an oligonucleotide having the gene sequence or A method using the hybridization efficiency of the polynucleotide can be used. Specifically, a dot hybridization method, a microarray method, an in situ hybridization method, or the like can be used using a fluorescent substance, a radioisotope, an enzymatic reporter molecule, an intercalator having electric activity, or the like. As the in situ hybridization method, a fluorescence in situ hybridization method (FISH method) using a fluorescently labeled oligonucleotide or a fluorescently labeled polynucleotide can be used. In addition, what is necessary is just to implement FISH method, enhancing a signal as needed. As a detection method by the FISH method, a method using a fluorescence microscope or a method using a flow cytometer may be used. In the dot hybridization method and microarray method, oligonucleotides or polynucleotides having gene sequences specific to the bacteria of the Streptococcus group are immobilized on a membrane filter or substrate, and the nucleic acid derived from the sludge to be detected is fluorescent. And the like, which are carried out by hybridization of those labeled with the above.

  As a method of utilizing the hybridization efficiency of an oligonucleotide or polynucleotide having a 16S ribosomal RNA gene sequence specific to the Fructobacillus strain group bacteria, the base sequence described in SEQ ID NO: 1 or a part of the base sequence corresponding thereto is used. And a DNA or RNA probe that can specifically hybridize with the nucleic acid of the bacteria belonging to the family Flectobacillus strain. For example, DNA probe “R-FL615” (Simek K, Pernthaler J, Weinbauer MG, Hornak K, Dolan JR, Nedoma J, Masin M, Amann R. Appl Environ Microbiol. 2001 Jun; 67 (6): 2723-33. And a probe having the base sequence set forth in SEQ ID NOs: 2 to 35 or a base sequence corresponding thereto. Of course, in addition to these probes, any probe designed to be able to detect the Fructobacillus strain bacteria by hybridization can be used.

  In addition, as a method for monitoring a specific gene sequence, there is also a method for detecting and quantifying by applying a polymerase chain reaction method (PCR) using a primer DNA having a gene sequence specific to the bacteria of the Streptococcus group. . Examples of such a method include a quantitative PCR method in which nucleic acid amplification is detected in real time using a primer set specific to the bacteria of the group of Streptobacillus strains. Furthermore, the genomic DNA of microorganisms contained in the sludge mixture is extracted, PCR amplification is performed using a primer set specific to prokaryotes in general, bacteria in general, or a narrower taxonomic group, and the Flectobacillus strain is obtained by electrophoresis. It is also possible to discriminate and monitor the gene sequences of group bacteria. As this electrophoresis method, denaturant concentration gradient gel electrophoresis (DGGE) can be used.

<Monitoring Method 2 / Monitoring Morphological Features>
Causes of troubles in the membrane separation activated sludge method, such as membrane permeability failure due to clogging of membranes, generation of severe foaming and scum, deterioration of aeration efficiency due to increased viscosity, sludge fluidity reduction, entrapment of bubbles, Fractobacillus bacteria play an important role, but it is thought that the secretion of viscous substances accompanying changes in morphological characteristics as well as abundance and dominance is an important factor. Therefore, by monitoring and correlating the morphological change of the Fructobacillus bacteria related to the secretion of the viscous substance and the occurrence trouble, it becomes possible to predict and detect the trouble more accurately and take measures at an early stage. Specifically, when trouble occurs, the form of the bacteria of the Flectobacillus strain is changing to a spiral, long fungus or fibrous form (that is, a form extending in the long axis direction). Measures when the form starts to change to a spiral, long fungus, or fibrous form through monitoring. As a method for monitoring morphological features, monitoring with a microscope or automatic monitoring with a microscope-attached image recording means and its image analysis means can be performed. At that time, a fluorescence in situ hybridization method (FISH method) using a fluorescently labeled oligonucleotide or a fluorescently labeled polynucleotide targeting Flectobacillus strain bacteria may be used.

<Monitoring method 3 / gene expression characteristics>
Furthermore, the gene expression characteristic can also be used as a method for monitoring the bacteria of the family Flectobacillus. The gene expression characteristics refer to RNA transcription characteristics, protein translation characteristics, and production substance characteristics (for example, expressed protein production substance characteristics) from the bacteria genome of the Streptococcus strain. The properties of the Streptococcus strain bacteria vary greatly depending on the growth conditions. Therefore, tracking property changes with gene expression characteristics is very useful in the operational stability of the membrane separation activated sludge process, and it is more accurate at an early stage by monitoring gene expression characteristics and occurrence troubles in association with each other. It is possible to predict and detect troubles and take countermeasures. The target of gene expression characteristics is not particularly limited, and may be any constituent gene or regulatory gene.

  The target of RNA transcription characteristics may be either messenger RNA or ribosomal RNA. Various known methods can be used as a method for monitoring these RNA transcription characteristics, such as a method for analyzing the abundance of messenger RNA by hybridization, a method using a DNA chip using an image analyzer, and the like. Is mentioned. Also, the FISH method can be used.

  In addition, the target protein of the protein translation characteristic is not particularly limited, and examples thereof include a morphological change-inducing protein and an extracellular polymer-forming protein. As a method for monitoring protein translation characteristics, various known protein analysis methods such as two-dimensional electrophoresis, Western blot, and mass spectrometer can be used.

  Furthermore, it is not particularly limited as a target of the product substance characteristics, and examples thereof include extracellularly generated polymers and intracellular components. Examples of methods for monitoring extracellularly produced polymers and intracellular components include methods using a liquid chromatography device, a gas chromatography device, a mass spectrometer, a spectrophotometer, a fluorescence measuring device, a luminescence measuring device, etc. An ELISA method using an antibody-antigen reaction can also be applied.

<Example 1>
(1) Prepare two series of membrane separators, which are modularized PVDF microfiltration flat membranes with a pore size of 0.1 μm, immersed in a bioreactor with an effective volume of 30 L, and dextrin artificial wastewater with 1000 ppm BOD Treated by the separated activated sludge method. The residence time of the artificial wastewater in the biological reaction tank was 1 day, and the treatment was performed until the membrane permeation performance, the quality of the permeated water, and the monitoring state of the Fructobacillus strain group bacteria by the PCR-DGGE method were stabilized.

  Monitoring by PCR-DGGE method was performed as follows. Extraction and purification of genomic DNA using the Ultra Clean Soil DNA Isolation Kit (manufactured by MO BIO laboratories) using about 0.5 ml of the mixture in the biological reaction tank extracted to 0.2 ml by centrifugation. Next, according to the method reported by Ishii et al. (Ishii et al., Microbes and Environments 15, 2000, P59-73), a part of 16S ribosomal RNA gene was PCR amplified and denaturing gradient gel electrophoresis was performed. . Then, by monitoring the brightness of the band showing the same mobility as that of the strain Flectobacillus major DSMZ 103 isolated from the mixed solution and the strain Flectobacillus major DSMZ 103, the superiority of the strain Flectobacillus strain was confirmed. In addition, when the base sequence of the monitored band was decoded and homology search was performed using an international official base sequence database, it was confirmed that the bacteria were Flectobacillus strains.

  In addition, when the treatment was stable, the Streptococcus strain bacteria contained in the mixed solution in the biological reaction tank were also monitored by the FISH method and the dot hybridization method. However, the Flectobacillus strain bacteria were not detected by both methods.

  In the FISH method, DNA probe “R-FL615” (Simek K, Pernthaler J, Weinbauer MG, Hornak K, Dolan JR, Nedoma J, Masin M, Amann R. Appl Environ Microbiol. 2001 Jun; 67 (6): 2723-33.) And the probe shown in SEQ ID NO: 16 were used to detect the Fructobacillus strain bacteria contained in the 4% paraformaldehyde-fixed sample of the biological reaction tank mixture. Hybridization conditions were performed at 46 ° C. in the presence of a buffer containing 35% formamide.

In the dot hybridization method, “R-FL615” and the probe shown in SEQ ID NO: 2 are immobilized on a membrane filter, and the amount of hybridization of RNA derived from the mixed liquid sample in the biological reaction vessel labeled with rhodamine is measured by fluorescence. did. Hybridization conditions were performed according to the FISH method.
(2) After that, biological treatment is carried out with the BOD concentration of dextrin-based artificial wastewater supplied to the biological reaction tank being alternately set to 750 ppm or 1250 ppm every 6 hours, and 7 days after the change in BOD concentration. Was monitored. As a result, the sludge concentration and viscosity of the mixed liquid in the biological treatment tank were constant before and after the change, which were 10 g / L and 25 mPa · s, respectively. However, depending on the PCR-DGGE method, The increase in bacteria could be confirmed, and the presence was also detected by FISH method and membrane hybridization method.
(3) Therefore, the BOD concentration of dextrin-based artificial wastewater flowing into the biological reaction tank is returned to 1000 ppm again for one of the two lines (series A), and the other series flows into the biological reaction tank. Biological treatment was carried out by changing the BOD concentration of dextrin-based artificial wastewater as it was every 6 hours to 750 ppm or 1250 ppm alternately (series B). As a result, the viscosity of the mixed liquid of series B increased to 100 mPa · s after 3 days and the differential pressure between the stock side and the filtrate side of the PVDF membrane increased, whereas the viscosity of series A was 25 mPa · s. The differential pressure did not increase.

Then, when the mixed solution in both series of bioreactors was monitored by the PCR-DGGE method, the Bacterial strain Bacteria were observed before the BOD concentration fluctuation in (2) above was performed in Series A. While it decreased to the level of, it increased further in Series B. In addition, when Fectobacillus strains were monitored by the FISH method, no Fractobacillus strains were detected in line A.
(4) In this way, by monitoring the bacteria of the Streptococcus strain, it is possible to predict the deterioration of membrane permeability seen in the increase in differential pressure earlier than the monitoring of viscosity. It was possible to prevent poor water permeability.

<Comparative Example 1>
In each of the steps (1) to (3) of Example 1, Eikelboom Type 021N (Eikelboom Type 021N) contained in a 4% paraformaldehyde-fixed sample of the mixed solution in the reaction tank, not in the Flectobacillus strain group Bacteria were detected. The detection was performed by the FISH method using a DNA probe “AP3” (Patent Document 2). Hybridization conditions were performed at 46 ° C. in the presence of a buffer containing 35% formamide. As a result, we tried to detect one AP3 strain of Eikelboom 021N using a fluorescence microscope, but Eikelboom Type 021N bacteria were not detected in any biological reaction tank in any process, and Eikelboom Type 021N bacteria were detected. It was found that an increase in viscosity and poor membrane permeability cannot be predicted.

It is a schematic flowchart of the processing apparatus which shows one Embodiment of this invention.

Explanation of symbols

1: Bioreaction tank 2: Membrane separation device 3: Suction pump 4: Stock solution pump 5: Aeration device 6: Blower 7: Monitoring means 8: Treatment condition control device 9-12: Treatment condition adjustment signal 13: Sludge extraction pump

Claims (8)

  When treating a soluble organic substance-containing liquid by the membrane-separated activated sludge method, the treatment is performed while monitoring the state of the Flectobacillus family bacteria in the mixed liquid of the soluble organic substance-containing liquid and the activated sludge. A method for treating a soluble organic substance-containing liquid.   When processing soluble organic matter-containing liquids by the membrane-separated activated sludge method, control the processing conditions by monitoring the state of the Fructobacillus family bacteria present in the mixture of soluble organic-containing liquids and activated sludge. A method for treating a soluble organic substance-containing liquid characterized by the above.   The method for treating a solution containing a soluble organic substance according to claim 1 or 2, wherein the state of the bacteria of the group Fructobacillus is monitored based on genetic information of the bacteria of the group Fectobacillus.   The soluble organic matter according to any one of claims 1 to 3, wherein the state of the bacteria of the Streptococcus group is monitored based on at least one of RNA transcription characteristics, protein translation characteristics, and product characteristics of the bacteria. Processing method of contained liquid.   The processing method of the soluble organic substance containing liquid in any one of Claims 1-4 which monitors the state of a Fructobacillus strain group bacteria based on the morphological characteristic of a Flectobacillus strain group bacteria.   In a biological reaction tank for treating a soluble organic substance-containing liquid with activated sludge, a separation membrane for solid-liquid separation of a mixed liquid of the soluble organic substance-containing liquid and activated sludge, and a mixed liquid of the soluble organic substance-containing liquid and activated sludge A device for treating a solution containing a soluble organic matter, comprising: a monitoring means for monitoring the state of the bacteria of the genus Flectobacillus.   The monitoring means monitors the state of the bacteria of the Streptococcus group based on at least one of genetic information, RNA transcription characteristics, protein translation characteristics, product characteristics and morphological characteristics of the bacteria. The processing apparatus of the soluble organic substance containing liquid of Claim 6.   8. The processing apparatus for a soluble organic substance-containing liquid according to claim 6 or 7, wherein the monitoring means includes processing condition control means.

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