JP4328664B2 - 化学バイオセンサおよびその製造方法 - Google Patents
化学バイオセンサおよびその製造方法 Download PDFInfo
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- JP4328664B2 JP4328664B2 JP2004127340A JP2004127340A JP4328664B2 JP 4328664 B2 JP4328664 B2 JP 4328664B2 JP 2004127340 A JP2004127340 A JP 2004127340A JP 2004127340 A JP2004127340 A JP 2004127340A JP 4328664 B2 JP4328664 B2 JP 4328664B2
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- thin film
- hydroxyapatite
- chemical
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- 239000000126 substance Substances 0.000 title claims description 39
- 238000004519 manufacturing process Methods 0.000 title claims description 8
- 239000010409 thin film Substances 0.000 claims description 60
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 51
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 40
- 108090000623 proteins and genes Proteins 0.000 claims description 24
- 102000004169 proteins and genes Human genes 0.000 claims description 24
- 239000000758 substrate Substances 0.000 claims description 18
- 239000011575 calcium Substances 0.000 claims description 14
- 238000000608 laser ablation Methods 0.000 claims description 10
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 4
- 229910052791 calcium Inorganic materials 0.000 claims description 4
- 239000007789 gas Substances 0.000 description 20
- 238000002441 X-ray diffraction Methods 0.000 description 16
- 239000010408 film Substances 0.000 description 12
- 239000011734 sodium Substances 0.000 description 8
- 238000001179 sorption measurement Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 239000004065 semiconductor Substances 0.000 description 6
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 5
- 239000013078 crystal Substances 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 4
- 239000011777 magnesium Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000010897 surface acoustic wave method Methods 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000000919 ceramic Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000012091 fetal bovine serum Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000010453 quartz Substances 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 238000004544 sputter deposition Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 229910000756 V alloy Inorganic materials 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000001771 vacuum deposition Methods 0.000 description 2
- 238000007740 vapor deposition Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229910018072 Al 2 O 3 Inorganic materials 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 229910014497 Ca10(PO4)6(OH)2 Inorganic materials 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- GEIAQOFPUVMAGM-UHFFFAOYSA-N Oxozirconium Chemical compound [Zr]=O GEIAQOFPUVMAGM-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 229920001940 conductive polymer Polymers 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229910052712 strontium Inorganic materials 0.000 description 1
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
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- Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
Description
K.Persaud and G.Dodd:Nature,299,352−354(1982).. K.C.Persaud and P.Travers:Intelligent Insturuments & Computers,Elsevier Science Publishing,147−154(1991). I.Lundstrom,R.Erlandssom and U.Frykman:Nature,352,47−50(1991). A.Saitou,T.Nomura and T.Moriizumi:Mol.Cryst.Liq.Cryst.,267,387−392(1995). K.Ema,M.Yokoyama,T.Nakamoto and T.Moriizumi:The 17th SensorSymposium,307−312(200).
この発明に用いるキャリアドーピング用元素Mとしては、例えば、ナトリウム(Na)、カリウム(K)、マグネシウム(Mg)、ストロンチウム(Sr)などが挙げられる。ハイドロキシアパタイトの基本物性を損なわない範囲であれば、カルシウムの1.0wt%に対して、置換されるキャリアドーピング用元素の重量%は0〜0.1wt%である。
本発明に適用するレーザーアブレーション法の薄膜形成操作は慣用法でよい。レーザーアブレーション法に用いるレーザーの種類は特に限定されないが、膜厚が1μm程度あるいは1μm以下の薄膜を得るためには例えばエキシマレーザを用いることが望ましい。
図1において、この実施形態の化学バイオセンサは、アルミナ製の基板1と、この基板1上に成膜されたハイドロキシアパタイトまたはハイドロキシアパタイト誘導体の薄膜2と、薄膜2上に離間して形成された櫛型で一対の電極3,3と、電極3,3にそれぞれ結線されて外部の電気特性測定装置(LCRメータなど、図示省略)に接続されるリード線4,4とから構成されている。この実施形態に係る化学バイオセンサは次の実施例1によって更に詳しく説明される。
尚、上記のように形性した薄膜2はX線回折分析に供した。測定した薄膜2のX線回折パターンを図5に示す。図5のX線回折パターンから明らかなように、ポーラスアルミナ上の薄膜2は、目的とする結晶化ナトリウム置換ハイドロキシアパタイトであった。
また、上記ではレーザーアブレーション法を用いて薄膜を形成したが、本発明においてはレーザーアブレーション法の替わりに、スパッタリング法、反応性蒸着法などを用いて成膜しても構わない。
2 薄膜
3 電極
4 リード線
Claims (3)
- 基板上にハイドロキシアパタイトの薄膜を形成し、該薄膜上に更に、電気抵抗測定用の一対の電極を離間して設けて成り、前記一対の電極間の電気抵抗によりタンパク質を検出する化学バイオセンサ。
- 基板上に、ハイドロキシアパタイトを構成するカルシウムの一部をキャリアドーピング用元素で置き換えてなるハイドロキシアパタイト誘導体の薄膜を形成し、該薄膜上に更に、電気抵抗測定用の一対の電極を離間して設けて成り、前記一対の電極間の電気抵抗によりタンパク質を検出する化学バイオセンサ。
- 請求項1または請求項2に記載の化学バイオセンサを製造するにあたり、ハイドロキシアパタイトの薄膜またはハイドロキシアパタイト誘導体の薄膜を基板上に形成する薄膜形成工程を含む製造方法であって、前記薄膜形成工程においてレーザーアブレーション法によりハイドロキシアパタイトの薄膜またはハイドロキシアパタイト誘導体の薄膜を形成することを特徴とする化学バイオセンサの製造方法。
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JP4328664B2 true JP4328664B2 (ja) | 2009-09-09 |
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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US8052932B2 (en) | 2006-12-22 | 2011-11-08 | Research Triangle Institute | Polymer nanofiber-based electronic nose |
WO2006025358A1 (ja) * | 2004-08-31 | 2006-03-09 | Japan Science And Technology Agency | 化学センサ装置用の検出子およびその利用 |
JP4900690B2 (ja) * | 2005-11-25 | 2012-03-21 | 独立行政法人物質・材料研究機構 | バイオセンサー構成体とその製造方法 |
JP6588765B2 (ja) * | 2015-08-07 | 2019-10-09 | 学校法人 神野学園 | Dna吸着担体及びその利用方法 |
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