JP4296169B2 - Small liquid inhalation droplet method - Google Patents

Description

The present invention relates to a method for inhaling droplets of trace liquid.

2. Description of the Related Art Conventionally, there is a dispensing device (a trace liquid inhalation flying device) for dispensing a liquid such as a reagent product, an aqueous solution, a solvent, protein, or DNA into a container (plate).
As a conventional dispensing device, the inside of a pipe-shaped line is brought into a negative pressure state to suck in liquid, and after the inside of the line is brought to a desired positive pressure state, an on-off valve is opened momentarily to remove a small amount of liquid. There are those that fly (see, for example, Patent Document 1).
JP-A-9-145720

However, conventionally, when inhaled liquid again after Hishizuku, it opened the closing valve immersed tip into the liquid of the nozzle attached to the line (pipette tip), (after Hishizuku) in line positive pressure Because of the state, the expansion of the air compressed in the line may cause bubbles 40 to be generated in the leaked liquid from the tip of the nozzle as shown in FIG.
If air bubbles are generated at the tip of the nozzle in this way, the air bubbles may obstruct the liquid from being sucked. If air bubbles are also inhaled when inhaling the liquid, the correct amount (predetermined amount) of the liquid may not be inhaled or the inhaled liquid may not be ejected vigorously. There was a problem that the amount of droplets after that became inaccurate.
Depending on the type of liquid to be inhaled, it is not preferable that bubbles are generated in the liquid.
SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a method for injecting and dropping a trace amount of liquid that does not cause air to leak from the tip of a nozzle immersed in the liquid when the liquid is inhaled.

In order to achieve the above object, a method for inhaling droplets of a small amount of liquid according to the present invention includes a shared flow channel, a first branched flow channel and a second branched flow branched and arranged upstream of the shared flow channel. A positive pressure generating means disposed upstream of the first branch flow path, a negative pressure generating means disposed upstream of the second branch flow path, and a downstream side of the common flow path and connected to the opening and closing valve, at the closed state of the electric control unit and, the extremely thin tube for sucking-Hishizuku the liquid while being attached to the on-off valve, the upper SL-off valve for controlling the opening and closing of the closing valve once a switching means for switching and in the atmospheric pressure to the negative pressure state after holding a large-pressure state, inhalation flying droplet device having a positive pressure state switches the in-off valve from the negative pressure state to a positive pressure Te A method for injecting and ejecting a small amount of liquid using a liquid, and after the liquid is ejected from the microtubule, the on-off valve is closed to open the liquid. The inside of the valve is set to a positive pressure state, the switching means is switched to bring the inside of the on-off valve to an atmospheric pressure state, the tip of the micro tube is immersed in a liquid while the inside of the on-off valve is kept at an atmospheric pressure state, and the on-off valve Is opened and then the switching means is switched to place the open / close valve in a negative pressure state and suck the liquid into the microtubule.

In addition, a first switching valve that switches the positive pressure generating means and the atmosphere opening to communicate with the first branch flow path, the first branch flow path, and the switch to the switching means of the suction flying device. A second switching valve that switches between the second branch flow path and communicates with the shared flow path, and connects the positive pressure generating means and the first branch flow path by the first switching valve, and After the first branch flow path and the shared flow path are communicated with each other by the second switching valve to drop liquid from the microtubule, the open / close valve is closed, and the open / close valve is brought into a positive pressure state. The first switching valve communicates with the first branch flow path and the atmosphere opening to bring it into an atmospheric pressure state, and the tip of the microtubule is immersed in a liquid while the inside of the on-off valve is kept in an atmospheric pressure state. Open the on-off valve and then turn off the second switching valve Instead it is a method for sucking liquid into the ultrafine tube to a negative pressure in the interior of the switch valve communicates between the shared channel and the second branch flow channel.

Further, the switching means of the suction flight drop device, a first switching valve for connecting communicating with the common flow path by switching between the first branch passage and the second branch channel, and the negative pressure generating means A second switching valve that switches to the atmosphere opening and communicates with the second branch flow path. The first switching valve allows the positive pressure generating means and the shared flow path to communicate with each other. After the second branch flow channel and the atmosphere opening are communicated by a switching valve to drop liquid from the microtubule, the on-off valve is closed to bring the inside of the on-off valve into a positive pressure state, and then Switching the first switching valve to connect the shared flow path and the second branch flow path to bring the inside of the on-off valve to an atmospheric pressure state, and keeping the inside of the on-off valve at an atmospheric pressure state, the tip of the ultrathin tube In the liquid, open the on-off valve, and then By switching the changeover valve is a method for sucking liquid into the ultrafine tube with the negative pressure generating means and the second branch flow channel and communicated not in the opening and closing valve in a negative pressure state.

The present invention has the following remarkable effects.
According to the trace liquid inhalation droplet method according to the present invention, before the liquid is inhaled, it is possible to release the positive pressure state in the on-off valve with the on-off valve closed and to temporarily return to the atmospheric pressure state. When inhaling the liquid, it is possible to prevent air from leaking into the liquid from the tip of the microtubule immersed in the liquid.
In other words, before the liquid is inhaled, the compression of the air in the on-off valve can be released. Therefore, as in the past, the on-off valve is opened in a positive pressure state, and the tip of the microtubule is expanded by the expansion of the compressed air inside. Air is not pushed out into the liquid from the air and bubbles are not generated. Therefore, when inhaling the liquid, the problem that the bubbles are obstructed and it is difficult to inhale the liquid or the bubbles are also inhaled together is solved, and the precise amount of liquid is inhaled and vigorously ejected. Can do.

Hereinafter, the present invention will be described in detail with reference to the drawings illustrating embodiments.
1 to 3, the apparatus used in the method of the present invention includes a shared flow path 10, and the first branch flow path 11 and the first flow path are disposed upstream of the shared flow path 10. A bifurcated flow path 12 is branched. Positive pressure generating means 1 is provided at the upstream end of the first branch flow path 11, and negative pressure generating means 2 is provided at the upstream end of the second branch flow path 12.
Furthermore, this apparatus is attached to the on-off valve 3 connected to the downstream end of the common flow path 10, the electrical control unit 4 for controlling the on-off of the on-off valve 3, and attached to the on-off valve 3, while supplying liquid. And an ultrathin tube 5 for inhaling and flying droplets.
When the on-off valve 3 is in the closed state, there is provided switching means 6 for switching the inside of the on-off valve 3 from the negative pressure state to the positive pressure state and switching from the positive pressure state to the atmospheric pressure state temporarily to the negative pressure state. Yes.

  The positive pressure state and the negative pressure state are a state in which a pressure higher than the atmospheric pressure is acting on the basis of the atmospheric pressure (gauge pressure), and a state in which a pressure lower than the atmospheric pressure is acting. It is defined as a negative pressure state.

  Specifically, the positive pressure generating means 1 is a compressor 8, the negative pressure generating means 2 is a vacuum pump 9, and the common flow path 10, the first branch flow path 11, and the second branch flow path 12 are flexible, for example. It consists of a small diameter hose that has the property.

The switching means 6 includes a solenoid-operated first switching valve 21 and a second switching valve 22, and the first switching valve 21 has an atmosphere opening 7 that opens to the atmosphere side (outside).
The compressor 8 (positive pressure generating means 1) is connected to the upstream end of the first branch flow path 11 via the first switching valve 21. That is, the compressor 8 (positive pressure generating means 1) and the atmosphere opening 7 can be switched by the first switching valve 21 so as to communicate with the first branch flow path 11.

Further, the downstream end portions of the first branch flow channel 11 and the second branch flow channel 12 are connected to the upstream end portion of the common flow channel 10 via the second switching valve 22. That is, the first switching flow path 11 and the second branch flow path 12 are switched by the second switching valve 22 so as to be connected to the common flow path 10.
1 shows the case where the on-off valve 3 is in a positive pressure state, FIG. 2 shows the case of an atmospheric pressure state, and FIG. 3 shows the case of a negative pressure state.

  As shown in FIG. 4, the on-off valve 3 is disposed around a block-shaped main body portion 13, a valve portion (plunger) 14 disposed in the internal space S of the main body portion 13, and the valve portion 14. Thus, the coil 15 wound around the main body 13 and the ultrathin tube 5 connected to the internal space S projectingly at the tip of the main body 13 are provided.

  The internal space S of the main body 13 connects the proximal end side space 16 to which the shared flow path 10 is connected, the distal end side space 17 to which the microtubule 5 is connected, and the proximal end side space 16 and the distal end side space 17. And a cylindrical communication hole 18. Here, the opening of the main body 13 into which the shared flow path 10 is inserted is referred to as a supply port 19, and the opening of the main body 13 into which the ultrathin tube 5 is inserted is referred to as an exhaust port 20.

The valve portion 14 is configured to cover and open the proximal end opening of the microtubule 5 while being slidably fitted in the communication hole 18. Specifically, the valve portion 14 is constantly elastically biased by the compression spring 23 so as to cover the proximal end opening of the microtubule 5, and the coil 15 is energized to resist the compression spring 23. Then, the base end opening of the microtubule 5 is opened. In other words, the ON / OFF switching of the energization to the opening / closing valve 3 switches the opening / closing of the valve unit 14.
In addition, a contact rod 24 with which the base end of the valve portion 14 abuts is fixed in the communication hole 18 so that the valve portion 14 does not pull up more than necessary (to reduce the time loss of opening and closing of the on-off valve 3). It is closely fitted.

The valve portion 14 has a plurality of axial groove portions 14a formed on the outer peripheral surface of the portion closely fitted in the communication hole 18, and the groove portion 14a and the distal end space 17 are always in communication.
The contact rod 24 has a plurality of axial grooves 24a formed on the outer peripheral surface of the portion closely fitted in the communication hole 18, and the groove 24a and the proximal end space 16 are always in communication.

  That is, if the proximal end space 16 and the distal end space 17 are always in communication with each other via the groove 24a of the contact rod 24 and the groove 14a of the valve portion 14, the proximal end space 16 is in a negative pressure state. The distal end space 17 is also in a negative pressure state, and when the proximal end space 16 is in a positive pressure state, the distal end side space 17 is also in a positive pressure state.

  The electrical control unit 4 is electrically connected to the coil 15 and is configured to energize the coil 15 for a certain period of time to open the on-off valve 3. That is, the amount of liquid sucked from the microtubule 5 is determined by the length of time that the on-off valve 3 is kept open in the negative pressure state, while the on-off valve 3 is opened in the positive pressure state. The amount of liquid discharged from the microtubule 5 is determined by the length of time for holding the liquid.

  The electrical control unit 4 is electrically connected to a central control unit (not shown) together with the first switching valve 21 and the second switching valve 22 (shown in FIGS. 1 to 3). Is configured to independently switch the ON / OFF switching of the electrical control unit 4 and the first switching valve 21 and the second switching valve 22.

In addition, the liquid drop inhalation and drop apparatus used in the method of the present invention is used for liquid crystal injection, reagent dispensing, and dispensing of trace liquids such as aqueous solutions, solvents, alcohol solutions, solvents (organic solvents), inks, and oils. The amount of liquid that is used for (spraying) and sucked in the negative pressure state of the on-off valve 3 is (temporarily) retained only in the ultrathin tube 5 and is ejected from the microtubule 5 Is set to 10 nl (nanoliter) to 500 μl (microliter).

Next, another embodiment of the apparatus used in the method of the present invention will be described.
7 to 9, the first branch flow channel 11 and the second branch flow channel 12 are branched and arranged on the upstream side of the common flow channel 10. A compressor 8 is provided as the positive pressure generating means 1 at the upstream end of the passage 11, and a vacuum pump 9 is provided as the negative pressure generating means 2 at the upstream end of the second branch flow path 12. .
In addition, the on-off valve 3 is connected to the downstream side of the common flow path 10, and when the on-off valve 3 is closed, the inside of the on-off valve 3 is switched from the negative pressure state to the positive pressure state and from the positive pressure state to the atmospheric pressure state once. And switching means 6 for switching to a negative pressure state.

The switching means 6 switches between a downstream end of the first branch flow path 11 and a downstream end of the second branch flow path 12 so as to communicate with the upstream end of the common flow path 10. A switching valve 21 and a second switching valve 22 that has an atmosphere opening 7 and is connected to the upstream end of the second branch passage 12 by switching between the atmosphere opening 7 and the vacuum pump 9. is doing.
7 shows a case where the inside of the on-off valve 3 is in a positive pressure state, FIG. 8 shows a case where the pressure is atmospheric, and FIG. 9 shows a case where the pressure is negative.
7 to 9, the same reference numerals as those in FIGS. 1 to 3 have the same configurations as those in FIGS.

Further, in another embodiment of the present apparatus used in the method of the present invention shown in FIG. 10, the compressor 8 is provided as the positive pressure generating means 1, the compressor 8, the ejector 25, as the negative pressure generating means 2, And a negative pressure flow path 27 for sending air from the compressor 8 to the ejector 25.
Specifically, the negative pressure flow path 27 for sending the air from the compressor 8 to the ejector 25 side and the positive pressure flow path 26 for sending the air to the first branch flow path 11 side are provided. The downstream end of the first is connected to the upstream end of the first branch flow path 11 via the first switching valve 21 having the atmosphere opening 7. That is, the first switching valve 21 is configured to be connected to the first branch channel 11 by switching between the positive pressure channel 26 and the atmosphere opening 7.

The ejector 25 is disposed at the upstream end of the second branch flow path 12, and the downstream end of the first branch flow path 11 and the second branch flow path 12 is connected via the second switching valve 22. And connected to the upstream end of the common flow path 10. That is, the second switching valve 22 is configured to switch between the first branch flow path 11 and the second branch flow path 12 so as to communicate with the common flow path 10, and the second branch flow path 12 and the common flow path 10 are connected to each other. When communicating, the ejector 25 evacuates the common flow path 10 with air from the compressor 8 (not shown). In this case, since the on-off valve 3 can be set to a positive pressure and a negative pressure by a single compressor 8, the apparatus can be downsized.
In FIG. 10, the same reference numerals as those in FIGS. 1 to 3 are the same as those in FIGS.

A method of using the method of the present invention and a method (action) of using the microscopic liquid inhalation flying droplet apparatus will be described.
In the embodiment shown in FIGS. 1 to 3, first, the on-off valve 3 is in a closed state as shown in FIG. At this time, it is assumed that the on-off valve 3 is in an atmospheric pressure state.
Then, as shown in FIG. 5 (A), the tip of the microtubule 5 is immersed in a liquid (such as a reagent) contained in the liquid container 28, and the open / close valve 3 is opened to suck the air in the internal space S to be negative. When a pressure is applied (see FIG. 3) and a predetermined amount of liquid is sucked into the microtubule 5, the on-off valve 3 is closed as shown in FIG.

  Next, in the closed state of the on-off valve 3, air is sent into the internal space S of the on-off valve 3 to make a positive pressure state (see FIG. 1), and a preload is applied. Then, as shown in FIG. 5 (C), the tip of the microtubule 5 is directed to one of the many recesses 29 formed in the dispensing container 30, and then the opening / closing valve 3 is opened to open the recess 29. The liquid splashes vigorously inside. At this time, a desired amount of liquid can be ejected by opening the on-off valve 3 for a predetermined time in the electrical control unit 4 (see FIG. 4).

  After the liquid is ejected, the on-off valve 3 is closed as shown in FIG. At this time, the internal space S is in a positive pressure state, but the internal space S is once communicated with the atmosphere (outside) while the on-off valve 3 is closed (see FIG. 2).

Then, with the inside of the on-off valve 3 kept at atmospheric pressure, the tip of the ultrathin tube 5 is immersed again in the liquid in the liquid container 28, and as shown in FIG. (Refer to FIG. 3) The liquid is inhaled, and then is dispensed into the dispensing container 30 to perform the dispensing operation.
It should be noted that the entire amount of the inhaled liquid can be set to be ejected at once, or can be set to be ejected at a plurality of times.

Further, in the embodiment shown in FIG. 1 to FIG. 3, from the time when the liquid is ejected to the time when the liquid is inhaled and then ejected again (in FIG. 5 (C) → (D) → (E) (A ) → (B) → (C) process} ON / OFF control method (operation procedure) of the on-off valve 3, the first switching valve 21, and the second switching valve 22, and the inside of the on-off valve 3 at that time (internal space) The state of the pressure P _{0 in} S) will be described in detail with reference to the state explanatory diagram of FIG.
First, after the liquid is dropped, as shown in FIG. 1, the first switching valve 21 communicates the compressor 8 and the first branch flow path 11, and the second switching valve 22 is shared with the first branch flow path 11. The on-off valve 3 is in communication with the flow path 10 and is in a positive pressure state. At this time, the first switching valve 21 and the second switching valve 22 are turned off.

  Then, after the liquid drops from the microtubule 5 in the positive pressure state, as shown in FIG. 2, the first switching valve 21 is turned on to allow the first branch flow path 11 and the atmosphere opening 7 to communicate with each other. The valve 3 is released (released from the positive pressure state) to the atmospheric pressure state. At this time, the inside of the shared flow path 10 and the first branch flow path 11 communicating with the internal space S of the on-off valve 3 are also in the atmospheric pressure state.

After the on-off valve 3 is brought to atmospheric pressure, the tip of the ultrathin tube 5 is immersed in a liquid to open the on-off valve 3, and the second switching valve 22 is turned on as shown in FIG. The on-off valve 3 is brought into a negative pressure state by communicating with the branch flow path 12, and a predetermined amount of liquid is sucked to close the on-off valve 3 (suction process K in FIG. 6).
Note that the first switching valve 21 in the ON state may be returned to the OFF state between the time when the second switching valve 22 is turned ON (in the negative pressure state) and the next time the liquid is ejected. .

  When the on-off valve 3 is closed and the liquid has been sucked in (the first switching valve 21 has already been turned off), the second switching valve 22 is turned off, and the on-off valve 3 is in the positive pressure state shown in FIG. To. Then, the on-off valve 3 is opened momentarily to drop the liquid into the dispensing container 30 (spraying step H in FIG. 6).

In the embodiment shown in FIG. 7 to FIG. 9, ON / OFF of the on-off valve 3, the first switching valve 21, and the second switching valve 22 from when the liquid is dropped to when the liquid is sucked and then dropped again. The control method (operation procedure) and the pressure state in the on-off valve 3 (internal space S) at that time will be described.
First, after the liquid is dropped, as shown in FIG. 7, the first switching valve 21 and the second switching valve 22 are both in the OFF state (the on-off valve 3) is in the positive pressure state. That is, the first switching valve 21 communicates the compressor 8 and the common flow path 10, and the second switching valve 22 communicates the second branch flow path 12 and the atmosphere opening 7.

  Then, after the liquid drops from the microtubule 5 in the positive pressure state, as shown in FIG. 8, the first switching valve 21 is turned on, and the second branch channel 12 opened to the atmosphere side in the common channel 10 is opened. The open / close valve 3 is brought into an atmospheric pressure state (releasing the positive pressure state) through communication.

After the on-off valve 3 is brought to atmospheric pressure, the tip of the ultrathin tube 5 is immersed in a liquid to open the on-off valve 3, and the second switching valve 22 is turned on as shown in FIG. The on-off valve 3 is brought into a negative pressure state by communicating with the flow path 12, and a predetermined amount of liquid is sucked to close the on-off valve 3.
When the on-off valve 3 is closed and the liquid has been sucked in (the first switching valve 21 has already been turned off), the second switching valve 22 is turned off, and the on-off valve 3 is in the positive pressure state shown in FIG. To. Then, the on-off valve 3 is opened momentarily to drop the liquid into the dispensing container 30.

As described above, the method for inhaling and ejecting a trace amount of liquid according to the present invention includes the common flow channel 10, the first branch flow channel 11 and the second branch flow channel 12 that are arranged to be branched upstream of the common flow channel 10. A positive pressure generating means 1 disposed upstream of the first branch flow path 11, a negative pressure generating means 2 disposed upstream of the second branch flow path 12, and a downstream of the common flow path 10. An on-off valve 3 connected to the side, an electric control unit 4 for controlling the on-off of the on-off valve 3, and an ultra-thin tube 5 attached to the on-off valve 3 and sucking / dropping liquid. 3 has switching means 6 for switching the inside of the on-off valve 3 from the negative pressure state to the positive pressure state and switching from the positive pressure state to the atmospheric pressure state to the negative pressure state in the closed state 3 before the liquid is sucked. In addition, with the on-off valve 3 closed, the positive pressure state in the on-off valve 3 can be released to temporarily return to the atmospheric pressure state. When, the air from the tip of the extremely thin tube 5 immersed in the liquid can be prevented from leaking into the liquid.
That is, since the compression of the air in the on-off valve 3 can be released before the liquid is sucked, the on-off valve 3 is opened in a positive pressure state as in the prior art, and the microtubules are expanded by the expansion of the compressed air inside. Air is not pushed out from the tip of 5 into the liquid and bubbles are not generated. Therefore, when inhaling the liquid, the problem that the bubbles are obstructing and it is difficult to inhale the liquid or the bubbles are also inhaled together is solved, and the precise amount of liquid is inhaled and vigorously ejected. Can do.

  In addition, the switching means 6 includes a first switching valve 21 that switches between the positive pressure generating means 1 and the atmosphere opening 7 and is connected to the first branch flow path 11, and the first branch flow path 11 and the second branch flow path. And a second switching valve 22 that is connected to the common flow path 10 by switching between 12 and 12 so as to prevent air from leaking into the liquid from the tip of the microtubule 5 immersed in the liquid when inhaled. It is possible to draw a small amount of liquid with a simple structure with high accuracy.

  The switching means 6 includes a first switching valve 21 that switches between the first branch flow path 11 and the second branch flow path 12 to communicate with the common flow path 10, the negative pressure generating means 2, and the atmosphere opening 7. And a second switching valve 22 that communicates with the second branch flow path 12 to prevent air from leaking into the liquid from the tip of the microtubule 5 immersed in the liquid. It is possible to draw a small amount of liquid with a simple structure with high accuracy.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is an overall simplified configuration explanatory diagram of a positive pressure state showing an embodiment of the present invention. It is a whole simplified structure explanatory view of an atmospheric pressure state. It is whole simplified structure explanatory drawing of a negative pressure state. It is a principal part longitudinal cross-sectional view. It is principal part action explanatory drawing. It is a state explanatory view. It is whole simplified structure explanatory drawing of the positive pressure state which shows other embodiment. It is a whole simplified structure explanatory view of an atmospheric pressure state. It is whole simplified structure explanatory drawing of a negative pressure state. It is whole simplified structure explanatory drawing of the positive pressure state which shows another embodiment. It is a principal part longitudinal cross-sectional view which shows a prior art example.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Positive pressure generating means 2 Negative pressure generating means 3 On-off valve 4 Electrical control part 5 Ultrathin tube 6 Switching means 7 Atmospheric opening
10 Common flow path
11 First branch flow path
12 Second branch flow path
21 1st switching valve
22 Second switching valve

Claims (3)

A common flow path (10), a first branch flow path (11) and a second branch flow path (12) arranged to be branched upstream of the common flow path (10), and the first branch flow Positive pressure generating means (1) disposed upstream of the passage (11), negative pressure generating means (2) disposed upstream of the second branch flow path (12), and the common flow An on-off valve (3) connected to the downstream side of the passage (10), an electric control unit (4) for controlling the on-off of the on-off valve (3), and a liquid attached to the on-off valve (3). ultra fine tube for sucking-Hishizuku (5), once atmospheric pressure from the positive pressure state with closed Te at switch-off valve (3) in a negative pressure state to a positive pressure above SL-off valve (3) A switching means (6) for switching to a negative pressure state after maintaining the atmospheric pressure state in a state, and a method for inhaling and ejecting a trace amount of liquid using an inhalation droplet apparatus,
After the liquid is sprayed from the ultrafine tube (5), the on-off valve (3) is closed to bring the inside of the on-off valve (3) into a positive pressure state, and the switching means (6) is switched to switch the on-off valve (3 ) Is set to atmospheric pressure, the on-off valve (3) is kept at atmospheric pressure, the tip of the ultrafine tube (5) is immersed in liquid, and the on-off valve (3) is opened before the switching means. (6) is switched, and the on-off valve (3) is brought into a negative pressure state to suck liquid into the microtubule (5) . A first switching valve that switches the positive pressure generating means (1) and the atmosphere opening (7) to the switching means (6) of the inhalation / splashing device so as to communicate with the first branch flow path (11). provided (21), the first branch channel (11) and the second changeover valve in which the second branch channel (12) and switches the coupling communicating with the shared channel (10) (22), the ,
The positive pressure generating means (1) and the first branch channel (11) are communicated with each other by the first switching valve (21), and the first branch channel (11) by the second switching valve (22). And the shared flow path (10) are communicated to drop liquid from the microtubule (5), the on-off valve (3) is closed, and the inside of the on-off valve (3) is brought into a positive pressure state.
Thereafter, the first switching valve (21) communicates the first branch passage (11) with the atmosphere opening (7) to bring it into an atmospheric pressure state, and the inside of the on-off valve (3) is brought to an atmospheric pressure state. The tip of the ultrathin tube (5) is immersed in a liquid, and the on-off valve (3) is opened, and then the second switching valve (22) is switched to switch the shared flow path (10) and the second branch flow. 2. The method of injecting droplets into a micro liquid according to claim 1 , wherein the liquid is sucked into the microtubule (5) by communicating with the passage (12) so that the on-off valve (3) is in a negative pressure state . The first switching channel (6) of the suction flying device is switched between the first branch channel (11) and the second branch channel (12) to be connected to the common channel (10). a switching valve (21), said negative pressure generating means (2) and the atmospheric opening (7) the second changeover valve switches the coupling communicating with the second branch channel (12) (22), the provided
The first switching valve (21) communicates the positive pressure generating means (1) with the shared flow path (10), and the second switching valve (22) communicates the second branch flow path (12) with the second flow path. After the liquid is splashed from the microtubule (5) by communicating with the atmosphere opening (7), the on-off valve (3) is closed to bring the inside of the on-off valve (3) into a positive pressure state,
Thereafter, the first switching valve (22) is switched so that the shared flow path (10) and the second branch flow path (12) communicate with each other, the inside of the on-off valve (3) is brought into an atmospheric pressure state, and the open / close With the inside of the valve (3) kept at atmospheric pressure, the tip of the ultrathin tube (5) is immersed in liquid, the on-off valve (3) is opened, and then the second switching valve (22) is switched to switch the negative pressure. The trace amount according to claim 1, wherein the generating means (2) and the second branch flow path (12) are communicated to bring the on-off valve (3) into a negative pressure state and suck the liquid into the microtubule (5). Liquid inhalation droplet method.

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