JP4279802B2 - 水処理管理方法 - Google Patents
水処理管理方法 Download PDFInfo
- Publication number
- JP4279802B2 JP4279802B2 JP2005155509A JP2005155509A JP4279802B2 JP 4279802 B2 JP4279802 B2 JP 4279802B2 JP 2005155509 A JP2005155509 A JP 2005155509A JP 2005155509 A JP2005155509 A JP 2005155509A JP 4279802 B2 JP4279802 B2 JP 4279802B2
- Authority
- JP
- Japan
- Prior art keywords
- bacteria
- nitrite
- water treatment
- tank
- indicator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims description 91
- 238000007726 management method Methods 0.000 title claims description 32
- 241000894006 Bacteria Species 0.000 claims description 238
- 238000000034 method Methods 0.000 claims description 116
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 90
- 229910052757 nitrogen Inorganic materials 0.000 claims description 44
- 238000012545 processing Methods 0.000 claims description 41
- GQPLMRYTRLFLPF-UHFFFAOYSA-N Nitrous Oxide Chemical compound [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 claims description 36
- 239000002351 wastewater Substances 0.000 claims description 34
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims description 19
- 238000005259 measurement Methods 0.000 claims description 14
- 239000001272 nitrous oxide Substances 0.000 claims description 10
- 230000001590 oxidative effect Effects 0.000 claims description 10
- 238000003753 real-time PCR Methods 0.000 claims description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 115
- 239000012634 fragment Substances 0.000 description 63
- 230000002860 competitive effect Effects 0.000 description 54
- 239000010802 sludge Substances 0.000 description 44
- 241001453382 Nitrosomonadales Species 0.000 description 21
- 239000007788 liquid Substances 0.000 description 14
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 12
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 10
- 230000001580 bacterial effect Effects 0.000 description 9
- 239000000203 mixture Substances 0.000 description 8
- 238000001556 precipitation Methods 0.000 description 8
- 238000000926 separation method Methods 0.000 description 8
- 238000005273 aeration Methods 0.000 description 7
- 229910002651 NO3 Inorganic materials 0.000 description 6
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 6
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 6
- 238000004891 communication Methods 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 241000894007 species Species 0.000 description 6
- 239000000758 substrate Substances 0.000 description 5
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 4
- 241000589516 Pseudomonas Species 0.000 description 4
- 108010006785 Taq Polymerase Proteins 0.000 description 4
- 239000011543 agarose gel Substances 0.000 description 4
- 238000000246 agarose gel electrophoresis Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 4
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 4
- 229960005542 ethidium bromide Drugs 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 238000012886 linear function Methods 0.000 description 4
- 230000000717 retained effect Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 241001057811 Paracoccus <mealybug> Species 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 229910001873 dinitrogen Inorganic materials 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- 108020000946 Bacterial DNA Proteins 0.000 description 2
- 101000666730 Homo sapiens T-complex protein 1 subunit alpha Proteins 0.000 description 2
- CGNLCCVKSWNSDG-UHFFFAOYSA-N SYBR Green I Chemical compound CN(C)CCCN(CCC)C1=CC(C=C2N(C3=CC=CC=C3S2)C)=C2C=CC=CC2=[N+]1C1=CC=CC=C1 CGNLCCVKSWNSDG-UHFFFAOYSA-N 0.000 description 2
- 102100038410 T-complex protein 1 subunit alpha Human genes 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000012295 chemical reaction liquid Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 125000004433 nitrogen atom Chemical group N* 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 241000589151 Azotobacter Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 238000007696 Kjeldahl method Methods 0.000 description 1
- 241000605159 Nitrobacter Species 0.000 description 1
- 241001495402 Nitrococcus Species 0.000 description 1
- 241000192147 Nitrosococcus Species 0.000 description 1
- 241000605122 Nitrosomonas Species 0.000 description 1
- 241001495394 Nitrosospira Species 0.000 description 1
- 241000192121 Nitrospira <genus> Species 0.000 description 1
- 229920001131 Pulp (paper) Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000005314 correlation function Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000010840 domestic wastewater Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Description
まず、脱窒槽4内から活性汚泥を採取する(ステップS10)。
次に、図2に示すステップS2において行われる決定係数に基づいて処理性能の指標となる指標細菌を、アンモニア酸化細菌、亜硝酸酸化細菌、硝酸還元細菌、亜硝酸還元細菌及び亜酸化窒素還元細菌からなる群から選択する選択工程の一例について説明する。
次に、図2に示すステップS3において行われる脱窒槽4内の亜硝酸還元細菌の数を測定する測定工程のサブルーチンを図7に示すフローチャートを用いて説明する。まず、脱窒槽4内から活性汚泥を採取する(ステップS21)。
次に、図2に示すステップS4において行われる脱窒槽4内の所定数以上の亜硝酸還元細菌を保持する保持工程の一例について説明する。
まず、脱窒槽4内から1mLの活性汚泥及び排水の混合物を採取した。次に、混合物中の活性汚泥を遠心分離により分離して、活性汚泥を得た。そして、活性汚泥に含まれる複数の種類の細菌の全DNAを、FastDNA SPIN kit for Soil(Qbiogene,Inc.製)を用い、製造業者の推奨する方法にしたがって抽出し、全DNAを11400ng得た。
nirS6R:CGTTGAACTT(A/G)CCGGT (配列番号2)
そして、得られた反応液を用いて、表1に示す条件でPCR法を実施した。
Nos1773R:AT(A/G)TCGATCA(A/G)CTG(T/C/G)TCGTT (配列番号4)
さらに、アンモニア酸化細菌についても、活性汚泥を採取する場所を脱窒槽4から硝化槽2に変更すること以外は同様に行い、決定係数R2を2×10-9と得た(図6(b)参照)。
amoA-2R:CCCCTC(G/T)G(C/G)AAAGCCTTCTTC (配列番号6)
(2)選択工程
上述した作成工程で作成されたそれぞれの細菌の決定係数に基づいて、決定係数R2が一番高かった亜硝酸還元細菌を指標細菌として選択した。
まず、脱窒槽4内から1mLの活性汚泥及び排水の混合物を採取した。次に、混合物中の活性汚泥を遠心分離により分離して、活性汚泥を得た。そして、活性汚泥に含まれる複数の種類の細菌の全DNAを、FastDNA SPIN kit for Soil(Qbiogene,Inc.製)を用い、製造業者の推奨する方法にしたがって抽出し、全DNAを12300ng得た。
nirS6R:CGTTGAACTT(A/G)CCGGT (配列番号2)
そして、得られた反応液を用いて、表1に示した条件でPCR法を実施した。
図6(a)から分離液の全窒素濃度(T-N)を2.0mg/L以下とするためには、亜硝酸還元細菌1ヶ当りの窒素負荷を3.2×10-8mg/(copies・日)以下となるよう亜硝酸還元細菌を保持すればよいことになる。そこで、処理性能を管理するために、亜硝酸還元細菌の数を測定する測定工程を10日置きに行った。そして、亜硝酸還元細菌1ヶ当りの窒素負荷が3.2×10-8mg/(copies・日)を超えるときには、亜硝酸還元細菌に適した基質を加えたり、pH、温度、酸化還元電位等を制御した。一方、亜硝酸還元細菌1ヶ当りの窒素負荷が3.2×10-8mg/(copies・日)以下であるときには、特に何もしなかった。
処理性能を管理し始めたときから、1、10、20、30及び40日目に、連絡管9から採取した分離液中の全窒素濃度T−Nを測定した。その結果を表2に示す。
2、22、32、34:硝化槽
4、21、31、33:脱窒槽
6:再曝気槽
8、23、35:沈殿槽
Claims (3)
- 硝化槽と脱窒槽とを有する水処理プロセスの処理性能を管理する水処理管理方法であって、
前記水処理プロセスは、複数の種類の細菌を用いて、水処理槽に供給される被処理水から窒素成分を除去するものであり、
硝化槽中のアンモニア酸化細菌又は亜硝酸酸化細菌、脱窒槽中の硝酸還元細菌、亜硝酸還元細菌及び亜酸化窒素還元細菌からなる群について、
単一の細菌の数と全窒素負荷との関係に基づいて、決定係数が最も高い細菌を処理性能の指標となる指標細菌として選択する選択工程と、
前記水処理槽内の指標細菌の数を測定する測定工程と、
前記水処理槽内に所定数以上の指標細菌を保持する保持工程とを含むことを特徴とする水処理管理方法。 - 前記測定工程では、定量PCR法を用いる、請求項1に記載の水処理管理方法。
- 前記被処理水は、火力発電所からの排水である、請求項1又は2に記載の水処理管理方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2005155509A JP4279802B2 (ja) | 2005-05-27 | 2005-05-27 | 水処理管理方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2005155509A JP4279802B2 (ja) | 2005-05-27 | 2005-05-27 | 水処理管理方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2006326522A JP2006326522A (ja) | 2006-12-07 |
JP4279802B2 true JP4279802B2 (ja) | 2009-06-17 |
Family
ID=37548806
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2005155509A Expired - Fee Related JP4279802B2 (ja) | 2005-05-27 | 2005-05-27 | 水処理管理方法 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP4279802B2 (ja) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4278701B1 (ja) * | 2007-06-19 | 2009-06-17 | 株式会社神鋼環境ソリューション | シミュレーション方法、シミュレーション装置、生物処理方法、ならびに、生物処理装置 |
JP5255818B2 (ja) * | 2007-11-05 | 2013-08-07 | 株式会社神鋼環境ソリューション | シミュレーション方法 |
JP5577017B2 (ja) * | 2007-10-04 | 2014-08-20 | 株式会社神鋼環境ソリューション | プライマー、プライマーセット、硝酸還元細菌定量方法、ならびに、生物処理方法 |
JP4922214B2 (ja) * | 2008-03-11 | 2012-04-25 | 株式会社東芝 | 水処理方法及び水処理装置 |
JP6530196B2 (ja) * | 2015-02-02 | 2019-06-12 | 学校法人 東洋大学 | アンモニア含有廃水の処理方法および処理装置 |
JP6501593B2 (ja) | 2015-04-03 | 2019-04-17 | 住友化学株式会社 | 予測ルール生成システム、予測システム、予測ルール生成方法及び予測方法 |
KR101995858B1 (ko) * | 2017-11-30 | 2019-07-04 | 한국에너지기술연구원 | 풍력발전 성능검사용 캡쳐 매트릭스 최적화 방법 |
-
2005
- 2005-05-27 JP JP2005155509A patent/JP4279802B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JP2006326522A (ja) | 2006-12-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP4279802B2 (ja) | 水処理管理方法 | |
Limpiyakorn et al. | Communities of ammonia-oxidizing bacteria in activated sludge of various sewage treatment plants in Tokyo | |
Bassin et al. | Effect of different salt adaptation strategies on the microbial diversity, activity, and settling of nitrifying sludge in sequencing batch reactors | |
Dong et al. | Ammonia-oxidizing bacterial community and nitrification rates in constructed wetlands treating swine wastewater | |
Lydmark et al. | Effects of environmental conditions on the nitrifying population dynamics in a pilot wastewater treatment plant | |
Burger et al. | Manganese removal and occurrence of manganese oxidizing bacteria in full-scale biofilters | |
Kim et al. | Comparison of conventional and integrated fixed‐film activated sludge systems: Attached‐and suspended‐growth functions and quantitative polymerase chain reaction measurements | |
Li et al. | Measuring nitrification inhibition by metals in wastewater treatment systems: Current state of science and fundamental research needs | |
Kurisu et al. | Effects of ammonium and nitrite on communities and populations of ammonia-oxidizing bacteria in laboratory-scale continuous-flow reactors | |
Bellucci et al. | Ammonia-oxidizing bacteria in wastewater | |
Sundberg et al. | Overland flow systems for treatment of landfill leachates—potential nitrification and structure of the ammonia-oxidising bacterial community during a growing season | |
Molina-Muñoz et al. | Microbial community structure and dynamics in a pilot-scale submerged membrane bioreactor aerobically treating domestic wastewater under real operation conditions | |
Cydzik-Kwiatkowska et al. | Impact of operational parameters on bacterial community in a full-scale municipal wastewater treatment plant | |
Ramsay et al. | Comprehensive analysis of the start-up period of a full-scale drinking water biofilter provides guidance for optimization | |
Weber et al. | Microbiology in treatment wetlands | |
Kim et al. | Identification of bacterial communities in conventional wastewater treatment sludge to inform inoculation of the anammox process | |
Dionisi et al. | Quantification of Nitrosomonas oligotropha and Nitrospira spp. using competitive polymerase chain reaction in bench‐scale wastewater treatment reactors operating at different solids retention times | |
Jaranowska et al. | Configuration of biological wastewater treatment line and influent composition as the main factors driving bacterial community structure of activated sludge | |
Pogue et al. | Impact of protozoan grazing on nitrification and the ammonia-and nitrite-oxidizing bacterial communities in activated sludge | |
EP2181966A1 (en) | Simulation method, simulation apparatus, biological treatment method and biological treatment apparatus | |
Hall et al. | Microbial quantification in activated sludge: the hits and misses | |
JP4278701B1 (ja) | シミュレーション方法、シミュレーション装置、生物処理方法、ならびに、生物処理装置 | |
Rowan et al. | A comparitive study of ammonia-oxidizing bacteria in lab-scale industrial wastewater treatment reactors | |
Ziembinska et al. | Molecular analysis of temporal changes of a bacterial community structure in activated sludge using denaturing gradient gel electrophoresis (DGGE) and fluorescent in situ hybridization (FISH) | |
Ziembinska et al. | Comparison of ammonia-oxidizing bacterial community structure in membrane-assisted bioreactors using PCR-DGGE and FISH |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20070726 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20070828 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20071022 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20081224 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20090216 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20090310 |
|
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20090312 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120319 Year of fee payment: 3 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 4279802 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120319 Year of fee payment: 3 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120319 Year of fee payment: 3 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130319 Year of fee payment: 4 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130319 Year of fee payment: 4 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130319 Year of fee payment: 4 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140319 Year of fee payment: 5 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |