JP4209462B1 - Ethanol production method - Google Patents

Abstract

The present invention provides a method for producing ethanol that is easy to operate and excellent in production efficiency.
A production process for producing ethanol by irradiating light from a light emitting diode in a sealed system containing a saccharified solid-liquid mixture, yeast and plant chloroplasts, and the ethanol produced from the system. A method for producing ethanol comprising a recovery step of recovering outside,
In the generating step,
a) a reaction in which a saccharified solid-liquid mixture is fermented with yeast to produce ethanol;
b) Reaction of photosynthesis of carbon dioxide generated during fermentation in a) by light irradiation of plant chloroplasts and light emitting diodes to sugar, and c) fermentation of sugar obtained in b) with yeast. The reaction for producing ethanol proceeds in parallel, and the saccharified solid / liquid mixture is obtained by pyrolyzing or hydrolyzing cellulose or hemicellulose to saccharify or contain rice, wheat, straw or corn containing starch. It is prepared by saccharification using straw, malt or an enzyme agent, and the plant chloroplast is a plant chloroplast of a seed plant, a fern plant, an algae, a moss plant, a bacterium, or a mixture thereof. The light emitted from the light emitting diode is light having a total light emission amount in the wavelength regions of 380 to 520 nm and 620 to 780 nm that is 70% or more of the light emission amount over the entire wavelength region. Method of manufacturing Lumpur.
[Selection figure] None

Description

  The present invention is a reaction in which a saccharified solid-liquid mixture is fermented by yeast to produce ethanol, and carbon dioxide generated during the fermentation is photo-synthesized by light irradiation of plant chloroplasts and light-emitting diodes to produce sugar. Ethanol that is easy to operate and excellent in production efficiency, characterized in that the three reactions of producing ethanol by fermenting the sugar obtained in the reaction with yeast, proceed in parallel in the same container It relates to the manufacturing method.

Japanese Patent Application Laid-Open No. 07-087986 discloses “microalgae culture means 1” for culturing microalgae that accumulate starch in cells by photosynthesis, and “microalgae concentration” for concentrating a culture solution containing algal bodies cultured by this means. Means 2 ", a method for producing ethanol including" holding means 3 "that maintains the slurry obtained by the means in a dark and anaerobic atmosphere while maintaining the pH in the range of 6.0 to 9.0 to produce ethanol. Has been.
In the above production method, “microalgae culture means 1” and “holding means 3” are completely independent separate processes, and a separate process of “microalgae concentration means 2” is required between the two processes. Therefore, the operation is complicated, and it cannot be said that the ethanol production method is always highly efficient.
In particular, since the “holding means 3” for generating ethanol needs to be kept in a dark and anaerobic atmosphere, it can be performed in parallel with the “microalgae culture means 1” that requires light irradiation in the same container. It was impossible.

Japanese Patent Application Laid-Open No. 2007-325564 discloses a method for inoculating koji mold and saccharifying a raw material subjected to a specific treatment to produce ethanol using rice as a starting material as a cereal and adding yeast to perform ethanol fermentation. In addition, a method of synthesizing and utilizing industrial methanol or the like by simply reusing carbon dioxide generated as a by-product during ethanol fermentation without letting it escape to the atmosphere is described.
Although the above method describes the reuse of carbon dioxide generated during ethanol fermentation, the carbon dioxide is converted into a sugar that is a raw material for ethanol fermentation by photosynthesis, and the sugar is converted into the above ethanol fermentation. There was no suggestion or description about performing in parallel in the same container.

In addition, a method for producing various organic compounds by using a light-emitting diode as a light source for photosynthesis to produce useful organic substances has been reported (for example, JP-A-10-113164, JP-A-2004-147541). , JP 2002-315569, Registered Utility Model No. 3073908, Tomonao Katsuta, Biochemical Engineering Study for Production of Useful Substances by Photobioreactor, 59th Annual Meeting of the Japanese Society for Biotechnology August 2, 2007, p. 107 (lecture number: 3D09-1)).
However, none of the above methods describe a specific method for producing sugar from carbon dioxide by photosynthesis.
Japanese Patent Application Laid-Open No. 07-087986 JP 2007-325564 A JP-A-10-113164 JP 2004-147461 A JP 2002-315569 A Registered Utility Model No. 3073908 Katsuta Tomonao, Biochemical engineering study for production of useful substances by photobioreactor, Abstracts of the 59th Annual Meeting of the Japanese Society for Biotechnology, August 2, 2007, p. 107 (lecture number: 3D09-1)

  The present invention is a reaction in which a saccharified solid-liquid mixture is fermented by yeast to produce ethanol, and carbon dioxide generated during the fermentation is photo-synthesized by light irradiation of plant chloroplasts and light-emitting diodes to produce sugar. Ethanol that is easy to operate and excellent in production efficiency, characterized in that the three reactions of producing ethanol by fermenting the sugar obtained in the reaction with yeast, proceed in parallel in the same container An object of the present invention is to provide a manufacturing method.

As a result of intensive studies to solve the above problems, the present inventors have conducted a simple operation of irradiating light from a light-emitting diode in a sealed system containing a saccharified solid-liquid mixture, yeast and plant chloroplasts. ,
a) a reaction in which a saccharified solid-liquid mixture is fermented with yeast to produce ethanol;
b) Reaction of photosynthesis of carbon dioxide generated during fermentation in a) by light irradiation of plant chloroplasts and light emitting diodes to sugar, and c) fermentation of sugar obtained in b) with yeast. It was found that the reaction for producing ethanol proceeded in parallel, and at that time, it was found that ethanol was efficiently produced by irradiating light in a specific wavelength region, and the present invention was completed.

That is, the present invention
(1) A production process for producing ethanol by irradiating light from a light-emitting diode in a sealed system containing a saccharified solid / liquid mixture, yeast and plant chloroplasts, and ethanol produced from the system outside the system A method of producing ethanol comprising a recovery step of recovering
In the generating step,
a) a reaction in which a saccharified solid-liquid mixture is fermented with yeast to produce ethanol;
b) Reaction of photosynthesis of carbon dioxide generated during fermentation in a) by light irradiation of plant chloroplasts and light emitting diodes to sugar, and c) fermentation of sugar obtained in b) with yeast. The reaction for producing ethanol proceeds in parallel, and the saccharified solid / liquid mixture is obtained by pyrolyzing or hydrolyzing cellulose or hemicellulose to saccharify or contain rice, wheat, straw or corn containing starch. It is prepared by saccharification using straw, malt or an enzyme agent, and the plant chloroplast is a plant chloroplast of a seed plant, a fern plant, an algae, a moss plant, a bacterium, or a mixture thereof. The light emitted from the light emitting diode is light having a total light emission amount in the wavelength regions of 380 to 520 nm and 620 to 780 nm that is 70% or more of the light emission amount over the entire wavelength region. The method of manufacturing Lumpur,
(2) The method for producing ethanol according to claim 1, wherein further carbon dioxide is added into the sealed system.
(3) The method for producing ethanol according to claim 1 or 2, wherein the recovery step is by distillation.
About.

  “The total emission amount in the wavelength region of 380 to 520 nm and 620 to 780 nm is 70% or more of the emission amount over the entire wavelength region” means that the emission spectrum of the light emitting diode used is measured and recorded. It means that a value indicating the sum of the spectrum areas in the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total spectrum area as a percentage is 70% or more.

The ethanol production method of the present invention produces sugar by a photosynthesis reaction using carbon dioxide generated in a sugar fermentation reaction as a raw material, and therefore does not discharge carbon dioxide. It can be said that this is a manufacturing method.
In addition, since ethanol is produced using sugar produced by the photosynthesis reaction as a raw material, the ethanol recovery rate is high compared to normal ethanol fermentation, and three reactions are simultaneously performed in one container. Since it can be performed, it is a very excellent ethanol production method that is simple and efficient in operation.
In a preferred embodiment of the present invention, additional carbon dioxide is added into the reaction system, whereby ethanol can be produced more efficiently.

  The ethanol production method of the present invention comprises a production step of producing ethanol by irradiating light from a light-emitting diode in a sealed system containing a saccharified solid-liquid mixture, yeast and plant chloroplast, and production from the system. A recovery step of recovering the produced ethanol out of the system.

The saccharified solid / liquid mixture used in the present invention is saccharified by thermally decomposing or hydrolyzing cellulose or hemicellulose, or saccharifying rice, wheat, straw or corn containing starch using straw, malt or an enzyme agent. It is prepared by.
Examples of cellulose or hemicellulose include cellulose or hemicellulose obtained from a plant body such as wood.
Saccharification of cellulose by thermal decomposition can be performed by a known method, for example, a method described in Japanese Patent No. 1400009.
The saccharification of cellulose or hemicellulose by hydrolysis can be performed by a known method, for example, a method of hydrolysis using dilute sulfuric acid described in JP-B 61-0444479.
In addition, the saccharified solid-liquid mixture uses starch-containing rice, wheat, straw, or corn, and if necessary, performs a step of removing the hull, pulverization or pulverization, etc., and adds straw, malt, or an enzyme agent to this Can be prepared.
Examples of the enzyme agent include amylase.
The saccharified solid / liquid mixture prepared above can be sterilized as necessary.

  The yeast used in the present invention is not particularly limited as long as it can produce ethanol, and specific examples include wine yeast, sake yeast, whiskey yeast, beer yeast and the like.

Plant chloroplasts used in the present invention include plant chloroplasts of seed plants, ferns, algae, moss plants, bacteria, or mixtures thereof.
Although it does not specifically limit as a seed plant, For example, a ginkgo, rice, etc. are preferable.
Although it does not specifically limit as a fern plant, For example, a cycad etc. are preferable.
Although it does not specifically limit as algae, For example, kawamozuku, gray algae, crypt algae, wakame, euglena algae, chloracunion algae, cyanobacteria, uremo etc. are preferable.
Although it does not specifically limit as a moss plant, For example, a sphagnum moss, a black moss, a scallop, a scale moss, a sphagnum, a hornbill, etc. are preferable.
Although it does not specifically limit as bacteria, For example, cyanobacteria (extracted from a sea lion) etc. are preferable.
Preferred plant chloroplasts include bacterial plant chloroplasts and cyanobacterial plant chloroplasts.

In general, the plant chloroplast used in the present invention preferably destroys the plant cell membrane so that the sugar produced by photosynthesis is efficiently fermented by the yeast in the solution. The destruction of the plant cell membrane can be performed, for example, by crushing the plant.
However, if the produced sugar is efficiently fermented without destroying the cell membrane, it is not necessary to destroy the cell membrane.

The sealed system is not particularly limited as long as it is a sealed container that can be irradiated with light and can cope with a volume change caused by the generated gas (carbon dioxide, oxygen). A container etc. are mentioned.
In the case of a glass container, light irradiation can be performed from the outside and / or the inside of the glass container, and in the case of a metal container, light irradiation can be performed from the inside of the container.
Moreover, it is preferable that the said container is equipped with the expansion-contraction site | part which can accommodate the produced | generated gas temporarily so that it can respond to the produced | generated gas.
The system is preferably stirrable.

The glass container is, for example, a glass tube installed around a light-emitting diode, and a solution containing a saccharified solid-liquid mixture, yeast, and plant chloroplasts is allowed to flow at a rate that allows an ethanol-producing reaction. It can also be a container capable of continuous reaction.
Further, the glass container is composed of, for example, two glass plates capable of irradiating light from a light emitting diode from both the front and back surfaces, and a saccharified solid-liquid mixture, yeast and plant chloroplasts are interposed between the glass plates. It may also be a container capable of continuous reaction by flowing the solution containing it at a rate that allows ethanol production reaction.

In the production method of ethanol of the present invention, the production step for producing ethanol is performed by irradiating the light of the light-emitting diode into the above-described sealed system. In the production step,
a) a reaction in which a saccharified solid-liquid mixture is fermented with yeast to produce ethanol;
b) Reaction of photosynthesis of carbon dioxide generated during fermentation in a) by light irradiation of plant chloroplasts and light emitting diodes to sugar, and c) fermentation of sugar obtained in b) with yeast. Three reactions of producing ethanol proceed in parallel in the closed system.

Ethanol and carbon dioxide are produced by the fermentation of yeast in a) above.
The produced carbon dioxide is converted to sugar by photosynthesis b) of the light of the plant chloroplast and the light emitting diode, and oxygen is generated at this time.
The sugar is not particularly limited as long as it can be used for ethanol fermentation, and examples thereof include sucrose and glucose.
The sugar produced in b) is fermented by yeast (reaction c)) to produce ethanol and carbon dioxide. The produced carbon dioxide is again ethanol through the photosynthesis of b) and alcohol fermentation of c). By repeating this cycle, almost all of the carbon dioxide is finally converted into ethanol and oxygen.
Therefore, the progress of the reaction can be grasped by measuring the oxygen concentration in the gas present in the system.
The reaction is preferably carried out until the oxygen concentration in the gas present in the system reaches 90% or more, and more preferably the reaction is carried out until the concentration reaches 95% or more.

The light emitted from the light-emitting diode used in the photosynthetic reaction is light in which the total light emission amount in the wavelength regions of 380 to 520 nm and 620 to 780 nm is 70% or more of the light emission amount over the entire wavelength region, and preferably 80% or more. And more preferably 90% or more.
When the above% is less than 70, ethanol production efficiency is lowered.
Further, light having a wavelength shorter than 380 nm is not substantially contained, that is, the light emission amount is 5% or less, preferably 3% or less, and preferably 0%.
“The total light emission amount in the wavelength range of 380 to 520 nm and 620 to 780 nm is 70% or more of the light emission amount over the entire wavelength region” means that the light emission spectrum of the light emitting diode used is measured and recorded. It means that a value indicating the sum of the spectrum areas in the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total spectrum area as a percentage is 70% or more.

In addition to the production | generation process which produces | generates ethanol, the manufacturing method of ethanol of this invention includes the collection | recovery process which collect | recovers the produced | generated ethanol out of the system.
The recovery step is not particularly limited as long as it is a method capable of separating ethanol from the reaction system. For example, a known method such as a distillation method or a membrane separation method can be used.
Moreover, when recovering ethanol by a distillation method, the container used for the distillation operation can be used as it is, and the reaction liquid can be transferred to another container for use.
A preferred embodiment of the present invention is a method for producing ethanol in which additional carbon dioxide is added into the sealed system.
By further adding carbon dioxide, the production efficiency of ethanol can be further improved.
The additional addition of carbon dioxide is achieved, for example, by connecting a carbon dioxide introduction tube to the reaction vessel or placing it in an atmosphere of carbon dioxide.
When adding carbon dioxide, the entire system is sealed so that the carbon dioxide does not flow out.

In a preferred embodiment of the present invention, the recovery step is achieved by distillation.
As the distillation method, any of simple distillation, azeotropic distillation, azeotropic distillation with addition of benzene, and the like can be selected according to the required purity of ethanol.

Production Example 1: Production of Saccharified Solid-Liquid Mixture (Rice) Add 6 g of amylase and 315 g of water to 330 g of rice flour purchased from a food store, stir with a mixer, boil and keep at 70 ° C. to 80 ° C. for 12 hours. By saccharifying rice flour, 651 g of a saccharified solid-liquid mixture (rice) was prepared.

Production Example 2: Production of saccharified solid / liquid mixture (wheat) 6 g of amylase and 315 g of water were added to 330 g of wheat flour purchased from a food store, stirred with a mixer, boiled in water and kept at 70 ° C. to 80 ° C. for 12 hours. By saccharifying wheat flour, 651 g of a saccharified solid-liquid mixture (wheat) was prepared.

Production Example 3: Production of saccharified solid-liquid mixture (corn) 6 g of amylase and 315 g of water were added to 330 g of corn flour purchased from a food store, stirred with a mixer, boiled and kept at 70 ° C. to 80 ° C. for 12 hours. By saccharifying corn flour, 651 g of a saccharified solid-liquid mixture (corn) was prepared.

結果：
デンプンを含む米を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｊ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が８０％となる発光ダイオードの光を照射すると、何れの植物葉緑体を用いた場合も、３．８ないし３．９ｍＬ程度のエタノールが製造された（実施例１ないし１０）が、これは、植物葉緑体を用いなかった場合（比較例１）に比して、約１．３倍の収量増であった。 Examples 1 to 10 and Comparative Example 1
217 g of the saccharified solid / liquid mixture (rice) produced in Production Example 1 and 3 g of yeast were stirred with a mixer to prepare 220 g of a saccharified solid / liquid mixture (rice) containing yeast.
The prepared yeast-containing saccharified solid / liquid mixture (rice) is divided into 11 100 mL beakers of 20 g each, 10 g of different plant pulverized products are added to each beaker in 1 g and stirred, and then each solution is made of transparent glass. Were injected into a 100 mL syringe (Examples 1 to 10).
For comparison, 20 g of a saccharified solid-liquid mixture (rice) containing yeast without adding plant pulverized material was injected into a 100 mL syringe (Comparative Example 1).
The eleven syringes prepared above are combined with 200 white light emitting diodes, 200 red light emitting diodes, 100 blue light emitting diodes and 100 green light emitting diodes. ) For 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 80%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 1.
In the table, symbols a to j represent the following plants, and “area ratio” is the ratio (%) of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectrum area recorded in the emission spectrum. Represents.
a: ginkgo leaf, b: cycad leaf, c: rice leaf, d: brookberry, e: gray algae, f: crypt algae, g: fresh seaweed, h: euglena algae, i: chloracunion algae, j : Blue-green algae

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to j), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation of light from a light emitting diode with a total light emission amount of 80% produced about 3.8 to 3.9 mL of ethanol using any plant chloroplast (Examples 1 to 10). However, this was about a 1.3 times increase in yield compared to the case where no plant chloroplast was used (Comparative Example 1).

結果：
デンプンを含む小麦を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｊ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が７７％となる発光ダイオードの光を照射すると、何れの植物葉緑体を用いた場合も、３．８ないし３．９ｍＬ程度のエタノールが製造された（実施例１１ないし２０）が、これは、植物葉緑体を用いなかった場合（比較例２）に比して、約１．３倍の収量増であった。 Examples 11 to 20 and Comparative Example 2
217 g of the saccharified solid / liquid mixture (wheat) produced in Production Example 2 and 3 g of yeast were stirred with a mixer to prepare 220 g of a saccharified solid / liquid mixture (wheat) containing yeast.
The prepared yeast-containing saccharified solid-liquid mixture (wheat) is divided into 11 100 mL beakers of 20 g each, 10 g of different plant pulverized products are added to each beaker by 1 g, and each solution is made of transparent glass. Were injected into a 100 mL syringe (Examples 11 to 20).
For comparison, 20 g of a saccharified solid-liquid mixture (wheat) containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 2).
The eleven syringes prepared above contain light from a total of 600 light emitting diodes composed of 200 white light emitting diodes, 200 red light emitting diodes, 100 yellow light emitting diodes and 100 indigo light emitting diodes (36 watts in total). / Hour) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was determined to be 77%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 2.
In the table, symbols a to j represent the same plants as shown above, and “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a sealed system containing a solid-liquid mixture prepared by saccharification of wheat containing starch, yeast and plant chloroplasts of various plants (a to j), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation with light from a light emitting diode with a total light emission amount of 77% produced about 3.8 to 3.9 mL of ethanol in any plant chloroplast (Examples 11 to 20). However, this was about a 1.3-fold increase in yield compared to the case where no plant chloroplast was used (Comparative Example 2).

結果：
デンプンを含むトウモロコシを糖化して調製された固液混合物、酵母及び種々の植物（ａないしｊ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が８５％となる発光ダイオードの光を照射すると、何れの植物葉緑体を用いた場合も、４．３ないし４．５ｍＬ程度のエタノールが製造された（実施例２１ないし３０）が、これは、植物葉緑体を用いなかった場合（比較例３）に比して、約１．３倍の収量増であった。 Examples 21 to 30 and Comparative Example 3
217 g of the saccharified solid / liquid mixture (corn) produced in Production Example 3 and 3 g of yeast were stirred with a mixer to prepare 220 g of a saccharified solid / liquid mixture (corn) containing yeast.
The prepared saccharified solid-liquid mixture (corn) containing yeast was divided into 11 100 mL beakers of 20 g each, 10 g of different plant pulverized products were added to each beaker by 1 g, and each solution was made of transparent glass. Into a 100 mL syringe (Examples 21 to 30).
For comparison, 20 g of a saccharified solid-liquid mixture (corn) containing yeast without adding plant pulverized material was injected into a 100 mL syringe (Comparative Example 3).
The eleven syringes prepared above contain 200 white light emitting diodes, 200 red light emitting diodes, 100 orange light emitting diodes, and 100 purple light emitting diodes, for a total of 600 light emitting diodes (36 watts / total). ) For 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 85%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 3.
In the table, symbols a to j represent the same plants as shown above, and “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of corn containing starch, yeast and plant chloroplasts of various plants (a to j), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation of light from a light emitting diode with a total light emission amount of 85% produced 4.3 to 4.5 mL of ethanol in any plant chloroplast (Examples 21 to 30). However, this was about a 1.3 times increase in yield compared to the case where no plant chloroplast was used (Comparative Example 3).

結果：
デンプンを含むトウモロコシを糖化して調製された固液混合物、酵母及び種々の植物（ａないしｊ）の植物葉緑体を含む密閉された系内に、更なる二酸化炭素を添加して、３８
０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が８５％となる発光ダイオードの光を照射すると、何れの植物葉緑体を用いた場合も、５．３ないし５．６ｍＬ程度のエタノールが製造された（実施例３１ないし４０）が、これは、植物葉緑体を用いなかった場合（比較例４）に比して、約１．６倍の収量増であり、また、更なる二酸化炭素を添加していない実施例２１ないし３０に比して、約１．２４倍の収量増であった。 Examples 31 to 40 and Comparative Example 4
217 g of the saccharified solid / liquid mixture (corn) produced in Production Example 3 and 3 g of yeast were stirred with a mixer to prepare 220 g of a saccharified solid / liquid mixture (corn) containing yeast.
The prepared yeast-containing saccharified solid-liquid mixture (corn) is divided into 11 100 mL beakers each containing 20 g, 10 g of different plant pulverized products are added to each beaker and stirred, and each beaker is sealed in a box. I put it in.
A fermentation experiment was conducted separately from the above experiment, and carbon dioxide released from the fermentation experiment was introduced into each beaker through a tube so as to be mixed at the liquid level of each beaker in the sealed box (Examples 31 to 40). ).
As a comparison, a beaker containing 20 g of a saccharified solid-liquid mixture (corn) containing yeast without containing plant pulverized material was placed in the sealed box (Comparative Example 4).
In each of the above beakers, light of a total of 600 light emitting diodes composed of 200 white light emitting diodes, 200 red light emitting diodes, 100 orange light emitting diodes and 100 purple light emitting diodes (total of 36 watts / hour) is 24. Irradiated for hours. The emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas in the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 85%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 4.
In the table, symbols a to j represent the same plants as shown above, and “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
Additional carbon dioxide was added into a closed system containing a solid-liquid mixture prepared by saccharification of corn containing starch, yeast and plant chloroplasts of various plants (a to j), and 38
When irradiated with light from a light emitting diode whose total light emission in the wavelength range of 0 to 520 nm and 620 to 780 nm is 85%, ethanol of about 5.3 to 5.6 mL is used in any plant chloroplast. Was produced (Examples 31 to 40), which was about a 1.6-fold increase in yield compared to the case where no plant chloroplast was used (Comparative Example 4). The yield was increased about 1.24 times compared to Examples 21 to 30 in which carbon dioxide was not added.

Production Example 4: Production of saccharified solid-liquid mixture (rice) 10 g of amylase (mixture of alpha amylase and glucoamylase) and 525 g of water were added to 530 g of rice flour purchased from a food store, stirred with a mixer, then boiled in water, and 70 ° C or By maintaining the temperature at 80 ° C. and saccharifying the rice flour over 12 hours, 1065 g of a saccharified solid-liquid mixture (rice) was prepared.

結果：
デンプンを含む米を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｑ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が８０％となる発光ダイオードの光を照射すると、何れの植物葉緑体を用いた場合も、３．８ないし３．９ｍＬ程度のエタノールが製造された（実施例４１ないし５７）が、これは、植物葉緑体を用いなかった場合（比較例５）に比して、約１．３倍の収量増であった。 Examples 41 to 57 and Comparative Example 5
The saccharified solid-liquid mixture (rice) 355g and yeast 5g which were manufactured in manufacture example 4 were stirred with the mixer, and the saccharified solid-liquid mixture (rice) 360g containing yeast was prepared.
The prepared yeast-containing saccharified solid-liquid mixture (rice) was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred, and then each solution was made of transparent glass. Into a 100 mL syringe (Examples 41 to 57).
As a comparison, 20 g of a saccharified solid / liquid mixture (rice) containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 5).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 white light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 80%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 5.
In the table, the symbols a to q represent the following plants, and the “area ratio” is the ratio (%) of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents.
a: ginkgo leaf, b: cycad leaf, c: rice leaf, d: brookberry, e: gray algae, f: crypt algae, g: fresh seaweed, h: euglena algae, i: chloracunion algae, j : Blue-green algae, k: sphagnum, l: black moss, m: sphagnum, n: scale moss, o: sphagnum, p: hornwort, q: cyanobacteria (extracted from blue seaweed)

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation of light from a light emitting diode with a total light emission amount of 80% produced about 3.8 to 3.9 mL of ethanol in any plant chloroplast (Examples 41 to 57). However, this was about a 1.3 times increase in yield compared to the case where no plant chloroplast was used (Comparative Example 5).

結果：
デンプンを含む米を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｑ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が７４％となる発光ダイオードの光を照射すると、何れの植物葉緑体を用いた場合も、３．８ないし３．９ｍＬ程度のエタノールが製造された（実施例５８ないし７４）が、これは、植物葉緑体を用いなかった場合（比較例６）に比して、約１．３倍の収量増であった。 Examples 58 to 74 and Comparative Example 6
The saccharified solid-liquid mixture (rice) 355g and yeast 5g which were manufactured in manufacture example 4 were stirred with the mixer, and the saccharified solid-liquid mixture (rice) 360g containing yeast was prepared.
The prepared yeast-containing saccharified solid-liquid mixture (rice) was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred, and then each solution was made of transparent glass. Into a 100 mL syringe (Examples 58-74).
As a comparison, 20 g of a saccharified solid-liquid mixture (rice) containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 6).
The 18 syringes prepared above were irradiated with light (total 36 watts / hour) from a total of 600 light emitting diodes composed of 200 red light emitting diodes, 200 blue light emitting diodes and 200 green light emitting diodes for 24 hours. did. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 74%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 6.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation of light from a light emitting diode with a total light emission amount of 74% produced about 3.8 to 3.9 mL of ethanol in any plant chloroplast (Examples 58 to 74). However, this was an increase in yield of about 1.3 times compared to the case where no plant chloroplast was used (Comparative Example 6).

結果：
デンプンを含む米を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｑ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が１００％となる発光ダイオードの光を照射すると、何れの植物葉緑体を用いた場合も、３．８ないし３．９ｍＬ程度のエタノールが製造された（実施例７５ないし９１）が、これは、植物葉緑体を用いなかった場合（比較例７）に比して、約１．３倍の収量増であった。 Examples 75 to 91 and Comparative Example 7
The saccharified solid-liquid mixture (rice) 355g and yeast 5g which were manufactured in manufacture example 4 were stirred with the mixer, and the saccharified solid-liquid mixture (rice) 360g containing yeast was prepared.
The prepared yeast-containing saccharified solid-liquid mixture (rice) was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred, and then each solution was made of transparent glass. Into a 100 mL syringe (Examples 75 to 91).
For comparison, 20 g of a saccharified solid-liquid mixture (rice) containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 7).
The 18 syringes prepared above were irradiated with light (total 36 watts / hour) of a total of 600 light emitting diodes composed of 300 red light emitting diodes and 300 blue light emitting diodes for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 100%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 7.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation of light from a light emitting diode with a total light emission amount of 100% produced about 3.8 to 3.9 mL of ethanol in any plant chloroplast (Examples 75 to 91). However, this was an increase in yield of about 1.3 times compared to the case where no plant chloroplast was used (Comparative Example 7).

結果：
デンプンを含む米を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｑ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が６０％となる発光ダイオードの光を照射すると、ｑのシアノバクテリアの場合（比較例２４）を除き、何れの植物葉緑体を用いた場合も、３．３ないし３．４ｍＬ程度のエタノールが製造された（比較例８ないし２３）が、これは、植物葉緑体を用いなかった場合（比較例２５）に比して、約１．１倍程度の収量増しかなかった。ｑのシアノバクテリアの場合（比較例２４）は、比較例２５に比して、約１．２３倍の収量増があった。 Comparative Examples 8 to 25
The saccharified solid-liquid mixture (rice) 355g and yeast 5g which were manufactured in manufacture example 4 were stirred with the mixer, and the saccharified solid-liquid mixture (rice) 360g containing yeast was prepared.
The prepared yeast-containing saccharified solid-liquid mixture (rice) was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred, and then each solution was made of transparent glass. Into a 100 mL syringe (Comparative Examples 8 to 24).
As a comparison, 20 g of a saccharified solid-liquid mixture (rice) containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 25).
The 18 syringes prepared above were irradiated with light (total 36 watts / hour) of a total of 600 light emitting diodes composed of 300 blue light emitting diodes and 300 green light emitting diodes for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 60%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 8.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. When irradiated with light from a light emitting diode with a total light emission amount of 60%, except for the case of q cyanobacteria (Comparative Example 24), 3.3 to 3.4 mL when using any plant chloroplast. About ethanol was produced (Comparative Examples 8 to 23), but this was only about a 1.1-fold increase in yield compared to the case where no plant chloroplast was used (Comparative Example 25). . In the case of q cyanobacteria (Comparative Example 24), the yield increased about 1.23 times compared to Comparative Example 25.

結果：
デンプンを含む米を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｑ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が６０％となる発光ダイオードの光を照射すると、ｑのシアノバクテリアの場合（比較例４２）を除き、何れの植物葉緑体を用いた場合も、３．２ないし３．３ｍＬ程度のエタノールが製造された（比較例２６ないし４１）が、これは、植物葉緑体を用いなかった場合（比較例４３）に比して、約１．１倍程度の収量増しかなかった。ｑのシアノバクテリアの場合（比較例４２）は、比較例４３に比して、約１．２２倍の収量増があった。 Comparative Examples 26 to 43
The saccharified solid-liquid mixture (rice) 355g and yeast 5g which were manufactured in manufacture example 4 were stirred with the mixer, and the saccharified solid-liquid mixture (rice) 360g containing yeast was prepared.
The prepared yeast-containing saccharified solid-liquid mixture (rice) was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred, and then each solution was made of transparent glass. Into a 100 mL syringe (Comparative Examples 26 to 42).
As a comparison, 20 g of a saccharified solid / liquid mixture (rice) containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 43).
The 18 syringes prepared above were irradiated with light (total 36 watts / hour) of 600 light-emitting diodes composed of 300 red light-emitting diodes and 300 green light-emitting diodes for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 60%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 9.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. When irradiated with light from a light emitting diode whose total light emission amount is 60%, except for the case of q cyanobacteria (Comparative Example 42), 3.2 to 3.3 mL of any plant chloroplast is used. About ethanol was produced (Comparative Examples 26 to 41), but this was only about 1.1 times the yield increase compared to the case where no plant chloroplast was used (Comparative Example 43). . In the case of q cyanobacteria (Comparative Example 42), the yield increased about 1.22 times compared to Comparative Example 43.

結果：
デンプンを含む米を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｑ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が１００％となる発光ダイオードの光を照射すると、何れの植物葉緑体を用いた場合も、３．８ないし３．９ｍＬ程度のエタノールが製造された（実施例９２ないし１０８）が、これは、植物葉緑体を用いなかった場合（比較例４４）に比して、約１．３倍の収量増であった。 Examples 92 to 108 and Comparative Example 44
The saccharified solid-liquid mixture (rice) 355g and yeast 5g which were manufactured in manufacture example 4 were stirred with the mixer, and the saccharified solid-liquid mixture (rice) 360g containing yeast was prepared.
The prepared yeast-containing saccharified solid-liquid mixture (rice) was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred, and then each solution was made of transparent glass. Into a 100 mL syringe (Examples 92 to 108).
As a comparison, 20 g of a saccharified solid-liquid mixture (rice) containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 44).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 blue light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 100%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 10.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation with light from a light emitting diode with a total light emission amount of 100% produced about 3.8 to 3.9 mL of ethanol in any plant chloroplast (Examples 92 to 108). However, this was about a 1.3-fold increase in yield compared to the case where no plant chloroplast was used (Comparative Example 44).

結果：
デンプンを含む米を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｑ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が１００％となる発光ダイオードの光を照射すると、何れの植物葉緑体を用いた場合も、３．８ないし３．９ｍＬ程度のエタノールが製造された（実施例１０９ないし１２５）が、これは、植物葉緑体を用いなかった場合（比較例４５）に比して、約１．３倍の収量増であった。 Examples 109 to 125 and Comparative Example 45
The saccharified solid-liquid mixture (rice) 355g and yeast 5g which were manufactured in manufacture example 4 were stirred with the mixer, and the saccharified solid-liquid mixture (rice) 360g containing yeast was prepared.
The prepared yeast-containing saccharified solid-liquid mixture (rice) was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred, and then each solution was made of transparent glass. Into a 100 mL syringe (Examples 109 to 125).
For comparison, 20 g of a saccharified solid-liquid mixture (rice) containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 45).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 red light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 100%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 11.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation of light from a light emitting diode with a total light emission amount of 100% produced about 3.8 to 3.9 mL of ethanol in any plant chloroplast (Examples 109 to 125). However, this was about a 1.3-fold increase in yield compared to the case where no plant chloroplast was used (Comparative Example 45).

結果：
デンプンを含む米を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｑ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が２０％となる発光ダイオードの光を照射すると、ｑのシアノバクテリアの場合（比較例６２）を除き、何れの植物葉緑体を用いた場合も、３．４ないし３．５ｍＬ程度のエタノールが製造された（比較例４６ないし６１）が、これは、植物葉緑体を用いなかった場合（比較例６３）に比して、約１．１５倍程度の収量増しかなかった。ｑのシアノバクテリアの場合（比較例６２）は、比較例６３に比して、約１．２７倍の収量増があった。 Comparative Examples 46 to 63
The saccharified solid-liquid mixture (rice) 355g and yeast 5g which were manufactured in manufacture example 4 were stirred with the mixer, and the saccharified solid-liquid mixture (rice) 360g containing yeast was prepared.
The prepared yeast-containing saccharified solid-liquid mixture (rice) was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred, and then each solution was made of transparent glass. Into a 100 mL syringe (Comparative Examples 46 to 62).
For comparison, 20 g of a saccharified solid-liquid mixture (rice) containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 63).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 green light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 20%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 12.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. When irradiated with light from a light emitting diode with a total light emission amount of 20%, except for the case of q cyanobacteria (Comparative Example 62), 3.4 to 3.5 mL when any plant chloroplast is used. About ethanol was produced (Comparative Examples 46 to 61), but this was only about 1.15 times higher yield than when no plant chloroplast was used (Comparative Example 63). . In the case of q cyanobacteria (Comparative Example 62), the yield increased about 1.27 times compared to Comparative Example 63.

結果：
デンプンを含む米を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｑ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が０％となる発光ダイオードの光を照射すると、ｑのシアノバクテリアの場合（比較例８０）を除き、何れの植物葉緑体を用いた場合も、３．２ないし３．４ｍＬ程度のエタノールが製造された（比較例６４ないし７９）が、これは、植物葉緑体を用いなかった場合（比較例８１）に比して、約１．１倍程度の収量増しかなかった。ｑのシアノバクテリアの場合（比較例８０）は、比較例８１に比して、約１．２２倍の収量増があった。 Comparative Examples 64 to 81
The saccharified solid-liquid mixture (rice) 355g and yeast 5g which were manufactured in manufacture example 4 were stirred with the mixer, and the saccharified solid-liquid mixture (rice) 360g containing yeast was prepared.
The prepared yeast-containing saccharified solid-liquid mixture (rice) was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred, and then each solution was made of transparent glass. Into a 100 mL syringe (Comparative Examples 64 to 80).
For comparison, 20 g of a saccharified solid-liquid mixture (rice) containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 81).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 yellow light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 0%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 13.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. When irradiated with light from a light emitting diode with a total light emission amount of 0%, except for the case of q cyanobacteria (Comparative Example 80), any plant chloroplast is used in an amount of 3.2 to 3.4 mL. About ethanol was produced (Comparative Examples 64 to 79), but this was only about a 1.1-fold increase in yield compared to the case where no plant chloroplast was used (Comparative Example 81). . In the case of q cyanobacteria (Comparative Example 80), the yield increased about 1.22 times compared to Comparative Example 81.

結果：
デンプンを含む米を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｑ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が１００％となる発光ダイオードの光を照射すると、ｑのシアノバクテリアの場合（実施例１４２）を除き、何れの植物葉緑体を用いた場合も、３．８ないし４．０ｍＬ程度のエタノールが製造された（実施例１２６ないし１４１）が、これは、植物葉緑体を用いなかった場合（比較例８２）に比して、約１．３倍の収量増であった。ｑのシアノバクテリアの場合（実施例１４２）は、比較例８２に比して約１．４７倍の収量増であった。 Examples 126 to 142 and Comparative Example 82
The saccharified solid-liquid mixture (rice) 355g and yeast 5g which were manufactured in manufacture example 4 were stirred with the mixer, and the saccharified solid-liquid mixture (rice) 360g containing yeast was prepared.
The prepared yeast-containing saccharified solid-liquid mixture (rice) was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred, and then each solution was made of transparent glass. Into a 100 mL syringe (Examples 126 to 142).
For comparison, 20 g of a saccharified solid-liquid mixture (rice) containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 82).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 purple light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 100%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 14.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. When irradiated with light from a light emitting diode with a total light emission amount of 100%, except for the case of q cyanobacteria (Example 142), 3.8 to 4.0 mL in any plant chloroplast. About ethanol was produced (Examples 126 to 141), which was about a 1.3-fold increase in yield compared to the case where no plant chloroplast was used (Comparative Example 82). In the case of q cyanobacteria (Example 142), the yield was increased about 1.47 times as compared with Comparative Example 82.

結果：
デンプンを含む米を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｑ）の植物葉緑体を含む密閉された系内に、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が５０％となる発光ダイオードの光を照射すると、ｑのシアノバクテリアの場合（比較例９９）を除き、何れの植物葉緑体を用いた場合も、３．４ないし３．５ｍＬ程度のエタノールが製造された（比較例８３ないし９８）が、これは、植物葉緑体を用いなかった場合（比較例１００）に比して、約１．１５倍程度の収量増しかなかった。ｑのシアノバクテリアの場合（比較例９９）は、比較例１００に比して、約１．２６倍の収量増があった。 Comparative Examples 83 to 100
The saccharified solid-liquid mixture (rice) 355g and yeast 5g which were manufactured in manufacture example 4 were stirred with the mixer, and the saccharified solid-liquid mixture (rice) 360g containing yeast was prepared.
The prepared yeast-containing saccharified solid-liquid mixture (rice) was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred, and then each solution was made of transparent glass. Into a 100 mL syringe (Comparative Examples 83 to 99).
For comparison, 20 g of a saccharified solid-liquid mixture (rice) containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 100).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 orange light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 50%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 15.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. When irradiated with light from a light emitting diode with a total light emission amount of 50%, except for the case of q cyanobacteria (Comparative Example 99), 3.4 to 3.5 mL when any plant chloroplast is used. About ethanol was produced (Comparative Examples 83 to 98), but this was only about 1.15 times higher yield than when no plant chloroplast was used (Comparative Example 100). . In the case of q cyanobacteria (Comparative Example 99), the yield increased about 1.26 times compared to Comparative Example 100.

結果：
デンプンを含む米を糖化して調製された固液混合物、酵母及び種々の植物（ａないしｑ）の植物葉緑体を含む密閉された系内に、更なる二酸化炭素を添加して、３８０ないし５２０ｎｍ及び６２０ないし７８０ｎｍの波長領域の発光量の合計が８０％となる発光ダイオードの光を照射すると、ｑのシアノバクテリアの場合（実施例１５９）を除き、何れの植物葉緑体を用いた場合も、５．５ないし５．６ｍＬ程度のエタノールが製造された（実施例１４３ないし１５８）が、これは、植物葉緑体を用いなかった場合（比較例１０１）に比して、約１．７倍の収量増であり、また、更なる二酸化炭素を添加していない実施例
４１ないし５７に比して、約１．４倍の収量増であった。また、ｑのシアノバクテリアの場合（実施例１５９）は、更なる二酸化炭素を添加していない実施例５７に比して約２倍の収量増であった。 Examples 143 to 159 and Comparative Example 101
The saccharified solid-liquid mixture (rice) 355g and yeast 5g which were manufactured in manufacture example 4 were stirred with the mixer, and the saccharified solid-liquid mixture (rice) 360g containing yeast was prepared.
The prepared yeast-containing saccharified solid-liquid mixture (rice) is divided into 18 100 mL beakers of 20 g each, and 17 types of different plant pulverized products are added to each beaker by 1 g and stirred, and then each beaker is sealed in a box. I put it in.
A fermentation experiment was conducted separately from the above experiment, and carbon dioxide released from the fermentation experiment was introduced into each beaker through a tube so as to be mixed at the liquid level of each beaker in the sealed box (Examples 143 to 159). ).
For comparison, a beaker containing 20 g of a saccharified solid-liquid mixture (rice) containing yeast without containing plant pulverized material was placed in the sealed box (Comparative Example 101).
Each beaker was irradiated with light from a light emitting diode composed of 600 white light emitting diodes (36 watts / hour in total) for 24 hours. The emission spectrum of the light-emitting diode was measured, and the ratio of the spectral area in the wavelength region of 380 to 520 nm and 620 to 780 nm to the total recorded spectral area was determined to be 80%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 16.
In the table, symbols a to j represent the same plants as shown above, and “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), additional carbon dioxide is added and 380 to When irradiating light from a light emitting diode with a total light emission amount of 80% in the wavelength region of 520 nm and 620 to 780 nm, except for the case of q cyanobacteria (Example 159), when using any plant chloroplast In addition, about 5.5 to 5.6 mL of ethanol was produced (Examples 143 to 158), which was about 1.times. Compared to the case where no plant chloroplast was used (Comparative Example 101). The yield increased by 7 times, and the yield increased by about 1.4 times compared to Examples 41 to 57 in which no additional carbon dioxide was added. In addition, in the case of q cyanobacteria (Example 159), the yield was increased about twice as compared with Example 57 in which no additional carbon dioxide was added.

The ethanol production method of the present invention does not emit carbon dioxide, and thus is said to be a useful method in terms of CO ₂ reduction. Moreover, since the manufacturing method of ethanol of this invention can obtain oxygen as a by-product, this can also be utilized as an effective resource.
In addition, since the ethanol production method of the present invention can take in carbon dioxide from the outside and convert it into ethanol, it can also be a useful ethanol production method from the viewpoint of fixing carbon dioxide. .

Claims (3)

A production process for producing ethanol by irradiating light from a light emitting diode in a sealed system containing a saccharified solid-liquid mixture, yeast and plant chloroplasts, and recovering the ethanol produced from the system outside the system A method for producing ethanol comprising a recovery step,
In the generating step,
a) a reaction in which a saccharified solid-liquid mixture is fermented with yeast to produce ethanol;
b) Reaction of photosynthesis of carbon dioxide generated during fermentation in a) by light irradiation of plant chloroplasts and light emitting diodes to sugar, and c) fermentation of sugar obtained in b) with yeast. The reaction for producing ethanol proceeds in parallel, and the saccharified solid / liquid mixture is obtained by pyrolyzing or hydrolyzing cellulose or hemicellulose to saccharify or contain rice, wheat, straw or corn containing starch. It is prepared by saccharification using straw, malt or an enzyme agent, and the plant chloroplast is a plant chloroplast of a seed plant, a fern plant, an algae, a moss plant, a bacterium, or a mixture thereof. The light emitted from the light emitting diode is light having a total light emission amount in the wavelength regions of 380 to 520 nm and 620 to 780 nm that is 70% or more of the light emission amount over the entire wavelength region. Method of manufacturing Lumpur. The method for producing ethanol according to claim 1, wherein further carbon dioxide is added into the sealed system. The method for producing ethanol according to claim 1 or 2, wherein the recovery step is by distillation.