JP4186296B2 - Egg collection method and apparatus - Google Patents

Description

[0001]
[Technical field to which the invention belongs]
The present invention relates to a method and apparatus for collecting eggs and, for example, relates to a method and apparatus for collecting eggs and collecting the chorioallantoic fluid after inoculating virus and the like into the chorioallantoic fluid of chicken eggs and culturing for a predetermined period of time. Is.
[0002]
[Prior art]
It has been conventionally practiced to produce a vaccine by inoculating virus and the like into chorioallantoic fluid in a growing chicken egg grown for a certain period of time after laying eggs and culturing for a predetermined period of time. As shown in FIG. 7, the growing chicken egg A grown for a certain period of time has inner chorioallantoic fluid B, yolk C, fetus D, etc. covered with a membrane, respectively. After that, the chorioallantoic membrane E is broken and the chorioallantoic fluid B is collected and used. At this time, if yolk C or other liquid is mixed in chorioallantoic fluid B, it cannot be used as a vaccine raw material.
[0003]
In order to collect only chorioallantoic fluid B from growing chicken egg A that has been inoculated with viruses and the like, conventionally, after placing egg A with the airway F side facing upward and cutting the upper part of eggshell G The liquid was collected by inserting a nozzle from above and performing vacuum suction.
[0004]
[Problems to be solved by the invention]
There is a limit to the size of the eggshell that can be cut due to the shape of the contents of the hen egg from which the chorioallantoic fluid is collected. It was impossible to insert. Accordingly, there is a problem that the chorioallantoic fluid below cannot be collected only by suctioning from above by the nozzle, and the yield is poor and the efficiency is not good.
[0005]
The present invention has been made to solve the above-mentioned problems, and a method and a liquid collection method from a chicken egg that can efficiently recover chorioallantoic fluid from the egg and reduce the amount of egg yolk and the like. The object is to provide an apparatus.
[0006]
[Means for Solving the Problems]
In the egg collection method according to the present invention, the egg cut from the upper part of the eggshell is positioned with the air chamber side facing upward, and the cut portion disposed above the egg is broken at the lower end to break the chorioallantoic membrane of the egg. The chorioallantoic membrane cutter and a pressing member provided inside the chorioallantoic membrane cutter and having a tip projecting downward from the cut portion are lowered to break the chorioallantoic membrane in the egg, and by the pressing member The chorioallantoic fluid in the egg is collected while holding the fetus.
[0007]
Further, in the liquid collecting method according to the second invention, the chorioallantoic membrane in the egg is broken by the chorioallantoic cutter, and the chorioallantoic fluid is collected by tilting the chicken egg at a predetermined angle while holding the fetus by the pressing member. It is what you do.
[0008]
Further, in the liquid collecting method according to the third invention, the chorioallantoic membrane in the chicken egg is broken by the chorioallantoic membrane cutter, and the fetus is held by the pressing member, and the suction provided in the vicinity of the chorioallantoic membrane cutter and the pressing member. The chorioallantoic fluid in the hen's egg is collected by aspiration by means.
[0009]
In addition, the egg collection device according to the fourth aspect of the present invention is disposed above the egg on the placement plate, a placing plate for positioning and placing the egg, a turning means for turning the placing plate, and the like. A chorioallantoic membrane cutter having a cut portion that breaks the chorioallantoic membrane of the egg at the lower end, and a holding member that is provided inside the chorioallantoic membrane cutter and holds the fetus in the egg by the lower end protruding from the chorioallantoic membrane cutter; Elevating means for elevating and lowering the chorioallantoic membrane cutter and the pressing member, and a chorioallantoic fluid collecting unit disposed below the mounting plate, and lowering the chorioallantoic membrane cutter and the pressing member to Is cut and inserted into the chicken egg placed on the mounting plate, the chorioallantoic membrane is broken, and the mounting plate is rotated by a predetermined angle while holding the fetus, and the chorioallantoic fluid in the chicken egg is collected in the collecting part It is what you do.
[0010]
Further, the liquid collecting apparatus according to the fifth invention includes suction means arranged in the vicinity of the chorioallantoic membrane cutter and the pressing member, and the upper portion is cut by lowering the chorioallantoic membrane cutter and the pressing member. Then, it is inserted into a chicken egg placed on a placing plate, the chorioallantoic membrane is broken, and the chorioallantoic fluid in the egg is collected by sucking it with suction means while holding the fetus.
[0011]
DETAILED DESCRIPTION OF THE INVENTION
Growing chicken eggs for liquid collection inoculated with viruses or the like are arranged so that the air chambers face upward, and the eggshells of the air chamber portions are cut. Thus, the chicken egg in which the upper part of the eggshell is cut to expose the chorioallantoic membrane is positioned and arranged on the mounting plate. This mounting plate can be tilted by the rotating means. Above the positioned egg, a chorioallantoic cutter with a blade (cut part) that breaks the chorioallantoic membrane at the lower end, and the lower end protrudes below the cut part inside the chorioallantoic cutter. The middle rod (pressing member) is disposed so as to be able to move up and down, and the chorioallantoic membrane cutter and the pressing member are lowered. After the pressing member descends while pressing the chorioallantoic membrane, the cut portion of the chorioallantoic membrane cutter breaks the chorioallantoic membrane. When the mounting plate on which the chicken egg is placed is tilted by the rotating means while the pressing member holds the egg yolk and the fetus inside the egg, the egg yolk and the fetus do not flow out and only the chorioallantoic fluid flows out and is collected. The chorioallantoic fluid below the fetus and egg yolk can also flow out of these areas and be collected.
[0012]
Further, the rotating means for tilting the mounting plate can be omitted, and the chorioallantoic fluid can be sucked by the suction means provided near the chorioallantoic membrane cutter and the pressing member. Also in this case, after the chorioallantoic membrane cutter is broken by the lowered chorioallantoic membrane cutter, it is sucked by the suction means while holding the fetus and yolk by the pressing member, so the chorioallantoic fluid below the fetus etc. is also pushed out and It is sucked and collected from the suction means.
[0013]
【Example】
Hereinafter, the present invention will be described with reference to embodiments shown in the drawings. FIG. 1 shows an egg liquid collecting apparatus according to an embodiment of the present invention, and a first cylinder 4 is provided on a mounting plate 2 on which an egg A is placed. A second cylinder 8 is fixed on the upward operating rod 6 of the first cylinder 4. Further, a downward chorioallantoic membrane cutter 14 and a pressing member 16 are integrally attached to the operating rod 10 of the second cylinder 8 via a support plate 12. The second cylinder 8, the chorioallantoic membrane cutter 14, and the pressing member 16 are moved up and down as a whole by the operation of the first cylinder 4. The chorioallantoic membrane cutter 14 and the pressing member 16 are moved up and down by the operation of the second cylinder 8. A guide member 18 is fixed to the lower portion of the second cylinder 8 and guides the elevation of the chorioallantoic membrane cutter 14 and the pressing member 16 relative to the second cylinder 8. The first cylinder 4 extends and contracts the rod 6 with a large stroke, and the second cylinder 8 expands and contracts the rod 10 with a small stroke.
[0014]
One side 2a of the mounting plate 2 is fixed to a horizontal rotating shaft 20, and can be rotated around the rotating shaft 20 by a rotating means (not shown). In this embodiment, the mounting plate 2 can be inclined at a predetermined angle by rotating in the arrow direction R of FIG.
[0015]
The chorioallantoic membrane cutter 14 has a cylindrical shape, and a serrated cut portion for breaking the chorioallantoic membrane E in the egg A is provided at the tip (lower end) 14a (see FIG. 2). . A rod-shaped pressing member 16 is fitted in the chorioallantoic membrane cutter 14. An annular space 22 is formed between the outer peripheral surface of the pressing member 16 and the inner peripheral surface of the chorioallantoic membrane cutter 14. In addition, the pressing member 16 has a tip end (lower end) 16 a extending downward from a lower end portion (cut portion) 14 a of the chorioallantoic membrane cutter 14. It is positioned.
[0016]
On the mounting plate 2, there is a positioning part P for placing and positioning the egg A accommodated in the carrier 26. Above the egg A positioned in the positioning part P, the chorioallantoic membrane cutter 14 and the pressing member 16 are installed such that the center O ₁ and the center O _{2 of the} egg coincide with each other.
[0017]
A liquid collection method using the egg egg liquid collection apparatus according to the above configuration will be described. The first cylinder 4 extends the operating rod 6 to raise the second cylinder 8 and the chorioallantoic membrane cutter 14 and the pressing member 16 attached to the second cylinder 8 as a whole. Further, the second cylinder 8 contracts the operating rod 10 so that the chorioallantoic membrane cutter 14 and the pressing member 16 are lowered with respect to the second cylinder 8 (see FIG. 3A). In this state, the egg A stored in the carrier 26 is supplied to the positioning portion P on the mounting plate 2 located below the chorioallantoic membrane cutter 14 and the pressing member 16. The egg A has already been cut at the upper part of the eggshell G with the air chamber F facing upward in the previous step.
[0018]
Here, the operating rod 6 of the first cylinder 4 is contracted to lower the chorioallantoic membrane cutter 14 and the pressing member 16 to the lowest position (see FIG. 3B). When the chorioallantoic membrane cutter 14 and the pressing member 16 are integrally lowered, the distal end portion 16a of the pressing member 16 protruding downward from the cut portion 14a of the chorioallantoic membrane cutter 14 presses the chorioallantoic membrane E, and the upper part thereof. The cut portion 14a of the chorioallantoic membrane cutter 14 located at the position cuts the chorioallantoic membrane E.
[0019]
Next, the operating rod 10 of the second cylinder 8 is extended to slightly raise the chorioallantoic membrane cutter 14 and the pressing member 16 (see FIG. 3C). The chorioallantoic membrane cutter 14 was lowered greatly in order to break the chorioallantoic membrane E. However, at the most lowered position, the fetus D and the yolk C are strongly pressed and may be damaged. The chorioallantoic fluid cutter B is collected with the chorioallantoic membrane cutter 14 and the pressing member 16 slightly lifted.
[0020]
While the positions of the chorioallantoic membrane cutter 14 and the pressing member 16 with respect to the chicken egg A are in the state shown in FIG. 3C, the mounting plate 2 and the entire unit installed on the upper part thereof are rotated by a rotating means (not shown). Then, the rotation axis 20 is rotated. In this embodiment, it is rotated about 135 degrees (see FIG. 3D). When the egg A is tilted, the chorioallantoic fluid B is discharged through the space 22 between the chorioallantoic membrane cutter 14 and the pressing member 16 and the outer peripheral surface of the chorioallantoic membrane cutter 14 and is disposed below. It is recovered in the recovery toy 28 (see FIG. 3 (d)).
[0021]
After the chorioallantoic fluid B in the egg A is discharged and collected, the mounting plate 2 is reversed by the rotating means to return to the horizontal state (see FIG. 4A). At the time when the liquid collection is finished, the rod 6 of the first cylinder 4 is contracted and the rod 10 of the second cylinder 8 is in an expanded state. From this state, the first cylinder 4 is operated and the chorioallantoic membrane cutter 14 and the pressing member 16 is raised to the highest position (see FIG. 4B). Subsequently, the rod 10 of the second cylinder 8 is contracted to slightly lower the chorioallantoic membrane cutter 14 and the pressing member 16 to complete one cycle (see FIG. 4C). The egg A after the end of the collection is discharged, the next egg A is supplied, and the same operation is performed. In this way, the hen egg A that broke the chorioallantoic membrane E is tilted to collect the chorioallantoic fluid B, so that the chorioallantic fluid B present in the lower part of the hen's egg A can also be collected and efficient liquid collection can be performed. it can.
[0022]
Although FIG. 1 shows a chicken egg liquid collecting apparatus including a pair of chorioallantoic membrane cutters 14 and a pressing member 16, a tray in which a large number of eggs A are aligned vertically and horizontally is usually mounted on the mounting plate 2. And a plurality of sets of chorioallantoic membrane cutters 14 and pressing members 16 are provided in the same arrangement as that of the eggs A. In the above embodiment, the rising position and the lowering position of the chorioallantoic membrane cutter 14 and the pressing member 16 are each in two stages (see FIGS. 4B, 4C, 3B, and 3C). However, either one or both of the ascending position and the descending position may be one stage.
[0023]
Furthermore, although the rotation angle of the mounting plate 2 is set to 135 degrees, this can be rotated slowly because time can be saved as much as the rotation angle is small compared to the case of rotating 180 degrees. The liquid can be prevented from splashing with force. Further, since the load of the fetus D or the yolk C is not directly applied to the pressing member 16 and the chorioallantoic cutter 14, it is possible to prevent the fetus D and the like from being damaged or blocking the opening of the chorioallantoic cutter 14. In addition, when tilted at the angle as described above, there is an advantage that air can easily enter the egg A and the chorioallantoic fluid can be easily produced compared to the case where it is rotated 180 degrees to be vertical.
[0024]
FIG. 5 is a view showing an egg liquid collecting apparatus according to the second embodiment. In this embodiment, a mechanism for rotating the mounting plate 2 is not provided. Liquid B is aspirated and collected. As described above, the gap 22 is provided between the chorioallantoic membrane cutter 14 and the pressing member 16 therein, and the upper portion 14b of the chorioallantoic membrane cutter 14 is extended and sealed to make the inside as a nozzle. We are using. The same parts as those in the first embodiment are denoted by the same reference numerals, and the description thereof is omitted.
[0025]
In this embodiment, after the operations shown in FIGS. 3A to 3C, the chorioallantoic fluid B in the egg A is collected by being sucked by the vacuum pump 30 without rotating the mounting plate 2. In this configuration, since suction is performed while holding the fetus D and the like by the holding member 16, the lower chorioallantoic fluid B is pushed by the fetus D and the like and rises from the surroundings. A large amount of chorioallantoic fluid can be aspirated more efficiently than when only aspiration is performed.
[0026]
FIG. 6 is a view showing a chicken egg liquid collecting apparatus according to the third embodiment, and the liquid is collected by suction as in the second embodiment. In the second embodiment, the chorioallantoic membrane cutter is used. In this embodiment, the hose 40 separate from the chorioallantoic membrane cutter 14 is attached to the side of the chorioallantoic membrane cutter 14, and the hose 40 is used. The vacuum pump 30 is used for suction. Even in this configuration, the chorioallantoic fluid can be collected in the same process as in the second embodiment, and the same effect can be obtained. In the second embodiment, the gap 22 is necessary between the chorioallantoic membrane cutter 14 and the pressing member 16, but it goes without saying that the gap is not necessarily required in the case of the third embodiment. .
[0027]
In each of the above-described embodiments, the chorioallantoic membrane cutter 14 and the pressing member 16 inside the chorioallantoic membrane cutter 14 are lifted and lowered integrally, but may be lifted and lowered independently.
[0028]
【The invention's effect】
As described above, in the egg collection method according to the present invention, the chicken egg cut at the upper part of the eggshell with the air chamber side facing upward is positioned and placed above the egg, and the chorioallantoic membrane of the egg at the lower end The chorioallantoic membrane cutter in the chicken egg is lowered by lowering a chorioallantoic membrane cutter having a cut portion that breaks the upper surface and a pressing member provided inside the chorioallantoic membrane cutter and having a tip projecting downward from the cut portion. The chorioallantoic fluid in the hen's egg is collected while being broken and holding the fetus by the pressing member, so that the chorioallantoic fluid in the lower part of the hen's egg can also be collected and efficient liquid collection is possible. Moreover, it is possible to prevent egg yolk and the like from being mixed into chorioallantoic fluid.
[0029]
Moreover, in the egg collection device according to the present invention, the egg is placed above the egg on the placing plate, the placing plate for positioning and placing the egg, the rotating means for rotating the placing plate, and the lower end. A chorioallantoic membrane cutter having a cut portion that breaks the chorioallantoic membrane of the chicken egg, a pressing member provided inside the chorioallantoic membrane cutter and holding the fetus in the egg by a lower end protruding from the chorioallantoic membrane cutter, Elevating means for elevating and lowering the allantoic cutter and the pressing member, and a chorioallantoic fluid collection unit disposed below the mounting plate, and lowering the chorioallantoic membrane cutter and the pressing member and cutting the upper part So that the chorioallantoic membrane is broken and the chow plate is rotated by a predetermined angle while holding the fetus, and the chorioallantoic fluid in the egg is collected in the collecting part. So under the chicken egg Chorioallantoic fluid in the well can be recovered, it is possible to perform efficient solution sampling piece. Moreover, it is possible to prevent egg yolk and the like from being mixed into chorioallantoic fluid.
[Brief description of the drawings]
FIG. 1 is a front view showing a chicken egg liquid collecting apparatus according to an embodiment of the present invention.
FIG. 2 is an enlarged perspective view showing a lower end portion of the chorioallantoic membrane cutter and the pressing member of the embodiment.
FIG. 3 is a diagram sequentially illustrating each step of a method for collecting chicken eggs by the apparatus of the embodiment.
4 is a diagram showing a step following FIG. 3. FIG.
FIG. 5 is a front view showing a hen egg liquid collecting apparatus according to a second embodiment;
FIG. 6 is a front view showing a hen egg liquid collecting apparatus according to a third embodiment;
FIG. 7 is a cross-sectional view showing the inside of a growing chicken egg.
[Explanation of symbols]
A chicken egg D fetus E chorioallantoic membrane F air chamber G eggshell 2 placement plate 4 first cylinder (lifting means)
8 Second cylinder (lifting means)
14 Chorioallantoic membrane cutter 14a Cut part 16 Pressing member 16a Lower end part 28 of pressing member Collection part (collection toy)
30 suction means

Claims (5)

A chorioallantoic membrane cutter having a cut portion that positions the chicken egg cut at the upper part of the eggshell with the air chamber side facing upward and is disposed above the egg and that breaks the chorioallantoic membrane of the chicken egg, and the chorioallantoic membrane A pressing member provided inside the cutter and having a tip projecting downward from the cut part is lowered to break the chorioallantoic membrane in the egg and to remove the chorioallantoic fluid in the egg while holding the fetus by the pressing member. A method for collecting chicken eggs, comprising collecting the eggs. A chorioallantoic membrane cutter having a cut portion that positions the chicken egg cut at the upper part of the eggshell with the air chamber side facing upward and is disposed above the egg and that breaks the chorioallantoic membrane of the chicken egg, and the chorioallantoic membrane A pressing member provided inside the cutter and having a tip protruding downward from the cut portion is lowered to break the chorioallantoic membrane in the egg, and the egg is tilted by a predetermined angle while holding the fetus by the pressing member. A method for collecting hen's eggs, characterized by recovering the internal chorioallantoic fluid. A chorioallantoic membrane cutter having a cut portion that positions the chicken egg cut at the upper part of the eggshell with the air chamber side facing upward and is disposed above the egg and that breaks the chorioallantoic membrane of the chicken egg, and the chorioallantoic membrane The chorioallantoic cutter and the chorioallantoic cutter are provided inside the cutter by lowering a pressing member whose tip protrudes downward from the cut portion to break the chorioallantoic membrane in the egg and holding the fetus by the pressing member. A method for collecting hen's eggs, wherein the chorioallantoic fluid in the hen's egg is sucked and collected by a suction means provided in the vicinity of the pressing member. A placing plate for positioning and placing the egg, a rotating means for rotating the placing plate, and a cut plate disposed above the egg on the placing plate and having a cut portion that breaks the chorioallantoic membrane of the egg at the lower end. An allantoic cutter, a pressing member that is provided inside the chorioallantoic cutter and presses the fetus in the egg by a lower end protruding from the chorioallantoic cutter, and an elevating means that raises and lowers the chorioallantoic cutter and the pressing member; A chorioallantoic fluid recovery part disposed below the mounting plate,
The chorioallantoic membrane cutter and the pressing member are lowered, inserted into a chicken egg whose upper part is cut and placed on the mounting plate, and the mounting plate is rotated by a predetermined angle while breaking the chorioallantoic membrane and holding the fetus And collecting the chorioallantoic fluid in the hen egg in the collecting unit. A placing plate for positioning and placing the egg, a chorioallantoic cutter disposed above the egg on the placing plate and having a cut portion that breaks the chorioallantoic membrane at the lower end, and the inside of the chorioallantoic membrane cutter And a pressing member for holding the fetus in the egg by a lower end protruding from the chorioallantoic cutter, an elevating means for raising and lowering the chorioallantoic membrane cutter and the pressing member, and arranged in the vicinity of the chorioallantoic membrane cutter and the pressing member Suction means,
The chorioallantoic membrane cutter and the pressing member are lowered, inserted into a chicken egg whose upper part is cut and placed on the mounting plate, and the chorioallantoic membrane is broken and sucked by suction means while holding the fetus An egg collection device for collecting the chorioallantoic fluid.

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