JP4181353B2 - Skin reaction test member - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a skin reaction test member for testing a reaction of a skin or a human body to a test substance such as ethyl alcohol or allergen.
[0002]
[Prior art]
In general, various food components and chemical substances are known to cause allergic reactions in human skin. There are various types of allergens that cause allergies. Conventionally, allergen challenge tests have been conducted to examine the types of allergens and the strength of allergic reactions. Skin reaction tests may also be performed to evaluate the safety of various chemical substances.
[0003]
As this allergen load test, for example, there is a skin reaction test in which an allergen is brought into contact with the skin to observe erythema. Conventionally, in one patch test of a skin reaction test, a skin reaction test member is made by including a liquid containing a test substance in, for example, an aluminum fin chamber, filter paper or gauze. Here, the test substance is dissolved or dispersed in, for example, water or the like, soaked in filter paper or gauze to make a patch, and the patch is brought into contact with the skin through the fin chamber or as it is to form a skin reaction test member. Then, this skin reaction test member is fixed to the skin with an adhesive tape or the like, and after leaving for a predetermined time in a state where the test substance is in contact with the skin, the patch is peeled off, and the part of the skin that has been in contact with the test substance is observed, The degree of erythema that has reacted to the test substance is judged visually. That is, the degree of erythema on the reacted skin is used as a judgment material for skin reaction.
[0004]
Also, in the ethanol patch test for examining the constitution for alcohol, a patch soaked with ethanol was applied to the arm in the same manner as described above, and the metabolic capacity for alcohol was estimated from the redness of the skin in that portion.
[0005]
[Problems to be solved by the invention]
In the case of the above conventional technique, since the test substance of the skin reaction test member is a liquid, it is troublesome to handle, and the erythema of the skin reaction is the degree of sunburn of the subject, the color of the skin, the race and the genetic constitution. There were individual differences in the color of the skin, and it was difficult to accurately determine the presence or quantitativeness of erythema. For example, there is a problem that erythema is not visible to a person with a dark color, but erythema is noticeable even with a slight reaction to a person with a white color.
[0006]
Furthermore, the test using the skin reaction test member may have different test methods and determination criteria depending on conditions, facilities, and the like. For this reason, the determination on the reaction may be different. In addition, handling and test conditions for allergens and alcohol are strict and complicated, and determination may be difficult unless a specialized dermatologist. Furthermore, when the skin reaction is quantitatively tested, the amount of the test substance used in the skin reaction test member may be constant. For example, the amount of allergen used is as small as several mg, and quantitative weighing is difficult. There is a problem that it is difficult to judge accurately.
[0007]
The present invention has been made in view of the above-described conventional problems, and an object thereof is to provide a skin reaction test member capable of easily performing an accurate test.
[0008]
[Means for Solving the Problems]
The present invention is a skin reaction test member for inspecting the reaction of the skin surface in the human body, and is provided with a transparent color sample of the color of the skin that reacts with a test substance such as ethyl alcohol or allergen. A skin reaction test member which is capable of determining the degree of redness by overlaying the color sample on the skin which is not reddened adjacent to the skin.
[0009]
Furthermore, the present invention is provided with a gel body to which a test substance such as ethyl alcohol or allergen for inspecting the reaction of the skin surface in the human body, and an adhesive member provided with a pad portion soaked with the gel body, are provided on the pad portion. A skin reaction test member that soaks the gel body, affixes it to the skin surface of the human body, peels off after a certain period of time, and inspects the skin color of the part touched by the pad portion in comparison with the color sample. is there.
[0010]
Further, according to the present invention, a plurality of sheet-like gel bodies added with a test substance for inspecting the reaction of the skin surface in the human body are affixed on a release paper and packaged in a hermetically sealed state. It is a skin reaction test member that can be inspected by comparing the color of the skin that is attached to the surface and peeled off after a certain time and touched with the gel body in comparison with the color sample .
[0011]
Further, the present invention is such that a test liquid to which a test substance for inspecting the reaction of the skin surface in the human body is sealed and packaged, and a part of the container containing the test liquid faces a part such as a breakable aluminum foil. Providing a sticking member having a pad portion soaked with the test solution, breaking the container so as to soak the gel body into the pad portion, sticking the pad portion to the skin surface of the human body, and after a certain time It is a skin reaction test member which can test | inspect the color of the skin of the part which peeled and the said gel body touched compared with the said color sample .
[0012]
Furthermore, the present invention is such that a pressure-sensitive adhesive to which a test substance for inspecting the reaction of the skin surface in the human body is applied is applied to a sheet-like adhesive member, and the adhesive member is applied to the human skin surface and peeled off after a predetermined time. A skin reaction test member capable of inspecting a skin color of a part touched by the adhesive as compared with the color sample .
[0013]
The test substance is ethyl alcohol or an allergen that causes allergic reactions. Allergens are allergens such as pollen extract components, lacquer, eggs, fragrances, detergents, ginkgo, chrysanthemums, metals, pigments, hair dyes, preservatives, rubber, and other plants and drugs.
[0014]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, embodiments of the present invention will be described with reference to the drawings. 1 and 2 show a first embodiment of the present invention. In the skin reaction test member 10 of this embodiment, the adhesive 12 is provided at the center of the adhesive side surface of the adhesive member 14 provided on the inner surface. A pad 16 made of nonwoven fabric is provided. A large number of the sticking members 14 are continuously provided in a state where the pads 16 are provided, and can be appropriately torn off. Further, a plurality of sticking members 14 provided with pads 16 may be provided individually.
[0015]
In use, a gel 20 containing ethyl alcohol is applied to the pad 16. The gel 20 is composed of, for example, 44% ethyl alcohol, 54.5% purified water, 0.5% 1,3 butylene glycol, 0.5% xanthan gum, 0.5% CMCNa, and is contained in the aluminum laminate tube 22, Squeeze and use the required amount.
[0016]
In the method of using the skin reaction test member 10 of this embodiment, the gel 20, which is a gel body to which ethyl alcohol, which is a test substance for examining the reaction in the human body, is squeezed out from the tube 22 and applied to the pad 16. Then, the gel 20 is soaked into the pad portion 16 and applied to the skin surface of the human body, for example, the inside of the arm 26, which is less affected by sunburn or the like and easily causes a skin reaction. Then, after a certain period of time, for example, after a few minutes, the skin color of the part peeled and touched by the pad portion 16 is inspected.
[0017]
The gel 20 may be one to which various allergens are added in addition to ethyl alcohol which is a test substance. Examples of the allergen include pollen extract, egg, fragrance, detergent, lacquer, ginkgo, chrysanthemum, pigment, hair dye, preservative, rubber, other plants, various drugs and metals. When the test substance is water-soluble, it is dissolved in purified water and added. The oil-soluble component is added after being emulsified with a surfactant. The insoluble material is powdered and dispersed in the gel. The addition amount with respect to a gel can be set arbitrarily. The allergen concentration is 0.05 to 30%, preferably 1 to 10%, and is appropriately set depending on the type of allergen.
[0018]
For example, as a gel containing allergen, a predetermined allergen is mixed to a required concentration in a gel composed of 98% purified water, 1.0% xanthan gum, 0.5% CMCNa, and 0.5% 1,3 butylene glycol. . In the same manner as described above, the method of use is housed in an aluminum laminate tube, and the required amount is squeezed out.
[0019]
The inspection method uses a judgment sheet 32 which is a skin reaction test member in which a color sample 30 for coloring the skin that reacts with ethyl alcohol is provided with transparency. In the determination, the color sample 30 is placed on the skin that is not reddened adjacent to the skin of the reddish part of the arm 26, and the transparent part 34 of the determination sheet 32 is positioned on the reddish part to see the degree of redness of the red spot. In the case of the test for ethyl alcohol, the level judgment is performed in 3 to 4 stages from no redness to the most red ++. Moreover, in the case of the test | inspection with respect to an allergen, it can set with the kind of allergen from 2 steps to about 10 steps suitably.
[0020]
According to this embodiment, a large amount of determination can be easily performed at low cost. Furthermore, since the test substance such as ethyl alcohol is not the liquid but the gel 20, it can be accommodated in the tube 22 and easily applied onto the pad 16, and is easy to handle. Further, by using the determination sheet 32 provided with the transparent color sample 30, accurate determination can be performed regardless of the color of the skin.
[0021]
Next, a second embodiment of the present invention will be described. The skin reaction test member 40 of this embodiment is integrally laminated on a thin support 41 formed in a cross shape, a square shape, or a circle shape as shown in FIGS. 4 (A), (B), (C). It consists of a gel body 42. The gel body 42 is, for example, purified water 59%, glycerin 18.35%, sodium polyacrylate 8%, polyacrylic acid 2%, CMCNa 2%, tartaric acid 0.6%, dry aluminum hydroxide gel 0.05%, ethyl A solution in which 10% of alcohol is mixed is gelled. When gelling, this solution is applied at a rate of 70 mg / cm ² to a support 41 made of a polypropylene film with a thickness of 25 μm, to which a non-woven fabric made of polypropylene having a basis weight of 20 g / m ² is bonded, and gelled. . The gelled surface is covered with a separator 43 which is a release paper such as a polypropylene film having a thickness of 30 μm. At the time of use, one gel body 42 is peeled off from the separator 43 and attached to the skin.
[0022]
It should be noted that various allergens can be added to the gel body 42 as test substances instead of ethyl alcohol. Allergen types are as described above.
[0023]
The skin reaction test member 40 is used in the same manner as in the above-described embodiment, in which the skin reaction test member 40 is attached to an arm or the like and peeled off after a predetermined time, and the degree of redness of the portion is determined. The determination method is the same as in the above embodiment. Moreover, you may determine by observing directly, without using a color sample.
[0024]
In addition, as shown in FIG. 5, an adhesive member 46 such as a film or a sheet coated with the adhesive 44 may be provided separately and attached to the skin together with the skin reaction test member 40. Thereby, the skin reaction test member 40 is hard to come off, and the inspection becomes easy.
[0025]
Next, a third embodiment of the present invention will be described. As shown in FIGS. 6 and 7, the skin reaction test member 50 of this embodiment includes a PTP package in a plastic container 54 in which a test liquid 52 to which a test substance for inspecting a reaction in a human body is added is blister packed. The aluminum foil 56 side of the container 54 in which the test solution 52 is accommodated is formed so as to be breakable. On the aluminum foil 56 side, a sticking member 60 provided with a pad portion 58 soaked with the test solution 52 is provided, and the sticking member 60 adheres to the aluminum foil 56 with an adhesive 62.
[0026]
The usage method is to push the container 54 with a finger to break the aluminum foil 56 and soak the test liquid 52 into the pad portion 58. Thereafter, the pad portion 58 is applied to the skin surface of the human body, and the reaction in the human body is examined.
[0027]
The skin reaction test member 50 is also used in the same manner as in the above embodiment. The skin reaction test member 50 is affixed to an arm or the like and peeled off after a predetermined time to determine the degree of redness of the portion. The determination method is the same as in the above embodiment.
[0028]
Next, a fourth embodiment of the present invention will be described with reference to FIG. The skin reaction test member 66 of this embodiment is provided with a sticking member 64 such as a film or sheet to which an adhesive 62 is applied, and a test substance is added to the adhesive 62. The test substance is the same as in the above embodiment. The pressure-sensitive adhesive 62 can be appropriately selected from acrylic, SIS, silicon, natural rubber, and the like. A separator (not shown) is attached to the adhesive 62 and is peeled off during use.
[0029]
Note that the test member of the present invention is not limited to the above embodiment, and the gel body may be peeled off from the separator without being provided with a support and may be directly applied to the skin. In addition, the test substance can be selected as appropriate, and any substance can be used as long as the reaction by the human skin appears.
[0030]
【The invention's effect】
According to the skin reaction test member of the present invention, it is easy to handle and can be easily tested at low cost. Then, it is possible to accurately determine the degree of erythema and examine the reaction to allergen, alcohol and the like.
[Brief description of the drawings]
FIG. 1 is a perspective view showing a skin reaction test member according to a first embodiment of the present invention.
FIG. 2 is a perspective view showing how to use the skin reaction test member of the first embodiment of the present invention.
FIG. 3 is a plan view showing a determination sheet for a skin reaction test member according to the first embodiment of the present invention.
FIG. 4 is a plan view of a skin reaction test member according to a second embodiment of the present invention.
FIG. 5 is a perspective view of another example of the skin reaction test member according to the second embodiment of the present invention.
FIG. 6 is a perspective view showing a skin reaction test member according to a third embodiment of the present invention.
FIG. 7 is a cross-sectional view of a skin reaction test member according to a third embodiment of the present invention.
FIG. 8 is a plan view showing a skin reaction test member according to a fourth embodiment of the present invention.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 10 Skin reaction test member 12 Adhesive 14 Sticking member 16 Pad 20 Gel 42 Gel body

Claims (6)

In a skin reaction test member that examines the reaction of the skin surface in the human body, a color sample of the color of the skin that reacts with the test substance is provided with transparency, on the skin that is not reddened adjacent to the red skin. A skin reaction test member characterized in that the color sample can be overlapped to determine the degree of redness . A sheet-like gel body to which a test substance for inspecting the reaction of the skin surface in the human body is added is affixed on a release paper , packaged in a sealed state, the gel body is affixed to the human skin surface, and is peeled off after a certain period of time. the skin color of a portion the gel body was touched, skin reaction test member according to claim 1, characterized in that a possible inspection as compared with the color sample Te. A test solution to which a test substance for inspecting the reaction of the skin surface in the human body is sealed and packaged, and a pad part that is allowed to soak into the test solution facing a breakable portion of a part of the container containing the test solution is provided. An adhesive member provided was provided, the container was broken and the pad portion was soaked with the test solution , the pad portion was applied to the skin surface of the human body, and the test solution was peeled off after a certain period of time. portion of the skin color, skin reaction test member according to claim 1, characterized in that inspectable and compared with the color sample. A pressure-sensitive adhesive added with a test substance for examining the reaction of the skin surface in the human body is applied to a sheet-like sticking member, the sticking member is stuck on the human skin surface, peeled off after a certain time, and touched by the pressure-sensitive adhesive. the color of the skin of which was part, skin reactions test member according to claim 1, characterized in that a possible inspection as compared with the color sample.     The skin reaction test member according to claim 1, 2, 3 or 4, wherein the test substance is ethyl alcohol.     The skin reaction test member according to claim 1, 2, 3, or 4, wherein the test substance is an allergen.

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