JP4157494B2 - 植物における環境ストレス耐性能力の評価法 - Google Patents
植物における環境ストレス耐性能力の評価法 Download PDFInfo
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- JP4157494B2 JP4157494B2 JP2004144067A JP2004144067A JP4157494B2 JP 4157494 B2 JP4157494 B2 JP 4157494B2 JP 2004144067 A JP2004144067 A JP 2004144067A JP 2004144067 A JP2004144067 A JP 2004144067A JP 4157494 B2 JP4157494 B2 JP 4157494B2
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Description
別の実施態様において、光合成の光阻害は、クロロフィル蛍光の測定によって定量される。
別の実施態様において、飽和光パルスは、500μmol photon m-2s-1〜30,000μmol photon m-2s-1の光量子束密度である。
別の実施態様において、飽和光パルスの照射持続時間は、10μ秒〜10秒である。
別の実施態様において、ある飽和光パルスの照射開始と次の飽和光パルスの照射開始との間の照射間隔が、15秒〜30分である。
別の実施態様において、飽和光パルスの最初の照射開始から最後の照射終了までの時間が少なくとも2時間である。
別の実施態様において、光合成の光阻害は、室温又は環境温度で測定される。
6CO2 + 12H2O → C6H12O6+ 6O2 + 6H2O
により、水の酸化により生成した電子を用いて二酸化炭素を還元し、炭水化物を合成する。光合成は大別して二つの段階からなる。共に細胞内小器官である葉緑体で行われるが、第一段階は葉緑体内のチラコイド膜で、第二段階は葉緑体内の液状基質のストロマで行われる。第一段階は一般的には明反応と呼ばれ光照射に依存する反応であり、第二段階は暗反応と呼ばれ光に依存しない反応である。
光化学系II量子収率 =(Fm-F0)/Fm (I)
(ここで、Fm及びF0は上記の意味を有する。)から求めることができる。光阻害を受けていない状態にある植物の光化学系II量子収率は0.75〜0.85の範囲内にあることが知られている。また、光阻害の程度を反映して光化学系II量子収率は低下する(図1参照)。
Claims (10)
- 光合成作用光の非存在下、植物の光合成器官に飽和光パルスを間欠照射し、これによって引き起こされる光合成の光阻害のレベルを測定して植物の光ストレス耐性を評価することを含む、植物の環境ストレス耐性能力を評価する方法。
- 光ストレス耐性が葉緑体における活性酸素消去の能力に依存する、請求項1に記載の方法。
- 光合成の光阻害がクロロフィル蛍光の測定によって定量される、請求項1又は2に記載の方法。
- 飽和光パルスが500μmol photon m-2s-1〜30,000μmol photon m-2s-1の光量子束密度である、請求項1〜3のいずれか1項に記載の方法。
- 飽和光パルスの照射持続時間が10μ秒〜10秒である、請求項1〜4のいずれか1項に記載の方法。
- ある飽和光パルスの照射開始と次の飽和光パルスの照射開始との間の照射間隔が、15秒〜30分である、請求項1〜5のいずれか1項に記載の方法。
- 飽和光パルスの最初の照射開始から最後の照射終了までの時間が少なくとも2時間である、請求項1〜6のいずれか1項に記載の方法。
- 光合成の光阻害が室温又は環境温度で測定される、請求項1〜7のいずれか1項に記載の方法。
- 活性酸素分解酵素で形質転換された植物を準備し、請求項1に記載の方法によって該植物の光阻害のレベルを測定し、野生型植物と比較して光阻害が小さい、したがって環境ストレス耐性能力を有する、植物を同定するための方法。
- 植物の環境ストレス耐性能力を評価するための装置であって、飽和光パルスの光質を変えるための手段を含むクロロフィル蛍光測定装置において、飽和光パルスを間欠照射するための条件設定を可能にする手段をさらに含むことを特徴とする、上記装置。
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JP4157494B2 true JP4157494B2 (ja) | 2008-10-01 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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KR102413052B1 (ko) * | 2021-10-26 | 2022-06-24 | 한경대학교 산학협력단 | 단말기에서 식물의 서식지 결정 계수를 처리하는 장치 및 방법 |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1804589B (zh) * | 2005-12-28 | 2010-05-12 | 华南师范大学 | 一种植物逆境生理监测与高抗植物筛选的方法及其装置 |
EP2217695A2 (en) * | 2007-11-10 | 2010-08-18 | Joule Unlimited, Inc. | Hyperphotosynthetic organisms |
CN102015995B (zh) | 2008-03-03 | 2014-10-22 | 焦耳无限科技公司 | 产生碳基目的产物的二氧化碳固定工程微生物 |
JP6485850B2 (ja) | 2013-05-17 | 2019-03-20 | 国立大学法人京都大学 | 植物の活力診断方法、並びにこれに用いられる計測システム及び診断システム |
JP6470987B2 (ja) * | 2015-01-28 | 2019-02-13 | 日本製紙株式会社 | 発根に適した光強度を評価する方法 |
EP4245125A1 (en) * | 2020-11-12 | 2023-09-20 | National University Corporation Kobe University | Diagnosis device for environmental stress in plants and environmental stress diagnosis method |
US20240019372A1 (en) * | 2020-11-12 | 2024-01-18 | Kyoto University | Device for diagnosing environmental stress in plants and method for diagnosing environmental stress |
KR102399776B1 (ko) * | 2020-11-25 | 2022-05-23 | 전북대학교산학협력단 | 상추 유전자원의 내염성 평가 지표 산출 방법 |
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KR102413052B1 (ko) * | 2021-10-26 | 2022-06-24 | 한경대학교 산학협력단 | 단말기에서 식물의 서식지 결정 계수를 처리하는 장치 및 방법 |
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