JP4154458B2 - Strawberry culture method - Google Patents

Strawberry culture method Download PDF

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JP4154458B2
JP4154458B2 JP01999399A JP1999399A JP4154458B2 JP 4154458 B2 JP4154458 B2 JP 4154458B2 JP 01999399 A JP01999399 A JP 01999399A JP 1999399 A JP1999399 A JP 1999399A JP 4154458 B2 JP4154458 B2 JP 4154458B2
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strawberry
seedlings
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liquid medium
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JP2000217456A (en
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雄一 米津
智一 北野
文明 小田
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MIKADO KYOWA SEED CO. LTD.
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MIKADO KYOWA SEED CO. LTD.
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Description

【0001】
【発明の属する技術分野】
本発明は、イチゴの苗を大量培養する技術に関する。
【0002】
【従来の技術】
従来、イチゴ用液体培地組成物としては、MS培地にショ糖を含有させてあるものが知られており、さらに、そのイチゴ用液体培地組成物にベンジルアデニン(以下BAと略称する)を含有させてあるものも知られており、そのようなイチゴ用液体培地組成物のみによってイチゴの芽数を増やし(増殖)、かつ、草丈を伸ばし(伸長)、かつ、赤みがかった太いものに肥大し、自然環境の乾燥等に耐えやすい組織を形成することが行われていた。
【0003】
【発明が解決しようとする課題】
上述した従来のイチゴの栽培方法によれば、イチゴの苗は増殖し、かつ、伸長するものの、その増殖度合いや、伸長度合いには不十分な面があり、増殖効率が低かったり、十分な草丈にならず、取り扱い困難な苗しか得られないという状況があった。また、それに加えて、十分自然環境の乾燥等に耐える組織を形成させにくく、自然環境に順化させたとしても、十分丈夫な苗に生育しにくいという問題点も指摘されており、より確実に栽培容易なイチゴの苗を得る技術が望まれていた。
【0004】
従って、本発明の目的は、上記実情に鑑みなされたものである。
【0005】
【課題を解決するための手段】
本発明者らは、液体培地の組成を変更することにより、栽培容易なイチゴの苗を得ることができるようになるのではないかと考え、種々の液体培地組成物について調べたところ、イチゴの芽数の増殖度、草丈の伸長度、苗の肥大度に関する種々の新知見を得た。本発明は、前記新知見に基づき成されたものであって、
この目的を達成するための本発明の特徴手段は、イチゴの苗の芽の数を増やす増殖期に、2〜10倍希釈MS液体培地に0.5%以上2%未満のブドウ糖、及び、0.5mg/リットル以下のベンジルアデニン(BA)を含ませてある培地で前記イチゴの苗を培養し、
前記増殖したイチゴの苗を伸長させる伸長期に、窒素、燐、カリウムの比(N:P:K)が、6.5:6:19である肥料に、MS培地の微量要素とMS培地のビタミン類要素、及び、30mg/リットルのエチレンジアミン四酢酸鉄、及び、0.5%〜3%のブドウ糖、及び、0.5mg/リットル以下のベンジルアデニン(BA)を含有させてある液体培地で前記イチゴの苗を培養し、
その後、前記伸長したイチゴの被培養組織を地植え出来るように成長させる肥大期に、〜10倍希釈MS培地に、2%以上5%以下のブドウ糖もしくはショ糖、及び、0.5mg/リットル以下のベンジルアデニン(BA)を含有させてある液体培地で前記イチゴの苗培養することにあり、その作用・効果は以下の通りである。
【0006】
〔作用効果〕
まず、本発明者らは、液体培地によってイチゴの苗を増殖させる場合には先述のMS培地を希釈して用いると、イチゴの苗の増殖効率を高くする事ができるという知見を得ている(特願平5−158429号公報参照)。これに基づき、さらに、前記液体培地の組成を種々に変更を加えたところ、糖の含有量を比較的低濃度に抑制しておけば、前記イチゴの苗の増殖率を高くできるという新知見を得た。さらに本発明者らは、多糖類よりも単糖類の方がイチゴ苗による吸収性が良いと考え、種々の液体培地について調べたところ、糖としては、ショ糖を用いるよりも、ブドウ糖を用いた場合には、抑えるより高い増殖効率が得られるという新知見を得るに至った。さらに、本発明者らは、ブドウ糖の含有率について検討を加えたところ、0.5%以上2%未満としておくことが望ましいという知見を得た。
また、窒素、燐、カリウムの比(N:P:K)が、6.5:6:19である肥料に、MS培地の微量要素とMS培地のビタミン類要素、及び、30mg/リットルのエチレンジアミン四酢酸鉄を含有させておいた培地を用いた場合に、イチゴの草丈が極めて効率よく伸長するという新知見を得た。また、イチゴ用液体培地組成物には、BA及び糖を含有させておくことが望ましい。
尚、この場合糖としてはショ糖よりもブドウ糖を用いる方が望ましいという新知見も得ている。
またさらに、MS培地の4倍希釈液に2%以上5%以下の糖を添加してあるばあいに、特に、イチゴの苗が、赤みがかった太いものになり(肥大し)やすくなり、自然環境の乾燥等に耐えやすい組織を形成しやすいという新知見を得た。
また、この場合も植物を増殖させる培地組成物に、BAを添加しておくと、植物の増殖が効率よく行われるということも知られている。
つまり、イチゴの苗を大量培養するに際しては、増殖、伸長、肥大の3要素が揃ったときに、自然環境下で確実に栽培容易なイチゴの苗を効率良く提供できるわけであり、これらの要素を一種の培地組成物のみで満たそうとする点に従来の培養方法の無理があったと考え、これらの3要素を満たすために、それぞれの要素を満たすのに適した培地組成物を用いて培養を行えば、前述の3要素を全て満たして自然環境下で確実に栽培容易なイチゴの苗を提供できるということが言えるわけである。
そこで、上述の種々の新知見より、イチゴの苗を増殖させるための増殖期に、2〜10倍希釈MS液体培地に0.5%以上2%未満のブドウ糖、及び、0.5mg/リットル以下のベンジルアデニン(BA)を含ませてある培地で、そのイチゴ苗を増殖させ、さらに、窒素、燐、カリウムの比(N:P:K)が、6.5:6:19である肥料に、MS培地の微量要素とMS培地のビタミン類要素、及び、30mg/リットルのエチレンジアミン四酢酸鉄、及び、0.5%以上3%以下の糖、及び、0.5mg/リットル以下のベンジルアデニン(BA)を含有させておいた培地で増殖したイチゴの苗を伸長させ、さらに、MS培地の2〜4倍希釈液に2%以上5%以下の糖、及び、0.5mg/リットル以下のベンジルアデニン(BA)を添加してある培地で肥大させれば、イチゴの苗は、増殖し、かつ、伸長した丈夫な苗(以下健苗と称する)に成長し、自然環境に移植した場合にも、確実に作物を栽培できるイチゴの苗を効率よく提供できるようになった。
【0007】
【発明の実施の形態】
以下に本発明の実施の形態を図面に基づいて説明する。
まず、常法に従ってイチゴの茎の先端の生長点を得るとともに、常法にしたがって培養し、1つの植物体1(以下、この段階の植物体を苗と称する)にする。この苗を、ステンレス鋼製の網2aからなる支持台2の上に支持させ、密封容器3に設けた載置部3aに載置する。また、密封容器3中には植物体の下部に接するように液体培地4として培地A(後述)が入れてある。そして、この密封容器は、振とう台上に固定して培養期間中振とうを続け、前記培地Aを常時攪拌して、クラウン部に栄養分を供給し続けるようにして、収容した植物体を5週間培養して増殖させる。(図1参照)
【0008】
このようにしてイチゴの苗を増殖させると、平均約130倍に増加した。さらに増殖したイチゴ苗は、株分けし、クラウン部の上下各2cmの所で切断し、クラウン部1dを含まない根部1a、葉部1b、茎部1cを除去した後、このクラウン部を含む切断されたイチゴ苗を再生した植物体1としてさらに培養、増殖される。
【0009】
増殖させた前記苗の3本を、クラウン部の上下各2cmの所で切断し、クラウン部1dを含まない根部1a、葉部1b、茎部1cを除去した後、先と同様に、液体培地4とともに密封容器3中に入れ、振とう台上に固定して培養期間中振とうを続けながら、前記収容した苗を9週間培養して増殖伸長させる。このとき、液体培地4としては培地Bを用いた。
【0010】
このようにしてイチゴの苗を培養させると、草丈15mm以上のもの(健苗)が容器当たり平均約130本に増加するとともに、ほぼ全般的に2cm以上の取り扱い容易な長さに伸長した。
【0011】
次に、伸長した前記苗を、前記液体培地4とともに密封容器3中に収容してに固定して培養期間中振とうを続け、前記液体培地4を常時攪拌して、クラウン部に栄養分を供給し続けるようにして、収容した植物体を5週間培養して増殖させる。このとき、液体培地4としては培地Cを用いた。
【0012】
このようにしてイチゴの苗を増殖させると、赤みがかった丈夫な苗になっていることが判った。さらに肥大したイチゴ苗は、株分けし培土に移植された後、イチゴの果実を栽培するため順化して、自然環境下に移植して用いられる。
【0013】
尚、ここで用いた振とう台は、振とう台上に固定した密封容器3を、水平面上に円を描くように垂直軸心回りに回転させ、密封容器3内の培地4を攪拌出来るものである。また、ここで用いる密封容器とは、内外で気体の出入りを許容し、かつ、雑菌等の侵入を遮断する容器を意味する。
【0014】
尚、液体培地AとしてはMS培地(後述)を、4倍に希釈したもの(以下1/4MSと称す)(以下1/nMSと称するものはMS培地をn倍に希釈したものを指す)にブドウ糖を、1重量%、BAを、0.03mg/リットルになるように添加してある培地である。前記液体培地Bには、窒素、燐、カリウムの比(N:P:K)が、6.5:6:19である液体肥料として、微粉ハイポネックス(ハイポネクス社製)の0.1%水溶液にMS培地の微量要素(後述)とMS培地のビタミン類要素(後述)とを含有させてあるものを基本培地(以下HY培地と称する)とし、0.5%以上3%以下のブドウ糖、及び、30mg/リットルのエチレンジアミン四酢酸鉄、及び、0.5mg/リットル以下のベンジルアデニン(BA)を含有させてあるものを用いた。また液体培地Cとしては1/4MS培地にブドウ糖を4重量%、BAを、0.03mg/リットルになるように添加してある培地を用いた。
【0015】
以下にMS培地の組成、及び、MS培地の微量要素、ビタミン類要素の組成を1リットル当たりの重量(単位mg)で示す。
【0016】

Figure 0004154458
尚、エチレンジアミン四酢酸鉄を含有させておく場合には、硫酸鉄7水和物、及び、エチレンジアミン4酢酸2ナトリウムは省略する。
【0017】
Figure 0004154458
【0018】
MS培地のビタミン類要素:
ニコチン酸 …………………… 0.5
チアミン塩酸塩 …………………… 0.1
ピリドキシン塩酸塩 …………………… 0.5
m−イノシト−ル …………………… 100
グリシン …………………… 2
【0019】
【実施例】
以下に本発明の実施例を図面に基づいて説明する。
常法に従って生長点を培養して得たイチゴの苗1を3本づつ、培地A1、A2,D1〜D4の50mlずつ入った密封容器3それぞれに密封し、前記振とう台を用いて液体培地4を常時攪拌し続ける条件下、35日間イチゴ苗の培養を行って苗の増殖数を調べた。
尚、各培地の組成は、表1に示す通りである。
その結果表2のようになった。
【0020】
【表1】
Figure 0004154458
【0021】
【表2】
Figure 0004154458
【0022】
つまり、先の液体培地組成物A1でイチゴ苗1を培養して増殖を行うと、実験誤差によるばらつきがあるものの、130倍程度増殖することが分かり、糖としてブドウ糖を用いた場合にはショ糖を用いた場合に比べ高い増殖性を示すことがわかった。
また、1/4MSに、カイネチンを添加した液体培地4(培地D2〜D5)を用いた場合には、BAを用いた場合に比べ、増殖速度が全体的に低いものの、BAを用いた場合に匹敵する増殖率を示した。つまり、1/2MS〜1/10MSにカイネチンを添加した液体培地4を用いれば、高い増殖率を得られ、この培地にBAを添加すれば、試験例1以上の増殖率の向上を期待することが出来る。尚、カイネチンは、1mg/リットル以下の範囲で用いていればBAと同様の効果を奏することが経験的に知られている。
【0023】
上述の方法によって増殖したイチゴの苗1を3本づつ、培地B1、B2,D6,D7の50mlずつ入った密封容器3それぞれに密封し、前記振とう台を用いて液体培地4を常時攪拌し続ける条件下、培養を行ってイチゴ苗の15mm以上のものの数を調べた。
尚、各培地は液体培地A1の基本培地の種類や濃度を様々に変更したものであって、表3に示す通りである。
【0024】
その結果、平均増殖本数は表4のようになった。
尚、培養期間は9週間である。
【0025】
【表3】
Figure 0004154458
【0026】
【表4】
Figure 0004154458
【0027】
〔試験例2〕
表3に示す液体培地D及び液体培地Eを用いて、試験例1と同様にイチゴ苗を6週間培養して、15mm以上の苗の数を調べた。その結果表4のようになった。
【0028】
表4より、HY培地を用いた場合には、25mm〜35mmに伸長した比較的長い芽の割合が高く、かつ、取り扱い容易な長い芽数も多く培養されていることがわかる。また同様に、表5に示す液体培地B2及びB3を用いて、イチゴ苗を6週間培養して15mm以上の苗の数を調べた。その結果表6のようになった。
【0029】
【表5】
Figure 0004154458
【0030】
【表6】
Figure 0004154458
【0031】
その結果、伸長期に用いる液体培地組成物に用いる糖としては、ショ糖に比べてブドウ糖が好ましいことがわかった。
【0032】
次に、先の培養によって伸長したイチゴの苗1を、培地C1〜C5のそれぞれ50mlとともに各別に密封容器3に密封し、前記振とう台を用いて液体培地4を常時攪拌し続ける条件下、培養を行ってイチゴ苗の草丈が15mm以上で、かつ、赤みがかった太いもの肥大したものの数を調べた。尚、各培地の組成は、表3に示す通りである。
【0033】
その結果、平均増殖本数は図のようになった。
【0034】
【表7】
Figure 0004154458
【0035】
図2より、ショ糖濃度の高い1/4MS培地を用いた場合には、25mm〜35mmで肥大した芽の割合が高く培養されていることがわかる。
また、各培地C6〜C8のそれぞれ50mlとともに各別に密封容器に封入し、同様に培養したところ、図3のようになった。図3より、培地に含有させる糖分はショ糖よりもブドウ糖が好ましく、糖濃度としては3〜5%程度が好ましいということがわかる。
【図面の簡単な説明】
【図1】イチゴ培養方法を示す工程図
【図2】イチゴ肥大度のショ糖濃度依存性を示すグラフ
【図3】イチゴ肥大度の糖度依存性を示すグラフ[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a technology for mass-culturing strawberry seedlings.
[0002]
[Prior art]
Conventionally, liquid medium compositions for strawberries are known in which MS medium contains sucrose, and the liquid medium composition for strawberries contains benzyladenine (hereinafter abbreviated as BA). The number of strawberry buds is increased (growth), the plant height is increased (elongation), and it is enlarged to a reddish and thicker one using only the liquid medium composition for strawberry. Formation of a structure that can easily withstand the drying of the environment has been performed.
[0003]
[Problems to be solved by the invention]
According to the above-mentioned conventional strawberry cultivation method, the strawberry seedling grows and grows, but there is an insufficient aspect in the degree of growth and the degree of growth, the propagation efficiency is low, and the plant height is sufficient. There were situations where only seedlings that were difficult to handle were obtained. In addition, it has been pointed out that it is difficult to form a tissue that can withstand the drying of the natural environment, and even if it is adapted to the natural environment, it is difficult to grow into a sufficiently strong seedling. A technique for obtaining easy-to-cultivate strawberry seedlings has been desired.
[0004]
Therefore, the object of the present invention has been made in view of the above circumstances.
[0005]
[Means for Solving the Problems]
The present inventors considered that by changing the composition of the liquid medium, it would be possible to obtain strawberry seedlings that can be easily cultivated, and examined various liquid medium compositions. Various new findings were obtained regarding the number of growth, plant height elongation and seedling hypertrophy. The present invention has been made based on the above new findings,
In order to achieve this object, the characteristic means of the present invention includes 0.5% or more and less than 2% glucose in a 2 to 10-fold diluted MS liquid medium in the growth phase to increase the number of strawberry seedling buds, and 0 Cultivating the strawberry seedling in a medium containing not more than 5 mg / liter of benzyladenine (BA),
In the extension period for extending the grown strawberry seedlings, fertilizers with a ratio of nitrogen, phosphorus, and potassium (N: P: K) of 6.5: 6: 19 were added to the trace elements of the MS medium and the MS medium. In a liquid medium containing a vitamin component, 30 mg / liter of iron ethylenediaminetetraacetate, 0.5% to 3% glucose, and 0.5 mg / liter or less of benzyladenine (BA) Cultivate strawberry seedlings,
Thereafter, in the hypertrophy stage where the cultured tissue of the stretched strawberry is grown so that it can be planted in the ground, 2% to 5% glucose or sucrose, and 0.5 mg / liter or less to a 10-fold diluted MS medium The strawberry seedling is cultured in a liquid medium containing benzyladenine (BA), and its actions and effects are as follows.
[0006]
[Function and effect]
First, the present inventors have obtained the knowledge that when a strawberry seedling is grown in a liquid medium, the above-described MS medium can be used by diluting it to increase the growth efficiency of the strawberry seedling ( (See Japanese Patent Application No. 5-158429). Based on this, when the composition of the liquid medium was changed variously, the new finding that the growth rate of the strawberry seedling can be increased if the sugar content is suppressed to a relatively low concentration. Obtained. Furthermore, the present inventors considered that monosaccharides are better absorbed by strawberry seedlings than polysaccharides, and examined various liquid media. As sugar, glucose was used rather than sucrose. In some cases, new findings have been obtained that a higher proliferation efficiency can be obtained. Furthermore, the present inventors have examined the glucose content, and have found that it is desirable to set the content to 0.5% or more and less than 2%.
In addition, a fertilizer having a ratio of nitrogen, phosphorus and potassium (N: P: K) of 6.5: 6: 19, a trace element of MS medium, a vitamin element of MS medium, and ethylenediamine of 30 mg / liter When using a medium containing iron tetraacetate, a new finding was obtained that the plant height of strawberries grew extremely efficiently. Moreover, it is desirable that the liquid medium composition for strawberries contains BA and sugar.
In this case, there is a new finding that it is preferable to use glucose as the sugar rather than sucrose.
Furthermore, when 2% or more and 5% or less of sugar is added to a 4-fold dilution of MS medium, especially strawberry seedlings tend to become reddish and thick (hypertrophies), and the natural environment We obtained new knowledge that it is easy to form a tissue that can withstand the drying of the skin.
In this case, it is also known that if BA is added to the medium composition for growing the plant, the plant is efficiently propagated.
In other words, when mass-cultivating strawberry seedlings, it is possible to efficiently provide strawberry seedlings that are easy to cultivate reliably in the natural environment when the three elements of growth, elongation and hypertrophy are available. In order to satisfy these three elements, culture using a medium composition suitable for satisfying each of these elements is considered to have been impossible with conventional culture methods in that it is intended to satisfy only one kind of medium composition. In other words, it can be said that strawberry seedlings that satisfy all the above-mentioned three elements and can be easily cultivated in a natural environment can be provided.
Therefore, from the various new findings described above, in the growth phase for growing strawberry seedlings, 0.5 to 2% glucose and 0.5 mg / liter or less in a 2 to 10-fold diluted MS liquid medium The strawberry seedlings were grown in a medium containing benzyladenine (BA), and fertilizers having a ratio of nitrogen, phosphorus and potassium (N: P: K) of 6.5: 6: 19 , MS medium medium element and MS medium vitamin element, 30 mg / liter iron ethylenediaminetetraacetate, 0.5% to 3% sugar, and 0.5 mg / liter benzyladenine ( Strawberry seedlings grown in a medium containing BA) are elongated, and 2 to 4 times dilution of MS medium with 2% to 5% sugar and 0.5 mg / liter or less benzyl With adenine (BA) If grown in a certain medium, the strawberry seedling grows and grows into a strong and strong seedling (hereinafter referred to as “healthy seedling”). Strawberry seedlings can be efficiently provided.
[0007]
DETAILED DESCRIPTION OF THE INVENTION
Embodiments of the present invention will be described below with reference to the drawings.
First, a growth point at the tip of a strawberry stalk is obtained according to a conventional method, and cultured according to a conventional method to form one plant body 1 (hereinafter, the plant body at this stage is referred to as a seedling). The seedling is supported on a support base 2 made of a stainless steel net 2 a and placed on a placement portion 3 a provided in the sealed container 3. In addition, a medium A (described later) is placed as a liquid medium 4 in the sealed container 3 so as to be in contact with the lower part of the plant body. The sealed container is fixed on a shaking table and continuously shaken during the culture period. The medium A is constantly stirred to continuously supply nutrients to the crown portion. Cultivate and grow for a week. (See Figure 1)
[0008]
When strawberry seedlings were grown in this way, the average increased about 130 times. Further, the proliferated strawberry seedlings are separated and cut at 2 cm above and below the crown part, and after removing the root part 1a, the leaf part 1b and the stem part 1c not including the crown part 1d, the strawberry seedlings are cut including the crown part. It is further cultured and propagated as a plant body 1 in which the strawberry seedling is regenerated.
[0009]
Three of the grown seedlings were cut at 2 cm above and below the crown part, and after removing the root part 1a, the leaf part 1b, and the stem part 1c not including the crown part 1d, the liquid medium was similarly used. 4 is put in a sealed container 3 and fixed on a shaking table, and while being continuously shaken during the culture period, the accommodated seedlings are cultured for 9 weeks to proliferate and extend. At this time, the medium B was used as the liquid medium 4.
[0010]
When strawberry seedlings were cultured in this manner, the number of plants having a plant height of 15 mm or more (healthy seedlings) increased to an average of about 130 per container, and extended to an easily handleable length of 2 cm or more in general.
[0011]
Next, the expanded seedling is fixed in a sealed container 3 together with the liquid medium 4 and is continuously shaken during the culture period. The liquid medium 4 is constantly stirred to supply nutrients to the crown portion. Then, the accommodated plant body is cultured and grown for 5 weeks. At this time, the medium C was used as the liquid medium 4.
[0012]
It was found that when the strawberry seedlings were grown in this way, the seedlings were strong reddish. Further, the enlarged strawberry seedlings are stocked and transplanted to a culture soil, then acclimatized to grow strawberry fruits and transplanted in a natural environment.
[0013]
The shaking table used here can rotate the sealed container 3 fixed on the shaking table around a vertical axis so as to draw a circle on a horizontal plane, and stir the culture medium 4 in the sealed container 3. It is. Moreover, the sealed container used here means a container that allows gas to enter and exit inside and outside and blocks invasion of germs and the like.
[0014]
As liquid medium A, MS medium (described later) is diluted 4-fold (hereinafter referred to as 1 / 4MS) (hereinafter referred to as 1 / nMS refers to MS medium diluted n-fold). It is a medium in which glucose is added at 1% by weight and BA is added at 0.03 mg / liter. In the liquid medium B, as a liquid fertilizer having a ratio of nitrogen, phosphorus and potassium (N: P: K) of 6.5: 6: 19, a 0.1% aqueous solution of fine powder Hyponex (manufactured by Hyponex) is used. A medium containing a trace element of MS medium (described later) and a vitamin element of MS medium (described later) as a basic medium (hereinafter referred to as HY medium), 0.5% to 3% glucose, and What contained 30 mg / liter of iron diaminetetraacetate and 0.5 mg / liter or less of benzyladenine (BA) was used. In addition, as the liquid medium C, a medium in which 4% by weight of glucose and BA were added to a 1/4 MS medium so as to be 0.03 mg / liter was used.
[0015]
The composition of the MS medium, and the composition of the trace elements and vitamin elements of the MS medium are shown below in terms of weight per liter (unit: mg).
[0016]
Figure 0004154458
In addition, when including ethylenediaminetetraacetic acid iron, iron sulfate heptahydrate and ethylenediaminetetraacetic acid disodium are omitted.
[0017]
Figure 0004154458
[0018]
Vitamin elements of MS medium:
Nicotinic acid …………………… 0.5
Thiamine hydrochloride …………………… 0.1
Pyridoxine hydrochloride …………………… 0.5
m-Inositol …………………… 100
Glycine …………………… 2
[0019]
【Example】
Embodiments of the present invention will be described below with reference to the drawings.
Three strawberry seedlings 1 obtained by cultivating the growing point according to a conventional method are sealed in sealed containers 3 each containing 50 ml of medium A1, A2, D1 to D4, and liquid medium using the shaking table. Strawberry seedlings were cultured for 35 days under the condition of constantly stirring No. 4 to examine the number of seedlings grown.
The composition of each medium is as shown in Table 1.
As a result, it became as shown in Table 2.
[0020]
[Table 1]
Figure 0004154458
[0021]
[Table 2]
Figure 0004154458
[0022]
That is, when the strawberry seedling 1 is cultured and proliferated with the liquid medium composition A1 described above, it is found that the strawberry seedling 1 grows about 130 times although there is variation due to experimental error. It was found that the growth property was higher than that when using.
In addition, when the liquid medium 4 (mediums D2 to D5) supplemented with kinetin is used for 1/4 MS, the growth rate is generally lower than when BA is used, but when BA is used. The growth rate was comparable. In other words, a high growth rate can be obtained by using liquid medium 4 with kinetin added to 1/2 MS to 1/10 MS, and if BA is added to this medium, an improvement in growth rate over Test Example 1 is expected. I can do it. It is empirically known that kinetin has the same effect as BA when used in a range of 1 mg / liter or less.
[0023]
Each of the three strawberry seedlings 1 grown by the above method is sealed in a sealed container 3 containing 50 ml of each of medium B1, B2, D6, and D7, and liquid medium 4 is constantly stirred using the shaking table. Under the continued conditions, culture was performed to examine the number of strawberry seedlings of 15 mm or more.
Each medium is obtained by changing the type and concentration of the basic medium of the liquid medium A1 as shown in Table 3.
[0024]
As a result, the average number of growth was as shown in Table 4.
The culture period is 9 weeks.
[0025]
[Table 3]
Figure 0004154458
[0026]
[Table 4]
Figure 0004154458
[0027]
[Test Example 2]
Strawberry seedlings were cultured for 6 weeks in the same manner as in Test Example 1 using the liquid medium D and liquid medium E shown in Table 3, and the number of seedlings of 15 mm or more was examined. As a result, it became as shown in Table 4.
[0028]
From Table 4, it can be seen that when the HY medium is used, the proportion of relatively long shoots extending to 25 mm to 35 mm is high, and many long shoots that are easy to handle are also cultured. Similarly, strawberry seedlings were cultured for 6 weeks using the liquid media B2 and B3 shown in Table 5, and the number of seedlings of 15 mm or more was examined. As a result, it became as shown in Table 6.
[0029]
[Table 5]
Figure 0004154458
[0030]
[Table 6]
Figure 0004154458
[0031]
As a result, it was found that glucose is preferable as sucrose as the sugar used in the liquid medium composition used in the extension phase.
[0032]
Next, the strawberry seedling 1 elongated by the previous culture is sealed in a sealed container 3 with 50 ml of each of the media C1 to C5, and the liquid medium 4 is constantly stirred using the shaking table. After culturing, the number of strawberry seedlings with a plant height of 15 mm or more and thick reddish and thickened was examined. The composition of each medium is as shown in Table 3.
[0033]
As a result, the average number of growth was as shown in the figure.
[0034]
[Table 7]
Figure 0004154458
[0035]
FIG. 2 shows that when a ¼ MS medium with a high sucrose concentration is used, the proportion of buds enlarged at 25 mm to 35 mm is high.
Moreover, when each 50 ml of each culture medium C6-C8 was enclosed with each sealed container separately and culture | cultivated similarly, it became like FIG. As can be seen from FIG. 3, the sugar contained in the medium is preferably glucose rather than sucrose, and the sugar concentration is preferably about 3 to 5%.
[Brief description of the drawings]
FIG. 1 is a process chart showing a strawberry culture method. FIG. 2 is a graph showing the dependency of strawberry enlargement on sucrose concentration. FIG. 3 is a graph showing the dependency of strawberry enlargement on sugar content.

Claims (1)

イチゴの培養方法であって、
イチゴの苗の芽の数を増やす増殖期に、2〜10倍希釈MS液体培地に0.5%以上2%未満のブドウ糖、及び、0.5mg/リットル以下のベンジルアデニン(BA)を含ませてある培地で前記イチゴの苗を培養し、
前記増殖したイチゴの苗を伸長させる伸長期に、窒素、燐、カリウムの比(N:P:K)が、6.5:6:19である肥料に、MS培地の微量要素とMS培地のビタミン類要素、及び、30mg/リットルのエチレンジアミン四酢酸鉄、及び、0.5%以上3%以下の糖、及び、0.5mg/リットル以下のベンジルアデニン(BA)を含有させてある液体培地で前記イチゴの苗を培養し、
その後、前記伸長したイチゴの苗を地植え出来るように成長させる肥大期に、2〜10倍希釈MS培地に、2%以上5%以下のブドウ糖もしくはショ糖、及び、0.5mg/リットル以下のベンジルアデニン(BA)を含有させてある液体培地で前記イチゴの苗を培養するイチゴ培養方法。
A method for cultivating strawberries,
In the growth phase to increase the number of shoots of strawberry seedlings, 0.5 to 2% glucose and 0.5 mg / liter or less of benzyladenine (BA) are added to the MS liquid medium diluted 2 to 10 times. Cultivate the strawberry seedling in a certain medium,
In the extension period for extending the grown strawberry seedlings, fertilizers with a ratio of nitrogen, phosphorus, and potassium (N: P: K) of 6.5: 6: 19 were added to the trace elements of the MS medium and the MS medium. A liquid medium containing vitamin elements, 30 mg / liter of iron diaminetetraacetate, 0.5% to 3% of sugar, and 0.5 mg / liter or less of benzyladenine (BA) Cultivating the strawberry seedling,
Then, in the hypertrophy stage where the seedlings of the elongated strawberries are grown so that they can be planted in the ground, 2% to 5% diluted MS medium with 2% to 5% glucose or sucrose, and 0.5 mg / liter or less A strawberry culturing method, wherein the strawberry seedling is cultured in a liquid medium containing benzyladenine (BA).
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