JP4029280B2 - Sample crusher - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a sample crushing tool capable of crushing a sample while being put in a container.
[0002]
[Prior art]
When nucleic acids are extracted from animal and plant tissues, the sample is pretreated by enzyme treatment or the like, but there is a problem that the treatment takes time and effort.
[0003]
Therefore, a sample crushing tool has been proposed in which one crusher 101 is slidably accommodated in a sealed container 100 as shown in FIG. 10 (see, for example, Patent Document 1). According to such a sample crushing tool, the crusher 101 slides in the container 100 by moving the container 100 up and down as shown in FIG. 11, and the crusher 101 collides with the sample S that has entered between the container 100 and the crusher 101. Since the sample S can be pulverized, the pretreatment for nucleic acid extraction can be easily performed, and the advantage that the sample S can be handled in a frozen state by precooling the sample S with liquid nitrogen. There is. Furthermore, since the pulverized sample S does not scatter, it is effective for pulverizing mold, yeast, organs and the like.
[0004]
However, when the hard sample S is pulverized, there is a problem that the number of times the container 100 is shaken increases, and the pulverization processing of the sample S takes a long time.
[0005]
[Patent Document 1]
JP 11-148890 A (all pages, all figures)
[0006]
[Problems to be solved by the invention]
An object of the present invention is to provide a sample crushing tool that can crush a sample in a short time and has a simple structure.
[0007]
[Means for Solving the Problems]
The sample crusher of the present invention is a sample crusher comprising a cylindrical container and a crusher housed in the container so as to be slidable along the axis of the container. Are arranged in order of weight along the axis.
[0008]
In addition, it is desirable to form an uneven portion for sample crushing on one of the opposing surfaces of the adjacent crusher.
[0009]
In addition, it is desirable to form a meshed uneven portion on each of the opposing surfaces of the adjacent crushers.
[0010]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, embodiments of the present invention will be described with reference to the drawings.
[0011]
1 to 4 show a sample crushing tool according to the first embodiment, and the sample crushing tool is configured such that a crusher 2 is slidably accommodated in a container 1.
[0012]
The container 1 includes a cylindrical container body 4 having a distal end side closed and a sample S insertion / removal port 3 formed on the proximal end side, and a lid body 5 that seals the container 1 by closing the insertion / removal port 3. . For the purpose of facilitating the suction (recovery) of the sample S by a pipette, the distal end side of the container body 4 is formed in a conical shape, and a male screw 6 is formed on the outer peripheral surface of the proximal end portion of the container body 4. Yes. On the inner peripheral surface of the lid 5, a female screw 7 that is screwed with the male screw 6 of the container body 4 is formed. In addition, although the container 1 is the synthetic resin disposable container 1, you may form with corrosion-resistant metals, ceramics, etc., such as stainless steel.
[0013]
The crusher 2 includes a first crusher 21 and a second crusher 22 that is lighter than the first crusher 21. The first crusher 21 is formed of a columnar portion formed along the inner wall surface of the cylindrical portion of the container body 4 and a conical portion that matches the tip portion of the container body 4. The outer diameters of the columnar portions of the first crusher 21 and the second crusher 22 are set to be slightly smaller than the inner diameter of the cylindrical portion of the container body 4. The second crusher 22 is formed in a columnar shape so as to follow the inner wall surface of the cylindrical portion of the container body 4. The first and second crushers 21 and 22 are made of a corrosion-resistant metal such as stainless steel, ceramics, or the like. The first and second crushers 21 and 22 are accommodated in the container 1 so as to be slidable along the axis of the container body 4.
[0014]
Regarding the dimensions of the sample crushing tool, for example, the container body 4 has a length of 115 mm, the cylindrical portion has an outer diameter of 29 mm and an inner diameter of 27.5 mm, and the first crusher 21 has a length of 33 mm and an outer diameter. The second crusher 22 has a length of 20 mm and an outer diameter of 25 mm.
[0015]
When the sample crusher configured in this way is shaken up and down or left and right, the crusher 2 reciprocates in the container body 4. When the crusher 2 moves from the front end side of the container 1 to the lid body 5 side as shown in FIGS. 2A and 2B, the lightweight second crusher 22 moves faster than the first crusher 21 and moves to the lid body 5. Then, the first crusher 21 collides with the second crusher 22. When the crusher 2 slides from the lid 5 side to the tip side of the container 1 as shown in FIGS. 2C and 2D, the first and second crushers 21 and 22 move in contact or close to each other. To do.
[0016]
Therefore, when the sample S is inserted between the first crusher 21 and the second crusher 22 as shown in FIG. 3, the second crusher 22 collides with the sample S when the crusher 2 moves to the lid 5 side. Then, the sample S is pulverized, and when the crusher 2 moves to the front end side of the container 1, the first and second crushers 21 and 22 sandwiching the sample S collide with the front end portion of the container 1 and the sample S Is pushed by the first crusher 21, and the sample S can be crushed by the striking action and the compressing action.
[0017]
When the sample S is inserted between the second crusher 22 and the lid 5 as shown in FIG. 4, the second crusher 22 collides with the sample S when the crusher 2 moves to the lid 5 side. After that, when the first crusher 21 collides with the second crusher 22, the sample S can be pulverized by being hit twice. Note that the sample S is not crushed when the crusher 2 slides toward the lid 5 side of the container 1.
[0018]
As described above, in any case, the crusher 2 makes one reciprocation in the container 1 so that the sample S can be subjected to two impacts. Therefore, like the conventional sample crushing tool described above, one reciprocation is made. Compared with a single impact, the grinding efficiency is improved.
[0019]
Furthermore, if the sample S is placed between the container 1 and the first crusher 21 and between the first crusher 21 and the second crusher 22, the amount of the sample S that can be processed at a time can be increased. .
[0020]
Further, the crushers 2 may be arranged in order of weight so that the weight of the crusher 2 closer to the lid 5 is increased.
[0021]
After the sample S is stored in the container 1, the sample pulverizing tool is put into liquid nitrogen and cooled, and the sample pulverizing tool is shaken by hand or using a vibration device. Further, the sample S may be crushed by hitting the sample crushing tool against the impact body. After completion of the crushing operation, the lid of the sample crusher is opened and the sample S and the crusher 2 are taken out.
[0022]
FIG. 5 shows a sample crusher according to the second embodiment, in which first to third crushers 21, 22, and 23 having different weights are arranged in order of weight along the axis of the container body 4. According to such a configuration, by inserting the sample S between the lid 5 and the third crusher 23 as shown in FIG. 6, after the lightest third crusher 23 collides with the lid 5, the second crusher 22. And the third crusher 23 collide one after another, and the sample S is impacted three times and pulverized. The sample S may be placed between the third crusher 23 and the second crusher 22 or between the second crusher 22 and the first crusher 21.
[0023]
FIG. 7 shows a sample pulverizing tool according to the third embodiment, in which a concavo-convex portion 6 that can be engaged with the opposing surfaces of the first crusher 21 and the second crusher 22 is provided. According to this sample crushing tool, when the crushers 2 collide with each other, the cutting action of the sample S by the convex portions of the concavo-convex portions 6 is further added, and the pulverizing effect can be improved. Further, by providing a guide for preventing the crusher 2 from rotating in the container body 4 around the axis, the concave and convex portions 6 may be surely engaged with each other. Note that the sample S can be pulverized even if the crusher 2 rotates in the container body 4 and the engagement of the concavo-convex portion 6 becomes worse. Moreover, you may form an uneven | corrugated | grooved part only in one of the opposing surfaces of the adjacent crusher 2. FIG.
[0024]
FIG. 8 shows a sample crusher according to the fourth embodiment, in which a spherical concave portion 7 is provided on the end surface of the first crusher 21, and a spherical convex portion that matches the concave portion 9 on the end surface of the second crusher 22. 8 is provided. FIG. 9 shows a sample crushing tool according to the fifth embodiment. The end surface of the first crusher 21 is inclined, and the end surface of the second crusher 22 is also inclined so as to coincide with the end surface of the first crusher 21. According to these sample crushing tools, when the second crusher 22 of the first crusher 21 collides, a crushing force such as a shearing force is applied simultaneously with a force for crushing the sample S to enhance a crushing effect. it can. In addition, you may make it provide the above uneven | corrugated | grooved parts in the opposing surface where the crusher 2 inclined.
[0025]
【The invention's effect】
According to the sample crushing tool of the present invention, a plurality of crushers having different weights are arranged in order of weight along the axis of the container, so that the crushing efficiency of the sample can be improved and the sample that can be crushed at once The amount of can be increased.
[0026]
In addition, if a concavo-convex portion for pulverization is formed on one of the opposing surfaces of adjacent crushers, a cutting force can act when the crusher collides with the sample, thereby improving the pulverization effect.
[0027]
Further, if a meshed uneven portion is formed on each of the adjacent surfaces of the crusher, the crushing effect of the sample can be improved by meshing the uneven portion.
[Brief description of the drawings]
FIG. 1 is an exploded perspective view of a sample crusher according to a first embodiment of the present invention.
2A is a cross-sectional view of a sample crusher according to the embodiment, and FIGS. 2B, 2C, and 2D are cross-sectional views illustrating the operation of the sample crusher according to the embodiment. FIG.
FIG. 3 is a cross-sectional view showing a state in which a sample of the sample crusher according to the embodiment is placed.
FIG. 4 is a cross-sectional view showing a state in which a sample of the sample crusher according to the embodiment is placed.
FIG. 5 is a cross-sectional view of a sample crusher according to a second embodiment of the present invention.
FIG. 6 is a cross-sectional view showing a state in which a sample is put in the sample grinder according to the embodiment.
FIG. 7 is an exploded perspective view of a sample crusher according to a third embodiment of the present invention.
FIG. 8 is a cross-sectional view of a sample crusher according to a fourth embodiment of the present invention.
FIG. 9 is a cross-sectional view of a sample crusher according to a fifth embodiment of the present invention.
FIG. 10 is a cross-sectional view of a conventional sample grinder.
FIG. 11 is a cross-sectional view illustrating the operation of a conventional sample crusher.
[Explanation of symbols]
1 Container 21 First Crusher 22 Second Crusher S Sample

Claims (3)

In a sample crushing tool comprising a cylindrical container and a crusher stored in the container so as to be slidable along the axis of the container,
A sample crusher characterized in that a plurality of crushers having different weights are arranged in order of weight along the axis. The sample crushing tool according to claim 1, wherein an uneven portion for crushing the sample is formed on one of the opposing surfaces of the adjacent crushers. The sample crushing tool according to claim 1, wherein a concavo-convex portion capable of meshing is formed on each of the opposing surfaces of the adjacent crushers.

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