JP3788909B2 - 親和力配位子について複合生理学的材料をスクリーニングする高処理量のサイズ排除法 - Google Patents

親和力配位子について複合生理学的材料をスクリーニングする高処理量のサイズ排除法 Download PDF

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JP3788909B2
JP3788909B2 JP2000598857A JP2000598857A JP3788909B2 JP 3788909 B2 JP3788909 B2 JP 3788909B2 JP 2000598857 A JP2000598857 A JP 2000598857A JP 2000598857 A JP2000598857 A JP 2000598857A JP 3788909 B2 JP3788909 B2 JP 3788909B2
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reaction mixture
target
size exclusion
ligand
molecular weight
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JP2002541435A (ja
JP2002541435A5 (enExample
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ドナエフスキー，ユーリー，エム．
ヒュース，ダラス，イー．
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セテク コーポレイション
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/538—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip, i.e. sorbent materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B01J2219/00707—Processes involving means for analysing and characterising the products separated from the reactor apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/468—Flow patterns using more than one column involving switching between different column configurations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
JP2000598857A 1999-02-12 2000-02-11 親和力配位子について複合生理学的材料をスクリーニングする高処理量のサイズ排除法 Expired - Fee Related JP3788909B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11996699P 1999-02-12 1999-02-12
US60/119,966 1999-02-12
PCT/US2000/003562 WO2000047999A1 (en) 1999-02-12 2000-02-11 High throughput size-exclusive method of screening complex biological materials for affinity ligands

Publications (3)

Publication Number Publication Date
JP2002541435A JP2002541435A (ja) 2002-12-03
JP2002541435A5 JP2002541435A5 (enExample) 2005-10-13
JP3788909B2 true JP3788909B2 (ja) 2006-06-21

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JP2000598857A Expired - Fee Related JP3788909B2 (ja) 1999-02-12 2000-02-11 親和力配位子について複合生理学的材料をスクリーニングする高処理量のサイズ排除法

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US (1) US7179592B2 (enExample)
EP (1) EP1151301A4 (enExample)
JP (1) JP3788909B2 (enExample)
CA (1) CA2362052A1 (enExample)
WO (1) WO2000047999A1 (enExample)

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US6528098B2 (en) * 1996-10-22 2003-03-04 Advanced Viral Research Corp. Preparation of a therapeutic composition
US6837977B1 (en) * 1999-06-24 2005-01-04 Cetek Corporation Capillary electrophoresis method for screening for affinity ligands using a detectable competitive ligand
CA2401014A1 (en) * 2000-02-25 2001-08-30 American Home Products Corporation Methods of structure-based drug design using ms/nmr
EP1344060A4 (en) * 2000-11-17 2004-12-22 Alfred E Slanetz METHOD FOR DETERMINING THE FUNCTION OF TARGETS AND IDENTIFYING SERIAL HEADS OF DRUGS
DE10125258A1 (de) * 2001-05-23 2003-01-09 November Ag Molekulare Medizin Verfahren zur Bestimmung des Bindeverhaltens von an Ziel-Molekülen spezifisch bindenden Liganden
US6833238B2 (en) 2002-01-04 2004-12-21 Applera Corporation Petal-array support for use with microplates
CA2486486A1 (en) * 2002-05-17 2004-05-06 Alfred E. Slanetz Process for determining target function and identifying drug leads
US20040016702A1 (en) * 2002-07-26 2004-01-29 Applera Corporation Device and method for purification of nucleic acids
US20050181378A1 (en) * 2004-02-18 2005-08-18 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050196856A1 (en) * 2004-02-18 2005-09-08 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20060160122A1 (en) * 2004-02-18 2006-07-20 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
WO2006015796A1 (en) * 2004-08-09 2006-02-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Method for high-throughput screening of test molecules binding to target molecules
US7169425B2 (en) 2004-09-17 2007-01-30 Solae, Llc Size exclusion chromatography process for the preparation of an improved soy protein-containing composition
US20060062889A1 (en) * 2004-09-17 2006-03-23 Solae, Llc. Soy protein-containing composition
JP4735289B2 (ja) * 2005-01-31 2011-07-27 株式会社島津製作所 標的化合物と結合する反応化合物を特定するスクリーニング方法及び装置
US20060169640A1 (en) * 2005-02-01 2006-08-03 Hubert Quinn High throughput screening, purification and recovery system for large and small molecules
ES2391278T3 (es) * 2005-06-03 2012-11-23 Ohr Pharmaceutical, Inc. Métodos para proporcionar cuidado paliativo con Producto R
WO2010053941A2 (en) * 2008-11-04 2010-05-14 Duke University Affinity displacement molecular separation
US9891149B2 (en) 2011-08-08 2018-02-13 Thermo Fisher Scientific Oy Method and apparatus for automated analysis
FI20115785A0 (fi) * 2011-08-08 2011-08-08 Thermo Fisher Scientific Oy Menetelmä ja laite automaattiseen analyysiin
CN102818868B (zh) * 2012-08-27 2013-11-20 浙江大学 复杂天然产物中活性成分筛选方法及应用
US11071927B2 (en) * 2017-11-15 2021-07-27 Arup Laboratories, Inc. Separating and quantifying unbound target analytes from biological samples
JP2022547309A (ja) * 2019-09-10 2022-11-11 ピアス・バイオテクノロジー・インコーポレイテッド サンプル調製組成物、デバイス、システムおよび方法
CN112649517B (zh) * 2019-10-12 2021-10-15 中国科学院大连化学物理研究所 一种从生物体代谢物中筛选靶蛋白配体的方法
JP7327096B2 (ja) * 2019-11-13 2023-08-16 株式会社島津製作所 分子の分離方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891742A (en) * 1995-01-19 1999-04-06 Chiron Corporation Affinity selection of ligands by mass spectroscopy
US5630924A (en) 1995-04-20 1997-05-20 Perseptive Biosystems, Inc. Compositions, methods and apparatus for ultrafast electroseparation analysis
EP0835446B1 (en) * 1995-06-26 2003-04-09 Perseptive Biosystems, Inc. High speed, automated, continuous flow, multi-dimensional molecular selection and analysis
US5783397A (en) 1995-12-11 1998-07-21 Northeastern University Screening natural samples for new therapeutic compounds using capillary electrophoresis
US6207861B1 (en) * 1998-01-05 2001-03-27 Neogenesis, Inc. Method for producing and screening mass coded combinatorial libraries for drug discovery and target validation
DE10028186A1 (de) * 2000-06-09 2002-09-19 November Ag Molekulare Medizin Verfahren zur Bestimmung der Menge von an Ziel-Molekülen gebundenen Liganden

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Publication number Publication date
US7179592B2 (en) 2007-02-20
JP2002541435A (ja) 2002-12-03
WO2000047999A1 (en) 2000-08-17
EP1151301A1 (en) 2001-11-07
CA2362052A1 (en) 2000-08-17
EP1151301A4 (en) 2004-07-14
US20020052006A1 (en) 2002-05-02

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